LOCUS       DANJJH010000001       127140 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000001 DANJJH010000000
VERSION     DANJJH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 127140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..378
                     /locus_tag="PWS53_000001"
     CDS             <1..378
                     /locus_tag="PWS53_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0870282.1"
                     /translation="KKEKLPIDFRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQL
                     FEKHSGRQMTIATSNKSMVLFSTTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPK
                     FGSINLHPGQERISQTIYRFSAQ"
     gene            400..1419
                     /locus_tag="PWS53_000002"
     CDS             400..1419
                     /locus_tag="PWS53_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0870283.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1700..3067)
                     /gene="nhaC"
                     /locus_tag="PWS53_000003"
     CDS             complement(1700..3067)
                     /gene="nhaC"
                     /locus_tag="PWS53_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0870284.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(3177..4598)
                     /locus_tag="PWS53_000004"
     CDS             complement(3177..4598)
                     /locus_tag="PWS53_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0870285.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(4841..6718)
                     /gene="tdc"
                     /locus_tag="PWS53_000005"
     CDS             complement(4841..6718)
                     /gene="tdc"
                     /locus_tag="PWS53_000005"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0870286.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(7009..8265)
                     /locus_tag="PWS53_000006"
     CDS             complement(7009..8265)
                     /locus_tag="PWS53_000006"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL0870287.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(8377..8582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8845..10146
                     /locus_tag="PWS53_000007"
     CDS             8845..10146
                     /locus_tag="PWS53_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0870288.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(10215..10814)
                     /locus_tag="PWS53_000008"
     CDS             complement(10215..10814)
                     /locus_tag="PWS53_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0870289.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            11068..11949
                     /locus_tag="PWS53_000009"
     CDS             11068..11949
                     /locus_tag="PWS53_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870290.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            11962..13572
                     /locus_tag="PWS53_000010"
     CDS             11962..13572
                     /locus_tag="PWS53_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL0870291.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(13649..14308)
                     /locus_tag="PWS53_000011"
     CDS             complement(13649..14308)
                     /locus_tag="PWS53_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0870292.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(14591..15256)
                     /locus_tag="PWS53_000012"
     CDS             complement(14591..15256)
                     /locus_tag="PWS53_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL0870293.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(15403..16008)
                     /gene="dhaL"
                     /locus_tag="PWS53_000013"
     CDS             complement(15403..16008)
                     /gene="dhaL"
                     /locus_tag="PWS53_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL0870294.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(16036..17046)
                     /gene="dhaK"
                     /locus_tag="PWS53_000014"
     CDS             complement(16036..17046)
                     /gene="dhaK"
                     /locus_tag="PWS53_000014"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL0870295.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(17021..17467)
                     /gene="dhaM"
                     /locus_tag="PWS53_000015"
     CDS             complement(17021..17467)
                     /gene="dhaM"
                     /locus_tag="PWS53_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL0870296.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            17570..17890
                     /locus_tag="PWS53_000016"
     CDS             17570..17890
                     /locus_tag="PWS53_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL0870297.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(18002..18871)
                     /locus_tag="PWS53_000017"
     CDS             complement(18002..18871)
                     /locus_tag="PWS53_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0870298.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(18967..20270)
                     /locus_tag="PWS53_000018"
                     /pseudo
     CDS             complement(18967..20270)
                     /locus_tag="PWS53_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(20267..20776)
                     /locus_tag="PWS53_000019"
     CDS             complement(20267..20776)
                     /locus_tag="PWS53_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870299.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(20951..23086)
                     /locus_tag="PWS53_000020"
     CDS             complement(20951..23086)
                     /locus_tag="PWS53_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0870300.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(23169..24824)
                     /locus_tag="PWS53_000021"
     CDS             complement(23169..24824)
                     /locus_tag="PWS53_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0870301.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(24826..25140)
                     /locus_tag="PWS53_000022"
     CDS             complement(24826..25140)
                     /locus_tag="PWS53_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0870302.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            25310..25570
                     /locus_tag="PWS53_000023"
     CDS             25310..25570
                     /locus_tag="PWS53_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL0870303.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            25584..25898
                     /locus_tag="PWS53_000024"
     CDS             25584..25898
                     /locus_tag="PWS53_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL0870304.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(26628..27182)
                     /locus_tag="PWS53_000025"
     CDS             complement(26628..27182)
                     /locus_tag="PWS53_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL0870305.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(27308..29236)
                     /locus_tag="PWS53_000026"
     CDS             complement(27308..29236)
                     /locus_tag="PWS53_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0870306.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(29542..30258)
                     /locus_tag="PWS53_000027"
     CDS             complement(29542..30258)
                     /locus_tag="PWS53_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0870307.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(30259..32082)
                     /gene="glpO"
                     /locus_tag="PWS53_000028"
     CDS             complement(30259..32082)
                     /gene="glpO"
                     /locus_tag="PWS53_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL0870308.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(32096..33592)
                     /gene="glpK"
                     /locus_tag="PWS53_000029"
     CDS             complement(32096..33592)
                     /gene="glpK"
                     /locus_tag="PWS53_000029"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL0870309.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(33728..35188)
                     /locus_tag="PWS53_000030"
     CDS             complement(33728..35188)
                     /locus_tag="PWS53_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870310.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(35178..36473)
                     /locus_tag="PWS53_000031"
     CDS             complement(35178..36473)
                     /locus_tag="PWS53_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0870311.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(36805..37482)
                     /locus_tag="PWS53_000032"
     CDS             complement(36805..37482)
                     /locus_tag="PWS53_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL0870312.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(37512..38603)
                     /locus_tag="PWS53_000033"
     CDS             complement(37512..38603)
                     /locus_tag="PWS53_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL0870313.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(38704..39138)
                     /locus_tag="PWS53_000034"
     CDS             complement(38704..39138)
                     /locus_tag="PWS53_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0870314.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(39722..41179)
                     /gene="ascB"
                     /locus_tag="PWS53_000035"
     CDS             complement(39722..41179)
                     /gene="ascB"
                     /locus_tag="PWS53_000035"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0870315.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(41176..43071)
                     /locus_tag="PWS53_000036"
     CDS             complement(41176..43071)
                     /locus_tag="PWS53_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0870316.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(43129..44904)
                     /locus_tag="PWS53_000037"
     CDS             complement(43129..44904)
                     /locus_tag="PWS53_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0870317.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(44954..46450)
                     /locus_tag="PWS53_000038"
     CDS             complement(44954..46450)
                     /locus_tag="PWS53_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0870318.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(46644..48266)
                     /locus_tag="PWS53_000039"
     CDS             complement(46644..48266)
                     /locus_tag="PWS53_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0870319.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(48715..50028)
                     /locus_tag="PWS53_000040"
     CDS             complement(48715..50028)
                     /locus_tag="PWS53_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0870320.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(50273..50497)
                     /locus_tag="PWS53_000041"
     CDS             complement(50273..50497)
                     /locus_tag="PWS53_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870321.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(50517..50939)
                     /locus_tag="PWS53_000042"
     CDS             complement(50517..50939)
                     /locus_tag="PWS53_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL0870322.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(51083..51793)
                     /locus_tag="PWS53_000043"
     CDS             complement(51083..51793)
                     /locus_tag="PWS53_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870323.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(51811..52308)
                     /locus_tag="PWS53_000044"
     CDS             complement(51811..52308)
                     /locus_tag="PWS53_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870324.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(52345..52797)
                     /locus_tag="PWS53_000045"
     CDS             complement(52345..52797)
                     /locus_tag="PWS53_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0870325.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(52932..53537)
                     /locus_tag="PWS53_000046"
     CDS             complement(52932..53537)
                     /locus_tag="PWS53_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0870326.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(53987..54607)
                     /locus_tag="PWS53_000047"
     CDS             complement(53987..54607)
                     /locus_tag="PWS53_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0870327.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(54609..55478)
                     /locus_tag="PWS53_000048"
     CDS             complement(54609..55478)
                     /locus_tag="PWS53_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL0870328.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(55501..55908)
                     /locus_tag="PWS53_000049"
     CDS             complement(55501..55908)
                     /locus_tag="PWS53_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0870329.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(55927..57216)
                     /locus_tag="PWS53_000050"
     CDS             complement(55927..57216)
                     /locus_tag="PWS53_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0870330.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(57243..57545)
                     /locus_tag="PWS53_000051"
     CDS             complement(57243..57545)
                     /locus_tag="PWS53_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0870331.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(57570..59663)
                     /locus_tag="PWS53_000052"
     CDS             complement(57570..59663)
                     /locus_tag="PWS53_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0870332.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(60152..60916)
                     /locus_tag="PWS53_000053"
     CDS             complement(60152..60916)
                     /locus_tag="PWS53_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL0870333.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(61027..61896)
                     /gene="mdcB"
                     /locus_tag="PWS53_000054"
     CDS             complement(61027..61896)
                     /gene="mdcB"
                     /locus_tag="PWS53_000054"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL0870334.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(61921..62997)
                     /locus_tag="PWS53_000055"
     CDS             complement(61921..62997)
                     /locus_tag="PWS53_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL0870335.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(63021..63614)
                     /locus_tag="PWS53_000056"
     CDS             complement(63021..63614)
                     /locus_tag="PWS53_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL0870336.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(63618..65294)
                     /gene="mdcD"
                     /locus_tag="PWS53_000057"
     CDS             complement(63618..65294)
                     /gene="mdcD"
                     /locus_tag="PWS53_000057"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL0870337.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(65284..65583)
                     /locus_tag="PWS53_000058"
     CDS             complement(65284..65583)
                     /locus_tag="PWS53_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL0870338.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(65685..67331)
                     /gene="mdcA"
                     /locus_tag="PWS53_000059"
     CDS             complement(65685..67331)
                     /gene="mdcA"
                     /locus_tag="PWS53_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL0870339.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(67338..68249)
                     /locus_tag="PWS53_000060"
     CDS             complement(67338..68249)
                     /locus_tag="PWS53_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0870340.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            68369..69226
                     /locus_tag="PWS53_000061"
     CDS             68369..69226
                     /locus_tag="PWS53_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0870341.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(69413..69775)
                     /locus_tag="PWS53_000062"
     CDS             complement(69413..69775)
                     /locus_tag="PWS53_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL0870342.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            70121..71560
                     /locus_tag="PWS53_000063"
     CDS             70121..71560
                     /locus_tag="PWS53_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870343.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(71567..74119)
                     /locus_tag="PWS53_000064"
     CDS             complement(71567..74119)
                     /locus_tag="PWS53_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL0870344.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(74233..75564)
                     /locus_tag="PWS53_000065"
     CDS             complement(74233..75564)
                     /locus_tag="PWS53_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0870345.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(75530..77008)
                     /locus_tag="PWS53_000066"
     CDS             complement(75530..77008)
                     /locus_tag="PWS53_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0870346.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(77313..79148)
                     /gene="tdc"
                     /locus_tag="PWS53_000067"
     CDS             complement(77313..79148)
                     /gene="tdc"
                     /locus_tag="PWS53_000067"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0870347.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            79618..81045
                     /locus_tag="PWS53_000068"
     CDS             79618..81045
                     /locus_tag="PWS53_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870348.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(81137..81502)
                     /locus_tag="PWS53_000069"
     CDS             complement(81137..81502)
                     /locus_tag="PWS53_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0870349.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(81499..81864)
                     /locus_tag="PWS53_000070"
     CDS             complement(81499..81864)
                     /locus_tag="PWS53_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0870350.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(82056..82304)
                     /locus_tag="PWS53_000071"
     CDS             complement(82056..82304)
                     /locus_tag="PWS53_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870351.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            82303..82431
                     /locus_tag="PWS53_000072"
     CDS             82303..82431
                     /locus_tag="PWS53_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL0870352.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            82488..84182
                     /locus_tag="PWS53_000073"
     CDS             82488..84182
                     /locus_tag="PWS53_000073"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL0870353.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            84186..84305
                     /locus_tag="PWS53_000074"
                     /pseudo
     CDS             84186..84305
                     /locus_tag="PWS53_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            84282..84455
                     /locus_tag="PWS53_000075"
     CDS             84282..84455
                     /locus_tag="PWS53_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870354.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            84833..85966
                     /locus_tag="PWS53_000076"
     CDS             84833..85966
                     /locus_tag="PWS53_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL0870355.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            85997..86317
                     /locus_tag="PWS53_000077"
     CDS             85997..86317
                     /locus_tag="PWS53_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL0870356.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(86346..86810)
                     /locus_tag="PWS53_000078"
     CDS             complement(86346..86810)
                     /locus_tag="PWS53_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870357.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(86942..88195)
                     /locus_tag="PWS53_000079"
     CDS             complement(86942..88195)
                     /locus_tag="PWS53_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0870358.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(88224..88541)
                     /locus_tag="PWS53_000080"
     CDS             complement(88224..88541)
                     /locus_tag="PWS53_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0870359.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(88567..88887)
                     /locus_tag="PWS53_000081"
     CDS             complement(88567..88887)
                     /locus_tag="PWS53_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0870360.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(89351..90766)
                     /locus_tag="PWS53_000082"
     CDS             complement(89351..90766)
                     /locus_tag="PWS53_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0870361.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(90783..92099)
                     /locus_tag="PWS53_000083"
     CDS             complement(90783..92099)
                     /locus_tag="PWS53_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0870362.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(92249..92947)
                     /locus_tag="PWS53_000084"
     CDS             complement(92249..92947)
                     /locus_tag="PWS53_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL0870363.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(93236..94192)
                     /locus_tag="PWS53_000085"
     CDS             complement(93236..94192)
                     /locus_tag="PWS53_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0870364.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(94305..95138)
                     /gene="msrA"
                     /locus_tag="PWS53_000086"
     CDS             complement(94305..95138)
                     /gene="msrA"
                     /locus_tag="PWS53_000086"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0870365.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(95313..96947)
                     /locus_tag="PWS53_000087"
     CDS             complement(95313..96947)
                     /locus_tag="PWS53_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870366.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(96934..98802)
                     /locus_tag="PWS53_000088"
     CDS             complement(96934..98802)
                     /locus_tag="PWS53_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870367.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            98768..100405
                     /locus_tag="PWS53_000089"
     CDS             98768..100405
                     /locus_tag="PWS53_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870368.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(100550..101290)
                     /gene="truA"
                     /locus_tag="PWS53_000090"
     CDS             complement(100550..101290)
                     /gene="truA"
                     /locus_tag="PWS53_000090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0870369.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(101481..102116)
                     /locus_tag="PWS53_000091"
     CDS             complement(101481..102116)
                     /locus_tag="PWS53_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0870370.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(102263..103504)
                     /gene="sstT"
                     /locus_tag="PWS53_000092"
     CDS             complement(102263..103504)
                     /gene="sstT"
                     /locus_tag="PWS53_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL0870371.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            103702..104700
                     /locus_tag="PWS53_000093"
     CDS             103702..104700
                     /locus_tag="PWS53_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870372.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            104713..106731
                     /locus_tag="PWS53_000094"
     CDS             104713..106731
                     /locus_tag="PWS53_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL0870373.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(106791..107507)
                     /locus_tag="PWS53_000095"
     CDS             complement(106791..107507)
                     /locus_tag="PWS53_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL0870374.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(107734..108276)
                     /locus_tag="PWS53_000096"
     CDS             complement(107734..108276)
                     /locus_tag="PWS53_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0870375.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(108281..108508)
                     /locus_tag="PWS53_000097"
     CDS             complement(108281..108508)
                     /locus_tag="PWS53_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0870376.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(108587..110467)
                     /locus_tag="PWS53_000098"
     CDS             complement(108587..110467)
                     /locus_tag="PWS53_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0870377.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(110656..111270)
                     /locus_tag="PWS53_000099"
     CDS             complement(110656..111270)
                     /locus_tag="PWS53_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870378.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            111412..111972
                     /locus_tag="PWS53_000100"
     CDS             111412..111972
                     /locus_tag="PWS53_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0870379.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(112277..112591)
                     /locus_tag="PWS53_000101"
                     /pseudo
     CDS             complement(112277..112591)
                     /locus_tag="PWS53_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(112845..114480)
                     /locus_tag="PWS53_000102"
                     /pseudo
     CDS             complement(112845..114480)
                     /locus_tag="PWS53_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(115035..115598)
                     /locus_tag="PWS53_000103"
     CDS             complement(115035..115598)
                     /locus_tag="PWS53_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0870380.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(115613..116656)
                     /locus_tag="PWS53_000104"
     CDS             complement(115613..116656)
                     /locus_tag="PWS53_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870381.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(116789..117193)
                     /locus_tag="PWS53_000105"
     CDS             complement(116789..117193)
                     /locus_tag="PWS53_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0870382.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(117426..118061)
                     /locus_tag="PWS53_000106"
     CDS             complement(117426..118061)
                     /locus_tag="PWS53_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0870383.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(118327..119379)
                     /locus_tag="PWS53_000107"
     CDS             complement(118327..119379)
                     /locus_tag="PWS53_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0870384.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(119392..120330)
                     /locus_tag="PWS53_000108"
     CDS             complement(119392..120330)
                     /locus_tag="PWS53_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0870385.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(120781..121431)
                     /locus_tag="PWS53_000109"
     CDS             complement(120781..121431)
                     /locus_tag="PWS53_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870386.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(121531..122040)
                     /locus_tag="PWS53_000110"
     CDS             complement(121531..122040)
                     /locus_tag="PWS53_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL0870387.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(122241..123110)
                     /locus_tag="PWS53_000111"
     CDS             complement(122241..123110)
                     /locus_tag="PWS53_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0870388.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(123130..124251)
                     /locus_tag="PWS53_000112"
     CDS             complement(123130..124251)
                     /locus_tag="PWS53_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL0870389.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(124253..124648)
                     /locus_tag="PWS53_000113"
     CDS             complement(124253..124648)
                     /locus_tag="PWS53_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0870390.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(124667..125473)
                     /locus_tag="PWS53_000114"
     CDS             complement(124667..125473)
                     /locus_tag="PWS53_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0870391.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(125466..126254)
                     /locus_tag="PWS53_000115"
     CDS             complement(125466..126254)
                     /locus_tag="PWS53_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0870392.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(126268..126744)
                     /locus_tag="PWS53_000116"
     CDS             complement(126268..126744)
                     /locus_tag="PWS53_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0870393.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(126816..>127140)
                     /locus_tag="PWS53_000117"
     CDS             complement(126816..>127140)
                     /locus_tag="PWS53_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0870394.1"
                     /translation="LMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHI
                     SCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYD
                     YVKRA"
BASE COUNT        37915 a        26336 c        21236 g        41653 t
ORIGIN      
        1 aaaaaagaaa aactgccaat cgactttaga aaatccccaa cactgcaaga gatccttgca
       61 tgttatccag atggactaga tgatgtattt cttttgcatc atcctaggct ttccaaaact
      121 tccttacaat tattcgagaa gcattctggt cgccaaatga ctatagcaac ttctaataaa
      181 agtatggtat tattttctac gactggattc gaagctgatt tctctgtcaa tggaaaacag
      241 atgcattcaa actacggatt agcaatcgaa ccgcaagaaa tccctgatat cgttcatttc
      301 cccaaatttg gatcgatcaa cctccatcct ggtcaagaac ggatcagtca gacgatttat
      361 cggttctctg cacaataaca agagaaaggt gtttttatca tggctattac cgtaaaagat
      421 gtggcgaaaa aagccggtgt tgccacctcg accgtctctc gtgtcatcaa cgatcatcct
      481 agtatttccg agacaacaaa gaagaaagtc cgaaaaatta tggatgaact aggttatgtt
      541 cctaacatga ctgcccgcaa tctaggaaaa cgcatttcca gtgccatcgg cgtaatctta
      601 ccccctcttg attcaaaaga gcggatcggt aacccatttt atcttgagat catggaagca
      661 atcaacgaag aagcgcggaa ttatgatatg actacagcaa tcgctactgc taaaaatttt
      721 gaattgctgt tagagaacgt ccagcgtatg catttacaaa aacaagtaga tggttttatt
      781 ctagtgtatt cagatagtca agatcctgtc attgattatt tataccaaaa ccacatccct
      841 ttcactttga tcggacagcc ttatcatcat gagaacgaaa tcgtttatgt agataatgat
      901 aaccagcttc taggtaaaca agcaacagaa tttttgatcc agaatggaca taagaatatc
      961 ctgtttgtga caaacacgac tcatgaaagt ttgtatttcg aacgatattt cggttatcaa
     1021 aaagcaatga tgatggctgg tcttcctgcg ttatccagtg tagtactaga gcatccggag
     1081 gattatctgt cctttgagaa aacgttgaaa gaaaacgaag ccacagcatt ggtggtcatt
     1141 gacgatatct tcgcccttcg cacgatgcag ttggcgcaaa tgtacggcta tgtcattcca
     1201 gagacactct ccgtcattag cttcaacaac tcgatcttct caacattaac acacccttac
     1261 cttacaagta tcgatatcga tatctccgaa ttaggacgtg tcgctatgca aaagctgcgc
     1321 gaattgatcc atgaagaatt gagcagtggc gtacgtttgg tcatccctca tcgcttgatc
     1381 aaaagagaga cagtcgttcc tttagaaaac ccatactaaa tagctgactc actaaatacg
     1441 ctgaccattt atcctatggt cagcttttta tgtaaaagaa gatactactt ctaacacatt
     1501 gtttatctgt aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
     1561 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
     1621 atttaaaaag caattttctt gagtttcttc ttatttcttg gagttaaacg cttctgtccc
     1681 agcctctttt ttccttcctt caatttttta cacgtcgacg attcaataaa aaaccgccta
     1741 atattgtaaa gacaggtgca agaatcgaga aaaagacgaa tggcaagtaa tgcaacgcgc
     1801 taactttcat cgttcccata atgaatgtgc cgctgactcc ccacggtacg agagaattga
     1861 cggtcgcacc tgaatccgct agtgttcgtg tcaaataggt ttttgataag ccaacgtcat
     1921 caaaagaacg cttgaatgtt tctcctggaa gaatgatgga cagatactgt tctccaacga
     1981 tcagattgat tccgatacag ctcaaggcag taaacccgac taaacgagaa ggtcggtcca
     2041 tcttttcttt taattcttga acgatggtat caacgatcag atatttgatc agtaacccac
     2101 ctaatgccaa tgcaagtaaa atcaaactag cagaacctaa catactcatg atcccgcctc
     2161 gagaaagcag ttcatctagc tttggatcag gtgtatttgc gacataacca ttcatcaaga
     2221 tgtctgctgc ttttccgacc gtcaaacttt tctcatggat aaaagaaaga acgagagcag
     2281 ctgtcgatcc cgctagtaat gtagggatag ccggaatttt tttgattgcc cccccaaata
     2341 aaataactag tggaatcaat gtccaaggag agatccaaaa gctcgctttc atgttttgga
     2401 tcatttcatt gatactagct agatcggctg aatgactgtt atgtcctaat atcgtataaa
     2461 aaatcaacga aaggacgaat gctgggaaat ccgtataacg cataccagcg atatgttcga
     2521 atagattaac tcctccaata cccgctgcca agttggtcgt atctgataaa ggcgagcagt
     2581 tattccctaa aaaggcaccg gaaacgactg cgcctgtagt cattgcatca ttgaatccaa
     2641 gtaaatgacc gatccccaaa aaagcaattc caatcgtcga aatcgttgta aatgaactgc
     2701 cgacagtcac tccgacaatt gcacaaacaa gaaatgctgt cggtaaaaag aaacgcacag
     2761 aaacaagttt aaatccataa accataatcg tagggatggt tccagataaa atccatgaag
     2821 caactaagac accaatcaaa agaaaaatca ggattggaat gatccctgga gtgattcctt
     2881 tgatgatccc gtcatggatc tcatcccatg tgaacttttt aaatctgcca taaaacaaca
     2941 aacacatgaa agctgcaagg atcgggatat gtggcgagag ctggaatccg atgatcagta
     3001 ctcctaggag tgccaacaac acgatcaata cagcgatact ttcttttaaa gaaatatttt
     3061 tattcatcgt cgatctccta tttcaaatga aaaggccctg ccatgaaaaa actattcact
     3121 tggcaggacc tcctactaaa tcagcgacaa aacgtcggct gaaagactta ttttttttat
     3181 ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa gtgattgatg
     3241 atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc atcactcaat
     3301 gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag catgcagctg
     3361 actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc gaattctgga
     3421 tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat acaataaata
     3481 acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat tacaccataa
     3541 agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct agatacgccg
     3601 tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg agctacttgg
     3661 atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac accaataaat
     3721 accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg acagtcaatc
     3781 aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac atttgctaca
     3841 agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa tacgcctttt
     3901 gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat ttcgatccca
     3961 acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc caagtcaggg
     4021 aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc gactttgaag
     4081 gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc tagccataca
     4141 ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt gatgatccag
     4201 tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc tacattacca
     4261 aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc tgggaacatt
     4321 tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc tgtttttacc
     4381 cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga aaccattgat
     4441 atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt ccaaccgact
     4501 gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag accgataaag
     4561 gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct ccttgatttt
     4621 ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca attcagcgaa
     4681 tatttgaggg gattctttga gtctattgct tgaagggttt cttcaaagac aacctcaaaa
     4741 caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat gcgtcagaaa
     4801 cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct tcgcttgcca
     4861 ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg attttttcag
     4921 catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg gcacgcaata
     4981 cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg ttcacaaatt
     5041 gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt aagaactcat
     5101 taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg ttcaatttat
     5161 tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc atattgaaat
     5221 ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat gacaagtcat
     5281 tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat ttaccatatc
     5341 ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct gttgcccctg
     5401 cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg cctttttcaa
     5461 atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg atgacgatac
     5521 caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca gattctgctt
     5581 cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg ttttctgtaa
     5641 agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag ttgttgtctt
     5701 cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt aagtagaagt
     5761 aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata ccgtcgattg
     5821 ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt ttttcagctg
     5881 ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg tggtcaactg
     5941 gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt agccatgaat
     6001 agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt ccgctacgtg
     6061 ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga ttcatgattt
     6121 cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt tcaggtgtca
     6181 cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt ccttctaagt
     6241 ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc attaattttg
     6301 caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat tcataggcaa
     6361 cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc atcaatgttt
     6421 cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa tgggtacgca
     6481 tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt tcaaaactag
     6541 cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag ttttgacgcc
     6601 agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg ccgttctcag
     6661 ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat tcactcatag
     6721 tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac ctgttcttct
     6781 attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta tcaattatcc
     6841 gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc
     6901 ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt tcaaaattgg
     6961 aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct aatcaattac
     7021 tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac catcgaattc
     7081 atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc
     7141 tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg tggaaacaag
     7201 cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt taccgaatcc
     7261 ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg cttgcgttat
     7321 tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt cttctgccaa
     7381 acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta agtcttcgat
     7441 ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc gatcatcttg
     7501 attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa gccaaacggc
     7561 accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg ggatcgttaa
     7621 accgaacgct tctacttctg gtccttctaa ttttcggatt aggtctaatc cagctgtgat
     7681 atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt tatgcagatg
     7741 caaaaagtcg atagattgca gaatttggta agtaaactct gtaaatgaga ttcctacttc
     7801 caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt ttttaccgta
     7861 gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt tcacaaatgt
     7921 gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt ccacgttatg
     7981 ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg ggtcgccgat
     8041 cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt ggaatctttt
     8101 catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc
     8161 gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt cgtttgtttg
     8221 ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac tgacctccgt
     8281 gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt tcttcaggat
     8341 aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat ctcgcggtac
     8401 cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg ctgtcgcgtg
     8461 gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac tggtttgcag
     8521 cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat cttcgtcttg
     8581 ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca gaaaaaggat
     8641 atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg aacattttat
     8701 tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt tatcgagaag
     8761 tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac aagtaaaaaa
     8821 atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt aggagccact
     8881 tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg atcatctaca
     8941 aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga accgacagat
     9001 gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga ttatgaccaa
     9061 gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca acagcttctt
     9121 caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac tcagatctct
     9181 catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc tcaacaggca
     9241 caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat cgaacaactt
     9301 tatcaaaatg attttgtttt agttaattta aatgatttga tccaaaaaga cgaacaagga
     9361 aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt tctttcacaa
     9421 gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg attggtcgta
     9481 tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga aatcggtgac
     9541 tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt ttcttatcga
     9601 ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta tcgaacatca
     9661 aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc taaaaaagtg
     9721 gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg tcacttgaat
     9781 atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca gcaagaagtt
     9841 gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga catcagcgac
     9901 tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca aggtttcgat
     9961 attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac ggatttctat
    10021 cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga cggaaaaaat
    10081 cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc acgcgaatct
    10141 gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat cgtcagtttc
    10201 ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat
    10261 tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt
    10321 gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc
    10381 cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc
    10441 gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat
    10501 tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct
    10561 tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat
    10621 gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct
    10681 ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct
    10741 gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt
    10801 tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata tatatagttc
    10861 aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct
    10921 tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa aataataaaa
    10981 aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat
    11041 atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa
    11101 aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa
    11161 gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta
    11221 gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat
    11281 agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat
    11341 ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa
    11401 aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat
    11461 tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc
    11521 tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa
    11581 gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt
    11641 gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacagt cgtagaagaa
    11701 ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca
    11761 gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc
    11821 gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta
    11881 ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat gagacattat
    11941 gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct
    12001 caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt
    12061 tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta
    12121 tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat
    12181 gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg
    12241 tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt
    12301 ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc
    12361 atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg tacgatgatt
    12421 tttttccaag cagattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt
    12481 agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca
    12541 gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata
    12601 acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc ttatttgacc
    12661 tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt cagtctaatc
    12721 ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc
    12781 gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc
    12841 tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca
    12901 tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag
    12961 atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc
    13021 ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa
    13081 acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg
    13141 accaatatgt tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca
    13201 ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt
    13261 cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt
    13321 ctattcggat ggcttcctcg aatgggtaaa cttgcttata tctacttagc ttactccttt
    13381 gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc
    13441 cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta
    13501 tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta
    13561 ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa
    13621 atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga
    13681 tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc
    13741 tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca
    13801 ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt
    13861 cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa
    13921 cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat
    13981 ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat
    14041 ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa
    14101 gctgttcctt tccgctagca gataattgat acactttcat actgccggag gccacgatca
    14161 ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt
    14221 tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa
    14281 atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa
    14341 taaagaaaca taacttccct tattccaact gataattttc ttttatcata gcataaacgt
    14401 caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc
    14461 ttcttttgag accttaagta attgattcct atcaattttt taaatattta ctttttgaat
    14521 aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat
    14581 ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt
    14641 gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt
    14701 tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat
    14761 cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata
    14821 tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt
    14881 tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga
    14941 tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt
    15001 cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga
    15061 ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct
    15121 ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg
    15181 gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg
    15241 attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt
    15301 gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc
    15361 aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac
    15421 atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat
    15481 tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc
    15541 ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg
    15601 cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc
    15661 cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc
    15721 ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc
    15781 gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg
    15841 atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc
    15901 tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc
    15961 tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt
    16021 ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca
    16081 ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat
    16141 aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac
    16201 atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac
    16261 acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc
    16321 cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac
    16381 accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt
    16441 tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc
    16501 tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag
    16561 gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc
    16621 atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat
    16681 atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact
    16741 atccgcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc
    16801 aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc
    16861 gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg
    16921 ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc
    16981 ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc
    17041 cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg
    17101 ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct
    17161 cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa
    17221 aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt
    17281 ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt
    17341 gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca
    17401 aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg
    17461 ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa gatatgtgta
    17521 gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac
    17581 aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga
    17641 ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg
    17701 cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga
    17761 aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta
    17821 tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc
    17881 cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac atgtagtggc
    17941 actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca tcctctcttt
    18001 tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc gcccctgcta
    18061 aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga cttgttgatt
    18121 tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc actgattttt
    18181 ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag tcagcttttt
    18241 ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg attgcttgac
    18301 tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt tgagcaacct
    18361 gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga tctgtttgga
    18421 cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca cggtcatttc
    18481 ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct tctttcaatt
    18541 tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc aagtcttgca
    18601 aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct gaagatgaaa
    18661 tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt
    18721 tttcagcacc agaagagctt atagttgttg aaggttctgt ttgagcaggt tcagaagttt
    18781 ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc cagattaacg
    18841 gcttcattct ttttttcatc acttttccca tccttactac tttttaacta atttcttatt
    18901 ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg
    18961 catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat aacctaaaat
    19021 cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta tccagaacaa
    19081 aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat catctacaat
    19141 cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga acacaaaact
    19201 gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat gcccgattaa
    19261 tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac tcaatgtttg
    19321 atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca cttttttgaa
    19381 attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctttgtc gatatagcat
    19441 cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa cagataacca
    19501 gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat cttgtctgaa
    19561 tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac tgtacgcata
    19621 aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac ttgagaattc
    19681 aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat ttgcagtgac
    19741 cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa tcagtgcatc
    19801 tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg caaaggcaag
    19861 tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca cacgaagatt
    19921 agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg aaccatcgtc
    19981 tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact cgattgtatc
    20041 ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg gttcgacact
    20101 caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat aacaccagac
    20161 accaataaac catacaaatc cgcctaaaat cggataagaa acgagaaaaa atacataata
    20221 gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat ttcagttcca
    20281 ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc tcttcggaga
    20341 cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc gtatacactt
    20401 cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattcaaaa gatttattta
    20461 cttttttgta tagatttcga ttgttccacc tagtcaatac aaatgaggta atcaggaaac
    20521 tgaaaaaaat caatacaaaa aagccgatta agaaatacag cattttcttt tctgataaaa
    20581 aagcgtacaa ggaaaaatcc ttggctcttt ctggtctcgt taatgggaaa aagacccata
    20641 taaaaggaag aacaacgatc atccatccta aaagagccac taacgtttgc caaatcttta
    20701 accagtaatt accttttgaa aaatacttat ctttcaccaa aattgatgca acttcttctc
    20761 ttgttacttt tttcatatcc tcacctcacg tttttttgtt acgacgtagg agtttaccat
    20821 acatttcatt tcttttcaca taaagtgttc ctaaattttt cctaagaaaa agaacaaaac
    20881 atttctcatc agcaaatgaa aaatattttg ttctttttat cagttgttct ttttattttt
    20941 tatctcttaa ttatgttgct ctttccaata cgacaatgct tttcattcct ctagatatcg
    21001 ccgtgtacat ttgtctgcgt cttctttgct catctccatc gttttcaaat ggatataaga
    21061 ttacatgatc aaattccagt ccttttgcta gactcaaagg aatgatttgg cagttttcta
    21121 atccatatga atttttttta atcgattctt gtagcgtttg ggtttcctct tctgtttggg
    21181 tgataactgc cgctttttct ccttttagag aaaccaaaat ctcagctagt attttcaaca
    21241 attcttcaga ggatcgaaaa gagcggtatt cgggttcttt tccttgcggt cggatcgata
    21301 cgatgtcgat cttctcttga tccacagcat aggttttgaa aagctctgtg attgctccgc
    21361 tagaacggta actatttcta agatcatacc gatggatcgg cagatggtag tcgtcgaaac
    21421 aacgcataat atcagcaaaa gtagtggaag aattaaagat tgcctgattc tcgtcaccta
    21481 ctaaagtaaa gtgagttttt ggaaataatt cgatcaatag acctaattgt gccagggtat
    21541 aatcttgtac ttcatcaatc aatagatagc gaaatgctgg tacatttacc ttttcaacaa
    21601 gtaaatgacg aatagctaat ataatgactg cttcatcaac agtacgtgtg ggttgccaag
    21661 cataagctga gccatatcgt ttttcataaa ttctttcaaa caactgatgc tcatccaccc
    21721 atgccatttc ttcgatttgt ctggttattt ttcgatattt tttttgtaaa agtttccttg
    21781 cataagctac aatactttgt tccgaatcat cagaaatcaa tttaccgaat aattccaact
    21841 gcctatcttc tgtcaacgag agtacctgat tctgcacagc aggacttttt gcttgtttca
    21901 aaagatgatt cttccattcg ctcatcagtg cctttttcgt tgcttgaagc ctatcgatca
    21961 atttaggatg agcaggaact ttctcatata ttttttcgat cgttttttta gaaaggactg
    22021 tttttccatt tttagtaagt ccacggataa aattcggatg atctaaaaac aaaggatctg
    22081 attgttttag attatcgata aactttttgc tacgcaaacg ttctgtctgc tccgatacat
    22141 tttctcccgt gatacgtttg aaataagcct cttcaccttc tatttcctct gctgaaagct
    22201 gtgaaaccag ctgtatgatg gttagattca gtggatttct ttcacctaat gatggtagga
    22261 cattcgaaat atagtcgata aaccgatgat taggtgatag aatcagcaag tcatcggatg
    22321 tcatcgtatc tcgatataaa tagagcagat acgcaatacg ctgcatgata gcagaggttt
    22381 taccacttcc tgctacgcca tttaccaaga tattttgact tttcacatcc cgaatgattg
    22441 tgttttgttc cgattggatc gtagcagtga tagccttcat ttcattggtc tcatcttggc
    22501 ctaagacttc taataatacg tcatcttgaa tagctacaga cgtatcaaaa aacttgacca
    22561 aacgatctct ttccaaaaga aattgtcgtc tttctttaat aaccgtatcg attttatttt
    22621 gacgaaccgt ataagaagat ggaccaatca tgttattgta aaaaagttcg gcaattggtg
    22681 aacgccaatc ataaacaaga ttttcatctg catcatctgt gaaatttgct gtaccgatat
    22741 aaaaggattc ttttggctca ttatccagaa agtctacaac gacttttcca aagtagggac
    22801 ttttcaataa acgttcgatc tttgctagct gattttctgc gtttttctgt ttgatattca
    22861 tctgatcgat ttctctattt ttcatttcaa tcatcgaaaa agtatccagg ttatctaagt
    22921 aactgtcaaa attcaatcga agctcgccag ccatttcttt caacgactgg tttccttcta
    22981 tttttgccgt atccatcgat tcccgcagtt cgtttttttt atcaattagc ttttgataca
    23041 cgttcttcag atatgcttct tctaactgtt tttgtgtgtt gaccatacac attctcctct
    23101 ttttttaaag tggtggaacc gattagttta cctcttttaa gagtcgaatg caagcttttt
    23161 aacgagtatc atttgttagg atatatcagt tcttcaggtc ggacagtttg atataaagaa
    23221 aatgcaccat caaggttttt tacgtggtag ccagactgct tcaagattct ttccgctaga
    23281 tagctacgta atccgctatg acagctgacg atatattctt gttggctgtc taattcattt
    23341 aatcggtccc gaagttcatt caaagggata tgtttggctt ccttgaattt gcccttttcc
    23401 aattcttctg ctgttcgcac atctaataaa atttttcctt ttgctagttc tgtcggcaat
    23461 tcatgccatt gtacggtttc actaagccct tctgcaatgt tcattgctgc atatcctagc
    23521 atgtttactg gatctttggc tgagccaaat ggcggtgcat acgtcaattc taattccggt
    23581 aaatcgaaga tcgtcaaatg ccctttgatt gctgttgcta aaatatcgat ccgtttatcc
    23641 acaccttttg ccccaacacc ttgtgcacca taaatctcgc cagtctctgg atggaagatc
    23701 aacttcagta aaatatctgt tgcttctgga taatagcttg catgatcttt tcctgataca
    23761 tgaatgactt caaaagacag ttcgttttct ttggctgttc tttcacttaa accggtggat
    23821 gcggcagtca ttccaaacgt tcgtataatt gctgttccaa taccgccttg atttcttcga
    23881 gaaattccgg aaatcgtgtc cgcaacttgt ctaccttgac gattggctgg tgaagcaaga
    23941 gaaataagtg catcttttcc agtgatttgc tgttttacaa cgatagcatc ccccaccgca
    24001 taaatatcag gaatattcgt ttgataacgt tcgtctacta ggataccccc gcgtatgcct
    24061 aattcgattc cagcggtcac tgctagctgg ttatcaggac gaacaccaat ggataaaata
    24121 actaaatcag cagggatggt acgcccatca tccagtcgca atcgtttccc gtggtcttca
    24181 aatccagcga cagcatgaga agtaatcact tctactcctt gatgagacaa ttctttttct
    24241 ataaaagctg ccatttcttc atccagtgtt ggaagaatat gtggtgcttg ttcgatcacc
    24301 atgacttcta aacctcttct ttttaggttt tctgccattt ccaatccgat gaatcctgca
    24361 ccaacgatca cggcacgctt tggctgtttt tctaatgcat gcatcacttt atcgatatct
    24421 ggtacatttc taatagaaaa aacattgtcg gcttctgtta tccctggaat cgatggaaca
    24481 actggttttg caccaggaga taaaaccaat gcatcgtact gttcaatgtg tttttgaccc
    24541 tcatgtacga cttctaccgt tttgttttcg ggaaagatgg ctgtcacttc atgatttgga
    24601 cgcacatcta aacaaaaacg agcggataat gattctggcg tttggacaag aagattttcc
    24661 cgttcactga tttctcccga aagataataa ggcaagccgc aatttgcaaa agaaacatac
    24721 ggtccttttt caaaaacaac gatttctgca tcttccatta atcggcgaag ccgtgtagca
    24781 gcagacattc cacctgctac acctccgaca ataacgattt tcatcttatt tccctcctac
    24841 tgttcgatca aaccattgat tcatgccacc acgtacattc actacatcat aaccagattt
    24901 ctttaattct ttagctgctt gtttactcct catccctgat tgacaaatca catagacggt
    24961 cttttctttg tctccatcat atctgtttat actttgtaat ggaacatttt tagctccttt
    25021 tatatgcccg ccccggtatt ctgcaggtgt tctaacatca agtaacgctg gattttccaa
    25081 aagcttttcc tttaattctg ggactgttat cgattttatt gattgaaata gcaaattcat
    25141 tttctgcacc tcatttacat ttttataaat acctcatggg gtatattaaa caggagcttt
    25201 attaatatgt caaccagaat gactcttcat ttttttcact actttttata tttgcataaa
    25261 ataccgataa ggggtattat ctaattataa aaatagagga gaatgtgtta tggagattga
    25321 taataaaaat atcctgaacc gtttgaaacg tacagaggga caaattcgtg gtatccaaaa
    25381 aatgatcgaa gacgaaaaag aatgtatgga tgtcatcact caattaagcg ctgttcgttc
    25441 tagtattgat cgcgtaatgg gcatgatcgt tgcagataat ctgaaaaatt gctttgagaa
    25501 acctgatagt aatccagaag aacaagcggc taaactagaa caagcgatca agatgattat
    25561 taaaaagtaa aggaatgatt tcaatgaaag tcgttttcca tattgatgaa ttagaaaaat
    25621 ggtcagaaac agggaaaaac gtaaaaaacc tgataaaagc ttctccagaa acgacgattg
    25681 tcgtatctgt aaatggcatc gcgattaccg gttatctaga ctctgccaat gcagaattct
    25741 tagatttgca aggagtcgtt tttcacgctt gcgccaatgc tatgcgtgct aatcatattt
    25801 cagaaagttc tttacctgaa caagtaatcg ttgtaccagc aggtgtcctt gatttagttg
    25861 aactgcaatc ccaaggttat gcttatatca agccttagtc agtagaggtt gcgacagaag
    25921 tgatcagctt caagaaattt cggcttattt ttcgaagaag ctacttctgg aaaaccgtga
    25981 atcaaaggtt gtgaaaacgc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    26041 cttctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    26101 ttaaggaaga ggttgtgaaa tcagcggttt gacctaagta ttagagaaaa aaacgggaaa
    26161 atagctcctc atattttgcc gttttttgag ctaataccga aggtcagctg attgaacacc
    26221 gtttaaggaa gagattgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
    26281 aaatggattt ctacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaat
    26341 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gaaacaaacg
    26401 gaaaaatagc tgctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
    26461 acaccgttta ccaagaggtt gtgagcctac catttaactc cgaatcacaa gaaataattt
    26521 caaaaacaac ttctcctgtt tttggtttgg ttggcttatt atcgagaagt agctactggg
    26581 agtctattta tcaaaaaaag gaatgcgaca taggtcacac ccctttttta ttttctttga
    26641 aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc gtaagtgatg
    26701 acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag caaaaggatc
    26761 atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc acttaatttg
    26821 atatttttag atagcccgat taatccagct acaaacataa agccttcttt tatcaagaat
    26881 agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat cagaaaaagt
    26941 tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac ctgattatat
    27001 ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat gaccgctcct
    27061 gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa aaaaatacgg
    27121 aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt gcttaatttc
    27181 attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac acggggaata
    27241 aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat gagctaattt
    27301 gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt ttgcgcattt
    27361 cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc tgccgtttac
    27421 gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac agatcttctg
    27481 ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg tctaaatata
    27541 catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg tattcattga
    27601 caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc ttcctatttg
    27661 taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt tccgccaagt
    27721 aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca atccacaaag
    27781 cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt gcttcccgat
    27841 cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc ttgatttctc
    27901 cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc atgattgcga
    27961 tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct aaaagtacca
    28021 acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga ccagaaaatg
    28081 tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc tcatcaaaga
    28141 cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc atcaaagtca
    28201 ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg gtgataatca
    28261 agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca ggtaaaaatg
    28321 gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt gtgcttccat
    28381 tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg atcgtttcga
    28441 tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc gccaggactt
    28501 gatattcttc actgatttct tcagctttta acaactgata gtgttcattc aatttagaaa
    28561 tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata atcgttccat
    28621 ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct ttttgaacaa
    28681 tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc agtaattttg
    28741 actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa ttacgtgacg
    28801 cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct acatttatca
    28861 aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga ttcttatcaa
    28921 aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc aaatcggttg
    28981 gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc ggcaacccca
    29041 taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt tcttcttgac
    29101 gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt tttttgctat
    29161 agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt aattgactaa
    29221 tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca aaataaataa
    29281 gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc ctatccttat
    29341 tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac caaataaact
    29401 ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa aacatacccc
    29461 attcttctag gtcttattta ttttccactt aactatagca agacacattt tggttgtcaa
    29521 actttagtat gttttataga tttacaccat taatgaatag acacctaccg cgataattgc
    29581 tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag ttcctttatt
    29641 agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag ggttgattgc
    29701 atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac ctacagcgaa
    29761 tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa gaactaatac
    29821 aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag caggacctgt
    29881 tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg gcaaataaga
    29941 aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaatga tgaaaggaac
    30001 gacaagattc caactaattg cccctgtcat cgccatagct agagagactg caggattcaa
    30061 atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga gtccccaacc
    30121 aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat tcacagccgc
    30181 acacacacca tctccaagca aaactaggat cagtgtccca ataaactctc ccatgatttg
    30241 agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg attctgcaat
    30301 caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt caaaatatcc
    30361 agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt caaacaacag
    30421 atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt cttctaaacc
    30481 ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca tttcttttgc
    30541 taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag tagcatcttc
    30601 ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt cttctaattt
    30661 agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt ctttcgtctc
    30721 tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt ctgccatctt
    30781 acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt ctctttctaa
    30841 agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt ccaaatggtt
    30901 caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac ttactgtatc
    30961 gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat tgtaatcaga
    31021 accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga tatcattcaa
    31081 tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat ctacatcttc
    31141 ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg taccaaagta
    31201 agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc ctgtatcgaa
    31261 gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga tccctttagt
    31321 tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt tatctaccca
    31381 aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat ctgtcagcag
    31441 gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa ccgctttcat
    31501 tttacttact agataagccc catcttcggc agcctttttg atattatcaa tcactaaacg
    31561 ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt cttttttcaa
    31621 aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat atttggttcc
    31681 tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga acatattgaa
    31741 ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt ttgggatgtg
    31801 tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta cttccacatc
    31861 aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag aggaagtccc
    31921 ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg ctgactggac
    31981 tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt cttcttgctg
    32041 cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt cctccttatt
    32101 caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt tctcggcgct
    32161 cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg atctcgtctg
    32221 tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc gccgttgttt
    32281 ccaagttttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat tgcatcagat
    32341 attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt ccagtgtcat
    32401 cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa gtagctttaa
    32461 tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt gctgcctgat
    32521 cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca tcttcgcttg
    32581 tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga agtccatctc
    32641 tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca tagtaaacct
    32701 ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat tgcggttttt
    32761 caccggtatt catgacaata aatgaacctg taccataagt attcttgacc atgcctggtt
    32821 cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct gaaattggtg
    32881 cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag ttagaagtga
    32941 cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga tcccaatcta
    33001 gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta acatgtgctt
    33061 gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc aattcgcctt
    33121 tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc ttcgttgctg
    33181 agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga tagccttcat
    33241 tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact gcatgataga
    33301 ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc gttatgccaa
    33361 tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca ataactgact
    33421 gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc gggaaatatt
    33481 gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag ataattgctc
    33541 gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc attgtgattc
    33601 cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt tttaaaaaaa
    33661 gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt atgaagctat
    33721 tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat cgaataatgt
    33781 cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt ctctggcaaa
    33841 atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca ataccgccga
    33901 ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc ggcagacttt
    33961 ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc aaaagaacat
    34021 tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc ctcggcaatt
    34081 gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc ccgagcattt
    34141 tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca acaaagaaaa
    34201 accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct ttgattttca
    34261 aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct aaaacagcgg
    34321 ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg tctactatcg
    34381 aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta tacattcagt tctgctgctg
    34441 ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg tccgactgat
    34501 agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat agtctttttt
    34561 tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac gttgtttcta
    34621 atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc aaaaaaagcg
    34681 gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt atctgtagct
    34741 cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag agctggttca
    34801 attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc gctaatcgaa
    34861 cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc aagctttcct
    34921 gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca agcagcgcct
    34981 gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc aattcaatat
    35041 cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc aaaccattga
    35101 tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt aactgctctt
    35161 ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga ccacaccata
    35221 tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg ttcaaacgtc
    35281 catccttctt ctactgcaat tgtcagttgg tcaagaaggt ggaacatctc ttttttgctt
    35341 tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag tttaactttg
    35401 ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt ttggggataa
    35461 aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa ccactgattg
    35521 ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt cttgctgaca
    35581 tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc atttgttgga
    35641 cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga cgtttgcaag
    35701 tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc ccacatctga
    35761 ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt tcttttttcc
    35821 gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc taaaaaaagt
    35881 gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg atctagataa
    35941 cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct tgatgccaac
    36001 ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt ctttgcattc
    36061 atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg aataagcgtt
    36121 gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat caaacgatca
    36181 aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc tattactgtc
    36241 ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt gatccaagaa
    36301 agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct gggaatccat
    36361 cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa ttggcgaaga
    36421 taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt catctgcatt
    36481 cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca gtattcataa
    36541 ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc catcataccg
    36601 tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt atcatttctg
    36661 aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt tacgtacctc
    36721 tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt aatgtaatag
    36781 ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg cttcgcctaa
    36841 ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt gaaccacttc
    36901 tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat cttctgctaa
    36961 gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga acccagtcac
    37021 tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca atcctacaac
    37081 accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag cgactttccc
    37141 aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat aataaaccgc
    37201 tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg cccgatttct
    37261 gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa cactttgcgg
    37321 ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc cattttttac
    37381 taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac gctcgatctt
    37441 ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat cctctttcta
    37501 taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct tggcaaataa
    37561 agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg gttttccaat
    37621 aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc aagttctgca
    37681 tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc aactaaaggg
    37741 atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt gtatttatca
    37801 gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag ttttgactca
    37861 cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt ttcatattct
    37921 tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc aaccgctgag
    37981 aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct accgccccat
    38041 tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata cgtatttgct
    38101 ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc tttgccaaat
    38161 gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc cgcatcagga
    38221 cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt tgctcgactt
    38281 gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc ttggattgct
    38341 ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag ttcacctggc
    38401 cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc aagaccttta
    38461 gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa aaagctgagt
    38521 tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga aggtaaagta
    38581 attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga ttaacctatc
    38641 gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt tgcttaaaaa
    38701 attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta attctttact
    38761 tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc taccatcgac
    38821 ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc gaccttcgct
    38881 aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa tcgttgatgg
    38941 ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt cttttaatat
    39001 caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt catcagctaa
    39061 tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat ctaacgcttt
    39121 ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt atgattttta
    39181 ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg aaaaaaagaa
    39241 gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg aacaccgtga
    39301 atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt gtgaaaatgg
    39361 ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt gaacaccgtt
    39421 tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc caaacaaaaa
    39481 tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga ttgtacaccg
    39541 tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca atttcaaaaa
    39601 acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc aggagaatac
    39661 tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt atttcctttt
    39721 attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat gattttttct
    39781 ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata tatccataac
    39841 gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac gacgtataac
    39901 caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc tgtttcaaat
    39961 agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct atagctccga
    40021 gtccgttttc agctatgaac agtggttttt gatacctatc atataattga ttcaaagcaa
    40081 ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga tacggatttt
    40141 taattcctcc agttattaag tttccttgga ccccttccca attttctgga tgagcactga
    40201 ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct gctaagattt
    40261 cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt cgttgcgtat
    40321 acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt ctttgctgct
    40381 caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc tgatagctga
    40441 tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta gctaaagcac
    40501 ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg ggatggtttt
    40561 cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc tcattgaaag
    40621 tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg aaacgaacgt
    40681 aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa tataacggca
    40741 tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat tcgtcaaaca
    40801 cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca tgaggaaaaa
    40861 ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca aataaacgga
    40921 tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa taatacttgc
    40981 tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat tgatctgtcc
    41041 atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat aatccttttt
    41101 catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc cataaaaatc
    41161 cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg tttgattatt
    41221 tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa taaccattgt
    41281 ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca ataaatcgcc
    41341 tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat ttaaattcac
    41401 tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta ccccaattgc
    41461 atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca ggactgtaga tttttccgtc
    41521 cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg gatcgtttac
    41581 ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat cctttgagga
    41641 ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg aactgatcat
    41701 cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa tcgggaaaga
    41761 caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag ctactccacc
    41821 aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa gagtcacacc
    41881 ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata aagataaact
    41941 ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg cttgcgcagc
    42001 tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa gattgttggt
    42061 cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc aaaatccgcc
    42121 gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc cagctgcaat
    42181 cagctgactt gcataatctg caattggacc tacaactaat aaagtaattg ggaccatcac
    42241 gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc ttttgcgaat
    42301 cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa ccgtacttgt
    42361 ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat cactatatgc
    42421 actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga aacgatctgt
    42481 attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga aaaatgcatc
    42541 agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat tcaaactcgt
    42601 acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta aagtcggggc
    42661 aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct cagttcctct
    42721 ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt catacacttc
    42781 atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta cagtgatcac
    42841 accttctagt tgctctacct tctccttatc tgccttctta ttatctttta gggtaaagcg
    42901 taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga caaatcggac
    42961 aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa ttaatgccgt
    43021 agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca ttcaattagt
    43081 accccttata atagcgaacg aaactgtaca atagtgtgta tggtttactt agccgagcaa
    43141 atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa aggagacaaa
    43201 aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct gatactctgt
    43261 gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg catttaacgg
    43321 aagcataact aggtcagaac taggagagat tagttgctga atggaatcta tctttttatg
    43381 gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt tctttttttc
    43441 ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt caaactcatc
    43501 tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt gctcagtagt
    43561 caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat acactggttc
    43621 tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt ttagtgtatt
    43681 caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc gaaaaggatt
    43741 caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg gacgtaattg
    43801 aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac gggagatgat
    43861 cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt tcatttgttt
    43921 gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa gatctaaaaa
    43981 acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt ttttttcatc
    44041 attaatcatc aaataattta actctaatag tcgattttca attgcttgtt tttcttgatt
    44101 ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta ccatcggaat
    44161 aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa tggtcaaaac
    44221 gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat atgatataag
    44281 aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg tactgatttg
    44341 ttgaggcaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata agaaaattct
    44401 ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt ccagttctaa
    44461 tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata cggctgtttt
    44521 tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt gaaacaataa
    44581 gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat gtttcgtcac
    44641 ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat aacctgaacg
    44701 tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt tgatttcagt
    44761 ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg ttatccaatg
    44821 attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt ccatttgctt
    44881 acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat agtcacttgc
    44941 agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca ttagactgga
    45001 taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt ccgctcccat
    45061 cattttgtct atccacataa ataaagccat atcgcttcct catttcccct gttcctgccg
    45121 atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca tcttttattg
    45181 cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga tcgtggattc
    45241 gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg aataatggta
    45301 attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga tcaattggcc
    45361 aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca tttgctccgc
    45421 cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa taactaaatg
    45481 caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac attactggtg
    45541 cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct cggcacatga
    45601 catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca ttctctggat
    45661 cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc atcttagcat
    45721 ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac atatgatgac
    45781 aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta tctctagcag
    45841 ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg atctcattaa
    45901 aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc gcataaaatt
    45961 caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc aatgctaagg
    46021 gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt aatgtattga
    46081 ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct cctcttggaa
    46141 atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc ttcagtaaac
    46201 gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga taatggagtg
    46261 attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata ctaggatgct
    46321 ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct tccaaccatg
    46381 caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct ggaaatattt
    46441 cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt atagctcgat
    46501 tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat tgaatgctac
    46561 tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa agaaaacact
    46621 cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc ataaggtcgt
    46681 aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat aacccttaaa
    46741 gcttcacgtg gaacagtgaa cgtctgcttt tcactggaca tattacaaat aacgagccat
    46801 tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt ttccactaat
    46861 tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa tttcttgtac
    46921 atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc tttataattt
    46981 ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg ggcgttccat
    47041 tgcataggcg tcctagcatt gtctcttcct ttcgcattga ttgattccaa gatttcctct
    47101 tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat atcttttgct
    47161 tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg atagatataa
    47221 ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc ttttcgatac
    47281 acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt ccaaaataaa
    47341 ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt tttcagttca
    47401 gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc taatcctatg
    47461 tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata aagtttagcg
    47521 atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc accaaaagtt
    47581 gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt gatttcttta
    47641 tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat acctttatcg
    47701 atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt ccaattcaaa
    47761 tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc atccaactgc
    47821 caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt gaccggatca
    47881 cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc aatgaaccac
    47941 ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat atttcttttc
    48001 tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc catgattgct
    48061 tgataattac tgatatcata cccattgtca tcattcggag actcgtatac tggagaaagc
    48121 caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat cccccgcaaa
    48181 tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg ataaacaact
    48241 gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt ttctattttt
    48301 tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca aatagctatt
    48361 gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt ccctcaactg
    48421 ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt ctttataaac
    48481 ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat gaggaactat
    48541 tttttcgttt ttttctctaa tactcaggcc agaacactga tttcacaacc tctttataaa
    48601 cggtgttcac tcagctgtcc ctcggtatta gctcaaaaaa cggcaaaata taagaaacta
    48661 ttttttcgtt tttttctcta atactcaggc cagaacgctg atttcacaac ctctttattt
    48721 cacattcaat atccacatga atcccacaaa tacaaagaac agaccataca tgattggaga
    48781 tacttctttt cctcttttag ctgctaacat ggtaattgga tagaagataa aacctaatgc
    48841 aatcccatct gaaatactat aagtcaaagg catgccgatc aagatcaaga atgaagggat
    48901 cgcaatctct aagtttcccc attcgatttg tcgcaaagat tgtgccatca atactcctac
    48961 cataatcaat gctggtgcag ttacttgtga agtaacaact gatagtaatg gagaaaagaa
    49021 caagcctaac aagaagaaaa tccctgtagt aatagctgtg atacctgaac gtcctccaac
    49081 ggcgatacca gctgatgatt ctacaaatgc accaacagga gaagtaccaa ataaggaacc
    49141 cgcaagcata gctgttgaat cagaagctaa agctttccct acacgaggca tcttgttgtc
    49201 tttcatgaaa cctgcttggt ttgctaatcc aactaaagtt cctgctgtat caaagaaagt
    49261 cacaagcaag aatgtcagca ctacgaccca catttgaaga gaattgatgt cccctacatg
    49321 ttttaatgca actaaaaatg ttggtttcaa acttggtgct gcggcaacaa tttcagaagg
    49381 cattggaatc aaacctgtga ccagacctaa aatcgttgta gctgctatcc cgatgaagat
    49441 gccacctggt acacgccgaa caagcaagat agcagttacg actaatccaa aaatcgtcaa
    49501 ccaagtagaa ccaacgttga gtgatcctaa tcctactaat gtagattcat tcgaaacgat
    49561 gatcccacct tcgctcagac caaggaaagc gataaataga ccgataccgc cagaaatcgc
    49621 atatttcaaa tctgctggga tcgcatcaat gatcatttcc cgcagcttga agattgtgat
    49681 caaaataaaa atcaaagaag caacgaatac gccggctaat gctgtttccc atggaatccc
    49741 cattcctaaa caaacagaat aagcaaaaaa agcattgatc ccaagagcag gtgccgttcc
    49801 aattggataa cgggctaata cccccattaa aacacagccg atcgcactgg ctaatgcagt
    49861 cgcagtaaac actgctcctt catccattcc agaagcacct aatacagttg gatttacaaa
    49921 taagatataa gccattgaga taaatgttgt aaatcctgcc agtacctcac gttttaccgt
    49981 ggtgttcaat ttttctattt cgaagtagga aagtattttt tccttcatct tccatccccc
    50041 taaatccgaa cattataaat aaagtccgtt aaaatttcga tttattaata aaacaacgtg
    50101 ttttatcata tactaaaaca aaacataagt aaacaaaaaa ttcggatttt aagcaaaatc
    50161 cgaacgttcc atctgattaa atccctttat ctcaaagttc atgaacattc actttggatt
    50221 acaagaattt ctttattgtg ttaataaagt ttcttcattc tagtctcaaa aatcaatagt
    50281 gtgatttgat tttaccattt cgtttctgtt cttctttgat gaattctttt gtatcgacag
    50341 gattcagatt atttagtccg ccaaaacctg tatcgaccat ttttccccag ccttttgtct
    50401 tcttttttcc tgatttgaga ttctttgctc gaagcttctc actatgtttt gctcgccgat
    50461 acttcaaata accttctaat cttcccatta gatccactgt ttttcctcct ttttattcaa
    50521 tcagacgact cagcttttcc tataacaaaa taattgcctt cagggtcaga aaaattgaaa
    50581 gtcaagatgc cattgttatc tgtgatttct cctggttctt ccatgttctc gtagatagtt
    50641 tcaaattcat ccgtaaaaaa tactaaagaa ggcggtggtc ctaatacctc tggtgaaaac
    50701 ttctgcacaa atacttttgg gaatacgcct aattctaatt cttttgagat gctgagtaca
    50761 atactcttaa attcttctgg caattcgatt gtttcagaaa tttctgcacc aattttttct
    50821 acaaagaatc ggcaaatcat ctctacgtca ttgacataaa gcatcacacg tgttttttgt
    50881 atcatcattt gacccttctt tctatttgat tttctttcct gtctataaac tgattccatt
    50941 atatcacgaa agcgcaaaca ccttgtgttt gatactattc aagaaaaata aagcaatagt
    51001 ttctaggatt gctttcctcc acaaactttt tttacaaaaa aaaaaaacgg caatgaatgc
    51061 cgctcttctt atttcgattc ttttataatt ctgcttctga ttcatctttg gttagtgtgt
    51121 gtctaacact agttacgcca aatgtttgat cgattttttc cttcaacgct tcgattgcct
    51181 gtgcgccacg tgccgcttgt tcagcattga tggattcaat gaccagcacc gctgcttgtg
    51241 tttcttccgt cagcattgtt ctttgggctt cacgccagtt gagacagcga cagatcgctc
    51301 cttgttcatc atagtagatg atttcttccg gaagtgccgg agcatcggaa tctgcaccta
    51361 aaggcaggaa tgattcgcca ccttgggctt ttcctaaatg cagatttcct gcgatctttt
    51421 cgatatcttc tccgccgcaa ggtacgccaa aagacaagga aatactatta tagatatcta
    51481 caagcggatt gattgggaaa aattcccgtt cttgatttgc acgtttcagt aaagcttcaa
    51541 tagaagaacg tgcacctttt ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa
    51601 tgacttcgtt ttggctgaat gtttcttctg taaggtaatt tcttgcttct tttgctgcct
    51661 cattcaataa ttgatgatac gtttcttcac tttcttttgt tacgtggtta tcgataccat
    51721 ttacggataa gatttcgatg gttgcttcag gaaatacttc ccagaaattg gtatccacta
    51781 ttactttttt catctgttca cacctcttga ttagaataat tgcttgatga atagatagtc
    51841 tgtttgggga tcatctccca tataaaaaac atgttcgctg tatttgataa atcctttttt
    51901 acgataaaaa gcgagcgcaa catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt
    51961 ttgcttttct tttgcttttt caatagctgt atctagtaaa aatgtaccga taccttgatg
    52021 tttaaatttt tttcgtacat agattcgctc gatttctaaa aaatgatcag ccatttcctc
    52081 agattgactc gttcctgtat ttagtttcag gtaacccgca ggctcttgat cgatactcac
    52141 taggaaaaaa tcggtttctt tatcttttag ctcttctgta agtttttcta gtgcataagc
    52201 ttgtgtcaag taatcagcta aatgctccgg tgaattatct tttccaaatg tatctgtaaa
    52261 tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc
    52321 gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt tgacaaaatt
    52381 ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt gttttgcttc
    52441 ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc gaatgatgaa
    52501 aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt tattttcttt
    52561 atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc gggcagtcgt
    52621 tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag gattttcgca
    52681 aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga attcgatatt
    52741 gctaatagaa tctagcgctc gggcaatcat tccgatgtct cgaaggattt cagccatttc
    52801 atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa atgcaactat
    52861 tttcaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct tttcttcttt
    52921 tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag taactgtttc
    52981 ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa tcccatgttg
    53041 gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca
    53101 agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc aaaaactgct
    53161 cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt acgcacattt
    53221 tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt
    53281 ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac atctactttt
    53341 ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa agtttctttt
    53401 cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg ctctttatca
    53461 aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct tttgtattga
    53521 gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact atgtaatcaa
    53581 gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa taaataaaag
    53641 aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg aataaatggc
    53701 gttcaaaagc ttgcatctgc ggtaataagc cccaccaaac gaaaaatatg acgagctatt
    53761 cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct ccccactaaa
    53821 cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac atgagaagct
    53881 gtttttttga aattgctcct tgtgactcgg agctgaacga caggctcacg acctctcggt
    53941 aaacagcgtt caaaagccaa tttttcgata ttaagccatt tctttgttac ttattgtcat
    54001 tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta tagtctgtta
    54061 catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg gcgatgatct
    54121 ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc aatacgtcac
    54181 catagtgata atgatccatg atatagctca aatgacgatc tgattcacga tcacgtttgt
    54241 accgacgttg acataacact tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc
    54301 cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa ggatactcgg
    54361 acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa tagaaaggat
    54421 aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg atggattctg
    54481 catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat cgttcccgta
    54541 gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga atgaaatact
    54601 ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa cagtccaatc
    54661 ttatgcttga caatttcttt tactgcttca tttgctggaa tcacattatg gcgaagagac
    54721 ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt gactaaaagc
    54781 tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata atcttcttct
    54841 ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc gatttctttc
    54901 agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt accgatagca
    54961 atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc cggtcttgta
    55021 tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat tgttccaagt
    55081 tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg
    55141 atattttctt tgaaaggcag atgggaccca tcatacatga cagaagtata tccgctgtcg
    55201 attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc
    55261 atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc gatatattta
    55321 gcggtatcga cacttgtttg aataatgatc ggcgaaccca tttcttttgc tgcgcctagc
    55381 atagcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa attcaactct
    55441 tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca ccttatcttt
    55501 ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat ccattttgcc
    55561 catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac gcttctggtt
    55621 atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt ccaaactctt
    55681 ttctaaatat tggatggctt gatcgttcac atacaattca gaaaatttaa gtccaacttg
    55741 ttcataaagt ttcgctaatt tatgcttatc ttgggtagtg gaaatctctt gtttcaattg
    55801 tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt cttcttttgc
    55861 cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca ttttccctac
    55921 tttcaattaa aatgctttca aaactgggct taagatccag gcatataaga gtgctgcggc
    55981 tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa agatcgttac
    56041 tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa tgatcgctcc
    56101 tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa agaaattggt
    56161 tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca tggcaataat
    56221 cgtaccaatc gttgtcgtaa tgaaacctaa aatgacagca tttgggctat aaggaaagaa
    56281 aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta tccctcgaaa
    56341 tgccggcacg atttcaccta acagtagacg tacaccagct agcaatacat acacacccac
    56401 tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc ctgataattc
    56461 agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga ccatcacgac
    56521 catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc
    56581 gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg cttcaaataa
    56641 gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga ttttacgaat
    56701 gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa aaccaccgac
    56761 caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg tcatcgttgc
    56821 catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga aacgagccaa
    56881 aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac ctaaacccaa
    56941 atcattcatt gcttgaccat tgatagattc aatagaaggg atgatccctt gcatatgaaa
    57001 tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc tcgaaccggc
    57061 actcaacact tgaaatccta ggagtgtctt taacgtgcca gagatgactt gtcctgctga
    57121 tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg atgcctgagt
    57181 caaaatattt tcgatgataa aattgataac gcccatgttt ttaccctctt ttctttttgt
    57241 atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat ttcatttttt
    57301 gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa cttttcgaac
    57361 tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac tgcggaagaa
    57421 atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc ctcagcagac
    57481 atctgtgctg caaaactact tcctaaacct gcaccgcata cgaataaaat gttcaatttg
    57541 ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa aagcagttgt
    57601 ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc gttgttgatc
    57661 aattcaatta gttctttcat gatgttcaga tgcgagtagg agtctactgc tgccaaacaa
    57721 aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg ctcaatcgta
    57781 gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc tagtgccatg
    57841 tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat cgcttcgaca
    57901 tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc aatcgattcc
    57961 tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat atcttttaac
    58021 ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa tttttctaac
    58081 ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc tattatttga
    58141 gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc ttcatttctt
    58201 ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc gacaactaaa
    58261 agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac atccaattta
    58321 tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg aggaaacttt
    58381 tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca gacaacgact
    58441 gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga aaagtggatg
    58501 acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc gatcgtagga
    58561 acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt ttctttcaat
    58621 ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat gtgcttatag
    58681 agattttcga ataaagcttc ttcctttaga tggaaaggta cgtgtgtctc gttctctaca
    58741 aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga attggagata
    58801 tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac ttgcgggaca
    58861 ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct actgtctttt
    58921 atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat tcgctggatc
    58981 cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg tttcaacaac
    59041 ttattggaat agatctcctg tatttttttg atttccacca aaggaaaata atggagaaag
    59101 attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt cattcccaga
    59161 ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc tttataaacc
    59221 agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt tgctcgtaac
    59281 cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc ttttttgttt
    59341 agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc
    59401 cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc ttgcttttcc
    59461 ttttccagct tcaacaaaaa aactttgttg cgaattgtac ggatctccgg aagcttttta
    59521 ctgctcaacc agatattcgc ttcaagaact tcctgacgga tcgtccgctg actgacattg
    59581 aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat tctgtgaagt
    59641 atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt atatggttcc
    59701 gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag taagcaaagc
    59761 aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat acgcctatta
    59821 ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa
    59881 gtaataagaa caactaaact aaaatagtgc ttcctatttt tcgtttggtt gttcttatta
    59941 tcgcagatgc aagcttttaa acaccattta gtggagaggt tgtgacagag atgttcagcc
    60001 ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta
    60061 ttgccgatag gctatctctg gcacactgtt tattcagaaa aaggagctgc gacaaggctt
    60121 ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggc tgaataaaat
    60181 cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga gaaaaatact
    60241 gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact gaataatcac
    60301 agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga taatttccta
    60361 cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg tttttttgca
    60421 gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct ttttcaggag
    60481 tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc agaaactgtg
    60541 ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgctttc agccttttct
    60601 tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac aaaacagcta
    60661 atgcaagaaa tcccgtttgc tcataactag aaggacctgt acctggacca taatacccat
    60721 aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa cgaccaccaa
    60781 atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca cccgcgacag
    60841 cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg attgctaaat
    60901 aattgatttt tttcattgtc ttcctcgtta tcttttatta caaaaaacta tttttatgat
    60961 agccttgcta aaaggaagag tcaataacca gatgcataaa ctgttttttt agcaattttg
    61021 tcttatttag tcattgagta acatcggaca aatgtatgtg tcggcagtcc ataattcctc
    61081 tatcttcagc atgaacaagc ttgcagacaa gagatctgcg cttcctccag gagagatttg
    61141 gttttcctga caaaagtgca acagtgctgt aaatggacga tttggtaaag cactttgact
    61201 agcagcatga gcaagttttt gcatttccac taacgttttc tgatcactgc gatgcaatac
    61261 gcatgtgtca tctagcgaac tcatcaaaac taacaacata tgcatttgtt tctgtacata
    61321 tgaatcatta tctagctcct tagctgcttg gattgcaagc ctgatatgcg gataccctaa
    61381 tatcgcttct tcatatgcac catttacgcc atatttttgc ttcaccctat gaccatgagt
    61441 ggttttttta tgacgatatt ttgtatcagg aaaagcagca agcttgctga catcatcaag
    61501 tattttcgat aaagaaaaag gctgttccat actgattttt tgtgcaatga cactgatcaa
    61561 tagccccatt gcccaaatcg ctcctttatg ggtattgatg ccatttgtag caatcagcat
    61621 tgccttttcc gcttttcgcc caatctcagc aattctttct cttaactcct gatcaatagc
    61681 atgatgccat gctatttgag ccatttcttc aaaaaaagga gttagcgtct tagcagactt
    61741 aagaaataaa gcatagtcca tatcggaatg tgcacccgca tccagtgcat ccactaatcc
    61801 aggttttgga cttaatgtta cttctgcgat caatgcttcc tctaccaacg cagcgagttg
    61861 ctgagtcact gctttttttc gttcgtcatt tatcatgaca ctcactcctt cttcctacta
    61921 ttacttgctg aaaccttttt tgatagctac ccaaatgata tgaagtaacc agcttaaagc
    61981 aaatgcacaa cttgccgtca ttagataagc tacaagaatc atgactcctg aaggtaactg
    62041 cagccaacta tctttaaaca ataataacag tacttggaaa acaaatacat tggctaaata
    62101 cgtacgataa gcataaacag acaaccattt gaacaatggc aattttggcg aatcgtttac
    62161 aatcattttg gaagcgccta tgaagacagc aaagatgatg actaaactgt ataaactttg
    62221 gatcgtattc aagtaaggtg cattcgcaaa gctcaagtct cctggatgtg cgagcaatga
    62281 acggacagaa aagaacgcga taactaaggc aacaggtaag aaaagataac gtactctgtc
    62341 caaatattga tagacttttt cgtgataaac aaatgcggct accccataaa tcatgaaact
    62401 agcaacaaac cgatctaata agtaccagct gtcatgataa ggcccagtca atacttgtgt
    62461 ttgatagaac agataccatg cgacatataa aatcgttgaa ccaatcaata ctggccatac
    62521 atatttttta ttagcaaata ctttgtaacc gagccaaaca aaaaacggca tgaacaattg
    62581 gatctgcagc atcataacgg tataccataa atgaggcgct ccatcacctg tgacatattt
    62641 cagtaaaaag cttgcagtat ccgtatacgg agtttgttgt tggactgctg gaaatagcca
    62701 aagataagcc gtcgtccata gaagatacgg aacgactagt tcaaaaaact tttctttcaa
    62761 aaattcccaa taagacaaat gctgacttgt ttttaccata ttataaacaa ttgcaaaaat
    62821 aaacattggt gctgtgtatt taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt
    62881 tgcgctgtgg ctgtttgcca aagcaaagct caatatcgtt tgcatcataa cagccgttac
    62941 agctgatact ttctggaagt cacttacgtt gattttgatt tttttgtttt caaacatctc
    63001 tttctctctc tttttcttta ttaccaaagt tctttggtta atttaggtcc ttcgactgtt
    63061 ttcaatagaa tttttttctc tggctctctc aaatactctt ttagtgaaaa accgccttgc
    63121 cgagtcacta cttgggtatc gaattgctca aaaaaagcag gatgcgattg aattacctga
    63181 attggcaatt caatcggtga atttgcgtac agcagcaaat caaaatcact ggtttctttt
    63241 actgccggaa tcccagtcga taattcaaat cctaagctgc cgccgattcc ccatttacat
    63301 cctgataata tgtttctagc agcatgaaat tgctgataaa caggataatc catgattttc
    63361 tcttgtctat acgattcttg ttcgatgatg tcccacggat gaatgacttg tttgacggca
    63421 tgttttgaga tttctgttga ccatctctga ttgcgggcag gtccacgtaa accaatcggt
    63481 agtatttctg cgtgatccgt cgacgcacga cgaactgttc cgaacatctt ttcagttaga
    63541 acgacccatt caggcaaggt gaacccttct atttcatgtt tatcaaaaag gacaatatca
    63601 tgtgcttgaa tcatttttta tgcatcccat tcttcgtcca ttttggcacg gactttattt
    63661 gtttcgacac gtccgaatgt tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg
    63721 tcatttgtat tgcggacatc ttgaatggct tcattgattg cttcgatgac tttgctgaca
    63781 ttttgttctg aagcagcttg gtcggtgata tcttctagga aatgatagat cgctcctagt
    63841 tttgtataat tattgatatc gtaagcgata gctggaactt tttcagcagc tgcttcgatt
    63901 tcagcaaccg aacgttttgt cacacgtgca gcactttctt tagacatcgc ttggacggta
    63961 atttctttat tgtttaaagc aatcaagcgg cttgattgta atccatgtgc taaaaatgca
    64021 cctgaaatag catttcctgc aatcaatcca ataactggat gtccagcttg acggagtttt
    64081 gcataagcag cagcactatt agccaaagcc acatggatac cgatcaattc ttcgttatag
    64141 ccgtatgctt ggcttggtac atcgatcaca acgatgattg gccgtttcac ttttttgtct
    64201 ttatcttctt cgtaaacgtg atccaaaatt tgtcccatgc ggaaaccttc aactaaacca
    64261 acttcaccgt ttcgtacacg gtacatcggg tttttaggat caggtacgat tgatgtcacg
    64321 atggcttctt ctatgaattt acttgattta ccataataca cagtactaat agaagaagtt
    64381 ggatttttca tgccagttag tgccgcaaac caatcgtaac caacacttgc tgcagctggt
    64441 tcatttcctt ctgctacttc tggtaagtca attgattttg cttgatggtt agcaattgct
    64501 tcattgtaat cgtcaatggc cattggtttt gaaagatcta aagaatcaag taatgataaa
    64561 taaaatgctg agctttcact gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca
    64621 gcagctttga ttgcttctac actatcagtc actaattcat caatgatttt tgattctacc
    64681 cgttgacgag atcctaatgt attccaaatc aagtctttgt cactagaatc aaattctcga
    64741 acgcctgctt cttgttcaat aacttctggt ccattcaaac cgactctcgc tttttttgtt
    64801 gcgattaaat aactcattaa tgctgaagta atcgacatac cgccaaatga tcctacacgt
    64861 cctggaatca aaccgatgac tggtacatat tgttttagag caatgattac atttccgatt
    64921 tctgaaatag ataacagtcc gtagtttgct tcttgcaaac gaacaccacc tgtatctagt
    64981 acaatgactg gaaagatctc atttcctttt ttattttctt ctaacacatg ttcaagtgca
    65041 gcaacgattt ttgcaccgga aacttcaccg ataccgccac cttggaattt tccttccata
    65101 gcgattacta ctgcttgttt gccattgatc gtgccacggc tgacaacgat cccgtcatca
    65161 ctttcaggga cgattccttg tgctggtaaa cttggcgcga tgatttgatc gaatggatca
    65221 atcaattcac gtccattgtc aagtagagcg ttcactcgtt ctctggcgtt taattctaca
    65281 aaactatttt tcatctgaaa gttcctccat tgcttgcgtc aatcgtaggt acacaactcc
    65341 tggtgtagca ccgaagtcat tgatcacgat atctgctgaa attggataac gatcaaaaaa
    65401 tctcgttaat acattgttcc atacttcgcc gaagccatcg cttcctgtac aaatttttac
    65461 agtagattct gttttttctg ttggaaacat caagatttcc aaatctcctg aaccgaccac
    65521 tcctacatgg attttcttat tgatcggctg tttcgttgga tatttataag ctaattgttc
    65581 catggatctc tccccttttt gtaaaacaga gcatacacgt tctgcttatt cgtctttctt
    65641 cttccatctg ggtaaatggc gacaaaaggt ttttctcgcc aattttatga ccaagttctg
    65701 aatttcattg gtggttcata caggccatct gaccattcga tcaaatcatc gatggtttgt
    65761 gcagctaata atgaacgttt cgcttcacta cgtttgatgc ctagatcttc tggtaatttc
    65821 acgatgccgg ctgcacgcaa gctatctagt gattctttcg tgctagtcat tccaagaggt
    65881 gtaacaccgc caattgctgc aattgctgct tgtcgttctt cttttgtttt tgctttgtac
    65941 aaataagcaa ttccttcttc tgtcacgata tgtgtagtat cttcgctata aatcatcact
    66001 ggtgcattgg ctaagcctgc ttgtttttga acagctagtg catctaattc ttcgacaaag
    66061 actggttttt tatttgaacc atatgtttca accatttgaa caactaattt ttggcctttc
    66121 cctaatggat catcattgtc gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg
    66181 ccaggattat gtcccatgtt aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg
    66241 gtagaagagt ttccttcgta atctaattgc aaggttgaac cgataaacat atcgatggca
    66301 tattgtcctg cagcttgtga taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca
    66361 aaaaagacat ctgggcgagc agcaatatat ttttccatac caacttcgcc accaaaacta
    66421 tggatgcttt caacccaacc tgattcgatg gctggaatca tcgttggatg tgggttcaat
    66481 acccagttct tcgcgatttt cccttttaac cctagtgatt caccataagt tggcagtaac
    66541 aattcgatag ctgctgtatt gaatccaata ccgtgattca aagattgtac gccgtgttct
    66601 gcgtagattc ctttgattgc catcattgcc attagaattt gcaattctgt gatgttttga
    66661 ggatctcgtg tgaataaagc ttctaattga taaggtttat ctgctttcac gacaacgtct
    66721 acccagtcac cagggatatc tacacgtggt aaagaatcga caacttcgtt gacttgaacg
    66781 atcacgatac catctttgaa agctgcagct tcgatgattg ttggtgtttc ttccgtattg
    66841 aaacctgtgt acaagttgcc gtctttatct gctttatctg cagctactaa aacaacattt
    66901 ggaatcaaat cgatgaataa gcgtccataa agttccaaat aagtatggat atcacctaat
    66961 ttcattgtgc cgtcttcaat catttgggcg acacgcaagc tttgtgcacc agcataagag
    67021 aaatcgatct tgcttgcgat tcctttttca aaaatattca aatgttctgg tcgtgagata
    67081 ctagacatga tcaaatgcag atcattcact ttttctgcat ctactttttc aagactttct
    67141 gaaagaaaac ttgcttgttt ttgattgtcg ccttctaata caactttgtc gccgggagca
    67201 atcagttttt ccattacttc tacgattttt tcagtttcta catacttgcc attgagcaag
    67261 ttgctgattt tttccaatcg ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt
    67321 ggtttttcca taattagtca tctccatttt tgatatagaa acgtggcgtc atcaatacca
    67381 tgttggtcta gattgatcgt acggatccct ttatctacac caaattttgt atgaatcagc
    67441 tttgtcaaag tattgccggg gggaaattca atcgtcagat ccattcctaa ttcttttgcc
    67501 aagtccatca tttggttcca ctgcacaggg aaagctaggt tttccaacaa atctttacga
    67561 atctcttctg cgtcctgagt ggctcttcca tccgtatttt ttatatacaa acatctagca
    67621 tcagatagat ggacagactg catatacggc ttgaatttct tgaccgtatc attcatcaag
    67681 agacagtgag atggcacagg gactttcagc agcttcgctg tttgcgcttg gtgccgtttg
    67741 gcttctgcca acgtaacgga taaaccttcg atactcccag ataacgtatg ttgcatcgga
    67801 caattttcat ttgacagata aactggatgg tcaggatcaa atgtttttgc tacaatttcc
    67861 tcagcttcgc ttctcgtcac acctactact acgcccattg cataaccgct tggataagct
    67921 tctttcatta agactgcgcg gtgatagact agttccgctg cattctcaaa tctcaaagat
    67981 cgacaagcaa cagctgcaca aaaagcacca atcgaatgtc ctgctgcaat atcaggctga
    68041 ataccgtttt tttccatttc atctaaataa aatactgctt ttgtcagtaa tgctagctgg
    68101 ataaaaaccg tatcttgata gttttcttct ttatcaacta accgataccc cgtcacattt
    68161 tctaaacgtt ccatgtacat ttcagggata tccgctaaca tccctaactt ctgactgcct
    68221 tgtccaggaa ataagaaacc tgtactcatg agcctcgcct cctcagcact tactttttcc
    68281 actgctatct tagtcaattt ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa
    68341 caaaaaggta tggatgggat ggataattat gaaattgctt catctgcagt attttaaaaa
    68401 agtattcgaa acaaaaaatg ttacccaagc agccaaagaa ttatttattt ctcaacctgc
    68461 cttgagccgt gcgatcaaac atttggaaaa cgaattaggt gtccctttgt tttaccatag
    68521 cggcagaaac attgagacga ccgtttatgc agaagaattt tatccatatg cagtcaagac
    68581 attagacaca ttagaagaag gaatcaaatt attgtcttcg gtcaatgaga agatcgttac
    68641 ttccgtcata ttgtatttag aggtagcctc tgtttctatt ccgaacttgg tccgaatttt
    68701 tatggaaaag catcctgata tccaattaac gatcatgcaa catcatttac cagatgatat
    68761 taccgatcct gtactgtaca tcacttcgga gatgaagcct ggcatgacca atattcctat
    68821 catgaaagaa cctgtttatg tcgccattcc taagaaacat cctcttgcta aaaaagaaca
    68881 tctgcaatta gaagatatcc aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt
    68941 ccgtcataca ctggatgcag cacttgaagc gaaaagtatc gaattgacga tcagttcgac
    69001 gacagatgat cctgcgacat tacgttctat tgtcaagcaa ggattagctg ttagcttttt
    69061 cccaaaagtt tcttggtctt acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga
    69121 tttgcccttg acacgaacaa tttatttatc ttctagcttc acagaagatc atcctttgat
    69181 caaaatgatt gcaaaaacga tgcgggaatt ttatttagga aaataaaaaa aaaagagctg
    69241 ggacaaaatt cttgtcgcag ctcttttttc gaacaaacgg tgttccagaa gtaatttctt
    69301 agaaaataag ccgaaatttc ccaaaaattt gagaagcaat tttcgtaaat tccttcttat
    69361 ttcttgaagc taaacatttc tgtcacaacc tcttctctat tgtcctagac attcattgcg
    69421 ttgatttaaa acttccatca atcaaatctg ctagttcatc tagcttttct attttacgta
    69481 gatcaataga gatccaatgt tctttattca tgtgataggc tggaaaatac ccctcttttc
    69541 ctcgcaaaat tccgccaagt tcaggatcga ccttcaaatc aagaatattg atgatcctat
    69601 ggtcacttgc atcatcatag attttttctt ctggaacaga catcaggacc ccaaaccatt
    69661 ttttgctttt ttgatgtctg aatacgcaat aatcaggaaa ttttttaaaa atatattctg
    69721 gagacaaatt gtatgtttca ttgatgtgat cgattagttc ttttttcgtt agcatctgct
    69781 tccatctctt tcttattgat tcgctttatt caaagatacc acgtaagaat agaaaaatga
    69841 atacaagtag ttgctaatca aatacttcga tatttttctt ttttatcaac tcttaaatgt
    69901 tcaatccgcc tgtgttggtc ttgctaccat ctactattaa aatgtttatt attgtttgcc
    69961 cacctatttt ttgattttag tggtatcatt tttcaagttc atgatgaaat tcttatcatc
    70021 agtaaatgct ccagaaaaat aaacaatgat tgaaaaatca gttgaaactt gaccaccgtt
    70081 tattttctcc gtattcttag aatagaaggg agtctgattg atgtattcac taatgaaaaa
    70141 aatcattacc gaaaaagata ttcgacgtca tgtttcttta gtagaacaat tgctgaatca
    70201 tacaaagata accgtaaatg aattagctga aatcataggg acaaccgaac gaacgatttt
    70261 ttctgacctt caatctattc gctctcagct gcccgaaggc tgggacatca tcagcgatca
    70321 agcaggaatc agtttgcaaa accagcaaaa tttattaaca aatgaccttt gggagatttt
    70381 tttcaaacaa tcagtcagtg tcgaactgct gaaaaatctg ctttttacaa aaaaagttgc
    70441 tgtccctgat tttctagccg accatgggct ttcttatggg acattgaaaa gacatgtgac
    70501 taaaatcaat cagcgtttag caagttacga cttgcagatc gatttgacga aatatacagc
    70561 ttgtattctt ggaaaagagc gtgcgattcg tactttttat catcgattgc tgattccttt
    70621 cactcataac aactattttt tcgaggatta ttcgatccat gaatcccatt attttcaatt
    70681 tttaagaaac ctcagtcaaa ctgaactagc ggttgaaaca gaagagattt tcggaacatg
    70741 ttggttcttt atcaatacga tccgtataaa agcgaattgc cgattagatt cttctatcca
    70801 taccaacagc acactctcct ccctgtatga ctctgcattg aaaaaattgt atttaacgga
    70861 aggtatttat cttaaagaca ctgagctttc ttttgcttct ttctgctttc tggaaagttg
    70921 gaattacaac aataactatg gacaagaaac cgctcgctgc ctgcatcatt ctccattttt
    70981 agaggtcctc gaaacgtttg tagaggaact agcttcagag ctgtctcttg atcaactaaa
    71041 aaaaacatct ctgactgata acttggctct cctcatatta aaatatcatg aatcgccgat
    71101 attgattgaa gcattggacc gtcaatacca tcgtttttta gaaacatatg atcagcaata
    71161 tgcacacttg tatcaacaga aagagaattt actgaacgat ctaaaaaaag aaatagaaat
    71221 caatgaacca aattactttc tccaactctt gtctctactg atccaacaaa cgattttttc
    71281 tatcaaaccg aatttgttca atgtctattt tattttccaa ggagaacctt catggaaggc
    71341 gttcttgcag caggaattaa aagattactt aggaaaacgt gtctgcttct tacctatcga
    71401 actaaccgat ctttccacta tttcttttga aaaaacagat atcttgattt ccaatttccc
    71461 tctagattct ctagatatcc ctattgtata catttcttct attccaacga aaaatgagct
    71521 caatcgtttg actgaactca ctttcaaaac ttttttatag taacggctag cgactgtatt
    71581 ctctagctgt tttttctttt ttgcgaaaca gtttgttcat gatccctttt tctatttcta
    71641 caactaagaa tagaagaatc acatttgctc caatcaataa ctgttgtgtc atcgttaaac
    71701 ttactgtacc gatcagctgc tggccgactg gtagaaaaat aaccatccct tgcaataaag
    71761 caaggatacc taatgaaaga aaaagcgctt tgttttgcaa aagaccttgg ttcaatgacg
    71821 gcttggacaa ctctctgcaa ttgatcatgt aaacagcttg agctaagacg atattttgca
    71881 gaagaatggt ttgctgtaag ctttgccctt caaactgcat agccaataaa taggcaggaa
    71941 ccatgatcag tagggagaca tagatgatcc gaaacaaatg ataaccagat aggattcctt
    72001 gctttggatc tcttggtggg cgcttcatga tttctggatc tggcgactcg aaccctagtg
    72061 cataagataa agtgattgtc gttaccatat tgacccataa aatctgtact ggggacaaag
    72121 gtagcgggcg attcagcata agtgcccaaa tcaagatcaa tccttgagct aatgcagttg
    72181 gcaagaagaa attgatggtt ttttgcaaat tatccaaaat ccgccggccc tcttttacgg
    72241 cttttgcaat cgtatggaaa ttatcatctc ctaaaaccat atcagctgcc tgtttgctta
    72301 cttcactacc tttgattccc atagcaattc ctacatcgga tcttttcaaa gcaggtgcat
    72361 catttacgcc gtctccggtc attccgacga tttctccatt tttctgcaaa gctttaacaa
    72421 ttcgcagttt atgttctggt gtcgttcgag caaagacatc gactattggc acttgctgaa
    72481 tcaactcttc atcagacata agatccaaat cgatcccttc taaaactttg gcagtatgtt
    72541 ttaagccaat ttgttccgca atcgcttttg ctgtgtcttt gtgatcccct gtaatcattt
    72601 tcactgagat ccctgcttgt tggcattcct ttacagcttt gactgcactt tcttttggcg
    72661 gatcgatcaa tccggcgatc cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt
    72721 cctgtttcga atctactttt ttataagcaa atcctaaaac acgctgacct tttctcgcaa
    72781 attctgcagc tgtctgactc catgcttctt gttctgcaga agaaagagtt gatttagcaa
    72841 ataatacttc tggtgcacct ttgacgtaaa tcaccgctcc tttttcctcc gcatgcatcg
    72901 ttgccatata tttgtattca gaactgaacg gaatctttgc ttctattttt ttcaaaggta
    72961 gattggctgt ttctgtgtgt cgaatcagcg caacttctgt cggattgcca cttaaatttt
    73021 ctgttttctg ttcttctttt gtttttaatt cctgacagtt tttcataatg gacaacacgc
    73081 aggcttcttc ttttgcagct acatccacaa ctgtcatttc attttttgtc aatgttcctg
    73141 tcttatctga acagatcact gtcatagaac ctaatgtttc taccgaagga agtcctttga
    73201 tgatcgcctt ttcttttgcc atttcatgta ctccaacgga tagaatcatg gtcaaaacag
    73261 ctggcaatcc ttcgggtacc attgacacca ccaaagcaat cgtagccgaa agaaggatat
    73321 gccaatccat accatatcga aatgtcgtaa aaataactaa gaataggctc aaacaaagaa
    73381 tgccttgaaa aatttgtttg tttaatcggt tgattttttt aatcagaggc gtcgtttgtt
    73441 gttcgaccga ttgcaatgct tgattgattt taccgatttc tgtcgtgttt cctgtttcta
    73501 caacgacacc gactgcagaa ccagattgta caagcgttcc tgaaaaagcc atatttttct
    73561 ggtcgccgat cgattcttct tctgtcaaaa cggcatgact tttttcagat gcttctgatt
    73621 ctcccgtcaa aatcgattct tccactaata aatcatgtgc ttcgattaat cggatatctg
    73681 ctggaacgac atctccggca cgtaagacaa tgacatctcc ttttaccagt tctttcgcag
    73741 ggattttttc ttgcatcccg tttgatagta ctatcgcttc ttgtcccatc agctgcttca
    73801 aaccattcaa tgattcttcc gcttttcttt cttgccagta actaacgagt ccattgacga
    73861 tcactacgaa taaaataata cttccttcaa tatattcact agaagcaaat ttcaaaaaag
    73921 aggcaatgat caacacgatc atcagcagat cagtaaaatg ctttgctatt tttttccatc
    73981 gttttgtctg ctgccttgct tttatttcat tatatccttg atcagtcagg cgtttctgtc
    74041 gttcttcttg cgacaagcct tctgttgatg tttgatagtt tttcaataca tcttctaccg
    74101 tcaatctgta ccagttcatc ctatcccctc ctgtttaatt gatactgtta ttatacgcag
    74161 ggaagttttt tcaggcgtat cgattatttc aggggggtct gaaataattc gttttgcaaa
    74221 atgtatctgg gcctaactga tatttctatt gcttattctt ttatatacaa aatttaagaa
    74281 tacaaaccag ataaccaata aaaaaggaat aaagcgtgtc gtgctttcga ataacaagct
    74341 gaaagcaatt gaagtaaaaa acaccaacaa agcaagatct ttatatcttt gtgtagtgtt
    74401 ccgtttcttt tctttaataa attgaacatg tgcacacaag atcgaagacc acacaaatag
    74461 aaaacaactc gttgtcatgc ctgccaaaag ctgaaaaaac aatcgactat cagctacaaa
    74521 aaaatgtaga caaagaccgg caaaaaataa cattcctgta attaaaatac cgtgtaaagg
    74581 tacgcctttc ttagaaagcg cacaaaacca atggggcaaa cttttttctt ttgctgtttg
    74641 atataaaatc ctgctagtac tgtagactgc actatttgct gaggacaaag aagcaaccaa
    74701 aatcattaga ttgacaattg ctgctccgcc ttggcaatgg atcacttgca gcatagtcac
    74761 taaaggactt tcttttaaat ctaaacaatg ccatggaaca acaaataaaa tcgacaaaat
    74821 cgttcctata tagaaaaaga gaatacgcca aggcagacag ttgattgctt ttttcaaatt
    74881 cttttgcgga tcacttgtct cgccaacggt taagccgatc atttcaattc cagcaaaact
    74941 gaataaaacc ataggaaatg agactaccat tccagcaaag ccaaacggta gtaattcatc
    75001 gatctgtcca aaattttctt tgattccgat agactgggta tgaaaatctg agacactcaa
    75061 tcctataccc aataagataa aaaggaaaat ccccaagatc ttgatgattg agaaaaaaga
    75121 ttccaattca ccaaaccact tgactgcaac aaggttgact gccattaaga tcattagtgc
    75181 gctacctccg cttatcaaag ctggtacatg tacaaaccag aaatggatat acaatcctat
    75241 tgctgttaat tccgcaagtc cagctccaag ccagcaaagc caataagtcc aattgacaaa
    75301 aaatgcccac tttttgccta aataatagtc aacgaaatca ctgatagagt taaatgtact
    75361 atttgctaaa aataaatcac ccatggcccg catcaaacca taaatagcca aaccgcatat
    75421 caaataaacg agaataacag ctggccctgc tgatgcaatt gcctttgctg aacctaaaaa
    75481 taatcctgtt ccaattgcgc cacctaatgc gattaactgg atatttcgat tagttagagt
    75541 ccgtttcatt gccggttttt ccatcaagtt cacctttttc tttttcaagc agcatggcga
    75601 cttcctgttc ccaagaaggt ttctttattt tttcaataca gagtggtact acaaacaaaa
    75661 tgacactgat ggctgtgact gcaattagat tgatccatgt tccagctttc atcgtcagtc
    75721 cgacagaaaa tccgattgcc gcaaataaaa caataacgac agtccaaact ccaagattgc
    75781 tctttttctc tttttttcca attctaaatg gtcgattcaa ctttgcatgt gtataacgca
    75841 atctcaaaac tgcgatcccc atgatgacat agacgataca gtagataaca gtagttgaat
    75901 ttacgagcat caaaaatgct tgattgacgc caggaatcag caaataaaaa agtgcaaaga
    75961 ttgagataat gacagactga gtcaaaataa cggatttgga tacgtcaaat tgattcgtct
    76021 tccaaaaatt gaattttggt ggatacgcac cttttctagc actttctgtc actgtctttg
    76081 ctggtcctga ggcccaagca gataattgga ctgctacacc aatgaagaca agtaaactaa
    76141 acagattcac aataagatgt ggcaaaccaa gaatttggca ataaatcgaa atagattgcg
    76201 cgatattatt caattccatt tgtccttttg gtacgacatt tgctacaact aaagcattca
    76261 aaatattgac gaaaaatata aaaattaacg cggcaaatac tccgcgaata tacgttttaa
    76321 ctggattttc caatcgagtg atatacacag aggacatttc gataccagta aaaataaaca
    76381 tgatcggcgc aaaataaaca aatgaggatg cagtcgtagt atctggtatt aatttgtccc
    76441 atgaaaaact gcctaatgtt ccggttggta cgatcccaac tttgatccac gcagcaaacc
    76501 ctaataagag catcatcgtc aaaggcacgt acaatcctaa ccaaacaccg acttttccgc
    76561 cgatttttgc catatcaaat ttcaaattca aaagagtgat gatccaataa acgactaaaa
    76621 tgacaactaa tgtaaatttg ctgttcaaac caagcggcac attatcgata atattaccaa
    76681 acaacggagc gagtgctgaa gcgaccatca ccatcccagg aaacatttgg acccataaca
    76741 gccatgagac aacgaacccc catttttttc ctagagcatt cgatacccaa agttctggac
    76801 cacctttttg gggaaacatt ccagcaaatt caccagagat caatgagatt ggaaaagcgt
    76861 acaataatac tgcaaccagc atgtaaaaaa tcatcgtcca gctcgttgct gctacatctg
    76921 gaatatttcg tacacttgct acaagggcgc aggtcattcc gataaaggtc atggtcgtga
    76981 tttgctttga tgaaccagta tctttcatct ttattccttc tttcaatagc gacatatgtt
    77041 tttgttttct tttatttttg ttcaagaaca gaaatgacaa ggtttcatcg attcagtaca
    77101 aaacccagta tcaccgaaac atccattgaa cgatcttagt gaactgggtt tgatacatga
    77161 aacgaacgat ccaacttata gaacgtttta taaaaaagaa tatcttggcg aggaatatat
    77221 gaaaaacagg gggaaataca attgttttga tgaagcaaat aattgtctgt tctccatatg
    77281 tctcttgtta ttctttcaca caaaaatcag ttctattgga tgatttcgtt tagtttcttt
    77341 tccattgctt tggcgatttt ttttgtataa aaattgaaaa ttcgatcatc attcaaataa
    77401 ggagtcatga tagccgcacg tagcagagtg attttctgtt cttttttcca ttcttctttc
    77461 gttagaccca ttctttcaac aaaacgaatc ggagaatcac cgtaatcttc ttgtgtaaat
    77521 gtagtatgag aagtaataaa ccgttcacca taaacatcgc catccatata agaagaccga
    77581 tcgaacattt tttcatttaa ttcattgata gcgttcaaat ctgtacagcc ttgttctttg
    77641 aatacccaat tgaccatatt aaaatcagga tgattcaatg gatagacttc gattgttttt
    77701 cctttaacag taaaagtcag atgatcgaga aattctctga atctttgagc tgcctcgatt
    77761 gaagccccaa tcaattgacc atatccggtc acattcaatg gcaatacacg atgtgctgtc
    77821 cagactgcgg cggcggttgc tccagctttt gatccttcca aaatataagc tcctaacata
    77881 tctggagcct ttactgattt ttcaaacaca tatggcgcga aataagaaat gatatttctc
    77941 atatttttat gtttgattac gattcctcca gcagcataag ggacatagcc catcttatga
    78001 ggatctatcg tgacagaatc cgcttctgaa atagctctga acccttcata tacctctttg
    78061 tcaatcgtta cgcagtggtg gaaaacatga tgttcttcaa agaattcagc taaggaagca
    78121 tacggcacaa attctcctgc ttcgtttaga aacagtgaac gcgcatagcc gccatatgcg
    78181 gcatcaacat gtaaatagaa atagatccct tcatctttta acctttctct taattggacg
    78241 attttatcta cactgtctac ttgaccttct tctgtcgttc ctacgacagc aaccacacct
    78301 agaattggga ttttttgtcc agctaattca cgaattgttt tttccaaagc attaatatcc
    78361 attcgataat cctcttgaac tgggatagca accatctgat ctaagccgac tccgcaaata
    78421 tctaacgcct tcatccaaga atagtgtttt gtttgaggga ccagccattt ccctaatctt
    78481 tggatatttt ttccgctacg ggaagaagct gctttgacct catccatttc ttcgtccgta
    78541 aagctttcgg tcatttccaa aatttcttca acagataaat tcaaaagttc ccactcagac
    78601 atttttttca ctttttcagg cagcacttcc ttgacagcta aaggaatcga tttgatgcaa
    78661 cgggcgtacc ataatccttc aagattagca atagaaccat ctgctgcaat gtgtccccat
    78721 ccgtctgtca tattgaataa tgaagcaaaa tcctgtccta cttctgcttc catttgcgaa
    78781 gttgccatag aagattccaa tgcaacatta tttgggttcc acagcatcgc atagttgtaa
    78841 gctaataaag caggcatcaa tgtttcagca ttcatttgtc cccagtaacg gcctgctgaa
    78901 tgccatggga tcgatcctgc gcgtaatcgc tgactgactt catccaagac ctctatcata
    78961 tggtcgcggg tggctagata atctggagtg agcttatctc cttcgctaat tgcaggcata
    79021 tctgaaggaa tataattttc tctccatcca agatgttcat ctaccatctt gttcaacaac
    79081 attttataaa ctggtccatt ttcagctttg tcgcctataa agacggcctt gatatccata
    79141 tctttcattt ttcttccctc catattttgt tggtcaatgt cttgcagata catgtgttta
    79201 tctgtaacac ctcttcacta tagcgccaaa aatgagaaat ttttttttat aacatcaaat
    79261 ccacgtagat ttttacaaac aaaaataaat aaagtcggat ttttctgata attcaacgca
    79321 aaaaagcgaa caatttttca ataaatcagt cttctaaaat agataaccta aaaaaagcga
    79381 acaaacaaat aaaatgactg tccttcattc actaaacata tttttatttt tttatattgt
    79441 taaaaattca caatttattt tttaatagaa ttatctgttt tccaaatttt ataacataaa
    79501 aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat ttcaaaaaga
    79561 aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg ctctactatg
    79621 attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg gcaattctat
    79681 gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg caaaaccact
    79741 ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc ttatacacat
    79801 cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg caagatcatg
    79861 cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt ggaagaagat
    79921 gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa agcttattca
    79981 ttaaaaaaac aagtattagc ttactttaat gactgcggga tcgaaatcga ccgctactcc
    80041 cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc
    80101 ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg tttcattgaa
    80161 tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat cttatcgatt
    80221 ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt
    80281 atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg ggaaaacact
    80341 gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact attcaatctt
    80401 tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca gctacaccaa
    80461 gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga atctcacttc
    80521 aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg
    80581 gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa tgaggaacag
    80641 acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca gttaccttat
    80701 gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag cggaattctc
    80761 cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt tcattatctg
    80821 atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc accaacaatt
    80881 tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt
    80941 gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc tatttcgatc
    81001 aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc tgtgacaaaa
    81061 atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc tcttgataca
    81121 cgtcaggctc acaacctcac ttattttgga catctagggg tccgatctct ttatttaaaa
    81181 aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata tcctgcaagg
    81241 taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa cttagaagta
    81301 tatagcctcc caacatcttt ttaatagcat tgttctcgct tactcggtcg ctttgcactt
    81361 ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca ccccaaacca
    81421 ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa
    81481 actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt tgtcttctat
    81541 ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa
    81601 aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga ctagtacttc
    81661 caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac gagtattacg
    81721 ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat
    81781 cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa
    81841 ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt gttcctattc
    81901 ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta atgataacgg
    81961 ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga
    82021 agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag
    82081 aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc
    82141 tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct
    82201 tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa
    82261 ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc
    82321 aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca
    82381 ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt
    82441 tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta
    82501 aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag
    82561 aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg
    82621 atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat ccattagaaa
    82681 accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt
    82741 tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag
    82801 atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg
    82861 gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga
    82921 ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag
    82981 aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa
    83041 catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga
    83101 tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat
    83161 acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt
    83221 tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg
    83281 tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg
    83341 aagatacagc agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt
    83401 acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag
    83461 ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa
    83521 gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc
    83581 gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca
    83641 cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta
    83701 cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg
    83761 gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga
    83821 ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa
    83881 ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca
    83941 ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct
    84001 ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac
    84061 gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa
    84121 caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat
    84181 aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac
    84241 agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc
    84301 gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca gccaagctaa
    84361 aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa gcttttgaac
    84421 accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt
    84481 gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac tgatcccttc
    84541 tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt ttttcaacgt
    84601 ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg attaccatta
    84661 cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat agaatgtttc
    84721 atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa atgaaattat
    84781 ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc caatgaaagc
    84841 agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc caaaaatatt
    84901 agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct ccgatctgca
    84961 tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac acgaaccaat
    85021 gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt
    85081 tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca tggaaagcca
    85141 atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg gcaagttgaa
    85201 cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag actctacttc
    85261 atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat cagatatttt
    85321 gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat
    85381 tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg ctggagcaga
    85441 gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta attacaatgg
    85501 ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt atgaactggc
    85561 taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag agcctgtcaa
    85621 agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat tacagatggc
    85681 atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata tgactccagc
    85741 ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc atggacaggc
    85801 accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac tattcgatcc
    85861 tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat atgagatctt
    85921 tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc agtaaaaaat
    85981 acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg aaataggaaa
    86041 agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa atcgttacac
    86101 attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg gaagccttgc
    86161 ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg gtgctatttt
    86221 cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa
    86281 agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc
    86341 ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact ttcttccttg
    86401 accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag
    86461 taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa tatccgcttg
    86521 gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga
    86581 tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt
    86641 tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata
    86701 ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt cgcgctttgt
    86761 tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc
    86821 tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac
    86881 aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta
    86941 atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta
    87001 gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt
    87061 ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg
    87121 ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca
    87181 aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt
    87241 cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt
    87301 ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta
    87361 agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgtccagctg
    87421 cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga
    87481 ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta
    87541 aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag
    87601 aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag
    87661 agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac
    87721 catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg
    87781 aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc
    87841 ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc
    87901 cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac
    87961 cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca
    88021 attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca
    88081 ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa
    88141 tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc
    88201 ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatt aatgatctct
    88261 tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa
    88321 agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga
    88381 tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca
    88441 ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg
    88501 atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa
    88561 tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac cattcatcat
    88621 gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc
    88681 aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga
    88741 ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt
    88801 tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca
    88861 tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt
    88921 atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt
    88981 aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt
    89041 aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc
    89101 acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca
    89161 agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc
    89221 tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa
    89281 cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa
    89341 aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag
    89401 tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc
    89461 gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc
    89521 ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat
    89581 gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact
    89641 gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat
    89701 tttcttttag atacatggct atatcataca tgattttcgg atagatttcc catcccctgg
    89761 aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag
    89821 gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga
    89881 ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga
    89941 aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca
    90001 tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt
    90061 ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac
    90121 cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat
    90181 tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc
    90241 aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt
    90301 aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc
    90361 agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg
    90421 gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt
    90481 catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat
    90541 aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat
    90601 tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc
    90661 tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag
    90721 aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt
    90781 ttttagatcc catcattttc cacacgattt gctactttta caaatggcag atagatcaaa
    90841 atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc
    90901 agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca
    90961 aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac
    91021 ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg
    91081 ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca
    91141 actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac
    91201 atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc
    91261 gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt
    91321 ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt
    91381 aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg
    91441 tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga
    91501 atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt
    91561 ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag
    91621 accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct
    91681 actataacag tctgattttc attcactaat tcataaacag ttggattaac agataaataa
    91741 gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg
    91801 atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca
    91861 gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt
    91921 tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga
    91981 atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt
    92041 gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt
    92101 cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat
    92161 gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta
    92221 tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct attattttct
    92281 gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat
    92341 tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat
    92401 gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt gggataatat
    92461 gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt
    92521 gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca
    92581 tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca
    92641 ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa
    92701 aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc
    92761 gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc
    92821 ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact
    92881 gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt
    92941 cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt
    93001 ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa
    93061 cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc cttcgatcat
    93121 ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca ttcgattctt
    93181 gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat cgtttctatt
    93241 ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta tccatcattc
    93301 gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt gtaacagatt
    93361 tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt gatacggcga
    93421 ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct gaaaggaatg
    93481 gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa cgatttgtca
    93541 gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct tctaatactg
    93601 gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc tgatgaataa
    93661 tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct tctccattat
    93721 agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc aaagcttctg
    93781 ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg tcaacataat
    93841 ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta cgagagcccc
    93901 attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata atcagttcat
    93961 cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct gcagctccag
    94021 cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg aatgcgcctc
    94081 ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc cataatccga
    94141 gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc attttttcat
    94201 aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt ttaaaacgct
    94261 ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc aaaatgattt
    94321 tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa ttgattacgt
    94381 gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg atgatagttt
    94441 tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg gtggaaagtt
    94501 cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg ttcgacttga
    94561 taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg
    94621 ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc tcttgtgtca
    94681 taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg atcataattt
    94741 ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc ttttttcgat
    94801 tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg ttcttgatag
    94861 tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc aaatccttcc
    94921 gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat agctaatgga
    94981 cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc tcttatgtca
    95041 ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt caaaatcaga
    95101 ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc tttccgaaga
    95161 agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag cgattacttc
    95221 atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga ttctcctttt
    95281 taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac tattttaaaa
    95341 aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt tactgtataa
    95401 cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac caatccatta
    95461 gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc taaataagca
    95521 tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat gatagaacta
    95581 ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc gtgttttatt
    95641 atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa ataatccatt
    95701 cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc cggctgagaa
    95761 attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa taacaaaaca
    95821 gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt gaaaaatatc
    95881 atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca aggaaattgt
    95941 aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt tgtagctagc
    96001 caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt tttaaataaa
    96061 atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt agttaagtcg
    96121 ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata taaatttgtc
    96181 acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata ccaaaaagca
    96241 ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat agcaataaaa
    96301 tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat aattaatgct
    96361 gtactcatta atgttgatag tacatggctg tacactagca aactcatact caatgctagc
    96421 tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata aaataataca
    96481 gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta gatgtctact
    96541 gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt tttttgtttt
    96601 aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa taaccaaacg
    96661 taaataatat agcttacaat caaatttttt gtcatccaat ataacattac agctggaaaa
    96721 aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa tgtctcataa
    96781 ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg aaaagaaaga
    96841 tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata tatactacta
    96901 actataatta atccactcaa tattactact tttttattta ttttcattct tcttgtttat
    96961 ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc gtttaaaact
    97021 tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga gagcagttgg
    97081 agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca cattcactaa
    97141 gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct gtgtacctaa
    97201 ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg tgtaagtaat
    97261 gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt ttacttcatg
    97321 tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt ataatcctcc
    97381 catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat gaccgtgttt
    97441 tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga gtagaaacac
    97501 cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt tcataaaaac
    97561 aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc aaggaaactg
    97621 gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag ttacgcatac
    97681 ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt ctttcagtat
    97741 gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg ttgtaatatc
    97801 gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct gcaaatctgt
    97861 tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat accaaaaata
    97921 gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca caaattttat
    97981 atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag caatcaatgc
    98041 actacacaat agtgtagtta agacatgagt atacacaagt agacttatac tgacagataa
    98101 agcgatgact tttttttctc tattgaatat caatccataa gcataatata aaacagctgg
    98161 gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat caactgttcg
    98221 ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct ttttaaaaaa
    98281 cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc aaatatatag
    98341 aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag gaattattgt
    98401 aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg tatagttgat
    98461 tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag ataaatcacc
    98521 tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag tactaagtaa
    98581 gataagaact actaaaagaa atgctttttt acttattttc atagatttgt tgttctcctt
    98641 ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt gcgttaaatt
    98701 tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt ttaaatagga
    98761 gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt tatttctaag
    98821 ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg tcaatcagga
    98881 tacttacttt catttatctc gaatcatagg actagataat gtatggagtt ctccagtaaa
    98941 cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat ggcttactct
    99001 ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt ataatatcta
    99061 ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga aacaaataaa
    99121 aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg cctatcgtgc
    99181 cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat ttttgccttt
    99241 gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt actggttgtc
    99301 tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg tttctgtatt
    99361 tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg ctagactctt
    99421 aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct taattccttt
    99481 tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat taagtggtat
    99541 ggctcctagt gatatgctga cacatatttt aaataataac tataacaact atactattgg
    99601 attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat taactgctga
    99661 tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca atttatttcc
    99721 ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc ggttaaatgg
    99781 tttttcttct ttatttatag cctatacgat gagtatagtg atctattata ttttttctac
    99841 taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat tgcatctctc
    99901 aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag cacctgaaga
    99961 cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag agtcaatcta
   100021 tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa taaatccaac
   100081 aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata ataaaaatac
   100141 tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta atggcaaact
   100201 agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc caggtataaa
   100261 taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat atttatctat
   100321 cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag
   100381 aagagaagta caacattctc actagatttt tatttaagaa agacttttta gctgtacaca
   100441 taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca ttatttaaca
   100501 tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt tatcgataat
   100561 aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt
   100621 tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg atttctagta
   100681 atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca aaagtaaaat
   100741 gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact gtggattttt
   100801 ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat ttttcgcaag
   100861 ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg tttcgctcaa
   100921 atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc accgcattat
   100981 atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata tcccgcggaa
   101041 ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt gttttaaaat
   101101 ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca ataatttcta
   101161 tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg gttttatcgt
   101221 catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt gtcaacttga
   101281 tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct acctttttaa
   101341 gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac aagaaaactg
   101401 tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt
   101461 tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacct ggcaatgggc
   101521 acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag taatggccaa
   101581 gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa actgatcaca
   101641 gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata gatcaagaaa
   101701 agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat attatggacc
   101761 catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc tgtaaagata
   101821 caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa cacacgagat
   101881 gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat ccctgaaacc
   101941 acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa tacactgatc
   102001 acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg agtcaaaaaa
   102061 tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata
   102121 cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt tatcttgatt
   102181 taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc gaatcatttt
   102241 aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa tctttcaatt
   102301 tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta aacaacagat
   102361 cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata ataaatccaa
   102421 taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat gctaatggaa
   102481 tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa gcagacaaaa
   102541 tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt acagcagtca
   102601 acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca cctaacggga
   102661 tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc atgtttatcg
   102721 ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa
   102781 agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa
   102841 ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga tactctgcca
   102901 atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc cctatcggag
   102961 caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga tccaccaact
   103021 cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc cagaataaga
   103081 cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca
   103141 tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt tcttgtaaag
   103201 taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa agcaatgttc
   103261 ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt ttatgcttgg
   103321 caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc aagcaactga
   103381 caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata ccaaacaata
   103441 ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa aacttctcta
   103501 acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc aaagaaacta
   103561 aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct aattttgtac
   103621 tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat taatgggcaa
   103681 cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg tttattcttg
   103741 aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg tataatgatg
   103801 gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac aaaccctggt
   103861 cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa tttgatccca
   103921 ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt
   103981 cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt ttacgtacaa
   104041 gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag tcagaattac
   104101 tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac agttggattt
   104161 ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac aattggatta
   104221 gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg acagagtatt
   104281 ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa ctggaaagaa
   104341 aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt cggtttgatt
   104401 attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc gctgaggttt
   104461 tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt atttttagaa
   104521 atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt atttttgatt
   104581 gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac atttatgaaa
   104641 cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt ttttatttga
   104701 tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa aatccgtttc
   104761 attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac tcagcgttgg
   104821 aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc agcatttttt
   104881 tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg gaaagaaact
   104941 atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct gacaatcatc
   105001 attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg gtacgccttg
   105061 actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa aatggaaatg
   105121 cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc agaactatta
   105181 tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct attattgatt
   105241 actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat tcattggaaa
   105301 cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt tttcattaat
   105361 cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa gactttcttt
   105421 aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa caatatcgac
   105481 attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg aataatgaaa
   105541 aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt aaacaatcag
   105601 acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc taatctgaca
   105661 gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac aggaggattg
   105721 atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg actgacaggc
   105781 ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct agtggagcgt
   105841 cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat ccatcctttt
   105901 acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga caggttctat
   105961 tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag cccacgcata
   106021 tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac ggaaacgtca
   106081 caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta ctatagtgaa
   106141 ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga attacaaact
   106201 tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca agaaatcgac
   106261 aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc aatctatagt
   106321 gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt catttataac
   106381 aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc tcctaataac
   106441 tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg
   106501 acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag cttgtctaat
   106561 cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca agaaaaaaat
   106621 ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata tgatcttgtt
   106681 gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta acaaataaac
   106741 tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca ttaatttgct
   106801 tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttgaag
   106861 taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt tggctgattt
   106921 tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa
   106981 tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa
   107041 acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg agggacttta
   107101 agaccatata agtccacact catttgattc gtataacttt tgtattgttg tcccattgtt
   107161 tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt
   107221 tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga agaacttgaa
   107281 gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga ggtatctgct
   107341 gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt agttgcaact
   107401 gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt ggtacaacca
   107461 gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta aaaaactcct
   107521 ttcgattact cccacttctt tattctatca aataagcaat gtgattttat tgatttttcg
   107581 tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct gatgttgtct
   107641 gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa tttacctcac
   107701 gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt cctcatccaa
   107761 atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt ctttattgaa
   107821 acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt cagctaattt
   107881 gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt tttccaacat
   107941 tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat aacgcatgac
   108001 ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag agattttatt
   108061 gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga
   108121 tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt taaaatttgc
   108181 taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat ctgcttgttt
   108241 tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct tttacttttg
   108301 ctgattggac ttcataacct aatttttcca caactgcagc taattgttca gggtggactt
   108361 catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc gtattgctta
   108421 ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga caagctagtg
   108481 tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc ttgatagtct
   108541 tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac gatttttctt
   108601 ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg atcaagatac
   108661 tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat aagccgttga
   108721 gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt aaaacagttt
   108781 tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc aagacaacgt
   108841 cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata tcagccgcag
   108901 ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat ccttgttctt
   108961 ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg acttcatcaa
   109021 tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc agcatcacta
   109081 actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct ggtcgaatca
   109141 catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata atgaccgagc
   109201 tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt
   109261 gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc tcagctgtga
   109321 ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt
   109381 tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac gtttctaatc
   109441 gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata gtggatacag
   109501 cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg attttggaaa
   109561 aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca ttaccgattc
   109621 ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa
   109681 cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca ataagtaaaa
   109741 cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg gatttggtat
   109801 cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct ccgaccttag
   109861 ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact tcatctccac
   109921 tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca taccctttcc
   109981 cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag acgatatcgc
   110041 cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg acaacactag
   110101 cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg gtcaatgttt
   110161 tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac tccttgtatt
   110221 ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt gagataactt
   110281 tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata atagaagcta
   110341 tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata aaaccaaaaa
   110401 taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc cacttttgta
   110461 atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag atacttatcc
   110521 atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt ttggtagaat
   110581 acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca actattgtcg
   110641 tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga gatctatttc
   110701 tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc cacgtcgttc
   110761 atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc ctttctctct
   110821 tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat caggatttgc
   110881 agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg caagcatttg
   110941 ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt gtaaaaaatc
   111001 gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt ctccttttat
   111061 ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa tttgagttgc
   111121 tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa gttcattttc
   111181 catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt tgtaatcttt
   111241 cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc aatgattgtg
   111301 ttatacttac attataatag acacagtgtc tactatctat aatcaatcat ctataagttt
   111361 gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta aatgaaaaag
   111421 acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc cacattatta
   111481 gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc agatgtacat
   111541 cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca attagaaagc
   111601 aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag ctatgaggaa
   111661 ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg cttatttatc
   111721 ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga agaaaactat
   111781 ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat gagattcttt
   111841 actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga aagcaattac
   111901 aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca catagagaaa
   111961 atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac cttctcgtat
   112021 aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta aaatttctat
   112081 aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa gtgatatatc
   112141 ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct aatttattac
   112201 aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat catccagttt
   112261 attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca tcatctaaca
   112321 ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct tctggatgtt
   112381 tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta ggaattggat
   112441 cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa acaaaccact
   112501 cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga cgtttaatga
   112561 caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa attcaaagat
   112621 aattttctca ataaagattt ttgattccag taagattcct ttactccttt tacaacatag
   112681 cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat agcccaattc
   112741 tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg tttattactt
   112801 actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat cattattttt
   112861 atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact acataaacta
   112921 attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat gattgtttct
   112981 ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt gtccatttac
   113041 ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc ctttagattc
   113101 atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat gccattcctt
   113161 gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt ccgtgggtgc
   113221 atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt cttgattcat
   113281 taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg ttgcagcaga
   113341 tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt gcttagctga
   113401 tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt gaaagcctaa
   113461 gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag accatggaaa
   113521 aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt tttctttgcg
   113581 attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa tgattcctac
   113641 cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga catttaatgc
   113701 ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga tttgtcggaa
   113761 catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct ttgacaactc
   113821 cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc cgcctaagac
   113881 tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat aaatgagtaa
   113941 gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca gtgcgtaagc
   114001 ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag cagaacgatg
   114061 ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata taaaagccgc
   114121 atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag tacaaacatt
   114181 caatagccaa acatataaga tataactaac gattaaatta ttacttatcc aataaaaaat
   114241 gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc cataatgatt
   114301 aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga ctctagcaaa
   114361 atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat aattgattga
   114421 ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct ttacttgcat
   114481 tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa attttaaata
   114541 aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta
   114601 atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct
   114661 taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca
   114721 tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt
   114781 actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat gagtgaatga
   114841 tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga tggtcattca
   114901 tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac tagaacaaat
   114961 caattaaatt ccataaaata taaataatag acctagcatc atctggtata aaatggatgc
   115021 taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat catttctatt
   115081 ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag tttagataaa
   115141 tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa taacttccaa
   115201 tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc aactaaccta
   115261 attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac caagaaactt
   115321 tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa tcttaaatca
   115381 actagtttag aattactaat atcacagtaa ggcacactgg accaatttac acgtccctct
   115441 tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga atctgtatag
   115501 ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac atcgctagat
   115561 gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc gactattttt
   115621 tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct aagtctagta
   115681 ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg ccactttcaa
   115741 acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt ggaaatttga
   115801 ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc gaggtataag
   115861 gaccataaga acgactggct aagagataat ggccataaaa agccacacct tgaatcttat
   115921 cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg cgaattgctg
   115981 ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca ccttttagtg
   116041 tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct tgtggaatct
   116101 ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca gatgtatctg
   116161 ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct gtgattgtca
   116221 accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc cgtttaggta
   116281 aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata atagaatgat
   116341 gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa gccaaccctt
   116401 gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc attcttgttt
   116461 ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct gcttgaacaa
   116521 taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc ttccaatgct
   116581 taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa atcattattg
   116641 ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc catataccgc
   116701 tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga tctttcataa
   116761 tctcatttat agtaacatta agaatccctt attctactat agtcacttcc acatcaatat
   116821 tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg attttctctg
   116881 cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt tggtagcccc
   116941 catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac tcaagattat
   117001 tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca gccgcaaaaa
   117061 gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc atgtgcatac
   117121 ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt gctgtttcgt
   117181 acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg taacgagtat
   117241 tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca acatcgatga
   117301 tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata tttggtgtcc
   117361 ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc ttcacactgc
   117421 tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata gttaaagaaa
   117481 aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact aatcgacgga
   117541 atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa aacaagataa
   117601 aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa caatgggaaa
   117661 agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt cgatagatta
   117721 caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc acctatccca
   117781 tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc aacaaatagg
   117841 tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga ccagaccaag
   117901 aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt agtcatttga
   117961 ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact catcacacct
   118021 aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc ctacttttta
   118081 gtgattattc gatacatcca acaatcaaat tttttctcat tttaacacat atttttattc
   118141 tagttgtctt ttgaacaatc aataacataa aggtgagttt cttctacatt ttaaaaaatc
   118201 gaatggcact gaagagttca gttgtcagca tcaatctcct actcaaaaag gcaaaaaaac
   118261 gtaagtcaga tgttgttgac atctcactta cgcatccatt gattgaaaat ttcttagtaa
   118321 aatctactag cctaaaaatt tgaagacttc ttctcgatca tacagtttaa atactttctc
   118381 gcataaaaat tgagtaatta gtgccgcaaa aacagggatg actaaccaac aaagaacaac
   118441 aagaaagcca tttagccctg catctaaaga agccaacgga ccaacaagtc cgaccacgcc
   118501 gaaaccagca gaagcaggcg tacctgaaat agaaaataga gcgacaggaa tagctgtaat
   118561 cactgcacta actaaacaag gaataagaat cacaggtttt ctaaatagat taggcatcat
   118621 catcttcata gcgcctaaag caatcgcaat tgtcacacct gacttattaa ccttccaaga
   118681 gttcactact aaaacaaaag tggttgcagc tacgcccatc gcagccgcac cagcagaaac
   118741 accatctagt tggattgcca ttccgattgc tactgttgaa ataggagaga taatcaaaaa
   118801 ggcaaaggaa caaccaatca agatagacat taacagcggt tgtaaggtag tgaagttatt
   118861 aatcaatgat ccaattgcag aagtaattgc tgttacaaaa gggaataaat aaaaaccaag
   118921 taaacctgca cctacaccta caacaatcgg tgttaacaca atcgacactg acccaaaact
   118981 atttccaatc agtaaaatca gcccgacagc aatcgatgct gtcagcatcg tattaataat
   119041 atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc aaatcaggtg tgaatttgac
   119101 tacaccagaa ccaacaaaag aagctccggc tacaaccatc attctcatcg gatcaaatcc
   119161 aaattgtaag gcaatcaaac caccaatgat caaaggtgtt gctagttgaa aaataactgc
   119221 taactgtgcg atcgttaccg caaagggata cgcactaaaa tattttaaaa tggcacctaa
   119281 tactgcatta ggaatcaatc caataatgat tcctgttgct gtaccagcga ggactttatt
   119341 cagaaaaatt ttcggtgata ttttttgtga agtttccatt caatctctcc tttattttac
   119401 gttcaaacta tcttcttttg catggacaag ctcagtcaag aaatcacaat aaggcgtcgc
   119461 aacaccatat ttcttccctt ttctagaaat tgcgccattg atataatcga tttctgttaa
   119521 tcgatggttc ttaatcaagt cttgatacat tgaaggataa tgtaacccaa ttgtttccgg
   119581 gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca tctagttcaa tcttctctac
   119641 tgctgctact ttggcaaact cattgacaat cgtcgccacc attttatgag cagtcgatgt
   119701 ttttcctagc tcagccatat taacgtctaa gattgtgcat agtccgttca ttgttccgtt
   119761 cacacatgct ttgcgataaa tagaatagtg aatattgtca gaaaaatgag catttaatcc
   119821 tgattcagac aatttgtctg ctaatttttt tgcagctgct tcttttccat cacctaaatt
   119881 ttgtaattct actgaaccac ttccaaataa tttgacctga ccaggacctt caagacctgc
   119941 cgtccacatg gtatttccaa tatagatatt ttccattggt acaaatttct caataatatc
   120001 ttcatgcccg ataccattta atagacataa gacctctgta tcttttttga ttaatgattg
   120061 gatatcctgc agcatttttt ccagctgcat cgctttggta aatagaataa tcagatcaac
   120121 ttgatcttct ttttctactt cggattgaag aacgattggt agttttgctt ctacctcttt
   120181 accattaaaa tttgcttgta atccatgttc tttgatttgt tggacatgtt ctgcccagcc
   120241 atcgattaat aatacttcgt tcccactttg gtgaagcatc aacccaaagc gacttcccat
   120301 tgcgccagcg cctgcaattg ctattttcat tgattcttcc tcctttttct ttctattata
   120361 ctcatttgag aatcttttca caacctcgtt ttgttattct tctcttattt atcatgttta
   120421 tatccaccat tcaagttatt ttatcagcaa atcaataaaa actggaatag aatcccaatt
   120481 tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag aaaaaatctc
   120541 actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc ttccattcat
   120601 tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt tgcgcaacac
   120661 agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt taaaaacttc
   120721 tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa aaatagcctt
   120781 ttattgtcta tgggaataat aatttaaaat tggtactttt ttattaatta gttcatcgcc
   120841 caataatctc tccagtttct tagcatctcc tgactcaacc ggaattatcc aagcccagtc
   120901 taaataattt tttaaaatct catcatattt tgcaaatatt tcattgccat attccgtcct
   120961 taaaattata ttttcagtgc cagacaagtg atttatattg agttgataaa aaagatctcc
   121021 atctaaaaaa atatcattag aagaagattt ccccacagta atgaccattc catcagctct
   121081 atgtgttaca tacaaataat tgtatgacct catttcacct aatgaatcaa ttatttcttg
   121141 ccctttagct ctaatatatt gatctctatt acctatctca ctcttattta acaaaagttt
   121201 tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac tactaatcca
   121261 atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg ctaaatactt
   121321 tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac atcgattctc
   121381 tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca taaaaccaat
   121441 ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct catcaaaaca
   121501 accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat aaattcctaa
   121561 ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc gttggccttc
   121621 ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct cagcaaaaac
   121681 gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta gcaacacatt
   121741 gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct tatctttttg
   121801 cggaccacgc gccaagagaa tcaggccatt taggaatgcg tcaaaaactt ggttgttgat
   121861 ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta ctttggtcag
   121921 taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag ctttaatcaa
   121981 atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat tgttattcat
   122041 ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt gctaagattt
   122101 ttaggatcaa tgataaaaac cgcatcactt atgataaggc gctgggtatc atgagtgaat
   122161 gatattgctg aatacaaata gtccgaccac attaagttga tcggacttca taagcatttt
   122221 attgactaat acttttttgc ttaagaattg attctaacat cttttgctaa agttaagcat
   122281 cctatagttc ctgcattatc tgctaactta ggtgtgacaa tgtattcttc gatatttggc
   122341 gtatcgacat agccgttaat tagttctaaa aaacgtcttt gtactttctt tttcagatgt
   122401 tcttgttgca tgacaccacc accaaaaatt ataatatctg gtgaaagcat cagggtcgta
   122461 ttacaggcac attgtgcaat ataatccgcc tcgatttccc agaacggatc ttctatagaa
   122521 agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc caacccttcg
   122581 agacaatcat gatgaaaagg acaagtacca gagaaagtat ctcctgaatg tctcttaact
   122641 aacgtatgcc ccatttctgg atggctaaac ccttcaacaa aaatcccatc ttgtatagct
   122701 cctccaccaa ttccagttcc aacagtgtaa taaacaacac tggataaacc ttttccatga
   122761 cctgaaacat actccccgta acaagcagca ttcacatcgg ttgtccaggc aattggaatt
   122821 tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt ttttggagta
   122881 gaggtgatat gtccataggt ctttgattca cgatggatat caatcggtcc aaatgaccct
   122941 actccaatgc tttctaattg ttctttatag tgattaaaga aatcaattac caaactcatt
   123001 gtttcattcg gtgtagttgt aggaaaactc actctttcta aaacctttaa atcatcattg
   123061 ccaatggcac aaacaaattt agtcccacct gcttctattg agccatacat ttcttactcc
   123121 accgtccttt tatctaggaa ttcgttcttc cctgtctaat agtttttcga aaaactcatg
   123181 tggttccttt tggtaccaat aggcaacaga agaaatatcg tcttgacgct cataaagctt
   123241 gatgtcatca tttcctaggg tttggaaagt aacacgtaaa ttttctttga aactaatggg
   123301 gtcgggtaaa tgccagcggt acaacccatg catcggtaaa gcatcatcgc caaaaccatg
   123361 aactggattt ggattaggtt ttccagcact aaaataatcc cgggtgtgat ctcttttaga
   123421 ttgaaaagga tagcccataa atagcgtatt aaaattttga gcatatgccc tttctccaaa
   123481 gttcttttta tggaaagccc aagcgccgcc aaaataatct tctgctcctg tagatgttat
   123541 tgtagggaaa tcttcatctc catctatata aaacttaaat tctccttccc cccaccagta
   123601 tctttccaat gcgcacaaag caaagtaggt tcctacataa tacccagtgc ctttgattcc
   123661 atcaataagg gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc atttagcatg
   123721 aaaatacatc gcatcatccg gtacttcagt caatgcataa ttaaatgtat aaaaaatgtg
   123781 cttaataaac tcaggatgtt cattcgttaa agtaatctta gctttttttc gaaatggcat
   123841 ctcaaaatag caattcatcc ctgatgttgg attcactacg attggtaagg aattcacttc
   123901 gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat ggactttcta cagaaggagt
   123961 ggattcatca tcccaataaa tacgcaaaat taaatcacgt aagacgaaac tcccagcgat
   124021 tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt ccatttctgc
   124081 taaagtaatt gtctctccag catttatcgg aacagaaggt gacccttttc tcccaattcc
   124141 tagatgactc gctgtcattg ccgctttccc tttttcacca tttttatttt ctggtgaaat
   124201 agttctactt tcctctccct tgtggagcca tatattattt aaacaattca ttttattcgt
   124261 cctctatcct ttcttcactt ttttgattat tcatatagat gatttgttgt tgtgcctccg
   124321 caataattgc tccaatatct aaattatcat ttcctaactg caaagcgatt gacattgcca
   124381 agggaagatt cattcctgca attacgtgca ctctgtcaga aatataggta aaaaactttt
   124441 gatttacact tcctccagcc atgtcagtta aaacaattat ttcgtctgta gtatccacta
   124501 actctttaat ttgttcatct atatcatcaa tttcttcgac atatgcacat aagctactaa
   124561 tttgattatg catacctgtt aaaaactcta gtgtctcttt aaaaccctca gctagtattg
   124621 aatggctagc aatgatcaat cttctcataa ttcaaatcct tttcctttac gctaatattc
   124681 caaatgcagc cataaccatc gcaaaaacaa taacgagtag aatcaaaatg gtcatactga
   124741 tttttttctt actgagtaga taatatgctg cagcagtaat aattactgga attaatgttg
   124801 gtaaaatttt gtccaacatt ggttggattt tcatactaac ttcaccaaaa gtgaatttaa
   124861 gtggcgttga aacactgata actgaagcaa ttaatgatcc aactactgtt agccccaata
   124921 ctgaagcagc ttcagttaaa taattaatct tttcaccgta ttcagtaaca atcttcattc
   124981 cctgcttata accaaattca aagcctctca ttcggaaaaa gaagattacc atgattaaaa
   125041 tgaaccataa aaagattcct acaggattat tttctaaagc catataagcc gcaattgatc
   125101 ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc aataccagca attggaccca
   125161 tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc taatccttgc ttttcctcca
   125221 ttgcaacaca agcacctaaa ataatggctg ccagccaagg ctgtgtattg taatactgaa
   125281 agtgattatt taaagcttcg atgtattcat catcatcttt gtataatttt cttaaaatag
   125341 gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg ataattaaag gtacaaactg
   125401 acatcaacca acgatagttc gctttcctaa gatccttttt cgttacttga tatttttcgc
   125461 tcattttatt catcctcccc aataaaccca acatcagaag ctcccgcaga agcagtgatt
   125521 tgtgcatctt tttcagctct atgatacaca ttaaatgcta aagcgaatcc tatgatcgcc
   125581 gctccaataa taggtacctt caaaaaagcg gctaagaaaa atccaacaag taaataacta
   125641 atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc aggcaacatt
   125701 tttccggcta atgttagccc atccataaac caattaggta aaacatccaa aacgtaatta
   125761 acagcagact gtcctaaagt aacggcaata aatactggaa ttgctgcaga taaaccaaag
   125821 aaaattggcc ccaaccacaa aacactattc attttcttaa attctcgttt attcgcatat
   125881 tcctgtgatt tgcgaacaac aaaaccattt aagattttga caattacatc tagttgcacc
   125941 cctaacatac ctacaggtag tcctattgct aatccggctt ctactccaag ctggctacca
   126001 aatgccgttg caataattgc gcacgttcca tagtccggta cagatgagcc accaaaaccg
   126061 gcaactccta atgacatcaa ttgaactgta ccgccaacta tcaaacctgt ttttatatca
   126121 cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa gatcatcgga
   126181 atcccataat aatcaacatg tttaataaat gctaatatag tcagcataat aatttgaaaa
   126241 atagtaaaat gcattatcct tactccttta tcctaatttt gattgaattt cctgtgcctt
   126301 atccgccgga acaaattgtg taacaatttc tacaccagaa tccaacaatt ttttatacgt
   126361 gctgatattt tcatctgatg cataggctct agaagatagc tgtaatgcat tatctttaac
   126421 aacagtaccg ccaacaatta gtcggtcaat tctaataccg ttatctacta aatgaaggat
   126481 tgtttccggc tctttcacga tgacaaatac tttttcctga tcgtatttcc cctttttaaa
   126541 attcgcaatc gctgtttctt cagtgataat tgatacagcc attccagcag gtctagctaa
   126601 acgcatactt gatttttgga tgtcgttatt tgcgacagaa tcatcgatca ccatcactct
   126661 gtttgcttta gacattggtg cccattgcgt gacaataatc ccatgcaata aacggttatc
   126721 aactctcgac ataacaatac tcataaatac ttcctccttt atctttcatt ttcttacaag
   126781 caattaccat gccaaaacaa aaagaacttg ataaatcaag ctctttttac ataatcataa
   126841 acgtatttaa cctcatgaat cgggatgtcg acactataat aatgttcaac gacactgaaa
   126901 gattgcttaa aatgtgtctg aaatttttga aattcctcac tcgtttcatt gaaataaatt
   126961 tcatcttcca aagtattttg ttttactaat cgttcaatta agcaactaat gtgaacatat
   127021 aatccgaaac acgtattatt atcaaatgaa attcctagat cattttgtaa gatgtatata
   127081 gcatctgaaa cccgttctaa taatttagtc ggatttaata tggtcaattg ctccattaaa
//
LOCUS       DANJJH010000002        82044 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000002 DANJJH010000000
VERSION     DANJJH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            355..1353
                     /locus_tag="PWS53_000118"
     CDS             355..1353
                     /locus_tag="PWS53_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870395.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1593..1676)
                     /locus_tag="PWS53_000119"
     tRNA            complement(1593..1676)
                     /locus_tag="PWS53_000119"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1640..1642),aa:Leu,seq:gag)
     gene            1731..1910
                     /locus_tag="PWS53_000120"
     CDS             1731..1910
                     /locus_tag="PWS53_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870396.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            2059..3276
                     /locus_tag="PWS53_000121"
     CDS             2059..3276
                     /locus_tag="PWS53_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0870397.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3460..4296
                     /locus_tag="PWS53_000122"
     CDS             3460..4296
                     /locus_tag="PWS53_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870398.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4372..5520
                     /gene="nagA"
                     /locus_tag="PWS53_000123"
     CDS             4372..5520
                     /gene="nagA"
                     /locus_tag="PWS53_000123"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL0870399.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5740..6798
                     /locus_tag="PWS53_000124"
     CDS             5740..6798
                     /locus_tag="PWS53_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL0870400.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6802..7836
                     /locus_tag="PWS53_000125"
     CDS             6802..7836
                     /locus_tag="PWS53_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL0870401.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7938..8654)
                     /locus_tag="PWS53_000126"
     CDS             complement(7938..8654)
                     /locus_tag="PWS53_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0870402.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8623..9408)
                     /locus_tag="PWS53_000127"
     CDS             complement(8623..9408)
                     /locus_tag="PWS53_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL0870403.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9399..10148)
                     /locus_tag="PWS53_000128"
     CDS             complement(9399..10148)
                     /locus_tag="PWS53_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0870404.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10446..10970
                     /locus_tag="PWS53_000129"
     CDS             10446..10970
                     /locus_tag="PWS53_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL0870405.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10986..12485
                     /locus_tag="PWS53_000130"
     CDS             10986..12485
                     /locus_tag="PWS53_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL0870406.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12573..13175)
                     /locus_tag="PWS53_000131"
     CDS             complement(12573..13175)
                     /locus_tag="PWS53_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0870407.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13279..14235
                     /locus_tag="PWS53_000132"
     CDS             13279..14235
                     /locus_tag="PWS53_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0870408.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14540..15496)
                     /locus_tag="PWS53_000133"
     CDS             complement(14540..15496)
                     /locus_tag="PWS53_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0870409.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15670..16230
                     /locus_tag="PWS53_000134"
     CDS             15670..16230
                     /locus_tag="PWS53_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870410.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16223..17047
                     /locus_tag="PWS53_000135"
     CDS             16223..17047
                     /locus_tag="PWS53_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL0870411.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            17057..17674
                     /locus_tag="PWS53_000136"
     CDS             17057..17674
                     /locus_tag="PWS53_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0870412.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17692..18375
                     /locus_tag="PWS53_000137"
     CDS             17692..18375
                     /locus_tag="PWS53_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL0870413.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            19058..20293
                     /locus_tag="PWS53_000138"
                     /pseudo
     CDS             19058..20293
                     /locus_tag="PWS53_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            20301..21188
                     /locus_tag="PWS53_000139"
     CDS             20301..21188
                     /locus_tag="PWS53_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870414.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            21205..22041
                     /locus_tag="PWS53_000140"
     CDS             21205..22041
                     /locus_tag="PWS53_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0870415.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            22044..22667
                     /locus_tag="PWS53_000141"
     CDS             22044..22667
                     /locus_tag="PWS53_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870416.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22664..23956
                     /locus_tag="PWS53_000142"
     CDS             22664..23956
                     /locus_tag="PWS53_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0870417.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24315..24412
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24528..25109
                     /locus_tag="PWS53_000143"
     CDS             24528..25109
                     /locus_tag="PWS53_000143"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL0870418.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            25112..26419
                     /locus_tag="PWS53_000144"
     CDS             25112..26419
                     /locus_tag="PWS53_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL0870419.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26712..27200
                     /gene="purE"
                     /locus_tag="PWS53_000145"
     CDS             26712..27200
                     /gene="purE"
                     /locus_tag="PWS53_000145"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL0870420.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27193..28317
                     /gene="purK"
                     /locus_tag="PWS53_000146"
     CDS             27193..28317
                     /gene="purK"
                     /locus_tag="PWS53_000146"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL0870421.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28330..29625
                     /gene="purB"
                     /locus_tag="PWS53_000147"
     CDS             28330..29625
                     /gene="purB"
                     /locus_tag="PWS53_000147"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL0870422.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29805..30182)
                     /locus_tag="PWS53_000148"
     CDS             complement(29805..30182)
                     /locus_tag="PWS53_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870423.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30438..31166
                     /locus_tag="PWS53_000149"
     CDS             30438..31166
                     /locus_tag="PWS53_000149"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL0870424.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31166..31420
                     /gene="purS"
                     /locus_tag="PWS53_000150"
     CDS             31166..31420
                     /gene="purS"
                     /locus_tag="PWS53_000150"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL0870425.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31422..32093
                     /gene="purQ"
                     /locus_tag="PWS53_000151"
     CDS             31422..32093
                     /gene="purQ"
                     /locus_tag="PWS53_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL0870426.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            32094..34316
                     /gene="purL"
                     /locus_tag="PWS53_000152"
     CDS             32094..34316
                     /gene="purL"
                     /locus_tag="PWS53_000152"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL0870427.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34301..35740
                     /locus_tag="PWS53_000153"
     CDS             34301..35740
                     /locus_tag="PWS53_000153"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL0870428.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35763..36815
                     /locus_tag="PWS53_000154"
     CDS             35763..36815
                     /locus_tag="PWS53_000154"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL0870429.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36812..37390
                     /gene="purN"
                     /locus_tag="PWS53_000155"
     CDS             36812..37390
                     /gene="purN"
                     /locus_tag="PWS53_000155"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL0870430.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37387..38922
                     /gene="purH"
                     /locus_tag="PWS53_000156"
     CDS             37387..38922
                     /gene="purH"
                     /locus_tag="PWS53_000156"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL0870431.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38938..40212
                     /gene="purD"
                     /locus_tag="PWS53_000157"
     CDS             38938..40212
                     /gene="purD"
                     /locus_tag="PWS53_000157"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL0870432.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40334..41248
                     /locus_tag="PWS53_000158"
     CDS             40334..41248
                     /locus_tag="PWS53_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0870433.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41381..42946)
                     /locus_tag="PWS53_000159"
     CDS             complement(41381..42946)
                     /locus_tag="PWS53_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0870434.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42939..44390)
                     /locus_tag="PWS53_000160"
     CDS             complement(42939..44390)
                     /locus_tag="PWS53_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0870435.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44404..45420)
                     /locus_tag="PWS53_000161"
     CDS             complement(44404..45420)
                     /locus_tag="PWS53_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0870436.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45640..46728
                     /locus_tag="PWS53_000162"
     CDS             45640..46728
                     /locus_tag="PWS53_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL0870437.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46782..48308
                     /locus_tag="PWS53_000163"
     CDS             46782..48308
                     /locus_tag="PWS53_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL0870438.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48310..49515
                     /locus_tag="PWS53_000164"
     CDS             48310..49515
                     /locus_tag="PWS53_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870439.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49772..50749
                     /gene="guaC"
                     /locus_tag="PWS53_000165"
     CDS             49772..50749
                     /gene="guaC"
                     /locus_tag="PWS53_000165"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL0870440.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            51013..52437
                     /locus_tag="PWS53_000166"
     CDS             51013..52437
                     /locus_tag="PWS53_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0870441.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52898..54007
                     /gene="pdhA"
                     /locus_tag="PWS53_000167"
     CDS             52898..54007
                     /gene="pdhA"
                     /locus_tag="PWS53_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL0870442.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            54010..54987
                     /locus_tag="PWS53_000168"
     CDS             54010..54987
                     /locus_tag="PWS53_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL0870443.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            55012..56655
                     /locus_tag="PWS53_000169"
     CDS             55012..56655
                     /locus_tag="PWS53_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL0870444.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56663..58069
                     /gene="lpdA"
                     /locus_tag="PWS53_000170"
     CDS             56663..58069
                     /gene="lpdA"
                     /locus_tag="PWS53_000170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL0870445.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            58231..58506
                     /locus_tag="PWS53_000171"
     CDS             58231..58506
                     /locus_tag="PWS53_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL0870446.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            58512..59279
                     /locus_tag="PWS53_000172"
     CDS             58512..59279
                     /locus_tag="PWS53_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL0870447.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            59447..61276
                     /gene="typA"
                     /locus_tag="PWS53_000173"
     CDS             59447..61276
                     /gene="typA"
                     /locus_tag="PWS53_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL0870448.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            61537..63309
                     /gene="recQ"
                     /locus_tag="PWS53_000174"
     CDS             61537..63309
                     /gene="recQ"
                     /locus_tag="PWS53_000174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL0870449.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            63423..63725
                     /locus_tag="PWS53_000175"
     CDS             63423..63725
                     /locus_tag="PWS53_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL0870450.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63728..64891
                     /locus_tag="PWS53_000176"
     CDS             63728..64891
                     /locus_tag="PWS53_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0870451.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64917..68345
                     /locus_tag="PWS53_000177"
     CDS             64917..68345
                     /locus_tag="PWS53_000177"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL0870452.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            68366..69490
                     /locus_tag="PWS53_000178"
     CDS             68366..69490
                     /locus_tag="PWS53_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870453.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            69480..69917
                     /locus_tag="PWS53_000179"
     CDS             69480..69917
                     /locus_tag="PWS53_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0870454.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69901..70239
                     /locus_tag="PWS53_000180"
     CDS             69901..70239
                     /locus_tag="PWS53_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL0870455.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            70358..70918
                     /gene="rsmD"
                     /locus_tag="PWS53_000181"
     CDS             70358..70918
                     /gene="rsmD"
                     /locus_tag="PWS53_000181"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL0870456.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70915..71406
                     /gene="coaD"
                     /locus_tag="PWS53_000182"
     CDS             70915..71406
                     /gene="coaD"
                     /locus_tag="PWS53_000182"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL0870457.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            71396..72439
                     /locus_tag="PWS53_000183"
     CDS             71396..72439
                     /locus_tag="PWS53_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL0870458.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72598..73326
                     /locus_tag="PWS53_000184"
     CDS             72598..73326
                     /locus_tag="PWS53_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL0870459.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            73442..73687
                     /locus_tag="PWS53_000185"
     CDS             73442..73687
                     /locus_tag="PWS53_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0870460.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73684..73902
                     /locus_tag="PWS53_000186"
     CDS             73684..73902
                     /locus_tag="PWS53_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL0870461.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            74038..74577
                     /locus_tag="PWS53_000187"
     CDS             74038..74577
                     /locus_tag="PWS53_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL0870462.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            74570..76843
                     /locus_tag="PWS53_000188"
     CDS             74570..76843
                     /locus_tag="PWS53_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL0870463.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76881..77927
                     /gene="holA"
                     /locus_tag="PWS53_000189"
     CDS             76881..77927
                     /gene="holA"
                     /locus_tag="PWS53_000189"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL0870464.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(78016..78270)
                     /locus_tag="PWS53_000190"
     CDS             complement(78016..78270)
                     /locus_tag="PWS53_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL0870465.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78670..78939)
                     /locus_tag="PWS53_000191"
     CDS             complement(78670..78939)
                     /locus_tag="PWS53_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870466.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(79409..79864)
                     /locus_tag="PWS53_000192"
     CDS             complement(79409..79864)
                     /locus_tag="PWS53_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL0870467.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(80002..81930)
                     /locus_tag="PWS53_000193"
     CDS             complement(80002..81930)
                     /locus_tag="PWS53_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0870468.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26603 a        14073 c        17732 g        23636 t
ORIGIN      
        1 aaaataacga ataacaaaga actgatcttg ttactaatag ggatttgggg tatcttttta
       61 ctctttaata tgtatatgcc gactatgaga gcggatgacg tagtttatgc aagtagatta
      121 gatgaacttg gctatttggg tgcttcgata gaacattata agacatggag ttcaagaatt
      181 attatagaac tattcttaat gttcttttct aaacacttta tgctatggaa attattaaat
      241 tctacgatta tgttaggaat tgtagtatta ctttgcaagt atgtttttga gaaaataaat
      301 gttaaaaatt tattattggt ttgctctata tactgtttaa taccattgac agtaatgggg
      361 gaaaccggtt ggagagcaac caccttgaac tatcagtggc cagtagcatt tagtttacta
      421 actttttatc cgttttttca actcttaaga ggagaagaaa taaatagaaa aatatattgg
      481 gtaagtattc ctttattgat atttttaact aatcaagaac aagtcaacgc ctgttttttt
      541 gttctaacaa gtatagttag tttatatttg attgtcaatg gacgatataa ttataagtta
      601 tcagtatttt caataataag cttagcagaa ttaattttct ctttgacaac tccagggaat
      661 gcattacgtg ctgcacatga aattaataaa tggtttcctg aatataaaaa tttcaacttt
      721 cttaataagt tagatttagg tatttcatca tttggaaagc cgtttttttt agacatgaat
      781 atcttatttt tattgctatt tttcttaatt ttccttctaa catataggaa atgtcaaaac
      841 tattatgtgc gtatattgac tgcattaccg tttttcttaa acctgattat ttattttggt
      901 aatacaatgg gtcaaagctt tacttacgtc aatggaaata aaagggcaat gatttggagt
      961 agtagtaacc tgaataatct ctttacagag ttaggaacga aactgtcact tttttatcca
     1021 ggtacttgga tagctacttt agtagtcttg gcattattac tatgtttaat tgttggaatt
     1081 tatttaagtt ttgacaataa gaaaacttct atttttttag ttattttgat gataatggga
     1141 ttttgttcaa ggttaattat gggattttct cccacagtat gggcttccgg aatgcggacg
     1201 tactatatac tttatgttgt tatcgccatc ttagtactaa tggcagttaa agagctaatg
     1261 aaaagcatga gtgtacaaaa aaatgaattt atgcaatttg gattaaccgt gcttggtatt
     1321 tgtaccttta taataacggt gataaacaga taatatacaa aaaatccctc gctttagtat
     1381 taggtgaggg attttactcg tttttcgatt aattgtttta acacttgcaa gtttctacga
     1441 gtagcttgtt tccgaccttc atatactcac ctcaggctgt ttacatgatt gtacgttctt
     1501 tttgtaaagg atacagtgat atgtatataa tggcataaag aaacgttcaa acaaaaaaac
     1561 tcgtgaagca aaattaactt cacgagttgt agtgcagatg atgggactcg aacccacacg
     1621 agctaacgct cacatgaccc tcaatcatgc ttgtctgcca attccaacac atctgcgtaa
     1681 taataacaca ctaatgatag cgaggtttta catagttgtc aagctcaaaa atgggaggac
     1741 ttattaagat ggagaaatat gacggagaat tttcaggact gggaatgatc ttaggtgtat
     1801 tgattggact tgcattcgga cgttttttgc ttggttttat gctaggaatc atttgtggaa
     1861 ttgcaatgga ttgggcagcg aatctatgga atgactatca tgacaattaa gttctcaacg
     1921 cttgtttgtg tttttttaat acttacgttt ttcctaaata agtggcaaaa ttttctagga
     1981 tggagtatga tattgaaata gtaaataaca aaaaatttgg aaagttctaa ctttttgaaa
     2041 tgagggattg gctaagtgat gagagaattt gaaaaatcca acaaattaga aggtgtcagt
     2101 tacgatgttc gcggtcccgt tctagaagaa gcggagcgga tgcaagaaga gggcattagt
     2161 attttaaaat tgaacacggg aaatcccgca ccttttggat ttgaagcacc aaacgaagtc
     2221 attcgtgatt tgatcatgaa tgcccgtgac tcagaaggct actcagattc caaggggatc
     2281 ttttctgcta gaaaagcaat tgagcaatat tgccaactga aacattttcc gaatgtgacg
     2341 atcaatgata tctacaccgg caacggagtg agcgaattga tcacgatgtg tatgcagggg
     2401 ttgctggaca atggagatga agtactggtt ccaatgccgg actatccgtt gtggacggct
     2461 tctattgcat tagctggagg cacacctgtt cattatattt gcgatgaaga ggcagaatgg
     2521 tatcctgata ttgatgatat caaatcaaaa ataacgagtc ggacaaaagc tattgttatc
     2581 attaatccta ataatcctac tggggcactt tatccaaaag aattattaga agaaatcgtt
     2641 gaagtagctc gacaaaataa cctgattatt tattccgacg aaatctatga tcgcttagtg
     2701 atggatggtc tagaacatat tccgattgct acattagcgc ctgatttgtt cgttgttacg
     2761 ctgaacggtc tatccaaatc tcatcgtgta gcaggatttc gagtaggctg gatggtccta
     2821 agtggagata agtcacacgt caaaggctat atagaagggc tgaatatgtt gtcctccatg
     2881 cgtttgtgtt ccaatgtatt gtctcagcaa attatccaaa ccgcgctagg cggttatcaa
     2941 agtgtcgatg agctgctcct gccaggcgga aggatctatg aacaaagaga atatatctat
     3001 aatgcaatca atgatattcc tggtctttca gccgtaaaac caaaagctgc tttttatata
     3061 ttcccaaaaa ttgatacaaa gcgattcaat attttggatg atgaaaaatt cgttttggac
     3121 tttttacatg aacatcatat tctacttgta catggtggtg gatttaattg gaaccagcca
     3181 gatcattttc gaatcgttta tcttcctaaa atggatgatt tgaaacaaac agctaagaaa
     3241 atgagagaat tcctagcaac ctatcgccaa aaatgatttt aaaaaccaaa tttttcagta
     3301 ggagggccac agacaaacac actgaggctc tctttctagt ggagttttct taaataacta
     3361 aagtatatta tttagttgca agtcttttct tgaagtttgc gacttcctaa agtaaaatac
     3421 gaatagaatg cgaatacgca tattttgggg aggatactta tggaaacgtt aatcgagtcc
     3481 tttggaacga tcattaaaga gattcgaaaa gaacaaaaaa tgacgcaaaa aatgctttcg
     3541 catgatattt gttcacaaag tgtattaagt cggatcgaaa ataatgagga actgccgaat
     3601 gtattggtga tgatgcagat ttgtcaacgg ctaggggtaa cgattgatca tgtgatgaat
     3661 ctctctcgaa aaactactcg gacaaacgat aaattatttg aactgttgga tagttgtttc
     3721 caaaaacgtg attatcggaa gttagaacaa gtactaaaat cttctgatat ttctgaaaat
     3781 ctgtatcaag caactgactt tcaacgctat tattattatc tcggtgtttg cgaattcacg
     3841 ttgcgtcaaa atatctctca gtccctttat tatttgaaag aagcattaag ctactctagc
     3901 caaaaagaaa gaatccatgt ttctgacaca gaaatacaac tgattagttg tatcggaaaa
     3961 atatatggtg tggcaggaaa aacggctgaa gcacgctact atttgagtcg aagcatccaa
     4021 ctgctgcatc aatcagctga tgaacgatca aaaagtgaat tttcgcaaat tttttataat
     4081 tatggaaatt tcttattcca tcaaaatgaa atcgacgaag cgttagaaca ggtgaatcaa
     4141 ggaatctatt gggctcaaga gaagaatagc tactattacc tcaatgattt atttgtgctt
     4201 aaaagcttaa tatacaagcg taaagaagaa ccagaaagag cagcattcta tgaaggctta
     4261 gcacaagctg tgaaacaaat ttcaaaaaat ttgtaaatgg tgtagaccat tcttgacttc
     4321 ttctctaaat aagagtactt tagaggtaga aaaacaaagg gagagaatag aatgagaact
     4381 tttatctatg ctgacaaatt ctttttagcg tcaggtgtca aacatgcagg ctatcttgaa
     4441 attaccgatg gactttttgg tgaatacacc aaagaactac ctgagggaga agtcaacatc
     4501 attgaccaaa gcggcaaatg gattgctcct ggtttggtag atacacatat tcacggctat
     4561 atgaatcatg atgtaatgga taatgatgca gaaggcatca aagtgatgtc tgaaggtctg
     4621 ttatcttgtg gagtgacttc atttttacca acaacattga cgtcaagcaa agaacgttta
     4681 aaagatgtag ctgagacaat cggcaaaatg tatcaggaag caccaggagc taaaatcaaa
     4741 ggaatctatt ttgaaggtcc tttcttcaca gaagaacaca aaggggcaca aaacccaagt
     4801 tatttcgggg atcctgattt ggatactttc catgaatggc aagaagcttc aggtggttta
     4861 atcaaaaaaa ttgctttagc accggaacgt caaggtgtag aagagtttgt caaacaagtg
     4921 actgaagaag gagtagttgt ttctctagga cacagtaatg ctacactaga agaagcgcaa
     4981 aaagctgttg aagcaggcgc aagtgtattt gtccatgctt tcaatggtat gcgtggattg
     5041 aatcatcgcg aaccaggtat ggtgggtgcg ttattatctt tagaacatgt cttttctgaa
     5101 ttgatttgtg atggccatca tgtacatcca aatgctgcgg gaatcctaat ggaaaaagca
     5161 ggacatgatc atgtcgcatt aattacagac tgtatgatgg ccggtgggat gcctgatgga
     5221 aattacaatt taggcgaatt tccagtagta gtaaaagaag gaacagcacg tctagaatct
     5281 ggcaacttag ctggaagtat attgaaattg aaagaagcaa ttaaaaatgt tgtagactgg
     5341 ggattagcaa caccagaaca agcagtcatg atggctagtc tgattccagc aatcagctgt
     5401 aaaatcgatg atacatgtgg aaaaatcgct caaggacgcg atgcagactt tatcgtattg
     5461 aaccctgata tgacattgga agcaacttat ctagatggcg tggaacgttt ccacgcataa
     5521 tataatttaa gttaacctta aaactaaact tcttaagttt ggttttaagg tttttttgtg
     5581 ttctagtaaa aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat
     5641 aagtaacaat gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt
     5701 ttttattgtg aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt
     5761 cgatcgactt tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata
     5821 ttattagggt ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta
     5881 tcgaaagtcg tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat
     5941 atcaatacag aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat
     6001 attattctta ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca
     6061 acgttgcgtg tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt
     6121 gcgatttatg gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc
     6181 tttttgtatt cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc
     6241 aaagcgatgt tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg
     6301 ttccaagggt atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta
     6361 gcgtttttgc gaattgacca gactgcaccg tggctatttc agccgatgga ttatttagct
     6421 ggtttaagtt caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc
     6481 aaaaaagctg catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta
     6541 cctgcattga atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat
     6601 tttgaagcac tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca
     6661 gcctatgcta tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca
     6721 actatttttg ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc
     6781 ataggtttat tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt
     6841 cgtccgaacg agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt
     6901 tatgaagaac tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg
     6961 ttgaggggaa attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt
     7021 cgttatgttt ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat
     7081 tttggtatga cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct
     7141 ttgacttttg ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc
     7201 aaagattttc gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg
     7261 atcataggaa caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc
     7321 aaagtgatcg gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac
     7381 tcgcttgaag agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca
     7441 ggtgaaaacg atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc
     7501 ttgattaata cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa
     7561 agcaatcatt tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc
     7621 cgatccttca agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg
     7681 taccctagag ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat
     7741 atgatttcga caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat
     7801 gaggtatctc ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa
     7861 aatgtttaaa aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta
     7921 tgggataatt cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat
     7981 tcataacagg ttagcggctg gccgatttcc aaatatttga ttctttcgtt caatatatct
     8041 tgttctacta aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga
     8101 gccagttctt ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt
     8161 aaagtcagtt ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga
     8221 gtggtgtggt tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt
     8281 gtctgagaga aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt
     8341 ttgatcaaat aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct
     8401 gcagcagtga tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa
     8461 tggataccac tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt
     8521 ctgaaatatt tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg
     8581 atgctttcca caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg
     8641 acatttgtca tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc
     8701 atttgtttgt ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag
     8761 attcaatcca aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc
     8821 tgagatttct tctgatatcc cgacaccgcc atcgcagacg gtcacgatga atacttcagc
     8881 ttctgtgtca tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac
     8941 ggcttctttt gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc
     9001 atccatctcc attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc
     9061 tttggcttcg tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact
     9121 tgagacttgg ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac
     9181 ttcttcatac ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc
     9241 atgggtttga gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa
     9301 tttcattact tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct
     9361 aaaaagaggt gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg
     9421 aacggtcgtt tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat
     9481 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
     9541 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
     9601 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
     9661 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
     9721 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
     9781 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
     9841 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
     9901 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
     9961 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
    10021 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
    10081 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
    10141 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
    10201 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
    10261 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
    10321 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
    10381 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
    10441 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
    10501 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
    10561 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
    10621 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
    10681 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
    10741 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
    10801 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
    10861 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
    10921 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
    10981 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
    11041 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
    11101 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
    11161 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
    11221 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
    11281 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
    11341 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
    11401 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
    11461 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
    11521 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
    11581 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
    11641 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
    11701 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
    11761 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
    11821 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
    11881 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
    11941 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
    12001 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
    12061 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
    12121 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
    12181 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
    12241 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
    12301 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
    12361 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
    12421 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
    12481 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
    12541 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
    12601 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
    12661 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
    12721 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
    12781 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
    12841 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
    12901 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
    12961 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
    13021 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
    13081 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
    13141 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
    13201 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
    13261 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
    13321 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
    13381 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
    13441 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
    13501 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
    13561 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
    13621 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
    13681 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
    13741 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
    13801 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
    13861 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
    13921 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
    13981 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
    14041 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
    14101 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
    14161 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
    14221 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
    14281 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
    14341 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
    14401 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
    14461 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
    14521 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
    14581 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
    14641 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
    14701 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
    14761 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
    14821 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
    14881 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
    14941 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
    15001 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
    15061 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
    15121 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
    15181 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
    15241 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
    15301 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
    15361 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
    15421 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
    15481 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
    15541 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
    15601 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
    15661 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
    15721 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
    15781 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
    15841 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
    15901 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
    15961 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
    16021 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
    16081 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
    16141 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
    16201 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
    16261 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
    16321 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
    16381 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
    16441 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
    16501 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
    16561 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
    16621 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
    16681 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
    16741 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
    16801 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
    16861 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
    16921 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
    16981 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
    17041 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
    17101 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
    17161 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
    17221 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
    17281 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
    17341 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
    17401 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
    17461 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
    17521 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
    17581 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
    17641 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
    17701 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
    17761 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
    17821 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
    17881 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
    17941 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
    18001 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
    18061 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
    18121 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
    18181 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
    18241 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
    18301 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
    18361 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
    18421 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
    18481 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
    18541 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
    18601 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
    18661 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
    18721 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
    18781 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
    18841 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
    18901 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
    18961 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
    19021 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
    19081 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
    19141 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
    19201 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
    19261 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
    19321 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
    19381 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
    19441 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    19501 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    19561 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    19621 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    19681 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    19741 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    19801 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    19861 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    19921 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    19981 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    20041 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    20101 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    20161 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    20221 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    20281 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    20341 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    20401 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    20461 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    20521 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    20581 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    20641 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    20701 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    20761 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    20821 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    20881 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    20941 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    21001 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    21061 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    21121 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    21181 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    21241 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    21301 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    21361 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    21421 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    21481 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    21541 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    21601 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    21661 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    21721 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    21781 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    21841 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    21901 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    21961 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    22021 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    22081 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    22141 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    22201 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    22261 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    22321 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    22381 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    22441 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    22501 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    22561 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    22621 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    22681 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    22741 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    22801 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    22861 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    22921 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    22981 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    23041 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    23101 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    23161 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    23221 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    23281 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    23341 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    23401 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    23461 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    23521 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    23581 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    23641 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    23701 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    23761 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    23821 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    23881 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    23941 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    24001 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    24061 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    24121 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    24181 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    24241 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    24301 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    24361 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    24421 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    24481 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    24541 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    24601 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    24661 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    24721 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    24781 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    24841 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    24901 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    24961 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    25021 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    25081 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    25141 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    25201 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    25261 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    25321 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    25381 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    25441 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    25501 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    25561 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    25621 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    25681 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    25741 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    25801 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    25861 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    25921 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    25981 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    26041 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    26101 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    26161 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    26221 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    26281 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    26341 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    26401 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    26461 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    26521 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    26581 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    26641 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    26701 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    26761 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    26821 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    26881 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    26941 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    27001 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    27061 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    27121 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    27181 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    27241 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    27301 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    27361 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    27421 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    27481 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    27541 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    27601 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    27661 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    27721 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    27781 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    27841 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    27901 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    27961 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    28021 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    28081 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    28141 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    28201 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    28261 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    28321 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    28381 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    28441 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    28501 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    28561 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    28621 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    28681 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    28741 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    28801 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    28861 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    28921 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    28981 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    29041 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    29101 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    29161 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    29221 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    29281 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    29341 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    29401 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    29461 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    29521 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    29581 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    29641 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    29701 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    29761 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    29821 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    29881 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    29941 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    30001 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    30061 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    30121 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    30181 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    30241 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    30301 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    30361 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    30421 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    30481 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    30541 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    30601 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    30661 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    30721 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    30781 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    30841 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    30901 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    30961 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    31021 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    31081 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    31141 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    31201 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    31261 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    31321 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    31381 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    31441 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    31501 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    31561 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    31621 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    31681 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    31741 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    31801 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    31861 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    31921 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    31981 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    32041 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    32101 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    32161 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    32221 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    32281 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    32341 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    32401 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    32461 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    32521 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    32581 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    32641 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    32701 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    32761 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    32821 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    32881 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    32941 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    33001 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    33061 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    33121 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    33181 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    33241 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    33301 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    33361 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    33421 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    33481 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    33541 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    33601 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    33661 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    33721 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    33781 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    33841 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    33901 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    33961 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    34021 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    34081 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    34141 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    34201 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    34261 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    34321 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    34381 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    34441 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    34501 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    34561 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    34621 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    34681 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    34741 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    34801 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    34861 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    34921 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    34981 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    35041 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    35101 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    35161 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    35221 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    35281 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    35341 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    35401 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    35461 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    35521 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    35581 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    35641 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    35701 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    35761 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    35821 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    35881 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    35941 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    36001 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    36061 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    36121 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    36181 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    36241 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    36301 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    36361 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    36421 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    36481 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    36541 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    36601 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    36661 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    36721 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    36781 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    36841 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    36901 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    36961 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    37021 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    37081 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    37141 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    37201 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    37261 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    37321 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    37381 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    37441 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    37501 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    37561 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    37621 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    37681 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    37741 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    37801 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    37861 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    37921 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    37981 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    38041 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    38101 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    38161 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    38221 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    38281 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    38341 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    38401 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    38461 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    38521 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    38581 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    38641 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    38701 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    38761 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    38821 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    38881 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    38941 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    39001 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    39061 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    39121 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    39181 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    39241 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    39301 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    39361 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    39421 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    39481 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    39541 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    39601 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    39661 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    39721 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    39781 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    39841 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    39901 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    39961 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    40021 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    40081 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    40141 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    40201 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    40261 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    40321 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    40381 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    40441 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    40501 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    40561 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    40621 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    40681 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    40741 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    40801 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    40861 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    40921 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    40981 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    41041 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    41101 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    41161 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    41221 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    41281 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    41341 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    41401 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    41461 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    41521 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    41581 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    41641 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    41701 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    41761 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    41821 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    41881 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    41941 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    42001 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    42061 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    42121 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    42181 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    42241 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    42301 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    42361 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    42421 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    42481 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    42541 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    42601 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    42661 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    42721 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    42781 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    42841 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    42901 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    42961 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    43021 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    43081 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    43141 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    43201 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    43261 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    43321 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    43381 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    43441 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    43501 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    43561 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    43621 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    43681 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    43741 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    43801 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    43861 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    43921 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    43981 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    44041 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    44101 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    44161 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    44221 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    44281 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    44341 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    44401 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    44461 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    44521 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    44581 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    44641 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    44701 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    44761 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    44821 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    44881 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    44941 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    45001 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    45061 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    45121 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    45181 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    45241 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    45301 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    45361 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    45421 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    45481 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    45541 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    45601 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    45661 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    45721 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    45781 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    45841 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    45901 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    45961 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    46021 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    46081 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    46141 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    46201 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    46261 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    46321 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    46381 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    46441 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    46501 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    46561 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    46621 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    46681 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    46741 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    46801 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    46861 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    46921 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    46981 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    47041 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    47101 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    47161 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    47221 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    47281 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    47341 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    47401 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    47461 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    47521 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    47581 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    47641 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    47701 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    47761 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    47821 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    47881 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    47941 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    48001 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    48061 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    48121 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    48181 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    48241 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    48301 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    48361 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    48421 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    48481 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    48541 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    48601 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    48661 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    48721 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    48781 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    48841 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    48901 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    48961 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    49021 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    49081 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    49141 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    49201 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    49261 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    49321 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    49381 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    49441 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    49501 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    49561 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    49621 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    49681 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    49741 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    49801 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    49861 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    49921 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    49981 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    50041 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    50101 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    50161 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    50221 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    50281 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    50341 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    50401 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    50461 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    50521 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    50581 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    50641 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    50701 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    50761 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    50821 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    50881 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    50941 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    51001 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    51061 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    51121 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    51181 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    51241 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    51301 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    51361 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    51421 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    51481 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    51541 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    51601 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    51661 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    51721 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    51781 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    51841 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    51901 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    51961 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    52021 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    52081 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    52141 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    52201 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    52261 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    52321 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    52381 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    52441 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    52501 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    52561 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    52621 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    52681 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    52741 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    52801 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    52861 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    52921 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    52981 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    53041 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    53101 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    53161 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    53221 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    53281 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    53341 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    53401 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    53461 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    53521 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    53581 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    53641 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    53701 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    53761 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    53821 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    53881 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    53941 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    54001 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    54061 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    54121 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    54181 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    54241 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    54301 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    54361 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    54421 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    54481 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    54541 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    54601 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    54661 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    54721 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    54781 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    54841 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    54901 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    54961 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    55021 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    55081 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    55141 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    55201 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    55261 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    55321 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    55381 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    55441 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    55501 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    55561 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    55621 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    55681 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    55741 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    55801 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    55861 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    55921 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    55981 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    56041 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    56101 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    56161 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    56221 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    56281 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    56341 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    56401 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    56461 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    56521 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    56581 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    56641 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    56701 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    56761 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    56821 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    56881 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    56941 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    57001 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    57061 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    57121 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    57181 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    57241 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    57301 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    57361 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    57421 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    57481 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    57541 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    57601 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    57661 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    57721 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    57781 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    57841 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    57901 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    57961 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    58021 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    58081 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    58141 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    58201 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    58261 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    58321 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    58381 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    58441 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    58501 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    58561 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    58621 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    58681 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    58741 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    58801 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    58861 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    58921 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    58981 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    59041 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    59101 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    59161 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    59221 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    59281 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    59341 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    59401 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    59461 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    59521 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    59581 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    59641 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    59701 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    59761 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    59821 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    59881 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    59941 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    60001 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    60061 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    60121 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    60181 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    60241 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    60301 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    60361 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    60421 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    60481 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    60541 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    60601 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    60661 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    60721 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    60781 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    60841 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    60901 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    60961 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    61021 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    61081 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    61141 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    61201 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    61261 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    61321 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    61381 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    61441 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    61501 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    61561 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    61621 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    61681 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    61741 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    61801 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    61861 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    61921 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    61981 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    62041 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    62101 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    62161 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    62221 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    62281 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    62341 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    62401 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    62461 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    62521 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    62581 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    62641 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    62701 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    62761 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    62821 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    62881 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    62941 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    63001 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    63061 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    63121 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    63181 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    63241 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    63301 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    63361 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    63421 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    63481 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    63541 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    63601 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    63661 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    63721 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    63781 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    63841 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    63901 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    63961 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    64021 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    64081 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    64141 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    64201 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    64261 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    64321 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    64381 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    64441 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    64501 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    64561 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    64621 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    64681 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    64741 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    64801 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    64861 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    64921 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    64981 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    65041 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    65101 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    65161 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    65221 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    65281 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    65341 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    65401 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    65461 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    65521 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    65581 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    65641 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    65701 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    65761 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    65821 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    65881 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    65941 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    66001 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    66061 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    66121 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    66181 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    66241 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    66301 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    66361 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    66421 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    66481 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    66541 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    66601 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    66661 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    66721 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    66781 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    66841 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    66901 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    66961 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    67021 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    67081 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    67141 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    67201 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    67261 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    67321 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    67381 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    67441 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    67501 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    67561 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    67621 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    67681 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    67741 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    67801 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    67861 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    67921 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    67981 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    68041 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    68101 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    68161 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    68221 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    68281 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    68341 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    68401 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    68461 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    68521 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    68581 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    68641 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    68701 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    68761 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    68821 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    68881 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    68941 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    69001 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    69061 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    69121 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    69181 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    69241 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    69301 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    69361 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    69421 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    69481 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    69541 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    69601 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    69661 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    69721 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    69781 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    69841 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    69901 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    69961 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    70021 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    70081 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    70141 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    70201 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    70261 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    70321 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    70381 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    70441 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    70501 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    70561 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    70621 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    70681 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    70741 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    70801 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    70861 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    70921 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    70981 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    71041 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    71101 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    71161 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    71221 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    71281 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    71341 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    71401 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    71461 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    71521 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    71581 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    71641 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    71701 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    71761 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    71821 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    71881 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    71941 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    72001 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    72061 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    72121 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    72181 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    72241 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    72301 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    72361 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    72421 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    72481 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    72541 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    72601 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    72661 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    72721 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    72781 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    72841 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    72901 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    72961 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    73021 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    73081 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    73141 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    73201 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    73261 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    73321 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    73381 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    73441 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    73501 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    73561 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    73621 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    73681 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    73741 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    73801 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    73861 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    73921 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    73981 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    74041 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    74101 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    74161 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    74221 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    74281 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    74341 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    74401 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    74461 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    74521 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    74581 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    74641 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    74701 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    74761 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    74821 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    74881 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    74941 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    75001 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    75061 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    75121 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    75181 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    75241 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    75301 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    75361 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    75421 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    75481 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    75541 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    75601 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    75661 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    75721 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    75781 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    75841 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    75901 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    75961 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    76021 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    76081 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    76141 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    76201 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    76261 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    76321 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    76381 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    76441 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    76501 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    76561 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    76621 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    76681 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    76741 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    76801 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    76861 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    76921 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    76981 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    77041 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    77101 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    77161 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    77221 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    77281 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    77341 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    77401 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    77461 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    77521 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    77581 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    77641 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    77701 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    77761 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    77821 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    77881 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    77941 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    78001 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    78061 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    78121 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    78181 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    78241 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    78301 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    78361 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    78421 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    78481 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    78541 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    78601 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    78661 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    78721 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    78781 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    78841 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    78901 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    78961 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    79021 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    79081 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    79141 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    79201 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    79261 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    79321 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    79381 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    79441 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    79501 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    79561 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    79621 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    79681 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    79741 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    79801 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    79861 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    79921 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    79981 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    80041 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    80101 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    80161 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    80221 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    80281 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    80341 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    80401 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    80461 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    80521 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    80581 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    80641 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    80701 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    80761 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    80821 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    80881 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    80941 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    81001 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    81061 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    81121 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    81181 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    81241 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    81301 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    81361 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    81421 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    81481 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    81541 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    81601 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    81661 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    81721 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    81781 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    81841 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    81901 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    81961 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
    82021 atttggtgat taagcttgat gttg
//
LOCUS       DANJJH010000003        78978 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000003 DANJJH010000000
VERSION     DANJJH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            115..1089
                     /locus_tag="PWS53_000194"
     CDS             115..1089
                     /locus_tag="PWS53_000194"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0870469.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1259..2659
                     /locus_tag="PWS53_000195"
     CDS             1259..2659
                     /locus_tag="PWS53_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL0870470.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2737..3459)
                     /locus_tag="PWS53_000196"
     CDS             complement(2737..3459)
                     /locus_tag="PWS53_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0870471.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3538..4044)
                     /locus_tag="PWS53_000197"
     CDS             complement(3538..4044)
                     /locus_tag="PWS53_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0870472.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4167..5966
                     /locus_tag="PWS53_000198"
     CDS             4167..5966
                     /locus_tag="PWS53_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL0870473.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6034..7608)
                     /locus_tag="PWS53_000199"
     CDS             complement(6034..7608)
                     /locus_tag="PWS53_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0870474.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(7863..8372)
                     /gene="mreD"
                     /locus_tag="PWS53_000200"
     CDS             complement(7863..8372)
                     /gene="mreD"
                     /locus_tag="PWS53_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL0870475.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8376..9230)
                     /gene="mreC"
                     /locus_tag="PWS53_000201"
     CDS             complement(8376..9230)
                     /gene="mreC"
                     /locus_tag="PWS53_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL0870476.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(9385..10023)
                     /locus_tag="PWS53_000202"
     CDS             complement(9385..10023)
                     /locus_tag="PWS53_000202"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL0870477.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(10024..10674)
                     /locus_tag="PWS53_000203"
     CDS             complement(10024..10674)
                     /locus_tag="PWS53_000203"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL0870478.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(10687..11586)
                     /gene="rsgA"
                     /locus_tag="PWS53_000204"
     CDS             complement(10687..11586)
                     /gene="rsgA"
                     /locus_tag="PWS53_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL0870479.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(11751..13820)
                     /gene="pknB"
                     /locus_tag="PWS53_000205"
     CDS             complement(11751..13820)
                     /gene="pknB"
                     /locus_tag="PWS53_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL0870480.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(13817..14557)
                     /locus_tag="PWS53_000206"
     CDS             complement(13817..14557)
                     /locus_tag="PWS53_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL0870481.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(14570..15928)
                     /gene="rsmB"
                     /locus_tag="PWS53_000207"
     CDS             complement(14570..15928)
                     /gene="rsmB"
                     /locus_tag="PWS53_000207"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL0870482.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(15928..16866)
                     /locus_tag="PWS53_000208"
     CDS             complement(15928..16866)
                     /locus_tag="PWS53_000208"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL0870483.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(16859..17350)
                     /gene="def"
                     /locus_tag="PWS53_000209"
     CDS             complement(16859..17350)
                     /gene="def"
                     /locus_tag="PWS53_000209"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0870484.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(17365..19773)
                     /gene="priA"
                     /locus_tag="PWS53_000210"
     CDS             complement(17365..19773)
                     /gene="priA"
                     /locus_tag="PWS53_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL0870485.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(20026..20331)
                     /locus_tag="PWS53_000211"
     CDS             complement(20026..20331)
                     /locus_tag="PWS53_000211"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0870486.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(20336..20950)
                     /gene="gmk"
                     /locus_tag="PWS53_000212"
     CDS             complement(20336..20950)
                     /gene="gmk"
                     /locus_tag="PWS53_000212"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL0870487.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(21437..21721)
                     /locus_tag="PWS53_000213"
     CDS             complement(21437..21721)
                     /locus_tag="PWS53_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870488.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(21739..22629)
                     /locus_tag="PWS53_000214"
     CDS             complement(21739..22629)
                     /locus_tag="PWS53_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL0870489.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(22649..22867)
                     /locus_tag="PWS53_000215"
     CDS             complement(22649..22867)
                     /locus_tag="PWS53_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL0870490.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(22837..24537)
                     /locus_tag="PWS53_000216"
     CDS             complement(22837..24537)
                     /locus_tag="PWS53_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL0870491.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            24804..25577
                     /locus_tag="PWS53_000217"
     CDS             24804..25577
                     /locus_tag="PWS53_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870492.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(25658..26227)
                     /gene="thiT"
                     /locus_tag="PWS53_000218"
     CDS             complement(25658..26227)
                     /gene="thiT"
                     /locus_tag="PWS53_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL0870493.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(26293..26395)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(26401..27372)
                     /locus_tag="PWS53_000219"
     CDS             complement(26401..27372)
                     /locus_tag="PWS53_000219"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0870494.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(27466..28839)
                     /gene="glmU"
                     /locus_tag="PWS53_000220"
     CDS             complement(27466..28839)
                     /gene="glmU"
                     /locus_tag="PWS53_000220"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL0870495.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(29342..30160)
                     /gene="purR"
                     /locus_tag="PWS53_000221"
     CDS             complement(29342..30160)
                     /gene="purR"
                     /locus_tag="PWS53_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL0870496.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(30327..31151)
                     /locus_tag="PWS53_000222"
     CDS             complement(30327..31151)
                     /locus_tag="PWS53_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0870497.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(31124..31804)
                     /locus_tag="PWS53_000223"
     CDS             complement(31124..31804)
                     /locus_tag="PWS53_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0870498.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(31827..32765)
                     /locus_tag="PWS53_000224"
     CDS             complement(31827..32765)
                     /locus_tag="PWS53_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL0870499.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(33010..33873)
                     /locus_tag="PWS53_000225"
     CDS             complement(33010..33873)
                     /locus_tag="PWS53_000225"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL0870500.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(34015..36588)
                     /gene="mprF"
                     /locus_tag="PWS53_000226"
     CDS             complement(34015..36588)
                     /gene="mprF"
                     /locus_tag="PWS53_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0870501.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            36729..37535
                     /gene="proC"
                     /locus_tag="PWS53_000227"
     CDS             36729..37535
                     /gene="proC"
                     /locus_tag="PWS53_000227"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL0870502.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(37614..39170)
                     /gene="rny"
                     /locus_tag="PWS53_000228"
     CDS             complement(37614..39170)
                     /gene="rny"
                     /locus_tag="PWS53_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL0870503.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(39581..40630)
                     /gene="recA"
                     /locus_tag="PWS53_000229"
     CDS             complement(39581..40630)
                     /gene="recA"
                     /locus_tag="PWS53_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL0870504.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(40739..41980)
                     /locus_tag="PWS53_000230"
     CDS             complement(40739..41980)
                     /locus_tag="PWS53_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL0870505.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(42220..42798)
                     /gene="pgsA"
                     /locus_tag="PWS53_000231"
     CDS             complement(42220..42798)
                     /gene="pgsA"
                     /locus_tag="PWS53_000231"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL0870506.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(43007..43930)
                     /locus_tag="PWS53_000232"
     CDS             complement(43007..43930)
                     /locus_tag="PWS53_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870507.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(44669..45955)
                     /locus_tag="PWS53_000233"
     CDS             complement(44669..45955)
                     /locus_tag="PWS53_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0870508.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(45948..47216)
                     /locus_tag="PWS53_000234"
     CDS             complement(45948..47216)
                     /locus_tag="PWS53_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0870509.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            47468..47929
                     /gene="mscL"
                     /locus_tag="PWS53_000235"
     CDS             47468..47929
                     /gene="mscL"
                     /locus_tag="PWS53_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL0870510.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(47989..48435)
                     /locus_tag="PWS53_000236"
     CDS             complement(47989..48435)
                     /locus_tag="PWS53_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870511.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(48528..48773)
                     /locus_tag="PWS53_000237"
     CDS             complement(48528..48773)
                     /locus_tag="PWS53_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL0870512.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(48888..49466)
                     /locus_tag="PWS53_000238"
     CDS             complement(48888..49466)
                     /locus_tag="PWS53_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0870513.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(49543..50073)
                     /locus_tag="PWS53_000239"
     CDS             complement(49543..50073)
                     /locus_tag="PWS53_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL0870514.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(50077..50937)
                     /gene="rlmB"
                     /locus_tag="PWS53_000240"
     CDS             complement(50077..50937)
                     /gene="rlmB"
                     /locus_tag="PWS53_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL0870515.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(50930..51325)
                     /locus_tag="PWS53_000241"
     CDS             complement(50930..51325)
                     /locus_tag="PWS53_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL0870516.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(51322..52734)
                     /locus_tag="PWS53_000242"
     CDS             complement(51322..52734)
                     /locus_tag="PWS53_000242"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL0870517.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(52731..53267)
                     /gene="cysE"
                     /locus_tag="PWS53_000243"
     CDS             complement(52731..53267)
                     /gene="cysE"
                     /locus_tag="PWS53_000243"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL0870518.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(53353..53592)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(53589..55049)
                     /locus_tag="PWS53_000244"
     CDS             complement(53589..55049)
                     /locus_tag="PWS53_000244"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL0870519.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(55219..56376)
                     /locus_tag="PWS53_000245"
     CDS             complement(55219..56376)
                     /locus_tag="PWS53_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL0870520.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(56410..57780)
                     /gene="radA"
                     /locus_tag="PWS53_000246"
     CDS             complement(56410..57780)
                     /gene="radA"
                     /locus_tag="PWS53_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL0870521.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(57843..58370)
                     /locus_tag="PWS53_000247"
     CDS             complement(57843..58370)
                     /locus_tag="PWS53_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL0870522.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            58600..59406
                     /gene="proB"
                     /locus_tag="PWS53_000248"
     CDS             58600..59406
                     /gene="proB"
                     /locus_tag="PWS53_000248"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL0870523.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            59403..60650
                     /locus_tag="PWS53_000249"
     CDS             59403..60650
                     /locus_tag="PWS53_000249"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL0870524.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            60726..61070
                     /locus_tag="PWS53_000250"
     CDS             60726..61070
                     /locus_tag="PWS53_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870525.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(61187..61513)
                     /locus_tag="PWS53_000251"
     CDS             complement(61187..61513)
                     /locus_tag="PWS53_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870526.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(62128..63330)
                     /locus_tag="PWS53_000252"
     CDS             complement(62128..63330)
                     /locus_tag="PWS53_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0870527.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            63447..63962
                     /locus_tag="PWS53_000253"
     CDS             63447..63962
                     /locus_tag="PWS53_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0870528.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(64161..64607)
                     /locus_tag="PWS53_000254"
     CDS             complement(64161..64607)
                     /locus_tag="PWS53_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0870529.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(64654..66132)
                     /gene="msr(C)"
                     /locus_tag="PWS53_000255"
     CDS             complement(64654..66132)
                     /gene="msr(C)"
                     /locus_tag="PWS53_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0870530.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(66486..67115)
                     /locus_tag="PWS53_000256"
     CDS             complement(66486..67115)
                     /locus_tag="PWS53_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0870531.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(67099..67563)
                     /locus_tag="PWS53_000257"
     CDS             complement(67099..67563)
                     /locus_tag="PWS53_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0870532.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(68050..69318)
                     /locus_tag="PWS53_000258"
     CDS             complement(68050..69318)
                     /locus_tag="PWS53_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0870533.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(69549..70286)
                     /locus_tag="PWS53_000259"
     CDS             complement(69549..70286)
                     /locus_tag="PWS53_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0870534.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(70353..70922)
                     /locus_tag="PWS53_000260"
     CDS             complement(70353..70922)
                     /locus_tag="PWS53_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0870535.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            71050..71922
                     /locus_tag="PWS53_000261"
     CDS             71050..71922
                     /locus_tag="PWS53_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0870536.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            72264..73136
                     /locus_tag="PWS53_000262"
     CDS             72264..73136
                     /locus_tag="PWS53_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0870537.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            73133..75091
                     /locus_tag="PWS53_000263"
     CDS             73133..75091
                     /locus_tag="PWS53_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0870538.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(75423..76274)
                     /locus_tag="PWS53_000264"
     CDS             complement(75423..76274)
                     /locus_tag="PWS53_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0870539.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            76397..76864
                     /locus_tag="PWS53_000265"
     CDS             76397..76864
                     /locus_tag="PWS53_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL0870540.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(77127..77609)
                     /locus_tag="PWS53_000266"
     CDS             complement(77127..77609)
                     /locus_tag="PWS53_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL0870541.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(77709..78572)
                     /locus_tag="PWS53_000267"
     CDS             complement(77709..78572)
                     /locus_tag="PWS53_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0870542.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        22362 a        16858 c        14229 g        25529 t
ORIGIN      
        1 tataatacaa ccttgttatc tgttatattt tatagccaaa tagcgtgaca tggggcttga
       61 gaaatgttag cataagcatg ggaaaaaaca aatcaatgga aggattgata ctaaatgact
      121 gcagctgcag aaaaaaaaga tcatcaaaaa gttattcttg ttggagacgg cgccgtaggt
      181 tctagctatg cctttgctct agttactcaa aatatcgctc aagaagtagg tattgtggat
      241 atcaatactg ctaaaactga aggagacgca attgacttgt ctcacgcttt ggcatttact
      301 tcacctaaaa aaatctattc tgctacttac gcagacgcac atgatgctga cttagttgtt
      361 attacagctg gcgcaccaca aaaaccaggt gaaactcgtt tagacttagt aaacaaaaac
      421 ctaaaaatca accgcgatgt tgtcacacaa atcgttgaat caggatttaa cggtatcttt
      481 ttagttgccg ctaacccagt agatattttg acttactcaa cttggaaatt ctcaggtttc
      541 cctaaagaac gcgtaatcgg ttcaggtacc tctcttgact cagctcgttt ccgtcaagca
      601 cttgcggaat tagttgacgt tgatgcacgt aacgtccatg cttacatctt aggtgaacac
      661 ggagattcag aattcccagt ttggtcacat gcaaatgttg ccggcttgca aatctatgaa
      721 tgggtaaaaa acaacccaga aatcgatgaa gaagctatgg ttaacctatt ctttggcgta
      781 cgtgacgctg cttacacaat tatcgagaaa aaaggcgcta ctttctatgg tatcgcagta
      841 gcattagcac gtatcactcg tgccatctta gatgatgaaa atgctgttct tcctttatca
      901 gtattcatga atggcgaata tggattgaac gacatctaca ttggtgcacc tgctgttatc
      961 aaccgtcaag gtatccaaaa agtaatcgaa attcctttaa gtgacagcga gcaagaccgt
     1021 atggctgctt ctgctaagca attaaaagaa attctagatg aagcttttgc taaactagac
     1081 gctgaataat ctttgaacat gacggaaaac atctattcgg tgttttccgt ttttttattt
     1141 tatcaccctt atggtttcta aattttttca taaagtagag ataaccttcg tcttttcctc
     1201 taaattatgc tatcatgaga ccgttccacc ctaaactttt acttaaggag ttgtcgatat
     1261 gacaagatct tcgcatcgaa aaaaatcgaa acatgttgct ttgctttcag ttcttggtgt
     1321 tctcttagtg ctgatcagct tttataccta tcgcagtaca tactacacaa agcatttttt
     1381 gccgaataca gaaataaatg gaataaatgt cagcaatcta acagtcgaaa aagcaaatga
     1441 aaaattaaaa gaagcttatt ctgataaaaa actggctatt aaagaagatg gaaaagtctg
     1501 gcaggaagta ccaaaatccg agctcggcta caaagatgat ttcacttcgg agctttctcc
     1561 gatcctaaat gaacaaaacg gatggacttg gggaatgact tatgtctcag cggcagaaaa
     1621 acaagaaata gatcctgtca gtcaaaacca ccaaaaacta gacactaccg ttcagacatt
     1681 gacaactaag ctgactgatt tgaacaaaga ccggacacct actactgatg caacgatcga
     1741 aaaagcaggt gactctttcg ttatcaagcc tgaagtcaat ggaaatacga tcgacgtcga
     1801 tgctgcggtc aaacaattaa aaagtgcggt aaactctggt aaagatacaa ttgaattgac
     1861 tgaattcaaa gaaaaaccaa aagtaacatc tgaagacagc tcactgaaag aacaactcgc
     1921 atccatgaat gctatcgcaa atgtaaaagc tacttacagt ataaacggtg agactttcca
     1981 gatcccatca agtgatatta tgagttggct gacctacaat gatggaaaag tcgatttaga
     2041 tacagaacaa gtacgtcaat atgtaacaga tttaggcaca aagtataaca caagtacgaa
     2101 tgacacaaaa tttaaaagta caaaaagagg agaagttacg gtcccagttg gcacttacag
     2161 ttggacgatc caaacagaca gtgagacaga agcattgaaa aaagcgattc tggcaggcca
     2221 agattttact cgttcaccca ttgtgcaagg tggcacaacc gctgatcatc cattgatcga
     2281 agatacgtat atcgaagttg atctggagaa ccagcatatg tggtattaca aagacggaaa
     2341 agtggccctt gaaacagata tcgtttccgg aaagccaact acacctacac ctgctggagt
     2401 cttctatgtt tggaataaag aagaagatgc aactttaaaa ggaacaaatg acgatggaac
     2461 accttacgag agtccggtaa actactggat gccgattgac tggacaggtg ttggtatcca
     2521 cgattcagac tggcaacctg aatacggtgg agacttatgg aaaacccgcg gttcacacgg
     2581 ctgcatcaac accccaccaa gtgtaatgaa ggaattattc gggatggtcg aaaaaggaac
     2641 gcctgtattg gtcttctaac aaaaataata tataaaaaga aagcacccgt acgattggta
     2701 caggtgcttt ctttttatta tctaacaatg tgctacttac atttcagcag cagtaaagac
     2761 ttctgaaaca tcatcatcgt cttctagctt atctactaga cgttccaatt gctctttttg
     2821 ttcctcattt aaatcaatcg tcgtttgtgg aatcatcgtc aattctgctt gcgccaaagt
     2881 gaactctttt tccaactcat ctcgtacagc agcaaaatct tctggtgcag tataaatctc
     2941 gaatacttca ggagaagttt gcagatcttc tgcccccgcc tctaatacat cctcaaacat
     3001 cgtctcttca tcaacagtca acccatcgcg ttcaatagcg atatatcctt tgcggtcaaa
     3061 caagtaactg actgaacccg tttctcccag tgatccaccg tttcgagtaa aagctactcg
     3121 gacatttgtc gctgtccgat tacggttatc tgtcaatgca tggactaaga ttcctactcc
     3181 acccggacca tatccttcat acgtgatttc atcgtaattc gtttcatcta cggcactcgt
     3241 agccttttta atcgcccgtt ccacattgtc gttaggcata tttgcagctt ttgctttgtc
     3301 cacaacaagc cgcaaagctg gattcataga tggatcagga ccgcctgctt tagctgccat
     3361 ataaatttca cgagaaagtt tctgaaagac tttccctcgt tttgcatctt gggcattttt
     3421 tcttccttgg atattatgcc atttactatg tcctgacatg atttttcctc ccttgttctt
     3481 ttgagaatac acttctattc tagtactatt tcacaaaaaa acaaagccac agccaatcta
     3541 taaacgcttc ttgaatgccg caaaaagcac acataatatc acagcagtca gagcaccgat
     3601 cgaatccaac atgacatctt ggaacatagg tgttcgtcca ccggtcatca tctgatggaa
     3661 ttcgtccaaa cctgcgtacc ctgttgctga aagccaagca aggatcgctg tcagccacca
     3721 aatttttagc ttggggaaaa cacctaaaaa caagctgcct cccaaaataa aataggtacc
     3781 gaaatgtgcg cccttcctta aaaagaactc taaaaatttg gcgtagccca gatggttgat
     3841 actcaccata cttccaccat aattaaaacg tatacccgct aatgtgtctt taaatggctg
     3901 attaggcagc cagctttcta atcgcgaaac ttgtgattgc tgtccgtaag tttgagaaga
     3961 actgtaaaat aagattataa cgactatcag tgcgattact aaataaatat ttccgttttt
     4021 catttgtttt tttgaagaat tcataagtca cctctgtatt ttatcttaga ctttttttga
     4081 agaaacaaca atcattttca tagagacttc ttccataaaa aagtgctata cttaattttg
     4141 tattacaata tgaagggagt tttttcatga gttattcatt aaattggaat ctagattcta
     4201 ttttttccgg aggcagtcat tctgatgctt tgaaccaacg gatgaagcaa ttggaagacc
     4261 aaatgaacga gtattatcac cgcgtaacaa aatggtcacc tagttctgac aatgcagagc
     4321 agttaaacgc tattcttcaa ttgcaagaaa cgattacgaa tggttttacc caatgcagca
     4381 gctatattac tgcattgctt tctgccaatg tcaatgattc agatgcaaag gtcttatcag
     4441 gcaagcttta cgcaatgctg cctcgtctgc aatcagccga aactgtattg tcaaagaaat
     4501 ttgcagaaat atctgacaat gattggaatc agatgctttc tcaatcttcg tttgaaacaa
     4561 ttgcttttcg tttaaacgag attcgtcgtg atggctcaca attattatca gaagctgaag
     4621 aaaacattat aaatacgctt tctttagatg gtctaaatgc ttggagcagt cattatgata
     4681 cgattgtagc cagcatttcg attcccttcg aacaagatgg acaagtagtc gaattatctg
     4741 ctggacaagc cttcaataag atgatgggcg atccagatcc aaaagtcaga gaaacacttt
     4801 tcgcagcatg ggaagaagcc tggaaagaaa aggcacccct cttcacggat actttgaatc
     4861 atttagatgg ttttagactt tctgactatg aacttcatgg tgtcacagat tttctacaaa
     4921 aaccattaga gtacaatcgg ttgaagaaag aaacattgtc tgtcatgtgg gatacaatcc
     4981 agaaaaacaa acaacccctt gttaattact tgactagaaa agctaatctt ttcggtaaag
     5041 aaaaaatgga atggcaagat caagatgcac ctattatttt aggcgatttg aaagaaaaaa
     5101 cctttagttt tgacgaagca gcagcattta ttatcgaaaa ctttaacaaa ttcagtccaa
     5161 aaatggctaa atttgcccaa tctgcttttg aaaagagctg gatcgaagca gaagatcgtc
     5221 ctgggaaacg tcctggtggt tattgtactg agttaccaga aacaaaagaa tcaaggatct
     5281 ttatgaccta cagtaattct gttaatgagg ttgcgacact tgctcatgaa ctaggacatg
     5341 ctttccatag cagcacgatg tgggatttac cttctttaaa tcgcgaatat gccatgaatg
     5401 tcgcagaaac agcaagtaca ttcgcagaac tgatcgtagc cgatgctaca ttaaaagtcg
     5461 caaaaacaaa agaagaaaaa atcaatttgc tagatacaaa actgcaaaat gctttagcta
     5521 tgttcatgaa tatccattcc cgattcattt ttgaaaatcg attctatgaa gcacggcaag
     5581 aaggattagt ctcagaagat aagatcacag agatgatggt tgaggcgcaa aaagaaggct
     5641 atcaaggagc tttagctact taccatccat atttctgggc tgcaaaattg catttcttta
     5701 ttgatgacgt accgttttat aacttcccat acacatttgg ctacctcttt agtttgggca
     5761 tctatgctta cgccaaccaa aagggagcaa attttgaaga agaatatatc catttactac
     5821 gcgatactgc ctcaatgacg acggaagatc ttgcgaagaa acatttgaat gtcgatctta
     5881 cccaaccaga tttttggcaa gcaggtatcg acatggtgac tgaagatatc caaacgttca
     5941 tggaattgac agaagaattt atctgatttc tattttatca caataaaaaa agagttagtc
     6001 cagaatgggc taactctttt tgattaatca ttcttacatg ctgacagcaa agtcaggggc
     6061 aaaccattga actgaaccaa cttttacgct ttcgcctggt tgaggagcat ggatatattg
     6121 tcctccgcct aaagcaatcg ctacgtggta agttccgcct tgtgaccccc agaataacaa
     6181 gtctccagct tttgcttgag aaactgaaat ttttgttcca gctgattctt gaggaactgt
     6241 ccaaccaccg atgtcacgac cagttacttg catgtaaaca tagcgtgtga accctgagca
     6301 gtcaaagcca cttggatctt taccgcccca aacatatgga gtaccaatat atttgtaagc
     6361 ttctgctacg attgctgcac cattcacact tccagaagga gctggagcag gtgttacagg
     6421 ttttggttgt tcaggtgttg gtgttggcgt tggagccgga gtactacttc ctgtgccgtt
     6481 tcctgtatca acactttgat ctgttgatgg tgttgtaggc gcaggtgttg ttgattcttc
     6541 tgttgttgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcactgt
     6601 tgttgattct tctgttgctg aagattcagg cgctgttgtt gattcttctg ttgctgaaga
     6661 ttcaggcgct gttgattctt ctgttgaaga ttcaggtgtt gttgattctt ctgttgctga
     6721 agattgtgta gctgatgatg attcttcagt cattgaagat tgtgtagctg atgattcttc
     6781 tgttgctgag ctttgtgcag tagatgaagc ttgagtagct tgtgcttcag cctctgcttg
     6841 ttcgcgtgct tctttttcag ctttttcttg tgcagcttgt tgacgagctt gttctgctag
     6901 acgttgttgt tcacgaatac gagcttgctc tgcttcagct tctgcttttt gacggttcaa
     6961 gtcagctttc ttgtcttcag cagttgcttg ttcagcagct aatgaagttt tcagtacgtt
     7021 caaatcagct tgtttagata aaagatcgcc tttttgagat tccaaagcag cttggttttc
     7081 agctaattct tttagtttag catcgttttc agcttttttg tcttctacag cttttttatc
     7141 ttgtttttgt tgttccatca agtcgttgtt tgcttttacc atcgttgtca ttgcttgaac
     7201 gcgtccaata gcatctgcta atgaatcagc attcaataca gcatcgatgt agtttgagct
     7261 tgtgttgctt acttgtgctt cgcgtgcttg tttttggatt gtatcttcac gtttttcaat
     7321 acgttcttgt aaatctgcaa tgtcttttac taattgtgca gattcttgac gcaatgtatc
     7381 ttgttttgct aataggtctt gtgcttgtgt gttgatttca gaaacttggc tttctaaggc
     7441 atcgatttgt gattgtgcat ctgcttgttg atttttcaag tctgcaatct tttgatcttg
     7501 ctgttgtatc tgagaatcaa aatcgtccgc cgcggcagcg atcggtgatg ctacggcagt
     7561 caaggtcatt gaacatacca ttactgctga tattaaactc tttttcactc ttcattcctc
     7621 cgactggctt aatttaataa atatctaaat atatttaagt ctttcttaac gatataaaaa
     7681 ttgtaccata gcagtgtgtc aacgatataa tagttatgtt acaaaaatgt ttcaaaacaa
     7741 cttttaaaaa tgacaaaact gttcgtttca gaaaatgatc atgaaaaagc cgcgttaagt
     7801 ctagcatttc ttaagataac aacgattctt tttcgctttc gacctaaaaa taaaaaaaga
     7861 aattattcat cactgaataa tttcttaaag ggaaaaatga ataatacaaa tataatcatg
     7921 ttcaacagca gtgtaggtcc taaaaacctt gtaataaaat acgtgttatt tactactgct
     7981 aatttaaaaa tcaactggac caccatcgta aacaattcat aacccgtgac aaagatgatc
     8041 atactgaaaa attcagtaaa gatgttgaca tggataacgt ctttcattac atacatcaac
     8101 catacaatta aagggagagc tactgcatag ataccgataa cgccaatata gtaagcgtca
     8161 tagatagccc cgagaacaat cgtggtaatc agtaaataac gtttttcaaa cgccatactg
     8221 caacataata atgccaatat cagaaagtgg gcatttgaca tataggtgcc tttcgaccac
     8281 tcccctagca ttcttgttaa atgaccatct atcaacatca aaaagaacaa aacgattggc
     8341 agatagtact tcattgtttc tttcttcaac atggactaat cctccgctag ccgttggaca
     8401 atcgtcacaa ctgacacgtc atacatttct gcataaggtt tcacataaac ttcccgatcc
     8461 agcccataac tatctggttt cgttttaata accgtaccga tcggcaagtt agctggagaa
     8521 ttccctccaa gaccggatgt ctggacaaca tccccttctt tgatatccgt atcaccagtt
     8581 aattgggtca ccactaaggc atggagcttt tcatcatagt ttttaagcaa accaaacgct
     8641 tcgccattag ccgaagatac ccgtactgga aaatgattgg agctttcatt agaggatgac
     8701 agtaattcga ttttagacga agccgtattg acctcgatca ctcggccaat caatcctttt
     8761 tgcgacatga ctgccatatt tgcttcgata ccgtctttag aacccttgtc tacgatcagc
     8821 atgtcctgcc aactgtcagg cgaacgagta atgacatttg ccgttacttt ttcgtaacta
     8881 gtcagcgtct gattcaactc aagttctttt ttcaacgctt cgatttctct ctcgtaattc
     8941 ttgttttgct gtgaaacgtc atcatagcta tcgatttttt cttttagccg ctcattttcg
     9001 gcataagttg aaaaaagatt atggacagaa tctactccat ttcgtaccca ccgtactggg
     9061 gcagagattg ttttatcaac cattgcaaca ctgtcattaa cgatggattg gaagaaattg
     9121 ggctttcctt cattcgctcg ctgcgcagcg gtcacactta gtatcgtcac cacgatgata
     9181 acaataacca aggtaataat aatattttta ttaggattga acttcttcac aaagacacct
     9241 cgaattaatt agactcttgt gttcaatttt atcactaaat gccgtgaaac aaaaggtaag
     9301 ttgctcctct taagcttttt tttagcaaat aatagattct gtcattttta gcctgaaaga
     9361 caaatttttt tatttgttta tggatcaatc tttgctttga ataactgcaa tcataccatc
     9421 tgaaaaagaa aattctgcgg tatctgaaag aaattcgtta cttgcataag atgtcggata
     9481 ttctacttct ttattttcca atacatactt gctgcctttt aatgtaagat tcgagacggg
     9541 agtcaagcag caataagcta aatacttcat atctggtatc tgtcttacta tatgctctcc
     9601 aggcaaataa taagacaaat aattttgaca atctctgata ataagctgat gggcaaaggg
     9661 tttgaagcga ggttccatac ctaaccagag attggctaaa agatgatcca atcttccgcc
     9721 cgtagctcca atcaaagtga cttccgcttg cgggtaacgt tcaaatgttt ttaagatacc
     9781 taactgtgta tctgtatcgt ctttttctgc aggggaacgg gtatactctt tggcttttgc
     9841 aaatacttcc ttctgctctt cgttcgacaa tgaatcaaaa tcacccactg ccagatctac
     9901 tgggtagcct gcttgaagca ggtacagcgc accacggtct atcccgacat aggcatcgta
     9961 tttggacagc agagactgcg gccagttttg cggattacct ccagcagcta ataaaatttt
    10021 catttagtct aatgcctctt ttaatcgttc gatttgtccg actggatttt cggctccata
    10081 gatatatgat cctgcaacga agacatccgc ccctgcatct ttacagattt ttgctgtttc
    10141 tggaacgatt ccgccatcta cttcgattgc ataagtccaa ttgttctctc tacgtagatc
    10201 agcgagttcc gtaatttttt ctactgtttc ttcaataaag ctttgtccgc cgaacccagg
    10261 attgacagtc atgactagta cttgatcagc caaaggcaaa acatgtttga tcatggagac
    10321 tggtgttcct ggattgataa ctactccagc tttaactcct aagctttttg tcatttgaag
    10381 tgcccgatga atatgaggag ttgcttcgac gtggacagta atgatatctg ctccagcttt
    10441 tgcaaatgct ggaatgtagt tttctggatc aacgatcatc agatggacgt ctaacggtaa
    10501 tttagtaaca ggacggattg cctgaacaat atttggccct aatgtgatat ttgggacaaa
    10561 tcggccatcc attacatcta cgtggatgta atctgcgcct cctttttcca ctaactcaat
    10621 gtctctttgt aaattggcaa aatctgcact caaaatggat ggtgctatct tcattcttgt
    10681 ttcctcctat tttcgtttac cttctttttt tgcatacatt ggttttcttt tttcgatttc
    10741 ttgtaagaat tgcaaataat tgtcgtaacg tgtctgtgcg ataccacctg tttctactct
    10801 atgttttact tcgcagccag gttctttagc atgcatgcac tctctaaatt tgcaatggac
    10861 agaagcttct acaaattcag gaaattgtct cggcagttct cctgcttcaa ttgtcaaaaa
    10921 gtcaatcgag ctaaatcctg gtgtatctgc tactaacccg ccatagatag gaatcagttc
    10981 cacatgacgt gttgtatgcc gtcctcttcc aagtgcatca gaaatctctc ctgttgctaa
    11041 attcagttcc ggtgatattt gattcagtag cgtcgatttt cctgctccag actgacccat
    11101 aaagaccgat agccgttctg ggaagtaacg gatcagttct gtcgtatcac ccgtatgatt
    11161 gggtacaatc accggatagc ctattttagt ataggtttgc ttgatctctt cgattttttc
    11221 tgggtcgtct gacaagtcgg tctttgtcaa aaaaataatc ggttcgatcg actcgtattc
    11281 caaggtaact agaaagcggt caagtagatt ataagaaaat gcaggctcta cgatacttgt
    11341 gacaatgatt ccaagatcaa catttgtgac tggtgggcga actaattgat tttttctcgg
    11401 caagatttcc agaagatagc cgtctgtttg attttcactc tcaaagatca cctgatctcc
    11461 gacaagcggc gtgattttcc gattccggaa atttcctctt cctcttgttt gataggtctc
    11521 tccgtttgcg tatacatagt aaaaaccgct gattgctttt ctgatttgtc ctttgataag
    11581 tgccacctcc gtttttgttt attttaccat acgaagagga aagtaggtgt tattccatgc
    11641 aaacttctca aagtatatgg cttgaaaatt gatcaaaaga gatagaaaca caaaaaagag
    11701 aatgcaacaa atcaatgtcg cattcccttc tgatatattt tattcagcac ttattttgaa
    11761 ctttctgagg tgctagctga tgaacttgtg gagctggaag attcttcttt tgaagagttg
    11821 cttgtgctac tggagctact tgagctgcta gaactgtctg aagagtcttt ttcagaacta
    11881 gaagacgctg gatctggtcc tttagagtaa attacgttca caaccattcc tttggtaatt
    11941 gtcgttcctg cccctggtac tgtacgaatc actttatctt tatccaccgt gtttgaatat
    12001 tcggaagtct catgcccgat atattctgca ccgacactag ctaaataact ttgtgcttcg
    12061 ctttttgtat agccggagat atcattcaat ttaggctctg ttccttggct tacgacaaaa
    12121 ttgatgtaag tttcttctgc tacaacttca cccgaggctg gatcctgact gatgatcgaa
    12181 cctgatggta cagtgtcgct atagtcataa gtagcactaa tattattgat attgaatcct
    12241 tgtgtaacaa gccaattacg aacagtttcg tagttttctc ctacatgact actcaaagtc
    12301 actgtttctg gtcctttgct gactgtgaaa gtaatcgtgt ctgttttagg atcgacttct
    12361 gtatcttctg atggagattg cgaaatgatc attccgcttt ctacctctga actgaattcc
    12421 tcttcttttt gaatcaagtc agcagaaaac cctaattctt tcaaacttgc ttcggcatct
    12481 tcataacttt taccactgta atcatctaat ttgatttttt tcgtgccgct actaatatac
    12541 agagtgactt cgcgtttttg cttgactgtc gttcccgctg ccgggtctgt cttcactaca
    12601 ttaccttctt cgattttgtc atctgcaatt tcttctgttt ttgttgctac ttctaatttc
    12661 gcttttgcga gtgtttctct agcagaagct tcagaaagat cagaaacatc aggaacaaca
    12721 acttctcctc gcccgccaga agcaaagaat acagctgttc cgattcctaa aacagcaaga
    12781 atagctaata ggatcagcca ccattttttc tgtcctttct ttttaggatc aggcagaact
    12841 tcttcctcct ctggtaaggt ttcgttttct ggcttgtctt ttggcagagg catagatttg
    12901 aatgaatcag gcattggtgc atcttccgta aggggtgtga taacttttgt ttcattatcc
    12961 atcacatgag gctgccattt ttcttcgttt gcacgagctg gtgataatac tgtagaaaga
    13021 tcatcagaca tttcctcggc tgacttataa cggtctgcag gttctttagc cgttgcccgt
    13081 aaaatcacgt tttccagtgc ttgtggcaca tttggatcaa gtgcttttat agatggaaga
    13141 tcatcttgaa aatgttttaa agcaattgtt acagcagatt caccatcaaa cggtacgcta
    13201 cccgtcaaca tctcataaag gatgatccct aaagcataaa tatctgactg cttcgttgcc
    13261 atacttcctc tggcctgctc aggggacaga taatgaacag atcctagcat cgtattcgtc
    13321 tgcgtgatcg atgtttcaga taaagcgata gcaatcccga aatccgtgat ttttacaaca
    13381 ccttcattat cgatcagtac gttttggggt ttcaaatctc ggtggatgat ttgatggtta
    13441 tgagctaaag aaatagcaga aaggatctgc tgcataatat ttactgctgt ttcataagga
    13501 acaggataat gtgtttggat atatcgtttc aagtccattc ctttgacgta ttccatcacc
    13561 agatactgca tgttgtcttc ttcaccaaca tcgtaaacac taacaatgtt tggatgaacg
    13621 agttctgttg ctgctaaagc ttctctctga aatcgtcgaa tcgcagtctg gtcattttga
    13681 aagtcaaatc gtaagacttt aactgctaca tcacgatcca agattaaatc gtgcgccaaa
    13741 aatacatttg ccataccacc gctaccgata tttccagtga tttgataacg gccatttaat
    13801 tttttgccca actcgatcat acactgtcct ccttatattc gataagaaga acggtgatat
    13861 tatccgctcc gcctgcttca ttggcacgat caatcagctc agttacttta tgagacaatg
    13921 ggccttcttg attgatgatc gttcctattt cttcttcaga cagcatgttc gtcaagccat
    13981 ctgaacataa cagcagtcga tcttttagtt gccaaagatg agtagaaaca tctacttcga
    14041 ctgttccagg catgccgaca gaacgtgtta aaatattttt ccttggatgg ttcactgcca
    14101 tttcttcgct gatttctcct gatttgacta gttcattgac taaagaatga tcctccgtca
    14161 attgcatgat tttatcttca cgaatcaaat aagctctgct atccccaaca ttggcaatcg
    14221 tgaaccagtc atctgacaga gcggccgcta cgattgttgt tcccataccg ttatattccg
    14281 gctgttcttg tccacgctga taaatcagca tattttcttc ttgaatcgat tgaatgaacc
    14341 attgggcgat tttttcatcg tcaacagctt cttgtttttc ccaagcttcg cctagattcg
    14401 ttaccgccat ctgactggca atgtctccgg cacgatgacc acccatcccg tctgctaata
    14461 cagcaagctt gagacctgaa cggctggtaa atacactggc atagtcttga tttgtgttac
    14521 gtcgtcttcc tacatctgat tgatattcaa tctgcatttt ttcacctcat tactttttgc
    14581 gcaaacaaca aatgaaaaat ccatctgtgt aaaactgatg cggatacaaa gtcaacattt
    14641 tctcttcgat tgatgattgt agcataggat tggtcaaaac atcaatcctc tcaaaatcag
    14701 ggtgtctttc caagaaagca gaaacaacgt cttggttttc ttcttttaag atcgtacaag
    14761 tgctataagt cagaataccg gaagacttta aagttggtgc gcagctttct aaaatttcca
    14821 attggatttt tggtaaattc gctaattcat ccggctgttt tttataacga atatctggtt
    14881 ttcgccgaag cagcccaaga cctgaacaag gcgcgtctac taaaatccga tcaaactgct
    14941 ctgcttcgaa ctgtttactg acttctctag catccatctt ttgagcgtag acagcagaag
    15001 agacacctag acgtgctgca ttatcttcaa tgagtttgac tttgtgttcg tgtacatcta
    15061 aagcgaccac ttctccaccg catgacggat ctaaaaaaga agcaatatgc gtcgtcttac
    15121 ctccgggtgc tgcacaggca tctaagacat gatgttcagg ctcgatttgc atagacggcg
    15181 ctacaagcat cgaactttca tcttggatcg tcatgacgcc atcgtggaac aatgagctag
    15241 aagctaaatg cccttttttc gctactatac cgtatggact gacttggctt ttttcagctg
    15301 tgatctcttc ttcttgcaaa acagtgatgg cctgatctct ggaaagtcgg cgtgtatcta
    15361 ctcgggcact agcatggcta ggctgatata gcgataaacc gagcttttca gtttcttcgt
    15421 atccgatttg atccaccagt cgtttcgtca tccatagagg caggctgatt ttgacagaaa
    15481 gtctttcgat aggatcagca atgccttgca gatctggtgc tccttgacgc tgataattcc
    15541 tcaagacacc atttacaaat ttccctgtcc caggatttcc ttttactttt gcgatttcga
    15601 ccgcctcgtt taaaatagca tgtgcaggga ctttatctaa aaattctaat tggtatacag
    15661 aaagataaag aagcatcttg acccagtcat ccactttttt tgctttagcg atcaatggtg
    15721 caacgtaaaa ttccaatagt cgctgatggc taatcgtacc atagatcaat tctgtaaata
    15781 aacccacatc tttttgattt agctcgcttt gtttgatcat ctcattcaac aaaagattcg
    15841 agtaggcgcc acccttgttc actcgctcta atgtcagtaa tgccatatat cgtactgatt
    15901 ttttcatttt tttagggatt tttttcatta tttcacctgc tgaccaatct gaacttgctg
    15961 accgcttccg ttaaggaaat catgtacggc ctgttttcct ttaccagctg gctgtaattc
    16021 attgatggct aagacggtct gtttaccgca agcaatccat agattttttt tgtctttccg
    16081 gatgatcgta ccaggtgctt tgtctgtagt ttctgccaat acttcaaccg ataataattt
    16141 ccagcgtatg ccctcatagg tcgtgaatgc aattggccaa ggacgcattc cgcgtacttg
    16201 gttgtcaatt tcttctgcag ttttatgcca atcgattgct tcttgttccc gtgtgatgtt
    16261 tggcgaaaaa gttgcttctg actcgtcttg aggtactggt ttttgtccat ccaaaatatt
    16321 gggcaatgtt tctagcaata gctttcttcc taaaaggcta agcttttcaa acatcgtccc
    16381 tacatcatct tgtttagtga ttggtatgct ttcttgcgca tagattccgc ctgcatccat
    16441 ttttttgatc atttccatga tcgttacacc agtttctttc tccccattca tgatggcata
    16501 atgaacaggc gcacctcccc gatatttagg aagtaaagaa gcatggacat tgattgcccc
    16561 taattttggt acttgcaata acttttcagg caagaattgg ccaaatgccg cagtgacgat
    16621 cacatcaggc accaattcaa tgatttcttc catttctggc gaaccggaga ttttctctgg
    16681 ctgcagcaca cggataccgt gcttcagagc cgcttttttt accggtgtcg gcgtaattac
    16741 tcgtttccgc ccaactggac ggtcgggctg tgttaccact gcctggatct cgtaacctga
    16801 ttctatcagc ccttcaagaa tcggcacaga aaaagcaggt gtgcccataa atactagctt
    16861 agtcatcaag atgttcctcc atatattctt caagttcttc ttccgggatc tgctgtatca
    16921 ttttctcaat gaacaagata ccgtctaagt gatcgatttc atgctggaag gcacgggcta
    16981 aatagccaaa agccgtcact tctatctctt ctccgtctcg atcataataa cgcacggtca
    17041 cttcatctgc tcgcttgacc gttccataga catgcggaat actgagacaa ccttctacgt
    17101 caaggctttc accttttgct tcgatgattt ctgggttaat cagttcaaat ttctcgcctt
    17161 catctacttc gatcacagca attctcttgt tttgtcctac ttgaggagca gcgatcccta
    17221 ttccgtcatt cgcaatcatt gtctcatata aattgtctaa taaagcgatt gtctcgtccg
    17281 tgattacatc gattggctga gcagttcttt ttaatttatc attagggtgg atcaatatag
    17341 gatagcgcat aatttggccc tttcttaaat aaagtttaat ggttcgttgt caattgccac
    17401 aaaaagacct tgtcgctgtt ctttctggct tccattcaaa atttcatcca aaacatgagg
    17461 aagattcggt tcatgtttat atttgatgat tagttgataa taatatcggt ttttcactct
    17521 caaaatcatg ctgggcgttg gccctaatag caagctttga gggcttaaca cttcttttag
    17581 ctgattggca atctgaaaga ttttctttgc cgctacttgt tcttcaggat ggctggcggt
    17641 tattttcaca gtaaagtaat aaggaggata gcctccacga tgccgtacat gcatttcctg
    17701 ctgataaaac tgctcataat tttgtttttt agccaattct atggcgtaat gttgaggatt
    17761 gaatgtttgg atcacgactt cgcctgcttt ttccgctcgc cctgctcgtc ctgaaacttg
    17821 ggtaagcaat tggaatgtat gctcactaga acgaaaatcc ggtaaattca gggaggtatc
    17881 agcattcaac accccaacta atgtcacttc tggaaagtcc aaacctttag caatcatttg
    17941 tgtccctaat aatatatctg cttcttttgc accgaatttt tgtaaaatct gttcgtgtgc
    18001 cccttttctt cgagtggtat ctacatccat acgcaaaatc ctagcttgcg gataaagttc
    18061 tcggagttcc tcttctactt tttgagtacc tgttccatag tagcggattt tattgccgcc
    18121 acaattagga cagcgattgg gaataggttc ttcatgtccg caataatggc agcgcattga
    18181 acgtgtatcc atatgcagtg taagagagat atcgcaatta ggacatggaa gaacaaatcc
    18241 acagtcccta cacatcacaa aagacgaata tcctcgacga ttgagcagta ggacagtttg
    18301 ttcttttttg tctaatcgat tttgtatttt ttcctgcaaa ttagcagaaa aagtagaggt
    18361 ccgatgattc tgaaactctt cacgcatatc tacgatctca atagctggaa gctgtgcagc
    18421 tgctttggct cggtggttta actgcaataa ctgataaacg cctttttgag ctcgtgctct
    18481 tgattctaaa gagggcgtcg cgcttcctag aactattggg caatgatgat atttactgcg
    18541 ccaaattgct agatctctcg catgataacg cggtgtctca tcttgtttgt aacttgattc
    18601 atgttcttca tcgataatga tcagcccgat atttttcagc ggggcaaaaa tagcagatcg
    18661 tgcgcctact actacttgcg cttcttcccg ctcaatcttg cgccattcat catatttttc
    18721 accttgggaa agtccgctat gtaagaccgc aactgattct ccgaagcgtc ctttgaatcg
    18781 ctcgaccatt tgcggagtca atgcgatttc aggcactaac ataatagccg ttttattttc
    18841 tgacaaaaca tcagcgattg cctgaagata tacttctgtt ttcccgctgc cagtgattcc
    18901 ctccagcaaa aagacctgat ctttttgctg ctgacctgct gtgatgattt gttcgacagc
    18961 atttttttgt tctgcattca gttccaatgc cgtcgtttgg tcaaatactc ggtctttata
    19021 agggtcgcga taacgttctg attctataaa ctccagccag cccttttctt ttccttgatt
    19081 caagacagca gtactgattc ctttattttt gaaataagca attggttctt cttggctcag
    19141 ttcagataaa cacaaaagaa gttctttttg tttcttggca ttttgccgta attttgccca
    19201 ttcttcttcg atttgctttt cagttaacaa agaacggacc aaacgaatcg tttttacttt
    19261 atttcgtgta tggacttcat atttgatatc tactaattgc tcttggcgcc agcgaatcaa
    19321 ttgagaaagg ctgccttctt cttctgcttc cttccaggaa atcacttctc gctgatcaaa
    19381 caatacctga acttctggtg tgtctgccaa aggatagatc aatttatcat attctgcttt
    19441 catcacactc ggaagcatcg tttgtagaca agtgattttg aatgcgtaag taattttttt
    19501 catataatca gctaatgaaa gtaattcttc attgaccacc ggagcaaggt ctagcaaacg
    19561 aataactgcc ttcaaagatg gattagctga ttcttctgat ttttggatag ccataacaaa
    19621 tccttggaca tgacgattgc cattaccaaa agggacttct actcgcatcc cgacttgcaa
    19681 agctgtttcc atttcagagg gaaccaaata agtgaatggc tgatcggtct gcatagtagg
    19741 tacgtccaca atgacttcag ctgttaatgt catggttcgt ttcctccttt ttttgtcttt
    19801 tcttatcaag cgacaaaaag ttccttgcct attttactag agatcgatgg atttgtctta
    19861 tcttctgcca aatcctaaca aaaaattaac agcagataag aaaaaaaaga actgtgtcaa
    19921 aaagttcaga cacagccttt tctaccagac aataagtggc gaatgtttga aaaggaaaca
    19981 gctgggtttg cttttctttt tagatgttcg ctctattttt ctttattaca ttttttgctc
    20041 ttcacggata cgtgattcca attcgtgttg ttcacgctct ttttgtgctt gacgttcctc
    20101 ttcttccatc ttcaaacgtt cacgttttaa ttcaggatgt ggatcattga ctacatttcc
    20161 tgcatcgatt tcttctaaag cttgaccgac gctttttact gaatcaaaag aatctagagt
    20221 aggctgtgca cttgcatcca attcatgcgc gcgtttactt gctaaaatca ctagagaata
    20281 ttttgaattt acacgatcta atagtgagtc aattgaaggt tttaacatca taagactaca
    20341 tctcctttaa cattttaata tatttaccga ttacgcgatc tactcgaaaa tgttcactgg
    20401 caataatgtt tttgattcgt tcaactgcta gcggcacttc atcatttaca acagcatagt
    20461 cgtaaagagc catcatttcg atttcttctt ttgcaacttt cattcgtttt tcgattactt
    20521 catctgcatc tgttcctcgg cctacgatcc gtgattttaa ttccgctaaa tctggcggag
    20581 tcaaaaagat gaatacacca tcgggcactt tttctttgac ttgttttgct ccttggactt
    20641 caatctccaa gaaaacatct tttccttcat ccaatgtctt gttgacatat gacaaaggag
    20701 ttccataata attcccgaca tattctgcgt attccagcat ctctccagct tcgatcattg
    20761 attcaaactc ttctttgctt cggaagaaat aatcaacgcc ttcgacctcc ccttcacgca
    20821 ttttacgggt agtcattgag atcgaatact gaaaatcatt atcctcactc tcaaaaattg
    20881 cttttcgtac tgtgcctttt cctacaccgg atggaccaga caacacgatt aataatccac
    20941 gctctgacat gacgacgtcc tttcaactag tctttttgta cttatcgttt attttgcact
    21001 ttttttctgc aactttcaag gttatcttta attatagcta gaaattggta aaaaagctac
    21061 cctgtaccaa gaggtcgtaa aaaagctgtc ctgcatcaag taataagaac agccaaacat
    21121 aaacagcttc tcatgttttt cgtttggttg gggttattat cgcagatgca agcttttgaa
    21181 cgccgtgtat aagaggtcgt gaagaagccg attagtttcg agtatgaaga aaaattttgt
    21241 gaaaatagtt tctcacattt ttgcataaat ttggcttaat accgagaaaa tggtttttga
    21301 acatcactta ttcaaaataa aaaaagattg tgacacgaat tttgtcacaa cctttaaatg
    21361 tattcctaga gcaaagaagc taagcgaaga aatccaattc tgtttattta ttgtatttct
    21421 ttgttcagca tccttgctaa aagcccatat tggacaaagt attcactaca cccatgatct
    21481 catcttttcg agaacgtgct ttttctgtcg gatcttctac ctcttctgtg tcaattcgaa
    21541 caataaattt ccgattcatc gtcacgtaag caccgtcgtt gatcaaaata aacagttcgt
    21601 tctcattata gtctgcacag gcatctttcc atacgtcaaa gccgtttttt tcagaaggga
    21661 tatcccgttt aaccacagtt tttccactta attcgtggaa cgttacgtca taatatttca
    21721 ttatctctac ctctttcgtc attcaatatt ttgtacttgt tctctgattt tttctaagat
    21781 cgtcttagac tggacgactt gatttttaat ttcgattgca tttgatttgg aaccgatcgt
    21841 gttgatttcc cgattcattt cttggatcag gaaatctaat tcccgaccta caggacattg
    21901 tgcttcgata agttcatcta gtttttgtac atggatcgcc agacgatcca gttcctcgtg
    21961 aatatctccg cgttccaaca agatggctag ctccgtcaac aaccgttgct catcgacttt
    22021 cgctccaaga aattcctcca gctttttttg ataccttatt tgaaattcct tttcatagat
    22081 ttctacaaaa ctatcgagtt tttttagtac ttctttgaat tcttcagtat tctttttcaa
    22141 cactaaagct aaagcttgac cttctttttc tcgactcgcc tcaatagaat caagtgcgct
    22201 tttcaatgta tctagagcta actgtgccaa ttcttccatg ctctcttccg gtttttcttc
    22261 taccgttacg aagttttcgt ccagcacgaa gcgttccgcc atccgactaa ttgacacttg
    22321 attttgacta ccgtatcgtt gttgaattcc gtctgtcaat tgagtgataa gtgaatcaat
    22381 caattcccag ttgatgacga tctgtttttc gtttttgcca atataagcta gattcacaaa
    22441 tacttcgatt cgaccgcggc ttaactgctg tttaataagc tgacgaaaat cgttttcaac
    22501 aaagttcagg attttaggac ttcgtatttg aacatctaga aaacgctggt tgacactttt
    22561 tatttcaata tctaactgat acgcttttgt ttcgcggatg gctttgccaa aaccagtcat
    22621 acttttcatt cgacagggtc cttcctcatt acatattttc tattgctttt tgcagttgtt
    22681 caaactcttc gttcgtcaat gtcaggcctt tccccatttt ttcatggtct ggcgcccaat
    22741 ctcgaagatc gaattttggc gggcgaccat tccagctgac taggttcagt tctttacgcc
    22801 accccttgtt gttttctgac aatatagcga tttcttctac gatttcatat gaaaattctt
    22861 gtgccatggt tgttcctcct tgagataata taaatatagg atcagtaatg tgtcaactgg
    22921 ttgcttaagc aattcatgct gcaaacgatc cttgcgtttt ccttgtagca gataatcaaa
    22981 ataaacagtt ttcgtgcgct cattttgtat tttttgctgg tattttcgat accttcgcca
    23041 ttcttcagta cttaattgac caatttgtgc caataaatca tccggcatac tttttatatc
    23101 ttgttggcag atcatttgaa tcatcatgtc tacttgatag atttcattgg gcagtaaaaa
    23161 atgttttttt gctaaaagtt ctagcatcgc caatccgcat gtctctggta cgaccctgtt
    23221 cttatataaa ttacgcagtt tttgccaaaa ttttatgctt ttgaaaaaag ataattctaa
    23281 ctgaatttct tttgttaagt aagacaaaaa tcctcgccag tatgttcttg caggtttaac
    23341 agaatgaaat gtataccagt tcccatagta atagcctaat cgttttttca tttccagata
    23401 gcctaattgt aaatttaatt ggttcctttg gctatcaaaa agcagaaagc tgcctagtgt
    23461 ccacaatgtt tgacatttcc aatggataaa aatgtctggc atacgaattt ttttcgctgg
    23521 gcctggccct tgcacatcta cgacaaatgc ctctgttgct cctttgttaa tcgcaatatc
    23581 aataggtatt ttgttgcgat acccaccgtc tgcatattta ttttttccga tctttcgata
    23641 agccatagcg ggataaaatg aagcagaggc gagtatccaa tctggtattt cttctgtttt
    23701 tgtctgttgg atatcagtca ctacttccgc tagttctggc agtttagtag acaccgtata
    23761 taaacgtatt ttgggattat gtttgagtaa ggacaaatcc aaattgtctt tgaccatctt
    23821 ttccaatgga gcagaagaaa ccccgccttc aattagtgct gttttcgtca tttggcgtct
    23881 ttctcgatag aagcgttcac gaaggtcttc aactgcagcc atttctggta acgcaagcac
    23941 ttgtcttgtc gacagctttt tccataaatt cacagctttt ttcatatctc cttgtaaaat
    24001 caatgcgccg ttcaacgcac ctaccgaggt acctgtgata atctcaaagt tgatatcatg
    24061 ttccttcagc gcctgccaca caccaatctg ataagcaccg tgcgctcctc ctccacctaa
    24121 aacaagtgct gtatgataga ccagcttttt atttaattgt gtggttcctt gatagccgtt
    24181 tttatgaaaa atacgcaacc attcttctga tagaggagat tgaaataaaa aacaacagtt
    24241 ttctttaata aaaaaacgag cgcaagcttc catataccga aaaaattttt tccattcaaa
    24301 agtttcacct tcaatcaaca gattattaaa aataacatct cctctacttt tagcaatagt
    24361 aaaatagacg gtatattttt tcgatactgc tttaaaagca cattgctttt ttgcagaacg
    24421 ccaaaaagcg tctctacttt cttgatgact ttgaaaaaat ggaaggggtt gttttgcttg
    24481 ctggataggc gaatacagcg acattttctg tgaaaagtaa acttttttta tctccatcgc
    24541 gctctccttt ttcttcaagt ataccagtac gtcataaaaa ggtcatgagt ctcgtattcg
    24601 actttgtaga ttccataaga aaacaattca taaaacctcc tttaaataaa aaaatacaaa
    24661 aatcacaaaa attaattgaa aatgagagtg ttttcaatga aaacgtgatt ttgtaatgga
    24721 attttaaaat aaaagaaaca agctttttta atgcaaatgt tgtaaaataa aagagattaa
    24781 gaggaaataa gaggaggaac atgatgactg agtcagttta tgtacatatt gataccacaa
    24841 gtaatgctgt tttaacaaaa gggctgactg ctactgactt tgcaaacagt atcgtgcatt
    24901 ttcctcaaaa cttgctcttg cttgatccat ctgcttctgc tggagaatat gaatcccata
    24961 cagggctgaa agtaatccgt ggtacagaaa atattcagcg tttcttttcc tcttctcgta
    25021 acaggcgtgg attcgatgaa ttgaagtgga tcgactttac tgatttaacg atgctgaaag
    25081 aattgacacc tttagaaatt tcagaattat tatattttgg tcatatgaaa acacatcttc
    25141 attcgccgtt tttttataaa ctacagaata attttgtata cttcgatctt aatgatcagc
    25201 tgaatcggat ttattatcgc tatttagatg aattttatag gatttttgct agtaaattaa
    25261 gttcgatctt gtcagataag atcaatgtaa aaaaatcttt tttcaaaaaa gaaacacctg
    25321 ttgaaaaaat cagtgtggaa ttattgaaag aaatgcgtaa tttgatgcag gaagggatca
    25381 ttttttcttt tgatcaagtt ggtttgatcg atggtaaata tcgtatccca atctatgtga
    25441 ttgaagatta tatttggaaa ttaaaaggca cccggtatcg agaagaggaa atcatcgcta
    25501 ctttaatcta ctctgtcacc gatcgacact ggcaattggt agaagatgcc gatctttctt
    25561 atctgccatt gaagtagatc tatttcttat caaaaaataa aaaggaaaca agaaactagg
    25621 acatgattct gtctcaagtt tcttgcttct tttttattta cagctcctgt cgtttatctg
    25681 gcacaaaaat cgaaggagtc gtttgttgga tccctaataa aacgatgatc gttacaatag
    25741 ctgtactcaa cccgctggca ccattcatta cgaaagaaaa gagccaagga ttcatccccc
    25801 atagtgcata cgctccccag aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa
    25861 gtgcgccaat caatgcacca aacacgatgc tttttctaat ttgtttcgta ttatcgtcta
    25921 catagccttt ttggacattt gaagccatga gccctgctgc gcctgcaaaa gtataggcaa
    25981 tcgggtactc aatcaacact tgaacgacac ttaaataata aacttgtccc aacggaaaat
    26041 gtaggatgcc ccaaatgaaa cctgccagca tccctgcctt tgtccctctt cttaacgcaa
    26101 aaagagtcaa tggaatctgc cctaatgaaa tagaaaaact gctgccgatc tggatcggaa
    26161 tcaacgacaa caccatcgct aacgctgcaa caatcgttcc ttctacccaa actctatttt
    26221 ttcccataaa aaaacttcct cctttgaata aaaaagtcac aaaggaggaa gcagctttcg
    26281 aaaatagtca aacagctgtt tattcacaat tcctacgctc gcataaccga tacaggtacg
    26341 aagggtttag aaatagattc aatctcagcc caattggact cccctttgtg agtttattta
    26401 ttatttattt tttgtttcaa acaatggact aactggtttg ttttcatgga tacgtttgat
    26461 tgcatctccc attagtccgc caacacttac ttcatcgatt ttgtcgattt tacgatcttc
    26521 tggaagatag atcgagtctg taacaactag acgctcgata gctgaatctt ctattctttg
    26581 taaagcaggg ccggataata ctggatgtgt acatgaagca tacacactag ttgcaccagc
    26641 ttctttcaaa gcattggcag ccaatgtgat cgtacctgct gtatcgatca tatcatcgat
    26701 cagtacacaa gttttgcctt tgacttgtcc aatgatattc attacttctg caacatttgc
    26761 ttttggacgg cgtttgtcta tgatcgcaat cggagatttc aaaaattccg ctaatttacg
    26821 tgcccgtgtg acaccaccat ggtctggtga aacaacgaca acatcatcgc ctttgattcc
    26881 tttttctaag aaataattgg caataagtgg cgcacccatc aaatgatcga ctggaatatc
    26941 aaagaaacct tggatttgga ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc
    27001 tttttgaatc atgtttgcta ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg
    27061 gtcttgacgc gcatagccat aataaggcat gactacgttg attgtttttg cacttgctcg
    27121 tttcagtgcg tcaatcatga tcagcaactc catcaagtta tcattgactg ggctgctcgt
    27181 tgattgaata acatagacat gcgatccacg aatactttct tcaatgttta cttgaatttc
    27241 tccatcacta aattgagtaa cagatgattt tcctaactcg acgccgactg ctgctgcaat
    27301 tttctctgct aatgggcgat tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta
    27361 atgttttgac atggggacct ccactttctt tttcacaact ctagaattct acctaataaa
    27421 tagtagtcat attttttaaa taaatcaagt gattttgcaa aagtcttagt ttagataagg
    27481 gagttttttt gcatatcctt ctttattgac ttgtcttgct ctcgcaattg ccagcgcata
    27541 ttctggaata ttgtctgtaa ttgttgaccc ggcagcaatc gaactgtttt tagctacctc
    27601 gacaggtccg atgatattcg tgctagaacc aataaagctg tggtcgccga cagtcgtatg
    27661 atgtttgttc tttccatcat agttgacgaa gacaacacca catccgacat tgatttcttc
    27721 tcccagcgta gcatcaccaa cataagtcaa gtgaccgact ttagttcctt tgccgatttg
    27781 agcatttttc acttccacaa agtttccgat atggacgttt tcgccaattt cagcttttgg
    27841 tcgtaaatgt gcataaggtc ccacatctgc atgtttcttg acatggctgc tttcaatgac
    27901 tgagttttca atgacgacgt gatcctcaat cctactgtct acgatttttg aatgtgaccc
    27961 gatgacacaa tcagaaccaa tgactgtatt tccttggatt tgtacacctg cttcgatcaa
    28021 tgtgtctgga ccgatttcta cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct
    28081 tacgccattg accatatgca tctggttaat tcgctttctc atgatctcat ttgctttagc
    28141 taacgcgatc cgatcattga ctcccattga ttcttcaaaa tcatccgttt gataagcagc
    28201 aactgtatgg ccttcttctt ttaagatttc gatgatatct gttagatagt attcaccttg
    28261 agcattgttt gttccaactt tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta
    28321 agttcctgta ttgatttctt gtacaagagc ttcttctggt gttgcatctt tttgttccac
    28381 gattttttct acgatcccaa tatggtcacg gatgattcgt ccatatcctg ttgggttttc
    28441 tgcttgtgcc gtcaagatcg ttgcacttgc attttttcct tggtgatatt caaaaagatt
    28501 gttcagcgtt tcagtagtca ataatggtgt atcgccactg ataactaacg ttgttccttt
    28561 ttttccttcc aagaaagaag cagcttgcaa aacagcatgt cctgttccca attgttctgc
    28621 ttgtaacgca tatttactgc gttctcctag ttgtgcttta acttgttctg ctccgtgtcc
    28681 aacgatcgta atcacttcat ctggtttcgt ttcactgacc cgattaatga tatgttctac
    28741 cataggttta ccagaaacgg gatgtaaaac tttatataat tttgacttca tgcgggtacc
    28801 tttccctgcc gccaaaataa ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag
    28861 tttgctgatt tcagtttatc ccagtatacc cattttttca acaaaacaag tgattttctt
    28921 ttatcccagc gttttctgga taaaaaaata gatagaccat aacgggccta tctatttggt
    28981 aaccggtgct ctcctgccaa ctcctcggtc acaagtcaca ctactcataa aacatgaaaa
    29041 gctgtttttt gaaatcgttt cttgtgactt agagttaaac ggtaggctta caacctcttc
    29101 attaaacgtt tttcaatcag ctgaccttcg gtattagctc aataaacggc aaaatatgag
    29161 gagcaatttt tccgtttatt gagctaatac tcaggacagg acgctgattt cacaacctct
    29221 agataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg aaaaaatgtg
    29281 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcggcttt ttcacaacct
    29341 cttattctga aaaataattt cctggcacaa ccgtaatatt tttcgtttga gtatctacat
    29401 ctttgacata aagcagtgac gtataatcat cgatggcgcg atgaccgttg aattttgatt
    29461 cggcaaatac agtgatgcca acaagttctg attcgaattc ttcaatcaag ctcttcattc
    29521 cgttgactgt tccgccgcct ttcatgaaat catccactac aaggacacgt gaaccacgtt
    29581 tcaaacttct cttagacagt tccatctttt caattctttc agaagatcca gacacatagt
    29641 tgacacttac tgttgatcct tctgttattt ttgagtcccg gcgtacaata acgaatggcg
    29701 cattcagata ataggacaca gcttgggcaa taggtacgcc ttttgtggct acagtcatta
    29761 cagcatcgat tttttcacca aggtatttag aagcaatgat tcttccaact tgacgcagca
    29821 gttcaggttc tcctaacaaa tcagagagat aaacatatcc tcctggaagc agacggtctt
    29881 gttctgatag acgttctgct agagattcaa tatatttttt cgcctcttca tatgaaattt
    29941 cagggatgaa caatacacca cctgcagcac ccgggattgt ttccaaggtt ccataccctc
    30001 gttctttaaa tgttttcttg atgattccca aatcttcact gatagaagat ttagcagatt
    30061 cataacgttc ggcaaagctt gttaaggaaa tcaattcatg cgggtgattc aataaatact
    30121 gtgtcatgtc aactagacgt tcactgcgac gtactttcac ctattccaac tccttttaag
    30181 aaattttatc gttttacatt atataagttt ttcttcaaaa attcgagcat tttatgaaaa
    30241 aataagaaaa tagttcggta tttattaaaa taaaaagaag cctataaaaa atttttcgtt
    30301 tttataaggc ttcttttctt tataatttag ttattttttc ttctgaaggc aaccaatatt
    30361 cgtttgatga tatttacaat caggaacaaa gcaataaaga ctaatgtgat tgttgctcct
    30421 ggaggtgtat ctagataaaa cgagctagtt agtcctgaca gcatccctag aaatcctaca
    30481 caaacactga tagcaatcac gacattgaag cttttgccta atcgcatacc gattgctgcg
    30541 ggaagaacca tgattgcgga tatcaaaagt gcaccggcaa tcgggatcat cacagcaata
    30601 gccacaccag taatcacatt gaacatcatc gacatcaaat gaacgggtag cccatccacg
    30661 tgggcggtat cttcatcaaa agtcagaaca tacatcggcc gtttgaataa aaagaacaat
    30721 ataccgatgg caacgaataa gattcctaaa aagatgactt gctcccatgt aatggtcacg
    30781 atcgaaccaa acagatagga ttggatactt gttgctgaat tccctccgct caaattcatc
    30841 aatactaacg ctagcgccaa accaccagcc attaaaatgg caatggagat ttctgaatag
    30901 gttcggtaga tcgttcgcag atactccaat atcacagctg ctaatacaac gactagcatg
    30961 gtcgtaagtg taggattaag gttcaaaaag aatcctaacg ctacccctgc taaagagaca
    31021 tgagaaagcg tatcagccat cagcgattgt cttctgatca ctaaaaacac acctagcatc
    31081 ggggcgattc cggcaatgaa gatagcggca agaaacgctc ttctcatgaa ttcatatgaa
    31141 agcaacgcca ttgagaatcc tcctttctca ccagtcggat ctgtctgtcc gcatactgtt
    31201 tgatatcttc atgatcatgg gtgatcatta gaactgattt cccatggaca tgcgcactat
    31261 gtcggagaag acgataaaat tcatttctgg attcttcatc catacctgtt gttggttcat
    31321 caagaacaaa taagtcaggg tcagtagcaa aaatacgcgc taaactgatt cgctgctttt
    31381 gtccgccaga caattcacct attcgccgat ttcgcatatc ccacatccct acagcttcta
    31441 aagcacgctg tacatgttga tgatcttttt cagagagacg cttgaaccag cggcctcttg
    31501 gatatcttcc tgaacggact aattcgatca ccgtactagg aaaacctgca ttgaaagatg
    31561 caacttgttg aggaatataa ccgatactta atttttttcc ttcttgattt ttctcagcaa
    31621 tctttatcat tccttttgaa ggtttcagta atcctaacga tgctttcacc aaggttgact
    31681 tggctgctcc attttctcca gttaaaatga caaattcacc cgcatctaca tgataagaga
    31741 catcttctag tacaggttca tcatcatagt aaaaggtcaa atcagatatt tcgatataac
    31801 gcaccttaca acctccattc taaaacttaa ttgatacttt cttttaaagc tttcagattt
    31861 ttttccatga cagaaatata attttcgcct gctttgattt gttcattagt caagctttct
    31921 aaaggattca acacttctag cttcactcct gtttcattcg acaatgtctc agctaccttc
    31981 gaagaggcat tttcttcaaa ataaatcact ttgatgttgt ttttcttcac aaattctttt
    32041 agctcagcta agcgacttgg tgtgggttcc tgatctggtg aaacgcccga aatcgctact
    32101 tgttttaagt cgtattgatt cgctaaataa gcaaaagcag catgctgagt gacaaatgtg
    32161 cggtttgttg catcttttag ctcatcggag tatttttgat ccagtgcttc aagctttttg
    32221 atataagcat cggcattctt cgtaaaagtt tcctttttat ctggatattt ttcgcctaat
    32281 tcttcagcaa tcgttctgac ttcttttata gctaataccg gatcgagcca aacatgtgga
    32341 tcgtattcat gtgaatgacc atgcccttct tctgattctt catgttcgtc tgcttcttgc
    32401 gattctaaac ggtcgattcc tttagctgct tcgatcatca aggtctgttt gctatccact
    32461 gaatccttta acgaatccac aaaaaattcc atgtctgaac tattatacac gaaaacatct
    32521 gcatcggaaa tttctgctct ttcttttgca cttggttcga aatcatgcgg ctccgttcca
    32581 gcagggatca aaagtttcac gtttccttca tcccctacga tctcttttgt aaaatcatac
    32641 atcggataaa aagtcgtaac gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta
    32701 tttccacatg ctgcaaacag taaagcacct atcattaccg tagctgtcaa aagatatttt
    32761 ttcatttata tgctcctttc aatgaacaaa tcggaatttt acgatttatt aacgtgaact
    32821 aatgtatcaa aataaaaaaa cacagtcaat agaaatccat caatttctga tttaagcatg
    32881 tatattcttg attaaatcgt aaaatggatg agttgctgct catatctagc catttcacta
    32941 caataaaaaa ctgaagaata ccattttagg attcctcagt ttttcatcac ctattttact
    33001 tcttatattt tattgcaggg ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc
    33061 attatatact ctctgcgctc ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct
    33121 tccactcatc aatgcagcat ctgctccgta cttgatcata cgctctttga tttgatggat
    33181 gactggatgc cgctttcccg ttatatcttc taaactgttt cccatttttt tgatcatctg
    33241 ctgataatct tgctctcgga tggcttcagc aagtccgtgg atgtcttgat gttggatggc
    33301 ttgcgtatct acttctctaa acactgtacg tgtagagaca ctcattcttg gtttgaccaa
    33361 cacaacccag cactgtggca tagaaggtaa tacttggatt tcttcccctt tgccaccgac
    33421 aaatgccgta cttccgtaga cacagtaagg aacatctgta ccgacttcca aaccgatttc
    33481 tgccagttct tctaacgaac agcccagatt ccataattgg ttgatccctc ttagagcagc
    33541 tgcacagtct gtacttcctc cgcctaatcc agctgctact ggaatattct tcttaacaaa
    33601 gatcttgaca ccggttttga tattgtattt attttttagc aataaagcag cttgataaac
    33661 attgttccgt tcatctaccg gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc
    33721 tgttatttct tcaaaaaata gatgatctgc caagtctacg ctcgacatca ccatttccaa
    33781 ctcatgatac ccatcttgcc gtctatataa tacatccagt cctaaattta tcttggctgg
    33841 cgctttttca atcaattcca tcctctcacc cattcgaaaa gtctaagtcg caagtattct
    33901 atcataggga aaaataaaag tatagcttat gtctgcctag tggttgattt tgtcatcgtt
    33961 tcaacagctt tcacagaatt ttctcttcat aatccaaaca agttctagtg attcttataa
    34021 ttctgtgagc acttcttctg ccaaactcgg aacatttcct ttttcttttc ctatcagact
    34081 gagtagctgc atggtagcaa atccgaaatt acttcctttc ctataagcaa aataacgtcc
    34141 ttcccaatga tcgacatatt ttaatttctt ttcataagca gactggaagg aatagaaaaa
    34201 attaccataa ttatatacga tatgcatgac tttttcttga aagaaagaaa aatctgtttc
    34261 tccaacatta gctaatggag ctaagcctag attggcttcc gagtagccca ttttctcgta
    34321 tacaataaac agattggcta acagatagtc agctaattcc tcagattctt taggtaagac
    34381 ccgcaacaaa tcatatcccg cctgcttctt cgtcatcggc tgagcggtga tgaagccaac
    34441 gactttttga tctttacgga caataccgac accgctagat aatacatatg atttatcaaa
    34501 tcgtcccaca gagaagtatt tttctcgtgc accttgaagc catttttgtg aaacttcatt
    34561 tacagcttga tgtaattctt ccggcagttg atcgtaccaa gtaaaatggt atccttcttt
    34621 catcaaacgt tgatattcaa gttgctgact cagccgagac aagtcttctg ttgctagatc
    34681 aacaatccct gcttcaccta ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac
    34741 gtagctttct gatatcttat aaaacgcaag ctgatagcct aaacaatctg cttgtttcat
    34801 aaactgattc gttgctgccc gcatcatttt aggattgcca actggatctc ctaaaacaaa
    34861 gcatttgttt cctttgatct gataaccaaa aacgacttga tcttggtcgt cttcttggta
    34921 ataaaaataa ctgtaacctg acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt
    34981 ttccaataaa cggtcaaaac ggtctttttc gtactcactg ccaagcttcg tagtatcttc
    35041 agctaaatat tgataaagcg ccactaaagc tagcaatgag atgcccaaac caataagccc
    35101 tgagaaccac acatcatctg aaggaaacaa aataaaatga tttcctgtta ttcgaccgac
    35161 taaaaagcgt ccgctgtgat agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa
    35221 gctagcatca aagattatag ctccccaaga ataaacgaat tttgttcgat aaaattcttt
    35281 tctggataac cacacagcta acagaatcaa gacatatacc aatatcaact gccaagataa
    35341 cgtacgtgtg attgtattta aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc
    35401 ctttttgact tttactgaaa gtgctctagc aaggccgagt aataaaaagc caaccaatag
    35461 attcactgtc tgatctaaaa aattgaatga gaacggcaat aatacttgaa atagccgact
    35521 gattgcagac agattagtga ttgttgatag aagtaccatc atgatccccg caaaatagac
    35581 agcacttacg agcacgatat gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa
    35641 aaatcgattg attttgactc ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg
    35701 tagaacataa taaaacaacc gataatataa tagccaaact actgctacat cttgccgaac
    35761 agacaattga ctcattccta agatcatcaa aacgtcaaaa gtccccattc cacctgggac
    35821 catcgtcagc attcctatca atgtagcaat catgaacata ggatagatcg aaagcatcga
    35881 aatatctatt ctcatcaact tgccgatgat caaaaatacc gcaagggctc ccgtccattg
    35941 tccaaaggat gccagtagca gaccaagcaa gccttttgga taaaactcga agaacaatgt
    36001 ctttctcttt agataagcaa aaaagaaaat gactggtgct aaaaaactac cgccgaaaag
    36061 ccagatccaa tagcttcgaa aaatactttc tgactgaata aaaaagacat caataaatgt
    36121 aagaaacgac catacagaca aaccagtcag cataaatagt gcgacttttg atacagtggc
    36181 taaaaccatt tttctatttg tctgctttcc ataaaaacta gcccgcagac ttgctcctac
    36241 cacaccacca aaaccggcga gattgttgat cgtgtttgtt acccacgcag caagaagaaa
    36301 ctcttttctt tccatttttg gttttccttg tttttctaag actctgatcg tcacccaatc
    36361 atacaacaac atcggagtga cacccagcag tccagccaga atcatgagca ggatcgtcat
    36421 gcggctttgt tgtcccatcc tttgccaaat atcttgccaa ctcatgccat tagcaatatt
    36481 tgcgacttga tttgccacaa agactaacac ggagccgaaa aaaatcagtt ttaaaatcag
    36541 actatgttcc tttatccatt gcagcatcaa gtatcgttta tttttcacat tttcccattc
    36601 ctaacttgta tacttttgtg tcatacccca atgttatctt atttatttta atttttccac
    36661 tttagacctg ttctttgctt gcgatgatag aaaaagaaag atatcctaat gctaaaggag
    36721 aggataacat gaaagtagga tttatcggca cagggaatat ggcacaagct attattgatg
    36781 gtttattaaa aaaaggaaca gtctcgaaag aagaggtgtt cgtttccagt ggacattttg
    36841 aaaatgcaca aaaatttgct gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag
    36901 ttgcatcaca atcagatatc gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat
    36961 tggaagaact aagagaatat agccagaaag tcttattcat ttctattgct tcaggaaaat
    37021 cattaataga tttagaaaca gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc
    37081 cgaatgtcaa tgtcagcgta ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca
    37141 cggaggaaca gcttcagcaa gctacgatga tttttgaagc aatcggcagc gtctatcctt
    37201 tagcagaatc agatttcagt acattcattg gccttgctgg cagctcaccc gcattcgttt
    37261 acatgctgat cgatgcgatg ggacggactg gtgtactgaa tggtttgtcc aaacagacag
    37321 ccactgagat tgctgcaaaa gcaattatgg gttcttgtca aaaattcctt tcaagtgata
    37381 aaaatccatg ggaacttgta gaccaagttt cttctcctgg cggtacaact gttgcaggtg
    37441 tagttgcctt agaagaagca ggattcatcc cagcaatcat caaaggaatc aatacgacga
    37501 tcgagaaaga tcttgagatg ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt
    37561 acaaaaaaag aagagctgtg gcttttgccg cggctcttct tttgtatttt ttattattta
    37621 gcataatcag ttgcacgagt ttcccgaata acgattactt tgatgtgtcc aggataatcc
    37681 agttcttctt cgattcgttt acgaatatca cgtactaaac gaacagcgtc taaatccgag
    37741 atttcttctg gcttgaccat tacccgaact tcgcgtccag cttgaactgc gtaacttgat
    37801 tctacaccgg cgaagctgtt cgaaatattt tccagacttc ttaaacgacg gatgtagttt
    37861 tctaatgatt cacttcgcgc accaggtctt gctgctgaga gcgcatctgc tgcagctaca
    37921 aggacagaga taacagaagt agcttccaca tctccatgat gagaagcgat cgcgttgatt
    37981 actactgggt tttctttgta cttggcagct aattccgcac cgatctcaac gtgagatcct
    38041 tcaatctcat ggtcaagtgc tttcccaata tcatggagca acccggcacg ttttgctaat
    38101 tggatatctt cgccaagctc tgctgctaag ataccagcta attttgcaac ctcgaccgaa
    38161 tgcttcaaga cgttttgtcc ataactagtt ctgaatcgca gacgtccgat gatcttgatt
    38221 agatctggat gaagtgtatg agcaccgact tcaaacgctg cctcttcccc gtattcgcga
    38281 atacgttcat ccatttcttt acgagatttc tcgaccattt cttcgatacg cgctggatgg
    38341 attcgtccat cttgtatcag tttttccaag gtcatccgtg cgatttctct tcggattgga
    38401 tcgaatccgg aaagaacaac tgcttccggt gtatcatcga tgattaaatc gataccagtc
    38461 agcgtctcaa gcgttcgaat attacgacct tctcgtccaa tgatacgacc tttcatttca
    38521 tcattcggaa gggtaacgac tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt
    38581 tgaatcgcta atgataataa gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt
    38641 tcagattctt ttaccattag agttaactca tgattcagct cttcttcagt cgatttcatg
    38701 atgacatctc gtgcgtcctc ttttgataaa ccagcaattt tttctagttc tttttgttga
    38761 tcttcaatca ttttttcaac gtctttttct cgctcttcaa ttaattgctg ttttgaacta
    38821 agtgcttctt ctttactttc aagtgagttc tcgcgttttt ctaacgagtc atctttacga
    38881 ttcagcgttt gttcgcgttg aatcaaacga ttctcttgag attttaattc taacttactt
    38941 tctctcagct cactttcaac ttcagaacgg tatttctgat tttcttcctt agcttctaac
    39001 aatgcttctt tttttagagt ttctgcttct ttttttgcac tatcgatgat tccagcagct
    39061 gatgattgtg caccagctat ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa
    39121 ccaagaccga caattaaacc gatgatagcg aggagaatgt tgaataccat aaaatccacc
    39181 tccatactat ctttttcatt tttatcatct atacagcttc tatgataaat tattctaaac
    39241 tcgtcaatat gcatcttcgc atatatgtgt acaaaaaaca tgcacaactt tattctaatg
    39301 tttgtacccc gtagtgtcaa ctttaaataa acaaagaaac ggctttctac ttattcatta
    39361 aacgatggtt tttaaaataa atgacactag tttcatatag gtaaaccttt aaattgagaa
    39421 acttttctat taaattattc tttgtagatg aacagatatt tttattttat gtacccactc
    39481 gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt caaggagagt
    39541 acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct actaaaggca
    39601 attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata ccataagctg
    39661 ctctaactag agcagaaact tctgccatca tttcaggatg attagccata tagattttag
    39721 cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca ccgcttttat
    39781 caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag atccctaaac
    39841 catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt tttacgactt
    39901 tgatctttgt acggttacca acaatatctg taccttgttt caattgttct gcacgacgta
    39961 cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg atttctggat
    40021 tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg attgtttttg
    40081 ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt gcttgtaacc
    40141 cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa gcagcaactg
    40201 aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg atctctagac
    40261 cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca cctaattttt
    40321 gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt ccgccatttt
    40381 tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat
    40441 atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca tctaacgcaa
    40501 gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat ttcataatag
    40561 agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct gctttacgat
    40621 catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt tcattatcct
    40681 tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc
    40741 attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca ttatatctga
    40801 tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct ttagatgaaa
    40861 tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca gccactccgg
    40921 tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc atctgctcca
    40981 cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc tccagaaaac
    41041 cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca gatacaccag
    41101 caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct gctgttacag
    41161 tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta tcatcgccgt
    41221 atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct aaaagagctt
    41281 gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact tcatttggtt
    41341 tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt agctcagtga
    41401 ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt ttttcttcag
    41461 aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt ttcaactcat
    41521 taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa gtccctaaag
    41581 caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac tgttgcagat
    41641 tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt ttgtatcctt
    41701 ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat
    41761 cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt tcagctagag
    41821 caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa taaacttcga
    41881 tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg acttgtccta
    41941 gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc tcccttatat
    42001 agtatacgct gtatttttat atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa
    42061 atcttgtcac agctccttgt tccaaataaa cagtgttctc ctgccaaaac ctcggtcata
    42121 agtcacaata tacataaaac atgaaaagct gttttttgaa attatccctt gtgactcaga
    42181 gttagacggc aggctcacaa cctctccact aaacgatact tacattgatc ctttgaacac
    42241 accgctgttt ttgatgaagt aatccacacc tgaatagata gtaaagatca gacaagcata
    42301 aagcatgatt tgatctactg gaataccgat caggctgaat gggatattat tgataaacag
    42361 caagataata gcaaccattt gtgtagccgt cttgactttt cccggccaag ctgcagccat
    42421 gacttctcca tgttcgacaa gtagtaaacg caagcctgtg acggctaatt cacgacagac
    42481 aatgatcgct actacccaac caggggcctt tccttggtca accaacatga taaaagcagt
    42541 cataactagc attttatctg ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc
    42601 tcgcgcacgg gcaatttttc catctaacca gtctgtgata ctagctaaag caaaaataac
    42661 tgcacctact agctgtgtga cagccagtgt ggtgtctcct acgttaaatt gtccccaatc
    42721 taaaggtaaa cctacaacca aaatgaaaat cgggatcata caaattctaa gtacagttaa
    42781 tttatttggt aaattcacac tatcgactcc tttcttccct attctacgtg actatcagcg
    42841 aagtttccac cgatattatt tacttttcca aatgaattta ttcaaaggag tatttttatt
    42901 gaaattgatc tttttgaatt aacgaagaag gaccgcgaca cagatttgtc acgatccttc
    42961 ttttttatac ctacaagtaa acagatgcgt aaactaattt atacgatcat tctgcgtatt
    43021 gaagagtaag gttcacattt tttggactct gattgttttc ccctggatta actggtacgg
    43081 attctccatt cgctttgatc gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg
    43141 ttgctgtttc cggtaacacg gtactctgtg tttcatttgc tgccagtgtc ccttgataaa
    43201 cataggcatt gtctactagc acacctaacc atacacgatt gacagcagta aattcaaggg
    43261 ttacaggctt ggtagcgtct gtgatattta ctgcgatggc actgccagaa tcttctcctg
    43321 tagctattgc cattttcttt tcttcacttg attttgttgt tgattctact gtagaagact
    43381 ctttggtctg cgtgctttgc tctgaggaag aggactcctc gactgatcct tctactttaa
    43441 gcgaagaagc tgtgtctccg attattggat cagcttgacg atcttgccaa gtcatatagc
    43501 ccacgacaac gacaatcgcc aaagcaacca atcctaataa aatgacagga agacttgtcc
    43561 aaaagcgact tggatgcgat tcttcctgat gcattgcttt tctagaagta cttactgttt
    43621 caggctgcgg acgttcaggg accgatcctg tcaatttgct tttcccatca aaaacatcga
    43681 ctaaatggtc tccatcttct cctacagctt cagcgtattg ccgaataaat gcccgcacgt
    43741 aaaatttacc aggcaattgg tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    43801 gaatttttgt aatctgctgt aattcatcta aagatatttt tttattcagg cgtgcttgcc
    43861 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    43921 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg ggtgctgtga caaaagtcat
    43981 gtcacagctc cttttttccg aataaacatt gttctcctgc caactcctcg gtcacaagtc
    44041 acaatatcca ataaatataa gaagctgttt tttgaacttg ttccttgtga ctcggagtta
    44101 aacggcaggc tcacgacctc tttcttaaac ggtgttcaat cagctgttcc tcggtattag
    44161 ctcaaaaacc ggcaaaatat aaggagatat ttttccgtat gtttctctaa tactcaggtc
    44221 aaaacgctga tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    44281 taagtccaac caagttaaaa acattaagaa gctgttttta cttggctgtt cttattactt
    44341 gatgcagggc agctttttca caacctcttg gtacacggtg ttccagaagt agcttcttcg
    44401 gcaataagcc gaaatatcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    44461 cttgaagctg accacttctg tcacaacctc tttgattact tcgtttgatt ttattttaat
    44521 ataagttgat taaaagagag catgatttgt tttattttct cactaatttt tccaacacaa
    44581 ctaagaatac cataaaagct gattttggag gaggttccta ctacaagtct aaaacgaaat
    44641 ttaagacttt tcctcataaa atactggttt attttggtct catgtaaaac tcgctgaagg
    44701 cctcttcact tataaatgct tccccagctg ccagtacgtc tttcatttgg atcgaatcga
    44761 tgatttcagg aagatcaaat aaagttcgat cgccaaataa actttgcgta aattgattgg
    44821 caatatattc gatcgaattt aatgattgga aatattgtcc aagcattttc ttcttcagca
    44881 aatcgagatt tgtttctgac acttccggat catcagcgaa tccaagaatg atttctttga
    44941 ctttttctgc tgctttctct ggttcgtccg tatctccgct gaagtccgca aagtgaaatt
    45001 cacgatccaa gctgaattca aaaccaaagc tgtcatcgat aattccttga ttgtacatcg
    45061 ctagatagtt tcgagaagta ttgcctaaaa tcatttggaa caacagattg attgctgttt
    45121 tatagcgtaa cagttctgtt ccttcttgcg gcaatgtgtc taatccttta ataccaagta
    45181 caaatttatc tcgtgtaatg gctgcttcca atgcactttg tttgatgatt tcttttgtgt
    45241 tttcagggaa ataacgtaca atttcttgct ttggagggaa attttttgct tcttggtttt
    45301 cccgaataag tttcatcaat ttctcaggct ccatcttacc aactacaaat aaaaccatat
    45361 tgcttggctg ataaaacgtc cgatagcacg tatacaagtc ttgagctgtg attttgtcga
    45421 tactttcaac cgtgcccgca atatcgatat gcaatgggtg tgtaggatac aagttattta
    45481 gtatgccaaa aaacatccgc cagttagggt cgtcttcata catttggatt tcctgaccga
    45541 tgattccttt ttccttgttt acagtttctt ctgtgaagta aggtgcttgt acaaaatcga
    45601 tcagagtcgt cagattcttt tctacctgat ccgtcgtaga aaacagatag ctggttttcg
    45661 taaaacttgt gaaagcattg gcggaagctc cttgtttccc aaataattga aagacatcac
    45721 catcttcttt ttcaaacaac ttatgttcta aaaaatgggc aattccatct ggaactttca
    45781 ccttttcttt ttcaccatag ggaataaatt cgttatcgat cgaaccataa ttcgtactga
    45841 acaaaccata tgtcttgtga tagtcatttt taggcaacaa atacacggtc aaaccgtttg
    45901 gcagtacttc atgataaagt gtttcattga tttgctcata ttctgtttta ttcatttgca
    45961 tttcctcctg ctaaaaagaa gattgcctgc aattcgattt gctcagctac ttgttgtatc
    46021 tctttgatcg ttacgctctc catacgtttc aaccattctt catcagataa ttttgtttcc
    46081 ggcagccagc tgtccaaaaa agaagcttcg attgccgctt gcggagagtc tagagaaagc
    46141 agatattggt ttctcagcat tgcttttgtc tgagacagtt ccaactctgt tatttcacca
    46201 tttcgcaaac tttctaattg ctcgtgaatc agatgaagta cttgatttcg gttcttttca
    46261 tcgataccgg tttggacact catgaatcct cggaacgtat ccacgctact agaggcataa
    46321 taagctagac tttctttctc tctgacattc atgaaaagtt tagagtgagg aaaaccgcca
    46381 aataaaccgt taaagaccat tagcgcaaaa cgttctggtt catcgtaata cacatttgtc
    46441 tgataagcta ggttcagctt tgcttgaacg attggctcac gtacttgtcg ttccttcacg
    46501 atattcactt gaggttgggt ataaaacaga tctggataaa ctgtttcccg cgactcaaat
    46561 ggtaattgcc caactgcttc agctgctttg tccggatcaa catcgccgac aacaaaaatg
    46621 tcgatttgat catttgccat catttcttga tagtaggacg ctagcgaact agcagtgatc
    46681 ttgtccaaat cttcgactac gcccaaccct gggatctttt gttccgggct gttttcaaaa
    46741 taaagttctt gcaaggcata ggaagcaaaa gtttgtttat cttccttcaa actttccaaa
    46801 tatagacgca gattgttctt ttccaagtca aacgtcaact gatcaaactg atgattttta
    46861 atatttggat aaaataaaac ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga
    46921 tcattgacat attcaccatt cacaaaactg atccctgcgt ttagccaatg taaatttcct
    46981 ttttttctaa ctgataatcc aaagcttgcg ccataaagct ctgcaagttg cgcacttaac
    47041 tttgtctgat ccgaataatt caaactattt gtttccaaca tgctgctgag aagtgaacgt
    47101 ttcgtgatga cttcttgttg cagacgcgca gtaaaacgga taaaaatccg gatcgttttg
    47161 tattgttttg ttggtaagac atgaagattc acgccttctg ccaattgata caccatattt
    47221 atatgctcct tttcacagtt gattgcccgc ttactctttt aatgggtcaa cctgcctttt
    47281 ttacttattc ctctgcttga aatttataag ttcaggcaat tcgccgtttt ttctaaatga
    47341 tgttacactt tatagtatag catagatgtt ttttatcaaa agaaacagac ggctaaaagc
    47401 gtatattgtt tttttatttt ttatttttcg atatactaaa gacaaataaa agaagggagg
    47461 gtcattcatg atcaaagaat tcaaagagtt catcatgcgt ggagatgtct tggatcttgc
    47521 tgtcggcgtt gttattggta gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt
    47581 gatcacacca ctaataggtt ggtttgtcgc aatgatcaca ggtacttctg atttagaagg
    47641 agcactttca attcttgact ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt
    47701 tattagtgct atcattacat ttctgatcac tggttttgtc ttattcttag ttgtcaaagc
    47761 ggcaaaccgt gcgaagaatt tacgctccaa agaagagcaa gtggaagtgg aagaaactcc
    47821 tactgcagaa gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga
    47881 caatacacat ccagtagagc aaacaaatct ttccgatact tctaaataat aaagaatcta
    47941 aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt ttagattctt aacttgctac
    48001 ttgttccttt ttcatgtcga tatgtggaat atcatcttcc aaatacacat ctgagattgc
    48061 cttgaatcca aacgactcat aaaaagcttg taggtattct tgtgcgccga tcacgattgg
    48121 tttttctgga aaatttttct ggatcatgtt aatcgtttct tgaacaatct ttcttcctaa
    48181 accatcttta cggttgtcct gatgaacaag gacacgtcca aaatggatac tctccgcttc
    48241 ttcatatact cgcgtgtagg caaggatctt cccatcagta tcttgaaaaa acgtatgcca
    48301 agcttctcca tcaatgtcat caacttcttg ataaggacac tgttgttcca caacaaaaac
    48361 tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt gacaattctt caaatttctt
    48421 tacctgatat tccactaagc aaacgccccc tgctttttta ttcttagcat accgattcgc
    48481 acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt ttgcttttta aactgcttca
    48541 gttaaaaatt cgatctcaac tgtccgagta agtacatctg aatagctgta agatactcgt
    48601 tcaaaagaat tttcttcagg atccaaatca acaacgaata ctgaaggata ggtttctgtt
    48661 aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct gagcaaccaa catgattggg
    48721 ctgccgatac gaccttctaa atctttcttg attgaagcta aagttgttgg catacagttc
    48781 acctctacta tcaactatac cacatatttt taaaattttc aagtttacca taaaaaaaaa
    48841 agaaatgcaa taaaaataaa taagaagctc attaagattt ttctaaatta ttcctcatta
    48901 taaaatttca agtgtttttt tattttgagt tttactctcc cgtaagcgct cattacttgc
    48961 ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat caacgccttg catataataa
    49021 tgaaaaattt tagcttctat ttttgaaaac tggatttcgc aattttcgat tgcttctttt
    49081 aataaaagat gctgaattgc taaaggttct tgcaatgtga tctctgctaa aaagtctggt
    49141 ccttctgtct ctattttttg gtcgattgag acttcatctt tttgatcacg acgcttcagc
    49201 gcgttctgct tcctcaacag agaatagatt tgattgctta taacgctttt ataataactt
    49261 ccaaagctaa cacctttgct tttttcgtaa gttctcaatg cctgatataa agcgatcctt
    49321 gcttcttgtt cccaatcctc agaaacataa tttcttaaat aataggtgtt tttcaaacgt
    49381 agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg cttcttcgtc accattacgt
    49441 gcagcgcgca ataactcctc agacataaaa taattcctcc ataaattcgt ttatggagga
    49501 atcttatcat caatgattca tcgaactgat tagaacgata tgttatttat tttttctcat
    49561 taaatcgtcc aatttttggg acaatttttt taactgctct tcattccatg gagaattacg
    49621 tcggaaatca ataaaatgca tatctttatt ttcttgggct attgtttttt ccgttttttt
    49681 tatcgtctta tataattcat aagcagaagt tcgtaatgct ccttgggaaa agatcatcca
    49741 ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc aaacggtcat tcagctgacc
    49801 agcgattctc tcgatataac tgtcagccgt ttcgtcttct ttcgtaaaaa caacatccaa
    49861 ctggtatttc ttataatttt gttggatgcc cggaactagt tgggcatcga agacaacgat
    49921 gacttccatt ccttcatatt tcgcataatt tgataaacga tgtaaaagag cttctcttgc
    49981 atccgccatt ttgtcctgct ttttaagctg gacaagttcc ggccacgaac caatcatgtt
    50041 gtagccgtct acaatcaaca gctgtttttt catggtctac tccccctgac gttgggaaaa
    50101 agcctgatac ataagcaaac cagcagcaac actggcattt aagctctgga cgtgcccggt
    50161 catcgggatc gtcagcaatt catccatttc ttttttcaat cctggactca ttcctttgcc
    50221 ttcattacca atgactaaag caattgatcc tttggcattc catttttgga agtttgtgcc
    50281 ggtcatatct gtgccaaata cccaaaatcc tgtttctttg agctgttgca cagcttgtac
    50341 caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc ccagtggaag tctttgttac
    50401 gataggtgtg attccaactg ctcggtgttt tggtatgatc acaccatcga cgccacttgc
    50461 gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct tcgatgctat ccaaaatcaa
    50521 aaagaagggg tcttcttttt tctcttttgt tcgttcaatc agttggtcaa gggataaata
    50581 ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg ccgtgatcgc tcaaagtttc
    50641 taatttttgt ttaggaaccc atttgactgg cacagcttgt tcacgtgcga tttctttcag
    50701 ttcttctact ttttctcctc gtgaatcttc ttgaagaaag agtttattcc ctcgcccttg
    50761 ttgtagcgct tctactgcgg catggtgccc aaagacgaaa ttatcttcta atgcttcttc
    50821 attttgatca gaaactgact gatttcgtcc agtttgcttt ttatttgaat atctattttg
    50881 ttttttgtct tcaaatccac gtttcgattg atgtttgttt gttctttctt tacccatttt
    50941 tttcaccaac tttctgaata caccagcgga tcaattcttc catccgctcg gtttgttttg
    51001 tcaaatgcag atagcccatg agcgcttcga accctgttgc gacccgatac gtcgtaacat
    51061 cagcattttt tgcgctcgta tggcttttag catttcttcc tctgcgataa taaagttctt
    51121 cttcttctgt caaaagatgt tcttccagca tttgctgaat caaaaaggct tgtgcattcg
    51181 cagaaacata attcgttgcc gcacgatgca gcttattggg ttttgtttct ccttgatcaa
    51241 tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc aacataagcc aaggctaaac
    51301 cgttcaattg tgtgtaatct ctcatgcttc tcttctccat cgggttcctt gcggtgtatc
    51361 ttctaataag atcccttttt ctttcaacaa atcacgaatt tcgtcgcttc ggacaaaatc
    51421 acgattgctt cttgcttgat ttcgttcttc gatcaattgt tcaatctctt catctaataa
    51481 ctcttctgct gtaaataaaa taccgaagat cgaaagccat tgcgagaata aagcttctcc
    51541 ttgtgccaag acagcggcag aaactttcgg ctggtctaga taagtgttca gccacttagc
    51601 aagctcatag accactgtga tcccatttgc tgcattaaaa tcatcgtcca tttcttcgat
    51661 gaatcgctgt tctaaatttt ctaacaaagc gattttatcc gcatcatctg ccaaattctc
    51721 tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta ttcttcagtc gttgtaaatt
    51781 ggtggctgct tctttcatcg tcgtttcact gtagcggatc ggacgacggt attgtgtggt
    51841 cgccatgaag aaacgcaaga cttgtggatc aacgtttttg atcatttcat gtacagtgat
    51901 gaaattccct agagatttac tcattttttc atcgtcttcg ccaatcgtta catagccatt
    51961 gtgcatccag taattagcga attttttccc tgttttcgct tcactttgtg cgatttcatt
    52021 ttcatgatga ggaaattcta aatcttgccc gccaccatgg atatcgatcg tttctcctaa
    52081 atgttttgtt gccataacgg aacattcaat atgccaaccg ggacgtcctt tgccccatgg
    52141 tgaatcccaa gaaatctcgc caggttttgc ttgtttccat aaagcgaaat ctaatgggtc
    52201 ttctttttgc tgctgttctt cccctgtacg ctgacttgct ccgacttcca gttcatcgat
    52261 ggattggtca cttaatttcc cataattttc aaatttacgg gtacggtaat acacatcacc
    52321 tgaagattca taagcatagc ctttttcaac caacacttga ataaatgcta aaatatctgg
    52381 catatgatcc atgactcttg gatgcagtgt agcaggttcg acattcaacg ctttcgtatc
    52441 ttcttcaaat gctcggatga aacgatcagc aacttctggt gctgtaatac ctaattcgtt
    52501 tgctgtacgg atgattttat cgtctacatc cgtgaaattc gatacgaaat cgactttata
    52561 gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca atggtgctac gagcatttcc
    52621 gatatggata taattataca cagtcggtcc gcagacatac ataccgacat gtcccttttg
    52681 tatagggatg aattcttctt tttttctggt caatgtattg tagattttta tcattctgat
    52741 ctcctcactt tttctccttt gattcgaacg acttttgctg gtactcctac cgctgtagcg
    52801 tcgtctggaa catctttcaa cacaacggca cctgcaccga ttttggcgtt ttttccgatc
    52861 gttaccggtc ctaatatctg cgcattcgca gagagcatgg ctccttgttt aacagttggg
    52921 tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta cgccatggaa taaaatcaca
    52981 tcgtcctcga tttctgcggt ttcgccaatg acgattccca ttccatggtc gatgaacacg
    53041 ccagttccaa tcgttgctcc tggatggatc tcaacaccag ttaggaaacg ccagaattga
    53101 gcattgatct tcgccaataa aaagagccgg tgctgatata aaaaatgaga gaaacgatgc
    53161 caaaacaagg catgaaaacc agggtatgtc aaaacgatct cagctgtcga acgtgcagcc
    53221 ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc aactcatgac tttccactcc
    53281 tttcgtaaga attttatgta ggaaatcatc ttcttataaa gaaaagaagc gccttttaaa
    53341 taagtttcct tatcaaaaga cgctttccgc gtggttccac ttttcttcat aatcaatgat
    53401 tatctctgac agctaacgcc tgttatacgt tcttggctac tttgtttcct ataaaacagt
    53461 ttttcctgtt ttctgtgttt cgccaagacc actcccagag gcatttcggc aaccattcat
    53521 tgataccttg caccacccgg tatctctctt gtaatgatca gctgcttact tttttctgtt
    53581 catcgtgttt agttcagtac ttgatttaaa tgtgctaatg atttttcacg accaagcaat
    53641 tcgatcgttt caggcaattc tggaccatgc atttgtcctg atacagctac acggattggc
    53701 atgaaaagat tttttccttt tacgccagtt tctttttgaa cagctttgat ggcagctttg
    53761 acagttggag catctactac ttccatttgt tctagctgtg ctttgaatgc gtggagaaca
    53821 gtaggaactg tttcaccagc aagtacttct tttgctgctt cattcaatac aggatgttca
    53881 ttgaaaaata gcgtagacag atcaacgatt tctgcagcat agctcatttg aggttgatac
    53941 aggctaacga tcttcttcac ccattcgatt gtcttttcat ctgggttttc tgctacacgt
    54001 ccatcagcga ttagataagg cagacacatg tcagtaaatg catctagatc cattgctttg
    54061 atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga aagctgcggg tgatttactt
    54121 aggcgttttg gatcgaacat tttgatcaat tcttggtgtt cgaagatttc gtcttcgccc
    54181 acaggtgacc agcccaataa tgcaataaag ttgaacattg catctggtag atagcctaat
    54241 tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac gtttgcttag ttttttacct
    54301 gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg gtggtgtcca gccaaatgct
    54361 tcatagatca tcagttgttt tggtgtattg gcaatatggt catctccacg taaaacgtga
    54421 gtgattttca tcaaatgatc atcgaccgca acagcaaaat tataagttgg cataccatcg
    54481 cgtttttgga tgacaaagtc gccaccgata ttatcggatt cgaacgaaat agcgcctttc
    54541 accatatctg taaatgcgta ttctgtattg cgaggcacac ggaaacgtac aacagattct
    54601 agaccttgtg cttctttttc tgcttgttct tcaggtgtca agttcgcaca tgttcctgcg
    54661 taatgaggca tttctcctct tgcgcgttgt gcttcccgtt ctgcttctaa ttcttcttct
    54721 gtgcaatagc atttgtatgc tcgattgcta gcaagcagct gatcaatcag cggttgatag
    54781 atttctttac gttcagattg acggtaaggg ccgtattctc caggattttc aggagattca
    54841 tcccaattca ttcccaacca agcaaggttt tccaactggc ttttttcgcc atcttcgata
    54901 ttacgttttt ggtcagtatc ctcaatacga ataataaagt ctccatcatt gtggcgggca
    54961 aacaaataat tgaataatgc agttcttgca tttccaatat gcaggtgccc agttggactt
    55021 ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct tctttcaaac tagactttga
    55081 ctaacagcaa aaattccgtc agttacggct tctcaaatat catttattta aagccactca
    55141 gcacaaattt taccttttct aaggtaaata gtaaagccaa ctggttgaat ttccttacca
    55201 gttggctgaa tttctttttt attttgattg tttagtcttt ttctctgtct cttttttatt
    55261 gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc atgcgtccag cagctgtttg
    55321 aagggcactt gttacaacga cttcgatacg ttcattcatg taatgctgtc catcttctac
    55381 gacaaccatc gtgccgtcat caaggtaggc tactccttgt tggcgttctg tcccggcttt
    55441 tactactaga acattcatcg tttcaccagg gatgacgact ggttttacgg catttgccaa
    55501 cgcattgata ttaagaacag gtacattttg gaattcagag actttattca agttatagtc
    55561 attggttaca atgacaccat ctaaaagctt agccaatttg atcaatttgc tgtctacttc
    55621 actgatatct tcaaagtctc catcgtacat ttcgacagat ataccctctt ctttttgcaa
    55681 ggcatttagg atatctaatc cgcgacgacc acgcacacgt ttcaaactat cccctgagtc
    55741 cgcgatgtat tgtagttcgt aaagaacaaa attagggatc atcaaggttc cttctaagaa
    55801 tccagtcttt gtaatatcat aaatccgacc gtcaatgatc acgcttgtat ccaagatttt
    55861 atatttatgg aaattttctc ctgattttcg ttctaacagt tgactctcaa cttgactagc
    55921 tgtttcttgc tcgatttttt tgcttcgtga accaaaaact ttcttccatt cttcgattcg
    55981 tgtcgttccc attctcaatc ccagatagcc taagatgatc atcagtaaaa tcggcaatac
    56041 actattgacg aatggaattt ccatattgta aagaggggtc gagatcacaa cacctaaagt
    56101 caatccgatg atcgcaccga tcgcaccgaa gagaagataa gtcaagctga cctcacttag
    56161 tttttgttct acttgtttga taatagctga gatatgtttg gccaacagca aagataataa
    56221 aaagaaaata agtgcgccta acaggctgtt ggtaaatgta ttattcagcc atccgtttga
    56281 ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa atacccaagc ttgcgccgac
    56341 aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt tttcctccgt ttcgacaatc
    56401 atttattagt tagcggaaca cacgcttcaa cgtttcagcc aaagttgcta ctccaactat
    56461 ctcgattcct tttggcgctt cccagttacc taagttgttt ttaggtaggt atacttttgt
    56521 gaagccgagt ttttggactt ctttgactcg ttgttcaata tgactgacac gtctgatttc
    56581 tccagtcagt ccaatttcac cgatgaaaca ttccgttggc tgtgttcctt tttctttata
    56641 gcttgaagca atactgacag cgatagctaa atcaatcgct ggttcattta attttacccc
    56701 acctgcagct tttaagtaag catcttgatt ctgaagaagc aacccagctc ttttttccag
    56761 cactgccatg atcagagata cccgattgaa atcaagacct gttgttgttc gtttggcatt
    56821 tccgaacata gtaggtgtga ctaatgcttg gatctctacc aaaattggac gtgtcccttc
    56881 cattgccaca acgatagcag agccagttgc accgtctagg cgttcttcta agaaaatctg
    56941 tgaaggatta gcgacttctt ccaatccatg ctcatgcatt tcaaaaatcc caatctcatt
    57001 cgttgagcca aatcggtttt ttactgctcg caaaatccga aaactatgat gttgttcacc
    57061 ttcaaaatat aacaccgtat ccaccatatg ttctaacatt cttgggccgg cgatcgagcc
    57121 ttctttggta acatgaccga cgataaaaat ggctatccca tttgttttag cggttttgag
    57181 tagttctgca gtggtttctc ttacttggct gacacttcct gccacacttg tgatgtctgg
    57241 ctgcgtcatc gtttggatgg aatcaatgat aacataatca ggggagatat gttcgatggc
    57301 acgactgatt tcgttcatat ctgtttcagc gtatagataa aattctgtgt tgatacttcc
    57361 gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct tcgccggata catataagac
    57421 ttttccgcct atggctgcta attgctgaga aacttgtaac agcaaagtgg atttcccgat
    57481 acctggatcg ccaccaatca agaccataga ccctggcacc acacctccgc ctaacacacg
    57541 gttcagttct tctaattttg ttttgatccg aggttctttt ttagggacga cgtctgcgat
    57601 ttttgttggt tttgcttttt ctcctgtcaa acttgtccga gttctgcggt ccgttgtatc
    57661 ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa tttgggcagc gtcccaagaa
    57721 cttaggcgac acatacccgc aattttggca aacaaattgt acttttgatt tttttgccat
    57781 tgtaccgcct ccttttatcc taatcatgag acattaaatc aagcttaagt atctttaacg
    57841 ttttatttac tggaagaacc aaatccgcct tgacgttgag cagtcgtttg atcttgatct
    57901 gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct cgcctttttt gatcactaca
    57961 tccttgaaac caaagttggt gaattggaac atgatatggc cttcatttgc ctcattattg
    58021 taataatcac tgtcaatcac acctactccg tttgtcaaca ctaaaaaccg cttcaacgga
    58081 ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc ccatgtaagc tttgattcca
    58141 gtaggcacga gggttggttt caattgtttt tcattttctg atacatcttt tttcaatcca
    58201 tctgcgactg tttccaaccc ctttttccag cctgctttcc agatacttgg aatcacgacc
    58261 tcttctgctg cttcaaaatc atagccagca gcatgatgtg tagcgcgctg cggtagattg
    58321 atattttgat ctttatattt agaaataact tcaaatcctc tttttctcac cggttttcct
    58381 ccctaaataa actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa
    58441 ttcttcaaga aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac
    58501 tgcaagttgt acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata
    58561 gaacgaaatt aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat
    58621 gcaaaacgaa ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc
    58681 aatcttgcgg caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag
    58741 gaagtgatct tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca
    58801 gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg
    58861 atgaatatct ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg
    58921 acaagagacg tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag
    58981 cttttgatta tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa
    59041 ttagatcatc tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt
    59101 gtcgcggcag accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg
    59161 actacaaatc ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg
    59221 gcacaagcag gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa
    59281 gccgcttcta ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg
    59341 gcaattattt ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg
    59401 aaatgactga cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac
    59461 ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga
    59521 atcaacaagc gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata
    59581 tcagtgaaac gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta
    59641 cagagatcga aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga
    59701 cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg atcggcatca
    59761 tttacgaatc tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga
    59821 atgccgttat ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat
    59881 tattgcagca agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg
    59941 atacttctca tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga
    60001 ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca
    60061 ttgaaacagg tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg
    60121 cagtgaacat catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa
    60181 cattgctgat ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg
    60241 tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg
    60301 ctttagcaga agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag
    60361 tggtcgattc tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag
    60421 aaacgatcgt gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg
    60481 cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg
    60541 cagagattgg tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat
    60601 taacttccac aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt
    60661 gaaacattat tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg
    60721 tcaatatgtt tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg
    60781 cttttctggg agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga
    60841 gtcaacggga aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac
    60901 gcttgcacat tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt
    60961 ttttcgatca ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat
    61021 cttataaata tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg
    61081 tacgataagt agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg
    61141 acaaagattt tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg
    61201 gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat
    61261 aacgaagcag ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt
    61321 cagccagtag ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt
    61381 ttctagtttc accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca
    61441 accgatcatc tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat
    61501 attcttccag catctattat ccccccattc tttttcttaa atgaatttta gcttacctac
    61561 tgaaaatttt ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca
    61621 catccccttt ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat
    61681 attcaacaaa catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga
    61741 cagtctcaca acctcttggt aaacggcgtt caatcagctg accctcggta ttagctcaaa
    61801 aaacggtaaa atatgagaag ctatttttcc gttttgtttc tctaatactt aggataaaac
    61861 gctgatttta caacctctgt taaacggtgt tcaatcagct gtccttcggt attagctcaa
    61921 aaaacggcaa aatatgagga actatttttc cgtttgtttc tctaatactc aggacaaaac
    61981 gctgatttta caacctcctg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    62041 caaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag
    62101 gacagctttt tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg
    62161 gtgataaatg atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata
    62221 gctttcacca atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa
    62281 aaagcggaca aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat
    62341 agcaatcgtc gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct
    62401 gatcggtaca aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa
    62461 taagacaaat gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc
    62521 aacgaaaatc gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc
    62581 taattcagaa tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa
    62641 attatataac aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac
    62701 accttcccaa aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag
    62761 ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc
    62821 actgcccaag accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc
    62881 atacatcatg attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc
    62941 agtagagaca aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc
    63001 gatattccca gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat
    63061 caatgttttt ttcaaaccga tccgactaga taagaaacct gtaaatagca tcaccacacc
    63121 agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag cgtttaactg
    63181 tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag cgatcataca
    63241 ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg tttgttcttg
    63301 tatatctatt tgattctcca atatttccat ccctttctct ctattagcat catacatgat
    63361 ttattttaca tgtcaatatg cacatgtaaa acgtggaaac tttactgtga tataataaag
    63421 aaaataagac gagaggtagg atttttatga atactttagc ctatatatta ttatgcgtgc
    63481 ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt ttttgggaag
    63541 cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt tatgtgaccg
    63601 ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat gaaggacgtg
    63661 ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc
    63721 tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga ctgctattca
    63781 ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct
    63841 cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga attatgcttt
    63901 gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag ttttttgagt
    63961 aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc ttcaataaaa
    64021 gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg
    64081 atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc caaggccttt
    64141 tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac acttcttgaa
    64201 agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat tttccaagca
    64261 ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac accgaataaa
    64321 gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat catttggacc
    64381 ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac accgatgatg
    64441 aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa caaacttgat
    64501 aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac catcaaaaaa
    64561 cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct gtaccactcc
    64621 tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa gtactttttt
    64681 tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa agtaagaatc
    64741 atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta aagcttcgat
    64801 cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac ttggcttagt
    64861 aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac ataacggttt
    64921 cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa gcgattctga
    64981 aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca tttgataacc
    65041 cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt ctccttgttt
    65101 gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt
    65161 tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac ttctttccaa
    65221 ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt ttggtactgg
    65281 aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt ggagtcttct
    65341 ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt gtttagagga
    65401 agacaaccga tccagtctga tttgttgctt tttcttttta ctggacactt tttccaactt
    65461 ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct ctttcatgat
    65521 cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga gataggcact
    65581 ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt tcgatgctag
    65641 ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat aataacgaag
    65701 ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct cgtctagaag
    65761 catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt cttcccctcc
    65821 gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg ctaattccaa
    65881 atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga aggtgatctc
    65941 tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga gcaaagtcga
    66001 tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga cacgaagaga
    66061 atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta tatttactgc
    66121 taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat cgatactaaa
    66181 aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga
    66241 aataactatg actaaatcac caaatctgtc atagctttca cctattctac tgtcgttcga
    66301 aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg aatttattta
    66361 gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc cgacctcctt
    66421 ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata gtaactgaaa
    66481 accttttatt cgaacatagt gatcttttct ggtgctaaac gaacagactc gtaaccttct
    66541 tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt atccaaacca
    66601 aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc atagccatga
    66661 tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt gactacatcg
    66721 ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa ttgttgatct
    66781 ctgacttctt ttggcatctt ccctgattcg taagcttggt tgtagatttc ttcgccatat
    66841 tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc attttgtctc
    66901 gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc agcgaccaca
    66961 aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc
    67021 agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga tcttctacca
    67081 gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt tcgtttctgt
    67141 tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt gtttcatttc
    67201 tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca tttcacaatg
    67261 cttgattttc ttctgggctt tttctgttag cgctacatac acttcccggt tgttctgagg
    67321 atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac gagtaactgc
    67381 tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat tttcttcttt
    67441 cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt caaagatctg
    67501 gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct cttctaaaga
    67561 cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa taatagaata
    67621 aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc aatagaaaag
    67681 tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa agcacaacgc
    67741 taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg cagtagataa
    67801 ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa
    67861 attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg tcacaacctt
    67921 ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac caaacaaaaa
    67981 acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca gctttttcac
    68041 tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca tcggtgttag
    68101 ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt catttggatt
    68161 aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt cataacgcca
    68221 accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat ggtctccact
    68281 gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc aagcaatccc
    68341 ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca atgtatataa
    68401 atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag ttggtgctgt
    68461 atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac cataattagg
    68521 cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt tcgtattgct
    68581 tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt
    68641 tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg cattcaccgt
    68701 aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc cattatagct
    68761 tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt tcccccagcc
    68821 actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg cacttgccaa
    68881 actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag cagatgagcg
    68941 agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt gatgggtatg
    69001 tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct ctggattgtt
    69061 ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg
    69121 ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac tttctgtatc
    69181 gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt ctgtcgattc
    69241 ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa cgctaaataa
    69301 taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc taaataacag
    69361 tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt gtttcttttt
    69421 aataaatttg ctttttctgc ataaaaagac gtaaatgaag aatattcttc tccattcacg
    69481 ccttaaattg atggtcgttt gaataactat ttaacaagta attctctctg gatattatcc
    69541 gttatggatt atacttcctg tgtcgtcgga cggataatca tgttgctgac agacatccgg
    69601 tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcagatgt taatccccat
    69661 tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt ttgatataat
    69721 tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg ccgttgctct
    69781 tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata aacggcggaa
    69841 tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc agatttttgt
    69901 ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat gttgatatca
    69961 agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg catgattcct
    70021 gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc gataactgct
    70081 tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc ttctggtaaa
    70141 gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag tttagcccct
    70201 ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc catgatgaca
    70261 atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa atctcttgct
    70321 tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac aggtttgacg
    70381 attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt aacagttgca
    70441 ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt tgctcctgct
    70501 ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct tcgttctttt
    70561 ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa tactgaatct
    70621 tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa gacatttttc
    70681 cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt caccgtatca
    70741 tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg ttcatggtac
    70801 ccagtattca gcgctgctaa aggctcttcg ttttccgctt ggatctgatg gatcacttca
    70861 aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat gaggcttgtc
    70921 attttgctcc ctccttttat ccttacttta taacggacgg cttatgatgt ctaatactta
    70981 taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt taaaaacagg
    71041 agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag agaaaaaaat
    71101 atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa acaactgaaa
    71161 gatttagaag aagaacttgg tgtgacactt ttcactcgag gctcccgaga gatacagttg
    71221 acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa
    71281 acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat tggcggaggc
    71341 gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa ggcgcatcct
    71401 gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa actggatagc
    71461 ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt
    71521 cgactgccag cccaagatcg ttggggactt ttgatacatc ggaatcatcc tttagcaaag
    71581 caaacagtca tccatccgca ggaactgaaa actgttcctt tgatcatttc ccggcaaacg
    71641 tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt atcgatcgtc
    71701 ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac ttttggtgca
    71761 ttagctctag atgggatcat caatacttat gggacggaaa aagtattcat cccactcgat
    71821 ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct ttctcctgct
    71881 gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt tttttccttc
    71941 gctttcagct ttttaacaac gaataagtta agcaaaagat ccaatgatta aataacataa
    72001 gtttcttaat ttgttccttc cttttatttc tgataattcc agtaaattac tataattata
    72061 aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa caataattcg
    72121 tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg tttccctagg
    72181 aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc
    72241 aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat
    72301 tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga tttgacagaa
    72361 aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg gaaccagaag
    72421 gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta tttgcagcag
    72481 aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa catgtattga
    72541 tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt atcgtcatcc
    72601 gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt attgctcaat
    72661 gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac gaagcaggat
    72721 atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat cgcagtatcc
    72781 gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg gaaacagata
    72841 tccattcaga accattttca ctgaattatt caagactggt gaatcattta cgactgcttc
    72901 tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta gaaatggtca
    72961 aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag
    73021 aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat atcgagcgtt
    73081 taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa aattgaaagc
    73141 atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac ctgctgctgc
    73201 tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg tcgctcattt
    73261 tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg ccgttgggtt
    73321 agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt
    73381 tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga atatcaaacc
    73441 agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta ttattgccgg
    73501 attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga tggctttatc
    73561 tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt tgctgatcag
    73621 tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt ttgctaaatt
    73681 cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc gtttactgat
    73741 ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag caggaatcaa
    73801 tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc
    73861 tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta tttacagaaa
    73921 tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ttagcagcag cttttgcttc
    73981 attctttact ggtgtaacag aaccacttga attctcattt atgttcgttg cttggccatt
    74041 atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat tcttccattg
    74101 gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc
    74161 gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag tgatttacta
    74221 ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc
    74281 agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg cggcacaagc
    74341 aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg ataactgtac
    74401 tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata aaatcaaagc
    74461 cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac
    74521 agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg caacacgacc
    74581 tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtggaacga tcgaacaaga
    74641 tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag atgatgtatt
    74701 cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag aaatcttctc
    74761 tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg gaatccaaac
    74821 agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact taaaaggcga
    74881 accattcact ttatatgtaa aagaaggtca aaaagtcgca agaggtcaat tgattgcttt
    74941 agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg ttgtattcac
    75001 aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga
    75061 taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac ttttttatcg
    75121 ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg aataaaaacg
    75181 gcgttctcct gccaactccg cggtaataag tccagccaaa cgtaacatat gaagagctat
    75241 tttttatttg actcttctta ttacttgatg cagaacagct tttttacaac ctctgataaa
    75301 cggtgttcaa aaaccagttt atcggtatga agtcaaattt atgcaaaaat gtggaaagcc
    75361 attttcacaa aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata
    75421 cattattcct gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc
    75481 aatacttttt ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg
    75541 atcgctaaaa acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca
    75601 aacgctgcat cacttgcttc aataatttct aactttttac ctgtcgcacg ctctaatgcc
    75661 ttggctaatt cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca
    75721 ctttctacta gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt
    75781 tttccatttc ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt
    75841 aattcattct ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta
    75901 atcatttttt cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct
    75961 gcaaaacttg tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc
    76021 ttatgttcgg cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct
    76081 ttcaacgctt ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca
    76141 gcttctttca gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt
    76201 ggaaccatct tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt
    76261 actaaataat gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta
    76321 catttataaa ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat
    76381 gaaaggatga caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc
    76441 ctatattgaa atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat
    76501 cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat
    76561 ctttacaaaa accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt
    76621 actagatgtc tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac
    76681 aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg
    76741 gctgcagaaa aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa
    76801 aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt
    76861 ataattctct ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta
    76921 ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag
    76981 cgctgaagtc atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc
    77041 tgaaaaatgc aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa
    77101 gcgctacttt cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct
    77161 cccatatatt tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag
    77221 gcgtaatctt ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt
    77281 aattcttcag gcgattctgc taaagcgatt acaacaagat acgttccttt tgggaatggg
    77341 atgattcggg tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt
    77401 ttcccgttat agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat
    77461 gcatctattg ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa
    77521 ccgacaaaat ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct
    77581 ggtttttctt cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca
    77641 tcttacataa aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta
    77701 ttttttaact atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa
    77761 tcctctcccc attttgtaaa agagatctcg ggagcttcga ctaactcata atctgaagaa
    77821 ggaagccatt ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct
    77881 ttattaggaa aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac
    77941 ggattttctt ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag
    78001 cgggctccat caaatccata agaaacgttg attacttgat ttggatataa atctcctaac
    78061 ttatgcatct cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga
    78121 ttgactcctt caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct
    78181 ttcttttcga tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag
    78241 cgtggaaaag atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa
    78301 tcacgaaaag ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct
    78361 gtcactcttg atccatctag gagatcgcga ttagccaaag ccaatctacg gtttttgata
    78421 tagtccatta gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag
    78481 attcctgtat tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg
    78541 taatcgatca attggttcag atctttaagc atatttatct tcctccttgc ttgccttttt
    78601 atcatattat caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta
    78661 gaaaatgttg tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt
    78721 attatctgcc tatctatgtt ttacctttag aaaaataaat tttggggaag acacattcta
    78781 agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg
    78841 tacaaagcaa attgttgttc aaactgctga tcagaacgct gcttggattt ttctttgcga
    78901 tacatcatac ctcttttatc aattggaaca attatttatt tctaacaaaa tcacttcatt
    78961 tttgtcagat aaataaaa
//
LOCUS       DANJJH010000004        53151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000004 DANJJH010000000
VERSION     DANJJH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            418..840
                     /locus_tag="PWS53_000268"
     CDS             418..840
                     /locus_tag="PWS53_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0870543.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            837..1067
                     /locus_tag="PWS53_000269"
     CDS             837..1067
                     /locus_tag="PWS53_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870544.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1565..1765
                     /locus_tag="PWS53_000270"
     CDS             1565..1765
                     /locus_tag="PWS53_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0870545.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1792..2985
                     /locus_tag="PWS53_000271"
     CDS             1792..2985
                     /locus_tag="PWS53_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0870546.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3053..4618)
                     /gene="guaA"
                     /locus_tag="PWS53_000272"
     CDS             complement(3053..4618)
                     /gene="guaA"
                     /locus_tag="PWS53_000272"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0870547.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(4677..5600)
                     /locus_tag="PWS53_000273"
     CDS             complement(4677..5600)
                     /locus_tag="PWS53_000273"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL0870548.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            5796..6401
                     /locus_tag="PWS53_000274"
     CDS             5796..6401
                     /locus_tag="PWS53_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0870549.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            6709..7725
                     /locus_tag="PWS53_000275"
     CDS             6709..7725
                     /locus_tag="PWS53_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0870550.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            7866..9167
                     /locus_tag="PWS53_000276"
     CDS             7866..9167
                     /locus_tag="PWS53_000276"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL0870551.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9213..9875
                     /gene="deoC"
                     /locus_tag="PWS53_000277"
     CDS             9213..9875
                     /gene="deoC"
                     /locus_tag="PWS53_000277"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL0870552.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            9896..10294
                     /locus_tag="PWS53_000278"
     CDS             9896..10294
                     /locus_tag="PWS53_000278"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL0870553.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            10360..11442
                     /locus_tag="PWS53_000279"
     CDS             10360..11442
                     /locus_tag="PWS53_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0870554.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            11654..13216
                     /locus_tag="PWS53_000280"
     CDS             11654..13216
                     /locus_tag="PWS53_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870555.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13209..14342
                     /locus_tag="PWS53_000281"
     CDS             13209..14342
                     /locus_tag="PWS53_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870556.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            14342..15298
                     /locus_tag="PWS53_000282"
     CDS             14342..15298
                     /locus_tag="PWS53_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870557.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            15397..16563
                     /gene="deoB"
                     /locus_tag="PWS53_000283"
     CDS             15397..16563
                     /gene="deoB"
                     /locus_tag="PWS53_000283"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL0870558.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            16587..17405
                     /locus_tag="PWS53_000284"
     CDS             16587..17405
                     /locus_tag="PWS53_000284"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0870559.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            17422..18129
                     /gene="deoD"
                     /locus_tag="PWS53_000285"
     CDS             17422..18129
                     /gene="deoD"
                     /locus_tag="PWS53_000285"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0870560.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(18427..19113)
                     /gene="gpmA"
                     /locus_tag="PWS53_000286"
     CDS             complement(18427..19113)
                     /gene="gpmA"
                     /locus_tag="PWS53_000286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0870561.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(19285..19866)
                     /locus_tag="PWS53_000287"
     CDS             complement(19285..19866)
                     /locus_tag="PWS53_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0870562.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(19999..20682)
                     /gene="rpiA"
                     /locus_tag="PWS53_000288"
     CDS             complement(19999..20682)
                     /gene="rpiA"
                     /locus_tag="PWS53_000288"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL0870563.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21001..21414
                     /gene="rpsL"
                     /locus_tag="PWS53_000289"
     CDS             21001..21414
                     /gene="rpsL"
                     /locus_tag="PWS53_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL0870564.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            21498..21968
                     /gene="rpsG"
                     /locus_tag="PWS53_000290"
     CDS             21498..21968
                     /gene="rpsG"
                     /locus_tag="PWS53_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL0870565.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22062..24149
                     /gene="fusA"
                     /locus_tag="PWS53_000291"
     CDS             22062..24149
                     /gene="fusA"
                     /locus_tag="PWS53_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0870566.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            24321..25508
                     /gene="tuf"
                     /locus_tag="PWS53_000292"
     CDS             24321..25508
                     /gene="tuf"
                     /locus_tag="PWS53_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0870567.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            25915..26223
                     /gene="rpsJ"
                     /locus_tag="PWS53_000293"
     CDS             25915..26223
                     /gene="rpsJ"
                     /locus_tag="PWS53_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL0870568.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            26254..26883
                     /gene="rplC"
                     /locus_tag="PWS53_000294"
     CDS             26254..26883
                     /gene="rplC"
                     /locus_tag="PWS53_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL0870569.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            26911..27534
                     /gene="rplD"
                     /locus_tag="PWS53_000295"
     CDS             26911..27534
                     /gene="rplD"
                     /locus_tag="PWS53_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL0870570.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            27534..27824
                     /gene="rplW"
                     /locus_tag="PWS53_000296"
     CDS             27534..27824
                     /gene="rplW"
                     /locus_tag="PWS53_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL0870571.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            27863..28696
                     /gene="rplB"
                     /locus_tag="PWS53_000297"
     CDS             27863..28696
                     /gene="rplB"
                     /locus_tag="PWS53_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL0870572.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            28739..29017
                     /gene="rpsS"
                     /locus_tag="PWS53_000298"
     CDS             28739..29017
                     /gene="rpsS"
                     /locus_tag="PWS53_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL0870573.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            29040..29387
                     /gene="rplV"
                     /locus_tag="PWS53_000299"
     CDS             29040..29387
                     /gene="rplV"
                     /locus_tag="PWS53_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL0870574.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            29401..30057
                     /gene="rpsC"
                     /locus_tag="PWS53_000300"
     CDS             29401..30057
                     /gene="rpsC"
                     /locus_tag="PWS53_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL0870575.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            30060..30494
                     /gene="rplP"
                     /locus_tag="PWS53_000301"
     CDS             30060..30494
                     /gene="rplP"
                     /locus_tag="PWS53_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL0870576.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            30484..30672
                     /gene="rpmC"
                     /locus_tag="PWS53_000302"
     CDS             30484..30672
                     /gene="rpmC"
                     /locus_tag="PWS53_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL0870577.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            30697..30963
                     /gene="rpsQ"
                     /locus_tag="PWS53_000303"
     CDS             30697..30963
                     /gene="rpsQ"
                     /locus_tag="PWS53_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL0870578.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            31021..31389
                     /gene="rplN"
                     /locus_tag="PWS53_000304"
     CDS             31021..31389
                     /gene="rplN"
                     /locus_tag="PWS53_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL0870579.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            31426..31734
                     /gene="rplX"
                     /locus_tag="PWS53_000305"
     CDS             31426..31734
                     /gene="rplX"
                     /locus_tag="PWS53_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL0870580.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            31762..32301
                     /gene="rplE"
                     /locus_tag="PWS53_000306"
     CDS             31762..32301
                     /gene="rplE"
                     /locus_tag="PWS53_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL0870581.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            32320..32505
                     /locus_tag="PWS53_000307"
     CDS             32320..32505
                     /locus_tag="PWS53_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL0870582.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            32541..32939
                     /gene="rpsH"
                     /locus_tag="PWS53_000308"
     CDS             32541..32939
                     /gene="rpsH"
                     /locus_tag="PWS53_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL0870583.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            32971..33507
                     /gene="rplF"
                     /locus_tag="PWS53_000309"
     CDS             32971..33507
                     /gene="rplF"
                     /locus_tag="PWS53_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL0870584.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            33668..34024
                     /gene="rplR"
                     /locus_tag="PWS53_000310"
     CDS             33668..34024
                     /gene="rplR"
                     /locus_tag="PWS53_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL0870585.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            34045..34545
                     /gene="rpsE"
                     /locus_tag="PWS53_000311"
     CDS             34045..34545
                     /gene="rpsE"
                     /locus_tag="PWS53_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL0870586.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            34560..34739
                     /gene="rpmD"
                     /locus_tag="PWS53_000312"
     CDS             34560..34739
                     /gene="rpmD"
                     /locus_tag="PWS53_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL0870587.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            34783..35223
                     /gene="rplO"
                     /locus_tag="PWS53_000313"
     CDS             34783..35223
                     /gene="rplO"
                     /locus_tag="PWS53_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL0870588.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35223..36518
                     /gene="secY"
                     /locus_tag="PWS53_000314"
     CDS             35223..36518
                     /gene="secY"
                     /locus_tag="PWS53_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL0870589.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            36580..37227
                     /locus_tag="PWS53_000315"
     CDS             36580..37227
                     /locus_tag="PWS53_000315"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL0870590.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            37418..37636
                     /gene="infA"
                     /locus_tag="PWS53_000316"
     CDS             37418..37636
                     /gene="infA"
                     /locus_tag="PWS53_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL0870591.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            37669..37785
                     /gene="rpmJ"
                     /locus_tag="PWS53_000317"
     CDS             37669..37785
                     /gene="rpmJ"
                     /locus_tag="PWS53_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL0870592.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            37803..38168
                     /gene="rpsM"
                     /locus_tag="PWS53_000318"
     CDS             37803..38168
                     /gene="rpsM"
                     /locus_tag="PWS53_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL0870593.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            38196..38585
                     /gene="rpsK"
                     /locus_tag="PWS53_000319"
     CDS             38196..38585
                     /gene="rpsK"
                     /locus_tag="PWS53_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL0870594.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            38665..39603
                     /locus_tag="PWS53_000320"
     CDS             38665..39603
                     /locus_tag="PWS53_000320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL0870595.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            39631..40011
                     /gene="rplQ"
                     /locus_tag="PWS53_000321"
     CDS             39631..40011
                     /gene="rplQ"
                     /locus_tag="PWS53_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL0870596.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            40283..41122
                     /locus_tag="PWS53_000322"
     CDS             40283..41122
                     /locus_tag="PWS53_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0870597.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            41098..41967
                     /locus_tag="PWS53_000323"
     CDS             41098..41967
                     /locus_tag="PWS53_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0870598.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            41957..42754
                     /locus_tag="PWS53_000324"
     CDS             41957..42754
                     /locus_tag="PWS53_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0870599.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            42957..43703
                     /gene="truA"
                     /locus_tag="PWS53_000325"
     CDS             42957..43703
                     /gene="truA"
                     /locus_tag="PWS53_000325"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0870600.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(44036..46705)
                     /locus_tag="PWS53_000326"
     CDS             complement(44036..46705)
                     /locus_tag="PWS53_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL0870601.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(46943..47131)
                     /locus_tag="PWS53_000327"
     CDS             complement(46943..47131)
                     /locus_tag="PWS53_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL0870602.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            47401..47763
                     /locus_tag="PWS53_000328"
     CDS             47401..47763
                     /locus_tag="PWS53_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0870603.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            47815..49497
                     /locus_tag="PWS53_000329"
     CDS             47815..49497
                     /locus_tag="PWS53_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL0870604.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            49614..51650
                     /gene="recG"
                     /locus_tag="PWS53_000330"
     CDS             49614..51650
                     /gene="recG"
                     /locus_tag="PWS53_000330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL0870605.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            51699..52700
                     /gene="plsX"
                     /locus_tag="PWS53_000331"
     CDS             51699..52700
                     /gene="plsX"
                     /locus_tag="PWS53_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL0870606.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            52822..53064
                     /gene="acpP"
                     /locus_tag="PWS53_000332"
     CDS             52822..53064
                     /gene="acpP"
                     /locus_tag="PWS53_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0870607.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        17220 a         9720 c        11486 g        14725 t
ORIGIN      
        1 tgaatccaat ttcaaataga aaggtcgctg attttattta cctatacaac tttataaaat
       61 ttagtcatac tcctagaaag tagataaaat tggcgactta atggagggaa aagtcgccat
      121 gagaaagata atctgtcatc tagttctctt aattaagttc ttatcaaaaa aacttatcca
      181 cctgtactgg atagatatga ccatcaatgt ggttttgcag attacataga tagaaaaatc
      241 aaagcatatg aacagagaaa acaatctgtt tatgtgcttt tttggtcact taataaatat
      301 cttttaggat ttctcttcat ttatgcaaca aatcccccgt tcgtgtcggg gtttagtatg
      361 aagggagata aagaagcaca tgccactccc tttcacgaca gaaagggggt gaaaagcatg
      421 aaaccatctt cttttcaaga aaccatagaa aatcagtttg actatatctg caaacaagtt
      481 attgaagatg aacgaaaaga ttacttcaaa catctagaca gactatcagc aaaagaagtc
      541 tcgttttctg atatagataa ctaccttgtc aactcttttt ctactgttga tacgtacgtc
      601 actgatttcc aactatttaa attatttgga ttaagaatca atgttgaaag tgatttatta
      661 agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca ttattttact tcattacttt
      721 atggatatga gtgatgtgga gattgcggag ttactaaatt tgaatcgttc gactgtttat
      781 cgtcatcgaa taagtggact agccatgatt aaagaattca tgaaggagtg taaagaatga
      841 aaacagctta tccaagggtt cccttccccc tcattgtaaa ggcgacagat ggcgacgtgg
      901 aagcaattaa tcagattgtt aaacattatc gagggtatac ctcaaaacgt tcattacgtc
      961 gcatgacaga cgaatatgga aattctcata tggttattga tgaaacccta cgtggcagaa
     1021 tggaaacgaa actcatctct aagattttat cgttcgaaat taaatagtcc cattttttcg
     1081 tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg aggtttgtta ttccatcaaa
     1141 gcatattgtg tgttcagtag gttttgatag gctaacaagc caaacgttct ttgacaacca
     1201 aataaaggca atcgaatacg tttcggtaag aaaagagctg atgaactaac acgccatgac
     1261 ctacaaaatt agtgagcgat tcatgttagt aattcgaaag taatctttag caggattgct
     1321 gccatgactt tcttatcgtg ataatgatac tcccgtatag ccatagtccg agcgttagaa
     1381 gcgtcacagg caatgggtac ggctacatga gaaccatgca gaagtgaaat tcctgtgagc
     1441 tttgctagag ctgttcgatt gctaagatat tcttctcttg tcagtgtatt attataaaac
     1501 tatttgatat aattataggt gaatattgga gttttcctag cctataattg ggaaaggagt
     1561 aataatgata acggtcacaa aaaaggattt agttgagcta ggatatggaa cttctttttc
     1621 agcagacatc ataaaaaaag caaaagaatt gatgattaca aaagggcata tctactatca
     1681 atctcgaaag ctagatagag tccctcgtga agctgttgaa gaaattctag gaattcggtt
     1741 ttcaaatgaa aacaggtctg agtagttttt gcacagtaag gagtaaaaac tatggttaaa
     1801 gacccaataa aaaaagcaaa gaatggaacc tattattttc gtgcaaatct aggttatgat
     1861 tctaaaggga aaaaaattca aaaatatcgt agtggattca ctactaaaaa agaagcaaga
     1921 gaagaatatt cgaaactgct attgatgaaa ccagaagaat taagtgagaa taaggataaa
     1981 atgacgttta aacattatat ctttgaaatt ttccttccat ggtataagac acaggtaaag
     2041 ctcagaacat atgaaaatcg cttaccaact attaagaagc atttctctta ttttgataaa
     2101 atggctgttt ccgatattga gcctattgat gtgcaaaatt ggcaactaaa attatcaaag
     2161 aagtgtaagt cttcttatgt aagagctgtt caaggactat tttcagttgc tatggatcga
     2221 gcaattgtcc ttgggataac caccacaaat ccatctaaga ttattggcaa tgtgaaaaag
     2281 caaaagtcta aaattgaatt ttggacaaaa gaagaatttg aaaaagtaat ctctctaatt
     2341 tacaaagaag attactatca acacttttta tttatctctc tgtggttttt atttatgact
     2401 ggaatgcgaa ttggtgaagc tactgccatt caatgggaag atattgattt tgactcagga
     2461 gtactaacca tcaacaaaac actctattac aagaatcaat cgaattatag atttgttgaa
     2521 ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata aatgcacatt aacttattta
     2581 tccgaatgga aggaagtaca acaaaatcta attcagacag actttgtaat gagctataac
     2641 ggtattccta cccaaaaaca tacactatca tatgctattg aacgctatgc taagctggct
     2701 ggtatccatc gaatcagaat ccatgggtta cggcattctc atgcttcctt gttaatcagt
     2761 atgggagaaa atccacttat cattaaagat cgtctaggac acgaagatat tgagacgact
     2821 ttaggaacgt atggacatct ttatcctaac agtaatttcg aggtagctaa caaattaaat
     2881 ggagccattt cctttaaaga agcaaaacaa aattatgata cttcaccaaa gaatcaattt
     2941 actgtagact acttgcgaaa caaagaaatg aaaaaagtgc aataaaagtg cagtaaaaag
     3001 ataagaagag ccagaaaccc ttggtataag aggatttcta gctctcctac aactattccc
     3061 actccactgt tgctggtggt ttagaagtga tgtcatacac gatacggttg acgtgggcta
     3121 cttcgtttac gatacgtaca gagatttttt gcaagacttc ccatgggata cgggcaaagt
     3181 cagcggtcat tccgtcgatt gaagttacag cacggatacc gactgtgtag tcgtaagtac
     3241 gtccgtcacc cataacaccg acagagcgga tccctggaag aacagtgaaa tattgccaga
     3301 tatcacggtc aagtccagca gctgcgattt cttcacgcaa gatagcatca gattcgcgta
     3361 cgatttctag cttgtcttca gtgatttcgc caagaacacg aataccaaga ccaggaccag
     3421 ggaatggctg acgccaaacg attgaatcag gcatgcctaa ctgagtccct aattcacgga
     3481 cttcgtcttt gaataatgtg tttaatggtt cgatcaattc aaattgcata tcttctggca
     3541 gtccacctac attatggtga gatttgatcg tctgtgctgt ttctgtacca gattcgataa
     3601 cgtcagtata caacgttcct tgtgctagga aagagacgcc ttcttcacca gcaagttttg
     3661 ttgcttcgtc atcaaaaaca tatacgaatt cattcccaat gattttacgt ttttgttctg
     3721 ggtcagatac acctttcaac ttgtctaaga agcgatctct tgcatcgact ttgatgatgt
     3781 tcaagccgaa ttttccgcct agactttcca ttacttgttc cgcttcgcct ttgcgtagca
     3841 gaccgtggtc aacaaagata gatgtcaatt ggtcaccgat cgctttttgt aaaagaaccc
     3901 caacgacact agaatcaacg ccacctgaaa gaccaagcaa gacttttttg tcaccaactt
     3961 gttcacggat tttttctact tgcatatcga taaagttatc cattgtccag ttaccttcac
     4021 agtgacaaac atcaaaggca aagtggcgca gcaattcgat tccgtattcg gaatgacgta
     4081 cttctgcatg gaattggata ccataaaaat tacgtgttgt gtcttggata gaagcaatcg
     4141 gacaatcttt gcttgttccg actgtttcaa agccttcagg aactttagtt accaagtcac
     4201 catggctcat ccatactgtt tgttgttttg gtgtttcttt aaacaagaca gcttgatcgc
     4261 ttgttacttc taacaaggct ttcccatatt cgcggttttt agcaggctct acggaaccgc
     4321 caagtttgta cgtgatcaat tgcataccat agcaaatccc taatacagga atacctaaat
     4381 tgaagatctc aggatcgatc ccaaatgcat tctcatcata tacactgttt ggtccgccag
     4441 aaagaatgat tccctttgga ttcatcgctt ttacttcttc tgcagtaatg cggtggctca
     4501 ataattcgga aaagactcca agatcacgaa tacgtcttgt gatcaattgg ttatactgac
     4561 taccgtagtc taatacgata atcttttcaa cgttcgtcat atcggcaacg tttgtcaaat
     4621 ttattacccc tatcccttta aattttatct caaaatagtc aaactatttt aaaatgctaa
     4681 tattttcgca tgaaaatcga atcgatgata tgattttcag ttttatggag aattatatcg
     4741 gctcttcctc gtgtaggcaa gatatattcc tgtaaatttt tcaaattcac atttttccat
     4801 acatcttttg ccattttaaa ggcttcttca cgatctccga tagcaaaacg ataataataa
     4861 ttctctggat tttggaaagc cgtatccagt aacgcaccaa aacgatccaa ataccatttt
     4921 tcgatcagca cgggatcggc atcgacatag acagaaaaat caaaaaagtc gctgacgtag
     4981 atttgctgat tcgcaggtaa ttgaagcgta ttgatcccct caacgatcaa gatatcaggc
     5041 tgctggatca attcgtactt gtctggaatg atatcataca catcatgtga gtaaacaggt
     5101 acttttatct cttcctgacc agattttaca cggttcaaga aatcgatcag catctccata
     5161 tcataacttt caggaaatcc tttacggtcc atgattccac gctcttttaa tatacgattt
     5221 ggataaagaa atccgtcagt cgtgatcagc tgaacgtttc ttcgtttgaa ggtacgagac
     5281 aaaatcgtct gcagcagtct ggctgttgta ctttttccaa cggctacact tccggcgatc
     5341 ccgataataa aaggcggtac ttctacgtat ttatgaagaa ataaaccttt actcaagaac
     5401 aaagattcaa attctttcat ataaagataa atcagatgag caagaggtac atagatatct
     5461 cgaacatcct gcatggaaat gcggtcattc aaacttttga tattatctag ttcatcttgt
     5521 gtcaaaggtg cttgcccatt acgataaaat tctcgccatt cttctcttgc cacacggtag
     5581 tagttcattg gatcattcat attagcctcc tgcgttctcc catcaatgga actattttaa
     5641 cacaaaataa acgcaaaccc caccacttaa gagagattgc cgatcttttt gtataatctt
     5701 tataatagaa agaaacggag tcttattctt gacttgcgca cggatacata aaaggataga
     5761 ctgttgtcat gaaagaaatg agaaggatgg aagacatgac aaatcattac tattctgaac
     5821 gcccagatac ggcgcacgat tttgaacaat gggcttttga gttaaaaggc agaacctttc
     5881 aatttgtaac agattctggt gtattttcac gtgaaacagt ggattatggt tcacgtgtcc
     5941 tgattgatgc tttcgattgg gaagagttac ctgatgaagg aaagatctta gatgtgggat
     6001 gcgggtatgg accgattggg ttagctttgg catttgcgac acagcggttt gttgaaatgg
     6061 tcgacattaa tgaacgagcg gtaagtctag cgcaaggaaa cgcaaagcgt aaccaaatcg
     6121 aacatgttga tatccatcaa tcgaatatct atgaagcagt tcatgaagaa acctatgcag
     6181 ctatcgtaag caatccgccg atccgtgctg gaaaaaaagt tgtacatgaa attttgacag
     6241 gcgcatatcc tcggttgaag aagggcggta cactgacaat tgtgatccag aagaaacaag
     6301 gagcgccgag tgcgcaaaag aaaatggaag aaacatttgg taatgcagaa atcgtcacaa
     6361 aagataaagg atattatatc atcaaaagtg tgaaagaata actagctgaa aaatagtgga
     6421 gcgacgaaag agtggaactg ggcgtgcatg tacgggtacg tatcagttct ttttttgttg
     6481 agaagttttt aaaaacaaag aactattttt tcttcaaaaa gaaatagtag gaacagagtc
     6541 gttgtttttt acgataaaat aaataagata cagaacttgt atagaaggct gccaagtcgg
     6601 tagataaagg aggtttttat caaaaaacat actttccttt tttctctatt cttttcttgg
     6661 gggttttggt acactgatag ggaatttaga tagaagggag ccaataggat gaagctgacc
     6721 atcagagata tcgcagaaat ggcaggcgta tcagtaacaa ctgtctcaca gattttgaat
     6781 aacaaaggaa gcagatttag tgataagaca agaaaaaaag tgttgtcgat cgtgaatgaa
     6841 catcattata agcccgatta tttcgcttca aatgtgatca atcgacattc aaagaccatc
     6901 ggaatgatcg tccctgatgt cactgatttc ttcttctcca aactgatcga aggtgtagag
     6961 agctatttga atcctttggg ctatgtgatc atgttatgca attctcgtca tagccaagaa
     7021 aatgagatca agtatttaca agagctttcc caccgctcag tggacggaat cttgctggcc
     7081 acaccaaata ttttacctga gaaatatgca ttagacagcg gtttttatca aaaaatgcca
     7141 atcattttaa tcgatcgcgg attgaatcgt cgggataacg gacggcttat cgtaaaggaa
     7201 tatgagggcg cctatcaagc tgtttcgctg tttattgaaa atggccatac gaagatcggc
     7261 atgctgaagg aaaccactgg atactatcaa ttagaagaac gtttcaacgg atatcgtcat
     7321 gcgctgaaag atcatggcct gccttttaat gggaaatttg ttgaacatgg agagttgact
     7381 gtccaaggtg gatatactgc atcaaaaaaa ttactcaaac ataaagatat cacggctatt
     7441 ttttgcggga atgatgccat ggcgatcgga tgctatcagg cgatcgatga attgggcaaa
     7501 aaaatcccag aagatatctc agtcatagga ttcgatggat taaagctgtc agaatacatg
     7561 attccgcaat tgacgacagt gcagcagcca agttttgata ttggatttta tgctgcacgt
     7621 ttcctgatcg atacgatcga atttcctcaa agaaaagttc cgaacaaagt gttcgaaaca
     7681 aaattaatta ttcgtgagag cgtaaaagta ttgacaaagg gctaactcgg cggtatattg
     7741 cttatgtaag catttacaaa ggctagttag tgcttttttt atttttagta aaccagttta
     7801 ctaaagtggt ttacctttcc taaaaataat gtataatacc gaatgaagac aaggaggatc
     7861 aggcaatgcg aatggtggat ctgattgaaa aaaagaggga tggaaacgag ctgtcgaaag
     7921 aggaaatcga gtacatcgtc acgaactata cgaatggaaa gattccagat tatcaagtca
     7981 gtgcattatt gatggcaatt ttttatcaag acatgacaaa tgaagaaatc acgaacttaa
     8041 cattggcgat tgctaattct ggtgatgtca tcgatttgag ttctcttgaa ggaataaagg
     8101 tagataagca ttcgacaggt ggcgttggtg acacgaccac attgatttta gcccccctag
     8161 tagctagtgt cggagtgaca gtggctaaaa tgtctggacg cggtctaggt tacacaggtg
     8221 gtacactaga caagctagaa gcaattcccg gatttcagat cgagctttcg gatgaagaat
     8281 ttgtacgcat tgttaatgaa agtaaagtag cggtaattgg tcaatcagga aacttggcac
     8341 cggcggacaa aaaattgtat gccttgcgtg acgtcacagc gaccgttgat tctcttccgc
     8401 tgattgccag ttcgattatg agtaaaaaaa tcgcggcagg agcagatgca atcgttttgg
     8461 atgtgaccac tggagacggt gctttcatga agaacatcga agatgcaaga cgtctggcaa
     8521 agaccatgac gagcattggg aaactggcta accgagaaac agtggcggtt atctcagata
     8581 tgtcagaacc tcttggcgaa gctatcggaa atagtctaga agttgtcgag gcaatcgaaa
     8641 cattacaagg aaatggtcca gaagatctag tagaaatgtg ctacgcctta ggaagccaaa
     8701 tggttgttct tgctggcaaa gctaagacaa ccgatgaagc aagaacgttg cttcaagaag
     8761 ctctggaatc aggtaaagcg ttagcaaaat tcaaagaaat gatccaaaat caagggggag
     8821 acccagcgat cgtggaacat ccagaacgaa ttttgactgc acgctacaca atggagcttc
     8881 ctgctaaaca aagcggagtt gtgtcaaaaa tcgtggcaaa tgaattagga attgctgcaa
     8941 tgatgctagg tgctgggcgt aaaaccaaag aagaagatat tgatcacgct gttggattga
     9001 aactgcataa aaaaattggt gacactgtga caaaaggtga gtcactattg acgatctata
     9061 gtaacgacga agaaatcact tctgtcattg aattattata taaaaatatt gaaattggtg
     9121 aatcggcaat gaagccaacg ttgattcatg acatcatcac agaataaagg aaaaaagcgg
     9181 actttaccgt tttaaagaaa aggggaaaag aaatggaact aaaccgaatg atcgaccata
     9241 ctattttgaa agcagacgca tctaaagaag ctgtgatgca aattatcgaa gaagcaaaaa
     9301 aataccattt ttattctgtc tgcatcaatc cagcatgggt tgcgctagcg aaagaacaat
     9361 tacaaggtga gccagtagcc gtatgtacag taatcgggtt tcctctagga gcaaacacac
     9421 ccgagacaaa agcatttgaa acaacagatg ccatcaataa cggtgcagat gaagtcgata
     9481 tggtcatcaa tatcggtgct ttgaaatcaa aggatgatca acaagtccaa aaagatatcg
     9541 aagcagttgt tgaagcagca aaagatcgtg cgcttgtcaa agttattatt gaaacatcgt
     9601 tattagatag agaagaaatc atccgagctt gcgaaatagc aaaagctgca ggcgctgatt
     9661 ttgtcaaaac atcaactggt ttctcaacag gcggagcaaa ggtagaggat gtccgtttga
     9721 tgcgtgaaac tgtaggacca gaaatgggtg tcaaagcttc cggcggtatc cataacgaag
     9781 aagaagcaat ggcaatgatc gaagctggtg ctacaagaat cggaacaagc gcaggagtag
     9841 caatcgtatc tggttcaact ggtgaaggct attaattttt tcaaaggaga agctcatggc
     9901 agaactcaaa caagaatgga tcaacacagc tattgaagcg ttagataaag cctatgttcc
     9961 ttattcccat tttccggtag gcgcttgtct cgtaacggaa tcaggcaaga tttatcaagg
    10021 catcaacatt gagaatgcat catttggatt aacgaattgt gccgagcgca cagctttttt
    10081 caaagcagta tctgaaggag aaaaaacatt tacgcatcta gtcgtagcgg ggcacacccc
    10141 cgatcctatc tctccttgtg gtgcatgtcg acaagtaatg gcagaattct gttcccctga
    10201 tatgccggtt acgctagtag gagacaatgg tgtgacgaag gcaacgaccg ttcgtgcact
    10261 gctgccttat gcatttactg aaaaagatct ataactcatg ttcattcagt cagctgactg
    10321 ttgaaaaata aaacaattat tttaggaggc tttacagaaa tgaaaaaagc aaaattattt
    10381 ggtttaggtg ctgtcgcact agcagcaggg ttatttttag gtgcatgcgg caacaacggc
    10441 tctactgatt catccggtgg caaaagctca tcagacacaa caactgcagc attgatcaca
    10501 gacacaggtg gtgtagacga ccgttcattc aaccagtcag cttgggaagg actagaaaaa
    10561 tgggggaaag atcaaggact ttcacgagga aatgatggct tccaatattt ccaatcttca
    10621 aatgaatcag attacatccc aaatattgac caagcattga atgctggatt caaaacgatt
    10681 tttggtattg gttataaact aaaaccagcg atccaagaac aagcaacaaa caatacaggt
    10741 acgaactttg ttattatcga tgatgtcatc gatggattag ataacgttgt atctgcaact
    10801 ttcaaagaca acgaagcttc ttatcttgca ggtgttgcag cagcttatac gactgaaaca
    10861 aacgtagtcg gctttatcgg cggtgtaaaa ggtgaagtaa tcgatcgttt tgatgctgga
    10921 ttcaaagccg gtgttgatgc aggtgcgaaa gaattaggaa aagaaatcaa agtattgaat
    10981 caatatgctg gtgacttctc tgctccagat aaaggacgtt caattgctca aggtatgtat
    11041 gcacaaaacg cagatatcat cttccatgct tcaggcggta caggtaacgg tgtattccaa
    11101 gaagcaaaat cattgaacga atcaggcgac aaaaaagttt gggttatcgg tgttgaccgc
    11161 gaccaatcag acgaaggcga atacacatta aacggcgaaa agaaaaactt cacattgaca
    11221 tctacactga aagcagttgg aacagtagta gaagatttgg cacaaaaatc agcggacggt
    11281 aaattccctg gcggcgaaca cactgtttat ggattgaaag aagacggtgt tggtctaaca
    11341 gaaggacaac tttctgacga agcgaaaaaa gcagttgatg aagcaaaaga aaaaatcatt
    11401 tcaggtgacg taaaagttcc agaaactcct gaagaaaact aaaaccattt ctatagcctt
    11461 gttagacaca tgtttgacaa ggctatggtt tgattagaac aagaattgtt catttatcca
    11521 agctgccttg tgacagtttg gataagggaa caactccctg aagtaatgtt tgtttcaaaa
    11581 gaagaacgct cactaagcgt gaatcttagt caatcgttgc cctagataga aatcgttagg
    11641 atgtgaaaaa aaggtggttc aagaaaatat tgtcatagaa atgcagaata ttaccaaaga
    11701 atttggaaca ttcaaggcaa atgacaacat caatttacaa gtaaaggcag gagaaattca
    11761 tgcgttgctt ggagaaaacg gtgctggcaa atctacattg atgaacgtgc tttccggatt
    11821 attagagccg acatcaggga aaattttgat gcgtgggaaa gaagtaaaga tcacaagtcc
    11881 gaccaaagcc aaccaattag ggattgggat ggtccatcag cactttatgc ttgttgatgc
    11941 ctttactgta acagaaaaca tcgtgttggg aagcgagcct agtcgcgcag ggatgcttga
    12001 ccataaaaaa gcacgaaaag agatccaaaa agtttctgaa cagtatggat tgtcagtcaa
    12061 cccagatgct tacgttcgtg atatttcagt tggaatggaa caacgggtag aaattttaaa
    12121 aacactttac cgaggagcag atgtactgat ttttgatgag ccgacagctg tattgacccc
    12181 tcaagaaatt gatgaattaa tcgtgatcat gaaggaatta gtcaaagaag gcaagtcgat
    12241 cattttgatt acgcataagt tagatgaaat caaagcagta gctgaccgct gtacagttat
    12301 ccgccgtgga aaaggaatcg gtacagtcaa cgttaaagac gtaacctcac aacaattagc
    12361 cgatatgatg gttggaagaa cggtttcatt caaaacgatg aaaaaagaag cgaagcctca
    12421 agaagtcgtt ttgtctattg aaaatctagt ggtgaaagaa aatcgtggat tagaagccgt
    12481 gaaaaacttg aacttagagg ttcgtgctgg cgaagtactt ggtatcgctg gaatcgatgg
    12541 aaacgggcag tcggagttga tccaagcttt gactggtttg cgaaaggcag aaagcggaca
    12601 tatcaagcta aaaggggaag acatcactaa taaaaaaccc cgaaaaatca ctgaacatgg
    12661 tgtaggacac gtgccagaag accgtcataa atacgggtta gtcctagata tgacattatc
    12721 tgaaaatatc gcactacaaa cctatcatca aaaaccttac agtaaaaacg gcatgctgaa
    12781 ttattcagta atgaatgaac atgccagaga attgatcgaa gaatatgatg ttcgaacaac
    12841 gaatgaactt gttcccgcaa aagctttatc aggcggaaat cagcaaaaag caattatcgc
    12901 tcgtgaagtc gaccgagatc ctgatctgtt gattgttgca aatccaactc gtggactgga
    12961 tgtaggtgcg atcgaattta ttcataaacg tctgatcgaa caaagggaca aatacaaagc
    13021 agtgttactg atcagtttcg aattagaaga aattttaaat gtttcggatc gtattgctgt
    13081 tatccatgaa ggagaaattg tcggtatcgt tgatccgaaa gaaacatctg aaaatgaatt
    13141 aggtctattg atggcgggct ataccttaga agaagcacga aaagagttag aaaagggggc
    13201 tggcgcacat gaatgatcgc agcgaaaaat tccgtaattt attagtgcct gtactttcag
    13261 ttgttttagg attgatctta ggcgcgatat tgatggctgc ttttggtttt cagcccatcc
    13321 aaggttattc ttctatgcta gatgcagccc ttggttcgca aagagggata ggtgaaacgt
    13381 taaggcaagc aacgccgttg atttttacag cattaggttt ttctgtagct aactcagctg
    13441 ggtttttcaa tattgggctt tctggacaag cactttgcgg gtggatcgtc agtgtctggg
    13501 tggcacttgc gtttccagat ctgccaaaag ttgttttgct tccgttatgc gtgatcttag
    13561 gtgccttagc tggtgcgatg gcagcggctg ttccaggttt acttcgagca ttttttggaa
    13621 caagtgaagt gatcgtaaca atcatgatga actatatctt attatacgta agcacatttg
    13681 ctttacatga agtgatgcct gaaagctatc gctcaagttt ggactcttcc aatatgatca
    13741 gtcaaaatgc ctcactgcgg ttaccatggt taacacaagc ttttggcggc tctcgcgtaa
    13801 actcaggatt atttttagca atcatcgcgc ttattttcgt ttggctgctg atgaagaaaa
    13861 cgactttagg atttgaaatc cgttctgttg gattgaatcc ttttgcttct gagtatgcag
    13921 gaatgagtag taaacgtacg atcgttttgt cgatggtcat ttctggcgct ttggcagggc
    13981 ttggcggtgt ggttgaagga ttaggaacgt atcaaaactt ctttgttcaa acgacttcgc
    14041 tatctattgg atttgacgga atggctgttt ccttgcttgg ttctggatca gcaatcggta
    14101 ttcttttatc agcgatcttg ttcagtattt tgaagatcgg tggacttggc atgcagacag
    14161 gagcaggtgt tccgtttgag atcgtcaatg tatcgattgc tttgattatc ttctttgtcg
    14221 ggatcaactt cctgattcgt ttcgtcatgg ctaaagtctt ccaagggaaa aaacaagaag
    14281 agatcgttgc gacaatagaa tcgaaaccaa cgaatcaaaa ccatgaaggg ggcgaactat
    14341 aatgtcgact atcgaattaa ttgccttgat ccttacttct acactggttt attcaacacc
    14401 gctaattcta acgtcactag gcggaacttt ttctgaacga agcgggatcg taaacgtagg
    14461 gctagaggga atcatggtca tgggtgcttt tagttccatc gttttcaact tgacgatgag
    14521 tccatcactt ggttctatga caccatggat cgctatttta gtgggtggcg tcgtaggtat
    14581 cctattttct ctgattcacg cagtagcaac agtcagtttg cgtgctgacc atattatcag
    14641 cgggacagta attaatctga tggcacctgc tttaggtgtt ttcttgatca aagcatggta
    14701 tggtaaaggt caaactgata acatcacgca gaatctgggg tatttttctt tcccaggatt
    14761 agcagatatt ccggtcattg gaccaatttt ctttaaaaat acattcttac ctgcttattg
    14821 tgctattttg attgctgtcc tagcttgggt gatcatcttc aagacgaaat tcggcttgcg
    14881 cctccgttca gttggtgaaa atcctcaagc tgccgacaca ttaggtatca atgtatatgc
    14941 tatgcgttat tcaggcgtac tgatttctgg cttgttaggc ggtatgggcg gtgctgtttt
    15001 tgctcagtcg atttccggta acttttcagc tggtacgatc gttggacaag ggttcatctc
    15061 catggctgct atgatctttg gtaagtggaa tcctttaggg gcaatgggtg cctcattgtt
    15121 ctttggtttt gctcaaagct tgagtatcat tggcgcacaa ttaccagtta tttcttcgat
    15181 tccgccagta ttgctgcaaa ttgctccata tttgctgaca atcattgtgc tggttgtttt
    15241 cttagggaaa gcttctggtc caaaagcaaa cgggaagaac tatatcaaat caaaataatc
    15301 ttttagcgaa taaaaagggt gggttacggg aagtgttttt cgtctcgccc tttttgtttt
    15361 aaaggcaata aaaataaaaa gaaagaagga ataatcatgt ttaaacgcgt acatctgatc
    15421 gtcatggatt cagtaggaat tggagaagca ccagatgccg ataagttcgg tgatgttggt
    15481 tctgatacat taggacacat tgcgaaagaa gcaggattga acgttccaca tttagagcag
    15541 cttggtttag gaacgatccg tcctcttgaa ggtgtggaat cagtgaaaga tcatcaaggc
    15601 tatgcgacga aattagaaga agtttctgta gggaaagata cgatgacagg ccactgggaa
    15661 atcatgggat tgaatatcaa aactccattt cgtgtgtttc ctaatggatt ccctgatgat
    15721 ctgatcaaac aaatcgaaga attttctggc cgtaaagtcg tatgtaacaa accttatagc
    15781 ggaacagctg ttattgacga ttacggtgaa caccaaatga aaacaggcga tttaattgta
    15841 tatacttctg ccgatcctgt ccttcagatc gcggcacatg aagaaatcat tcctttagaa
    15901 gaattatatc ggatttgcca attcgttcgt gatataacaa aagatgatcc gtatatgatt
    15961 ggccgaatca ttgcacgtcc gtacgtgggt gaaccaggag acttcacacg aacaagcaat
    16021 cgccatgatt atgcgttgaa tccattcgga cacacagtcc ttgattcttt gaaagaagca
    16081 ggaaaggacg tgattgctgt tggaaagatc aacgatattt tcaacggaca aggaatcact
    16141 gaatctgtcc gcacgaaaag caatatggat ggcgttgacc agctgctgaa agttatgaaa
    16201 aacgattttt caggaatcag ctttaccaat ttagtagatt ttgatgcatt atacggtcat
    16261 cgtcgggatg tagtcggcta tgcgcatgcg attgaagaat ttgacttacg aattccagaa
    16321 ttgcttgaag cgatggaaga agacgacttg ctaatgatta cagccgatca cggaaacgat
    16381 ccgacgttta ctggtaccga tcatacgcga gaatatgtac cgttattggt ttacagtaaa
    16441 aaaatgaaag aacaaggggc attgcctcaa ggatattatt cagatatttc agcaacgatt
    16501 gctgagaact ttagtgtttc ttcgactgaa aatggcgaaa gcttcttgaa attattaaaa
    16561 taatcttttt aaataggaga aagcaaatga ccaagttaag cgagatgtta caagaaacga
    16621 cccagtttat ccttgataaa ggagtaaaag aaattgattt tggtttgatt ctaggttcag
    16681 gattaggtga attagcagat gaaatcgaag atgctgtaat gatttcttat gatgagatcc
    16741 cgttctttcc tgtttcgaca gtagttgggc atgcaggaaa gctagtttat gggtctctgg
    16801 caggcaagaa agtgttagcc atgcaaggga gattccacta ctatgaaggt cattccatgc
    16861 aaacagtgac ctatccagta cgtgtgatgg ccggactagg ggcccattca gtcattgtga
    16921 ctaatgcatg tggtggtgtc aacgaatcat ttgtaccagg agatttgatg ctgattaccg
    16981 atcatatcaa ttttactggt gaaaatcctt tgatcggtcc gaatgaagat gcaatgggcc
    17041 ctagatttcc agatatgagt caagcttata cgcctgacta tcaagcaaca gcaaaagcag
    17101 cagcagaaaa actaggtttg gatcttaaag aaggcgtata tatgggctat tctggaccaa
    17161 cttatgaaac gcctgcggag atccgaatgt ctcggacaat aggagccgat gcagtaggga
    17221 tgtcgactgt tccagaagtc atcgtagcag ctcacagcgg attaaaagtg ttaggaatct
    17281 cttgtattac caacttagca gcaggtatgc aagcgaatct gagccatgaa gaagttgtag
    17341 aaacaactca gagagtcaaa caatctttta aagcattggt taaagaaaca ttgacattat
    17401 tgtaatagga ggaacaataa aatgagtgtg catattgaag cgaaagaagg cgagatcgcc
    17461 gataagattt tacttccggg agatcctttg cgagctaaat atattgcgga gacttttttg
    17521 gaagatccag tttgttacaa tcaggtacgc ggtatgttag gctttaccgg caaatataaa
    17581 ggagaacgta tttctgttca aggaacagga atgggaatgc catcagccac catctatgcc
    17641 catgaattga tccagtcgta tggtgtaaag aaattgattc gagtaggtac atgcggtgca
    17701 ttgtcaaaag acgtccatgt tcgtgattta gtactagcac aaggtgcagc gaccagttct
    17761 tcgatgatcg aaaaaaattt ccaagccttt cattttcctc caatcagtga tttcaatcta
    17821 ttactgaaag catatgagat agctaaagaa aagggctata cggttcatgt aggaaatgtt
    17881 ctttcggaag attctttcta caaagatgat ttgacagaga cgttccaatt agctgaactt
    17941 ggcgttttag gagtggaaat ggaagcagcg gctctttatt acttaggcgc gaaataccat
    18001 gtgcagacat taagtttgat gaccgttagc gaccatttga tcaccggtga agaaacaaca
    18061 gctgcagaac gtcaatctac gttcaatgaa atgatcgaag taggattgga aacagcgata
    18121 gcagaataag aaggaaatga aaaagctgcc ctgcattaag taataagaac atccaagcaa
    18181 aaatagctcc tcatgttttt gacttggctg gacttattat tgcaaatgta agcttttgaa
    18241 caccgtttac caagaggttg tgaatctgcc gtttaactct gagtcacaag gaacaatttc
    18301 aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt gaccgagaag ttggcaggag
    18361 aaaaccgttt atacgaaaaa agggactgtg acaaatcgtg tcacagtccc ttttttatag
    18421 tgttttttat aggtagtatt ttttagcaac tgttaagtca tcatttaatt cataaacaag
    18481 tggttgacct gttggaattt caagatccat gatgtcttcg tcagagatac cttcgatatg
    18541 tttagctagg gcgcgtaatg agttaccatg tgcagctact aaaacagttt tgttgtctag
    18601 taatgctgga gcaatttcat cttgccagaa tggcaaagcg cgttctaatg tcactttcaa
    18661 gttttctcca ccaggaatat cgcgtttgtc taacattgcg taacgacggt catttgctgc
    18721 agaaccttcg tcagtttctt ccattaatgg aggaagagta tcgtaagaac gacgccagat
    18781 gtgcacttgt tcgtcgccgt atttttcagc tgtttctttt ttgttcaaac cttgtaattt
    18841 accatagtga cgttcgttta agcgccatga tttgatttgt ggtacccaaa gttgatctga
    18901 atattctaag atcaagttac atgttttgat tgcacgagtt aaaacagaag tgtaagcaac
    18961 atcaaattcg attccagctt ctttgatttt gcgtccgcct tcttttgctt cttcgattcc
    19021 ttctggtgct agatttacat cagcccaacc agtaaattgg ttcaatgcat tccattcact
    19081 aagaccgtga cgagaaaaaa ctaattttgg catgtttagc tctcctttta agataggttt
    19141 atatttctga aattttcagt tctgttttat tgtacaattt tttggaagag atttccatcg
    19201 aaaaattaga taagattcta tgagaaaaca agtagagaat ataaaaaaac gaacagtgcc
    19261 cacgcaactg ttcgttttta ggtattagat gcataattgc tcgatttctt tttcaggtaa
    19321 attgatatat ttataacatg ttccaactga aacaccgcat tttgaagaaa taaattggat
    19381 cgtttctttt ttttcataat agagacgtcg gatttttttg atcgtttttg catcgatttt
    19441 tggtcttccg ccgatttttc cttttttacg agcttcatct aatccaacta atgtacgttc
    19501 tttgatcaat gcgcactcca tgtttgctag gttttccatt aattggaaat agatttttcc
    19561 catttcgcgc gtatcgattc cttcatcaag actaatcaag tgcagcccgc gatctttgaa
    19621 taattgcgtg agttctgtta attgccgcgt agatttgcct aatcgatgaa gttggcaaat
    19681 gactagtgca tcgccttgtt gcatgtcagc gatcactgac tccaaatgaa cagcagtaga
    19741 atcattttgt tccacttcat atgtctcatg gaaaatttct tgacaaccaa agtcagataa
    19801 ttgatcaacc tgagttgtta aatcaacgtc atttatggtt gtgcgtgcat aaccaataat
    19861 tttcatggtg attcctttct agcagtgatt ttctaactgg atataacagt aaaaatgtag
    19921 cataaagtac gtcaaaaaga tagttttttc gcaaattttc cgctgtcttt ttttattgga
    19981 tgatcaagca gtttttcttt atttacgacg gattgcgtca agaatctcaa cgcctttttc
    20041 tgagccgaca actactttag aaaccatgtt taggaaaagc ccatgttcta ctaccccaac
    20101 aagttcatct aaataggtag ctaagctttc tggctgttcg atgacttcta gatgcagatc
    20161 aatgatgtaa tgaccaccat ctgtggttaa gacttctccg ttttcgtctg tacgcaagac
    20221 gggttggaat cttttttctt caaaaatatg aagtagctgt tgggagccat aagggatcac
    20281 ttcaactggg agtgggaact tcccaagttt atcgactaat ttagaagaat caacgatcca
    20341 aatcgtttct tttgagtacg tagcgactat tttctcgaaa agcaaagcag cgccgccgcc
    20401 tttgatccct tgaaaatcag cactgatttc atcagcaccg tctattgtca aatcgactac
    20461 ggggacttca tcaatactct ttaacggtat tcctaatttt tccgcttgtt cttttgtcgc
    20521 atttgaagta gtaactcctg tgatacgtaa accttcttca cgcatacggc gaccaatctc
    20581 ttctaccata taataggcgg ttgaaccggt cccgagacct acgatcatcc cgtcctttac
    20641 gaatttcgcg gcttcaatgc cagctaattg ttttaaattc atctttgcgt ccaccttttt
    20701 ttagtttgag catcgtgctt tcctcactat tttagcgtga tttacggtaa aaagatacca
    20761 ttctttttta ttccaataaa gctttttcaa aaaaaattgc atctcttgtt gacatgtttt
    20821 ttcattcgtg atattctaac aaaggtgctt tatctacagg tttctgtgaa gaaacgtgct
    20881 gactgtttat caaagcatat ttgataagga tgcagacatt gcatttattt tttatcacaa
    20941 aaaaagaacc acctggatgt gtggaactag acgcaaatcg tgggaaggag gaaacaagga
    21001 atgcctacaa ttaaccaatt agtacgtaaa cctcgtaaat caaaggtgga aaaatctaat
    21061 tcacctgctt tgaacaaagg atataacagc tttaagaaaa ctcaaactaa cgtaaactca
    21121 cctcaaaaac gtggggtagc aactcgtgtg ggtacaatga cacctaaaaa accgaactca
    21181 gctttacgta aatatgcacg tgttcgtttg tcaaacttaa ttgaagttac tgcttatatc
    21241 ccagggattg gtcataactt acaagaacac agtgttgtat tattacgcgg tggtcgtgta
    21301 aaagacttac caggggtacg ttaccatatc gttcgcggtg ctttagatac tgctggtgtt
    21361 aacgaccgta aacaaagccg ttctaaatac ggaactaaaa aacctaaagc ttaattttaa
    21421 ttattaaaca aatgaaactt gcttctacta tatagaagta attactatga acaatatttc
    21481 ggaaggagga gttacggatg ccacgtaaag gtcctgttgc aaaacgtgat gttttaccag
    21541 atcctattta taactcaaaa ttagtaactc gcttgatcaa ccgtgtaatg gttgacggaa
    21601 aacgcgggat tgcagctaac attatctata atgcctttga cttgatcaaa gaatctacag
    21661 gtaacgatcc attagaagtg tttgaacaag caatgaaaaa cgttcaacct gttcttgaag
    21721 ttaaagcacg tcgtgttggg ggttcaaact accaagtacc agttgaagtt cgccctgaac
    21781 gccgcgttac tctaggtctt cgttgggtag taaactacgc tcgcttgcgc ggtgaacaca
    21841 caatggaaga acgcttagca aaagaaatca tggatgctgc aaacaacaca ggtgcttctg
    21901 ttaaaaaacg tgaagacact cataaaatgg ctgatgccaa ccgtgcgttt gctcactatc
    21961 gctggtaaga ttctctctgt ttggtcggct tttataagtc gacctcagat agtgtcctta
    22021 tttttagcaa ctagatagta aagagaggag aaacaaggag aatgaccaga gaattttctt
    22081 tacaaaacac tcgtaatatc ggtatcatgg cccacatcga tgctggtaaa acgactgcta
    22141 ctgaacgtat cttgtactat actggtaaaa tccataaaat tggtgaaact catgaaggag
    22201 cttcacaaat ggactggatg gaacaagaac aagaacgtgg tattacgatc acttctgctg
    22261 ctactacagc acaatggaag ggtcaccgaa tcaacatcat cgacacacca ggacacgtag
    22321 acttcacagt tgaagttgaa cgttcattgc gtgtacttga tggtgctgta actgttcttg
    22381 atgcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca acaacttatg
    22441 gtgtacctcg tatcgtattt gcaaacaaaa tggacaagat cggtgctgac ttcttatatt
    22501 cagtatcaac tttacatgac cgtttgcaag caaacgctca cccaatccaa ttaccaattg
    22561 gtgcggaaga cgacttcaca ggaattatcg accttgtaac aatgaaggct gaaatgtata
    22621 caaacgatct tggaacagag atcgaagaaa cagaaattcc tgaagaatac cgcgaattag
    22681 ctgaagaatg gcgcgaaaaa ttggttgaag ctgtagctga aactgatgaa gaacttactt
    22741 tgaaatactt agaaggtgaa gaaatcactg aagctgagtt aaaagaaggt atccgccgcg
    22801 caacagttaa cgtagaattc tacccagtac tatgtggttc agcatttaag aacaaagggg
    22861 ttcaattact attggacgct gttcttgatt acttgccatc accgttagat atcccagcta
    22921 tcaaagggat tgaccctaaa actgatgaag aagtagaacg tcctgcagac gacagcgctc
    22981 ctttctcagc tttagcattt aaagttatga ctgacccatt cgtaggtcgt ttaactttct
    23041 tccgtgtgta ctctggtgtt ttacaatcag gttcatacgt acaaaacgca acaaaaggca
    23101 aacgtgaacg tgttggtcgt atcctacaga tgcatgccaa ctctcgttca gaaatctctg
    23161 aagtttacgc tggagatatc gctgctgctg ttggtttgaa agacacaact actggggaca
    23221 cactatgtga tgaaaagagt ctagttattc ttgaatccat ggaattccca gaaccagtta
    23281 tccaagtcgc tattgaacct aaatcaaaag ctgaccaaga taaaatgggt gtggctttac
    23341 aaaaactatc tgaagaagat cctactttcc gtgctgaaac aaacgtggaa acaggcgaaa
    23401 caatcatcgc tggtatgggt gaacttcact tagatatcat cgttgaccgt atgcgtcgtg
    23461 aattcaaagt tgaagctaac gtaggtgcgc ctcaagtatc ttaccgtgaa actttccgtg
    23521 ctggcactca agccgaaggt aagtttgtac gtcagtctgg tggtaaaggt caatacggtc
    23581 acgtatggat tgaatttaca ccaaatgaag aaggtgctgg tttcgaattt gaaaatgcga
    23641 tcgtcggtgg ggttgttcct cgtgaataca ttccagctgt tgaaactggt ttgaaagatg
    23701 ctatggaaaa tggtgtctta gctggctatc cattagtaga tatcaaagct aaactttacg
    23761 atggttcata ccatgatgtc gattcaaatg aaactgcctt ccgtgttgct gcttctatgg
    23821 cattacgtgc agcagctaag aaagctaatc cagtaatcct tgaaccaatc atggctgtag
    23881 aagttgttat ccctgaagat tatttaggtg atgttatggg tcacgtaact gctcgtcgtg
    23941 gacgtgttga aggtatggaa gctcgcgctg gcggccaaca agttgttcgt gcgatggttc
    24001 cacttgctga aatgtttggt tatgctacaa ctcttcgttc tgctactcaa ggtcgtggta
    24061 catttacaat gacatttgac cactacgaag atgtaccaaa atcagtacaa gaagaaatca
    24121 ttaagaaaaa cggcggaaaa gctgaataat ttcttaaaac cggatagtcc tgcaaagaga
    24181 ttatccaaag agactatatt tttaatatag tttcgtgtaa aataattatc gatgatatgg
    24241 gcatgtggtg taccttgtgt acatcatata cctatcagac aaataaaaaa tatattgtat
    24301 ttttaggagg acattttaaa atggcaaaag aaaaatttga ccgttctaaa ccacacgtta
    24361 acattggtac tatcggacac gttgaccatg gtaaaactac attaacagct gcaatcacaa
    24421 ctgtactatc taagaaaaac ggcggccaag ctatggctta cgatcaaatc gatggtgctc
    24481 cagaagaacg cgaacgcgga atcacaatct caacagctca cgttgagtat gaaacagata
    24541 cacgccacta cgctcacgtt gactgcccag gacacgcgga ttacgttaaa aacatgatca
    24601 ctggtgctgc tcaaatggac ggagctatct tggtagtttc tgctgctgac ggcccaatgc
    24661 ctcaaactcg tgaacacatc ctattgtctc gtcaagttgg tgttccttac atcgttgtat
    24721 tcttgaacaa agtagacatg gttgatgacg aagaattact agaattagtt gaaatggaag
    24781 ttcgtgacct attaacagaa tacgaattcc ctggtgacga tgttcctgta gttgctggat
    24841 cagctttgaa agctctagaa ggcgacgctt catacgaaga aaaaattctt gaattaatgg
    24901 ctgcagttga cgaatacatc ccaactccag aacgtgacaa cgacaaacca ttcatgatgc
    24961 cagttgaaga cgtgttctca attactggac gtggtactgt tgctacaggt cgtgttgaac
    25021 gtggacaagt tcgcgttggt gacgaagttg aagttgttgg tattgctgaa gaaacttcaa
    25081 aaacaacagt tactggtgtt gaaatgttcc gtaaattgtt agactacgct gaagctggag
    25141 acaacattgg tgctttacta cgtggtgttg cacgtgaaga catccaacgt ggacaagttt
    25201 tagctaaacc aggtacaatc acacctcata caaaattctc tgcagaagta tacgtgttga
    25261 caaaagaaga aggtggacgt catactccat tcttcactaa ctaccgtcca caattctact
    25321 tccgtacaac tgacgtaaca ggtgttgttg aattaccaga aggaactgaa atggttatgc
    25381 ctggcgacaa cgtaacaatg gaagttgaat taatccaccc aatcgctatc gaaaacggta
    25441 ctaaattctc aatccgtgaa ggcggacgta cagttggtgc cggtgtcgtt acagaaatca
    25501 aagcttaatt cacttaagtt ttaaagtaaa gcaacattta ttcggacgta atcgctcgga
    25561 acgaaggtct taggatcttt gttccgagct tttttattta ttgcaggatc aggaaaaaag
    25621 acaggagtat tataaataga agaaacacga aataaaagag tcagtatttt ctaaatgaaa
    25681 ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt ttatatatat
    25741 tataatgaca aaggtgcgtt tactttagtg aattgcacag acgtaatcag aatgatttcg
    25801 tcggctaaga aacgagaggt tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg
    25861 gaaattttcg tggagctatg tctacttttc aaaataggcg aaggagggaa caagatggca
    25921 aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc gtattttaga tcaatcagcg
    25981 gataaaatcg tggaaactgc aaaaagaact ggagctagcg tgtctggtcc aattccatta
    26041 ccaactgaac gcagtcttta cacaatcatc cgtgcgactc ataaatacaa agattcacgc
    26101 gaacaattcg aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa
    26161 actgttgatg ctttgatgaa gctagactta ccatctggtg taaacatcga aatcaaatta
    26221 taaaactaaa acaaaaataa tggaggtgta ctcatgacca aaggaatctt agggaaaaaa
    26281 gtgggaatga cacaaatctt cactgaatct ggtgaattaa ttccagtaac tgttgtagaa
    26341 gctactccaa acgtagtact acaagtgaaa acaatggaaa cggatggcta cgaagctatt
    26401 caagttggtt accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt
    26461 gcaaaagcaa acacggctcc taagcgcttc attcgtgaat tcaaaaatgt tgagctagga
    26521 gaatatgaag taggaaaaga aattacagta gatgttttcc aagcaggaga cattattgat
    26581 gtaacaggta ctacgaaagg taaaggattc caaggggtta tcaaacgcca cggacaaagc
    26641 cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt
    26701 gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta
    26761 actatccaaa acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa
    26821 ggtaatgttc ctggagctaa aaaatcttta attactatca aatcagctgt gaaagctaaa
    26881 taattgtagg aagaaaggag gaactaagga atgccgaatg tagcattatt caaacaagat
    26941 ggaagccaaa acggtgaaat cacacttaac gaagaaatct tcggaattga acctaatgaa
    27001 agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg
    27061 gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca
    27121 ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc gtggaggtgg cgtagtcttt
    27181 ggaccaacac cacgttctta cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg
    27241 aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga aggcttgaac
    27301 ttcgacgcac caaaaacaaa agaattcaaa caagttcttg ctaacttatc tatcgactct
    27361 aaagtattgg ttgttctaga aaaaggtaac gactttgctg ctttatcagc acgtaactta
    27421 ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt atcaaataca
    27481 aaagtgttag caacacaaac tgctcttact caaattgagg aggtgcttgc ataatgaact
    27541 tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca
    27601 agaaatacac ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa caagcggtag
    27661 aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca
    27721 aacgtatggg caaatatgca ggatatacaa aaaaacgtcg caaagcgatc gttacattga
    27781 ctgaagattc aaaagaaatc caattattcg aagctgctga ataagcacta atagataact
    27841 cattaaatta ggagggaaaa acgtggcgat taaaaagtac aaacctacct caaatggtcg
    27901 tcgtaacatg acaggttctg attttgctga aatcacaaca tcaacacctg aaaaatcatt
    27961 gttacaacca ttgaaaaacc atgctggacg taacaacaac ggccgcatca ctgtacgtca
    28021 tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta acaaagataa
    28081 cgttgttgca actgttcaaa cgatcgaata tgatccaaac cgttcagcta acatcgcgtt
    28141 agtacactat gaagatggag tgaaagctta catcttagca cctaaaggac tagaagtggg
    28201 tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc ttccattaga
    28261 aaacattcca gttggtactg ttatccacaa cattgaaatg aaacctggta aaggtggaca
    28321 attgatccgt tctgctggta caagtgctca agtacttggt aaagaaggca aatacgtatt
    28381 gatccgcttg aactctggtg aagttcgtat gatcttagct gcatgccgtg ctacaatcgg
    28441 ttctgtaggt aacgaacaac acgaattaat caacatcggt aaagctggac gctctcgttg
    28501 gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac cctaatgatc acccacacgg
    28561 tggtggtgaa ggtaaagctc ctatcggacg taaagcacca gtttctcctt ggggtcaacc
    28621 agctcttggt tacaaaactc gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg
    28681 tcgcaaaaat aaataattac tttgaccatg tgtcgcacat tttgagagga ggttcaccat
    28741 gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc
    28801 tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt caacagtttt
    28861 cccaagtttt gttggatata ccatcgcagt ttatgatgga cgtaaacatg taccagttta
    28921 catccaagaa gacatggtag gacacaaatt aggtgaattt gcacctacaa gaacttatcg
    28981 tggacatgct gctgacgata agaaaacgaa acgctaattt gaggggagga taaatccaaa
    29041 tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa
    29101 aagcccgttt agtaattgac cttatcagag gtaaaagtgt cgcagatgca atttcaatct
    29161 tgaaattcac accaaacaaa tctgctggaa tcattgaaaa agtattgatg tcagcagttg
    29221 cgaatgcaga aaataacttt gacttagatg ttgaaaactt ggtagtaagc gaagcttttg
    29281 ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa
    29341 tcaacaaacg tacaagtcat atcacggtag tcgtatcaga aaaataagga gggataatca
    29401 gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca tcatccgcga ctgggatgct
    29461 aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg aagatttaag aatccgtaaa
    29521 tttatttcaa ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca
    29581 ggtcgcgtaa acatttcaat tcacacagct aaaccaggta tggttattgg taaaggcggt
    29641 tctgaagtcg aaagcctaag aaaagaatta aacaaattaa ctggtaaaag aatccatatc
    29701 aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat tagtaggtga aggaatcgca
    29761 cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat ccaacgctca
    29821 atgcgtgctg gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa cggagcagat
    29881 atcgctcgtt cagaaggata ctcagaagga actgttcctc ttcatacttt acgtgcggac
    29941 attgattacg catgggaaga agcagataca acatacggaa aactaggagt taaagtgtgg
    30001 atctatcgtg gagaaattct tccaactaaa aaaaacactg agaaaggagg gaaataatca
    30061 tgttagtacc taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag
    30121 ctaaaggcgg aaaagaagta gcattcggtg aatacggttt gcaagctgtt gattcacatt
    30181 ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat gactcgttac atgaaacgtg
    30241 gtgggaaagt atggattaaa attttccctc acaaatctta tactgccaaa gcaattgggg
    30301 tacgtatggg ttctggtaaa ggggcacctg aaggatgggt tgcaccagta aaacgtggta
    30361 aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag
    30421 cttctcacaa attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat
    30481 cgaatgaagg ttaaagaaat cagagaatta accactgccg aaatgcttga tcaagaaaaa
    30541 caattaaaag aagaattgtt taatcttaga ttccaattag caacaggtca attagaaaac
    30601 actgcacgta ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa
    30661 caagctaagt aattatggaa ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc
    30721 aaagtttatc aaggtcgcgt ggtttcagac aaaatggata aaacaattac cgttgctgtc
    30781 gaaacaaaga aaaaccaccc tatctatggc aaacgcatga actattctaa gaaatacaaa
    30841 gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt
    30901 ccattatcag ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc agttattatc
    30961 taataacacg agattttcct atatagattg aatgcaaaat ctgaaaggag gatacacaac
    31021 gtgatccaag cagaaagtcg tttaaaagtc gctgataact caggcgcacg tgaaattttg
    31081 acgatcaaag tccttggtgg atctggtcgt aaaactgcta acatcggtga tgtgatcgtt
    31141 gctacggtta aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc
    31201 gttatcgttc gaacaaaatc aggagctcgt cgtacagacg gttcatatat taaatttgat
    31261 gaaaatgctg cagtgattat tcgtgatgat aaaagccctc gtggaacacg tatctttggg
    31321 ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa
    31381 gttttataat cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa
    31441 ggcgataaag ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt tgtactagca
    31501 gcgttcccga aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa
    31561 aaaccttctc aagctgctcc tcaaggtgga attgttgaga tggaagcgcc aattcatgtt
    31621 tctaacgtga tggtggttga ctctactggt gtagctggcc gagttggcta caaagaagta
    31681 gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag ttttagataa ataaaagttt
    31741 aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact
    31801 ccatcattga tggaaaaatt taactatagc tctgttatgc aaacacctaa agttgaaaaa
    31861 atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt agataaagca
    31921 gttgaagaat taactttgat cactggtcaa aaaccaatga ttacaaaagc taaaaaatca
    31981 atcgctggat tccgtttacg tgaaggaatg ccaattggtg caaaagttac attgcgcgga
    32041 gaaagaatgt acgaattctt agacaaattg gtttctgttt cactaccacg tgtacgtgac
    32101 ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa actatacttt aggtattaaa
    32161 gaacaattga tcttcccaga agtagattat gatttagtag ataaagtacg tggtatggac
    32221 atcgttattg taacaacagc aaactctgat gaagaatctc gtgagttgtt gacacaatta
    32281 ggcatgccat tccaaaaata attaaaggag gcgaactacg tggctaaaaa atcaatgatt
    32341 gctaaaaaca aacgtcctgc aaaattttca acacaagctt atactcgttg cgaacgttgc
    32401 ggacgtccac attcagttta tcgtaagttc catctttgcc gtatttgctt ccgcgaactt
    32461 gcctataaag gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat
    32521 aagaaggagg taaaagatca atggtcatga cagatccaat tgcagatttt ctaactcgta
    32581 tccgtaacgc aaacatggtt aaacatgagt ctttagaagt acctgcatca aaaattaaac
    32641 gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag
    32701 atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta
    32761 tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag
    32821 taccaaaagt attgaacggt ttaggtatcg caatcatttc gacttctgaa ggtgttgtca
    32881 ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat
    32941 caagtagaat ctacaaggag gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt
    33001 atccctgaag gagtaacagt tactcaagac ggaaacaacg ttacagttaa aggaccaaaa
    33061 ggtgaattga ctcgtacttt ttcagcagat atcaaaatga atatcgaagg aaacgaagta
    33121 actttcactc gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg
    33181 aacttcaaca acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc
    33241 ggggttgggt accgtgcaca attacaagga tcaaaacttg tattgaacgt tggttactca
    33301 catccagtag aaatcacacc accagctgga gtaactgttg aagtaccagc taacacacaa
    33361 gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa cattcgtgga
    33421 actcgtcctc cagaacctta taaaggtaaa ggtattcgtt atgttggcga atatgtacgc
    33481 cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa gccagcagca
    33541 ggaaaaaggc atttggctga agtttacctt caaaaaaata catttttact tgtgccgcag
    33601 cttctatttt tcgaaacgta tttactgctt gaaatagtag tacaataaaa ataaagaggt
    33661 gacaattgtg attacaaaac cagataagaa caaaacacgt caaaagagac atcgtcgtgt
    33721 acgtaacaat atctctggta ctgctgagcg cccacgcttg aacgtttttc gttctaacaa
    33781 aaacatctac gctcaaatta ttgatgacgt agcgggtgta acgctagcaa gtgcctctac
    33841 cttggataaa gaaatttcag gtggaacaaa aacagaagca gcacaagctg tcggtaaact
    33901 agttgctgaa cgtgcagctg aaaaaggcat taaagtagta gtctttgacc gtggtggata
    33961 cctttaccat ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg gactagaatt
    34021 ttagaagaag gaggaatacc attcatggtt tatatcgatc caaaacattt ggaattagaa
    34081 gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt
    34141 tttgctgctt tagttgttgt cggtgacaaa aacggacacg tagggtttgg tacaggtaaa
    34201 gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt
    34261 gaagtaccta tggttggttc aacaatccca cacgaagtaa tcggtgcttt tagtggtggt
    34321 agaatcctaa tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg accagttcgt
    34381 gccgtattag aattagctgg ggtagcagat atcacttcta aatctttagg ctcaaataca
    34441 ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa
    34501 gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg gataaggagg acaacaataa
    34561 tggctgaatt aaaagttact ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg
    34621 atacagttaa agcgttaggt cttacaaaga ttaacagttc agtggttaag ccttctaatg
    34681 aagcaattaa aggcatgatc aatactgttt ctcacctagt ggacgttgaa gaaatttaat
    34741 ttagaaaaat cataactaga acattaagga ggtgccaaac ctatgaaact tcatgaatta
    34801 aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt
    34861 aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg
    34921 cgtttaggat ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa acgaggattc
    34981 acaaacgtga accgtaaaga ctatgcagtc attaatcttg atgtattgaa ccgctttgaa
    35041 gacggagctg aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa
    35101 gctggaatca aagtattagc taatggggaa ctaaacaaga aattaactgt gaaagcagca
    35161 aaattctcta aagcagctca agaagcaatt gaagcggctg gtggatcaat cgaggtgatc
    35221 taatgtttag actattaaaa gatgctttta aagtaaaaga cattagatcg aaaatcctat
    35281 ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca tattactgta ccaggcgtaa
    35341 atgctgcttc attgcaaaac ctttcttctt tgccattttt caacatgttg aacttggtaa
    35401 gtggtagtgc gatgcaaaac ttttccattt tctcaatggg ggtttcacca tacattactg
    35461 cttctatcgt cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga
    35521 aacaagggga agttggtcgt aaaaaactga atcaagcgac tcgatatctg acattagtat
    35581 tggcatttat ccaatctgtc ggtatcactg ctggattcaa tcaatacgct caattaggtt
    35641 tcgtgaataa tcctaacgta gcaacttacg taacgatcgg tttgattcta acagccggta
    35701 cgatgtttgt tacttggcta ggagaacaga tcacagaaaa aggtatcgga aatggcgtat
    35761 caatgatcat ttttgcaggt atcatttcta gattgcctga aagtatcaga gaaatttatg
    35821 aagattactt tataaatatt gatccttcag acatatggaa atctgctatt tttgtggcca
    35881 ttttactagt agcggtcttg ttgatcgtga cattcgtaac atttttccaa caagctgaac
    35941 gcaagatccc gatccaatat acgaaacgcg tagctggcgc gcctacaagc agctatctgc
    36001 cgttgaaagt aaatgcagct ggagttattc cagttatctt tgctagttcg tttattgcta
    36061 cgccaaatgc tgttttacaa gcattgagcg gtagattcgg ttctgaaaac tggtacaaag
    36121 tgatgatgga gatctttaac tataatacag cacctggtgc tacgatttat acgattctga
    36181 tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa
    36241 acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc tggtaagggg actgaagagt
    36301 atgtctcaag attactgatg cgtttaagta cagttggtgc agtcttcctt ggtttagtgg
    36361 cattgcttcc aatcattgct caaatggttt gggatcttcc acaatcaatc ggattaggcg
    36421 gtacaagctt actgatcgtt atcggtgtag ctttagaaac tgcgaaacag ttagaaggct
    36481 taatgctgaa acgtaaatat gttggattca ttgagtaaga aagcgtgttg aggtttcccc
    36541 tcaacacacg ttctcacaca tttattagga gggaaaacaa tgaacctcat tttaatgggg
    36601 ctgccaggcg caggtaaagg aacacaagct gaaaaaatca ttgatgctta tggtattccg
    36661 cacatctcaa caggagatat gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg
    36721 gaagcaaagt cttatatgga taaaggtgca ttagttcctg atgaagtaac aaatggaatc
    36781 gtgaaagagc gactagctga accagataca gagaaaggct tcttattaga tggctttcca
    36841 cgtacgctag atcaagcaaa agctctggac gcaatgctaa aagatttgaa taaaaaaatt
    36901 gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct agccggccga
    36961 tttatctgca gcaattgtgg agcaacttac cataaaattt tcaaccctac aaaagttgaa
    37021 gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga taaacctgag
    37081 acggttaaaa atcgtctggc tatcaacatc aaaaatagtg aaccaatctt ggcttattat
    37141 aaagaacaag gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc tgtatttgca
    37201 gatgtacaaa aaatcattga aaaatagcac gattgcccgt agccgacttg cattatgcag
    37261 ttactcttgt caagttcaat aaattatgat agaatatgtc agtccgactt ctagagaatc
    37321 ctctagatga tccattttga gaattttgag gtgggaactg cagtttccgc cattttatcg
    37381 tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag atatgattga
    37441 agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg aactagaaaa
    37501 cggacaccaa gttcttgcta ctgtttcagg taaaattcgt atgcattaca tccgtatctt
    37561 acctggtgac aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg gccgtattac
    37621 ttatcgcttt aaataattgt actccgtaaa actacaggag gtattatcat gaaagtaaga
    37681 ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg
    37741 gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag gataattgga ggtgctataa
    37801 atatggctcg tattgcagga gtagatattc ctcgtgataa acgtgtagta gtttcactta
    37861 cttatatcta tggtattggt aacactactg ctaaaaagat cttagctgat gctggtgtat
    37921 ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac agatgctatc cgtgcagaag
    37981 tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt
    38041 tgatggaaat cggttcttac cgtggaatcc gtcatcgccg tggattgcct gttcgtggac
    38101 aaaacacgaa aaacaatgca cgtactcgta aaggcccagc tcgcgctatc gctggcaaga
    38161 aaaaataatt cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta
    38221 aacgccgtgt gaagaagaat atagaagcag gtattgcaca tatccattct acattcaata
    38281 acactatcat catgatcact gatactcacg gaaatgcatt agcgtggtca tcagctggtt
    38341 cattaggttt caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa
    38401 ctgctacaaa agcagcaatg gaacacggat taaagactgt tgaagtaaca gttaaaggac
    38461 ctggttctgg acgtgaagca gcaattcgtt cattacaagc tacaggttta gaagtgactg
    38521 caattcgtga cgtgactcca gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg
    38581 tttaatgagt gccgctcatt ttgagtttta tcagtaacgt cattgaacaa gacacttttc
    38641 gttttgaaag gggtaaagat aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt
    38701 gatgaagaaa aagattatgg caagttcatc gttgaacctc ttgaaagagg gtatgggact
    38761 actttaggga attccctacg tcgtatttta ttatcttctt tgccaggtgc tgccatcact
    38821 aatattcaaa tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt tagagaagac
    38881 gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa
    38941 aaaacccttg aaatcgatat taccggacct gctacagtaa ctgctggcga tattatcgtt
    39001 gatagtgatg tagaaattct taacaaagat atgtacatct gtagtgtctc tgaaggagct
    39061 acgttccatg ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc agatgaaaat
    39121 aaaaaagaag atatgccaat tggcgttctt ccagtcgatt caatctacac acctgttcgt
    39181 cgtgtgaact atcaagttga gaacacacgt gttggacacc gtgaagattt tgataaattg
    39241 acgatggaga tttggacaga tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg
    39301 aaaatcatga ctgaacattt ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct
    39361 gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa
    39421 gagttagact tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat caatactgta
    39481 caagaactta caaataaatc tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa
    39541 tcattagaag aagttaaagc aaaattgcac gacttaggtt taggtttacg taaagaagat
    39601 taaaaccttt tacgaaggag ggaaaccaac gtgagttatc gtaaactagg acgcacatct
    39661 agccaacgta aagcgatgtt gcgtgattta acaactgatt tattaatcaa cgaacgtatt
    39721 gtgactactg aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat gatcacttta
    39781 ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt
    39841 gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat cagctttaca aaaactgttc
    39901 aacgatattg cacctcgtta cgcagaacgt caaggcggat acacacgcat tttgaagaca
    39961 gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta aaatcacact
    40021 aataacatca ctttatagat gattctttta gagtgttatg atgttggaag atatttttcc
    40081 gagtctagct cagcgctgct cctcaagcga atatcgcttg gggagtaagc actcatcata
    40141 aagtgttttt ttataccttc aattacctgt catgacagat atgtttgaag gcataaaaaa
    40201 acattttaag cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt agtaggaact
    40261 ttaggatgaa gaaggagtaa tcatggagcc aattattgaa ttagaaaaga taaattataa
    40321 ataccagcca gatgatcttc gccccgcatt gaaagatgtc tcctttacaa ttgataaagg
    40381 tgagtggatc gctattattg gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat
    40441 caatggattg cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa tattggatga
    40501 agaaaatatt tggacgatcc gacagatggt tggaatgatt tttcaaaatc cagacaatca
    40561 gtttgttggt tcgacagtag aagatgacgt tgcttttggt cttgaaaatc aagggattcc
    40621 cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt
    40681 tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa caacgtgttg ccattgccgg
    40741 cgtggttgcc ttacgacctg atatcattat tttagatgaa gcaaccagta tgctagatcc
    40801 agaaggtcgt gaagaagtga tctctacgat caagaagatc aaagaagaaa gtcaattgac
    40861 cgttatatct atcactcatg atattgatga agcagctaat gccaatcgga tactagtgat
    40921 gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa attttttctg ctggtcctga
    40981 actgatcgac ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga
    41041 acgtggggtt gatgtgccca gtgaatatat gacagaagaa aggatggtgg attggttatg
    41101 gacatccgtt ttgaacaagt agacttcact tatcagccga atacaccatt tgaacaaaga
    41161 gccttatttg acatcaatat gacgatcaaa gaaaacagtt atactgcatt agtagggcat
    41221 accggaagcg ggaaatcaac cttacttcag catttgaatg cgcttgttaa gccaacaagc
    41281 ggtacagtcc atattggaga acgagatatt cagccggata cggataataa gaatttaaag
    41341 cccatccgaa aaaaagttgg catcgtcttt cagtttccag aagcacagct attcgaagaa
    41401 acggtagcaa aagacattgc tttcggtcct aaaaactttg gtgtcagcga agaagaagca
    41461 ctagtcctag caaaagaaac attagaacaa gttgggctgg atgaaagcta tttggaacgt
    41521 tcgccatttg aactttcagg agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc
    41581 atgaggccgg aagtacttgt attggatgaa ccaacggcag gacttgatcc ccagggcaga
    41641 aaagaaatga tggagatgtt ttggcgtctt cataaggaac agcagatcac tattgtatta
    41701 gttacgcatt taatggatga tgtagccaat tttgcagatt atgtctatgt gttggaaaag
    41761 ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc aaaacattga atggctaaaa
    41821 gaaaaacaac taggcgtacc tactgccacc gagtttgcag aagaattaat ggctaaaggg
    41881 atgaactttg ctaccttgcc tttgacagct gaagaattag cagacgctat cgtccattat
    41941 gctggaggaa atgcccatga tgaataaatt gatatttggt cgttatatgc ctggagattc
    42001 cttgatccat cgattggatc cccgagctaa attattagcc agtttttatt ttatcggaat
    42061 cattttttta gcaaataatt gggaaacgta ccttttgttg gcatttttca cattactttc
    42121 tgtcttcttg tcaaaagtag atttgaaatt ttttatccga ggcattcgcc cattgatctg
    42181 gctaatcctc ttcactgtag ctttgcaggt cttgtttaca agtggcggag aaacttattg
    42241 gagttggggt gttttccata tcactgaatt tggtgtgatg aacggcctct ttattttctg
    42301 tcgattcgtg ttgatcattt ttatgtcgac tctactcacg cttactacac cgcctttgga
    42361 attatcggat gcgattgagt acttgcttcg tccattgaaa gccgtacggt tcccagtcca
    42421 cgaaatttca ttgatgctgt cgattgctct tcgttttgtg cctacattga tggacgaaac
    42481 agaaaagatc atgaacgctc agcgcgctcg cggtgttgat tttggtgaag gaaacctagt
    42541 tcagaaaatg aaagcagtca ttcctttgct gattccttta tttgtcagca gcttcaaccg
    42601 tgcggaagat cttgcgactg ctatggaagc aagaggctac aaaggtgggg aaggaagaac
    42661 gaaatatcgt gtattgcatt ggcataatag agacacgatg gttatcattg cttttgtatt
    42721 attaactgtg ctactgatct ttttaagagg ataaacgttc agtgggccgc tatctattct
    42781 tttcttgctg atctgtttgc aagaaaggat gcaagcagtg tatgacaaga aatattaggg
    42841 tctgtggcag actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa
    42901 ttttatagaa aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg
    42961 tacgttacaa agcaatcatt gcgtacgacg gaacaaattt taatggattc caaaaacagc
    43021 caaatgggcg tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa
    43081 aagaaatcac ggtctttggt tcgggaagga cagatgccgg agtacatgcg atcgggcaag
    43141 taatccattt tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata
    43201 cccagtcacc agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg
    43261 caagatattt ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca
    43321 gtccttttcg acgccattat gcaagttatt ttccttaccc acttgatttg gaaaaaattc
    43381 agcgggcgct tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa
    43441 gctcgataga ggataaaata cgtacgatct acgaggctaa gatggaggta aatgaaacaa
    43501 aagacgaatt gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct
    43561 tagtaggaac gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata
    43621 ttattgcgaa aaaagatcgt aatgcagcag gacctacagc acaccctgaa ggactttatt
    43681 tgtatgaagt gaaatatgaa taagaggtta tgatagaagt ggtcagcttc aagaaataag
    43741 aaggtattta cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagaa
    43801 gctacttctg gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
    43861 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
    43921 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
    43981 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
    44041 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
    44101 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
    44161 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
    44221 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
    44281 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
    44341 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
    44401 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
    44461 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
    44521 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
    44581 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
    44641 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
    44701 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
    44761 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
    44821 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
    44881 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
    44941 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
    45001 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
    45061 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
    45121 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
    45181 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
    45241 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
    45301 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
    45361 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
    45421 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
    45481 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
    45541 ccaaaatgat tcctaacaac agcccaccat cgtcttgttc tatcttttcc catccttttc
    45601 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
    45661 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
    45721 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
    45781 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
    45841 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
    45901 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
    45961 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
    46021 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
    46081 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
    46141 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
    46201 gaagataaac aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
    46261 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
    46321 caagaaacca aaaagccaat ccggcacagc cgaccttaag caaagtcaga acatacagtg
    46381 catctggcat catctggttt ggaaaaaaga acaccagtgg cgtgaagatc ccgcctagat
    46441 agtaagaaat taaagataaa taattcaatc ctagagaagc attccatgta taaaaaatac
    46501 tttgctttcc aagaagaaca tttcggaagc tggcatgaaa attagaaaac tgtgaaaagg
    46561 catcacttgc caaaatactt ctgctgcttc caggatagat tccgatactt agataaacaa
    46621 gtgctaaaat caaaaacgga atcaggaaac tagctgctgc gtagcgtccg ttttctctaa
    46681 agaatgactt taaatttttt ttcatattta ttttccacca aatttctatg aagttgtatt
    46741 cagtttatca tatccttgtt tttctctgct taaaaagagt ctagttttca caaaaaaata
    46801 aaatttagaa taaataactt cataatttgt tcgtataaca aaaataaacg caaaaaaagg
    46861 gtaggaatca aaagtcccac ccttaaaaat ttttattaaa taaaaaaccg ttcactatct
    46921 gaacggtcca agtatcattc aattaaacgc gttcaacttt tcctgatttc aaagcacgag
    46981 ttgacaccca aactttttta ggttttccgt cgaccataac gcgaactttt tgtaagttag
    47041 gtttaacagt acgttttgtt gagttcatag cgtgtgaacg gttgtttcca ctttttgtct
    47101 tgcgacctgt aaagtaacaa acttttgcca tggtaatttc ctcctttgcc ttgatttgga
    47161 gcaatagaat aattcagctc ataccagact aacatatcat atagaatggg aatttgcaag
    47221 tacattttca tagatacaat tgacaattga gaagaataat tgtaaattaa acaaggttat
    47281 acctgaaatt ttgcaaggtt tttgagtact ttgggtaact gccttttatt tcaagttgcg
    47341 ctatgataaa atatgataga agaaaaatgg gagtttttta cccgttaagg aggctcattc
    47401 atggctgtaa aaattcaaac gtcagctgga accattgaaa ttaccaatga tgtaattgca
    47461 acagttgttg gtggcgccgc gacagatgtg ttcggtgttg taggaatggc cagcaaaaat
    47521 caaattaaag ataatattac tgagattttg cgtaaagaaa attactcaag aggagtagtt
    47581 gtccgccaag aagataatgg tattgctgta gatgtttata cgattatcag ctatggtacg
    47641 aaaatctctg aagtttcccg caacgtccaa gaaaaagtga aatacaatct agaggcatta
    47701 ttaggcgtaa ctgccaattc agttaatgtc tttgtacaag gtgttcgtgt actgccagac
    47761 taggcaggag actggctggt aactcagcag tcgatctgaa ggaggattat ctaggtgagt
    47821 gtaacagaaa taagtgcaag ccagttccag gaaatggtcc aggcaggtgc gaatcgtctg
    47881 caagtaaatg cagaatacgt caattcatta aatgtattcc ctgtgccaga tggcgatacg
    47941 ggaacaaata tgaatctatc tatgactagt ggagctaagg cagtagtaga ttcaacctca
    48001 gaaaaagtag gagaattagc agcaatgctt tcaaaaggat tgctgatggg tgcgcgcgga
    48061 aattccggcg ttattttgtc tcaattgttt cgtggatttt ctaagcaaat tccagatgtt
    48121 gtctcattaa atgcttctga tcttgctgca gccttcactc atggagttga aactgcgtat
    48181 aaagcagtaa tgaaaccagt agaaggaacg attttgacag tagctagaga agcagcgaaa
    48241 gctggagaaa agaaagcaaa aagtactgat gatgtcgttg aagtaatgac agctgttgta
    48301 aaaggagcaa agcgtgcttt agctaaaact ccagacttgc ttccagtcct aaaagaagtc
    48361 ggtgtcgtag acagtggagg gcaaggactg ctgtttgttt atgagggctt tttaagtgca
    48421 ttgaatggag aattccaagc ggatgaaaca tatgaacctt ctccagcaga aatggatgat
    48481 atggtcaatg cagaacatca tcgcagtgtc caaggtcaaa tggcgacaga agatatccat
    48541 tttggttact gtacagaaat catggtaaaa atcggagaag gacctacagt cgatagtaca
    48601 ttcgattacg aagaattccg taactattta gatggtatag gagattcttt acttgtggtc
    48661 aatgatgacg agatcatcaa agtccacgtg catacagaaa acccaggaga agtcttgaat
    48721 tatggacaaa aattcggttc attagtcaaa atcaaagtcg acaacatgcg cttgcagcat
    48781 gagacgattt tggaacacga tgaagaagtg aacgagtttg cgaaaaaaga aataccacgt
    48841 atcccgtatg gtatcgtagc gatcgctgct ggtaaaggcg tccaagagct gtttgaaagt
    48901 ttaggcgcta attatgttat cagtggtgga caaacaatga atccaagtac ggaagacatt
    48961 gttaaagcga ttcaagaagt aaacgcagat aaagtgattg tcttgccaaa caataagaac
    49021 atcttcatgg cagctgatca agcagcagaa gtagcagata tcccagtagc agttgttcct
    49081 tcaagaacga tttcccaagg attgacagct atgctttcat tcaatgatca agcttcttta
    49141 gaagaaaaca aagaaacgat gacagaaatg ttgtctagcg ttgtgagcgg tcaagtcact
    49201 cacgcgatca gagatacctc aattgacggt gtagagatca aagaaggaga ctttttgggc
    49261 atgatcgatg gaaaaatcct gatctctaac ccagatatca cagcaacagc gttaagtaca
    49321 ctagaacaaa tgatcaatga ggacacagag atcgtaacga tcttggcagg tgatgaagga
    49381 acagttgaac aagcaaatga atttgctgag aaactaacag aaaaacatcc tgatttggaa
    49441 atcgagatcc accaaggaga tcagcctgtc tatccttatc tattttctgc tgaataattt
    49501 ctgtgtcaga atatctttct aacacagtat tcattcataa aaggccttcg gttagtgatc
    49561 aggtatgatc gctgaccaag tccttttttt gttaaaggag ggaggaaaaa tttatgcgtt
    49621 ctttagatga tccaatcact atgctggcag gagtcggaga aaaaagagca gctagtcttg
    49681 ccagcttggg catccaaacg attgaagact tactgactta ttatcctttt cgctacgaag
    49741 atattcagga aagaaatcta aacgagatcc aggatcaaga aaaggttact ttgaaaggaa
    49801 tcgtagtctc gcctcctgtg atgaatcgat ttggttataa aaaaagccga ttgcaatttc
    49861 gtatgatgca agaacatgat gtgttcaacg tttctttttt taatcaacct tatctaaaag
    49921 aaaaagtcat tttatcggag gaaatcgccg tatacggtaa atgggatgca aagcgtaaag
    49981 ccttgaacgg gatgaagatt ttaggttctc aaacaatgga tgacttttcg ccgatatatc
    50041 acgtcaacaa atcaattcgt caaacgacat tagttgatct aatcaaaaaa gggtttcaag
    50101 aatacggcac attgattgaa gaaaatctgc caaacaacct tgtggagaaa tatcgactgc
    50161 ttgaccgagc ttctgctgta agaagtatgc attttccaaa agaccacgaa gaaaaccatc
    50221 aagccaaacg acgtgtcgtt tttgaagagt tctttttatt tcaaatgcgt cttcaaggat
    50281 tgaaaaaagc agaaaaagca gaaacgaatg gtttggagat cctttatgat gtccaaaaat
    50341 tgaaacaatt tactcaaaag ctaccttttg aactgacaaa tgcacaaaaa cgtgtaacga
    50401 atgaaatctg tcgggatctt cgcagtccgc agcatatgca gcgacttttg cagggagatg
    50461 taggtagcgg aaaaacagtg gttgccgcga ttgcgttata cgctaccgtt acagcaggtt
    50521 tccaaggtgc gttgatggtg cctacagaaa tcttggcaca acaacatatg gaaagcttgc
    50581 aacagctgtt tgatcccaat gaagtaacca tcgcattgat tacggggtca acgaaagcaa
    50641 aagaacgacg tgagctcctt gaaaaactag agcaagggga aattaacgtc gtgatcggca
    50701 cacatgcatt gatccaagat ggagtcgaat ttcagaattt aggtctagtt attacggatg
    50761 agcagcaccg ttttggcgtg aaccagcgga aagtacttcg tgaaaaaggc tggcgaccag
    50821 atgtcttatt tatgacagcg actccgattc ctagaacatt agctattaca gcatatggtg
    50881 agatggatgt atctatcatt gatgaacttc cagctggacg tatcccgatc gaaacacgct
    50941 gggtaagaac acctcaattg gacagtgtac tggattggac gaaaaaagaa ctggcaagag
    51001 gacatcaaat gtatgtcatc tgtccgctga ttgaagaatc agaagcatta gatgtaaaaa
    51061 atgcagtgga aatctatgaa aaacttcgtg aactatttgc accaaaatat gaagttggct
    51121 tgttacatgg taaaatgaag aatcaggaaa aagaagtaat catggaagcc ttcaaagaca
    51181 accagatgca gatattagtt tcgactactg taattgaagt aggggtaaac gtgccgaacg
    51241 cgaccgtcat gctgatcatg gatgcggatc gatttggatt ggcacagctg caccaacttc
    51301 gtggacgtgt tggacgcgga agtgatgctt cctactgtat cttagttgct aatcctaaaa
    51361 atgaactagg tgtggaacgg atgaaaatca tgacagagac caccaatggt tttgtactaa
    51421 gcgaaaaaga tctggaatta cgtggtcctg gtgaagtatt tggcttccgt caatctggtc
    51481 tgcctcaatt tgcgattgct gacttggtga cggatggaaa tgttttggag gtagccagag
    51541 aagaggctgc agccctttgg caaataaagg attggcagat gcttcctgag tatcagcctt
    51601 tagcgcaaag cctccaaata gaaaatcagg ataatcgtta tttcgattag cagactgtgc
    51661 tatactttat actgatgacg gaaaaaggag tggaaacaat gaaaattgcc gtagatgcaa
    51721 tgggcggaga tcacgcacct caagcaattg tagaaggcgt ggctttagcg cagcaagatt
    51781 ttccagaagt agaattctta ctttacggaa aagaagcaga aatcaagaaa tacttaacaa
    51841 atgaaaaaaa catcacgatt atccatacag acgaaaaaat caatagtgat gatgagccag
    51901 taaaagcaat tcgacgaaaa aagactgcat caatggtcct tgctgcacaa gcagtgaaaa
    51961 acggtgaagc agatgctgta ttttcagcag ggaatacagg cgcgctacta gcagcagggt
    52021 tgttcatcgt aggacgtatc aaaaatatcg aacgccctgg cttgatgtct accttgccag
    52081 tagtagggaa agaaggtgcc ggtttcgata tgctggatct tggcgctaac gcagaaaaca
    52141 aaccagaaca tttgttgcaa tacggtattt taggttcttt ttatgcacgt aaagtccgag
    52201 gtatcggacg tcctcgtgtc gcactcttaa acaacggaac agaagcaaca aaaggtagtg
    52261 aagtgacgaa aaaagcctat gaactccttc agaatgaaac aagcttgaat tttattggaa
    52321 acgtagaagc tcgtgagcta ctcaatggtg tggctgatgt agtggtcaca gatggattta
    52381 caggcaacgc tgttttgaaa tctattgaag gaacagcaat gaatatgatg aatctattaa
    52441 aatctgctat tctaaatgaa gggatcaaag gaaaaatggg cgctatgctt ttaaaagacg
    52501 gcttacgttc tttgaaagca gaaatggatt attccaaaca tggcggtgcc gttttgtttg
    52561 gcttgaaagc accagtcatt aaaactcatg gagcaacagg ccccgatgct gttcgctata
    52621 cgatccgcca aatccacaca atgcttgaga cagatgtagt tggacaatta gtcgaacaat
    52681 ttgaaaaaag cgaagaatag tatttggata tctgaagcga tccgttccat tgaaaaaaag
    52741 gtggacattc ctgaaaaatg ccagtaaaat tagccatggt gtaggtaatt gacaagtgta
    52801 tgattcttgg aggtgaaaaa gttgactcgc gaagaagtat ttaacaaagt agcgaaaatt
    52861 atttcaaatc attttgagat agataccgat aaagtgacgg atgaattgaa catcaaagat
    52921 gatttaaaag ctgattcaat tagtattatg gaattcgttt tagagcttga agatgaattc
    52981 ggtacggaaa tttcagatga agacgctgaa caaattgaaa cagtcggtgg cgcagtagat
    53041 tatatcagca gccatcttaa ataatttcaa ataaaagcaa agaaggataa aaagctgcat
    53101 gcagcttttt atccttcttt ttttaatctt tttgacacgt aaaagtcatt t
//
LOCUS       DANJJH010000005        50915 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000005 DANJJH010000000
VERSION     DANJJH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            36..881
                     /locus_tag="PWS53_000333"
     CDS             36..881
                     /locus_tag="PWS53_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0870608.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1162..2382
                     /locus_tag="PWS53_000334"
     CDS             1162..2382
                     /locus_tag="PWS53_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL0870609.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2379..5099
                     /locus_tag="PWS53_000335"
     CDS             2379..5099
                     /locus_tag="PWS53_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0870610.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5083..6027
                     /locus_tag="PWS53_000336"
     CDS             5083..6027
                     /locus_tag="PWS53_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0870611.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6096..7106)
                     /locus_tag="PWS53_000337"
     CDS             complement(6096..7106)
                     /locus_tag="PWS53_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0870612.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7286..7630)
                     /locus_tag="PWS53_000338"
     CDS             complement(7286..7630)
                     /locus_tag="PWS53_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0870613.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7623..8057)
                     /locus_tag="PWS53_000339"
     CDS             complement(7623..8057)
                     /locus_tag="PWS53_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL0870614.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8195..8932
                     /locus_tag="PWS53_000340"
     CDS             8195..8932
                     /locus_tag="PWS53_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870615.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8925..10136
                     /locus_tag="PWS53_000341"
     CDS             8925..10136
                     /locus_tag="PWS53_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870616.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10340..11122
                     /locus_tag="PWS53_000342"
     CDS             10340..11122
                     /locus_tag="PWS53_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL0870617.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11192..11854
                     /gene="trmB"
                     /locus_tag="PWS53_000343"
     CDS             11192..11854
                     /gene="trmB"
                     /locus_tag="PWS53_000343"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL0870618.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11952..13706)
                     /gene="ade"
                     /locus_tag="PWS53_000344"
     CDS             complement(11952..13706)
                     /gene="ade"
                     /locus_tag="PWS53_000344"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL0870619.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13838..14767
                     /gene="tadA"
                     /locus_tag="PWS53_000345"
     CDS             13838..14767
                     /gene="tadA"
                     /locus_tag="PWS53_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL0870620.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14724..15773
                     /locus_tag="PWS53_000346"
     CDS             14724..15773
                     /locus_tag="PWS53_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL0870621.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15770..16048
                     /locus_tag="PWS53_000347"
     CDS             15770..16048
                     /locus_tag="PWS53_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL0870622.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16023..16493
                     /locus_tag="PWS53_000348"
     CDS             16023..16493
                     /locus_tag="PWS53_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL0870623.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16510..16812
                     /locus_tag="PWS53_000349"
     CDS             16510..16812
                     /locus_tag="PWS53_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870624.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16829..17200
                     /locus_tag="PWS53_000350"
     CDS             16829..17200
                     /locus_tag="PWS53_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL0870625.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17172..17531
                     /locus_tag="PWS53_000351"
     CDS             17172..17531
                     /locus_tag="PWS53_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870626.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17703..18710
                     /locus_tag="PWS53_000352"
     CDS             17703..18710
                     /locus_tag="PWS53_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0870627.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18735..19919
                     /locus_tag="PWS53_000353"
     CDS             18735..19919
                     /locus_tag="PWS53_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL0870628.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20356..21939)
                     /locus_tag="PWS53_000354"
     CDS             complement(20356..21939)
                     /locus_tag="PWS53_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0870629.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22517..22660)
                     /locus_tag="PWS53_000355"
     CDS             complement(22517..22660)
                     /locus_tag="PWS53_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL0870630.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22882..23361)
                     /locus_tag="PWS53_000356"
     CDS             complement(22882..23361)
                     /locus_tag="PWS53_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0870631.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23434..23718)
                     /locus_tag="PWS53_000357"
     CDS             complement(23434..23718)
                     /locus_tag="PWS53_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870632.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23796..23990)
                     /gene="ssrS"
                     /locus_tag="PWS53_000358"
     ncRNA           complement(23796..23990)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWS53_000358"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24062..25348)
                     /locus_tag="PWS53_000359"
     CDS             complement(24062..25348)
                     /locus_tag="PWS53_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL0870633.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25486..26157
                     /locus_tag="PWS53_000360"
     CDS             25486..26157
                     /locus_tag="PWS53_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL0870634.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26123..26611
                     /locus_tag="PWS53_000361"
     CDS             26123..26611
                     /locus_tag="PWS53_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL0870635.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26624..27571
                     /gene="prmA"
                     /locus_tag="PWS53_000362"
     CDS             26624..27571
                     /gene="prmA"
                     /locus_tag="PWS53_000362"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL0870636.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27572..28336
                     /locus_tag="PWS53_000363"
     CDS             27572..28336
                     /locus_tag="PWS53_000363"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL0870637.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28569..30782
                     /locus_tag="PWS53_000364"
     CDS             28569..30782
                     /locus_tag="PWS53_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL0870638.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30793..31239
                     /locus_tag="PWS53_000365"
     CDS             30793..31239
                     /locus_tag="PWS53_000365"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL0870639.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31410..32669
                     /locus_tag="PWS53_000366"
     CDS             31410..32669
                     /locus_tag="PWS53_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL0870640.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32758..33705)
                     /locus_tag="PWS53_000367"
     CDS             complement(32758..33705)
                     /locus_tag="PWS53_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0870641.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34099..35391
                     /locus_tag="PWS53_000368"
     CDS             34099..35391
                     /locus_tag="PWS53_000368"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL0870642.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35406..37175
                     /gene="aspS"
                     /locus_tag="PWS53_000369"
     CDS             35406..37175
                     /gene="aspS"
                     /locus_tag="PWS53_000369"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL0870643.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37284..38159
                     /locus_tag="PWS53_000370"
     CDS             37284..38159
                     /locus_tag="PWS53_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0870644.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38425..40308
                     /locus_tag="PWS53_000371"
     CDS             38425..40308
                     /locus_tag="PWS53_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0870645.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40383..40667)
                     /locus_tag="PWS53_000372"
     CDS             complement(40383..40667)
                     /locus_tag="PWS53_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0870646.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40657..41391)
                     /locus_tag="PWS53_000373"
     CDS             complement(40657..41391)
                     /locus_tag="PWS53_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL0870647.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41520..42143
                     /locus_tag="PWS53_000374"
     CDS             41520..42143
                     /locus_tag="PWS53_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL0870648.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42335..43456
                     /locus_tag="PWS53_000375"
     CDS             42335..43456
                     /locus_tag="PWS53_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL0870649.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43463..46591
                     /locus_tag="PWS53_000376"
     CDS             43463..46591
                     /locus_tag="PWS53_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL0870650.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46610..49804
                     /locus_tag="PWS53_000377"
     CDS             46610..49804
                     /locus_tag="PWS53_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL0870651.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49950..50657)
                     /locus_tag="PWS53_000378"
     CDS             complement(49950..50657)
                     /locus_tag="PWS53_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0870652.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16658 a         8914 c        10597 g        14746 t
ORIGIN      
        1 ataagcagtg aatttataat caacgtatag gagacatgga tgaaatgaaa ctaattggta
       61 tagatttaga cggtactctt ttgaattctg accagagcat tagtcaaaaa aatgcaagaa
      121 caatgaaaca tttaccggaa gactgttttc cattcatttg ttctggaaga gaagtagaag
      181 acattaataa tattctcaaa aaatctggtt taaggatacc agcagtaggg ttaaatggtt
      241 ctgttggatt tgatggagaa aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa
      301 aaaaaattga ttctgtccta tcgaaatttc caaccaaaat atatacaaat ctcggaagtt
      361 atgaatctca gaattataaa aataaaatga aaaaaatttt tcacgaggta gggagtgaat
      421 tttctatcaa agagctaaac tatgagcttg agtatgaaaa atctatttgt tcaatccctt
      481 ataataatat tgaggaaatt atagaaaagg atgctatcag catttacaaa ttttttattt
      541 ttattcctaa tcgtcaattg aaagctaata tatttaacca tttgaaaaag atgctaaatg
      601 tgtctgttac tgaatcggca cctgtaaata ttgaaatcgt acctcaaaat gtatctaaag
      661 gtgtggtgtt tgatcgtttg gaatcaatct acaatttaaa gaaacctttg agaatcgcga
      721 tcggagatag tttaaatgac ttatctatgt ttgaaagtgc agatattagt tttgcaatgg
      781 gaaatagtca tcaagaaatc atgaaaaaag ctgattatgt cactgtttca aatgataaag
      841 acggagtatc tcaagcattt gaaagaattc ttaccatata aactaattaa ggtagtatag
      901 aacatagaat tctaaatacg gaaaaatgga gcataaattc cagtacaaat agtacttctt
      961 cagtgtcaaa cgaagcgaat ataacataga tatttaggat tgtgacaaaa atcatgtcac
     1021 aatctttttt tcgaataaac ttattttttg aagtcggcca tttatcgtaa tttctttgca
     1081 taatacgaac aaattttcgg ttattgatga aaaaaaacat tgaaaatggt atgataaaag
     1141 aaaaaaagga ggaaattttt aatggtgaaa tttatccatg cggcagattt acatatggat
     1201 cggtcatttg aaggactgac aactcttgat aaaactgtac aggaaaaact attgaagacg
     1261 aatctgaccg ttttatcaaa tatcattgaa caggcaataa taaataacgt tgattttgtt
     1321 ttacttgcag gagataattt tcatcaaaat cgtccttcat taaaaataca gaaacatttt
     1381 tcagaacaga tgaaacggtt agaaaagaac catatacctg tatttataat attcggaaat
     1441 catgatttct atcaaaaaga gcggtattgg ttctcctttc ctaaaaatgt ccatttattt
     1501 acatccgaaa cggtagagac aaaaaaaata acgataaaaa gtggagaaac tgtctctctc
     1561 agtggattta gttatcgtca accgtggatt caaaaagaca aagtgatgga atttccttct
     1621 agagaactca cagattacca tattgggttg taccacgggg aacctggcgt ctctcaaaaa
     1681 ggaaactatg ctcctttcca accctcaaag atgcaagaaa agggatacga ttattgggct
     1741 ttagggcata tccatgtgcc aacggtctta aatgaaaagc aaacaattgt ttatccaggt
     1801 gcgcctcaag gtcatactaa aaaggaacaa gcttctacat ctattctact agttgaatta
     1861 agtaaaggaa gttgccaaat acatccgatt aaagtagcag aggtctattg gaagacaaag
     1921 gaaatatcat tgaggaaagc aagaacgaca aaagaggtca ttgttcacat tcgtcagcag
     1981 ctttcaaaag tagaggatgg atttagttta attgagataa aattaaaaga ttatgaccat
     2041 ctcaatacag atgttttgga acggatacaa tcaggagagc tattagacta tttgttagag
     2101 gaattctcag ataggataaa tgatttcttt atttggcgta tttctctcat agagaatact
     2161 ttttatacga agaccccact tgctgcttct gctaaattga tggaacagtt atttcaatac
     2221 tatcagacac ctcaagattt ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg
     2281 gctagaatat tgagcgaact gccggaatat caagaagaaa cacttgaaaa agccaagcag
     2341 ctgcttaatc aagatttttt atttgaggag gatcaggaat gaagatttta aggatcgaga
     2401 ttgctgcttt tggaaaatgg cgtcaaaaaa gttttgactt ttattcaggc aatcagctga
     2461 tctatggggg aaacgaagca ggaaaatcaa cgatttatca atttattcaa gcaatattat
     2521 ttggtttccc ttcaaaaggc cgtaaaaaaa aggattatac accaaaagac ggctcagcct
     2581 atggcggcaa aatatggctt aaacatcctg tatatggtga atttgctgtc gagcgctaca
     2641 agcagcaaaa tcgcggaaaa tcgaaggtgt ggctggggga tcaagtggga agtgatgagt
     2701 tactagaaaa actgatcgca cctttgacga aagagttatt ccagcaggta tttactttcc
     2761 agcaagagca gttgatggaa ctagatcatt tgagagaaaa agagctacat gatgcgctga
     2821 tttctttagg catcacgggc agttctcaag tattccaaaa gcggcaagaa tattatcaga
     2881 aagcacaaca attattcaag aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg
     2941 aatggaaaga gctgcaagaa aaaattcatc aaaaacaaga acaggaagct tattttggca
     3001 agttgatcca acaagaacag gaatacgagt cgcaacagca gcagttattg gcacaattaa
     3061 aagaggcgaa caaacaattt tcattagcca gacagcaaga acttaatttt tctttatatg
     3121 aagaatggca atctctcaaa aaaaacagca ctacaggtct tcctgatgag caacttcttt
     3181 tagatctgga gtctttctcc aaacggtatc agcaaacaaa tgagcgtttg gaagaattag
     3241 aagcaacact ggaaaagcat agcgggatgg atcagcagtc agccagatat tatttttatt
     3301 tagagcagga gcaggaacta aaagatctcc agcaacttca atttacagcg gaacaattaa
     3361 acgaagaatg gcaaagatta caaattgaga ctagagaaac aaattcagaa ttgaagattt
     3421 tggaacaaag gtggcattgg cagaaaaatc cgttaccgga agaattttta tatgaagaag
     3481 aatggaagca tcttctggct gaagaaagaa ggattgctga tctagaaagc cagatttctg
     3541 tgcgtctcca aatagctaaa gaacagcaag agatgattga agcagaagtg aatgacttgg
     3601 aagagaccta tccagcatta ttacaaaatc agtcagagat agcaccagtc tcttcatcta
     3661 aaccgtatat gctgatggct ggcggagcaa taatcattgc aggtatattt gtaccaatgc
     3721 ccttgaaagt gatcctttta ctaattggtg caggcatgat tggctatttc ttctacaaaa
     3781 ggcaaccaaa aattaaaact aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa
     3841 aactaggaca acttgatcta gccttagctc aattagctga agcagaagaa aaacaaaaga
     3901 gaactggaga agaaaaacag attcttcagc aagaagtata tcgttttgcg gatcatcggc
     3961 acttaggaaa gatggatacg ctcaaactca tgaaagaata tggacaggaa gtcatagatt
     4021 atcagaaatt atatggcgta tttgagcgga aacataaaag agaacaggaa atacaagaaa
     4081 aactacgaca aatcgaccaa aaatttgagt ttgttaaaga atggttaccg atacaagata
     4141 agacaatttc agaaaaaatg gtattgcttt cccgttttat acaagagatg gaacagatga
     4201 aatttgccag aagctatcag caaaatactt tattgaagca agaaatcaat caattgaaaa
     4261 agaaacaaaa agtatggata caagaataca gcgaacaatt gactcaagca ggcttatctt
     4321 atccttcgga agtacctgtc tttttacaaa aagccaaaca agccgaacag cagatgaaaa
     4381 gatacaatga attatcggaa atgcttactc cactatttcc tgaagagctg acaaaagaga
     4441 aattatctgt aagactgcgc caactgaaag aaaagcaatc ccaactccag caagaagaaa
     4501 aagaagcaag tgaaaatcgg caacgactcc aacttcaagt agaagagttg caaagagacg
     4561 gcacattgga cgaactttat caacaagcta gccggcaaaa agcaaaatta caggaattac
     4621 tggaggaatg gggagcactt gaaatcgccg ggacattctt aggtgatctt tcaacagaga
     4681 tgagtgaaag acaattaccc cagttgttaa gagcagcagg tgattattta ggtattttaa
     4741 caaaaggaaa ttatcagaag atattactga aaaatgatac actgacttta acagacggat
     4801 atacagaatt tcccatctat gagttatcta caggtacgaa agatcagctg attatggcga
     4861 tgagatttgc ttacctctca ttagaagcta accgttcgat atgcccgatc atcattgatg
     4921 atggctggct tcattatgat catcagcgaa agtatcaatt agctgaactt ttagctaaat
     4981 ttggagaaaa gtatcaaata atttgttttt cttctgataa agaaatggta agctattacc
     5041 atgagatgaa ccaaatcgta aaacaattag aaggagcaaa taatgaaaaa aattcgtgat
     5101 ttagtaatcg atgagcagtt tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc
     5161 gctaaaaacg gaaaaaaatt tattgctttt actttccaag atacttcagg tactatcgat
     5221 ggtaaatact gggatgcttc ggaggaggaa atcaataaat ttacagccgg cacagtcgtt
     5281 tttctaaacg gtaaaagaga ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg
     5341 cgcttaacaa gggtagatga gccaggagag cctagtttat acatggaaag agcccctttg
     5401 aagaaagaag aaatggaaga agaaatcaat caattgttgt ttgagatcac caatgcgcat
     5461 tggaatcgta ttgttcgcta tctacttaat aaatatcaaa aacaattttt tgagtttccg
     5521 gcagcaaaaa gaaaccatca tgcatttgcc agtggattag cttatcatac tttgacaatg
     5581 cttcacttag ggaaatcaat cgccgatgaa tatcctgagc tgaatcgagc attgttgtat
     5641 gctggtatca ttttgcatga tttaggaaag gtgattgaac tttccggtcc tatgtcgaca
     5701 gaatatacgc ttgcaggaaa tttgatcggt catctggtat tgattgacga agaaataact
     5761 aaagcctgtc ttgcgttgaa aatcagcgaa gctgacgaag atgttcttgt attacgtcat
     5821 atggttttag cacaccatgg cttattagaa tatggctcac ctgtacgtcc aagaataatg
     5881 gaagcagaaa tattacatca aatcgacaat atcgatgctt cgatgcagat gatgttgact
     5941 tcgattcgtc aaacagaacc aggaaaatac accgatcgga ttttcggtat ggacaaccga
     6001 agtttctatg taccaaaaga tttatagcaa aaatttattt ttttcattca aaacaaaaaa
     6061 agcgagacat aagtcttcgc tttttttgtt ttcttttatt ttgatgaatc agttgtttct
     6121 tcagttgaag attcttttgt tgaatcagtt gattttgtgt cagaactctt tgttgaagct
     6181 gtagtttcac ttgagtcttt cgtacttgat gaagttgttg tgaatgcact caacacattt
     6241 tcaaaggcat catctttgat ttttacattt gcatctttta attcttcacc gattacttta
     6301 gttgtgaatg cattgtcact tagtttagtt tcagtagcga tatcttttag ttgatctttg
     6361 tatttatcca tgtcattgcc tttattttgg tttttcacca ttttgacaac gtagtattct
     6421 gtcgcataag aagtaggatt tgttgtagta ataacatcgg agatctcacc gtctttcaat
     6481 ttgaaagctg cttcttttac ttctgcagga atagttgttg tcgttgagtc gaatttaact
     6541 ttaccgccat cttcttttgt ctctgtatca gttgatttgt ctttggctaa tttagagaaa
     6601 tcatctccat catccgctga ttttttcaca tcttttgctt catcttcact tgataatttg
     6661 atgatttgtg cttctacttc tggatggaaa gatttccaag cagttttcaa atcgtcatct
     6721 gtaatatcta cgtgagcttt caagccagct tcaaaagcta aattgttacg aatatattct
     6781 ttataagtat ctttcgtata tccagcagct tctaattgtg attcaaaagt atcacctaat
     6841 gatttcgctt gtttgtcgta ttctgcatcc acttgcttat cggttacttt atcgccatat
     6901 ttgttattga atactttata aatgatcatc cgttgtacta aggattggtt cgatgattca
     6961 agttttgctt catcatagaa atcagataca gtgatcgttc cgcctttcat cgttgcgata
     7021 tctttattag tatcgcctga acaagctgct agagttaaaa cagccaacgc actagtagct
     7081 gccaaaataa ttgatttttt tttcattaaa atttgcactc ctgtaattag ttattatttt
     7141 tatatattaa ctataccata tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac
     7201 aaaattcaaa atttcatcac attcctagga aatacgcaaa cttttctcac ttatatagtt
     7261 aaatctgttg agaaaataat cattattata tttgttcagg atactctggc agatcagctt
     7321 ttagtttttc tacttgttca ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat
     7381 cttcgatatc ttttttgatt gcttcttgga aagcaggaat cacagattca gctgtttctt
     7441 ttgtttcgat caatgcgctt ttaaaatgat tcaggctgtt atttaaatca tttgtcagag
     7501 ttgttgcttc ttccaattct tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg
     7561 ccaataacag acctccgaca gtgccagcag cagctccaaa taacagtcct ttgaaaaagt
     7621 ttttaatcat cgcaggacac ctgctttacg attgtttctt gaatagcttt tagttcttcc
     7681 gatccataac tatcttgatg attgacaaag tgaatagaaa aatcatcctc tttgctgtag
     7741 cgagggacta aatggatatg tgaatgaaaa acagattgat aggctagttc acggttgtta
     7801 ttcaagatat taagaccttt catttctgga aaagcttttt ccaatgcttg agcaatctta
     7861 ggcacacgcg aaaaaacctc tcctgcaagt tcgggatcat attcaaaaat atctgctaca
     7921 tgacgtttag gcacaactaa tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca
     7981 tagaccttgt cgtcttcata tattttatag cttgggattt cttgattgat gattttgcaa
     8041 aaaatacagt tttccatata aaacatcctt tctataaaga ataaatactt cagcttaact
     8101 ttaccataag ttaaagctat tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt
     8161 ataataaata aaaataaatg gcggaggaaa aagaatgagt ttaattatcg atcatgtcac
     8221 aggggggtac ggacatctcc ctgtgttaaa agatatcagt ttcgatgtac agcctggaga
     8281 gatagtaggg ttgattggac tgaacggagc agggaaaagt acgaccatca agcacattat
     8341 cggtctgctg gaagcttcaa aaggaacgat agaaatcaat catctatcct taaagaaaaa
     8401 tacggaagaa tatcgcaaac aaattgggta tattccagag actccttctt tatatgaaga
     8461 attgacattg agagaacata tcgaagtcac tgcgatggcc tacgatatac ctatggatat
     8521 agcaatgaaa agagcagaaa agttgttgga gacttttcgg ttatctaatc gtcttgactg
     8581 gttcccagct aatttttcaa agggaatgaa acagaaagtg atgatcttat gcgccttttt
     8641 gatcgagcct tctttatata tcatcgacga accattttta ggcttggatc cactagctat
     8701 caatgcattg cttgaattga tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc
     8761 tacgcatatt ttagctactg cagaaaaata ttgtgatcgt tttgtggtct tacataacgg
     8821 agagatacga gcagacggtt ctcttgagat gttgagaaaa gaatttcagc tgccggattc
     8881 ttctttggac gaaatctata ttgctttaac aaaggagcag gcacatgagt agtttttttc
     8941 aaaaacgttt agcaagacat caaaagaaaa tgatgaaata tatgcgttat gtattgaacg
     9001 atcattttgt tttagtttgt cttttcttga ttggcggtgt gggattatat tattctaatt
     9061 ggttgaaaac cctgcagcca ccgtttgcaa ttggagggct cataatctct gtattttgga
     9121 tgctctgttt attcgtgggt aagatggcta cttttgcaga ggcagcagat attgtttttt
     9181 tacttcctaa agaaaaagaa atgcaaacct atttggaacg agcttttcgc tattcttgca
     9241 tttttccgtt gatctttttg atattggcat gcggattggc catgccatta gtggtggtta
     9301 gtacggagca atccttttcg atgtttttcg tctatctgat catattatgg agtttgaaat
     9361 tcagtcatct gcaaatcaag agagcagaat tgtttcgact agatcaacaa gttattcgtt
     9421 attggaaatt aggctggttt ttttcttctc tgatcttttt gtttctatca ttttattggc
     9481 taatatgggc aggaatagca ggtgcattag tacaggcaag tctatattac tggtttttgt
     9541 ggaagcaaca agaaaaagga ttggattggg agaaaatggt gcatgtagaa gaacataggt
     9601 tgcatcagat ctatcaattt atcaatttat tcacagatgt tcctgaaatt acggcaaaga
     9661 tcaaaagaag acgctatttg gatcctatat tgactcatat taaaagagaa tcaaaaaaca
     9721 cttatcttta cttgtttgca agaagattgg caagaggttc tgattttagt ggtctttatt
     9781 tccgattagt aattataggt gggttattga ttgccggagt agtcgacttt tattttagtc
     9841 tgattatcgg tttcttattt ttatatttga tcggtttcca actgttacct ttagcgaacc
     9901 agttccgcta tatggttctc gttcagcttt atccaatcca taagaatcag aagaagcagg
     9961 cgattcagta tctactcaca tgggtattaa ttatcgcgtc ggttattttt ggtttgattg
    10021 ctgcagtcgt attagatggg cagcagcgat gggttccgct agcggcttat ttgattgtta
    10081 ctatgctctt tgttaaacta tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac
    10141 aaagctgtgt agggtaaaaa tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt
    10201 cgttctattt tttttgagaa aagctggttt tttgaatggc aattaaatga aaaagaacgt
    10261 atatgtgctt gacgtgtagt gttcaggcga ctagaataat aagtactgca aaacaaagag
    10321 taagaggacg ggagaatgca tgatggagtt tcagttagac caagagtggc ggttacgtcc
    10381 catcaaaggc gacacaggaa aaacatacat aggcatgcgt gatgaggaca aagtctttat
    10441 taaacgaaac acgactccga tgttggctgc tctttcaagg gaaggaatta caccaaaact
    10501 tgtttggaca aaacgaacgg ggaatggcga tactttgaca gcacaagagt ggctggaagg
    10561 tcgcttgctg actcctaatg agattggtca aagaaacgac gtgattgatg ttttgtacca
    10621 tttacatcat tcaaattctc ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac
    10681 gccggagaag atgctgcaaa aatatgaaaa agaattaccg gaagaactcc agaaaaatca
    10741 atttctgcaa attgtccatc gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa
    10801 cttatcagta gtacacggag atgtaaacca tcgtaattgg ttagtatgta aaaattattt
    10861 atatctagtc gattgggatt caatcatgtt tgcagaccct gcggtggata ttgcaacgat
    10921 tttaggaaat tatgtgccac tgtctagctg gaatcagtgg ctgatcagtt atggcatacg
    10981 cccgactgac gaaatgcttg aaaaatttca ttggtatgcg gccatgaatt tgctccaaga
    11041 aatcaccaga tattgcaaac gtgaagatca tcgacggatg aacgaagaaa ttttacaatt
    11101 aaaacgaatt ttcagtggct gaggtttggc ccaaactctg cggagatttg gccagacctt
    11161 tttaattaga ggaaaggaat ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc
    11221 ggaaatgtta gcggctcacc cgaattttgt aattagtgat ccaacattgt ggaagggaaa
    11281 atggaatgaa ttgtttgaaa acgatcatcc aatccacatt gagattggta tggggaaagg
    11341 ccagttcatc actggaatgg caaaagccca tcctgaaatc aattatattg gagtagaaat
    11401 gcaggtcagt gttgtttcga ttgcactaga caagttgatc gaacaaccgc ttcctaacct
    11461 gaaacttcta catgtggatg gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga
    11521 tcaaatctat ctgaattttt ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt
    11581 gacatataag acttttcttg ctgtagatga acagattctt cgtccaaatg gagaaatcca
    11641 tttcaaaaca gataatcaag gattgtttga gtattctctg gcaagttttt ctcaatatgg
    11701 catgatcctc aaacaggtat ggttagattt acatcaaagt caatttgaag gcaatattat
    11761 gacggagtat gaagaaaaat tttcttcaaa aggtcaacgt atttatcgag tagaagctca
    11821 atttcaagat aagaccaaaa agaaaagtct gtgaaactaa ctttctgctt tcataaaaac
    11881 attaatatcc ataaataaat ctcagaaaat gaacattaca gatgtaatgt attttctggg
    11941 atttatttga tttaaaatgt atttatttca tcggtttttc cacaaggtgg aatcaattgg
    12001 aagcgagaaa atgaaaataa tcctttgtcg gtgatcttca attcgggtat gacagataat
    12061 gttaagaaag aaagtgtcaa aaaaggatca aaggttttag ctgctcctag ttgatgtgct
    12121 ttagttatta attcattcaa ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt
    12181 ccgcctatag gaagagaaac acaagctatt tcctgctgtt tgttgacaac ggtcatcccg
    12241 ccaccttttt gaatcaaaag attagcagca tacagcatat cctcgtcgtt tgtcccaacg
    12301 acaactagat tatgcgagtc atgggcaatc gttgtcgcta tcgctccttg tttcagctga
    12361 aatcctttca ctatgcctaa tccaatgttt cccgtttgat ggtgacgctc gatcaccgca
    12421 atctttaatt ggtctttttc gattgaagga atgaatcgtt cgccttgtcg gtccactttt
    12481 tctaccaaat ctttagtcac tagactattc gggataattt cgatcacatg ggcttgtgaa
    12541 gaagaaagaa gcagctctaa cgaatgtgga tttaatggtt gagcgtttag ttttggcaga
    12601 caacacgtat atgaatttga atgatcagca aataaatggt cttggagttg cccagattga
    12661 acaactaatg ttcccttata gaaaacttca tgaattggca gcttggtaag atcgtctgtt
    12721 aagaagaaat cagcttgata cccaggggca atcgcaccta gtgttttcaa atcaaaacat
    12781 tctgctgcat tcaatgttgc catctgaatg gcttgtaatg gatcgattcc aagtgcaata
    12841 gcttgacgga cgatatgatc gattgatcct tcttttacta aatcattgat cagtttatca
    12901 tcggtgacaa agaagcatct tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc
    12961 agatcttttg caacggtccc ttctcgaacc attaaataca taccaagatc caaacgttct
    13021 ttagcttctt ttgcagttgt agcttcatga tccgtccgaa tacctgctgc aggataaaca
    13081 tttaactctt ccatgccgat accagctgca tgcccgtcaa tttttttctt atgctggtgc
    13141 atcaattgga tcttttctag gatgtctgtt tcattatttg ctactgcttg atagttcatg
    13201 acttcagcta agcctaaaac ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt
    13261 aaatttgcac cattggtctc aaaaggggtt acaggtacac aagaaggtag cataaaaaag
    13321 atattcatga gtgtttgtct tgcatcttct atcataaact caagacctgt actcccagca
    13381 atgtttccta tttcatgggg atctgtcact acagtagtaa ctccatgaag caaggatact
    13441 tttgaaaatt cgcttggtga taaaagtgag ctttcgatat gcacatggcc atcgatgaat
    13501 cctggcacta gatattgccc attagcatta tgtacgcttt ttccttcgta ttcaccgatt
    13561 cctacgatca ttccttcgca gattgctaca tctttttcca tgatttcttt cgtaaagacg
    13621 ttgacgatct tagcatgctt gatcactagg tcagcaggct tttttttact tgctacttcg
    13681 atatatttcc ttaatgttgt ttccaatgga gtgcctccca agataaattt ttataagtat
    13741 aacataaatt cggcttttgc caatattttt ttgtaaattc attttatttg aaacaaaaag
    13801 atcaaaaaga cgtatgtttt ttgtggaggg gagaataatg gaaataaaac gattatcgga
    13861 tcaaatgata agtgaaagtt ttgccaaaca tgcccaagac gtatatatat tgccagcttc
    13921 agaagtattt tacatctatt ttcgctatgg aaaaaaacga acattctgga agaaagtctc
    13981 ttcggcagat ggacagcaac tgatctcaag atttaagtat ttgggacaga tggatatcgg
    14041 agaaaaaaga aaagctcagt taggtgcatt tacttattct ttagaaaaac aggaagttcg
    14101 tctgcggttg tcttctgtag gagattacct tcaacgagag agtttagtga ttcggctatt
    14161 atacggtact tttgaaacac cccatcgttg ctttagttct cgagattttg caactatttt
    14221 acagaaaacc gtcagacgtg gattatacct tttcagcggg acagtcggat caggcaagac
    14281 ttctctaatg tatcggttgg ctttggaaga aaaattgcaa gtgattacta tcgaagatcc
    14341 tgtagagata gaagaaccgt tttttctgca gctacagatc aatccgaaaa tagcacagac
    14401 ttatgataca cttttgaaac tcgcattacg gcatcgtcca gacttgctaa tcattggcga
    14461 gataagagat gaatcaactg cacatgctgc ggtaagagca gcactaacag ggcaccgggt
    14521 ttttgcaacc gtacatgcac gcaatcttga aggtacattg gtcagaatcc atgaactgac
    14581 aaagaaaaaa gaagagctat ctgaatgttt agctggtgtc atttatcaag aacttttgga
    14641 agatgccaat caccatcctt ctgttttatg gagttatcaa tttatggagg aagaatcagt
    14701 aaaaaaatca tgggaggaaa gttatgaaga agctcaggaa aatatttggc agtcagaact
    14761 attctaaagg attaacaaga agccagcaaa accagtttgc acaaaccatg ggagaactgc
    14821 ttttatctgg ttttagtctg caagaatccc tttatttatt actcacgatc cgttcgtttc
    14881 cacctgttat tttggcaaat atccaacatc agctaaagga agggcaagcg tttagtgagg
    14941 tactttcgca aataggatat ggagcagagc aactggtaca gatcgagtta gctgaacgtc
    15001 atgggaatct gccgacaact ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat
    15061 ttcgcaatga tttgaaaaag ctgcttgcat atccgatgct attgctcacc tttttgctag
    15121 gtatcttgtt tagtatgcgg ttgttcgttt tgcctcagct tattggttca ggaatggtca
    15181 tgcaagagca ttggggtatt cagctgatac aagtctttca ttggtatgtt ctactattca
    15241 tcctagcggt tactctattg gctttactag taaggtatcg tctgatgaag ttgtctcctg
    15301 ttgatcagag tgaatggata agcagacaat tattgattgg accgatatat tccttgtatc
    15361 aatcggctta tttgtcacta gagattggaa agctttttca tgaaggtttc gaattaaggc
    15421 aaatcatagt ctgcttaaag gaaacgaaac aagaaagtct gattcagcat cttgcctcta
    15481 agttgattca aggattggag gcaggtatgt cattggctga gcagttccag aattatcgtt
    15541 ttctatcaga agaattcagt aaaattgttt tacagggaga ggcaaaggga agtttaggta
    15601 aagaactgat tttttacagc gaatggacaa gaaaacagct tttcaaacgt cttcatcact
    15661 ggatgcattt tattcagcca atcatttttt tgggcgtagc ggctttgatt ttattagttt
    15721 atgccgctgt tttattacct gtttacggaa gcatagagga ggttttgcca tgaaaaagag
    15781 aaaaatatct tattatagcg gttttacatt gatcgaaatg ctaatcgtct tactgatcat
    15841 cagtgtatta gtgttgttgt tcgtccctaa tttgtcgcgt tataggaatc atgtagacca
    15901 agaaagtcgt gaagcgatca tccagttggt agacacacaa aaagagctct atgcgcttca
    15961 aaataatgga agagttccta ctgtggagga attgttgaat gaaggctata tcaaacgaga
    16021 acatgcagaa atctatcagc ggccttgaag gtaaggtttt agcatcagga tatacgttgt
    16081 ttgaatcact cttactgatt ttattactta caacgatcct ttcttttcca gttctcgcat
    16141 tttctgcatg gaagaatgaa ttggcagtct atcaattttt cggccaattt gaaaaacgaa
    16201 tctattctac gcaaaaaatc gctattgtga atcagatcca aacaggcttt tattggaata
    16261 gtgaagagaa tcaaatcatt tttcatgttc cgaatccaga taaaataaat tgggaagtat
    16321 tagatattcc tgatgagatt accttgaaac gccatgcttc tattactttt gctgctagaa
    16381 ccgggaatga aagttcacta aaagcttatc aattttattg ggaagaaaaa gagcggacga
    16441 ttacgtatca attccagata gggagtggtc gctatacaaa aagaattgaa taaaacagga
    16501 tttgttttgt tggagtccct tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg
    16561 agctttggcc agctgttttt gcttcaggca gaaaagaaaa gtggggagga actgctattt
    16621 attcgaatga tttatgaaga tatcaagacg cagcggatgt atcacaaaag accagagcga
    16681 agggaagaaa tgatcccata cgatatccaa attgtcgata ataatggagt aatcgaaaaa
    16741 gtgtttgtca atatggagaa agaagcgatc agtattgaaa aaacaagtaa ttccagcatt
    16801 tacgctgatt gaatgtctaa ttggactgat gatattgagc agtctattgc tcacattcaa
    16861 tcttttgatt cagcaaacca tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga
    16921 gtggctaata tttttagcac aattagaaga agaactcaag agcagtcaca atgtctctgt
    16981 gaaaaatcag caactgcact acacgatcga ggataaacag tatatcatcg aaagatatca
    17041 ggcaatgata cgtaaaagaa gaacttccgg cggacatcaa cctatgttaa caagtatcac
    17101 tgagcttcaa ttattggaaa aagaacacac aatttctttt tatgtacact ttgaaaatgg
    17161 agaagatgga tatgccatat ggacaaaaaa cgatcagtag aaaatgttat aagggctcta
    17221 ttttattttc tgtcttgctt ttatttcttt tatgtacgtt tttattgatg gcagcattag
    17281 aaagctaccg tttgtcaatg gatcttaata tacgaatcaa aaattattat atagcaaaaa
    17341 taatgaagca gatggcttta tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt
    17401 tttactacac aactggatct gtaacatatc agtgggtaca acagtcccta ttcttagagg
    17461 tggaagtctc accgtttatc tttcggttca cagaagaagt aaaaaatgat acagatacta
    17521 gtacagaatg atggaatgtt cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa
    17581 taaaaaaatg tttgccgttt cacaaataaa aaacagtgaa aacgattgaa attttgtcaa
    17641 ttgttcgata gaattaaaca ggtgaaaaaa ttgttataat atatctagaa atgaggtgca
    17701 ccatgttacc agaaaaaatg gaacaagggt ttaatcaaaa cctggaagct attcagcttt
    17761 tacagaatgc attgggaact tcctttttag aagcctatgt cgaaaatgca gaaaatttaa
    17821 tagatgatta tcaagtacgt gtggtggatg gagtgccgac aaaagagaca acgcaacgga
    17881 tcacagctct ttatgaagag ttgaagaaac tttctttcga gccagaagaa tggcgcagac
    17941 tgtctcaatt acttttattg aagggcagcc agacagaaca tttgcaggcg aaccatcaac
    18001 tcactcctga tagtatcggt tttttatttg tttttcttat tgaacaacta tacacaaata
    18061 aaaaagcacc ggttaagatt ctggatattg cggcaggaat gggcaacttg ttattaacag
    18121 ttcttttaaa cctatcgaat gcggggtacc aaacagaagg aattggtgtg gatattgatg
    18181 atacattgtt ggctgtcgca gcttctacaa gtgatttgac acaagcaaat gttcaatact
    18241 ttcatcaaga cgggttgcag gaattattaa tagatcctgt cgattttgca atcagcgatt
    18301 tgccaatcgg ttattatccg aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag
    18361 aaggccatag ttatgcacat catttattgc tagagcaatc aatgaaatat gtgaagccgg
    18421 atggttttgg tttgtttttg atgccgagtg gatttttaga aacggatcaa agtgaagaaa
    18481 tcaaaaaatg gtttaaagaa gaaggttatc ttcaaggaat gattcaattg ccagatgaat
    18541 tgttccgtaa taaacaatcg caaaaaagta ttttgatctt acagaaaaaa ggaccacaag
    18601 cacaacaagt aaaagaagtc ttgcttgtga aacttgcttc attaaaagaa ccagagaagg
    18661 tgacggaatt tttcaacgag tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg
    18721 agaaggagag tcatatgtct aaaacaattg caatcaatgc tgggagttca agtttgaaat
    18781 ggcaattata tcaaatgcca gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg
    18841 gtttacaaga ttctattttt accatcaagt acggcgataa tcaaaaattc gaacaaatca
    18901 tggatatcga aaaccacgaa gtagcagtaa aaatgctgtt agatcagttg attgaactgc
    18961 aaattttaag ctcttatgat gaaatcaccg gagtagggca ccgtgttgtt cacggaggag
    19021 aaacttatgg ggattcagta gtgatcgatg agaaagtaat ggaccgcatt gctgaattag
    19081 cagaatttgc tcctttgcat aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga
    19141 ttttacctga cattttgagt gtggctgtat tcgatacatc tttccactca acaatgccaa
    19201 agcaaaatta tttatatagc ttaccggtgt cttattatga agatttcaaa gtaagaaaat
    19261 atggcttcca tggcacaagt catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca
    19321 aaccgatcga agaactaaaa atcatcactt gccacttagg aaatggtgta tcgatcacag
    19381 cagtcgatgg aggtaaatcc atggatactt ctatgggatt cacaccatta gctggggtga
    19441 caatgggtac acgttcaggt gatatcgatc cagctgttct cccatattta atggaaaaat
    19501 tagagattga tattgatgaa atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc
    19561 taacaggatt gtctagtgat atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc
    19621 gtttagctta tgacattttc gtggaccgca tccgaaaata catcggcggt tatgtgacaa
    19681 cgatgaatgg tgttgatgca attgtcttta ctgctggtat cggcgagaat gacggacatg
    19741 tacgtagtga aatcatcaaa ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga
    19801 atgaattgcg tggtgaagaa gtagatatct ctacaaaaga atcaaaagta agagtattag
    19861 tgatcccaac agatgaggaa ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag
    19921 ccaaagcatt cgttaggata aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt
    19981 ttttttgcgg agaagaccgc aaaatggtaa tttaacttca atttcccttc ttttttacag
    20041 gatcttctta gggaattttg cttggaaaag tggtcaaaag ggaaaagact ggcattttat
    20101 ttattgattt tcaggaaaat cccaatattt tattttcatt catctttata agggacatta
    20161 catatgtaaa aagaaaaaac atttttagat cagcctcgtg ggctgtcgac tttattcttc
    20221 gctttatgtt tagctagaaa accgatccga aacattcatt gatcgaataa taaaaacttg
    20281 attttccacc tgtactttgc ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg
    20341 ttgcgaaaaa acgtcctagt caatcagttg gccgagcggc tggcggctga tctgctcctg
    20401 atcaataacg agtgcttctt ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc
    20461 taggagcgtt ccttgatagt cttgcagcat ttcttcaatt tcttctcgag catagctgtc
    20521 gaggtgattg gttggttcat ccaaaatcag gacgttgatt ttttctaaaa agagtttcag
    20581 caacgccaaa cggatttttt caccaccgga taaatctttg atccgtttac ccacatcgct
    20641 ttgataaaag ccacttttgg ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt
    20701 gatggcgtaa gccagcaaac ggctatcggg atcggcaaac tgcagctgct gcggcaaata
    20761 accgatttgc aagctactac ctaatttgat ggtgccaccg taaggctttt cctcaccgag
    20821 taataatttg agtaaggttg attttcctgc accattgtcg ccgatgatgg ctaaatgatc
    20881 tccacgatag actgcaaaac tgctgtcttt gaatagtact tgatcagcgt actgctggtg
    20941 caagttttga atgatgaaaa cttctttgcc agattttcca ccattagaca agctttgcag
    21001 ccgatttttc gggggaggtg gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag
    21061 ttctttggct tttttgaaga aggactcatt gtctccttca tgtgcccact gacgaaaacg
    21121 acgaatcatg actttcaacc gctggacttc tttttgctgc agttcgtaat tttttgtcag
    21181 ctcagccaag cgagcttctt tcaagactcg aaaacggctg tagtttccgg gatactcaat
    21241 caatgcacct tcttccagtt cccatgtttt tgtggtgact tgatcgagaa attgccgatc
    21301 atgagaaacg accagaaccg atttttttgt attctgtaaa tagttttcca accagcgaat
    21361 actgttaaga tctaaatgat tggtcggttc atcgagtaaa agtaaatcgg catcggctgc
    21421 taaaattttg gctaaagcga ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc
    21481 ttcctgacag tgtggaatcc ctaagccttg caagatcgct gcaatccggt cttctaatac
    21541 gtaaccgcct agttcttcaa agcgatgctg cagctcaccg taacgggcca gacacttctc
    21601 taagtttgta tctggttctg tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc
    21661 ctgtatctca gcggcagaat ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag
    21721 ttcttgttcg accgtcccga tctttaagcc ttttttgcga ctgataacac cgctgtcgac
    21781 accttctctg ccggtcaaga tttgtaaaaa ggttgatttg ccagttccgt tgatatcgac
    21841 taaggcaatt ttttcaccag cttcaatttg acaggataaa tcctcaaata atggattccc
    21901 gccgattgtt tttttaatag aaattgcttg tatgatcatg atctctctcc ttgagaccac
    21961 tcaaaaaggc catggttatc cctggcctgt gttatttaaa ctcagattat catgagaaaa
    22021 ataccttgag atggccattg attttacgtg aattcaactg ttttggacaa acttgtcccg
    22081 tcaacagcaa ctgtaaaacc aattgcatgg ctaaatgcga tatggtatcg ttggtaattt
    22141 tcattacttg ttattttagt catgcgcatc atagtatttt ccctccttcg tgtagttgtc
    22201 ttttccagtg tacctgaaag tgtcagaaag ttcaactagc gctgacattt acttaatatt
    22261 tatcgatgtg taattctgtc agagagcttt ttttatctga ctgagggtaa tgtctcgtgg
    22321 accacatcat tgtattctga gctaaaccag cgttcaggcg aaatcatatg gtgtttgata
    22381 aaaaactttg tctacatgac ttccttttgc catttgctta ataccgttgc attaaacagc
    22441 tcttctcttt ggaaatctca ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc
    22501 aagcggtagc ctttcatcaa ttcttgaatg atttaccgaa tgtagcactt gccttctcgc
    22561 ggtacccgaa cccgaaataa aattttagtt tggcgataaa atcggtatcc gccatcgata
    22621 cttcatattt tttcgtatca gattcatcgg aaacttccac attgacgaac caataagctt
    22681 tggcatgctt aggtcgtttt aaaccacacc agggcgacta atcgccctgg tgttcacttt
    22741 ttttatttta cttgaactca ttgactgttt tccctttatt ttaaacatta taaagaccga
    22801 tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt gtgcaaaaaa acctgccgat
    22861 agttggcagg tcgtctacgt gttaagcttt ggtgtggtct ttagctggca attggttttc
    22921 taaatcagtt cggacaacaa gcacatcgca agctgcgttt ctaataacat attcagaaac
    22981 agaaccaata aataaacgtt ctacagcatt caatcctgtg gctcctaaca taatcaaatc
    23041 tacttgatta tcttctggga tttctctggc aataatttgt tttggtgagc catattctac
    23101 gacagaggtt acattattca agccagcttt cttcgcattg ctttcataat ctgccaatgt
    23161 ttgctttgcc atttcagtag cttgttctgc aagcattcca tcaaaggatg aaacggtttg
    23221 gaaagaacgt gtgtcaatga catgtgctaa tagaagttct ccgttatttc ggatagctac
    23281 attgaccgcc tttttaaaag ctaattcagc ttcatcagaa ccatctacgg caaccatgat
    23341 ttttttgtat tgttgtaaca taagcattca ctcctcaagt caatcgtatt gtactctttt
    23401 attgtagaac actttaggga aaaagagaat tatttactgc tttggttcag caattttttt
    23461 tgcaaatcgg atactgaagg tggcagaaac aataattacg atcagtaaaa agaccaaggc
    23521 aataaagcgt tgattgaaaa aagctgttaa aatacccatt acgcctaaaa cagcatatgc
    23581 agcaccgtaa aattgtagga atccttgatt tttatcattt ttgttgatca atacaaaaaa
    23641 gatatcctgt ttattaaata aataatagct aacgaataat aatactaaag tggcgataat
    23701 cattaaaatc ctaatcatga acaaatcctc caaaataagc aacaaataga aaaagcaatt
    23761 tgccaagagc aaattgcttt attctttaaa agatggtaat ctccgttaat gccgttgttt
    23821 gtcctgtagt attgatcccg cgaattcaag caggtgggtc ccatggtcgt agcttcgaac
    23881 taccaaatga taaggcatgt taccagcagt gacacaagac gggtcccaag atatcaataa
    23941 aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc acagatttcc cacctctata
    24001 gtagcatatg aatttaaaaa gagcaatgag agagctcgca gaaacagaac cgttttcttg
    24061 tctagttttt tggaggatgc tttttccact cttttatacg gtaatactgt tgacccagag
    24121 cctgttcata tttccctgta tcttttggct gataatactg agcattcttc agtttatctg
    24181 gtaaatattg ctgattgacc caagcctgat caaaatgatg aggatactga taacccacgc
    24241 cacggttgag tgatttcgca cctttgtaat ggctatctct caaatgatca ggaacatctc
    24301 cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg
    24361 attttggcga caagcaaaga tcgacgacga cgtctgctag tgggatacgt gcctcaggca
    24421 atcccaattt ttcagcagct aatacagcgt tcactgtacg agccgcagca gcaggattac
    24481 ctaaacctat atcttcataa ccgataacca tcagacgccg gcaaatactt gccagatcac
    24541 ctgcctcaac taaacgggcg agataatgaa gtgcagcatc cacatcactt ccacgaatcg
    24601 atttttgaaa agccgaaata acgtcataat gagcatctcc atttttgtca tgggtaagag
    24661 cttttctttg gacacattct tcaatgatcg acagtgtaag atgaatcctt ccttctttat
    24721 ctgaaggagt cgatagtgta gccagttcca gcccattcaa tgcactccgc aaatcgccgt
    24781 tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa ctggattgct tgttgtccaa
    24841 ggccgcgttc tttatccctt aaagcatgtt cgactgccaa ctggatgtct tgttctgtta
    24901 atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc tggattgatg gtaatataag
    24961 gattctcagt agtcgctccg atcaagatga ttcggccact ttctaaatga ggaagcagaa
    25021 aatcctgttt tgttttatct aatcggtgta cttcatccaa taagaggatc actgtaccac
    25081 tcatttttgc ttcttccgct acaacttgta gatccttttt tgaatctgta gcggcgttta
    25141 gcatgcgaaa tgcataatta gtagagccgg cgatcgcact tgcgatactc gtttttccag
    25201 taccaggagg tccatataga atcatggaag agagcatgcg agcatcgacc atgcggcgta
    25261 tgatttttcc ttccccgact agatgctgct gaccaacgac ttcatcgatc gttcgaggtc
    25321 tcatgcgata agccaatggc tgttgcatag gattttcctc cttcttgttt ttacgtccct
    25381 taattatatc atatttagca agatgcctag aaagaacagc cttctgttgt tttgttttca
    25441 tagcttatcc aactaatatt gtgtatgata aagtaggtga gaaaaatgac ggataaacaa
    25501 aagatagatg aatttttttg gaataaaacg acgccagaag tggcacggga tttattgggg
    25561 atgtatttgg agcatgacac accagaagga agattagctg gatatattgt agacgcggaa
    25621 gcatacctag gtccagaaga cgaggccgcc catagctatg gtttaagaag aacaccgcgt
    25681 gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt acacgatgca tactcatcgg
    25741 attttgaaca tcattactca gccagaaggg attcctcaag gagtaatgat acgtgcaatc
    25801 gaacccgccg caatgattga tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat
    25861 atcagcaacg gtccgggaaa attagtggaa gcattagcaa ttcctcaaga actttatggt
    25921 cagtctatag cagatagttc gttacgctta gtttttgaga aaaaaaagac accaaaaaag
    25981 atcaatgctt tgcctagaat cggtatccca aacaaaggtg tatggacaga aaagccgttg
    26041 cgttttgttg tgtcgggaaa tccttatatc tctttacaaa gaaaaaacca aatcgagaaa
    26101 gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat
    26161 aattgaaaaa caagtgacag actatgatgt agctttagat tgggatacaa aaaatcatac
    26221 gatcgagatc gttgttcgct tgtttgcaga aaataaagca caatttgaga ttgatgatgc
    26281 agaagggatc gtttcgcaag aaccaattat cgaatttgaa gacggcgtac ttctttttaa
    26341 tcctcagaaa tctgtttttg atgaacgcaa ttatctagca gtaatccctt atgaaggaaa
    26401 aaaagggttg gcaaaatcag tagctgattc tttagtggaa tatctaaatg aagtattagc
    26461 tcaagggcaa agcgatttgc ttgatttttt ggatgaagac gatgacgaag ctgtattcga
    26521 gcttcactgg gataatcaac aactagcaga attagtagaa gtaaaacagc aaaatgaagc
    26581 agatacttat ttaccgtacc caagttatta aggagtgaca gatatgaaat ggaatgaagt
    26641 aaaagtagaa actgaaagtg aagcagtcga agcagttgct aatattttga tggaggcagg
    26701 ggcgagtggt gtcgcgattg aagattcttt agatgttgaa cattttcagt cggatcaata
    26761 tggtgagatt ttagataaaa atgattttac atcgctgaaa gaaggcgcac tagtcatggc
    26821 ttattttccg gaaacgactt tcttaccgga aatcttgcct tttatcaaag atcgtatcac
    26881 acaattacct gagtttggtc tagctattgg aaaaaataaa gtaacagtta gtgaagtaga
    26941 ggaaagcaac tgggcaacag cctggaagaa atactatcat ccggtgcgta ttacgcgaat
    27001 gctgacggtt gcgccaagct gggaaaacta ccagacagaa gatcctctag aaaaaattat
    27061 cacactagat cctggtatgg catttggcac aggcactcat cccacgactt ccttgacatt
    27121 gacagcacta gaaacagtct tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc
    27181 gggcgtgcta agtattgcag caaaatatct tggtgcaaaa gatgtctatg cctttgattt
    27241 agacgaagta gccgttcgtt cggcgaaaga aaatatggat atgaatgaaa tagccaaaga
    27301 tgtccatgta tcagctaacg atcttttgac aggaatcgag attgaaagtg atgtaatcgt
    27361 tgcgaacatt ttagcagata tcatcttatt gatgatccca gatgcttggc gtttactgaa
    27421 gcaaacaggc acgctgatcg tttcaggaat cattgaagag aagaagcaaa tggttctgga
    27481 tgccatgtta gaacaagggt ttatcgtgga tcaaattttc cagcaaaaag actggtatgc
    27541 aattattttg aaaaaaccag aggtggaata gatgcagcgg tattttttaa aagaatcata
    27601 tgaatcaaaa gactattttg ttttagaagg ggaaaactat caccacattg tacgggttat
    27661 gaggatggaa cccgaggatg aagttttttt ggcattcaat gatcaaatag ccattgttgc
    27721 agaaatcacg gagattactg aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa
    27781 tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc tatcctaaag gggaaaagct
    27841 ggattggatc gttcagaaat ccactgaatt aggagcgcaa gcttttcttg gtttcccggc
    27901 atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg aaacgacaac aacggcttga
    27961 aaagattgct caagaggcag cggaacaatc ccatcggcag atcgtgccat cagtagagtt
    28021 attggaaacg aaacaagatt tatttgagcg gctagatgat tatgacgtgg tcttggttgc
    28081 ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac
    28141 aaaaccagat agtcgaatct tagctgtctt tggaccagaa ggtggattat cccgcgatga
    28201 agtcgattat tttgtagaac acaaagggat actttgtgga ttaggaccaa gaatccttcg
    28261 tacagaaaca gcacctcttt atttattaag tgctgctagc tatcaatggg aattaatgaa
    28321 tccttctttc agctaacgtt cttcattaat aaaagagtat cgacagaaag aattgaaaaa
    28381 atagtcaaca atttgtataa tagaaacaat tatttataca atggatacga aaaatcgttt
    28441 gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg tataactgca cggattttgg
    28501 gacaattatc aaacagatat ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat
    28561 cgattcctgt gacaaaagaa gaaattttaa cgggacccgg tgtcataaag ctcgttagtc
    28621 aatacatggg tcccgaacat gtagcatttg ttgagaaagc atgcgaatac gcgactgctg
    28681 cacatgacgg acagtttaga aaatcaggcg aaccctacat tattcatcct atccaagtcg
    28741 caggtatatt agcagattta aaaatggatc cccatacagt ggctacaggc ttcttacatg
    28801 atgttgttga agatacagaa atcactttag aagatctgag agaagaattt ggcgatgacg
    28861 ttgctatgtt agtggacggc gtaaccaaat tagggaaaat aaaatataaa tcccacgaag
    28921 agcagctagc agaaaaccac cgaaagatgc tgctagcaat ggctcaagat ttacgagtta
    28981 tcatggtcaa attagctgat agactacata acatgcgtac gttgaagcac ctgcgagaag
    29041 ataagcaaag aaggatcgct caggaaactt tagaaatcta tgcgcctctt gcacatcgtc
    29101 tagggatcag ccggatcaaa tgggaattag aagatacagc acttcgttat ctaaatccaa
    29161 aacagtatta ccgtatcgtc cacttgatgc agacgaagag agaagaacgt gaaaaatacg
    29221 taagtggtac tgttgaagat attcgaatag cgacagaaga gctggggatt tttgcagaaa
    29281 tctatggacg gccaaaacac atttattcaa tctatcgtaa aatgaaagat cagaaaaagc
    29341 agttcaacga aatttatgac ttgctagcta ttcgagtgat cgtagattca atcaaggatt
    29401 gttatgctgt attaggagca atccacacaa aatggaaacc aatgcccggc agatttaagg
    29461 attatattgc catgccaaaa gctaatatgt accaatcttt gcatactacc gtgatcggtc
    29521 cggcaggaaa tccggtagaa attcaaatcc gaacacagga aatgcatgaa atcgctgaat
    29581 tcggggttgc tgcacactgg gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg
    29641 gtatgacgaa acaattaagc tggttccatg agatactcga acttcaagac gaaagctatg
    29701 atgcttctga atttatggaa ggcgtaaaag gagatatctt tagtgataaa gtctacgtct
    29761 tcacaccaaa aggagacgtt actgagttac caaaaggatc cggaccattg gactttgcat
    29821 acagtatcca tacagatatc ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg
    29881 tgcagcttga ttacaaattg aaaaacggag atatcattga gatcatgact tctccaaatt
    29941 catttggccc aagtcgcgac tggttgaaat tagttgctac tagcaaagca agaaataaga
    30001 tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt gatcaaaggc cacgaatccg
    30061 tggtcaaatg tattacagat ctaggattta cgcctaaaga tattttgacg aagaacaaac
    30121 tgcaagaagc actcgatcgt tttaattatc aaacagaaga tgatctctat gcagctgtag
    30181 ggtatggaga agttagcccc ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag
    30241 aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat catgaatcag ccgaaaaaag
    30301 aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact
    30361 tactaattcg tatcagtcgc tgctgtaatc cgattcctgg tgatgatatc gttggttata
    30421 tcactaaagg cagagggata tccattcatc gtcgagattg tccgaatgtt cagcctgaca
    30481 aaccaaatgt agcagaacgt ttgattgaag tcgaatggga agatacatcg aatacacgaa
    30541 aagagtatga tgcagatttg gaaatttacg gctataatcg ttcaggctta ttgaatgatg
    30601 tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag cgttgaagca cggacgaata
    30661 aagataaaat ggcgacgatc catttgacgg ttggtatcca gaatttatcc cacctaaaga
    30721 gtatcgtgga taaaatcaaa gcagtacctg atgtctacag tgtacgccgg acgaatggat
    30781 aggaggacag atatgagagc agttgttcaa cgggtaagta aagctgctgt gtcagtcgat
    30841 cagcaagaaa ttggaaaaat tggctacggc ctacttgttt tattaggcat tcatgaaacc
    30901 gatacgcaaa aagatgtgga ctatctggta aaaaaaattg cacaaatgcg tatttttgaa
    30961 gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg gaggacaaat tttaagtatc
    31021 tctcaattca cactgtttgc ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc
    31081 gcacgaccag aaacagctat tcctttatac gaatcattca atgaaggaat cagaagtcgc
    31141 ggcatcacag tcgctaccgg acaattcgga gcagatatgt cggtttctct cgtcaatgat
    31201 ggacctgtaa cgatcattat tgattctaaa aacaaataaa gtaatctggt tttcactagt
    31261 aaaaactatg aaacacgtcg cttaagttag actgataaac tctaacttaa gtggcttttt
    31321 tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa aagttaagct taaattatac
    31381 aagttatttt ttataggagg ttgctgaaga tgaaagaagt accagtagta gatatcaaaa
    31441 tcacagattt aaaaaaattg tatcaaaaag gatacaacat gttggaggag ttacgccatg
    31501 aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa agaagctatc acgatctacg
    31561 gatcatcagc agcaaaagta ttttatgatc caaggaattt caaacgaaaa ggtgctatgc
    31621 caaaattagt attgaagaca ctttttggtc aaggtggagt acagactttg gatggagctg
    31681 ctcatcacca tcggaaaaat atttttatgg atctaatgac accggaaaga atggaagatt
    31741 atcatcgtat cttagataag aatctgacac aagcacttga agcacagcat ggtcaatttg
    31801 aactatttga tttaagtaag atggttttct ttacatctat ttgcgagtgg gcaggtatca
    31861 atttatcagc gatttctaaa gatgaggtcg aaaaattagc agaatatcaa atctctatga
    31921 tcagcggaac gtttacttct ccgattgatc acataaaagg tgtcgagaat cgcaaaaaat
    31981 cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca aaatcctgtg gctggaaaag
    32041 aaaatgtggc attatatgct tttgccaatg ccacagattt agatggtcag cttttacctc
    32101 tggaagtggc agctgttgaa ttattgaaca tcatccgtcc aacggttgct ctaactgtgt
    32161 gggcggcatt gatgggacat gcacttttca gtagaccaga tttgtatcag caattaaaaa
    32221 atgacttttc gacgttacaa gatccgttta ttcaagaaat gagacgctat tatcctttct
    32281 tcccaatgct acctgcgatt tcgctgaaag aagtagaagt agatggttat cgtataccag
    32341 aaggcagttg ggttatttta gatttatatg gaacagacca tgatgaacga acagttgaag
    32401 cgccagattc ctttatgatc aaacggtatg tagggaaagc aaaagatatt tcctataaag
    32461 aagaatatga gatgattgca caaggcgggg gaaattttcg acaaatgcat cgctgtgcag
    32521 gagaatggat cactcttcat agtctacgtg tgttttccga tcagctagta aataagtttg
    32581 agttctctgt acctgaacaa gattggacga ttccattcaa tcagtttccg acttatccga
    32641 atagccgagc gctactttac aagaattaga ttagcataaa cttatcgaaa aaaaggaaga
    32701 tcaaacagtg ctgatcttcc ttttttcata ttactataaa ttgataaggt caattgatca
    32761 aaatccacga aatgcttcat caatggtttg ataatcttca ttggataaat gcaaatctaa
    32821 agcttggaca ttgtcagata cttgctctgg acgcttagct ccaggtataa ctacagacac
    32881 tcgtggattc ttcatgtacc aagctaaaac aagttgcgca ggagtggctt ggtatctttt
    32941 agcaatgggg cgaagtacat cgacagcggt taaaatttct ctgaaacgtt ctccttggaa
    33001 atctggattt ctttttcgag gatctccttc accaaattgt ttttcctcac caagttcata
    33061 ttttcctgtc agcaaaccgg aagcaagagg aaaataggga acaaaactga ttttgttttt
    33121 ttcaagataa gggaataatt ctttttcagc ctgccgatgg atcaaactgt atttatcttc
    33181 tactacatcg acatatccat ctgcattcgc ttctttcaat tgttctaacg tgaagtttga
    33241 cacaccgact gctcggattt ttcctgcttc ttttaattcg tgcaatgtag caactgattc
    33301 attttttggt gtgctttcat ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg
    33361 cagacgtttt agtgcatctt ccaccgattg tttgagaaac tcaggagaat tatcaaatgt
    33421 tcgcccttta tttggtacat gagcagcttt tgtagcgatg acgacacgtg agcgatcata
    33481 ttctttttcc ttcagtacct cgccaatcag ttcttcggaa cggccgtttc cgtaagcata
    33541 agcggtatcg atcaaattga tcccgctatt caaagctgta cgaactactt gttttcctgt
    33601 ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg gcatttgctc ctaatccaag
    33661 ctgttctgcg tagacttgtg aatgtccgat tcttatttct gccatagctg attcctcctt
    33721 tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa
    33781 tagtcagata tctcttgact ttgagactgg tttttagtag gttataagta atatcgaatg
    33841 tcaatgaaag agcgagtaag tttttgaaac tttacaagag aggatcatcg aggctgaaaa
    33901 tgatctaaag tttcattagc ctaaagacac tctggagatg gacgagtgaa aagccgtccc
    33961 gcgtacgagc gttattcgta agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt
    34021 atgaatttca ttcacccttg ttcatgatga acaagggtga tttttttatt tacatagcta
    34081 agaaagggag agaaaactat gagtttccaa agaccaaaag gaacaaatga tattttgcca
    34141 ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac gtttattatt cagagattat
    34201 cagtataatg aaatgcgtac accgattttt gaacactatg aagtgatatc ccgaagtgta
    34261 ggggatacaa cggatatcgt ttcaaaagag atgtatgatt tttatgacaa aggggaacgc
    34321 catgtgacgc ttcgaccaga aggtacagca ccaatcgttc gttcttttgt tgagcataaa
    34381 ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt atatgggacc aatgtttcgc
    34441 tacgaacgcc ctcaaaaagg acggttacgt caattccatc aaatcggagt agaagcattt
    34501 ggatcagaaa accctgcaac tgatgtggag agcatggtga tggcacttga tttcttcaaa
    34561 cagttgggga tcacgcaaat ccggctagtc atcaattcat taggtgataa agaaacgcgt
    34621 caagcctatc gtcaagcatt gatcgactat cttttacctt ttgaatcaga gctgagtgaa
    34681 gactctaaac gccgccttca tgaaaatcca ttacgcgtat tggacagtaa agacaaacga
    34741 gatcaaaaat ttgtggcaga agctccatct atcttagatt atctaagcga gcctgctcaa
    34801 aaacattttg ataccgttgt ttccatgctg gatgcattgg ctgttcctta tgaaatcgac
    34861 agtaatatgg ttcgcggact agattattac acacatacga tttttgaaat catgagtgac
    34921 gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg gacgttatga caatctagtg
    34981 gaagaactag gtggtccttc aacacctggc ttcggatttg caatgggtat cgagcgatta
    35041 ctcttgacta tggaggcaga ggaagtagtt atccctgcat ttaatgagtt ggatgcttac
    35101 gtggtcgcac tcggagacga aacaaatatc gaagcattga aagtggtcca agcgattcga
    35161 aactttggtt tttcagctga ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag
    35221 acagcagata aagcgaatgc caaattagta ttggtgatcg gtgaagatga gcttgctagt
    35281 caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa aaagttttcc attagaagag
    35341 atatatgaac attttgatga agtctatgat gaaatgactt tattcgagta ggaggaaaaa
    35401 aataaatggc acaaagaacg atctattgcg gattagtttc cgaacaacaa ttagaacaaa
    35461 cagttacatt aaaaggatgg gtacaaaaaa gacgagacct tgggggagta atctttatcg
    35521 acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag
    35581 catgggaaat cgcggataaa tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta
    35641 gaagacgcgg agaaaatgca gtaaatccta aaatggccac tggtgaattc gaagtgattg
    35701 caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc
    35761 aagcaatcag tgacgaaata cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa
    35821 tgactaaaaa catcaaatta agaaatgcga caacaaaagc gattcgtcat tatttagatg
    35881 ataatgactt tctcgatatc gaaacacctt acctagggaa atctacacca gaaggcgcaa
    35941 gagactacct agttccttca cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc
    36001 ctcaattgtt taaacaattg ctgatgaatg ctggattcga ccgttattat caaatcgttc
    36061 gttgtttccg cgatgaagat ttacgtggag accgtcagcc tgaatttacg caagtagata
    36121 ttgaaacaac gttcttaaca gcagaagaaa tccaaactta cacagaggga ttgattgcga
    36181 aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc atttcctcgt atgacctatg
    36241 atgaagcaat ggctcgctac ggcagcgaca aaccggatac acgttttgat atggaattga
    36301 tcgatctaag cgatacagtc aaagaagtag aattcaaagt cttccaaatg gctcttgaaa
    36361 acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag
    36421 acatggatca gttaggtcaa tatgtaggac aattcggagc taaggggctt gcatggctga
    36481 aagtagaaga agacggattg aaaggtccaa ttgctaaatt catgggagaa gcaacagaag
    36541 caatcattaa agcaacagat gcaaaaccag gtgacttgct gatgtttggc gcagataaaa
    36601 gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg tttaggaaaa gaattaggac
    36661 taattgatga atcgaaattc aatttcttat gggtaactga ttggccacaa tttgaatttt
    36721 cagaagaaga aggtcgatat gtctctgctc accatccatt cacgatgcca aaagaagaag
    36781 atatcccgtt attggctgaa gaccctgcaa aagtctatgc agaagcttat gatatcgtct
    36841 tgaacggtta tgaattaggc ggaggttcta tccgtattca tactcgtgag cttcaagaaa
    36901 aagtactaga aactctaggc tttacaaaag aacaaatgga agaacaattt ggtttcttgt
    36961 taacagcttt agattacgga ttccctccac atggcggtat tgcattagga ttggaccgtt
    37021 ttgcaatgtt attagccgga gaagataata ttcgtgaagt cattgccttt cctaaaaacg
    37081 gaaaagcagc agatgtgatg agtgatgcac caagtatggt ttcgccttta caattgttcg
    37141 aattgaatat tgatgtaacg aatatagaag aatagccgat aaaaaggtaa aaaggatttg
    37201 tcgaatacgc ttcttttgac aaccttgatt taattgaaaa acactttata atgaagccat
    37261 aacatataaa gcggggcttt tccatgaaaa aaacattcga aaattgggct tatcatgatt
    37321 acacgaccaa gctttcggta tctatcgttt atgcgttgat cgcatcagtc gcattgaatt
    37381 tcttttatca gccaggacat atttattcaa gtggtattac tggtctagct cagattttga
    37441 cgactttgtc aaaatcactg ataggattcg aagtaccggt atcaacaact ttgtatttac
    37501 tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg gaagaagttt acaattttca
    37561 cttttatcac agtcactttg acctcccttt gtatccatat tttaccggaa acagtcctat
    37621 ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt gatgggacta ggtattgggc
    37681 tagcgttgag aaacggccta tcatcgggcg gtctagatat cgtgagtatc acgattcgta
    37741 aaaaaacagg aaagtcgatt ggttctattt cgatctactt caatgcattg atcgtttttg
    37801 ttgccggaat tctatttggc tggcaatata tgttttacag tacgttgtct attttcgtca
    37861 gtggaaaagt aactgatgtc gtttatacta aacaaaagaa gatgcaggta atgatcgtaa
    37921 cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga
    37981 tcatcaatga agcagaaggt gcttataagc atgataagca gacagtattg atgacgatcg
    38041 ttacacgata cgaactgcct ctacttagag aagcaatgaa agcttctgat ccacaagctt
    38101 ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt ctatgaggat ggcatttaga
    38161 tcgagtttga caaaaattca tgaaatactg ctgtagacag acgttcgttt gtctgcagtt
    38221 tttttctacc atagtagaat cagcaaagaa aaactgcttt tttatttgaa attttcttaa
    38281 agttgaaaaa aagcatgttt atttttttaa atttatggga tgatagaaaa aaatagtaga
    38341 gaaaggacac gattctactt gctattttca cttaataccc gtatattacg aatgaaaaaa
    38401 gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac
    38461 atcactgggt ttgatgggct ccgttcatta gctgtggtgg gtgttatctt ctatcacctt
    38521 ctgccaacta gtatgaaagg cggctatttg ggcgttccga tttttttcgt cgtatctggt
    38581 tatttgatca cggatctgct gcggcaggaa tgggaacaaa atggaaaaat caatatttgg
    38641 caattttatg tcagaagaat gaagagactc tatccggcac ttgtttttat gcttgtgaca
    38701 gcttcggctt atatcacttt gtttcagcga gggctgttaa acaatcttcg aggagttgtg
    38761 gtcagcagct tactgtatgt caacaactgg tggcaaatca ataacggttt atcttatttc
    38821 gatcgatttg ctaatgagtc accgtttacg catatctggt ctttagcagt agaaggtcaa
    38881 aactatttgg tttggccgat cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa
    38941 tggatctttt atactgtgtt aggtacatcc ttcctctccg ccatcttgat gatgatcttg
    39001 tatacaccag gaggcgatcc aacccgtgtt tattacggta ccgacactcg tctgttttct
    39061 atatggttgg gaagtgcgct tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc
    39121 ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa tctcgctttt ggcactgatt
    39181 ctttgcttct tctttttaga tgatcattac aatttcatct attacggagg aatgctccta
    39241 gtgagtctgt tatgcgtagt gctagttgca gtgacagcac accccggagc aagtttgaat
    39301 cgatggctga caaatccagt atttacttgg attggcaaac gaagctatgg catttatctg
    39361 tatcagtttc cagttatgat tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt
    39421 ttgttgcata ctttagtgga aatcacattg attctaatca tcagtgaact ttcttatcga
    39481 tatgtggagc gtccgctagc acgttttgat tatggtcgta cattccaagt gatgaaagga
    39541 tggttcagca aaccgatcat cagtaagagc aaaccatggg ttgttccagg cacattactt
    39601 gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa
    39661 aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag cagaagaaac cagaaacaat
    39721 tcagaaaaca ccactagttc ggttgaccaa gcgatccttg ataagtacga gttgtccaag
    39781 gcacaaggga ataaggcaca aaaattagaa ctaacggcat ttggtgattc agttatgttg
    39841 gatgctgcaa cggatctaca agaactttat ccaaaagtag tggtagatgg agatgtcgga
    39901 cgacaattat atgccagtgt accgtacatt gaaaaattaa aggaacaaga tttgttgaaa
    39961 gacactgttc tgattggact aggaacgaat ggctcattct cggaagctca gtttgatagt
    40021 gtgatggatg caatcggtga tcgaaaagta tactgggtca atgtccgagt accgacgaag
    40081 cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga cgaaaaaata cgacaatatg
    40141 acgctgattg attggtacag ctacagtaat gaccatagtg actggtttta tgatgatcaa
    40201 gtccatccaa atcctgaagg gatgacacat tacattcact tagtcagcga agccttgcta
    40261 ggaggaagtt caagtacgaa cagcagtgag acaaacgtga cagaatgata gctacaaaaa
    40321 acttcaggca aaaacatttg tttttgcctg aagttttttt atatttaccg tgtttttaac
    40381 attcatatag attcaggaag caaaacggtt gagtgtgtct gtaaataacg ttctttttgt
    40441 tttcgggtga gttttttgaa tgcagcttct gcttttgttg cttcgcttct tgaagaaaat
    40501 acttctgcat ggatcatctt aacaggtctt cttttttgaa gacgagtata tttagcacca
    40561 gtaccattgt tatgttcctc taatcgtcta gaaagatcgg ttgtatatcc accataaaat
    40621 gtctgatctt tacagaataa tacgtaaaaa taatgttcat ttgccataga gcatctcacg
    40681 aatctctgca gtgtattctc catccatatt gtaagtgatc aatggtggag cgactttgaa
    40741 tccgtctaat ttgccttctt tgattccttc aactaacaaa atatttgcct cttttccagg
    40801 ttttgggtaa acaaattgga tccttttagg tgcaattcga tagtcttcca tgacctgaat
    40861 gatttctaag aaccgatcag gacgatggac catagtaaag cgtccatttg tttttagtag
    40921 cttacttgaa atatcgatca cttcttctaa cgtcgtatga atctcatgac gggcgattgc
    40981 caggtgttta ttagggttcg ttttatttgt tggcagccct ttgaaataag gtggattaca
    41041 gacgactaag tcacaagaat tatgtcgaac gacagataag ctgtctttca gatcgatggt
    41101 gtggacagtc atttgttcat ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc
    41161 tagacgaggt tgaagttcga tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag
    41221 tccaactgcg ccatttcctg cacaaaggtc aacaatcttc ccttttttag gtacacggga
    41281 aaagtgagca agcagcacag catctaaaga aaaagaaaac actttggaac tttggataat
    41341 cttgatatct tctgcgtata attggtcgat tcgttcgtct tcgtgaagca tggattccct
    41401 ccaaaatgaa aaaaatagtc gtctgtatta tactattttt attaaaaaga accaaggtgg
    41461 cttgtcaaac ctctattttt tatgcatact atgaccataa gaagggaaag gaagagacta
    41521 tgttttatcg atttatgcgc ggggtagttc gagtactatt agccttagtt aatggaaatg
    41581 cccgctatga gaataaagaa gttttaccgc aagatgagaa ttacatacta gttgctcccc
    41641 atcgtacttg gtgggaccca ttatatctgg cggtagctgc agcacccaag gaattcagct
    41701 ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg atttatatta actcgatcca
    41761 atgcttttcc agttaaaaga gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga
    41821 ttttgaaatc gacaaatttg agtttgatca tgtttcccag tgggacaaga catgctacag
    41881 aacttaaagg cggaatggca ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg
    41941 tatatcaagg tccgttgaca cttggggatc tgttcaagcg tcagagagtg acagtgcgtt
    42001 tcggcgaacc aatcgactta tcagatatca agaaaacgga taaagaaggt atggcggaga
    42061 tcgaacgacg aacacaagca gcttttgaca aattggacaa agaagtgaac cctgatttca
    42121 aatatgaagt caaaaaaaaa taaaatgata agtttatgcg tgggcggtct tttgaatcta
    42181 ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg agagacgtca agtctgccct
    42241 ctttttgcgt tccaatatgt aaaatagaag acaggtgtca tgtaatggat caaagtgtta
    42301 taataattag aaggacattt tttgaggtga ttgaatgcgt tttttacata cagccgattg
    42361 gcatatcgga aaaaaactac aaggttatga cttgattgaa gaccagcgtc atgtactatc
    42421 ttctatacta gatatcgcga aagaagaaaa agtagaggca atcgtgattg ccggagactt
    42481 atatgatagg agtgtcccag ctgttgatgc cgtagaatta tacaatgaat tgatggtcga
    42541 ttggaattta aaggaaaaga tcccgatttt tgctatatca ggaaatcacg atagcagtac
    42601 gagactttca gcgggtacgc catggttcag ttattcgaac tattttttgc gaacacagct
    42661 agcagaagca tttgaaccag tcgagtttaa aaacgtacaa tttttctttc ttccttactt
    42721 tgaaccaatt gcagcgagat tatattttga agatgacaat atccgaacga tcaagcaagc
    42781 ggtagccaag gtgatagata aaatgcagga gctatttgat tcagaaaaaa aacaagtatt
    42841 gattagccat ttttttgtaa tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt
    42901 aggcgggttg gattcgatcc ctggtgaact acttacaccg tttgattatg tagcactagg
    42961 acatttgcat aatcaagctg ctttgagaca ggataatgct cgctatagcg gttccccatt
    43021 gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac
    43081 aggcctggag ttgaatttta gggagatcga acccctgcat gatattgaag aaataacagc
    43141 gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc gtcaatcggg agaattacat
    43201 ctatatctat ctcactgata gaacgatcat cccgaatatg atgaaccagc ttcggcaaat
    43261 atatcctcgt attttgggga ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa
    43321 acgaaaagta ctagaaatga aagatcctca aaaattagca gaagcatttt ttagtgaaat
    43381 gacggaagag cagctaactg caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat
    43441 catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta caacttaaaa attttggtcc
    43501 ttttatcaat gagaccgtag atttttcacg tttgacagaa gcaccgcttt ttttgatcag
    43561 cgggaagaca ggagctggca aaacgacgat ttttgatggg atcaccttcg cactttttgg
    43621 tgaaacttcc ggaagacttc gctctggaaa agagatgcgt tctttgtttg ctacaccaga
    43681 cgaagaaaca agcgtgactt tttcttttga acatcagcag atgagctatg aaatcgaacg
    43741 aaaaccagaa caagttttag caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa
    43801 agtaacgttg accatttttg atgggaaagg aaaagaaata aaacaaatca caaaacgtac
    43861 agaagcagat cagttgatca gagaactgat gaatctcgat gcgaaacagt tttctcaaat
    43921 cgttttacta ccccaaggag aattccgtaa ttttttggtt tcatcaagca gtgataaaga
    43981 gactgtttta cgtcatttat tcggtactca atttttccaa cagttcaatg agcgattaaa
    44041 agaaaaagca aaacaacagc aacaaaaatt ggatcattta gagcaagagc tgcatcttct
    44101 gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca gcagggtttg aagccgtttt
    44161 agcccaatgg acaaaccatc aggcaatact cgaaaaacaa atcacggaag aaaaagaagc
    44221 tctaacggaa cttcaggaaa aacaaaagca actagaaaat tcctactatc gttttgaaag
    44281 cttgaaaaaa agttatgaag aaaaaacgat attaataaac aagcaaaagg aattagctgc
    44341 tcaaaaagac gaaatagagg aaaagaaacg atggattggg tattatgaat ttacgcaacg
    44401 attagtcgaa ccaatccagc gcctacaaga aataaaagtt gaaaaaatag aactgtcaca
    44461 aaaaagtagt gagcaaacaa agaaactagc gatgactacg cagaattatg aagagtggca
    44521 aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact
    44581 gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc
    44641 agaacaatta acaatttcat tggataaaga agaattgaat gccgagaagc tgtatcaaaa
    44701 tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa aatttactgg atcaggaaaa
    44761 actgcaagaa gatcgtcttg cttttggaca gttaaaatat ctagatgaaa agcagcaaga
    44821 aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca cagataagag aagaactcaa
    44881 aaaagcaact gaagaaataa caaaaaatag tcagctattg aaagaacaga cagaacaagc
    44941 agaacagctg aaaagccaat gggcaaagca gcaaattgct cgtcttcagc tttttttgcg
    45001 tccaggggag ccatgtcttg tttgtggatc aacggaacac cccatacaac aattaaaaca
    45061 cgaacagcca aataaggaag atatcttaaa agtagaacaa caactggcag aagctgagtc
    45121 tgctgtagaa agaactcagc aaatcattgc caaaagtgaa gcacatgaaa ataatcttca
    45181 acaacaatta tcagatttag aacaaaaaat catggcaacg aatatacaat tggctgaaca
    45241 agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct gagataacag aacaaaaaac
    45301 aaaaacaact cttgtagtaa tcgaacaatc tttagataga cgaaaaagca agatggccaa
    45361 tgaaagaata gagctggaag aaataagaca acaagcagaa aagctcgaac aacaattagc
    45421 tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc cagcaactgc aaggagaaat
    45481 caaaagttta gaacaacaat tgcaagatac ggatagtaac tgtttagaag aggagatacg
    45541 acgacttgaa aaacagttag aagaagactc gaatcaacta gctgttgagc agaaagcagg
    45601 agtgcaattg ttgcaagatc ttcatacgct tcggacacag cttgatcttc aacatgaaca
    45661 aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa agaatagcta aaaagttaga
    45721 aagccagtcg tatttttcaa caattgaaga aatctgtgca tttgcacaac agcaagcaca
    45781 atataaagaa aaccaacgta cagttacttc ttatcatgaa gagcaattag tgacagctga
    45841 ccggctgcgt caattagaaa atatccagct aactgagaca ttcccagata cagaagagat
    45901 cagccaacag ttagaagaaa taagacgtat gatcgaaata aagcaagaaa agatatacgg
    45961 tttacaagaa caaaaaatta gtaatcaaaa aatctacgat gaatgtcttt cgatttatca
    46021 cacaagccag acgaaattag atgagcttac gcaattacag cagctttcgc aaacattcaa
    46081 cggagaaaat cctaagaaga ctagtttgga acggtatgtg cttcaggttt atcttcaaga
    46141 agtgttacaa gtagcaaatg atcatctcca acgtttaacg aaaaatcgtt atcaatttga
    46201 gctagcggat accattggca gttatcgagg gaaaaccgga ttagaaatca atatctatga
    46261 tgatcaggca ggtatgtctc gaagtgcgca taccctgtct ggcggtgaga gttttattgc
    46321 agcactttct ttagcattag ctcttgccga tgtgatccag acacaagctg gtggtgtagc
    46381 aatcgatgca ttgtttattg atgaaggatt tgggtcatta gatgaagaag ctttggaaat
    46441 ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga atgatcggca tcattagtca
    46501 tgtaagagaa ttgaaagaac gagtattaca acaaataaga attgatacag aaggtagcgg
    46561 acaaagcaaa atacgctatc aacttggata aaaagggaga ttggtaacta tgaaatggag
    46621 tattccagaa aaagtcattg cacgtggacg tcaatacttg caagaaggcc gagtgttatc
    46681 ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta
    46741 tatggttgat ttggatggta cagcaaaaga gatagattac tgccaatgtc cctattggga
    46801 ggaacataaa tattgtaagc atacagttgc tgtggaactt tatttacggt cgaaaaatct
    46861 atctcgtatc atgaaaaaag accagccaat gctagaaccg gtaaaagcaa caagtgaggg
    46921 agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa aacaatcctc aagcggtgca
    46981 acctttgatt attgaatacc aagtagaaac gattgagact aatcagtatc acccagagct
    47041 ttctattttg gcactttact tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat
    47101 cgtaaaaaac atctatgatt ttttacaagc ttacacagaa aaagaaactt acactgttaa
    47161 taaacagtac cagtttcgtc tagataaacg ggcgtttcaa aaagaagatc aaaaaatact
    47221 cacgcatctt gcaggaagtg ctcagacgca tcaattatta ggtacgaacg gcttacaagt
    47281 caaaggaaaa ctagataaaa gatatctgct tctcccagta gaacaagccc gcttcctgtt
    47341 agagcagatg aatcagacta cacgtttgca tctgcagata aatgaacaaa acgatcaagg
    47401 aatacaattt tctgatcaaa acaaaccttt atcattcaaa gtggaaaaat ttggagaaga
    47461 ttatttgctg caagcattga atgattttga ttttttcttt atgcattatc agtggggagt
    47521 tatccaaggg acattttatt ctctgacgaa atatcaacaa acggtttatc aaacttggaa
    47581 acaattgatt cgccgattgg agaaaccaga agtagtgttc aagaaaaaag aattaccgtc
    47641 tttgtttaaa gagattatcc ctctgctgtc agagatcggc gacgtagcta ttgcccctgc
    47701 agtagaagag gaagtagcag atatccccgt cgaatttatt ttctttttcc gaaaagcaaa
    47761 aggaaaaatc caagctcgag tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc
    47821 gaagcatgag gtctcgacag aacaggcttt tcaggtgtta cgtgacacag tagaagagaa
    47881 acgagtactt gatctattca gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg
    47941 tccattgcca agcggggaag aactatatgc ctttttccgc acagaactat ctgtcttccg
    48001 tcagtttggt gaagtcagac ttggaagaaa attgcgagag ttgtatttag atgcgcataa
    48061 acatcaaccg aaaatcgaag taaccgagac cggttcatgg ctcgatattc gattcgatgt
    48121 gacagggata gaggagcaag aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc
    48181 tttttataca ttagaaaatg gtcaggttct ttcttttgat tcagaagaat ttcaacaaac
    48241 aagccaagtg ttgcaacaat tacgggaatc gatacgtaca gaagaaggaa cgatccatgt
    48301 acctaaaaat caaggattga tcattcaaaa tcaattggaa aaaagcaatg ctacttttag
    48361 tgaatcgttc caaacgatgg tacaagatct cattcatcca gaaagatacc aggcacagct
    48421 tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag caaggttttc gatggttgaa
    48481 gatgttaggt cattatcagt ttggcggtat tttagccgat gaaatgggac ttgggaaaac
    48541 actacagaca attgcctttt tactttcgga aaaagaagaa cggaaaagct tctctgcttt
    48601 gatcgtagcg cctgcgagct taatttacaa ctggcaggca gaggtgagaa aattcgcccc
    48661 ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa
    48721 agacacagat atccgagtga cttcttatgc cagcttaaga caggatctag ctaattatca
    48781 gtcgcaaaaa attgattatt tgattcttga tgaggcacaa atggtcaaaa atagcagtac
    48841 gaaaactgca caagcattga gagaattagc tgtacctcag cggtttgcac tgagtgggac
    48901 accaatcgag aataatttag aagaattgtg gtcattgttt gcaacgatta tgccaggatt
    48961 tttcccgact aaaacaaaat tcagggaact tgccgcagaa gaaattgcgc aaatgattcg
    49021 accgtttatt ttacggcgag ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga
    49081 gatgaatcta tattctgcac ttacagaaga acaaaaaact gtttatttag cttacttgcg
    49141 tcaaatgcgt gaagaaatca catcaatgga ttctgaagca ttcaagaaaa accggatcgg
    49201 gatacttgct ggtctgacaa gactgcgaca aatttgctgc gatcctcgtc tgtttattga
    49261 agattaccaa ggcggttccg gcaagctcga acaagtaaaa gatctactgg tagctgccaa
    49321 agagaacaaa cgaagagttt tgctgttttc tcaatttaca gggatgctga ctatcttgca
    49381 agaagagtta gccgagctag ggatctcgac attttattta agaggcagta cgaaaccaca
    49441 agatcggtta agtatggtag acgcatttaa tgctggcgaa aaagatgttt ttctgatttc
    49501 cttaaaggct gggggaactg gattgaatct cacgggagca gatacggtca tcttgtatga
    49561 cctttggtgg aatcctgcca ttgaagaaca agctgctgga cgtgcacatc ggatcggaca
    49621 aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga acgattgagg aacgcatgga
    49681 ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca
    49741 gttgacgaaa ctgactgaag aagacatccg catgatttta agtgtaggag aaatcgagga
    49801 gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg
    49861 tttatttgat ttaaaaaata gcaaggaggc tgggattcca ttttatccca gcttccttgc
    49921 tatcattttt ttatttctat ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac
    49981 agctgatcaa aagaagaaat ctcgatatcg caaattggag tctgttcaat tttgcgttct
    50041 cgatggttga accaaagcgc ttgccagtta gcactttttg cacctagaac atcattgtcg
    50101 taattatcgc caacgtataa ggtctcttct ggaagcatat gaaattcttt ctcagccagc
    50161 tgaaaaatct ctttttctgg tttttggaaa cctgttgctt gagaaatgag catattgcgg
    50221 gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc
    50281 ccatttgtga taattcctaa tgggacattc ttttccttca agaaatcgag ggtttttttg
    50341 acctcctcat gcaagcagat attatcaagt tcttcctcgt aaattttttg aaataagagg
    50401 ccatcttctt ccgtaatatg agggtagtct aaatctttta gtgattggct gatccgatgt
    50461 gctcgcatat attctagtgt ccaatctcct gccattactt ttgggaagtt ttcatcacta
    50521 tgatggcgga accggatata tagtggatgc atatctgcat ctgaaacaag cggaacgact
    50581 cttttgaccg cgtttcgaaa tggctgctgc tgatcataca tcgtatcatc aacatcaaac
    50641 acaactgatt taatcaaaat tttacacgcc tttcggtttt cattgatttt ttcacaaatc
    50701 cttgactatt gtaacagaaa aaattgtcta aaaaagctga taaataaagg tttttcttac
    50761 tatatttcta tttccttgac agaataaggt ttttatattt cagttttttt cactaaaaat
    50821 cgtgaaacac gtcaataagt ttgatgaaca aattaatcat tatggtagaa gtcgattgat
    50881 ttattttaat gcaatctctg tggaattttc gaaag
//
LOCUS       DANJJH010000006        49180 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000006 DANJJH010000000
VERSION     DANJJH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            119..931
                     /locus_tag="PWS53_000379"
     CDS             119..931
                     /locus_tag="PWS53_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL0870653.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            928..1728
                     /locus_tag="PWS53_000380"
     CDS             928..1728
                     /locus_tag="PWS53_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL0870654.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1889..3157
                     /gene="rseP"
                     /locus_tag="PWS53_000381"
     CDS             1889..3157
                     /gene="rseP"
                     /locus_tag="PWS53_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL0870655.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3225..4934
                     /locus_tag="PWS53_000382"
     CDS             3225..4934
                     /locus_tag="PWS53_000382"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL0870656.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5146..9504
                     /locus_tag="PWS53_000383"
     CDS             5146..9504
                     /locus_tag="PWS53_000383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL0870657.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9649..10122
                     /gene="rimP"
                     /locus_tag="PWS53_000384"
     CDS             9649..10122
                     /gene="rimP"
                     /locus_tag="PWS53_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL0870658.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10145..11320
                     /gene="nusA"
                     /locus_tag="PWS53_000385"
     CDS             10145..11320
                     /gene="nusA"
                     /locus_tag="PWS53_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL0870659.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11342..11635
                     /locus_tag="PWS53_000386"
     CDS             11342..11635
                     /locus_tag="PWS53_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL0870660.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11632..11943
                     /locus_tag="PWS53_000387"
     CDS             11632..11943
                     /locus_tag="PWS53_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL0870661.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11956..14262
                     /gene="infB"
                     /locus_tag="PWS53_000388"
     CDS             11956..14262
                     /gene="infB"
                     /locus_tag="PWS53_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL0870662.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14289..14636
                     /gene="rbfA"
                     /locus_tag="PWS53_000389"
     CDS             14289..14636
                     /gene="rbfA"
                     /locus_tag="PWS53_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL0870663.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14793..14966)
                     /locus_tag="PWS53_000390"
     CDS             complement(14793..14966)
                     /locus_tag="PWS53_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870664.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15414..17776
                     /locus_tag="PWS53_000391"
                     /pseudo
     CDS             15414..17776
                     /locus_tag="PWS53_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18144..19067
                     /gene="truB"
                     /locus_tag="PWS53_000392"
     CDS             18144..19067
                     /gene="truB"
                     /locus_tag="PWS53_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL0870665.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19071..20012
                     /gene="ribF"
                     /locus_tag="PWS53_000393"
     CDS             19071..20012
                     /gene="ribF"
                     /locus_tag="PWS53_000393"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL0870666.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20364..21149
                     /locus_tag="PWS53_000394"
     CDS             20364..21149
                     /locus_tag="PWS53_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0870667.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21162..22013
                     /locus_tag="PWS53_000395"
     CDS             21162..22013
                     /locus_tag="PWS53_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL0870668.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22137..22589)
                     /locus_tag="PWS53_000396"
     CDS             complement(22137..22589)
                     /locus_tag="PWS53_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870669.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23054..23422)
                     /locus_tag="PWS53_000397"
     CDS             complement(23054..23422)
                     /locus_tag="PWS53_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL0870670.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23572..24054
                     /locus_tag="PWS53_000398"
     CDS             23572..24054
                     /locus_tag="PWS53_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL0870671.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24091..25101
                     /locus_tag="PWS53_000399"
     CDS             24091..25101
                     /locus_tag="PWS53_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL0870672.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25216..25929)
                     /locus_tag="PWS53_000400"
     CDS             complement(25216..25929)
                     /locus_tag="PWS53_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870673.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26119..26709)
                     /locus_tag="PWS53_000401"
     CDS             complement(26119..26709)
                     /locus_tag="PWS53_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL0870674.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27193..27420
                     /locus_tag="PWS53_000402"
     CDS             27193..27420
                     /locus_tag="PWS53_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL0870675.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27436..28602
                     /locus_tag="PWS53_000403"
     CDS             27436..28602
                     /locus_tag="PWS53_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL0870676.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28836..29879
                     /gene="hrcA"
                     /locus_tag="PWS53_000404"
     CDS             28836..29879
                     /gene="hrcA"
                     /locus_tag="PWS53_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL0870677.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29973..30536
                     /gene="grpE"
                     /locus_tag="PWS53_000405"
     CDS             29973..30536
                     /gene="grpE"
                     /locus_tag="PWS53_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL0870678.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30587..32416
                     /gene="dnaK"
                     /locus_tag="PWS53_000406"
     CDS             30587..32416
                     /gene="dnaK"
                     /locus_tag="PWS53_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL0870679.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32567..33733
                     /gene="dnaJ"
                     /locus_tag="PWS53_000407"
     CDS             32567..33733
                     /gene="dnaJ"
                     /locus_tag="PWS53_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL0870680.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33815..34480)
                     /locus_tag="PWS53_000408"
     CDS             complement(33815..34480)
                     /locus_tag="PWS53_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870681.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34456..34629
                     /locus_tag="PWS53_000409"
     CDS             34456..34629
                     /locus_tag="PWS53_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870682.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34674..35411)
                     /locus_tag="PWS53_000410"
     CDS             complement(34674..35411)
                     /locus_tag="PWS53_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0870683.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35413..36915)
                     /locus_tag="PWS53_000411"
     CDS             complement(35413..36915)
                     /locus_tag="PWS53_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL0870684.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37174..39168
                     /gene="uvrB"
                     /locus_tag="PWS53_000412"
     CDS             37174..39168
                     /gene="uvrB"
                     /locus_tag="PWS53_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL0870685.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39178..41994
                     /gene="uvrA"
                     /locus_tag="PWS53_000413"
     CDS             39178..41994
                     /gene="uvrA"
                     /locus_tag="PWS53_000413"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL0870686.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42257..43522
                     /locus_tag="PWS53_000414"
     CDS             42257..43522
                     /locus_tag="PWS53_000414"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL0870687.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPQKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43526..44257
                     /locus_tag="PWS53_000415"
     CDS             43526..44257
                     /locus_tag="PWS53_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL0870688.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44392..45276
                     /gene="rapZ"
                     /locus_tag="PWS53_000416"
     CDS             44392..45276
                     /gene="rapZ"
                     /locus_tag="PWS53_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL0870689.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45273..46271
                     /locus_tag="PWS53_000417"
     CDS             45273..46271
                     /locus_tag="PWS53_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL0870690.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46305..47240
                     /gene="whiA"
                     /locus_tag="PWS53_000418"
     CDS             46305..47240
                     /gene="whiA"
                     /locus_tag="PWS53_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL0870691.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47783..48433
                     /locus_tag="PWS53_000419"
     CDS             47783..48433
                     /locus_tag="PWS53_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870692.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48620..>49180
                     /locus_tag="PWS53_000420"
     CDS             48620..>49180
                     /locus_tag="PWS53_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0870693.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16463 a         8422 c        10575 g        13720 t
ORIGIN      
        1 aaaaagaaaa aattattcag tttttaaata aaaaaacaat gatttcatag gaaaaacagt
       61 tagatattgc tataatactt aagatagtgg gaatctttta agtataggag gaaaaagaat
      121 gttacgtttt tttccgcaaa aaaacaaata tattcaagaa aaaagcgaat ttcaatttaa
      181 taccgatgga ccaattcctc agcacattgc catcatcatg gacggcaatg gtcgttgggc
      241 gcaaaaccgt cgtttgccac gagttgctgg ccataaagaa ggcatggaaa cagtaaagaa
      301 agtcacaaaa aaagcatcaa ggcttggagt gaaagtgttg acgctttatg ccttttcgac
      361 tgaaaactgg aagcgaccaa aagatgaagt aagttttctc atgcaactac ctgtcgactt
      421 ctttgatacg ttcgtaccag aattgattaa agaaaatgtc aaagttcatg tcatgggtta
      481 tgaaaacgta ttgccagaac atacgcaaga tgctgtaaga cgtgcaattg agcaaacaaa
      541 aaacaatact ggaatggtat tgaattttgc tttgaattat ggaagcagag cagagatcgt
      601 gactgctgtc caagaaattg ccgaagaagt agcaaaagga gaaatacacg cagaagaaat
      661 cgatgatgaa ttgattgcta agcatttaat gacaggtttc ttgcctaaag agttacaaga
      721 tcctgagctg atgatccgta caagtggaga agagcgaatt agtaattttc tgttatggca
      781 gatcgcatat agtgagctgt attttacaaa agcgctttgg cctgattttg atggtgcgca
      841 tcttgaagaa gcaatcgcct cttatcaaaa tcgagaccgt cgattcggcg gtgtaaaaaa
      901 aacagaagat gagaaagggg atcagtcatg aaacaacgtg tggtcacagc agccgtggcg
      961 ctagtcttgt ttattccgat tatatggatc ggaggcatag cggttgagct tacagcagca
     1021 atcttagcag taataggagt ttacgagctg tttcggatga aaggattgac actcttaagt
     1081 tttgaaggag ttttatccgc cgttggtgca gtgttacttg ttttgccaaa agaaagatgg
     1141 tttttctttc ttccagagaa tgtaagtaca tttatgttat tttatcttat agttatgatt
     1201 cttttaggaa catcagttat ttctaaaaat acgtatacga ttgatgaagc aggttttcct
     1261 gtgcttgtca gcttgtatgt gggaataggt ttccagaatt ttgtcaatgc acgaacagaa
     1321 agcttggctg ttttgatatt tggtctattt attgtttggg caactgatat cggagcatat
     1381 atggttggtc gacgttatgg tcagcataag ttaagtcctg atatttcgcc caacaaaacg
     1441 attgaaggtg cgctcggtgg tattggtagt gctgttgtag tcgcatttct ttatttattg
     1501 ctatacccag caaaagaact atttaatcat ggtatgtttg tcatgcttct gatgacgatt
     1561 attttttcag cagtcggaca atttggtgat ttggtcgaat ctgcaatcaa acgtcattat
     1621 gatgtgaaag actcgggaaa tatcttacca ggccatggtg gaatcttaga ccgtttcgac
     1681 agtttgctgt ttgtatttcc tatcatgcat ttatttggca ttatttagta agtatacctg
     1741 cgtttctttg tgaatttcat aaaagacgca ggtttttatt tttcattata ttgatactat
     1801 tggtgtcttt ggtatactat ggtaaaatta tagtgtttta tcgttgtttt ataaaaaaca
     1861 caaaaaacaa gtaaaggaga aaaaagctat gaaaacgatt ctgacattta tcatcgtttt
     1921 tggtatatta gtgattgttc atgagtttgg tcatttcttc tttgcgaaac gatcaggaat
     1981 cctcgttcgc gaatttgcta tcggtatggg tccgaaaatt tatgggcatc aagcaaaaga
     2041 cgggacaaca tatacgcttc gcctattacc tattggcggt tatgttcgaa tggcaggcaa
     2101 tggggatgat gaaacagaaa tggcaccagg aatgcctctt tctcttttac taaattctga
     2161 cggcatcgta gagaagataa atttaagtaa gaaaatccaa ttgacaaatg ctatcccgat
     2221 ggaattgagc cgttatgatt tagaagatga gctgactatc actggttatg tgaatggaga
     2281 cgaaacagaa gtagtaacct accctgttga tcacgatgcg acgattattg aaaacgacgg
     2341 aacagaaatc agaattgcgc caaaagacgt acagttccaa tcggctaaat tgtggcagcg
     2401 tatgctgacc aactttgctg gacccatgaa taactttatt ttggcaatcc tgttattcat
     2461 cattctggca tttatgcaag gtggtgttca agtgacgaac actaatcgcg taggagagat
     2521 catgcctaat ggagctgcgg cagaagcagg cttaaaagaa aatgatgagg tagtcagtgt
     2581 ggacggaaaa gaaatccatt catggaatga tctgacaaca gtcattacga aaaatccgga
     2641 taaaacatta gactttaaga tcgaacgaga aggtcaggtg caatctgtcg acgtgacacc
     2701 gaaatctgta gagtcaaatg gcgaaaaagt tggccaactt ggtataaaag ccccaatgaa
     2761 tacaggtttt atggataaga tcattggagg gacgcgtcaa gctttcagcg gttcattgga
     2821 aattttcaaa gccttaggat cactatttac aggtttcagt ttagataaat taggcggacc
     2881 tgtcatgatg taccaattgt cttcagaagc agcaaatcaa ggtgtaacaa ctgttattag
     2941 tttgatggcg cttctttcaa tgaatctcgg aatcgtcaat ctgcttccga ttcctgcctt
     3001 agatggcggg aaattagtct tgaatatttt tgaagggatt cgcgggaaac cattaagtca
     3061 agaaaaagaa ggaatattga cattagctgg ctttggattt ttaatgttgt tgatggtgct
     3121 agttacatgg aacgatattc aacgattctt tttctagaaa aagttgacaa atagggtata
     3181 ataacaaacg ataactattt tgaggaaata aaggagtata tagaatgaga caatcaaaaa
     3241 tgttgattcc aacattacgc gaagttccaa atgatgcaga agtattaagc catcaaatct
     3301 tgctaagagc tggatatatc cgccaagtat cagcgggtat ttattcatat ctgccattag
     3361 caaatcgtgt attggaaaaa ctgaaacgca ttatgcggga agaatttgaa aaaatcggag
     3421 cggtagaaat gctgatgcct gccatcttgc cagcagaact ttgggaagaa tccgggcgtt
     3481 acgaaacata tggaccaaac ttgtatcgtt tcaaagaccg caatgatcga aaaatgatcc
     3541 ttggaccaac tcacgaagaa acttttactg atttgatgcg taatgaaatc aattcatata
     3601 agaaattacc tttaaatctt tatcaaatcc aacctaaata tcgtgacgaa aaacgacctc
     3661 gcttcggttt gcttcgcgga agagagttta tcatgaaaga tgcgtattct ttccatgcaa
     3721 cggaagaaag tttggacgag acatataaag attacgaaaa agcctacacc gaagtattca
     3781 agcgttgtgg cttagaattt cgctcgatta tcggagacgg tggcgcaatg ggcgggaaag
     3841 actcaaaaga attcatggcg atctcagaaa tcggggagga tacgatctgt tattcgactg
     3901 aaagtgatta tgctgcaaac ttggagatgg ctacaagcta ctatgtatca aaaaaatcgc
     3961 atgaaacaca gcttgaattg gagaaagtag ctacacctaa cgtaaaatca attgacgaag
     4021 tagcagattt cttttctgtt gaacctcaaa aaatcatcaa gtctgtattg ttcattgcgg
     4081 atgaacaacc tgtccttgtt ttggtacgcg gagaccatga agtcaatgac gtaaaactga
     4141 aaaacttttt atctgctgac tttttagaag aagcaacaga agaagaagca caaaaatatc
     4201 taggtgctga gtttggttct gtcggaccag tcggtgtttc agatgacgta aaagtatatg
     4261 cagaccgtta tgtccaagat ttagcgaatg ccattactgg agcgaacgaa acaggattcc
     4321 actataaaaa tgtgaatcca gaacgcgatt tcactgtact ttcttatgaa gatcttcgtt
     4381 ttgtacaaga aggagatcct tcaccagaca acaatggtgt tcttgccttt actcgaggaa
     4441 tcgaaatcgg acatatcttc aaaatcggca ctcgttacag tgaatctatg ggagcaactg
     4501 tactagacga aaatggacga gaaaaatttg tgattatggg ctgttatggt atcggtgtaa
     4561 gtcgtttatt atcagcaatc gtagaacaac attcagatga acaaggaatc aactggccaa
     4621 aaggaattgc gccatttgat ttgcatatcg tccaaatgaa tctaaaagac gaataccaaa
     4681 caaacctgac gaatgaacta gaagaagcga tgacaaaagc tggctatgaa gtactggttg
     4741 atgacagaaa tgaacgcgca ggagtgaaat tcgcggattc tgatttgatt ggctgtccaa
     4801 tcagaatcac tgtcggtaaa aaagcagtgg atggaatcgt agaagtgaaa atcaagaaaa
     4861 ctggcgaaat ggttgaagta cgtaaagaag aattggtgaa tactttacca atcctcttga
     4921 accaaggcga ataataagcg ttaaaacaca gaaaataaaa atagctgaat cagctgtttt
     4981 tgacaatctg atgattcgct tttaaaattg gaactgcgat ttgaatcgca gttccaattt
     5041 tttaatgtaa aactcagaaa aaacgagtat actaaaaggt actgaaaaag aagaggataa
     5101 gatgcgatca attgcattgt ttctcatcag aaagggcgta tatctttgtc agaaaaacga
     5161 gatttatttg aaaaactgtt ggaacaaatc caactagagg aagcagaaaa aaatcatcca
     5221 ctgcttactg ctggtgaaat ggatagtgta gtggtccatc gtaaatcgcg tttatgggaa
     5281 tttacgctgc atttttccaa aattttgcct attatgctat atcggtcatt gactcagcat
     5341 ctgactatcg cttttaaaga tattgcacaa gtcaaactga atatcttagc agatgaccag
     5401 acattcgatg aacagctctt gcaagattac tgggcccaag ctttggaaaa tcagcaatgc
     5461 gatacaccac tagcacagca agtattgaaa acgcaggtac ctgtgataaa agatcgtaaa
     5521 gtcatcttgc caatcgacag tacaggagct atttcttatt tgaagcaaca atacttgcca
     5581 ttagtagaag agttgttcgt cagttacgga ttcccaaaat ttcgtatcga accagaagtg
     5641 gatgagcagc aagcggaacg agtattgaaa ctttttgaag aacgcaaaca agaacaggca
     5701 gaagccttta tgaagcaagc agctgaaagt ctagttgtcc acgaacaaaa gaaaaaggaa
     5761 agaaaagagc aatcgccagc tcttgaagga cccatccaca tccagctagg acgaaatatt
     5821 ccagccgatg aaccaacgac accaatgatc agtatcgttg aagaagaacg aagagtcaca
     5881 cttgaggggt acgtattcga caaagaagtc agagaactac gttcaaaacg caagatcttg
     5941 actttgaaaa tcacagatta tacttcatca tttatcgtca aaaaattctc gaacggcgaa
     6001 aaagatgaac aagtatttga tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt
     6061 attcaagaag atacgtttgt ccgtgatttg gtcatgaatg cgcaagatat catagaagtc
     6121 aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt
     6181 catagcaata tgagtacgat ggatgcaaca aacagcatct ctgatcttgt ggcacaagca
     6241 gggaaatggg gacaccgagc tatcgccatt acagaccacg gtggtgcgca agcctttccg
     6301 gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat
     6361 gtcgtagatg atggtgtccc aattgcttat aatgatgcac atgaagcatt gagtgaagcc
     6421 acatatgtcg tatttgacgt ggaaacgaca ggattgtctg cagtatatga cacgatcatc
     6481 gagctagccg cagtcaaaat gtataaagga aatgtgatcg aaagctttga tgaatttatc
     6541 gatccaggac atcctttgtc acgaacaacg gtagatctga ctggtatcac ggatggaatg
     6601 gttcgaggat caaagtcgga agaagaagta ttgcggatgt tccttgaatt ttcaaaagat
     6661 acaattttgg tagcccacaa cgcggctttt gatatggggt tcttgaatac tagctacgca
     6721 aggtatggta ttcctgaagc ggccaatcca gtcattgata cgttggaatt agctagatat
     6781 ctgtatcctc aattcaaacg tttcggatta ggtgtgttat ctaaaaaatt tggtgtcagc
     6841 ttggaacagc atcaccgagc aatctatgat gctgaagcaa ctggtcactt agcatggatc
     6901 tttgtgaaag aggcaatgga caatcataac atgctttatc atgatcagct aaatgagcat
     6961 atcggagaag gagattctta caaaagggct cgacctttcc atgtaacgat tttagcaaaa
     7021 aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc
     7081 gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc
     7141 ggctcagctt gtgataaggg agaaatattc gaagccatga tgcaaaaagg ggtagaagag
     7201 gcaagaaacc gagcaaaatt ctacgattat attgaagtta tgccaaaagc ggtctatgcg
     7261 cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat
     7321 ttggtagaaa tcggaaaaag tttggataaa atcgttgttg caacaggtaa tgtacattat
     7381 ctgaacgaag aagatgccat ttaccgaaaa attttgatca attctatggg cggagcgaat
     7441 ccgttgaatc gtcatagttt gccggatgtc catttccgaa caaccgatga gatgctaact
     7501 gcatttcact ttttagggga agaaacagcg aaagagatcg tagtagaaaa tacgaataaa
     7561 atcgcagata tctgcgaaga agtgattcca gtcaaagatg aactttatac accgaagatt
     7621 ccaggttcag aagatgaaat atcagaattg agttacacaa aagcaaaaca aatgtacgga
     7681 gatccgttac ctgagatcat tcaaaaacga ttaaaaaaag agttgaattc tattaatggt
     7741 aatggcttct cggttattta tctgatcgcc caaaaactag tgcataaaag taatgaagat
     7801 ggctatctag taggttcacg tggctctgtt ggctctagct ttgttgcgac gatgacagga
     7861 atcactgaag ttaatccgtt agcccctcat tattattgtc cagagtgcca atattccgag
     7921 ttttttgaag atggtacgta tggttctgga tttgacatgc cagaaaaaca atgtccaaaa
     7981 tgtggtgcac gcttaaacaa agacggtcat gatattccat ttgagacatt tttaggattc
     8041 catggagaca aagtgcccga tattgatttg aacttctcag gtgattatca agcagaagcc
     8101 cataactata cgaaagtatt gtttggcgaa gactatgttt accgtgccgg aacgattggt
     8161 acagtcgccg acaaaacagc atatggctat gtaaaaggat acgaaaggga caataatctg
     8221 cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg ctactggagt caaacggaca
     8281 accggccagc atccaggggg gatcatcgtt attcccgatt atatggatgt ctatgatttt
     8341 acgccgatcc aatatccagc agacgatcaa aattcggaat ggaaaacgac tcattttgat
     8401 tttcactcga ttcacgataa tgtattgaaa ttagatattc tagggcatga tgacccaact
     8461 gttattcgga tgcttcaaga tttatcaggg atcgatccac aaacgatccc tacggatgac
     8521 ccggaagtga tgcggatatt tgccggtcct gaagtacttg gagtaagtca ggaacaaatt
     8581 tattccaaaa cagggacatt aggtattcca gaattcggca ctcgttttgt tcgagggatg
     8641 ctagaagaaa cgcatcctac aacttttgca gaactactgc aaatttccgg actatctcat
     8701 ggtacagacg tatggttagg aaatgcggaa gaattgattc gacgaggaga tgcgacactg
     8761 gcggaagtaa tcggctgtcg tgatgatatt atggtttatt tgatccatgc tggattagat
     8821 agcgggatgg catttaaaat catggaaact gttcgtaaag gacaatggaa caagatccct
     8881 gatgaattgc gagaaaccta tctatcggcg atgaaagaaa ataatgtgcc cgactggtat
     8941 atcgattctt gttcaaaaat caaatatatg tttccgaaag cccatgctgc agcttacgta
     9001 ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc ctattttgta ttactgtgca
     9061 tacttttctg tacgtgcaga cgactttgat ctagtctcca tgtgcaaagg aaaagatgct
     9121 gtgaaacaag caatgaaaga aatcacggac aaaggattag atgcttccgt aaaagaaaag
     9181 aatcaactga cagtgctgga gttagccaat gaaatgctgg aacgaggatt caaatttgga
     9241 atgattgatt tgtataaatc ggatgccgtt aactttgtta ttgaaggtga tacgttgatc
     9301 gcaccattta gagctgtccc aagtttggga accaacgtgg caaaacaaat cgttgaagca
     9361 agaaaagacg gtccattttt atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa
     9421 acattgatcg aatatatgaa cgacaatggc gtattgaaag atctaccaga tgaaaatcaa
     9481 ttatcgttat tcgatatgct ataagcatct gttttcctgt ctgcttgtaa ttttaagcag
     9541 agtatggtat agttcttata ggtaatgata ctaactgtga gtgagcggat ttttcgctca
     9601 ctctttttaa tggaattacg cacttaattt tttaaggagg cgaaactttt gagtagcgtc
     9661 gttgaaacag tcacagaaat ggtgacaccg attttagatg aacaaaattt tgaacttgtc
     9721 gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac gagtatttat tgacaaagaa
     9781 ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga aactgagtga aaaacttgat
     9841 gcagcagatc cagatccgat tcctcaagct tatttcctgg aggtatcctc cccgggagca
     9901 gagcggccat tgaaaaaaga aagcgactat gaaaaggcac ttggtgatta tgtccatgtc
     9961 tccttatacc aaccagtaga tggcgaaaaa caatatgaag gatttcttca atcatttgat
    10021 gctgaacagc ttacattgaa aatacgcata aaaacacgag aaaaagaatt gacttttgac
    10081 cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt aatcacggag aggaatcaga
    10141 aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta gaagctgaaa aagggatctc
    10201 aaaagaaatc gtgatcgatg ctttagaagc agcattggtt tctgcataca aacgtcacta
    10261 tggccaagca caaaacgtcg aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta
    10321 tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag ttggaagtct cattgaaaga
    10381 tgcactttta atcaatccag catatgagat cggtgatacg atccgttttg aagtaacacc
    10441 aaaagatttt gggcggatcg ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg
    10501 tgaagcagaa agaacgatta tttataacga attcagtgct tatgaaaaag acatcatgca
    10561 aggaatcgtc gaacgccagg ataaacgtta tatttatgtc aatcttggta aaattgaggc
    10621 agttctttct aaacaagatc aaatgccaaa tgaattttat caaccacatg atcgaatcaa
    10681 agtttatgtt tcccgagtag aaaatacgtc taaaggtccg caagtatttg ttagccgcag
    10741 ccatccagac ctattgcgcc gcttatttga gcaagaagta ccagaagtct atgatggaat
    10801 cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc
    10861 aaatgatcca aatatcgatg cagtcggtac atgtgtcggg ccaaagggac aacgagttca
    10921 agcaatcgtc aatgaattaa aaggcgaaaa catggatatc gtcgaatgga atgaagatcc
    10981 agctgtattc attgcaaatg cattgaatcc tgcacaagtc atggatgtga tcttcgatga
    11041 gacaaatcca aaggcctgca cagttgtcgt tccagactac caattatcgc tagcaatcgg
    11101 taaacgcgga caaaacgcac gtttagctgc taaattgact aaccataaaa tcgatatcaa
    11161 atcagaatct gacatgacgg aattttatga aaatcaagaa caacaaaaag aaacagaaga
    11221 actgcatgat gaagcaatcg ttcagtcaga tttaacagac gatgaatatg aaacaattgc
    11281 attcaacaat gaaacggtcg aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga
    11341 gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa
    11401 aaaagagatg gtccgaatca cccgttcaaa agaaggggaa gtatcgatcg atccaactgg
    11461 aaaaatgcct ggacgaggag cgtatgtctc acttgagccg gaagaagtac aacaagcttg
    11521 ggataaaaaa attctcgatc gagtactcga aacaacatta acagatgagt tttatcaaga
    11581 acttcttgac tatgtcactc accaaaaagc acgtaaagag ttatttggaa aatgaataca
    11641 gttaatcggc aaaaagccat gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc
    11701 acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg
    11761 gcaaccgatg caagcgagaa tacaagaaaa aagataaaag ataagagttc atattatgaa
    11821 gttccttgct ttgagctatt ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc
    11881 aaggtattcg gaataatgga tgccggattt gcgaagaaga ccaaggagct aattgaaggt
    11941 taggaaggtg attgcatggg caacaaaaga atctatgaat tagcaaaaga actcaaaaaa
    12001 tctagtaaag acgtcgtgga aaaagcacaa caacttggca tcgatgtgaa aaaccatatg
    12061 ggtgcgatta ctagcaatga tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg
    12121 aaacaacaag ctgcacaaca agcaagcaga aaaccagcca atcaacagcc gaacgatcaa
    12181 cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa ataaccgcag ttatcaagat
    12241 cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat
    12301 acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt
    12361 actaacaata acaacaaacc aaacaataac aatcgcggca aatttaataa taataaccgt
    12421 aatcgttata ataaaaaagg taaaaaaggc aaacagcaac aatcaactaa accagctgta
    12481 ccaccacgca aattccgtga attgccagat gtattagagt atactgaagg tatgaacgta
    12541 gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa tcattaaaaa attatttatg
    12601 cttggtgtca tggtcaatca aaaccaagca ctggataaag atacgattga actgttagcg
    12661 acggactatg gcatggaacc acaagaaaaa gtccaagttg acattgctga tatcgataaa
    12721 ttcttcgagg cagaagaagt cgatccagat aaattagtct cacgtccgcc agtcgtaacg
    12781 attatgggac acgtagacca tgggaaaaca actttattgg atacattacg ccattcacgt
    12841 gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg gtgcctacca gatcgatatt
    12901 gatggtaaac caatcacttt cttggataca ccaggacatg cggcctttac aagtatgcgt
    12961 gcacgtggag caagtatcac agatatcacg atcttagttg ttgctgcaga tgatggcgtc
    13021 atgccacaaa cagttgaagc aatcaaccat gccaaagctg caggcgttcc aatcatcgtc
    13081 gctgtaaata aaattgataa accaggtgca aaccctcaac atgttatgca agaattaagt
    13141 gagtatgagt taattcctga agcatggggt ggagaaacga tctttgttga gatctcagcg
    13201 aaattcggcc aaaacatcga agaattgctg gaaatgatcc tgttagtcgc agaagtagaa
    13261 gatttaaaag cagatccaac acaacgcgca atcggaacag tgatcgaagc acgtcttgat
    13321 aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag gaacacttca tgttggtgac
    13381 ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt
    13441 cgcgataaag aagcaggacc tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct
    13501 caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa
    13561 gaacgcgcga aacgtgcatt attagaaaac cgtgctgcca caagccgtgt gactttggat
    13621 aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca
    13681 gatgtacaag gttcagcaga agcactggct gcatcattga agaagattga tgtggaaggt
    13741 gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca atgaaagtga tgtaacctta
    13801 gcagcagcca gcaacgctat tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa
    13861 gtgcaagctg atactgaaga agtcgatatt cgtcttcacc gtatcatcta taaagccatc
    13921 gaagaaatcg aaactgcgat gaaagggatg ctagaccctg aatttgaaga aaaaatcacc
    13981 ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc
    14041 tatgtaacag aaggatatat tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc
    14101 gtaatctatg aaggccaact tgcaagtctg aaacgattca aagatgacgt gaaagaagtc
    14161 aaaatgggtt atgaatgtgg tgcaatgatt gaaaaattca atgacatcaa agtagatgac
    14221 gtaattgaag gatttatcat ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc
    14281 caatcattat ggctaactac cgtgaccgcc gagtcggtca agaaatctta aaagaagtca
    14341 atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg
    14401 tgaaagttac gggggactta cagcaagcaa cgatctatta cagcatttta tctgacttag
    14461 catcagataa gaaaacagca caagatggat tgaataaagc atcaggattg attcgtcgag
    14521 agttagggca caatctaagt atttataaga cacctgaatt gttctttgaa cttgatgaat
    14581 cagttgtcta tggcaaccat atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa
    14641 aaactcccgc aaatcctgtg aaatcagggg ttgcgggatt tttttgtttt cattaagacg
    14701 tattacctaa gagttccatg actttagtct tgcggcgtgt cagctaacca aaaaagagaa
    14761 aaagaaaagt aaaaaattcg actttctttt ttctagtagt atataaattt ttgcatatag
    14821 ataaagatca agataccgat aacggaagct agattgtaat ctattttagt gacagtcagg
    14881 tatagccaag taactagaag atcagtggaa cctgcaccat aaaagtttga gtttgtcggt
    14941 ccgttgacag ttaaaagaaa aatcacgttg aagttattga tatttccgat tacgccgctt
    15001 cgatttggtc acgatccaaa ttttggataa tccctgtaga aattagcata gtagctggga
    15061 taccaattca catattgaca aagataacag agacttttac ataaaacgga tcaatcagga
    15121 aaggaattgc tcgatccagc cccagttttc cagcaatgtg ttaactggcc cagcgtcatt
    15181 cagcaaatta tgcatgatga ggagcgaaat gaactgcgga acagccattg tgataacgac
    15241 aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata
    15301 cctgttcatt tggctagata taaaatccaa acaaatgaac agaagtgctt attaggtttc
    15361 aattgggagt atctgcgcta gaataagagt aataatgaaa aggagtgtta tgtatggatt
    15421 attctagtaa agaatacttt gataaaatga cggcttggtg gcgtgctgct aattatcttt
    15481 ctgttgggca actttatctt aaagataacc ccttactgag acgtacactg aaacccgaag
    15541 atgtcaaaaa acatccaatc ggtcattggg gaacgattcc tggccaaaat tttatttatg
    15601 tccatttgaa ccgagtcatc aataaatatg atttgaacat gttttatatt gaagggccag
    15661 gacacggcgg acaagtcatg gtatcgaatg cttatttaga tggaagttac acagagatct
    15721 atccagaagt aacggaagat gagacaggaa tgcaaaaatt attcaaacga ttttcatttc
    15781 ctggaggcat tgcttctcat gcagcaccag aaacaccagg ttctatccat gaaggcggag
    15841 agcttggata ctctctttca catgcagtcg gagctgtatt agacaatcct gaagtaatct
    15901 ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc tttagctgga agctggttct
    15961 caaatgtctt tatcaatcca gtgatagatg gagctgtttt accgatcctt catttaaatg
    16021 gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc agatggagaa ttagcaaatt
    16081 actttaacgg acttggctgg gaacctttct ttattgaagg aaatgattca gaaaaattaa
    16141 atcctgttat ggctgaaaaa atggatcaag caatcgagaa aattaaatcc atccaaaaag
    16201 aagctcgcct gaagacagca acagatgtcg tgatgccaaa atggccagta ttgatcgtac
    16261 gcacaccaaa aggatggacc ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct
    16321 tccgagcaca ccaagtaccg attcctgtag atcaagaaca tatggatcat gcagatgctt
    16381 tactccgttg gctgaaatcc tatgaaccag aaaaattatt tgatgcacaa ggacgtattt
    16441 tagaagaaat cagagaaatc gcgcctactg gtgaccaaag aatggcaaaa aatcctatca
    16501 ccaatggtgg aatcgatcca agaccgctta ttatgccaga ttggaaaaaa tatactttgc
    16561 aatttgaaaa accaggctcc gttaaagcag aagatatgac cgaacttggt aaattcgttc
    16621 gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt tggaccagat gaaacaaaat
    16681 caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca atggatggaa aaaatcgaac
    16741 cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac
    16801 accaagatga agggttctta gaagggtacg ttctgactgg acggcatggt tttttgcaag
    16861 ttacgaatca ttcttgcgag tggttgactc tatgttgact cagcacttca aatggatgcg
    16921 gaaatcacat gacctttctt ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc
    16981 aacagtcttc cagcaagatc acaatggata tagccaccaa gatccaggaa tattgacaca
    17041 tttggcagaa aagaaggcgg agttcatccg tgaatatctg cctgcggatg caaatacatt
    17101 gctcgcagtc atggataaag cattccgctc gtcagaaaaa atcaatttga tcatctcttc
    17161 caaacatccg cgtgctcaat tttactcagc agaagaagca gccgtcttag tgaatgaagg
    17221 cttgaaaatc attgattggg cttcgacagc aaaagaggag gaacctgaat tggtcattgc
    17281 tgctgcagga acggaatcta acttagaagc attagcagct gtaactttgc tgctggaaga
    17341 attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc
    17401 aagtcaagat ccgcgtgggt tgagcgatga agaatttgat caatatttta caaaagacaa
    17461 accgatctta tttgctttcc acggttatga aacattggtc cgcacgattt tctttgatcg
    17521 tcataaccat catttaatga ttcatggcta caaagaaaat ggagatatca cgacaccatt
    17581 tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg gcaaaagacg cagctttaaa
    17641 aatcaaagga agtcaagcag aagattttgc gaaaaaaatg gatcagaaat tacaagaaca
    17701 tcaaaactat attcgtgaaa atggtattga tctgccagaa gtattggatt ggaaatggaa
    17761 aaatcttgac caataaaatg ataaaatcga tcccgcaaaa tagaattttt tacattgatt
    17821 aagaaactga aaaaataaat aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt
    17881 tttttcgttt ttgggaagtg aaaaccaaac tatttacctg taagccaccc ataaatccaa
    17941 tcaaatggtg caaagaaaaa atcttccatt ctattccctc ctttaaaaaa gcataacatg
    18001 atttctattt acgtgagcat gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt
    18061 tcattatgat gtcaaattcg tcagtaattg atgcaatgtg ttataataat tcagttaaat
    18121 caaaaatgaa atggagaaaa gacatggacg ggttattacc tttatggaaa gaacgcggaa
    18181 tgacaagtca tgattgcgta ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac
    18241 atgggggtac gcttgaccca gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca
    18301 caaaagttat cgaatatttg acagattctg ggaaaatcta tgtaggcgag ataactctag
    18361 gtttcagtac aacgacggaa gatcgatccg gagaacgggt agaaacaaaa ccagtagata
    18421 tgtctttatc tgaaaaaaaa atagatgaag tgatggcttc atttattggt gagatcaccc
    18481 agataccacc aatgtactca gcggtaaaag tcaacggtaa gcggttatat gaatatgccc
    18541 gcaataatga aacagtggag cgtcctgtta gaaaggctca gatttaccgt tttatccgga
    18601 caagtgaatt gacaagagac gaagtgacag gaaccatttc ttggcgcttc gaagtagaat
    18661 gcgggaaagg aacctatgtc cgcacattag ctgtagatac aggcaagaaa cttggttacc
    18721 ctgcgcacat gtctgatctg acccgaacag caagtgccgg tatgtcccag aaggatgctc
    18781 tgacattaga agaagtagct caagctgtga aaaatgggac aatcgatgat tgtttgctgc
    18841 caatagaaaa aggagtggaa cggttcaaac gagtagatat tactgatgaa gtatggcaga
    18901 aagtaaaaaa cggtatgaga cttgattata ctgtatttgc gctcccagaa atgccgtcag
    18961 aagaaattgc tttgttttat aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag
    19021 aaaaaaacaa attaaaacct agtaaagtat tgagaaatga ggaataacga atgaatatca
    19081 tcaagatcag acatccttac caatcagaac aaattccaga agacgatgtc gttttagtgc
    19141 tgggattttt cgacggcgtg catatcggac accagaaagt cataaagaca ggtatggaaa
    19201 ttgcacaaaa agaaggattg aaattagcat tgatgacttt caatcagcat ccatctattg
    19261 tctttaaaaa gatcgatcct agcagcgtga aatatttgac tagccttaag caaaaagaag
    19321 aaaaaatggc cgctttgggt atagattatt tatatgaagt tgattttact tcttcttttg
    19381 ctcatttagc accgcaggaa tttgttgatc aatacatcgt agggttacat gcaaaatatg
    19441 ctgtttccgg ttttgattat acgtatggac caaaagacat tgcagatgtc gctcattttc
    19501 ctgaatatgc caaaggaaga ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg
    19561 aaaaaatcag ctctacccgt attcgtggat tactagacag cggacaagta aaagaagcag
    19621 caaaactatt aggttgcaca tatgaggtag aaggggtagt tgtccatggc gatgcacgag
    19681 gcagactact aggattccct acagcaaatg tcaaagtaaa gagcacggtt catttgccta
    19741 aagaaggtgt ttatgtgtcg gaaataaaaa ttggggaaac atggtatcca gccatgggtt
    19801 caattggaca taatgataca ttcggtgaag gacgggagct gactgtagag ttgtatattt
    19861 tggactttaa tcaagatatc tatggtgaac atgtcgcggt acgctggaat caatttttaa
    19921 gagatcaagt gaaattttct agtgtagagg aattgattga acaactcaag gaggatgaac
    19981 aagcaacagc tgaatatttt tctcagcaat aaaacattat gcataaaatc tcttgtttat
    20041 gtataactag caattacaat aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca
    20101 aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag cttcttcgga
    20161 aataagccga aatttcttga agctgaccac ttctgtcaca acctctttct ttatagaaat
    20221 aaaaggacac tagcaattca tgaaaacgat tgctagagcg tatcatttgc tttagactat
    20281 atatcttggt gtacactcac tttaaggcgg aaataaaagt tatccgtaat aacaggttta
    20341 ggtatgaaaa aggagtgggt actatgtaca aggaattaga aaacaaagtc gcagtagtaa
    20401 ctggtggctc aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga
    20461 atgtagttgt caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg
    20521 aagaggcagg cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac
    20581 agcgtttact tgatacggct ttagaaaatt ttgggacact ggacgtttgg gttaataatg
    20641 ctgggatgga aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtgt
    20701 taaaagtcaa tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag
    20761 agaataataa aaaaggaaat atcatcaata tgtcttctgt tcatgaacag atcccatggc
    20821 caacgtttgc tcattacgct gcttcaaaag gcggggtaaa attatttacc gaaacggtag
    20881 caatggagta tgcaccgaag ggaatcagaa tcaatagtat aggccctggt gctatccgta
    20941 cacctattaa tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga
    21001 tcccactagg acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt
    21061 cagacgaatc cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat
    21121 atccttcttt ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat
    21181 tgatcccggc agttggctgg gggatccagc cactgatttt gaaaaagatt ggtggcagac
    21241 caacaaatga gatattagga actggtattg gagcggtgct tgtcggctta ctcgttcaat
    21301 tattcgcttc tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg
    21361 cattttgggt tattggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta
    21421 aaacgatgcc aatttcgact gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg
    21481 cttttggaga atggtctgga agtatgggga aaatcattgg agcagcagca atcattcttc
    21541 ttgtatttgg ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca
    21601 caaaaggaat tagtctatta gcatcaacaa gtattggtta ttgggtatac agtgcattgc
    21661 ctaaactagt tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt
    21721 taggggcagt gatttatgtg attttccgta aaccaaccgc attttctgaa gacaagagtt
    21781 ggaaagcaac gatcgttgga gccatcttta gtgtgtcagc attggcttat attttttcag
    21841 caaatgaaaa tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta
    21901 cattaggcgg tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga
    21961 ttatcggatt attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa
    22021 gatggtaaaa tttcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata
    22081 aaaaaatagc gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa
    22141 gatacaaggg aaatatctgt ctggatcaat aatttttcat tgatccattg actaaagttt
    22201 acgacataat tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg
    22261 tattgtaaaa gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt
    22321 tctcggaatt gattgattgc tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt
    22381 ggtgcattta gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat
    22441 aggaactcaa aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt
    22501 agttttcctt catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct
    22561 aattcattta ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg
    22621 acttagttga tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt
    22681 gtaaaaatca agtcagaaaa ttctaacttt tatttctcta aagaaattcg ttctctccta
    22741 aagtgatatt atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga
    22801 tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat
    22861 tttactatgt agaaaattca aagccattca atcagtgatt tttttcagca caagaaagac
    22921 tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg
    22981 ataggattga acaaaacctg tataaggtga tgaatagttt cctcgccagt aaaaggttta
    23041 ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg
    23101 aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt
    23161 tatccgtata tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt
    23221 tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat
    23281 ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg
    23341 ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg atttgattga
    23401 tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac
    23461 cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta
    23521 tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac
    23581 ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt
    23641 tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc
    23701 aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg
    23761 aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca
    23821 tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat
    23881 gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt
    23941 gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc
    24001 cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt
    24061 aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat
    24121 gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc
    24181 gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt
    24241 tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa
    24301 atgatgcaag atgaaaatat ccaaatgatc tttccgactt ggggaggaga attattgatt
    24361 gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca
    24421 gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga
    24481 actaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt
    24541 gtactgaaaa caagagaagg agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa
    24601 gtgtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg
    24661 caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg ttgtattgat
    24721 acgatcaggc atttaatcgg tactccttat ggagatgtcc aatcttttaa aagaaagtat
    24781 aatgatggaa agccgatctt gtggtttcta gaaaactgtg agctatcgtc agttgattta
    24841 agaagatcac ttgttcagat gaaactggca ggctggtttg atggcagctc ggggatcatg
    24901 ttcggtagaa gtgcggcaga ggatttgtta gattataaaa tgaaggatgt atacgaagat
    24961 ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca
    25021 caactgacat tgattaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact
    25081 gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtctatca aagaccaata
    25141 aatctaaata gaaaagaaag tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat
    25201 aacatattag aaagtctaat tatctataac tgtcacatcg attaactgtt tcacgtcatt
    25261 gggatttcct gtaggaatag ctactacaga tgcattatca atgatttctt gagttgtttc
    25321 tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg
    25381 ataactagtg attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta
    25441 tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat cataaggggc
    25501 gataggtgtt cctgcaggat aatgaataac atgtaattcg aaatcacccg atgtatctct
    25561 aaaagtatta cctctaatag ctagccaaac acgtgcgtac tcaatttgta gatcagaata
    25621 tcctgctaac ggatctattg actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga
    25681 agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt
    25741 actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc
    25801 agctgaactg gtttgtctga ttgaactttt gtctgtggtc gcagatggat cactattttc
    25861 tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa
    25921 ctttttcata taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt
    25981 ttgaatccgt aaaaccttgt ctatgaatat aaatagagtg tatcttcttt gataaatgtc
    26041 agaaaaagtg tgacaaataa tcatcagtaa aattacttgt cacactattg ataaggagta
    26101 tttaattgaa gagaatgatt aggagaaagg atctccgtac tttttatagt aatcatagaa
    26161 tccaagtttg tatagacgaa tcgttttttc tcttatttct tctgtttcaa aatggactgc
    26221 ttgaagaatc aagtctgtga attctatagg agctgttcca ttcgcagtaa tcaaattacg
    26281 atctttcaca gcttgttgtt ctataaaatc ttctttattc tgataatttt ccgattctaa
    26341 ccaaggaaat tgcgcatttc cggtgtgttt gtaaccttgc aataatccgc tctgagctaa
    26401 ataatcaact gccccacaaa tagcagctac tggtacgtct gtttttaaca atttatcgat
    26461 tatttgatat aaatgatgat tttttatgtc ccatgaattg ccaccgatca taataaaaag
    26521 gtcaactttt tcaggtattt gttcgattaa gtaatcgatt tttgttttta atcctccaat
    26581 ggaagtgacc tctgattgga tagaagcagt tttgttttgc catttctcat tttggtttag
    26641 tgtgctagta atataagcac cttcccaatc ggcatattcc tctaatagaa acgtaatcgc
    26701 agttttcatt ttcaagctct cctttactat ctataacaag gacaacagtt ttaacctaca
    26761 gacattcatt aaaattgggt agcgacagcg aaaacaacaa ttcttaagtt ttagacagag
    26821 tatacagata gtcaacttaa ttaaatacga actaaataaa aatgacaggt aatcattttt
    26881 gtttaccagc attataacat tttgcataca tggatgacaa aagaaccaat tcccacttgc
    26941 ttgagtacaa tattaaaaga aactggaagg caaaaagtaa ttatagaaac gaaaaataaa
    27001 tcatatcggg cactcagaca tgaaaacaac cctagagata tataactagg taaccagttt
    27061 aatgaaagaa aaaatcatcc aagaagtcga ttcttggatt ttttagtgga tttgttcgcc
    27121 aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt
    27181 aaggaggaat ctgtgaaaat agcttttcat atttttgcag attttgactt aatgccgaag
    27241 gtcagctttg cgattaccgt ttatcgagag gctgtaaaag caccgtgtag ctccgatgac
    27301 aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc ttatttcaat
    27361 gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa
    27421 ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt ttctgcgtac
    27481 attcatatcc cattttgcga gcatatctgc tattactgtg attttaataa agttttttta
    27541 gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgaa aataatgcta
    27601 gaaagtcatc cgacagcgaa tttagaaaca ctttacgtag ggggcgggac accaacttct
    27661 ttatctgcta aacaacttga tcgactgtta tcaggtgcta gagagatcct gccttttcaa
    27721 aatggtaatg agtttactgt agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa
    27781 gtaatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt tgataatcgc
    27841 ttattgaaaa aaattggacg taaacacacc gcagaagatg tgtatcaaac tatgcaattt
    27901 ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt accgggacaa
    27961 acactagaag gatatctgga tactttggaa aaagctttgg ctttggatct gcctcactat
    28021 tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt acgacaagga
    28081 agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac gattgaagca
    28141 atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc cggacatgaa
    28201 tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg agctggtgct
    28261 agcggttatc ttggagatat tcgttaccgc aacaaaggac caatccaaca ctatttacgc
    28321 ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg aaaaaatcaa
    28381 atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa aaaacatttc
    28441 tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag ggaattagag
    28501 caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag agggaccttt
    28561 ttggggaaca atgtgtttga gcgctttttg tttgaaaaat agtttaaaga atatttgttt
    28621 tttatctgaa tttaaataaa aaaagttcaa aaatacaata aaggactatt gaaacgttga
    28681 tataactgga tttacatcaa ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa
    28741 atggagtctt tctcttgaca ggagaagggc ttcttgctat atttataagt ggattagcac
    28801 ttagaatata tgagtgctaa tgagaggtga ggaagatgtt aacaaagcga caaaacgaca
    28861 ttttacgtct gatcatccaa cactatacga gtagtggcgt gcccgttggt tccaaaacat
    28921 tgatgtctga gggagtcaag gccagctcag caacgattcg caatgatatg aaagcattag
    28981 aagatgaagg attgctgttg aagactcact cttcttctgg gcggattcca tctgctttag
    29041 gctatcgtta ttacgtggat catttgttaa aacctgctcg cgtagcagaa gatgatttgc
    29101 tgctgattcg tcagtctcta ggcagagaat ttcatgaaat caatgagatt attaagcaat
    29161 ctgctgagat attatctgag ctgacaagct atacgacttt ttcattggga cctgaaataa
    29221 aagaccgccg actgactggc tttcgtatcg tacccttgaa taatcgacag attattgcta
    29281 tcgtcgtaac agatatgggg aatgtagaaa gccaagtgtt cactcttccc gaatatatgg
    29341 gaagtcagga actagaaaaa atggtacaga ttgtaaacga ccgtctagtc ggagatcctc
    29401 tgatgacggt gtaccataaa cttcgtacgg agattccgat gattcttcat aaatatttcc
    29461 aaacgacaga agggatgtct cagttatttg acaccgttct tggtcatgtt tttgaagata
    29521 aggtttatgt cagtggacaa atgaatctct tggattatga gccccgtcaa gatcttgatc
    29581 agttcaaatc gatgttttct tttatgaaag actcagacgg acttacacaa atgatcactc
    29641 cgatggatag caatattcat atcaagattg gtgctgaatt aggcaatgat ttattgcaga
    29701 atatgagtat gattcaagca agttacgaga tagatggtca tggaagaggt acaatagcct
    29761 tgttagggcc tactagtatg ccttattctc ggatgttcgg tctagtcgat gtttatcgaa
    29821 gagaactagc agctaaactt gctgattact accgttcgct tgacttgtca aactcgtaat
    29881 gtgaataacg aagctatttg cagctgatcg ttctctcttt cttagtaaga gcaatcaaac
    29941 agctgactga ataatgaaag gaagactgta tcgtgaaaga aaatcaagaa gaactagata
    30001 aagaaatgac agatgctcag ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg
    30061 gtatttcaga agctgaagcg gaagaattcg aaacagcgaa attaaaagct gagttagaag
    30121 aaatggaaga taaatacttg cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa
    30181 aaaatgagcg tgagcaatta caaaaatacc gttcacaaga tttagccaaa aaattacttc
    30241 catcaattga caacctggaa cgtgcattag ccacagaagt gtcagatgat caaggggcag
    30301 gtttgaaaaa aggcgtggaa atggtcttgg aaagtttgcg taacgcactg gaagaagaag
    30361 gaattgaaaa aatccctgct aaaggagaag cctttgatcc taatcttcat caagcagtac
    30421 aaactgttcc agcgactgaa gatacaccag ccgatacgat tgtagaagta ctacaagaag
    30481 gctacaaatt acatgatcgc gtgttaagac caaccatggt cattgttgca caataattgt
    30541 gcggatataa aacttacact ttcaattaaa gaaggagacg ttaattatga gtaaaattat
    30601 cggtattgac ttaggtacga ctaactctgc agtagcagta ttggaaggaa acgaagcaaa
    30661 aatcatcgct aacccagaag gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg
    30721 agaaatccaa gtaggggaag ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc
    30781 atcaatcaaa cgccatatcg gtgaagcagg ttacaaagta gatgtagaag gtaaatcata
    30841 tacaccacaa gaaatctcag caatgatcct tcaatatatc aaaggattcg cagaagacta
    30901 tttaggtgaa aaagtcgaaa aagcagttat cactgtgcca gcttacttta atgatgcaca
    30961 acgtcaagca acaaaagatg ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa
    31021 tgaaccaact gcagctgctc ttgcctatgg tttagataaa acagatcgcg acgaaaaaat
    31081 cttagtattc gaccttggtg gcggtacttt tgacgtatct atccttgaac taggagacgg
    31141 tgtctttgat gtattgtcaa cagctggcga caaccatcta ggtggggacg actttgataa
    31201 caaaatcatc gactacatgg tagcagaatt caaaaaagaa aacagcattg acttgtctca
    31261 agacaaaatg gctttacaac gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc
    31321 tggcgtatca agcacacaaa tcagcttgcc atttatcaca gcaggagaat ctggaccatt
    31381 gcatttggaa atgacattga cacgtgcaaa atttgatgaa ttaacagctg acttagtaga
    31441 acgtacaaaa gtaccagtac gtcaagcgct aaaagatgca ggtttatcac aatcagaaat
    31501 cgacgaagtt attttagtcg gtggatctac tcgtatccct gcagttgtag aagcagtacg
    31561 taaagaaaca ggaaaagaac caaacaaatc agttaaccct gatgaagtag ttgctatggg
    31621 tgctgctatc caaggtggcg tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt
    31681 tacaccattg tcattaggta tcgaaacaat gggtggagtg ttcactaaat tgattgaccg
    31741 caatacaacg attccaacaa gtaaatcaca agtattctca actgcggcag ataatcaacc
    31801 agcagtagac atccatgtat tacaaggtga acgcccaatg gctgctgaca acaaaactct
    31861 aggaagattc caattgacag atattccagc agctcctcgt ggtattccac aaatcgaagt
    31921 aacattcgat attgataaaa acgggattgt aaatgtatct gcgaaagatc taggtacaca
    31981 aaaagaacaa aaaatcacga tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg
    32041 tatggtaaaa gatgcagaag caaatgcaga agcagataaa gctcgtaaag aggaagttga
    32101 cctacgtaat gatgtagatg cattgctatt ctctgtggac aaaacattga aagaattaga
    32161 aggcaaagtg gacgaagaag aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc
    32221 cgctgttgaa gcaaacaaca tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat
    32281 cgttcaaaac ttgactgtta aactatacga acaagcggca caacaacaag ctcaagaaaa
    32341 cccagaagca gctcaaggcg gagcagatga tgtcgttgat gctgattttg aagaagtcga
    32401 tggcgacgat aaataatcag ttcactgaat ttcgagagaa ggccaaagtt gctactttgg
    32461 ctttttcttt tgaaaaatag tttgaagtat cggtttttgt ttcaaacaaa atatgatatg
    32521 ataacaagcg acgtgaaaat gatgagaacc aataggagga aagcctatgg caacaaaacg
    32581 tgattattat gaagtcttag ggctctcaaa aggagcatca gaagatgaaa taaagaaagc
    32641 ttatcgtaag ctttcgaaaa aataccatcc tgacatcaat aaagaacctg atgcggaaga
    32701 aaaattcaaa gaagtatcag aagcatatga gattttaagt gacccgcaaa aacgcgctgc
    32761 atatgaccaa tacgggcatg caggcacaga tccaaattac ggtgctggtg gagctggtgg
    32821 cggatttggt ggattcggcg gcttctctgg tggcggcttc ggtggttttg aagatatctt
    32881 cgactctttc tttggaggag gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc
    32941 tgatttgcaa tatacggtcg acttgtcttt tgaagaagcg atttttggtg tagaaaaaga
    33001 aatcaagtat aatcgggaag aaatctgtca tacttgtggt gggaacggtg caaaaccagg
    33061 cacacaacca actacttgtc ataaatgtca tggtgcaggt acgattaatg tagaacgcca
    33121 aacaccactt ggacgagtga tgagccgtca aacttgtgat gtttgtcatg ggacagggaa
    33181 agaaatcaaa gaaccttgtc caacctgtca tggctcagga catgagaaga aagcacattc
    33241 agttaaagtg aatgtaccag ctggtgtaga agaaggacaa caaatgcgtt tagctggtca
    33301 aggagaagct ggagaaaacg gcggaccatt tggtgatttg tatgtggtct tccgtgtaga
    33361 agaaagtgat atctttgatc gtgaaggttc tgaaatttac tatgaattgc ctttaagttt
    33421 tgtacaagca gctttggggg atgaagtcca agtaccaact gtccatggag atgtgaaatt
    33481 gaagatccct gctggaacac agacaggtac gaacttccgt ttacgtggaa aaggcgctcc
    33541 gaagttacgt ggtggtgcaa caggcgacca acatgtcaaa gtaaaactga tcacaccgaa
    33601 aaatctaaat gaacaacaaa gagaagcatt acgtgcattc gctgaagctg gcggcatcaa
    33661 agttgaagaa cagcaagaag acggtttctt cgataagttt aaagatgctt ttggcgggaa
    33721 aaagcggaag tagtttttct agtaaaaacg ctaagtcatt aataattaaa aaccagacga
    33781 acaatcatat gttcgcctgg tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca
    33841 gctttagaat atgataatcg tcatagtttc taggggtata accagtgaga gaatggaact
    33901 tgttcaatcg atattgcaag gtgtttttat gaataaataa ttcttctgag caatttttga
    33961 tgcttccgtt atttttactg aagcatgcca aagtcttctt gaacaaatgt acttcttctt
    34021 ctgtcaggct tttcaaaata cgttttacat ataatgttcg ttgatctgac gggattgctg
    34081 gtaaaacgat cccgaggtct aaatcttcaa aaaagcggat ctcgttcaca gattgatgga
    34141 aagctatcca ttcgcaaact tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg
    34201 tataacaaag cgacccaatt ccaaagttta gctgtaactg atacatttta ttaaactttg
    34261 tctggatttt atcaagcaat gatagcaatg aggctctgga aatccgattg actaggataa
    34321 tgatttgttg tctatgtaca gtgagcaaag cctgtccgta ataagagaag accctttcga
    34381 gcaactccaa taaaggattg atccgttctt gacacaattg ctgattgtca gaaagttgtc
    34441 ccacaactac ataatatgga gaagaaaaat caagctcaat taagtgaggg tggtatcgac
    34501 tatgtacttg atatcatcaa tgaaccgatg gtactttctt atgcgatgga acaggtagag
    34561 cgattgcttg tttgtgcagg aaaacaagcg atgcggctga tacgattagg tgagaaaata
    34621 agaagataaa aatacacggt cccttcatgg cgaccgtgca tttttggttt ttattaaata
    34681 tttaatactt tattcaagaa atcttttgtc cgttcatttt gcggattttc aaagatttgt
    34741 tgaggcgttc catcttctaa aaagtttccg tcgtcaatga acatgacgcg attggccact
    34801 tctttagcga accccatttc gtgcgtaacg ataaccatcg tcataccttg ttctgctaac
    34861 tttttcatga cgttcaagac atcaccgacc atttcaggat cgagtgcaga agtaggctca
    34921 tcaaaaagca tgatgtcagg gttcatcgct agagcacgag caatagccac acgctgtttt
    34981 tgtccaccag aaagcatttc aggatacata tcttttttat cttctagacc aactgtctgc
    35041 agtaatttga ttgctttttt ctctgcttct tctctagatt ctttcttcag gtccatcggt
    35101 gctaaaatga tgttttccaa aatagaaaga tgagggaata ggttaaagtg ttggaatacc
    35161 attccgatat gttgtctcac caagttgata tctgtgcttt tatccatcaa gtgggcgccg
    35221 tcaataataa tttcaccgtc agaaggttct tctaaacgat tcaagcaacg caagaatgta
    35281 ctttttcctg aacctgaagg gccaatgata cagacgacat ctccttcgtt gatggataca
    35341 ttgatgtcgt ttaatactgt gttatcgccg tattttttga ctaaatgctc aactaagatt
    35401 ttttctgcca tcttacttca ccttcttttc taaaactttt gctaattttg tcaatgctgt
    35461 gatcaaaatc aagtacatga tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg
    35521 tgcaacaatg atcttaccag tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt
    35581 tgtgtctttt aatgagataa cgaactgatt gacgaatgat gggatcatga tgcgtatcgc
    35641 ttgcggcaaa atgatttttt gcattgtccg attataagaa agacctaaac ttctggaagc
    35701 ttccatttgt ccgacaggaa cggcgttgat cccgcctcga acaatctctg cgatataagc
    35761 acttgcgtta agagtcaagg tgataacacc agcaaggaag tcaggaatcg taaatccaac
    35821 gataccaggc aacccaaaga aaataaagaa cgcaagaacc atcattggaa ttcctcggat
    35881 gatatcgaca taaatactag caattgtgcg aagagcgcgt attggtgcta cactgaataa
    35941 tccaaaaatc acaccgatta ctatagccaa agcaaaggag atcaaagtta ggagaattgt
    36001 tctccaaagt cctttcaaca atgcttgata gttgttttgg ataagtccaa gaaatgttga
    36061 ttcatcagca gtggaagact cactggaagt ttctgaacca tccgctacat attctttaat
    36121 aatcttatcg tattcgcctg ttctttttaa ttctttcaat ccttcattga acatattaag
    36181 aagctcagga ttttgtcctt ttttaacggc aaatgcatat tttccgccag attctttttt
    36241 gatcggtgtt tccaattctt gtccttgttt gatcgcataa ccgattaccg gataatcatc
    36301 catcattgca tcgattgcgc caacttttaa cccgtcgtac aattgatcag cagcatcaaa
    36361 atatttgata gaatagccat attcagattc atggtcgctt aaaaattcag cactttcggt
    36421 accaactttt gctccgactg tttttccttt caaatcttca taagacttga tcgaagtatc
    36481 tccttttttg acagctaatt ggatcccgct atcaaagtaa gaatcagaaa aatcatagct
    36541 tttctttcgt tcatctgtta cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc
    36601 caaagcttgc aaagcagcag agaaaccacg gtagtccatt cggatgttaa atccttgcag
    36661 tttggcgatg ttttccatca attttacatc gatgccttcg tactcgccct ttgcgttttg
    36721 aaattcaaat ggagcaaaag cagagtcact ggcaattacg tatacgtctt ttttgggctc
    36781 tattttttgc attgtctcgc ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc
    36841 aacatgacca aacgcaaatg tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa
    36901 atgctttctt ttcataatca acctcctaaa tttccaatat tttattatta taggcgaaaa
    36961 gtgctgaatc agacaagact gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat
    37021 tttatgttat aaaacataac atgtataata agataaaaaa gtggcgaaag tatgttcggt
    37081 gtggtataat tttttagtaa ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa
    37141 atgaaaagga atgaatgaaa aggaggacga agaatgattg agagagatac ctcccgacac
    37201 tttgagcttg tctcgaaata ccaaccggct ggtgatcagc ctgaagcgat taaccaatta
    37261 gtggatggcg tagtcggcgg gaaaaaagca caaatcttat taggtgcgac tggaacaggg
    37321 aaaacatata cgatttccaa tcttattgag aaagtgaata aaccaacact gattattgcc
    37381 cataacaaaa cattggctgg tcagctgtac ggggaattca aggaattttt ccctaacaat
    37441 gctgttgaat attttgtttc ttattatgat tattatcagc ctgaagctta tgtgccttca
    37501 agcgatacgt atatcgaaaa agattcaagt gtaaatgatg agatcgacaa attacgacat
    37561 tcggctacta gttcattatt agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt
    37621 atttttggat taggctcgcc ttttgagtac caaaaacaag tagtttcgat tcgacaggga
    37681 gcagaactag atcgcaatca gttgattcgt gacttagtca gtatccaatt cgaaagaaat
    37741 gatatagatt ttcagcgcgg tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg
    37801 gcttcaagag atgaacgtgc cttacgtgtg gagttttttg gagatgaggt cgaacgtatc
    37861 cgagaagtca atgcactgac aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc
    37921 ccggcgactc actttgtgac gaatgatgaa catatggagc atgccgtagc gaatatcaaa
    37981 gcggaattag aacagcgttt aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa
    38041 cgtttagaac agcgaaccaa ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg
    38101 ggaattgaaa attattctcg gcatatggac ggacgtaaag aaggtgaacc gccttatacg
    38161 ctattggatt tctttcctga agattttttg atcgtggcgg acgaatcgca cgtaaccatg
    38221 cctcaaatca gaggaatgta taatggtgac cgagcgagaa aacagatgct agtagattac
    38281 ggtttccgtt tgccatctgc tttagacaat cggccacttc ggttagaaga gtttgaaaaa
    38341 catgtcaatc agatcattta tgtctctgcg acgccaggac catatgaaca tgaacaaaca
    38401 gataccgtta tccaacaaat catccgtccg acagggctgc ttgacccagt gatcgaagtt
    38461 cgtccaatca tgggacaaat cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa
    38521 gaccagcggg tgtttgtaac gacattgacg aaaaaaatgg cagaagacct cacagattat
    38581 ttcaaagaat tagggatcaa agtcaaatat ttgcatagtg atatcaaaac gctggaacga
    38641 acagaaatca ttcgagatct tcgattaggt gaatttgatg tgttagttgg aatcaatttg
    38701 cttcgtgaag gattagacgt acctgaagtt tcccttgttg ctatcttgga tgcggataaa
    38761 gaaggctttt tacgtagtga acgttcctta gtccaaacga ttggacgagc tgctcggaat
    38821 gaagaaggta aagtaatcat gtacgcagac aaagtaacag attctatgcg tctagcgatg
    38881 gatgaaacgt caagacgtcg tacgattcag cagaagtaca atgaagaaca tgggatcgtt
    38941 ccaaaaacga tcattaaaga aatccgagat ttgatttcta ttacgaaaga atcggaagat
    39001 gatacaaaag aagcggtaca agtctcgtac gaagaaatga cgaaggaaga aaaagacacg
    39061 ttattgatga aattagaaaa agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca
    39121 gccgcgaatg tgcgagatat gattttagaa ttaaaagctt caaagtagga ggacaacatg
    39181 gctaacgata aaattacgat tcatggtgca cgtgcccata acttgaaaaa tatagatgta
    39241 acgattccac gagataaatt cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc
    39301 ctagcatttg atacgctgta cgctgaaggg cagcggagat acgtagagag cctttctgct
    39361 tatgctcgtc agtttttagg acaaatggat aagccggacg tagatagcat cgacggattg
    39421 agtccggcga tttctattga tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt
    39481 actgtgacgg aaatcaatga ctatttacgc ttgttgtttg cacgtgtagg acatccgatt
    39541 tgtcccaacg atcatatcga gattaccagt caatcagtag atcaaatggc agatcaagta
    39601 atggctttgc ctgagcgcac aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa
    39661 ggacaacata aaaaaatatt cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc
    39721 gatggtgaga tctatgattt aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat
    39781 atcgcgatcg tcatcgaccg gatcattgtg aaagaaggga ttcgttctcg cctatttgat
    39841 tctttagagg cggccctgcg attagcagac ggctatgctc tggttgacgt gatcggtgaa
    39901 gaagaaatgt tgtttagcga acactatgcc tgtccttatt gcggatttac tgttggcgaa
    39961 ctagaaccaa gactgttctc attcaatgcg ccttttggag cttgtccaga ctgtgatggt
    40021 ttaggtgtca aattagaagt cgatcaagat ttagtgattc cagatagaac gaagacactt
    40081 agtcaaggtg ctatcgttcc atggaatcca atcagctctc agtattatcc tcaaatgtta
    40141 gcgcaggctt gtgaaagttt cggtatcgat ttagatacac cattcgaaga attaccggaa
    40201 gagcatcagg agatcatctt gaatgggaca gatgatcttt tccatttcca ttatgaaaat
    40261 gattttggtg gcgttcgcga tgtagaaacg actttcgaag gggtattgaa gaatatcgag
    40321 cgtcgttatc atgatacgaa cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca
    40381 gaattaactt gtcagacatg taaaggttat cgattgaatc cccaagctct atctgttcaa
    40441 atcaatggaa cgaatatcgg gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc
    40501 ttctcaaact taacgttatc tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa
    40561 gtaaatgaca gactttcatt cttggaaaat gtgggactag actatctaac tttgagtcgc
    40621 gcttccggaa cattatctgg gggagaagcg cagcgaatca gattggctac acaaattgga
    40681 tcgaatcttt ctggagtttt gtatatttta gatgaaccat cgatcggatt gcatcaacgc
    40741 gacaacgatc gactgattgg ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt
    40801 gtagtcgaac atgatgaaga tacgatgtgg gcagcagatt atttgattga cgtcggacct
    40861 ggggccggag aacaaggcgg acaaatcgtg gctgcaggca ctcctgaaga agtagctaat
    40921 gatgaaaatt ctttgacagg gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa
    40981 gaacgccgta aaggtaatgg gaaagcaatc aagatcactg gtgcttcaga aaacaatctg
    41041 aaaaatatta gtgttgaatt tccattagga gaatttgtag cagtcacagg tgtgtctggt
    41101 tctggaaaga gtacattggt caatagtatc ttaaagaaat cgttagcgca aaaattaaat
    41161 aagaattctg ctaagccagg taaattcaag acaatttccg gctacgaaag tatcgaaaag
    41221 atcatcgata tcgatcaaag cccaatcggc cggacgccga gaagtaatcc agcgacttat
    41281 acaagtgtat ttgatgatat ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg
    41341 ggttataaga aagggcgttt tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc
    41401 ggggatggaa ttattaagat cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa
    41461 gtatgtcatg gtaaacgata taattctgaa acattagaag tgcattacaa aggaaaaagc
    41521 attgctgata ttttggaaat gacagtagag gatgctgtag aattcttcaa gcacattcca
    41581 aagattcatc gcaaactgca aacgattgtt gatgttggct taggttatgt gacgatgggg
    41641 caaccagcaa cgacattgtc cggtggtgag gcacaacgga tgaaacttgc cagtgaattg
    41701 cacaaaatct ctaatggaaa gaatttctat atactagatg aaccaacgac aggacttcat
    41761 agcgatgaca tcgcccgctt gttgcatgta ttacaaagat tagtagatgc tggtaacaca
    41821 gttttagtga ttgaacacaa tctagatgta atcaaaacag cagattatat cattgattta
    41881 ggaccagaag gtggagaagg tggaggaacg atccttgcga ctggaacacc agaagaaatc
    41941 attaacgtaa aagaaagtta tacaggtcac tatttgaaaa aaataatggt ataaagagga
    42001 tagatagctg agatagttaa aaaaacataa aaaaatgaat ttttgcttgt tttattaata
    42061 tcagtgttct agaatcatta catggtagtt ctttctacac tgattggtat agataaaaac
    42121 ttcctataag ttcaatccgt tcaaaaaaga attgagcaat cggaggactt tcctttgatt
    42181 gcttttttct ttatggtatg atattccttg tgtagttgga aaagaaaaac aagagattat
    42241 gagtaagtga gagataatga tgaacagtat tgaaaatgaa gaatttatcc ctatattatt
    42301 aggaagcgac atgaacgttt atggtatggc acgttctttt aatgaagcat acggcaaaat
    42361 ctgccaagca tatgcttcgg atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt
    42421 agaagttatt ccaggatttg ataaagaccc tgtatttatc gagacgatgc ttcgtttggc
    42481 aaaagaaaga tattctgata agagtaaaaa atatcttttg attgcttgtg gtgatggata
    42541 tgcagaattg atctcccaac ataaacaaga gctgtcagaa tattttatct gtccatatat
    42601 tgattattct ttatttgaaa ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga
    42661 atatgatctt ccgtatccta aaacattgat cgtcagagaa gaaatgttag taaatggaca
    42721 tttggaacag gaattgcctt ttgaattccc agttgctttg aaacctgcaa acagtgtgga
    42781 gtatctttct gtccaatttg aaggaagaaa aaaagctttt attttagaga ctagagaaga
    42841 gttcgattta attttaggcc ggatatacga agccggttat aaaagtgaaa tgattgtaca
    42901 agattttatc ccgggtgatg atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga
    42961 tcatcaagtt cggatgatgt gccttggtca tccactatta gaagatccta caccagcatc
    43021 gattggtaat tacgttgtga tcatgccaga ttataacgaa aaaatctatc aaaccattaa
    43081 ggcatttttg gaaaaaatcg aatatacagg ctttgccaat ttcgatatga agtatgatcc
    43141 tagagatgga gaatataaat tatttgaaat caatttgaga caaggtcgta gcagtttctt
    43201 tgtgacattg aacggcttga atctggctcg ttttgtcaca gaagatcgag tgttcaacaa
    43261 accctttgtt gaaacgacct atggaacgaa tcaatcagat aaagcaagat tatggatggg
    43321 tgtgccccaa aaaatctttt tagaatatgc tcgggaaaat gaagacaaaa aactagctga
    43381 gcaaatgatc aaagaaaatc gttacggtac tactgtattt tatgaaaaag accgatcaat
    43441 caagcgctgg ttgttgatga aatatatgtt ccataactat attccaagat tcaaaaagta
    43501 ttttcatgtg aaagaaggct agttcatgga gaattttttc agtattttag gcggaatggg
    43561 cacgatggcg acagaaagtt ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga
    43621 tcaagaatat ttgaattatg tcttattcaa tcatgcaaca gttcctgatc gtacagctta
    43681 tattttagat cgatcagaag aaaatccaat gccgtttctg ctggatgata ttgagaaaca
    43741 aaatttattg cgaccaaact tcattgtgtt gacttgtaac accgcacact acttttttga
    43801 agagttacaa gcggcaacgg atattcctat cttgcatatg ccaagagaag cagcaaatga
    43861 acttgtccgt cagcatacga cagggagagt agcgattcta ggaacagaag gaagtatgaa
    43921 agctggaatc tatgaacgcg aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac
    43981 tgcgcttcaa gaaaaaatca attacctgat ctatcatgag atcaaggaat cggattattt
    44041 gaatcaagag ctctattacg aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa
    44101 agtcatccta ggttgcacag aattatcttt gatgcatgaa tttgctgaag ataatcatta
    44161 cccagtgatc gatgctcaat ctattttagc ggatcgaacg atcgaacgtg cattagctga
    44221 aagaagcgag gcattggata cagcatcgga aaagtaaaaa aattggattt ggggcaaata
    44281 tcctaaaaga tatttggtcc aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac
    44341 gcccgggatg atataatttt attatgcaca atgtgagagg ggttcgtaaa tatgacagat
    44401 aatttgcaat tagtcatcat aacaggtatg agtggtgcag ggaaaacagt agctatccaa
    44461 agttttgaag atatgggcta tttttgtatc gataatatgc cgcctcgatt gattccaaaa
    44521 ttctgggaat tgatcaaaga atcaggtaaa gtcacaaaaa tggctttagg agtggatttg
    44581 cgttctcgtt ccttctttga agaaatccaa aacatgctga tcgaaatcga aaatacgaac
    44641 ttcatcgaca cttctatctt atttttagat gcttcagacg aagaactagt ggctcgctac
    44701 aaagaaacaa gacgagcaca tccaatggcg atggacggtt tgatcactga agggatacgt
    44761 aaagaacggg cgatactaga cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca
    44821 acattgacac cacgacaatt acgtgaaaaa atcaatgagt cattcaaatc aaagggagac
    44881 caaggatttc ggatcgaatt tgtatcattt ggttttaaat acgggttgcc gatcgattcg
    44941 gatattgtga tggatgtgcg ttttttgccc aaccctcatt atatcccgga actaagacca
    45001 ttgaccggaa aagatcagcc agtctatgat tatgtgatgt cctttgatga aacgcaatca
    45061 ttctaccata aatttttgga tcttctggaa actgtattgc ctggatacat taaagaagga
    45121 aaaagcagct tgacagttgc aatcggctgt acaggcggtc aacaccgttc tgtggcattg
    45181 actgaacgtt tggcaaatgc tttagggaaa aactataaag tcaatgtgac acatcgggac
    45241 aaggataaaa ggaaagagac ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa
    45301 aaccaaagat cgtagtggtc ggcggtggca ctggtctgcc agttatcctg aaaagtttac
    45361 gaaatcaagg agcagatatc acagcagtag tgactgttgc agatgatggt ggaagcagcg
    45421 gtgcaatcag agaatcgatc gctatggctc ctcctggtga cttgagaaat gtcttagtgg
    45481 cgttatcgga tatgccacaa ttctatgaag atattttcca ataccgtttc aaaaaggaag
    45541 atcagttttt agccaatcat acgatcggta atctgatcat tgccgcagtt tccgagatgc
    45601 ggggaagcac ttatgaggcg attcaattat tagcaaaaat gatgcatgta gatggacatg
    45661 tttatccttc ttcagaaact cctttgacct tgcatgctgt ttttaaagac ggaacgactg
    45721 cagtaggaga atctaaaatc gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca
    45781 acacaaatga taaaagtgaa gccaaagcgg ctcgaaaagt cgtttcagca attatggacg
    45841 ccgacatggt cgttttggga cccggaagtt tattcaccag tatccttcca aatttagtga
    45901 taccagaaat cggcgaagca atgctcaaga caactgctga aaccgtctat atctgtaata
    45961 tcatgaccca aaaaggggaa acggaacatt tcacagatgc cgatcatata cgtgttttac
    46021 acaagcattt gaatcagcct tttatcgata cagtattggt caatacggaa aaagtgcctg
    46081 aagattatat ggacccagag atctatgatg agtatctagt acaagtcgca cacgacttca
    46141 acggattgag aaatgaagga tgtcgagtga tttccaccga ctttttagaa ttgaaagatg
    46201 gtggtgtctt ccacgatgga gacaaagtag tagaagaatt gttccgttta gttttcggtt
    46261 caaaatatta agcaatttat tgttggagaa aggaggagaa taggatgtca tttgctgcag
    46321 atgtgaagaa agaactaact ggtttggaag ttcatcgaga acatgcgaaa gcggaattag
    46381 ctgctttgct gcgaatgaac ggctctttag gtattgtgaa ccagcagttt attttgaacg
    46441 tccaaacaga aaatgctgca attgccagac gtatctattc tcttttgaaa gatcattatc
    46501 aggtacgaag tgagctactc gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata
    46561 tcgtccggtt gaagcaaggc acaaaagatg tgttgatgga tcttgatatc atggatggta
    46621 tgatgttcaa tggacacgtt tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt
    46681 acttacgtgg tgcttttatg gcctcaggtt cagtcaataa tccggaaacg agtcgttatc
    46741 atttggaaat attttctatt tatgaacagc ataaccaaga catttgcgat atgttaaatt
    46801 attacggtct acatgctcga acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag
    46861 gagcagaaaa aattgctgat ttcttaacat tgattggtgc aaccaactca atgctccgat
    46921 ttgaagatgt tcgaatcgta cgggatatga gaaattcggt caatcgtcta gtcaattgtg
    46981 agacagcaaa tctgaacaag acgattgatg cggcttctaa acaaatcgaa aacatcgaat
    47041 tgatcgaagc aaaggtcggt ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac
    47101 ttcggcttca acatccagag gtcagcttga aagaattagg ggaaatgatt ccctcaggtg
    47161 caatatcaaa atcgggaatt aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac
    47221 tgcgtgaaga agcagtttaa tccaaagaaa aaactgatac atgaagcagg agatgcgaat
    47281 gtatcagttt tttttaaaag ctatacttgc ttttcttccg tcaaactata attccaactt
    47341 tactggagta acataatgtt gaaaggcttc tattgttttt taatacaatc gtataaaaag
    47401 cggggagaca actgaatgaa gattttagtt ttatacataa acttttgctt aatatagaag
    47461 tcctgatata gataggatca agtatttatc tataactgga gattaattaa aaactggaag
    47521 gaactgctcg atcaaccttg agaactgttt ttcttacttc tggtcaagat tctcgtggtt
    47581 ggtggataag gatggttgat aagttcattg gattagctag tcgaaaacaa ccaggtgtaa
    47641 ttaatgtaga agttttaaca aggggatgga gtgatttatt cagtgataat cataagttca
    47701 taaatagtaa ttgggatgta tggttggtat acgttatacc tgaacttgaa ctatcaagat
    47761 ttggtttaga atttgagaat cgatgtttat aaagggaggg caaaaaatgt gcttttttac
    47821 aacttttact atcattatga ttgcagtact ttttattttt ctagatatag caaagcgaaa
    47881 tacagcattt ttattatatc gggttttact tcgagcaggt ctcataacat ttatatcgat
    47941 cgttggattt tttctattca cagttattgt atttatttgg agaactacag caccaccatt
    48001 gccagagatt acatatggag aatttccgtt tagtcttgaa tatgaaatag atgaggaaag
    48061 aattttgatt gaagatacgg tgattgcaga atttatagga agttttagag ggaatgtgac
    48121 aactcaacct agacgaagtt ggagtataaa acttttaaat aatagtggag agttgaaagc
    48181 atttcattat gaattcttaa taatagaaag tgaagatgta acaatcactt taagtagtgg
    48241 ctctgctgga tattttatga atgatggtgg aagcaagcca cagctcacag tccgtgatcg
    48301 tattgcaggt tttatgcatt tttctcacta tgacttagaa gaagctcgta aaatacttgc
    48361 agaatatggg atcacattaa tcaattggga atatgcgcca ccgataagaa attttttcaa
    48421 tgattattat tagtcttata aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt
    48481 ttccattttt tgtttgatag atatatagca ttgttgaagc atgggatagt ggtgccaatt
    48541 ttatcaatga ggttggtcag gcagttaaca taattggtga tgctataaat ccatttaatt
    48601 ggaattggag gaattaggtg tggaaacagt gagattatgg atattcatga aaaattttcg
    48661 ttcggctttg agatatgtta ttctggaatc aaaatcaggt tattatctga ttgacacaga
    48721 tcactatact ttttggggag gctacctttt tccgattttt aattggataa tcccccataa
    48781 ggttatgaaa attaacttgc ctgcagaaga aataaataga ttaatactca acaaagattt
    48841 acaaaggaaa agagagaaag aacattctaa tgttgcttgg ataggtccag catttgcagt
    48901 agtgacttct tcaattgtcg gtcctatgct agagagtatt tatagtgatt tttctattgg
    48961 aatacgttat ttatttgtta ctttaagtat agctgttctt ttgtgcataa agggttatgt
    49021 tagcaagaag aataaagata ttttaacact aatcggtgta aacaatttat ctagtactaa
    49081 aattatactg tttcctgaat ctataaaaga aggatcaagg ttgttgataa tttttataat
    49141 gcttatactg ttttctgttt tatttggggt gatgtttata
//
LOCUS       DANJJH010000007        48503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000007 DANJJH010000000
VERSION     DANJJH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="PWS53_000421"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="PWS53_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL0870694.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="PWS53_000422"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="PWS53_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL0870695.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="PWS53_000423"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="PWS53_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL0870696.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="PWS53_000424"
     CDS             4735..5628
                     /locus_tag="PWS53_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870697.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="PWS53_000425"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="PWS53_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0870698.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="PWS53_000426"
     CDS             7565..8422
                     /locus_tag="PWS53_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL0870699.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="PWS53_000427"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="PWS53_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL0870700.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="PWS53_000428"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="PWS53_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL0870701.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="PWS53_000429"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="PWS53_000429"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0870702.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="PWS53_000430"
     CDS             11178..11936
                     /locus_tag="PWS53_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0870703.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="PWS53_000431"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="PWS53_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL0870704.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="PWS53_000432"
     CDS             12864..13895
                     /locus_tag="PWS53_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0870705.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="PWS53_000433"
     CDS             13892..14659
                     /locus_tag="PWS53_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0870706.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="PWS53_000434"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="PWS53_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL0870707.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="PWS53_000435"
     CDS             16624..16944
                     /locus_tag="PWS53_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL0870708.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="PWS53_000436"
     CDS             16944..17294
                     /locus_tag="PWS53_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL0870709.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="PWS53_000437"
     CDS             17461..18399
                     /locus_tag="PWS53_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL0870710.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="PWS53_000438"
     CDS             18414..19244
                     /locus_tag="PWS53_000438"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0870711.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="PWS53_000439"
     CDS             19288..20313
                     /locus_tag="PWS53_000439"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0870712.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="PWS53_000440"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="PWS53_000440"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0870713.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="PWS53_000441"
     CDS             21391..21831
                     /locus_tag="PWS53_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL0870714.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="PWS53_000442"
     CDS             21834..22436
                     /locus_tag="PWS53_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0870715.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="PWS53_000443"
     CDS             complement(22495..23601)
                     /locus_tag="PWS53_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0870716.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="PWS53_000444"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="PWS53_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL0870717.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="PWS53_000445"
     CDS             complement(25149..27494)
                     /locus_tag="PWS53_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0870718.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="PWS53_000446"
     CDS             27773..28675
                     /locus_tag="PWS53_000446"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL0870719.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="PWS53_000447"
     CDS             28888..30663
                     /locus_tag="PWS53_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL0870720.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="PWS53_000448"
     CDS             complement(30729..32315)
                     /locus_tag="PWS53_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL0870721.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="PWS53_000449"
     CDS             32566..32790
                     /locus_tag="PWS53_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL0870722.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="PWS53_000450"
     CDS             32932..34683
                     /locus_tag="PWS53_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0870723.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="PWS53_000451"
     CDS             34683..36467
                     /locus_tag="PWS53_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870724.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="PWS53_000452"
     CDS             36793..37644
                     /locus_tag="PWS53_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0870725.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="PWS53_000453"
     CDS             37698..39449
                     /locus_tag="PWS53_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0870726.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="PWS53_000454"
     CDS             39453..39623
                     /locus_tag="PWS53_000454"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0870727.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="PWS53_000455"
     CDS             complement(39663..40565)
                     /locus_tag="PWS53_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0870728.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="PWS53_000456"
     CDS             40796..42199
                     /locus_tag="PWS53_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0870729.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="PWS53_000457"
     CDS             42199..43632
                     /locus_tag="PWS53_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0870730.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="PWS53_000458"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="PWS53_000458"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL0870731.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="PWS53_000459"
     CDS             44917..46644
                     /locus_tag="PWS53_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL0870732.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="PWS53_000460"
     CDS             46659..47009
                     /locus_tag="PWS53_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0870733.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="PWS53_000461"
     CDS             complement(47101..47961)
                     /locus_tag="PWS53_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL0870734.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="PWS53_000462"
     CDS             48053..48481
                     /locus_tag="PWS53_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0870735.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
BASE COUNT        15673 a         8521 c        10408 g        13901 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca ttt
//
LOCUS       DANJJH010000008        43874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000008 DANJJH010000000
VERSION     DANJJH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(144..1247)
                     /locus_tag="PWS53_000463"
     CDS             complement(144..1247)
                     /locus_tag="PWS53_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0870736.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1876..2346)
                     /locus_tag="PWS53_000464"
     CDS             complement(1876..2346)
                     /locus_tag="PWS53_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL0870737.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2676..4316
                     /locus_tag="PWS53_000465"
     CDS             2676..4316
                     /locus_tag="PWS53_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL0870738.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4470..5792
                     /locus_tag="PWS53_000466"
     CDS             4470..5792
                     /locus_tag="PWS53_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL0870739.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5860..6294)
                     /locus_tag="PWS53_000467"
     CDS             complement(5860..6294)
                     /locus_tag="PWS53_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870740.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6359..7196)
                     /locus_tag="PWS53_000468"
                     /pseudo
     CDS             complement(6359..7196)
                     /locus_tag="PWS53_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7938..8564)
                     /locus_tag="PWS53_000469"
     CDS             complement(7938..8564)
                     /locus_tag="PWS53_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870741.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8859..9194)
                     /locus_tag="PWS53_000470"
     CDS             complement(8859..9194)
                     /locus_tag="PWS53_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0870742.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9181..9465)
                     /locus_tag="PWS53_000471"
     CDS             complement(9181..9465)
                     /locus_tag="PWS53_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0870743.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9521..11044)
                     /locus_tag="PWS53_000472"
     CDS             complement(9521..11044)
                     /locus_tag="PWS53_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0870744.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(11037..12212)
                     /locus_tag="PWS53_000473"
     CDS             complement(11037..12212)
                     /locus_tag="PWS53_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0870745.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12216..12401)
                     /locus_tag="PWS53_000474"
     CDS             complement(12216..12401)
                     /locus_tag="PWS53_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870746.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12367..14061)
                     /locus_tag="PWS53_000475"
     CDS             complement(12367..14061)
                     /locus_tag="PWS53_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0870747.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(14058..14531)
                     /locus_tag="PWS53_000476"
     CDS             complement(14058..14531)
                     /locus_tag="PWS53_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL0870748.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14600..14755)
                     /locus_tag="PWS53_000477"
     CDS             complement(14600..14755)
                     /locus_tag="PWS53_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL0870749.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14889..15269)
                     /locus_tag="PWS53_000478"
     CDS             complement(14889..15269)
                     /locus_tag="PWS53_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0870750.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15273..15488)
                     /locus_tag="PWS53_000479"
     CDS             complement(15273..15488)
                     /locus_tag="PWS53_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870751.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15491..15898)
                     /locus_tag="PWS53_000480"
     CDS             complement(15491..15898)
                     /locus_tag="PWS53_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL0870752.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16164..17639)
                     /locus_tag="PWS53_000481"
     CDS             complement(16164..17639)
                     /locus_tag="PWS53_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0870753.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17629..18477)
                     /locus_tag="PWS53_000482"
     CDS             complement(17629..18477)
                     /locus_tag="PWS53_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL0870754.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18514..18876)
                     /locus_tag="PWS53_000483"
     CDS             complement(18514..18876)
                     /locus_tag="PWS53_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870755.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(18877..19035)
                     /locus_tag="PWS53_000484"
     CDS             complement(18877..19035)
                     /locus_tag="PWS53_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870756.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(19176..19800)
                     /locus_tag="PWS53_000485"
                     /pseudo
     CDS             complement(19176..19800)
                     /locus_tag="PWS53_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19797..20108)
                     /locus_tag="PWS53_000486"
     CDS             complement(19797..20108)
                     /locus_tag="PWS53_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870757.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20152..20445)
                     /locus_tag="PWS53_000487"
     CDS             complement(20152..20445)
                     /locus_tag="PWS53_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870758.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20638..21285
                     /locus_tag="PWS53_000488"
     CDS             20638..21285
                     /locus_tag="PWS53_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870759.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21346..22491
                     /locus_tag="PWS53_000489"
     CDS             21346..22491
                     /locus_tag="PWS53_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0870760.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22572..22877)
                     /locus_tag="PWS53_000490"
     CDS             complement(22572..22877)
                     /locus_tag="PWS53_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL0870761.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22902..23333)
                     /gene="ruvX"
                     /locus_tag="PWS53_000491"
     CDS             complement(22902..23333)
                     /gene="ruvX"
                     /locus_tag="PWS53_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL0870762.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23330..23596)
                     /locus_tag="PWS53_000492"
     CDS             complement(23330..23596)
                     /locus_tag="PWS53_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL0870763.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23851..24576)
                     /locus_tag="PWS53_000493"
     CDS             complement(23851..24576)
                     /locus_tag="PWS53_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0870764.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24937..25007)
                     /locus_tag="PWS53_000494"
     tRNA            complement(24937..25007)
                     /locus_tag="PWS53_000494"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24973..24975),aa:Gly,seq:ccc)
     gene            25124..25876
                     /locus_tag="PWS53_000495"
     CDS             25124..25876
                     /locus_tag="PWS53_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0870765.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25892..26371
                     /locus_tag="PWS53_000496"
     CDS             25892..26371
                     /locus_tag="PWS53_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0870766.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26622..28202)
                     /locus_tag="PWS53_000497"
     CDS             complement(26622..28202)
                     /locus_tag="PWS53_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL0870767.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28195..28938)
                     /locus_tag="PWS53_000498"
     CDS             complement(28195..28938)
                     /locus_tag="PWS53_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870768.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29114..29890)
                     /locus_tag="PWS53_000499"
     CDS             complement(29114..29890)
                     /locus_tag="PWS53_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0870769.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30138..30974)
                     /locus_tag="PWS53_000500"
     CDS             complement(30138..30974)
                     /locus_tag="PWS53_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL0870770.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30985..31914)
                     /locus_tag="PWS53_000501"
     CDS             complement(30985..31914)
                     /locus_tag="PWS53_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL0870771.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            32106..32459
                     /locus_tag="PWS53_000502"
     CDS             32106..32459
                     /locus_tag="PWS53_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0870772.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32533..33189)
                     /locus_tag="PWS53_000503"
     CDS             complement(32533..33189)
                     /locus_tag="PWS53_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0870773.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33415..35349
                     /locus_tag="PWS53_000504"
     CDS             33415..35349
                     /locus_tag="PWS53_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0870774.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35435..36658)
                     /locus_tag="PWS53_000505"
     CDS             complement(35435..36658)
                     /locus_tag="PWS53_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0870775.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36652..38550)
                     /locus_tag="PWS53_000506"
     CDS             complement(36652..38550)
                     /locus_tag="PWS53_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL0870776.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(38544..39662)
                     /locus_tag="PWS53_000507"
     CDS             complement(38544..39662)
                     /locus_tag="PWS53_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0870777.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39745..40578)
                     /locus_tag="PWS53_000508"
     CDS             complement(39745..40578)
                     /locus_tag="PWS53_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0870778.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40571..41488)
                     /locus_tag="PWS53_000509"
     CDS             complement(40571..41488)
                     /locus_tag="PWS53_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870779.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41715..42839)
                     /locus_tag="PWS53_000510"
     CDS             complement(41715..42839)
                     /locus_tag="PWS53_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0870780.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            43022..>43874
                     /locus_tag="PWS53_000511"
     CDS             43022..>43874
                     /locus_tag="PWS53_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870781.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSS"
BASE COUNT        13208 a         8833 c         7246 g        14587 t
ORIGIN      
        1 attcttcctt ttttattaag tatgaaaaaa tatttcaatt ttattttttc gtttcaaatt
       61 attctattag tgacatcctt gaactataaa aataggagta cggcatcatc tgccgcactc
      121 ctattttttt cgtcatttat ttcttaaata aaacgattct cacgcaattt tatttttttg
      181 atatcttcta ctgtttgtgt tccagctaat tgcatgacca tttccaattc tgttttaaag
      241 aaatcaaaca cttgatggac tcctgtactt cctcctaaag acaatccata aataacgggt
      301 ctgccaatag caacaagatc ggcacctgat gcgattgctt tgaaaacatg ttgtccacgt
      361 cgtacaccgc tgtcaaacac gattggtaca cgtttatcta ctgcttctgc cacatattgc
      421 aacgaatcaa acgcagcagg ccccccatct aattgacggc caccatggtt agatacccaa
      481 attccaccag caccagattc taatgatcga tagacatctt cttctgattg aacacctttc
      541 acgtagactg gcaaatcaga atgagcagcg ataaattcta catcttttgg actaagtttt
      601 tgtttggaag atttatatac agcatccatc gtttggccta ctcctgattg ataagcctga
      661 acgatcggca ttggcagcgg gaaggtaaac ccgtttctgc ggtctgtttc acggtttccg
      721 ccaactgttg catccgctgt caatacgatt gcttttgctc cattacgctt ggccacttca
      781 agaatatcca aattgattcc attatctttg ctcatataaa attgaaacca ttgcggtgct
      841 ttctccccgc ctgcttctct tatctcttct aaagtacaag aagcgtaaga acttgctgta
      901 tagatcgtcc caaaatctgc tacacctttt gcagaagctt tctccgcctt cacatgagct
      961 aatccatgag cagcaactgg tgccatgatg attggtgcag tcagcatttc ttcgtcaaaa
     1021 tgtgtagttg tatcgggtaa ttctacatct cttaatacat gaggaataat caaacgatga
     1081 ttgaatgccc gttcattttc ttgataagta aataagtcgc cagcaccact gctgatgtaa
     1141 ccataaccac cagctggaat cacttgtgaa gccgcgattt ctaaatcttc catattgata
     1201 aaatctacta ttccttcatt tgtacctgct tgatatgttt tttccatctc aatgccttct
     1261 ctctataaaa tttgaatgat gaacaatatg atagcgttta cactttttat attactcttc
     1321 gacattgata caggcaaata atctgtttac atttcagcca ttccagatgt cactactctg
     1381 gaagaaatga ttgtaataaa aatagataaa aaaccgatgg gactttgaca aaagtagtgt
     1441 caaagtccct ttttttgaat aaacggtgtt caaaagccat tttctcggta ttaagtcaaa
     1501 tttatgcaaa aatgtggaaa gccattttca caaaattttt cttactactc gaaactaagc
     1561 gactttttca caacctctcg ataaaacggt gttcaatcag ctgaccctcg gtattagctc
     1621 aaaaaatggc aaaatatgag gaactatttt gccatttgtc tctctaatac tcaggtcaga
     1681 acgttgactt cacagtctct tccttaaacg gtgttcaaaa acttgcatct gcggtaataa
     1741 gcccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
     1801 cagggcaggt ttttcacgac ctctgattca cggtgtttct ttttcatcaa catttttacg
     1861 aaatactgtg gaagactatt gaaataagtg tggattgttt ctttgtgttt caatcgtttc
     1921 ttgccattct tcatttaatt ttggattacc gttttcttcc ttgaactcat aaggtcctaa
     1981 ttcaaaaatc ccagacagta tttgattcac gatttcatat agttcagtgt gatcagacac
     2041 atgattcttg aagatcatct cattatcacc acgtttgaac ctcacattcg ggctctcatg
     2101 atgaacctct atgcattttt ctcctttttt taactgacgg acttctgaaa acatctcata
     2161 aatatcttct ccagtaaaac cttcagcaag cgccagactg tccacaagac cgcctactgt
     2221 cagttcagag attattgctt gattggaatc taattttaat gtatagcttg tcgcttctac
     2281 tacgccacat gcattgcaac cgccttctga tagttcgctt tttctaatca attgaacttc
     2341 tttcattttc tcttcctcct cttaattttc tacttatgta tttttatcac ctgacatagg
     2401 tgactcctat caacgctatc atgtgctttt ttcctcagaa atcctattta tccattccat
     2461 ctatccctta ttcttctact tttctttctc tagcatataa gagatacgca tagtattcaa
     2521 gcaaggattg tcgatataaa aagaacaaat actgctattt tgatttcttt tggctatatt
     2581 acatataaat aaaatttatc atacctgctc tttttgacta tatcatcatc tgacttctat
     2641 acttcgtttg tattaaaact taggaggaat cattcatgct tacagggtta aacttattaa
     2701 ataatccttt tctaaataaa ggaacagctt ttacaaaaga agaacgggca aaatatggaa
     2761 tcactggtat gcttccaagt actgtccaaa cactggaaca acaatctgtt caagcttatg
     2821 gtcaatactt aagcaaacaa acagatttgg aaaaacgtat cttcttaatg aatctattca
     2881 atacgaatcg gacattgttt tataaattga tgggcgaaca tttagttgaa ttcatgcctg
     2941 tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata caacgaaatt ttcttgaaac
     3001 cgcaagatgc tgcatttctt tcaatcgacg aaccagaaaa catcaaagca agtttgaaga
     3061 acgcggcaga tggacgagac attcgtttga ttgttgtgac tgatgcagaa ggaatcttag
     3121 gtatgggcga ctggggtgtg aacggtgttg acatcgctat cggcaaattg atggtctata
     3181 ctgctgctgc tggtattaat ccagcacaag ttttgcctgt atcgatcgac gctggtacta
     3241 ataatgaaga attattgaat aatcccttat acttaggcaa tcgtcatgct cgtgtagaag
     3301 gcgaaactta ctatgaattc atcgatcaat tcgttcaatc agctaccgaa ttattcccag
     3361 aattgttatt gcattgggaa gattttggac gaggaaatgc tgcagcgatc ttagaaaaat
     3421 acgaagatca aatcactact ttcaacgacg atatccaagg tacaggaatt gttgtcctag
     3481 caggtgtatt aggcggattg aatatatccg gtgaacaatt gaaagatcaa ataattttga
     3541 catttggtgc tggtactgct ggtgtgggta tcgcaaacat ccttttagat gagatgatcc
     3601 gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta tcaagtagat aaacaaggtt
     3661 tattgtttga agatacagaa ggattgactc ctggtcaaat cccattcgct cgcaaacgct
     3721 cagatttcac taatgctgat gaattgacaa acttagaagc agttgtaaaa gcgatccatc
     3781 cgacaatcat gattggtact tctacccaac ctggagcatt cactgaagaa atcgtgaaag
     3841 aaatggctgc tcatacacca cgtccgatca tcatgccttt gtctaatcca acaaaattgg
     3901 cagaagcaaa agcaaaagat ttaatagaat ggacggacgg aaaagcatta gttggaacag
     3961 gtatccctgc tgctgatgtt gaatataatg gtgtaactta ccaaatcggt caagcaaata
     4021 atgctttgat gtacccaggt ttaggacttg gtttgattgc ttcaacagct acacgagtaa
     4081 atgctgaaat catttctcaa gctagtcgag cattaggcgg aatcgttgat gtaacaaaac
     4141 caggagcagc tatcttgcct ccagtagcaa aaatcactga attttctcaa acgattgctg
     4201 aaactgtcgc aaaatcagtt gtcgcacaaa acttgaatcg tgaagaaatc acggatatca
     4261 aagaagcagt tgaatctgct aaatgggttc ctgaatataa atctttagaa gattaagatc
     4321 gttatagtaa aagaacatac agaaactttg tatgacgtct aaaaagaaaa aagcgactgc
     4381 ggcaaaacag cggccagcca actcctgctc ttttgtcgct tttctttggc ttttttcata
     4441 tgatacagaa taagaaagga tctgtaaaaa tggcacaaaa aacaacaact gcaactgcag
     4501 ttgaggaaaa gaaaaaagaa ggtttttggt ccacaaaaat caccggcgta agtttgccac
     4561 tttatttgat catggtcctt gtcctgatca ttgcaatggc tttagggaaa ttaccgacga
     4621 atatgattgg accaatcgca gttttggtta ttttcggtaa tttatttcat tttatcggga
     4681 caaaaatccc tatcgtcaaa agttatttgg gtggtggttc cgtttttgcg atcttcgctt
     4741 ctgctgcgat tgctacattt gggatcttac ctgaatatgt agtgaaatcc acagaaaact
     4801 ttgtcagcaa tatgggcttt tgggactttt atattgctgc cttgattact ggtagcatcc
     4861 ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg ttttattccc gttgcactta
     4921 tttcaatggt cgtctccttt tttgccgtag gactcgtggg catgcttatc ggaaatggtt
     4981 ttgcaaattc cgttttatac atctcattac ctgcgatggc tggtggtgtt ggagccggag
     5041 ccgttccttt atcaacgatt tatgcaaatg ttttaggtac ggatgcttct tctatcattt
     5101 cccgcctgat tcctgcgaca gcaatgacaa atgtcttagc tatcatcggt gcagcattag
     5161 ttgctcgtgc tggacaatca atgccaaaat tcaacggcaa tggtaaatta atggaaaaag
     5221 gcgatcttgg cgatggaaaa cctcgaactg tgaaaccttc cattccacag ttaggtgttg
     5281 gtttgatggt atcattgact ttcttcatct taggacaaat tggtaactat tttgttccta
     5341 aagtccatgc ttacgcattt atgattatta tcgttgtcat ctgtaaaatc gccaacctat
     5401 taccagagta ttatgaagat gcagctatca tgttcaataa cttgattgtc aaaaacttga
     5461 cagcagctgt tcttgccggt atcggtattg cgttgctgaa tctgaatgta ttagcttctg
     5521 ctttgacatg gcaatttgtt gtcttatgtc tgacaagtgt tatcgcgatt tcagttgttt
     5581 caggatttgt cggtcgtttg tttggtttat atccaattga atcaactatt actgctggct
     5641 tatgcaacaa ctcgatgggc ggaacaggaa atgtagccgt cttatctgca gctaatcgga
     5701 tggaacttat tgcttttgct caaatgggca atcgacttgg tggtgccatc gttttgatca
     5761 tctctggttt cttgatgcaa ttactttctt agtcaaaaaa acaaaaatac ctgatcatca
     5821 agtatcggct tggtgatcgg gtattttttt gataaagatt tacaggattt tcaaaaatct
     5881 tttttgcatt ttatcggaat gataaccgat gtgttcataa aattggtgtg cttctactct
     5941 gtattctgca gaattaagcc ttattgtcgt tattcctcgt tttttcgctt cttcttctac
     6001 gccagtcatc aaagcctttc cgatgcctct tttttcagca tatttagaca cagcaagtgc
     6061 cataatattg aacattggat cgctaaaaat cgattcatag gactccgctt gtacatagcc
     6121 agctgctttt ttagaagtag gatcttcata aaccaagaaa aaatttttct ctggctcctt
     6181 cagcagtttc ttcatttgtc tcaccgtaac ggttaatggt atatcatacc ctaattgatc
     6241 cgcattgatt tcacgtagtg cttctgcatc ttctaaacgc atttcccgta acatccaaat
     6301 caccagccaa tttctgattt tttaaaatga atttttttat catagtatac tctttttttc
     6361 atttagaaca atatgtttca gtgattttga cttgcgttag tctagattct cctaatgttt
     6421 gtaagatgac aaaggcattg taacaattga ccaaaagaac cacgaggaca attacttgaa
     6481 aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc
     6541 caataacagc agcggggata acataaaaca acatagataa atcaaagcgc ataaagctac
     6601 ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta gatagagaat
     6661 atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa acattgactg
     6721 gtccaccacc aatacctaaa aaactagcta aaaagcccag aacccccccc acatataagg
     6781 taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta gaagaacgca
     6841 aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt ctcgttttca
     6901 aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac aacagctcca
     6961 ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac ttgtcgcaca
     7021 gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat ccctgcaaca
     7081 gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc catcccggaa
     7141 atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc aatcatcttg
     7201 tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt tattatacag
     7261 taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt tagtgctaaa
     7321 cgaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     7381 agctcctcat aattttcgtt tggttgaact tattaccgca gatgcaagct tttgaacacc
     7441 gtttaagaaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
     7501 aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac
     7561 accgtttaag gaagagactg tgaagtcaac gttttgacct gattattaga gaaacaaatg
     7621 gcaaaatagt tcttcatatt tcgccgtttt tcgagcgaat gccgaggggc agctgattga
     7681 acatcgttta tttggaaaaa gggattgtga caagacgttt gtcacaatcc ctttttttat
     7741 atcttattaa ttcattcttt tgttctattc ttcttcttcc gccatgaatg agaaacccat
     7801 atcaaagaac tataaaggtt atcaaaacag cgtttatgac taaatgaatt agagttatct
     7861 atcactattt ttcaagcgat ttaaaccaaa tggcatacaa tatggcatac aaaaacttca
     7921 ataagccgtg ggggttttta aatttcttct tgttctttaa aatcaatgag tggagctaca
     7981 taatttgaat ccactttttc aaacgtcact ttttgcatcg acgaatgttt tctttcccaa
     8041 gataacgtaa atagatttga ctctggactt ataaagtcta cttcaccttc tagtattact
     8101 ttcttatgca aatattcctc taataaatat agacttgcta aagcatacaa cacattttcc
     8161 aaatttgctt ccttataatt tgaagaacga ccatgtttta ctctgttata tattttccac
     8221 caatctggtg tatcttcagg ccacccatct ccaaaaggtt ttagtgaagt caatgttaaa
     8281 gaattcataa ccctaacacc attatccaaa cacattaatt cgctagattt aataacagct
     8341 ttaaaatcag ttatgctttt attgctactg ctatcaaatc cgcaaagttc tttgaaaata
     8401 atctcacact ccgaaccaat cgctagtagt agtttttgat attcaataga gaatgttttg
     8461 aaattattct catcgagcat cacaaaatct gtggtattaa taaactcatt ttctaaatgt
     8521 agataatact tccaataaaa atttgtaaac tctgctttgt ccattaaatc acctcttttg
     8581 aattataaca aaaaaaacac gtccacaaca gacgtgttct ttgatttcta cacaatcagc
     8641 ttgccaaaac catccaatca gtcctagcat gcctgtatta aaattgaacg ttcatcatct
     8701 atcatctttc ttagtccagc atgtttgcaa ccacacctac aattttcagt ccaacaatag
     8761 atttcgacta gctaagtctt gcaaaattca ggcagctaac ccttcattta ctaccttaat
     8821 tatactaaat acccacaacg tttacaagtg taatcattct aagaaatctg tttagctatg
     8881 atcgttgtat aatctttaaa cgccgttcct tcattgattg aaataatttt ataactcttg
     8941 tttttccaag ctactttcat atcgtttgtt agttcataat cttgatcgta tcgaataata
     9001 aacgtcaacg ttccttcaag aatggtacct acactagcaa gcacgtcttt tagcatttgt
     9061 gaacgcacat tagcccaaca ttcaaataca gtttcttcac tatccaccat ctgcccatct
     9121 tcatctttta cccgttttgt ctttttaaac ttgactctct cagtcaattc atttacattc
     9181 ttaatgattg gcataataag cacctctcat ttgttgtact aatgcttgta ccgttactgg
     9241 tatccgttcg ctgctggctt cttgacggtt caaataccaa tgttgagtaa gtaatgacac
     9301 cgccaaatca aattgctgat aattatccat atctttatcc gttgcgctac tatcaatagc
     9361 actttttata tattcgcctg ctgtatcaat caattgttga ataaagtcat catcaatagt
     9421 gtgatcgatc cgcatacttt tttttatgct atccaaaatt accattttat ctctcctaat
     9481 gaaaaaaagg cagtgggata aaccgccgcc caatattttt ctatactttt ggtgttacaa
     9541 ctggcgtaaa ctcaatatac actgaagcat tttgatcaat tttcttataa tcgttgcgaa
     9601 cgatcactgc aagtccttgt gagtagtaat cgaatttttc ccattgagtc gttacttgat
     9661 ttctacgagc aacaaagata gattgtgcaa tatcccccat aatcataggg aatgttccag
     9721 ctttaggatt ggcaaacaat gtatctgcaa tcaatactac tggcataccg aataatgatt
     9781 taccactagg tgctgtcaca tcgggttgta aaatgtaacg tccatctgaa tctttcaatg
     9841 tatctaggtg gtaatatccg ctttggttta gaattaccat tttatttaat gctgggtcta
     9901 atgccacatt gtatagttgt ttcaaatcgt ccaacgtagc agccgttttc ttagtgaatg
     9961 tttttaataa atccatgata tgcttattgt cagtgttttc taccaatttc gctaactgat
    10021 ctttgacctc ttgtacaata ttcactgctg aatcttccac aacctcattt gataaggcaa
    10081 tcttaccagc acgagtttct actttataat caactgaagt aaacatttca gcgtcaatat
    10141 caccaatttc agcaagttca gcttttgttg ctaacactgc ttgttggtta gttgctactg
    10201 gatatttacc ttgtccattt gatactgttt ttactgttgc atattgagct aagttataat
    10261 ttgaacgttt caaatcaaag acttcaccga ttacttcttc gggaacaact gccgctgcat
    10321 ttacagtagt gactccatca cgcacttcgc cttgtgaacg gatatattct tcatacccac
    10381 gcaattctgt tttttcgtta tcgattaaag ttttttccat gttattactc tccttttgtg
    10441 ctttagaagt ggattctaag ctattgtttg tttgttttgc ttggttgtta ctcataaacg
    10501 attcgtaaga acgcttattt acttggacat ttgatgaatc ataagcggga acggtcacga
    10561 cactaatttc attaagtgct ttgattttat ctattgatcg ttcaattgtg ccatcttctt
    10621 ttttgtcgaa tgaatctaat ccaagcacga acccaaagga catagaatca acaacccctt
    10681 ttgagatatt ctcatacaca tcatttgaat aggtcgtgtc ggttagctct gcttcaaaat
    10741 gtaatcctgt atcatcgaca ttcaatttca acgtacctgc tttgacacta gctagtggct
    10801 ttgaataatc gtgtccatat aataagaaca catttgataa atccacctct tttaatgctt
    10861 cgggtgtaat cacttctaca aagccgccta aatcttttga cggttgcccg aatttcaacg
    10921 catagcctga aatagttttg ccgtctgttg gttgtttttc attttctttc tctactttgg
    10981 gagaatcggc tgttagctca gcttcttctg tcagccgttt ttccttttct tcttcattat
    11041 tcaactggtg taactccttc ctgttctttt aattgatatt tctccaacgt atctaaatac
    11101 gtaaagttca aacttgctag taaacgatcg ccaccatcca tggggggtag ccccattttc
    11161 gaccgcccct cattaatcgt gagaagtgac cctttgacct gatccaatac attttttact
    11221 tttgtttcag gatcagtctc tagtaactga tcggaattaa agcggatatt ggtttcaagt
    11281 ttcgtatcta attcactcac aaacacatta aagtaatgaa tcagcgtact ttggatatac
    11341 tggagattac tttgaacggt gctagaatgt tcattttcaa cgcctaaacg ctcaatcggt
    11401 acaccaaacg ccttggcaat ttgttttgtg ttccaatcat tggaattgat aagcttcaaa
    11461 acatcggtgt ttatttctaa tgttttgtag tccattgttt catcaagaat gatcgttcta
    11521 agagcattat ctccactact tgaaccgttg gcttcttcaa acttttcacg aattgcgttc
    11581 tttgctgatc cgtctaaatc tgacttatga accttcaaaa tacctgaccc actgacacca
    11641 cgagtaaaga aattgtgcaa tgtgcggttg ccagcttgtt gaattttcag ttcatcacgt
    11701 aacgcataaa gtggtggtaa tcctgttaaa ccatcttggg tgaaatattt gaagtgcaaa
    11761 atatcgctag acttcacacg tcttttttta tcaccaatct gataagacaa cgtaccatta
    11821 tctaattgag taaccgttac acttgagtta ggtaataagt gaatttcttc taccttttca
    11881 ccgttgcgtt taatctctgc aaagctatta ccgtttagca acatattgac cgccaaagca
    11941 aactttaaat gccaaccatc catttctgaa ttaggtttct cgtttagtaa cttcaccagt
    12001 tcattatcag cttgtggcat attgtttttg accaattgaa tcggacttga agcaatatca
    12061 ctagcaataa ttcgaacggc tgcaaacaca tcactatttc ttaatgcacg aacgcttgta
    12121 aagctggtgg tgtaatcatc cgattggatt gataccacat gttgtaggaa aggatctccc
    12181 gttgtatttt gtctcgcttt aaaaaatgcc atcttttaac ctcccttctg ttctgtattt
    12241 agcatgaccg ctaccccaat aagcactatg cccagcgcta acaagccata gtagacattt
    12301 gttagtaagg tgatagcaac caacacacac accaatccaa gaagtaaaag aacggtatga
    12361 atgtaattag aaagaaaatt cttcacttgt ataaaattca ttgtctgctt tcgctccttc
    12421 cgttgtggtg aagtaatcca ttgcaaacac ataggcattg attaatgcag ctattggatc
    12481 aattttgtta ctgttctttg atttattaat ctgaatgccg ttgttatccg tttttaaaat
    12541 agcgttgttc actgcatggg tgagaatcgt atttttattg tgaataacgt tgccttcata
    12601 aacttgttct ctaaaagttc gggtcggaac gtttagagta atcgttcctt gtcttacttc
    12661 taacattggg tagttggctt tttcagcttt actgataagc gaattagcgt tatacgggtc
    12721 ataacaaatc cccatacatt ccaaatcatt ttcttgaatc aaatcttgaa taaactgaaa
    12781 tacttggtca tagtccacga ttccactttc taattgagta atggaacact caccagcttt
    12841 ttctagttct cgataaggca aaccgtcgcg cttttctttg tcttgaagcc catatttggt
    12901 ggctacaaag ctatgtgaat cacaataaag ttcgccgtta tctagcggta cgatccatga
    12961 aacgctagtt aaatcatctg ttttagataa atccactccg atataaacgg gcttgcctgt
    13021 aatatcttgt ggttctactt cggttgcctg ccagtcttca gcaatcatat agctgtcctc
    13081 actcgcttgt ctccataaat tgaagttttt caccaataca gaattaaggt tattttgttt
    13141 caaagcaagg ttcacatcat cttgaatggt tggaatcatt acttttttga tttcttcact
    13201 ttcaaaaatc ggattggctt tgatccattt ctcttgatcg tgaatttctt cgggatcgtc
    13261 caattcccat atcgctataa agtacctgtc ggcttgttct tcgcctttta gcacacgatc
    13321 taacaataag tattcctcgt acataggaac gtttaaatca agcccagcgg tactaataac
    13381 acacaataag ccgttctttt gttgggtcat tcctgactta atgacgttgt acgttttacg
    13441 agtttttgct tcgtgccact cgtcaagaat tgcggttgtt ggtgcaaacc catctagcgt
    13501 gctggtttca ctagccagtg ccatagcgaa cgaattagag ggcaagtgct gaatcttaga
    13561 gttcataatt ttcagttgtg gtcttaaaaa cttactagac ttgaccacgt ttctaagtga
    13621 attagacagc atatcatagc ccaatttagc ttgtttcaaa gcgttactta caaacaatac
    13681 ttgccttgct tctgctggtt cttgttccat aatcaacgca ttggcagcca tgccacttgc
    13741 gatatacgtt ttcccattct tacggctcat actggtaaac gctcgattga atcgcctgta
    13801 attcccttct ttagtacgcc agccataaag actgcctaga atgaattttt gaaatcctaa
    13861 catcttgatt gctttaccgt ccgtagatgg tagcaactca atgaatttaa tcgctttggt
    13921 ggcttgttct tcatcgaaca cataagggaa gctatctgac ttagaacgct ccaaatcgct
    13981 tatatgacgc tcacacgctt ggataatttt cttgggtgca atcagcttac ctgataggac
    14041 tttctcaatg taatcattca tgaattcatc aactctttga acgggtcttc gggttcatca
    14101 tttttaacct tgttcaaagc tagttttgat cgtgattcta aggtcatacc tagtgaggtg
    14161 gcttgttttt tcaaatcact catagcttgt gattgaaccc gaaccgctgg attggctttc
    14221 tttactccgt tttcgcatgt gataaacgta cctgattttc ttatctcggc ttcggctgtt
    14281 ttcattcgag caaaggctaa acaataggct accaatgaac cataatctgc ttcactcaat
    14341 ggataatctt tttttaatgt tggtacgata cgattccatt cacttctagc gctcgctgcc
    14401 atccagtcag ggggcgtgat atttattaat ggttgctgct tgaacaattc ttctctagcg
    14461 tccacacgga ttgctttttc ttcacttgat atattgcctt ttgtatcgtg taatatttta
    14521 gggtgtccca tgttgtcctc ctttcagaca aaagaaaaag gaacgtaagc aattaagctc
    14581 acattccttc gtttttcgat cagaattttt ctttatctaa tttatctata taaatgattt
    14641 ttccgttctg catttgaatg gtcacttgac caaaatgagg taactcaatg gtgcgctgtt
    14701 tccctgtttt atcagtaata acaattgtat ttttcaataa atctcctact ttcattatat
    14761 actttttcac ttgtgattac aagtgtaatc gttatttgta attaccctta tgccttttca
    14821 caacggttat ttgtaccaaa atgagtaggc atcgattgtc agtaagtttc acactatgcc
    14881 cccttaactc aaagggtact cttttctcgt ctttgtcttt cattggctgt cttggaatta
    14941 tggtgtgcat ggcataaaga acgaaaatta gtttcatcta accgtctatt ccaatcatct
    15001 tttagttcaa ttttgtgatc tacaacatca gctttcctta tcaagccttc ttctaggcaa
    15061 tcctcacata ctggatgatt taaccgatac aagtatgaag cctttcgcca tgttctactt
    15121 tgatagaatt gaaaatattt accttcgctt tgctttcttt cttcacgctt aattgccgtt
    15181 tgctttgctt tatgcttccc acaatacttc tgcctataat caatcaatgt tttacatcca
    15241 gcatgattac attgtttttt aactgccata gattaccaac caaagcgatc tcgttcgtca
    15301 gaatcatctt gttcccattt tgatttgtat tcaatataag gcgaaactat cttcaagcac
    15361 gttccgtctt taaaattaac tttagtgatt ttatcttcct caaaactaat aaagtctaca
    15421 tcttcttgag tgtttgaata ctcaatgtgt ttctcaccat cgaagtatct aatactttcc
    15481 atatgcataa ttattctacc tccaataaat tagttatcaa ttcatcatct gttagtagca
    15541 aagccaagaa cttatcatgg atgtttaaaa acaaattatc ctcattcgat gtaagctctc
    15601 gttgtctcgc tcgatcatat aggttaagca gacaattaaa ttcatccatt agctcacctg
    15661 tatcatcata gttgccacta tctaccaatg atcccactac tagcgattga caccacgcta
    15721 ctgactcctg cagccaatca ggttcttctt ccattatctt taatacttct ggatcaaatg
    15781 tggaataatc ttctgttacc aatcccacac taaatgccag cgtgttcatt agaaaccgat
    15841 ccatttcatc tgtatattga aagttcggtg ctttgtttgt tgaaataata ttgagcattt
    15901 actttcctcc ttgattcgta acacgtggta acacctacgt aacaggtacc cgttaccact
    15961 caatcccaac ggggacaagg gttctagcgt tttggtaaca ggtaacgggt aaaatccagt
    16021 aacattattc cccagcatat tagctaacta tttcaaaagt tttaaggaaa taaggtgtta
    16081 ccccgttacc aaattgctgt atcccttggg gcagtaaggg taaactagta acgggctagg
    16141 tgttactgac ccgttactac ccgttacttt ttgtactttc cagctcgatt tttattttca
    16201 taggcatttg acttattgta aaatcctcgt cgtttatttc tttttggcag ttgcttccgt
    16261 tcccaaccag gaatactgct aataatcaag gcaatctttt tagtttctgc atctgatcga
    16321 ccattcaata aatcctttgc ttgtttatca aaaacagctt ccaaaatatc agcggtgagt
    16381 acgctatcaa ctaaataaaa ctcttgttgc atagtagctc cattatagaa atgctgtaaa
    16441 tttttctcat tacatttact gtcaggatag ttctgataat aagaacgtct cgcccacgct
    16501 ggagcttctt cccatttata cgggatttcc atttccaaat atttagtgat tgcttcttta
    16561 attggattct ccatcttggc atcctcttga tagtcttttg caatttctaa gacttcttta
    16621 tcttcatcgg gactaaaata gattcgttgt cctttatcaa aaagcacctt tgcttcagct
    16681 aacacttgaa gaaaatattc atcctttgtt ttaaacggat tcatctttgg cttattctta
    16741 caaggcaaag gataaaaacg gcggtttcct gtatcatctt tcaaatactg cccatcattc
    16801 gacgttccaa taaatacaca ttgccttgcc caagctatga cattccgacc ataaggaggt
    16861 ctaattttat cgatcttggc actgatgaag ttttttatct gttcaatttt agttttgttc
    16921 attgaagata attctccaag ttcaataatt acattgcctt gaagctgcat ataatcatct
    16981 ttcttttcac ctagcgaatc taatgaatcg gtgaaaaaat catccgtgta aagtaatgct
    17041 ggaacggtac tcttaccaat cccttgcggt ccatccaaaa ctggaacgat ctcaaacttt
    17101 attcctggtt tatagatacg tgcgactgat ccgactatcc aacgttttgt tacttctcgt
    17161 gtgtaaaggt tgtcgtctac ccctaaaagg tcaataaaca gtgtttccag tctaggctgt
    17221 tgatcccatt taactttttc gatacgttct tttactgggt tgaaaacatt ctttctagcc
    17281 acatttacta ctgctttgtg tattttgttg tcatcaaaaa ctacattgta gttttcttca
    17341 atatatgccg ttaaggcatc ctcaaaactg tcttcccatt ctccagcggt acgattgatc
    17401 caaggagaat cttttaatag gtgaatgtat ccgctaaatt cgttataagc aattgttcca
    17461 gctaaattgc tatcattggt taaaatcaat tctgcattca caacactgtt acttttaatc
    17521 tgatcgtctt tattgcatct taatcgtgac atccaggttt tcaattcaat gatattgtct
    17581 tcacgctcgc tggcttcggg aaagtctaaa atactatcta atttatgctt atgagccatg
    17641 agctttcccc ctcaaatctt ttttaatcat cgatcgaata atttgattca cttctttatc
    17701 ttttagcggt ggttcattgt acgcatttgc tgtataaatc aattctgcta aagtttgatt
    17761 atctacaccg tgccatagta accaaccgca taacttagtt aaatgatcgt ttctactacc
    17821 ttcgccagcg ccttgaacaa tcatatctaa tttttttgct gtccctgttt ttgctacggg
    17881 tcggtattga gtctttattt cttgcggttt ttttagcagt aggttcacta gccaatcggg
    17941 agctggtgca atattattta gagatacgtt ttcaataggt tcatatgctc tcccaacact
    18001 tcttgaaata atcatagttg gcgctattgg aactccttta gtgataatat caatcccgct
    18061 taaactttta cctttatcat cacgcccgaa gatatcagca aatgcacctg tttcttgttg
    18121 gataattaca ccatcaggaa ttttaaagaa gaaatggatt cctttacctg gtgtgatttc
    18181 cgtataagta gaaggaaatg gatcatacat tgaagcaagt tttttataat tctcaacgcc
    18241 gtttcgtgtt tcagaatgtt gatctaggtc aactaccacc atttgatgat cttcaagcat
    18301 taacccaata tttctagttg catcttccgt ccaccattca ttaatttgat tagaatcgtt
    18361 agttgcatca ttccaaccac tagagccttt tattggaatt ttagttccag gagataaggg
    18421 ataaattgac catcccgact tttgtaattc cgttgctgct tgataattat ttgccaataa
    18481 acctctcctc ctttttcaaa atactggtat aaatcattca gacacctcca tgagcttcaa
    18541 ctgctcaagt tcgtatttca acccatcccg aacaaattca gcacctttta attgattctg
    18601 atactttacc catgtattaa atgttttcac ccttgcttct tgcatatcag taatgtttaa
    18661 ccatgcggtt aaaatctgat tttttagctc gttgatttct cgacaaatta ttttgtgttc
    18721 tgcatgtttt ttgagtacat acaaaactgt tagtttttca ttgaatgaat ttccaaagta
    18781 ggttttcccg atattatttt ctagttcagc gatcccttga ttaatttcct ctaattcaag
    18841 gctatcgagt tggtttaata gttttgtttt gtacatctac ttatgccctc cagttcttaa
    18901 ttgatagtcg tactcgtcat aagcagtgaa gagatataac gctaatcctg caaagatagc
    18961 tatttttgca attaatggta ataagccgaa aattccgccg attaaaatgc ttcctataat
    19021 taagtttcgt ttcataatgg ttccctccga ttttttaata tggtataatc ggaacaaaga
    19081 atatcgggtt ttatcccaat taagttgcct ttatcctgtc cgccaagact gttatggata
    19141 aggctttttc tttgttctcg atcatgttaa tgctcctaag ttgtctgcaa ttcagcagaa
    19201 cgattataaa ttttctcact cactgaaaac tgcaacccat atttttcttt aacagtcatt
    19261 aattctactg tctcatctaa aataggttca cgatcaacca acatttcagg agtcatatct
    19321 ttctttttta ccattttgga atgaccataa atagttgata cagctttatt ggctatcaca
    19381 ttcgctttaa tgtagtctac tggttttggt tcagtaatag cgtggcttaa tttagtcatt
    19441 gcttcttttt ggtgttcttt gtctagcatg cggaatactt gaaagccttc aagtcctgtg
    19501 gactggcgta gttctttgat gatgtcgaaa atccagtctt gaaatgcctc ggcttctttt
    19561 ttacggcttt tgaatgccat gcggtaaata ttttttttcg ttgatgatgt ttacatcttg
    19621 tgttctgccc aatgaatcga tgaccttact agtagtaacc ccatctttag gcaaagagtg
    19681 aatggctcta gtaacctgtt taagacctaa cgcttccgca acatctttcg ccactgccca
    19741 ccattcatca ttaatatcga caaatcttat aatgtgtccg ttccaaattt cagttttcat
    19801 tcaacgtcct ccagttcaag ccctagcaag ctcgctaaca actcaacttg ctgatacaaa
    19861 atttcttgat actcttcaat agaaagtcgt tctttaaact ctgcttgtac taaatcgttc
    19921 aagccacgca atactccgct taccgttggt tcttgtggta gcaatttttg atagtccatt
    19981 tgttcgtaca aacattcaaa cgtttcttct agcgctttgc cttccaagtt tgctaattct
    20041 gccgctgtgt cttctgtaag aattgttgat aatacttttt cgattgcttt attttgtgtc
    20101 gattccatta ttatttcctc ctattaatcc ctttgccccc acggcttgcg tttatttttg
    20161 gtattcttcc ataaagcgat cacattcatc ttgtgaaatt cttacgactc catcaactat
    20221 aatcaacttt aaaccgttag gaacatattt ctgcatcata gtattgtatg aaacgttcat
    20281 atacttagca gcttgtgcct tgttcatata ttttggaaac tggtactttt tttctaataa
    20341 tgaaagtaac gttccttgag tttgctctac ttgttgaaca actcgatcgt caataatttt
    20401 aaaagctgga tcatcaagcg tgagtttgtc aatttttaat tccatattta tccccctcgt
    20461 tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga atacatttct
    20521 atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat tttaacatac
    20581 ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag aaaatatatg
    20641 aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac aggacaaaga
    20701 attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg taaattatcc
    20761 ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct tcccaataac
    20821 accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct attaaacgga
    20881 tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt gcttgaaaag
    20941 caattgaaaa aaactattga aaaaaataac tctataattg attctaatct ttctaatgct
    21001 atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt aacaaacgca
    21061 ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac cccaattgct
    21121 tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa tgattcagaa
    21181 aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct tctaaaagag
    21241 ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata aactgcctcc
    21301 tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc aacattcaag
    21361 caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg aatgaacaaa
    21421 atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa agaagcccag
    21481 cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa agcgaacaac
    21541 gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac tgtccgagaa
    21601 gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa atatggaaat
    21661 cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag ctgggctgaa
    21721 actactgacc aatacaaggt acttcattca acttctaagc gtatttttag ctacgctatc
    21781 aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa aagaaccacc
    21841 actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac cttgtttgat
    21901 tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt actacgcttt
    21961 ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc agacattgat
    22021 tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa tggctataaa
    22081 atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga aagaacaatt
    22141 tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact tggtgtaact
    22201 gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca cgctatttat
    22261 gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt gcatgtagca
    22321 agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga agttcaaact
    22381 agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt ggcaaaagaa
    22441 acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta gatggcatac
    22501 aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa ggtttctcct
    22561 agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc ttcaatcatg
    22621 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
    22681 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc aggatataaa
    22741 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat ttcatataat
    22801 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg gtcgtgggaa
    22861 tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa ttgcttacct
    22921 gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat gacttttttt
    22981 cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
    23041 aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt ttctccgtat
    23101 gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg taatccaact
    23161 acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa gccaaactct
    23221 tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc aaaggggtca
    23281 ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct cataaattca
    23341 ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg cgttcgtgac
    23401 ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct ccagaaagca
    23461 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
    23521 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
    23581 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
    23641 acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttccgaat
    23701 aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta tcaaaagtac
    23761 tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca attttttcat
    23821 aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg cttgtcctgt
    23881 ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga attcaatttt
    23941 tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt cattcccctc
    24001 aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat atgccgcaat
    24061 atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg tcatcaagtt
    24121 tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc gattatataa
    24181 ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat acaaaggctc
    24241 tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc ctactctgtt
    24301 caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa gatcctcttt
    24361 tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
    24421 cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa tctgctgaaa
    24481 gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca cagcagcgac
    24541 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagct
    24601 ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata gaaacagcaa
    24661 aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat tcctcgtctc
    24721 taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt tcttcatact
    24781 caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc ttgcatctgc
    24841 ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc tgttcttatt
    24901 acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga atcgaacccg
    24961 cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat acaaaaacag
    25021 tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct ggtatgtttt
    25081 gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt
    25141 agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga
    25201 gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt
    25261 ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg
    25321 aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct
    25381 ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc
    25441 aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat
    25501 cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt
    25561 cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt
    25621 tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt
    25681 tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg
    25741 agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc
    25801 tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt
    25861 ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt
    25921 tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat
    25981 ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg
    26041 cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct
    26101 tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct
    26161 agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga
    26221 agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt
    26281 attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat
    26341 gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa
    26401 agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc
    26461 aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca
    26521 tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa
    26581 aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa
    26641 aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca
    26701 ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga
    26761 ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct
    26821 ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg
    26881 aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata
    26941 aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa
    27001 ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc
    27061 aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa
    27121 gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac
    27181 ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg
    27241 attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat
    27301 tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt
    27361 attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt
    27421 tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct
    27481 tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc
    27541 cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca
    27601 aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta
    27661 aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc
    27721 aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct
    27781 cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa
    27841 gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat
    27901 acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa
    27961 cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc
    28021 atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga
    28081 gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg
    28141 agctgtggat taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac
    28201 atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt
    28261 ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca attcaccttt
    28321 cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa
    28381 aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc
    28441 ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa
    28501 cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt
    28561 ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg
    28621 tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa
    28681 aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc
    28741 cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa
    28801 acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc
    28861 ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac
    28921 attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct
    28981 tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta
    29041 ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga
    29101 gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt
    29161 tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt
    29221 ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac
    29281 ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct
    29341 cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc attagataaa
    29401 ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt
    29461 ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc
    29521 tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc
    29581 gattaataac agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt
    29641 cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga
    29701 tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt
    29761 gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa attctggatt
    29821 cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac
    29881 gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact
    29941 tttcaacata catgttagga ataaattgta attctcttca caatcttttc caacaccttt
    30001 gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat
    30061 acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt
    30121 ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact
    30181 ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct
    30241 gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc
    30301 atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg
    30361 aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg
    30421 atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag
    30481 ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt
    30541 tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc
    30601 tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct
    30661 acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct
    30721 agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc
    30781 tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc
    30841 aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat
    30901 tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc
    30961 aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc
    31021 aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag
    31081 atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa
    31141 cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa
    31201 ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat
    31261 tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg
    31321 acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag
    31381 ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt
    31441 ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag
    31501 ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg
    31561 ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc
    31621 agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac
    31681 gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa
    31741 aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac
    31801 ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat
    31861 cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt
    31921 cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca
    31981 agttgctctg taaattttta cacaactctt attttttacc aaagcacttc aattgacttt
    32041 ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa
    32101 taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg
    32161 ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac
    32221 tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac
    32281 cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag
    32341 gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag
    32401 atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat
    32461 cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat
    32521 cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa
    32581 atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg
    32641 agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg
    32701 attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg
    32761 gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg
    32821 cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg
    32881 gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt
    32941 caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc
    33001 atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc
    33061 tacttgaacg gcttcactta attcagttga agacactttt ttctttccag cattatttaa
    33121 aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg
    33181 atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag
    33241 cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat
    33301 tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa
    33361 ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata
    33421 ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata
    33481 catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa
    33541 tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa
    33601 aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca
    33661 gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc
    33721 gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta
    33781 aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat
    33841 gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc
    33901 gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac
    33961 cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac actttcctgg
    34021 cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat
    34081 tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac
    34141 aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag
    34201 gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt
    34261 gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac
    34321 cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag
    34381 ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc
    34441 ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg
    34501 aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt
    34561 ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat
    34621 ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa
    34681 aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca
    34741 atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac
    34801 aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa
    34861 gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga
    34921 tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc
    34981 tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa
    35041 ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac
    35101 caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa
    35161 gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca
    35221 gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga
    35281 caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat
    35341 gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt
    35401 attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac caattactag
    35461 tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc
    35521 cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg
    35581 tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct
    35641 tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca
    35701 ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac
    35761 aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa
    35821 aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt
    35881 ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt
    35941 ttccatcttt gacatccata taaaatgtat gataagttga accatcttct cttactaaat
    36001 aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga tttccagttt
    36061 cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt
    36121 gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac
    36181 gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta
    36241 ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga
    36301 taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa
    36361 ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg
    36421 tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt
    36481 gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga
    36541 tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg
    36601 gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa
    36661 ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc
    36721 aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac
    36781 tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt
    36841 gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt
    36901 aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt
    36961 ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc
    37021 tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc
    37081 ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc
    37141 aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata
    37201 ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc
    37261 tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc
    37321 aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg
    37381 ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt
    37441 ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca
    37501 atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga
    37561 taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag
    37621 gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa
    37681 ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa
    37741 gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc
    37801 cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct
    37861 aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg
    37921 attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca
    37981 attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt
    38041 ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata
    38101 ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt
    38161 ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg
    38221 ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt
    38281 tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc
    38341 tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc
    38401 ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt
    38461 tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt
    38521 cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt
    38581 aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta
    38641 tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag
    38701 tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca
    38761 gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta
    38821 tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt
    38881 ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg
    38941 taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa
    39001 ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat
    39061 tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca
    39121 tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt
    39181 aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct
    39241 actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct
    39301 agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat
    39361 aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt
    39421 tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat
    39481 aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct
    39541 gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca
    39601 tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc
    39661 atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta
    39721 gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa
    39781 tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag
    39841 cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat
    39901 taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt
    39961 ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac
    40021 agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag
    40081 tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat
    40141 cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa
    40201 taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa
    40261 cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc
    40321 tgtagctaaa actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata
    40381 attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt
    40441 gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata
    40501 tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg
    40561 tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa
    40621 cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa
    40681 aacttgtttt atataaattg actcctacga ccaaagctga attttctggt ccattggaag
    40741 tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg
    40801 tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt
    40861 tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag
    40921 ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata
    40981 ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa
    41041 atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg
    41101 ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg
    41161 ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg
    41221 ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta
    41281 gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa
    41341 acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca
    41401 atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg
    41461 tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca
    41521 gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca
    41581 aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa
    41641 taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag
    41701 tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat
    41761 gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc
    41821 ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa
    41881 cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga
    41941 gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag
    42001 ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag
    42061 aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct
    42121 ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc
    42181 atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt
    42241 ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa
    42301 tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca
    42361 ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga
    42421 tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca
    42481 tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc
    42541 atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct
    42601 acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca
    42661 ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt
    42721 tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt
    42781 aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg
    42841 tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg
    42901 gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat
    42961 ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa
    43021 agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta
    43081 ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac taataaatga
    43141 aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata atccttccct
    43201 tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac
    43261 ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc
    43321 tttttctata gatcaagaaa taacatctat cattactttt ctgaaaaatg aacagttcaa
    43381 aattgattat ctaaaacata gtaagccagc tatttcctat gatcaggaaa tcaattcttt
    43441 tttcatacta tgccaacaga tgagcattca tttgctttcc ttattagtta cttattgtct
    43501 gcaaaaaatt caattgtctt ccttaccaag gcattatcaa caggaacttg ctttattgac
    43561 aataccttca aatgagaaag tcattcctaa aaaaaagcat attctttatt cagatgaatt
    43621 agttgatcca atcatacgat atttagaaga acatctttca gaaaattttt tgcttgaaca
    43681 attagcccaa atttttttta tcagtccagc cagcttaaaa aaacatttta gaaaagtgaa
    43741 tggaaaaagt gttattagct attttaaaga actaaaaatg gaaaaagcaa aacgtctcgt
    43801 gctagaaaat gaattaacct atacagaaat tgcagatata ttaggatttc attcgatcca
    43861 tcatttttct tcag
//
LOCUS       DANJJH010000009        43663 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000009 DANJJH010000000
VERSION     DANJJH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(303..2684)
                     /locus_tag="PWS53_000512"
     CDS             complement(303..2684)
                     /locus_tag="PWS53_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0870782.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2674..3321)
                     /gene="recU"
                     /locus_tag="PWS53_000513"
     CDS             complement(2674..3321)
                     /gene="recU"
                     /locus_tag="PWS53_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL0870783.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3385..3924
                     /locus_tag="PWS53_000514"
     CDS             3385..3924
                     /locus_tag="PWS53_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL0870784.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4014..4433
                     /gene="gpsB"
                     /locus_tag="PWS53_000515"
     CDS             4014..4433
                     /gene="gpsB"
                     /locus_tag="PWS53_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL0870785.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4455..4822
                     /gene="rnpB"
                     /locus_tag="PWS53_000516"
     ncRNA           4455..4822
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWS53_000516"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5031..6197
                     /locus_tag="PWS53_000517"
     CDS             5031..6197
                     /locus_tag="PWS53_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL0870786.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6243..7739
                     /locus_tag="PWS53_000518"
     CDS             6243..7739
                     /locus_tag="PWS53_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL0870787.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7820..8503
                     /locus_tag="PWS53_000519"
     CDS             7820..8503
                     /locus_tag="PWS53_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0870788.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9148..10338
                     /locus_tag="PWS53_000520"
     CDS             9148..10338
                     /locus_tag="PWS53_000520"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL0870789.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10440..11393
                     /locus_tag="PWS53_000521"
     CDS             10440..11393
                     /locus_tag="PWS53_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL0870790.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11462..12811
                     /locus_tag="PWS53_000522"
     CDS             11462..12811
                     /locus_tag="PWS53_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0870791.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12994..13317)
                     /locus_tag="PWS53_000523"
     CDS             complement(12994..13317)
                     /locus_tag="PWS53_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870792.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13446..14609
                     /locus_tag="PWS53_000524"
     CDS             13446..14609
                     /locus_tag="PWS53_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0870793.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14679..16028
                     /locus_tag="PWS53_000525"
     CDS             14679..16028
                     /locus_tag="PWS53_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0870794.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16073..16504)
                     /locus_tag="PWS53_000526"
     CDS             complement(16073..16504)
                     /locus_tag="PWS53_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870795.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16892..19534
                     /gene="alaS"
                     /locus_tag="PWS53_000527"
     CDS             16892..19534
                     /gene="alaS"
                     /locus_tag="PWS53_000527"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL0870796.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19734..20330
                     /locus_tag="PWS53_000528"
     CDS             19734..20330
                     /locus_tag="PWS53_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0870797.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20452..21600
                     /locus_tag="PWS53_000529"
     CDS             20452..21600
                     /locus_tag="PWS53_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0870798.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21638..22051)
                     /locus_tag="PWS53_000530"
     CDS             complement(21638..22051)
                     /locus_tag="PWS53_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL0870799.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22130..22561
                     /locus_tag="PWS53_000531"
     CDS             22130..22561
                     /locus_tag="PWS53_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL0870800.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22660..22893)
                     /locus_tag="PWS53_000532"
     CDS             complement(22660..22893)
                     /locus_tag="PWS53_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL0870801.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23076..24746
                     /locus_tag="PWS53_000533"
     CDS             23076..24746
                     /locus_tag="PWS53_000533"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL0870802.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24893..25279
                     /locus_tag="PWS53_000534"
     CDS             24893..25279
                     /locus_tag="PWS53_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870803.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25266..25964
                     /locus_tag="PWS53_000535"
     CDS             25266..25964
                     /locus_tag="PWS53_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0870804.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25969..26433
                     /locus_tag="PWS53_000536"
     CDS             25969..26433
                     /locus_tag="PWS53_000536"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL0870805.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26430..27341
                     /locus_tag="PWS53_000537"
     CDS             26430..27341
                     /locus_tag="PWS53_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0870806.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27576..28115
                     /gene="pyrR"
                     /locus_tag="PWS53_000538"
     CDS             27576..28115
                     /gene="pyrR"
                     /locus_tag="PWS53_000538"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL0870807.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28129..29412
                     /locus_tag="PWS53_000539"
     CDS             28129..29412
                     /locus_tag="PWS53_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL0870808.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29425..30351
                     /locus_tag="PWS53_000540"
     CDS             29425..30351
                     /locus_tag="PWS53_000540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL0870809.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30370..31650
                     /locus_tag="PWS53_000541"
     CDS             30370..31650
                     /locus_tag="PWS53_000541"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL0870810.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31655..32731
                     /gene="carA"
                     /locus_tag="PWS53_000542"
     CDS             31655..32731
                     /gene="carA"
                     /locus_tag="PWS53_000542"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL0870811.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32736..35921
                     /gene="carB"
                     /locus_tag="PWS53_000543"
     CDS             32736..35921
                     /gene="carB"
                     /locus_tag="PWS53_000543"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL0870812.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35975..36751
                     /locus_tag="PWS53_000544"
     CDS             35975..36751
                     /locus_tag="PWS53_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL0870813.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36748..37680
                     /locus_tag="PWS53_000545"
     CDS             36748..37680
                     /locus_tag="PWS53_000545"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL0870814.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37677..38393
                     /gene="pyrF"
                     /locus_tag="PWS53_000546"
     CDS             37677..38393
                     /gene="pyrF"
                     /locus_tag="PWS53_000546"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL0870815.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38400..39089
                     /locus_tag="PWS53_000547"
     CDS             38400..39089
                     /locus_tag="PWS53_000547"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL0870816.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39062..39331
                     /locus_tag="PWS53_000548"
     CDS             39062..39331
                     /locus_tag="PWS53_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870817.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39369..40010
                     /locus_tag="PWS53_000549"
     CDS             39369..40010
                     /locus_tag="PWS53_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0870818.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40053..40745
                     /locus_tag="PWS53_000550"
     CDS             40053..40745
                     /locus_tag="PWS53_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL0870819.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40742..41644
                     /locus_tag="PWS53_000551"
     CDS             40742..41644
                     /locus_tag="PWS53_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0870820.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41802..43508)
                     /gene="efbA"
                     /locus_tag="PWS53_000552"
     CDS             complement(41802..43508)
                     /gene="efbA"
                     /locus_tag="PWS53_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL0870821.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13986 a         7693 c         9488 g        12496 t
ORIGIN      
        1 aaccatatat tgtatttttt atttgataat aacaatggga ttattaagaa gtggcactaa
       61 ataaaagact agtgtgtatt tagccgattt aattataatc aagtaaatgg ataaagtata
      121 tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag atcacacatg
      181 tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt
      241 gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt
      301 tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc
      361 gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag
      421 ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct
      481 gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt
      541 tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata
      601 gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca
      661 ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa
      721 acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca
      781 ggtgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc
      841 gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct
      901 gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta
      961 ccagttcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga
     1021 gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt
     1081 ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct
     1141 gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag
     1201 atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc
     1261 actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa
     1321 agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc
     1381 gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa
     1441 tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa
     1501 gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc
     1561 tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg
     1621 tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc
     1681 agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct
     1741 tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat
     1801 tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt
     1861 tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc
     1921 tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat
     1981 gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc
     2041 tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct
     2101 tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc
     2161 agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa
     2221 gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga
     2281 atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct
     2341 tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct
     2401 tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta
     2461 tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag
     2521 aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt
     2581 gttccagagg actttccaga acctttgcca gaattcttct tcacactctt tttaggagta
     2641 ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc
     2701 aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat
     2761 accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc
     2821 tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg
     2881 cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt
     2941 catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt
     3001 ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga
     3061 ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt
     3121 cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata
     3181 tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat
     3241 ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc
     3301 atttggatag cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc
     3361 ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat
     3421 cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat
     3481 gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt
     3541 gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat
     3601 ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa
     3661 gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc
     3721 catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat
     3781 acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt
     3841 gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac
     3901 gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat
     3961 acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga
     4021 atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct
     4081 atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat gaagcttaca
     4141 gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat
     4201 tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg
     4261 cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg
     4321 gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact
     4381 acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga
     4441 ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc
     4501 atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt
     4561 actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg
     4621 aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct
     4681 cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa
     4741 aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa
     4801 gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta
     4861 atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc
     4921 gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg
     4981 gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa
     5041 acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag
     5101 aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa
     5161 caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg
     5221 tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc
     5281 cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt
     5341 cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc
     5401 tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc
     5461 agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa
     5521 gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg
     5581 cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat
     5641 gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat
     5701 tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg
     5761 ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt actggttcag
     5821 atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct
     5881 cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg
     5941 tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat
     6001 tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga
     6061 ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt
     6121 acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa
     6181 aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg
     6241 gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt
     6301 taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa
     6361 gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca
     6421 aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg
     6481 tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga
     6541 gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac
     6601 aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga
     6661 taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac
     6721 ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt
     6781 cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga
     6841 gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg
     6901 attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga
     6961 atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag
     7021 tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata
     7081 caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga
     7141 tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg
     7201 cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg aaccatacaa
     7261 aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc
     7321 gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa
     7381 tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca
     7441 tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag
     7501 ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg
     7561 ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat
     7621 atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc
     7681 cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa
     7741 atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact
     7801 taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca
     7861 attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc
     7921 tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta
     7981 cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct
     8041 tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat
     8101 tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg
     8161 taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac
     8221 tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac
     8281 attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa
     8341 actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag
     8401 agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga
     8461 aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa
     8521 gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt
     8581 taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat
     8641 agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt
     8701 ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac
     8761 aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat
     8821 ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa
     8881 ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag
     8941 taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa
     9001 aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca
     9061 aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca
     9121 agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag
     9181 aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt
     9241 gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat
     9301 tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact
     9361 tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa
     9421 ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga
     9481 ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg
     9541 gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc
     9601 cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt
     9661 aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt
     9721 gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat
     9781 gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta
     9841 agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt
     9901 tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta
     9961 ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg
    10021 caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa
    10081 ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg
    10141 ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct
    10201 aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact
    10261 gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa
    10321 gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt
    10381 tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca
    10441 tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc
    10501 tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca
    10561 ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca
    10621 tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg
    10681 cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg
    10741 aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc
    10801 caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg
    10861 gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag
    10921 atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta
    10981 cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt
    11041 tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta
    11101 taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg
    11161 cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg
    11221 aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac
    11281 tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga
    11341 tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga
    11401 accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg
    11461 gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg
    11521 tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt
    11581 attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc
    11641 ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc
    11701 tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc
    11761 agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt
    11821 gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt
    11881 tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc
    11941 tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt
    12001 cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg
    12061 agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata
    12121 cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt
    12181 gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc
    12241 ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga
    12301 cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat
    12361 ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat
    12421 ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa
    12481 agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg
    12541 tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt
    12601 ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt
    12661 tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag
    12721 cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat
    12781 cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc
    12841 acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa
    12901 gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa
    12961 agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat
    13021 tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag
    13081 ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc
    13141 catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat
    13201 aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt
    13261 agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc
    13321 gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt
    13381 tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga
    13441 gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag
    13501 ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag
    13561 taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg
    13621 tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc
    13681 tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac
    13741 ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt
    13801 tattactcat accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa
    13861 cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt
    13921 attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat
    13981 tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa
    14041 gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg
    14101 tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct
    14161 ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag
    14221 taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa
    14281 ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga
    14341 tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc
    14401 agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc
    14461 tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa
    14521 aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc
    14581 aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat
    14641 gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc
    14701 gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg
    14761 tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa
    14821 ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa
    14881 aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca cagatctatg
    14941 aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg
    15001 gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag
    15061 taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg
    15121 catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag
    15181 tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta
    15241 ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa
    15301 tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta
    15361 aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg
    15421 tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct
    15481 tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac
    15541 aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg
    15601 atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta
    15661 tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt
    15721 cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa
    15781 aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag
    15841 aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa
    15901 agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag
    15961 aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac
    16021 ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta
    16081 gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat
    16141 gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct
    16201 gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt
    16261 ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct
    16321 tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt
    16381 gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa
    16441 cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt
    16501 tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt
    16561 accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt
    16621 gatcaataga atgcgtatac taattaagta cgaaaaggat atgatttgtt ttttaagact
    16681 cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt
    16741 ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac
    16801 acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt
    16861 tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg
    16921 acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt
    16981 gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa
    17041 atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat
    17101 tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat
    17161 gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga
    17221 atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta
    17281 tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca
    17341 cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac
    17401 ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa
    17461 ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt
    17521 caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat
    17581 gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt
    17641 gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa
    17701 cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc
    17761 tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct
    17821 tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt
    17881 agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga
    17941 atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg
    18001 tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc
    18061 aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga
    18121 ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc
    18181 acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc
    18241 tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga
    18301 tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga
    18361 agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga
    18421 tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa
    18481 agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca
    18541 aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc
    18601 aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg
    18661 ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc
    18721 agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt
    18781 tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg
    18841 tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg
    18901 cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc
    18961 aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact
    19021 acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt
    19081 gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga
    19141 aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc
    19201 agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca
    19261 gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc
    19321 aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc
    19381 tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg
    19441 tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact
    19501 taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa
    19561 aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca
    19621 tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt
    19681 gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata
    19741 gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac
    19801 aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta acaattatcc
    19861 atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct
    19921 tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata
    19981 cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag
    20041 caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct
    20101 ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat
    20161 tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct
    20221 tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac
    20281 cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt
    20341 ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt
    20401 cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag
    20461 aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg
    20521 ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt
    20581 ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca
    20641 tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct
    20701 tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat
    20761 gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct
    20821 ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc
    20881 aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca
    20941 gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg
    21001 gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat
    21061 agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca
    21121 gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat
    21181 gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta
    21241 gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt
    21301 aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa
    21361 aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt
    21421 aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc
    21481 cctgagaaat tccagtttgt tgatacagaa ctagaaagaa tcttacgtag tcaaatagaa
    21541 gaaaaaatcg gagattacca attggaaatt acgtttgatc acaattatct tttatattaa
    21601 atgagaaact ataaaaagac tccttaaagt ttttaattta aagagtcttt ttttctttta
    21661 attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca
    21721 accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg
    21781 gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt
    21841 ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt
    21901 ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct
    21961 gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt
    22021 ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact
    22081 aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa
    22141 tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat
    22201 tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt
    22261 tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa
    22321 atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg
    22381 acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa
    22441 agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga
    22501 aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta
    22561 attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt
    22621 acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc
    22681 ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat
    22741 gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc
    22801 agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt
    22861 gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga
    22921 tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat
    22981 tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat
    23041 tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac
    23101 aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg
    23161 atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa
    23221 aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg
    23281 aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat
    23341 ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg
    23401 caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg
    23461 acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc
    23521 atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc
    23581 tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc
    23641 caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg
    23701 cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg
    23761 accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga
    23821 aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg
    23881 ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat
    23941 tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga
    24001 aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg
    24061 ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga
    24121 atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac
    24181 aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac
    24241 tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat
    24301 gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata
    24361 gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag
    24421 aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa
    24481 aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa
    24541 cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca
    24601 cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg actggagatg
    24661 ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg
    24721 acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat
    24781 tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat
    24841 tctttttctt tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac
    24901 gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa
    24961 caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc
    25021 ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc
    25081 aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt
    25141 tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt
    25201 tattgtgttg attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga
    25261 aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga
    25321 gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc
    25381 aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa
    25441 ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt
    25501 ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc
    25561 ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa
    25621 agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag
    25681 cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg
    25741 ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt
    25801 atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag
    25861 ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga
    25921 tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt
    25981 tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact
    26041 gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat
    26101 attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata
    26161 gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt
    26221 aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc
    26281 gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg
    26341 atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt
    26401 attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg
    26461 aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa
    26521 ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt
    26581 acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg
    26641 tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa
    26701 acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca
    26761 atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta
    26821 ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg
    26881 cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt
    26941 tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga
    27001 aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta
    27061 ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa
    27121 cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat
    27181 acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt
    27241 ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg
    27301 aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta
    27361 attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa
    27421 gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta
    27481 tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag
    27541 gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc
    27601 aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata
    27661 gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa
    27721 ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca
    27781 ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca
    27841 tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac
    27901 gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat
    27961 tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga
    28021 agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac
    28081 aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa
    28141 aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat
    28201 tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc
    28261 atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg
    28321 tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt
    28381 gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc
    28441 acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg
    28501 ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga
    28561 cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat
    28621 attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt
    28681 tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg
    28741 tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt
    28801 gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca
    28861 cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta
    28921 actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta
    28981 gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt
    29041 ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt
    29101 gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca
    29161 gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt
    29221 ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc
    29281 ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg
    29341 gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc
    29401 gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat
    29461 ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag
    29521 gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc
    29581 tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg
    29641 ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac
    29701 gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat
    29761 gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga
    29821 ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa
    29881 gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc
    29941 gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt
    30001 cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa
    30061 ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga
    30121 aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg
    30181 aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa
    30241 ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga
    30301 gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag
    30361 gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg
    30421 acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca
    30481 gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg
    30541 gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg
    30601 actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct
    30661 gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt
    30721 gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat
    30781 caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa
    30841 acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta
    30901 gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca
    30961 gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat
    31021 ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa
    31081 agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca
    31141 ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg
    31201 aacccgccgc taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt
    31261 acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt
    31321 ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact
    31381 cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa
    31441 ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta
    31501 gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg
    31561 gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac
    31621 ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga
    31681 tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt
    31741 ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca
    31801 aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga
    31861 atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa
    31921 ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc
    31981 aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag
    32041 tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct
    32101 agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg
    32161 tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa
    32221 aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct
    32281 atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc
    32341 aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca
    32401 ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg
    32461 attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca
    32521 tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga
    32581 agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca
    32641 gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat
    32701 ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga
    32761 aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg
    32821 caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt
    32881 ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga
    32941 tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa
    33001 ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag
    33061 aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc
    33121 gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg
    33181 tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc
    33241 gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac
    33301 gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa
    33361 gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa
    33421 tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg
    33481 tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc
    33541 tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc
    33601 cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt
    33661 tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat
    33721 taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg
    33781 cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag
    33841 aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata
    33901 tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac
    33961 ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag
    34021 atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag
    34081 cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg
    34141 aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg
    34201 gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga
    34261 tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat
    34321 tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat
    34381 cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg
    34441 aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa
    34501 tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat
    34561 attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag
    34621 gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg
    34681 ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt
    34741 tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa
    34801 gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa
    34861 gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt
    34921 acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc
    34981 ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga
    35041 tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac
    35101 ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat
    35161 taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt
    35221 atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag
    35281 gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg
    35341 gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt
    35401 cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag
    35461 aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg
    35521 gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag
    35581 aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac
    35641 atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg
    35701 aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg
    35761 tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag
    35821 aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc
    35881 gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga
    35941 caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt
    36001 tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa
    36061 agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt
    36121 acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta
    36181 tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga
    36241 tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt
    36301 atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt
    36361 cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta
    36421 tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg
    36481 tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt
    36541 tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa
    36601 tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt
    36661 ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc
    36721 gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct
    36781 ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa
    36841 tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca
    36901 aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat
    36961 gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta
    37021 gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat
    37081 gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt
    37141 tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt
    37201 caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca
    37261 aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt
    37321 acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta
    37381 gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt
    37441 caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg
    37501 gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat
    37561 ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa
    37621 ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga
    37681 atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag
    37741 actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt
    37801 tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt
    37861 tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa
    37921 agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg
    37981 caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta
    38041 tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt
    38101 ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg
    38161 tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt
    38221 gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca
    38281 caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc
    38341 agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa
    38401 tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc
    38461 caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa
    38521 tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca
    38581 aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg
    38641 ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag
    38701 atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga
    38761 tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag
    38821 aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag
    38881 caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg
    38941 aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga
    39001 aaaaagatcc tgaaaattgg cagattgaag cgcgcttgat gaagatagag aaatccgatt
    39061 tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat
    39121 cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt
    39181 catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat
    39241 atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa
    39301 tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg
    39361 taacttttat ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa
    39421 gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag
    39481 catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga
    39541 aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca
    39601 aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa
    39661 agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc
    39721 gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata
    39781 aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt
    39841 ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg
    39901 ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag
    39961 aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg
    40021 agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag
    40081 acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc
    40141 cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc
    40201 tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt
    40261 tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag
    40321 atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat
    40381 ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc
    40441 taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa
    40501 aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt
    40561 atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc
    40621 atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga
    40681 acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc
    40741 aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga
    40801 agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga
    40861 aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga
    40921 agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac
    40981 tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt
    41041 cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg
    41101 gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat
    41161 cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg
    41221 agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca
    41281 tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg
    41341 ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga
    41401 tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac
    41461 tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt
    41521 aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc
    41581 gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag
    41641 ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg
    41701 tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt
    41761 gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata
    41821 gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca
    41881 taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc
    41941 acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt
    42001 gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca
    42061 tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg
    42121 tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt
    42181 ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct
    42241 tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca
    42301 agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc
    42361 ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga
    42421 tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt
    42481 gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc
    42541 tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt
    42601 tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc
    42661 tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata agctgttaca
    42721 gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc
    42781 gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca
    42841 accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa
    42901 ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc
    42961 tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca
    43021 acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg
    43081 atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc
    43141 attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag
    43201 tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc
    43261 aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt
    43321 tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg
    43381 ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc
    43441 cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca
    43501 aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga
    43561 agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac
    43621 ataaaaaaca gtgtttctat cgttgaaatc caaattttta ggt
//
LOCUS       DANJJH010000010        43355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000010 DANJJH010000000
VERSION     DANJJH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            79..759
                     /locus_tag="PWS53_000553"
     CDS             79..759
                     /locus_tag="PWS53_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0870822.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            849..1883
                     /locus_tag="PWS53_000554"
     CDS             849..1883
                     /locus_tag="PWS53_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0870823.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1897..2232
                     /locus_tag="PWS53_000555"
     CDS             1897..2232
                     /locus_tag="PWS53_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870824.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2216..4696
                     /locus_tag="PWS53_000556"
     CDS             2216..4696
                     /locus_tag="PWS53_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870825.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4752..6311
                     /locus_tag="PWS53_000557"
     CDS             4752..6311
                     /locus_tag="PWS53_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870826.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6308..7672
                     /locus_tag="PWS53_000558"
     CDS             6308..7672
                     /locus_tag="PWS53_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0870827.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7843..8913
                     /locus_tag="PWS53_000559"
     CDS             7843..8913
                     /locus_tag="PWS53_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870828.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            8928..9596
                     /locus_tag="PWS53_000560"
     CDS             8928..9596
                     /locus_tag="PWS53_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870829.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9616..10194
                     /locus_tag="PWS53_000561"
     CDS             9616..10194
                     /locus_tag="PWS53_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0870830.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10597..11508
                     /locus_tag="PWS53_000562"
     CDS             10597..11508
                     /locus_tag="PWS53_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0870831.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11764..12681)
                     /locus_tag="PWS53_000563"
     CDS             complement(11764..12681)
                     /locus_tag="PWS53_000563"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL0870832.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            12883..13320
                     /gene="zapA"
                     /locus_tag="PWS53_000564"
     CDS             12883..13320
                     /gene="zapA"
                     /locus_tag="PWS53_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL0870833.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13378..13925
                     /locus_tag="PWS53_000565"
                     /pseudo
     CDS             13378..13925
                     /locus_tag="PWS53_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            14040..16400
                     /locus_tag="PWS53_000566"
     CDS             14040..16400
                     /locus_tag="PWS53_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0870834.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16487..16801
                     /gene="trxA"
                     /locus_tag="PWS53_000567"
     CDS             16487..16801
                     /gene="trxA"
                     /locus_tag="PWS53_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL0870835.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            16983..18761
                     /gene="uvrC"
                     /locus_tag="PWS53_000568"
     CDS             16983..18761
                     /gene="uvrC"
                     /locus_tag="PWS53_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL0870836.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            18906..19805
                     /locus_tag="PWS53_000569"
     CDS             18906..19805
                     /locus_tag="PWS53_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870837.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            19798..21060
                     /locus_tag="PWS53_000570"
     CDS             19798..21060
                     /locus_tag="PWS53_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870838.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21223..23121
                     /locus_tag="PWS53_000571"
     CDS             21223..23121
                     /locus_tag="PWS53_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL0870839.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(23420..24139)
                     /locus_tag="PWS53_000572"
     CDS             complement(23420..24139)
                     /locus_tag="PWS53_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL0870840.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            24476..24808
                     /locus_tag="PWS53_000573"
     CDS             24476..24808
                     /locus_tag="PWS53_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870841.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            24795..26798
                     /locus_tag="PWS53_000574"
     CDS             24795..26798
                     /locus_tag="PWS53_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL0870842.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            26817..27287
                     /locus_tag="PWS53_000575"
     CDS             26817..27287
                     /locus_tag="PWS53_000575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL0870843.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27308..27892
                     /locus_tag="PWS53_000576"
     CDS             27308..27892
                     /locus_tag="PWS53_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL0870844.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            27907..28893
                     /locus_tag="PWS53_000577"
     CDS             27907..28893
                     /locus_tag="PWS53_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL0870845.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            28883..29194
                     /locus_tag="PWS53_000578"
     CDS             28883..29194
                     /locus_tag="PWS53_000578"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL0870846.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29216..30997
                     /locus_tag="PWS53_000579"
     CDS             29216..30997
                     /locus_tag="PWS53_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL0870847.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            30990..32366
                     /locus_tag="PWS53_000580"
     CDS             30990..32366
                     /locus_tag="PWS53_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL0870848.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32373..33008
                     /locus_tag="PWS53_000581"
     CDS             32373..33008
                     /locus_tag="PWS53_000581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL0870849.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33038..34393
                     /locus_tag="PWS53_000582"
     CDS             33038..34393
                     /locus_tag="PWS53_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL0870850.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34585..35979)
                     /locus_tag="PWS53_000583"
     CDS             complement(34585..35979)
                     /locus_tag="PWS53_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0870851.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36071..37042
                     /locus_tag="PWS53_000584"
     CDS             36071..37042
                     /locus_tag="PWS53_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL0870852.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37039..37821
                     /locus_tag="PWS53_000585"
     CDS             37039..37821
                     /locus_tag="PWS53_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL0870853.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(37990..38361)
                     /locus_tag="PWS53_000586"
     CDS             complement(37990..38361)
                     /locus_tag="PWS53_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0870854.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38495..38938
                     /locus_tag="PWS53_000587"
     CDS             38495..38938
                     /locus_tag="PWS53_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL0870855.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(38981..39790)
                     /gene="yidA"
                     /locus_tag="PWS53_000588"
     CDS             complement(38981..39790)
                     /gene="yidA"
                     /locus_tag="PWS53_000588"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL0870856.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(39809..41176)
                     /locus_tag="PWS53_000589"
     CDS             complement(39809..41176)
                     /locus_tag="PWS53_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0870857.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41160..41990)
                     /locus_tag="PWS53_000590"
     CDS             complement(41160..41990)
                     /locus_tag="PWS53_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL0870858.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42153..42590
                     /locus_tag="PWS53_000591"
     CDS             42153..42590
                     /locus_tag="PWS53_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL0870859.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42666..43286
                     /locus_tag="PWS53_000592"
     CDS             42666..43286
                     /locus_tag="PWS53_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL0870860.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        14199 a         7465 c         9213 g        12478 t
ORIGIN      
        1 aagcggatga attcatgagg attttttgca tactgtctct actatactta ggtcactaag
       61 aaaaaggagt gatttattat gaaaaagaac atgattggga cattattact ttcaacactt
      121 cttctaggcg gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat
      181 attaccttta ctgaaccaac aaataccgtt gagcctctta accctactga tccttcaaaa
      241 ccagtagagc cagcagatcc ggaaaatcca gcaactggcc agacaggttc tttaacattg
      301 gatgtagttc ctgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat
      361 caagtagacg cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta
      421 ggtgcagatg gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac
      481 ggttcacaag ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg
      541 tcagataacg aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt
      601 gcagcgacac caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt
      661 tatgatccag caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct
      721 gatttgactt ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa
      781 actgcggtga aaatactata ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt
      841 ggtggacaat gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat
      901 ttttcatttt tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac
      961 cggtatttcc agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac
     1021 cggaacaaaa tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat
     1081 ttgaggtctc tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa
     1141 aaaacccaac gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga
     1201 aaaaggaaat caatgtatca gcacatgcag aaacaactat acctatagaa gtgaaaatcc
     1261 cagcaaaaag ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag
     1321 ctgaaacaga aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg
     1381 cagtcgtttt acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag
     1441 atttggacga ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta
     1501 ggattatcag caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg
     1561 aaaatacgtc taatgaattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt
     1621 tagctggtga taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg
     1681 acagtaatga atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag
     1741 taaatgaaaa ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat
     1801 tggttgctgc agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa
     1861 agaaaaaagg agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga
     1921 gggattattg caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa
     1981 agttcaggta cgattacatt ttatgatcta agtccagttc cgcatccaag tggttcagtc
     2041 aatggcgcat caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc
     2101 gtattcccgg agacgaatga agaaaagatc cgttttcttt atttgatcat tggtatagaa
     2161 ttatggcttc ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt
     2221 gctacaaaat aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag
     2281 tatatttatc ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta
     2341 tgatccatac acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg
     2401 cgggaagctt gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt
     2461 gaacgatggt tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca
     2521 tgtgaaacta acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt
     2581 ggcgatcatc aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg
     2641 gaacaacgcc tacttgtata ttgccggtgc ccccatcaag gttactagcg gtagtttatt
     2701 atcagcagga agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt
     2761 ggcagatatt attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca
     2821 atttgatgtt acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag
     2881 tccaaacaaa attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt
     2941 ttctgttgat ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt
     3001 agctccagct gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc
     3061 atatgcattt tctgcaagtg cagagataaa tacgtggaat agttacatct caacaagtga
     3121 ttcaacagga aaatataata catttgctga taccacagaa tacgtggatg gaaataacca
     3181 gaaccttcca ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt
     3241 tgatcagttg ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt
     3301 tacacaagga gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc
     3361 caatatcgtg tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa
     3421 ggtattggag cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag
     3481 tcagtgggga aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac
     3541 tgaactaaaa aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc
     3601 gacaggatgg acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac
     3661 agctacttct acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt
     3721 ggacgatgta cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc
     3781 tggaatcacc actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag
     3841 tcagctaaaa aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg
     3901 agatacgatt caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga
     3961 caaaaaaatc acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt
     4021 aaatggtaca actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct
     4081 attgagcaaa ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga
     4141 tacttctgta caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac
     4201 attagcagat ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct
     4261 tgtccccaaa caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga
     4321 tctccccaaa ccgttgaaga caagttatta tgaagcaaaa ggcgattttt cttttgatgt
     4381 gcgggatact cggcttccaa gcacgtctcc ttggcaactg acagggacat tgacttcttt
     4441 atttaagaat gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg
     4501 atcaaaacaa ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc
     4561 taaaggagag gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag
     4621 cagtacgaat gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg aactgacagc
     4681 tgggccaacc agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct
     4741 ctttttcaag gatgagaagg aaacttctgc tgttaaaatc agaaatgctt ggaggagtgg
     4801 acttggaact tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac
     4861 ttttattaga ggaaaaagga gagatttccg ttaataacgt acttacccgc ttatcagttg
     4921 atcgggctac cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac
     4981 aattggaaat tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag
     5041 cagtcgatgt ttattatcat tgctacttgc gctattctac aaaatatcag atacttcttt
     5101 atctgttgga aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt
     5161 cggctacttt gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga
     5221 aaataaaaag cgggcggctg accggtccag aaagtcaaat ttgttatttt tatttccaat
     5281 tattttggtt tggacgtcct tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa
     5341 acactttttt agatctgctg caaaaagagt ttagttttac tattacagaa gaaggacgat
     5401 tgaaatttga aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca
     5461 aaccagatta tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc
     5521 tccatacatt tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa
     5581 cgttggtttt ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg
     5641 tccaggagtt tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa
     5701 cgtttaaaca atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc
     5761 aaaagattgc gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga
     5821 tgaatatttt tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat
     5881 tcctggtttt atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag
     5941 tagacgagga aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttgcggacgc
     6001 tgatcaagca gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa
     6061 tggcggatat cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt
     6121 tagtcgaata cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt
     6181 acccgtcaca gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt
     6241 tcccctattt gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga
     6301 ataaaacatg aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa
     6361 tctaagaaaa cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg
     6421 actaggctat gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc
     6481 tgaagttgcc cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg
     6541 gagagtaact gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa
     6601 aaacgagacc ataacctatg ataaattaat cgtagcaaca ggttcctctc ctgtcacctt
     6661 gtataaacaa attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca
     6721 agtacttgcc gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttatattgg
     6781 attggaatta gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat
     6841 gccgaatgtt ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct
     6901 gcaaactaag caaatcaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga
     6961 agagaaagta gtatccgtac aacttctatc tcaagcaata aaggcagata tggtcatttt
     7021 ttctgcccaa actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga
     7081 tgagactgtg atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg
     7141 agatatcgtc ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac
     7201 tcgagctgta catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta
     7261 cgacctcaga caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg
     7321 gctgacagaa gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt
     7381 tgatctattt ccacaatacg atgaagaaaa caaaacagta aatgcaaagc tgatctacca
     7441 tccggataca ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga
     7501 tttgaacctg cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga
     7561 ggattttgga tttctgtctg aacacacctc tcgttttcac tacctcaatg agttagcttt
     7621 taaagtacta gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc
     7681 acggtctttt attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat
     7741 ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat
     7801 aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat
     7861 gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc
     7921 tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca
     7981 gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg
     8041 atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt
     8101 gcccagacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt
     8161 agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct
     8221 tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc
     8281 gtaggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg
     8341 gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagag
     8401 atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga
     8461 ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat
     8521 gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc
     8581 gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat
     8641 atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg
     8701 gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga
     8761 acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc
     8821 attagcttgt tgatgctggt agtagctgtg ttaggcgcac tattttctgt tcggacaatc
     8881 gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagagcatg aaagcaatag
     8941 aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag
     9001 aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag
     9061 gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat
     9121 ttatcaatga tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa ctacgtttta
     9181 aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc
     9241 agttattatt agtagataag atcaaacatg aacatcgtca aaaagaagca aaagaattat
     9301 ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag
     9361 aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag
     9421 atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa
     9481 aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg
     9541 acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa
     9601 aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa
     9661 gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag agagatcgca
     9721 caaaaagttg ggatcaccca acccaatctc tatcattatt tcggtgataa agaaaaacta
     9781 tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt
     9841 caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag
     9901 acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa
     9961 acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc
    10021 ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat
    10081 tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta
    10141 aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt
    10201 tatataggga gaaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt
    10261 gtttttttta tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca
    10321 gggtatattt ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga
    10381 aattaattgt ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag
    10441 tcaaaaggag atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat
    10501 tttctacggt tatatctttt acagcccctt ttttgcgtac ttaagctgat tttaagtttt
    10561 gacccttagt ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt
    10621 gtatttgcaa tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag
    10681 atagttggac aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt
    10741 gatgatacag aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca
    10801 gatgactcgc taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt
    10861 tctgaaacaa aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc
    10921 ctcgaagatg gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat
    10981 gcagtgattg tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat
    11041 gaacaaacag aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta
    11101 gaggatgctg gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga
    11161 gatatatgcg actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac
    11221 tctacggaat atgcgaatga agcaactgga atcacgacat attattacta tggacaagag
    11281 gaaacattag cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga
    11341 atctccactg ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag
    11401 atgggttata tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag
    11461 cagattgctg caagtttgac acaagcattg accacttatt ttcagtaata aataagccca
    11521 gccaaacaaa aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa
    11581 gcttttgaac accgtttaag gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag
    11641 gagcaatttc aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag
    11701 ttggcatgag aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt
    11761 ccattatttt tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata
    11821 tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt
    11881 cgttccagct cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc
    11941 caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc
    12001 tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt
    12061 aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat
    12121 tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt
    12181 gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat
    12241 ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc
    12301 tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac
    12361 tgttagcgga ccaaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg
    12421 actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc
    12481 ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga
    12541 tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg
    12601 tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc
    12661 tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa
    12721 ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat
    12781 ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt
    12841 acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca
    12901 cgctacaaag cagtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat
    12961 catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca
    13021 ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa
    13081 ttgaagaaac aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa
    13141 atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa
    13201 gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa
    13261 gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa
    13321 gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg
    13381 ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg
    13441 tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat
    13501 ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca
    13561 acatcaaagt tagcattttt tgatcaggta tttgcttttc acctagatga agctttttat
    13621 gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg
    13681 gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga
    13741 ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc
    13801 tttgttccat tagtgtggtc caaaatcaat tcggcgagca tagtttcgct cgttttatcg
    13861 ttgagagaac gccatttctt accaacaaga tgtatgagct ttgggtaacg aatattttga
    13921 attaaggaaa atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa
    13981 ggctgcgtag cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa
    14041 tgaataaccg aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat
    14101 ttgtagtgac tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac
    14161 aaacgatcac aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag
    14221 gcgggatccc tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg
    14281 gagcagactt gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg
    14341 aattgaatcg tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata
    14401 tgtgggcaga tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg
    14461 atgaagacgg acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca
    14521 ttcggcgaag tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg
    14581 caaagtattt aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca
    14641 aacaagaata ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga
    14701 ctttatttat tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa
    14761 tcgctgaacg aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc
    14821 atcgtcaaga aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa
    14881 aagcacgatt tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg
    14941 tcgtcttgaa acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca
    15001 tcacaatcgg aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa
    15061 cgatcacgtt gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc
    15121 ctgcaggtga agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg
    15181 aacagtcgat tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata
    15241 ttttatcaaa agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag
    15301 atccgcaaga aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg
    15361 cttatgtgat ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa
    15421 atacgattaa tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc
    15481 tgattggcgt tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata
    15541 cagaagttat tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa
    15601 tgatcacaga tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc
    15661 attttgtttc tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct
    15721 ttgaagaacg tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag
    15781 aggcggaaga aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg
    15841 ggcaaggaaa tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac
    15901 atcaagaaga aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt
    15961 tgaaaccggg agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta
    16021 aaaacgggaa tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg
    16081 agctgacgcc tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat
    16141 ctgaatctag tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag
    16201 cgctaagtga ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga
    16261 ctattgtcca cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa
    16321 accatcggag cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga
    16381 caattgttaa atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat
    16441 agaaatgcta tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat
    16501 tacagataag acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc
    16561 aacttggtgt ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata
    16621 tgatgaagaa gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca
    16681 acaattcggt atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga
    16741 aaaagctgtt ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata
    16801 aaaacttaca gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt
    16861 ttgcggttga gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc
    16921 tggattgttt ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc
    16981 agatgaacga acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact
    17041 tgatgaaaga taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc
    17101 gtgtccgctc atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa
    17161 tcgaagactt tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca
    17221 atctgataaa gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc
    17281 ctttccttaa gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa
    17341 aagacaaagc actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga
    17401 agatcctcga tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt
    17461 gtctttacta tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag
    17521 tctatacgaa aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg
    17581 agtcagagat ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg
    17641 cagaataccg agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa
    17701 atactgattt actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg
    17761 tccaagtgtt tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt
    17821 tctataacga agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg
    17881 agcatttcat tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag
    17941 ctttgttagc tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat
    18001 tggcgaataa gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac
    18061 aggaacggac gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa
    18121 tacgtatcga gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg
    18181 tcgtattcat tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag
    18241 tcaacggacc ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag
    18301 tgattcgcga agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag
    18361 tagatgtggc aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca
    18421 tggcaaaaaa cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag
    18481 taccgcttga gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc
    18541 atcgattcgc aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat
    18601 tagatgaaat tgaaaggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat
    18661 cgttgaaaaa tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa
    18721 atgtcgcaca aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa
    18781 gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta
    18841 aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg
    18901 aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc
    18961 atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg
    19021 ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt
    19081 tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg
    19141 aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta
    19201 gagggaaatc aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg
    19261 taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc
    19321 agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg
    19381 ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg
    19441 gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt
    19501 tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat
    19561 catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa
    19621 tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag
    19681 atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt
    19741 ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg
    19801 cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc
    19861 ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt
    19921 gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt
    19981 cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa
    20041 aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag
    20101 aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta
    20161 ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt
    20221 tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat
    20281 tttgattcaa aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta
    20341 agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca
    20401 caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact
    20461 gcacaaaagc atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg
    20521 gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc
    20581 aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct
    20641 cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct
    20701 ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta
    20761 ggcggatata tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg
    20821 atcacttcgt atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag
    20881 acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta
    20941 ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga
    21001 ttatggagtt cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa
    21061 aatatagaaa aatatagaag gaagctggac atttattgtt cccagcttct tttttgtata
    21121 gacctatttc tattttttta ttatattgga tatatatatt cattgaaaat agagcataac
    21181 aactttataa tgttgagtat accaattttt ggaggcgttt taatggaccg ctatttagat
    21241 ttactttcag agaagttccc tacaacggaa gatgtcatca cggaaatcat caatctcgaa
    21301 gcaattttgc agcttccaaa aggaacagag ttatttttga gcgatataca tggcgaattt
    21361 cctgcttttg atcatattct gcgtatcggt tcaggaaatc ttaaagaaaa gatcaaagac
    21421 cttttctctg aacaaatgac ggaaacggag atcagccaat tcactatttt tacggcttat
    21481 ccagaatatg tgttgacgac tgattggtac aaaaaacaag ataaatccca gctgatccat
    21541 cgtttgatcg atcttttacg gtttacgaca gtcaaataca ctcgatcaaa agttcgaaaa
    21601 ggattgccga aagaatatag ctatatcatt gaggaactct tgtatatcga tgatcgtatc
    21661 aacgggaaaa aagactatgt aaaaaaaatc attgaacaac ttgtcttgat gaaggaagaa
    21721 gaaaaatttt taacaaaact tgcgcagacg atccaaaaat ctgtcatcga tcatttgcat
    21781 atcgtgggtg atatttttga tcgaggaact caagcagcca aagtaatgga tcgaatcatg
    21841 gatttttcct ctgtagatat ccagtggggg aatcatgata ttttgtggat tggtgcgtat
    21901 tgcggctctg aggcatgttt attgacgctt ttacggattg ccgcacgcta caactattta
    21961 tttgaattag agaaagaata tggattaaat ttacgtccat tattttcttt tgcccatcaa
    22021 acctatcaga agaatccagt atttacccct aaaaacagga aagatctatc tgaaagagag
    22081 caagaagaat tagaaaagat ccatcaagca ctttctatcc tccaattcaa atcagaacat
    22141 cagttgttga atcgacgacc tgaatttaaa atggacgatc gtaaattgtt ggaaaaaatc
    22201 aactatgagg aatcaacgat cgttttagaa ggtcagctct atgagttgaa agggacttgt
    22261 tttcaaacga tccagccaga tgatcctaaa aaattcttgg aagaggaaca aaaagtgatc
    22321 gatagtttga tgtattcatt ccagcactcg ttaagaatgc aaaaacacat gacgtttttg
    22381 atcgaaaaag ggggaatgta tctcacgaac aatcagcatg tactgttcca tggctgtata
    22441 cctgtagatg aaaaggggca gctcttagca tttgaattag accaatctta tcgcggaaaa
    22501 gaactgctgg gattttttga aaaacagata actgaaagcg caaaagattt gactgcaaat
    22561 gaagatttgg cgaccgattt gatttggtat gcgtggagcg ggccattttc gcctttattc
    22621 ggcaagagta aaatggcaac ttttgagcgc tattttctca cagagcctca tacgcacgtc
    22681 gaaaaaagca atccctatta tcacttgcga gatgaagagt ggttcagtga aaaaattttg
    22741 gcagaattcg atgcaaaaga agaaggttca gcaatcatca atggacacac gccagtaaaa
    22801 gtaaaaaaag gagagtcacc gatcaaagca aacggaaaag catttgttat tgacggaggt
    22861 ctatccaaag cttatcaaaa aacaaccggg attgctggct actcattact atgcaattct
    22921 tacggtttcc aaatcgtgac acattatccg ttcttgccta ttgaacaaca atttgcaaaa
    22981 aaatctgatc aaacgaatgt aaaaaaagtg atcgagcagc agctcccacg caaacttaat
    23041 cggaatacga caaaaggaat ggaattacag caacagatta ctcaactcca aaggctgttg
    23101 gattatcgaa aggacgattg agaaaaatgc tagccaacat tgctcaatag actaaaaagc
    23161 gactgcaaca aaaggcgtgt cgcagtctcc ttcggaatta gctcaaaaaa cggtaaaata
    23221 ttagaagcta tttttctgtt tatttctcta atactcaggt cagggcgctg atttcacgac
    23281 ctcttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccaacaa
    23341 acatgagaag ctgttttttg aaattgctcc ttgtgactca gagttgaacg gcaggctcac
    23401 gacctaatca gaaaaacgat taaaattcat tcacaaagaa atcgaataaa cgctgttcag
    23461 atgaatctac acgatgagaa agttccggat gccattggac accaaggatt ttgtgcatat
    23521 tatctttact ttggactgct tcaatgagtc catcgttgga aaaggcgatg gcttttaaca
    23581 aaggagaaag atctttgact gcctgatgat gataggaatt cacaaagtat gaatcaggga
    23641 gaaggtcact taataaactg tccgaaacga ttttgacttc gtgagttgca aactgcgggg
    23701 cagtgggctg ttgttcgtgt tttacggacc attttggata taatgaaaga tcttgataaa
    23761 gtgttccctc caacacgaca ttcaatagtt gaagtcctcg gcagactgca aaaatcggtt
    23821 ttctttgttt caatgcttcc aagatcaatg cctgttcgaa ttggtctcgt tgaatatttg
    23881 tttccgtcag ttttggatga gggtcttcca tgtaaagttg cggactaaca tcctgaccgc
    23941 ctgcaagaat gagtttgtcg attttctcaa ttagttgagg cgctgaatct ggagacatga
    24001 ctggcagaat aagaggtaat ccttgagcat ctaaaactgc atccacaaat ccctgcggtg
    24061 tatagcttac ttgatttcct tggaaagtat ctgtcgctcg aatcagttga ttcccggcaa
    24121 tgccgatgat tggtttcatt tttattcccc ctaaagtaga ctaattgtaa caaggattta
    24181 aaaaaattta aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat
    24241 ctgtaaagag aattttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt
    24301 tataataaaa agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt
    24361 caaggggtga gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg
    24421 tacatacttt caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa
    24481 gcaagtgtta gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg agaagcgtgc
    24541 tcgaacccta cggcagcagt tgaagaccta tgcagaagaa aaagagcaag aattggagat
    24601 aatccaaacg aacagtcggc aacagctgaa acagttggta gaagaaaaag aacaattcga
    24661 aaagcaagaa ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc
    24721 aactttgaaa gaccaatatc aaaagcatca gagtcaagca atcggtgcaa tcattgaaag
    24781 ggtgaaagaa acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa
    24841 gaaatcagga gaggatttta caagcagtac aaggaatcca aaatgttgaa atccgggatc
    24901 tgtatcaaaa tagaacaaac aaccagtggg tagaaaaata ttttccagat accacgatta
    24961 ttgataaaga agcaaaatta agcgctttag gcaatcggct gaaagacatc caagaagcta
    25021 tccaattcat acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag
    25081 aactttcctt acaagacttg gaagcaaatt attccgaaga aaatttcatg aataaattag
    25141 aagaagtcct tgatttaaaa gaaaagtggg aacagctaac tgaaaaacgt gatcgtttag
    25201 aagaagagga agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg
    25261 caagtaagac gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca
    25321 aggaagaact aagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac
    25381 aagtttatct ttcgtatgtt gctttaaaaa aatcagttgc tgctatagaa gaaattgctg
    25441 ggaaatacgg gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaattgc
    25501 aagaaaccaa acagcaatta tccattgttc acgagcagca aaaaaagata acgacagcgt
    25561 taggtcagtg cagcggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg
    25621 ttgcagaacg ggaaaaagta aaagaacggt ttatccatgc cacttatctg atcgttttgc
    25681 aaggttgggt agatgcagaa gaaaaacaga atttgatgca tgtgttaaac gaaactgtat
    25741 cagaagaaga tatatacgtt agtttcgaaa caccaaactc agaagagatt cagcaggaag
    25801 tccctactaa attgaaaaac catccgctta tcgaaccttt tgaattgctg actgaaatgt
    25861 acagtctgcc aaaatatgaa gaagtagatc caacgccttg gatgacacca ttttatcttg
    25921 tcttcttcgg gatgatggtc gcagatgttg gttacggatt gctgatgctc attgggacaa
    25981 tactagctca aaaactattt gttttacccc gcgggatgaa acgtttcgta aaattctttg
    26041 agattctctc ggtaccatcg atcatctggg gattgatcta cagttcattt ttcggacagg
    26101 cattaccaaa agaactattt ggtatccgct tgcctttccc aatcctatct acgacggatg
    26161 acgtcaatac gattttgatt ttatccgtga ttttcggtct gatacagatc ttggttggat
    26221 tattcgtggc agcaaaggaa aatatcagga ggaaagatta cttgagtgct gtcagtgatg
    26281 gttttgcctg gcaagggatc ctgattggga tcgttattgc attgattggt gtcatgttga
    26341 taaaaaatac agcatttgtt tatcttggcg cggggctatc gatcattgct gctttatgta
    26401 tcttaatcgt gccgattatc caatcgccgt ctaaagtcaa gggtgctgcc aaaggagcgt
    26461 ataatcttta cggattgact ggttatatcg gtgatttagt cagttataca cgtttgatgg
    26521 cactaggtat ttcaggcgga agtattggcg cggcattcaa tatgctagtt gctttcatgc
    26581 cgccagccgc tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata
    26641 tgtttttgac actgttaagt gcctatgtcc atggtgcacg attgcagtat gtcgaatttt
    26701 ttggaaaatt ttatacagga ggcggacgtg cctttgaccc gctgaaaacg gcggaaaaat
    26761 acgtcaacat caatcataag aaaaaaaaca ataaataatt ggaggatttt tatacaatga
    26821 tggattactt aattagtcaa aatggtggaa tggtatttgc agtattagcg atggcaacag
    26881 cgacgatttt ttcagggatt ggttcagcta aaggtgttgg aatgacaggt gaggcagcag
    26941 cagcgttaac aacaagtcaa cccgaaaaat tcggacaggc tttgattctg caattgcttc
    27001 caggtacgca agggttgtat ggattcgtta tcgccttttt gatttttatc aacttaggaa
    27061 atgatatgtc agttgtacaa ggattgaact ttctaggcgc ttctctgccg attgctttta
    27121 caggtttgtt ttcaggtatc gcccaaggga aagttgcagc agcaggtatc caaattttag
    27181 cgaaaaaacc tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatatg
    27241 cgattttagg tttcgttatt tccttcctat tagtattgaa tgcctaagca taaaggagga
    27301 aaagcagatg aacgccattg aaaaaatcat ctctcagatg aatgaagcag cagagcaaga
    27361 aagagcggcg ttagagcaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg
    27421 tacgcaaatc gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tacttgaaaa
    27481 aaaataccgt cagcttagga atcgccaaca agtagaagta cgacaagaaa atttgaatgc
    27541 gaagcaggaa tttttacgac gattgtttgc agatgcaata gctgaaatgg agaattggga
    27601 tgaatctgaa caaatccagt ttatcaaaaa ttcattgtat tcattacctt tgacaggcaa
    27661 agtaacattc attgctggag aaaaatcagc agcttatctt tctcagacat tgctggatga
    27721 atggaacaat gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc
    27781 gggttttctg ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca
    27841 agatatccaa ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat
    27901 cagtgtatgg aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg
    27961 tccaatgaga catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata
    28021 ttgaaatcca cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag
    28081 aatcttttaa aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct
    28141 gaaatcgttt ggatctatac gatgcgctat actttccata atctgaaggt tttgacaaaa
    28201 gccgagatga caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta
    28261 gaaaccttga aggatgccat tcatacgcaa gtttcaaatg aactccctga aagcattatg
    28321 gacagtatac gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac
    28381 gtgatttatg atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat
    28441 caagaattgc tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct
    28501 cgaggaatat tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga
    28561 agtatcgaaa aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat
    28621 tttctattag agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatctt
    28681 gatctcgtaa agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca
    28741 caaacccagg ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa
    28801 agcaaaaacc ttcgtttact ggttatcggc aaaagaagcc atttcgaacc agaaacaatc
    28861 agaaagcggg tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc
    28921 tgttttgcca ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt
    28981 tcgtcaggca attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca
    29041 atgtgcagca ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat
    29101 cgtattgatt cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa
    29161 cgtagaaaaa gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca
    29221 aattggaaaa atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc
    29281 cagtatccaa gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat
    29341 gcgtggcgat gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga
    29401 accggttcgc tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca
    29461 aatgtttgac ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt
    29521 cttagggcgc ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc
    29581 aacagtagaa gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac
    29641 aaaagttata aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat
    29701 cgaaaaaggt tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga
    29761 caaaaatttg acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa
    29821 attaaacccg gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt
    29881 gacaaaagga ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca
    29941 gcatcaaatt gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg
    30001 tggaaatgag atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg
    30061 agaatcgttg atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc
    30121 tcgagaagct tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta
    30181 tgacgttgct atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag
    30241 cggacgtttg gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct
    30301 tgcggaatac tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg
    30361 aagtataacc gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac
    30421 acaaaataca ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa
    30481 aagacatttt ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg
    30541 gaagtatatg gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg
    30601 tattttgcag gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct
    30661 ttcagataat gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca
    30721 gcaaaatgct tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt
    30781 gaagatgatc ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt
    30841 aagtgagatc atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat
    30901 cccggaaaca gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca
    30961 aacgatcgtt tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag
    31021 tcgtcggtcc attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg
    31081 aagtccgtat gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata
    31141 aagcaatggt ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac
    31201 gatttttagg ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg
    31261 atggtttggg acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata
    31321 ttaacggaga agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag
    31381 gtatctctgc aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt
    31441 ctggctctgg tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac
    31501 tcgattcatc agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag
    31561 ctgaattttt catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta
    31621 tgaatttagc aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg
    31681 ctgcagaata tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga
    31741 ccaactatgc agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa
    31801 gaggatatcc tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa
    31861 tccgcggact aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata
    31921 aaactcatcc aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta
    31981 gagagcttta caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt
    32041 taaaagataa gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc
    32101 agctttttgc agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg
    32161 aatctgcttt atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag
    32221 aatatgtcaa tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt
    32281 gggaactgct atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg
    32341 acaaatatct gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc
    32401 gcatggaatt gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat
    32461 taaaagataa gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc
    32521 agctgcgtca agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa
    32581 atgcttcgat cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg
    32641 atttgtctat cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat
    32701 atgatgaaca gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac
    32761 tggatcggtc aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa
    32821 tcgaaaaaac ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg
    32881 cattagaata tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc
    32941 tggaagaaaa cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca
    33001 ctgactaagt ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat
    33061 aaagaaacgt ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt
    33121 tggcggcggc ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat
    33181 cgatgcgctt ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac
    33241 agcagagcat tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg
    33301 tcttggtttt atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct
    33361 cagcacacgt attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa
    33421 gctcatgctt tacatcctaa aattggcctt tgttatccaa ttgattggta cggttgcttt
    33481 atcttttgta tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca
    33541 tgcaatctct agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca
    33601 ccagcaaaat gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg
    33661 cttcatcgta tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc
    33721 aaaaatcgca ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt
    33781 cacagagcgc aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac
    33841 cttttttatg agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat
    33901 gagccatgca ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc
    33961 gacggccggc ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat
    34021 gttgaaagga cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt
    34081 gcgggcactt actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt
    34141 atcggtgact gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt
    34201 ctctgcgttt ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg
    34261 gaaattgatc attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt
    34321 ctctctgatg acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt
    34381 catgattgga taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata
    34441 aaataatttt tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg
    34501 cactacccgt tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat
    34561 ttgattcctt tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat
    34621 acttagtcca gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc
    34681 atctggttgc gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag
    34741 aaagtcattc tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta
    34801 agggacacga ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct
    34861 ttttgtaaga cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg
    34921 agtgatgact tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc
    34981 tatcttgctt tggacatgta ctggaactac aagataagca actcgcaatc cagaacaaat
    35041 aaattttgtc attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg
    35101 aatgatcgta tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca
    35161 tagaccttct ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa
    35221 ccctatagga ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca
    35281 ttcctgaatc aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc
    35341 taaatgatat aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat
    35401 tctatcacct ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga
    35461 aacaatagat agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc
    35521 agtagtcaat tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt
    35581 tgggttatta ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga
    35641 actaaccaga ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga
    35701 ctcaaagaca ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt
    35761 ctcaattcca tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc
    35821 tagctctctt tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg
    35881 ttctaattgt tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg
    35941 ccaagttaac gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg
    36001 tataccatac aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag
    36061 ggaattgtct atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga
    36121 agcgattcaa gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa
    36181 tcctcgattg gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc
    36241 tgcttatttg ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa
    36301 tcatcagtat catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg
    36361 actacccaaa gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc
    36421 tgcgctggca acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc
    36481 tggtcaacaa gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt
    36541 gtttgtttac gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca
    36601 gtatcctaaa ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga
    36661 aatcatttgt acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga
    36721 agacgcacat atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc
    36781 gttgatggaa acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg
    36841 ggacctgctt atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt
    36901 aggcgaatta gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt
    36961 cgacgcagtt ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt
    37021 agagaaaggg gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag
    37081 gtgttcaaag gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg
    37141 atgcaaaaaa ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac
    37201 caagaatttg agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg
    37261 acattagcaa cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa
    37321 ttttctactc aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat
    37381 tttcctagta ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg
    37441 gctaagatta aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa
    37501 acggtggcta aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga
    37561 gtcatcgtga cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa
    37621 ttaacaatca atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg
    37681 gcagaaagaa tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat
    37741 ctagaatgtg aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct
    37801 aaaggtacct cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg
    37861 gcaaccttat agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa
    37921 agtttgcgtc tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc
    37981 ccatactaac tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta
    38041 gtaagaaggc gcagtgccag gtaatccttt taagtcagta atctcaagac cggcagcttt
    38101 atgttgctcg tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc
    38161 tcctagatca tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc
    38221 aggaagtgtc aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc
    38281 ttcaaaacca aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac
    38341 acatgtgtga gccattttca tagattcatc tctccttttc cttttactca atcataacaa
    38401 aaagaagtaa ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg
    38461 atagaaaata aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa
    38521 aaagcaattt gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca
    38581 gcagattgca gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga
    38641 aggagaagag acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt
    38701 ggcagaaatt tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc
    38761 aacttattat tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga
    38821 accaactgaa aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt
    38881 gaaacgtggt agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa
    38941 gaaagcgagt agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga
    39001 ttgcttcagc tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga
    39061 caagagaaac ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt
    39121 cgttctcgtt atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga
    39181 gattcgcaac tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg
    39241 cacttttgac cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat
    39301 ctaaaatttc tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt
    39361 cttcaggtgt tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag
    39421 cgctgatatc tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc
    39481 cgacacggcg tgccatcact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg
    39541 cgatgatttt acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat
    39601 cattatcttg aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc
    39661 cagtgcataa aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt
    39721 cttttgttac ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt
    39781 tgatagacat aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga
    39841 tatggctggc aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg
    39901 gatcaaccat ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg
    39961 cagcaacaag ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct
    40021 cgatttgtgt gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc
    40081 tagaattcac agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga
    40141 gttcttggac taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac
    40201 gattcaaaaa acgtttagct aaatcattta aaataggatc agcagattca gaccattgag
    40261 taaagtaagt gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc
    40321 cgtcaagaaa tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat
    40381 acaactcgtg tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag
    40441 gatggaagta cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat
    40501 gcatgccact catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc
    40561 gggtaagatc aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt
    40621 ccattcgatc tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag
    40681 ttttttggat cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct
    40741 gatacacttc tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat
    40801 ggaagatatg ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg
    40861 cacataaagt caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg
    40921 aaaaatttcg agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat
    40981 gagtaaaacg gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa
    41041 tgcgtcgtag acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga
    41101 catggatata gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt
    41161 tataaggggt tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt
    41221 tggttgttcc attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa
    41281 tcacttgcag aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca
    41341 aaaaggggtt ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga
    41401 tagccatttg taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc
    41461 ccgcgttttt cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg
    41521 atccgacgct gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca
    41581 ggacaataag aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta
    41641 gtcaatagga gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg
    41701 gggaaaatca aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa
    41761 tttctgatca ctggagcgta acctgcatct ttcagtgttt ggatcccgtc acttagaaaa
    41821 ggagttcgaa catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa
    41881 aagatttgct ggtgttcttt agaatattcc gttaatatat ctgttaatgc aaaaggggaa
    41941 aagtcgtttt tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct
    42001 cctttgtata aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg
    42061 aaaaaaggtt ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca
    42121 atgtagctat gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt
    42181 caatcaaatt gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt
    42241 tgaaagggca aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg
    42301 aggagacggg cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc
    42361 tgatccgtgc aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa
    42421 catcctatcg aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg
    42481 ttagtttgaa agaccgtccc acaggaggga aggttcgaat cgattatgat ctgttcatgg
    42541 gaccggaaaa aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga
    42601 aaagtttgat tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt
    42661 cctgattgga aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag
    42721 tagcacacgc gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc
    42781 agatccaaaa ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt
    42841 atactgactt gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc
    42901 gttcatggta tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg
    42961 atgaagatac tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg
    43021 aagacgcgat cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg
    43081 acgatgatct gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg
    43141 acttgcaaga aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata
    43201 tcgaagaaga tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag
    43261 aattttctga ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac
    43321 gatcagccta tcagaataag gaggtgtgac acgat
//
LOCUS       DANJJH010000011        41270 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000011 DANJJH010000000
VERSION     DANJJH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            15..692
                     /locus_tag="PWS53_000593"
     CDS             15..692
                     /locus_tag="PWS53_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870861.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            695..1822
                     /locus_tag="PWS53_000594"
     CDS             695..1822
                     /locus_tag="PWS53_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870862.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(2022..2462)
                     /locus_tag="PWS53_000595"
     CDS             complement(2022..2462)
                     /locus_tag="PWS53_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0870863.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(2473..4506)
                     /locus_tag="PWS53_000596"
     CDS             complement(2473..4506)
                     /locus_tag="PWS53_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0870864.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(4508..6211)
                     /locus_tag="PWS53_000597"
     CDS             complement(4508..6211)
                     /locus_tag="PWS53_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0870865.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(6305..7255)
                     /gene="manA"
                     /locus_tag="PWS53_000598"
     CDS             complement(6305..7255)
                     /gene="manA"
                     /locus_tag="PWS53_000598"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL0870866.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(7294..8319)
                     /locus_tag="PWS53_000599"
     CDS             complement(7294..8319)
                     /locus_tag="PWS53_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0870867.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(8528..9397)
                     /locus_tag="PWS53_000600"
     CDS             complement(8528..9397)
                     /locus_tag="PWS53_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL0870868.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(9856..11748)
                     /locus_tag="PWS53_000601"
     CDS             complement(9856..11748)
                     /locus_tag="PWS53_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870869.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(11745..13472)
                     /locus_tag="PWS53_000602"
     CDS             complement(11745..13472)
                     /locus_tag="PWS53_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0870870.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(13488..14138)
                     /locus_tag="PWS53_000603"
     CDS             complement(13488..14138)
                     /locus_tag="PWS53_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0870871.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            14342..14638
                     /locus_tag="PWS53_000604"
     CDS             14342..14638
                     /locus_tag="PWS53_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0870872.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            14810..15430
                     /locus_tag="PWS53_000605"
     CDS             14810..15430
                     /locus_tag="PWS53_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL0870873.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(15522..16688)
                     /locus_tag="PWS53_000606"
     CDS             complement(15522..16688)
                     /locus_tag="PWS53_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0870874.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(16700..17053)
                     /locus_tag="PWS53_000607"
     CDS             complement(16700..17053)
                     /locus_tag="PWS53_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0870875.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            17181..18203
                     /locus_tag="PWS53_000608"
     CDS             17181..18203
                     /locus_tag="PWS53_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL0870876.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            18222..18383
                     /locus_tag="PWS53_000609"
     CDS             18222..18383
                     /locus_tag="PWS53_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870877.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(18642..19076)
                     /locus_tag="PWS53_000610"
     CDS             complement(18642..19076)
                     /locus_tag="PWS53_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870878.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(19186..21045)
                     /locus_tag="PWS53_000611"
     CDS             complement(19186..21045)
                     /locus_tag="PWS53_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0870879.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(21163..22323)
                     /locus_tag="PWS53_000612"
     CDS             complement(21163..22323)
                     /locus_tag="PWS53_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL0870880.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(22630..24054)
                     /gene="araA"
                     /locus_tag="PWS53_000613"
     CDS             complement(22630..24054)
                     /gene="araA"
                     /locus_tag="PWS53_000613"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL0870881.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(24070..24768)
                     /locus_tag="PWS53_000614"
     CDS             complement(24070..24768)
                     /locus_tag="PWS53_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0870882.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(24725..26386)
                     /locus_tag="PWS53_000615"
     CDS             complement(24725..26386)
                     /locus_tag="PWS53_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL0870883.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            26604..28004
                     /locus_tag="PWS53_000616"
     CDS             26604..28004
                     /locus_tag="PWS53_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL0870884.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(28256..29335)
                     /locus_tag="PWS53_000617"
     CDS             complement(28256..29335)
                     /locus_tag="PWS53_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0870885.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(29421..30380)
                     /locus_tag="PWS53_000618"
     CDS             complement(29421..30380)
                     /locus_tag="PWS53_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0870886.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(30392..31903)
                     /locus_tag="PWS53_000619"
     CDS             complement(30392..31903)
                     /locus_tag="PWS53_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0870887.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(31925..32488)
                     /locus_tag="PWS53_000620"
     CDS             complement(31925..32488)
                     /locus_tag="PWS53_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL0870888.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(32494..33357)
                     /locus_tag="PWS53_000621"
     CDS             complement(32494..33357)
                     /locus_tag="PWS53_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0870889.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(33371..34267)
                     /locus_tag="PWS53_000622"
     CDS             complement(33371..34267)
                     /locus_tag="PWS53_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870890.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(34442..35731)
                     /locus_tag="PWS53_000623"
     CDS             complement(34442..35731)
                     /locus_tag="PWS53_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0870891.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(35703..37220)
                     /locus_tag="PWS53_000624"
     CDS             complement(35703..37220)
                     /locus_tag="PWS53_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0870892.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            37418..38308
                     /locus_tag="PWS53_000625"
     CDS             37418..38308
                     /locus_tag="PWS53_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL0870893.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(38381..39205)
                     /gene="nadE"
                     /locus_tag="PWS53_000626"
     CDS             complement(38381..39205)
                     /gene="nadE"
                     /locus_tag="PWS53_000626"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL0870894.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(39217..40689)
                     /locus_tag="PWS53_000627"
     CDS             complement(39217..40689)
                     /locus_tag="PWS53_000627"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL0870895.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        11987 a         8608 c         7141 g        13534 t
ORIGIN      
        1 ttggaggcct atctatgaca ttactgatca aaaacatgaa catgaaacgt ggtaaaaaac
       61 gtgtgattcg acaattgaat cttttagtag aatcaggaga agtagctgct ttgattgccc
      121 cgaacggatt tggcaaaaca actttgctga atggcattgc ccaactgctg ccaacaactt
      181 atgatcaatt gtcccttcac aacatctctc ctactgctta tacagattat cgccggtctt
      241 tcttttttct agaatcaacg aatcagttat ccgactatct atcggctgtt gattatttga
      301 ctttgatcaa atactattgg cagagcaaag tagaatgcca aactgttttc caacgattga
      361 agatgactga gtaccttact ttgccgattg gaaaaatgtc tctaggaatg aaacaacatg
      421 tcttgcttgc tgcttacttg atcagtgatt catctgtcat gttattcgat gaaccgttga
      481 acggattaga cccaggaagt attgatatct tgaatagcct catcagacaa tggcagaaag
      541 aggggaagac gattcttctt tcttcccacg ctatcgcgaa tatccaatcg atttgttcta
      601 aagcatgttt tctaaaaaat ggagaaatcg tcacagaatc ctcaaatatg gaagagattt
      661 tagctattta tcgtaaatta tatgaggtgt aactatgtct ttacttacat ttgaacgcaa
      721 tctcttacta aaaaacaaag taaacttttt atttcctcta ttgttggtcg tgctctttgc
      781 attcccactt ttttttgatc ataaacttgc ttatacagaa ttcgatgaac tgaatcataa
      841 ctatgaagag atgcaacgtt tgattgaaac gttgaaagaa gatgagaatg agaaagaatt
      901 tgtggaaagt ttagagaaaa gcaacaaact gatcgaagca atcttacatg caaaaaacac
      961 agggaatgtc cagcaaatgg tcgaagcaac ttatcattat gaaaaagata tactggatcg
     1021 actgatttct ggtcaaagac aaggaattcc aatcattgaa caacaaaaac gtgtcgagtt
     1081 gctccgttat atgaaagaac atcagatcca aagatattct atttttgact tgccagcgca
     1141 tctcccttta gcaaattact acgaaaatat tttttcagga atgatttcta gttttttgat
     1201 tttatgtata accgccttgt ttctttcttc catcatctct tatgaaaaaa gaaagcaggt
     1261 aatctctcta gtcaatctat taccggatag tatggtaaag aaacatagca tccgttttac
     1321 catttattat ggtgcagcta tgctaagtct cgtgatgcca tttttgatcg tctctatact
     1381 agtaataatc aaaaatggtt tgggagactt ccgttatcct gtaggaacga tcattggtca
     1441 ggagatacga atccttccga tgtatgagta tcttttccag agctttctgt tcctgttgtt
     1501 gtgggtgctc tttttaagta caatcagttt tcttctatct gctttatttg aacattctct
     1561 agtcaatctt ttaggtacac ttctttgtct tttcttagca gagtatcgct tgttttcttc
     1621 gattggatgg atagaatcaa tcagtcatta cttgccaact tcttatgttg attttcaaaa
     1681 tgtgatcata ggaggagata ttttctcgcc actagcttca gaacaggtta ctttcatgaa
     1741 tggtatcttg acacttggta tatggagtat cgttttatta ttcataggta tgggcactat
     1801 ctatataaaa aagagctatt aaaacacaaa aataataggg gcggtgacaa aaaccttgtc
     1861 accgtcccct atttcaaata aacggttttc tagaataaat agttttcaaa agcttgcatc
     1921 cgcagtaata agaacctccc aacaaaaata gctcctctta tttttatctt ggttgaagtt
     1981 attacttgat gcaaggcagc tttttttact gtgaacattg gttatatatg tccatgttca
     2041 aaaatcattt ggacttcttc aatggttttc gcatcactga gcgccattac ttggtcgatg
     2101 tctgaacaga aaaaggctaa atcttggagc accgctaact gatgttcttt ttttaaagcc
     2161 actacaaata agagtgtcgc gatttttggt tcctcttttg ttccaaagtc cacaccatca
     2221 ggcacttgga ctaaatagat tccttcttct actacttctt catctttgtc agtcccatgt
     2281 ggcaaggcta cgaaattccc gatatagaca ctcgcttttt cttgtctttc gatcattgat
     2341 tggatatatg ccggtttgat tcccccttgt tgggtcatcc actggccgat ctcggtcaac
     2401 gcttctttcc aattttgata ggattgatcg agcaatattt tttctgctgg tagctgtatc
     2461 atcttttcac cttcatttct ttatttcttc aatcaggatt ttcgctaaca actgataaac
     2521 gatttcttta tttcctgagc ggaagatctc ggtgttcaag tcattcatga tgattgctcc
     2581 actgatcttt cctaaaagaa tcccgtcttt ttcttggatt ggcgaaggcg ccagcataac
     2641 taacatacgg gacaaatcga ctggctgtct atccatccct tcaatggtca acggatgttt
     2701 caaattgaaa atacaaaaga tcggtttaga aatcaatgag ctggatgtat ggaataaccc
     2761 catatttgta tgaggaatac cgataggtgc ttgttcatat cgcttaagca gccggcgatg
     2821 aaccacctct tcatccgtta ctagtccttc aggaaaataa ggaaataagc tgcgaatcgt
     2881 ttcggaaatc gtctcaggat tatccagttc ttggataaaa aaacgaccga gtaattgatt
     2941 gatttgatcc atgaaatgca ttgtttcctc ataagtatct tctatcatcc actcgttatt
     3001 gctgttatac gtaatttttt gccttttttt cttctcaata tgcgaaaaag cttcttttag
     3061 ctgcttgact tcatcttcta gcagtagcgg ggagactaaa atatattttc caccgtatcc
     3121 tggtaaaata gatgtagaaa taacaacatc atattcttct tgtaaatcga ttttttttaa
     3181 atcagcaatc cgatagaaat gaatttgttg gatgaaggga aaataccttt tcaatcgtag
     3241 ttccaacatg ctagtagaag ctaatccact tggcgaaaat cctgcaatac tgatattgct
     3301 ttctttcgga cttcgttcca atgaattcgc aaaatgaagg accatataag cgatctcttc
     3361 ttcggataat tttttttctt gaaaaatccg ctctactgct ttacggattg cattcgcaac
     3421 ttcctcgtat tgaaccatga ttttttctaa aatcggattg gtcagtgtct cttcttgtaa
     3481 aacggctctt gaaaacacac cagataaatg agtcaacagc attttgtaca aagtttcatc
     3541 ttcataaaaa tggacattcg tctcttcact tacttcttga atcagttgtt ttactaaata
     3601 agcaagctgt gtatcgaacg tctcttcaaa gaaattctga tcaaatgagt tcgtgaagtc
     3661 atttaataat cctgcaaaaa agacgatctc atttactgga taaagttgtt ttgtctcaga
     3721 agcgatccgt gaaaacaatc gtttagctgt ctgtagtaat tctttgtttg ccagactatt
     3781 atatgtctca tttgtcagta aatgattttt agtcacacgc tccatcgtca atgtcagcat
     3841 caaaaccaaa tgttcaagct ttctatcact tagattggtc tttttttgga tcaattcttc
     3901 cccgattact ttgcgtgcaa gagatagatg ttgcggatca acgaatcgaa aaaaataatt
     3961 cagtgatgca tcgaaagaaa tactatgaaa aagttcatat tcattgattt ctaattctaa
     4021 tacattcgct gtcagaagcc ggcggtattt ttccggtcct tcgatctcgt atccctggtt
     4081 ccgaatgatt tccaaaggca gttggcgaat actttcttcc aactgtttga tatctgcata
     4141 aaaagtggta ttactgatgc gatatgattc catgaaatga gcaagactca acggctctgt
     4201 cgtcagtaac aactcggcta aaatcgcgtg ttgtcgttta gcagtggata agtcattact
     4261 tttcccatct gtattttctt ttaattgctc taatacttct tctgatgcct caatgacaaa
     4321 tgagcctcta gctgcttttt tcaaagaagc atttaccgat ttcaatgaga cttcaagatt
     4381 atctaattcg cggtaaatag tccgtttact gaccttcaat aaattcatca ttttttggat
     4441 agatacaggt gacgggctac tgagaaattc tgctaataat tgtttttctc tgggcgataa
     4501 atacatctta tatttctccc ttcacgagtt tttcgtattc attcgtagct acccaatgtt
     4561 caactagaac tagccgaggg atcttttctt ctagctgttc agttacagct agttctcttt
     4621 gtgcgatata aatcgtttct tcttggattt tgattttttc tattgattct tttgagataa
     4681 caaactgttt tttcgctttt tttgctaatt gacttaaaat cgtcatgccc atagtttgtg
     4741 agccgcgtct gttgttttta tacaaaaata caatattagc aggcgacttt ttttgttctg
     4801 tgatatctgc tatctctttt ttttcttccc gctttggaaa ttggatcgct tcttttattc
     4861 gtgacagcaa gatatctgtt tccaaaaagt gatccagtac atagatttga ctttgaggta
     4921 ctttttctct tgcaagaccc gctagttcat gttgcacgat aaccagctga tttacttggt
     4981 tattcaattc atagatagag ctgtatccga cagaaagatc gattttttct gctgctaatt
     5041 tctttttcag cagggcggct cccatagcac ttgatcccat acctgagtcg caagcaaaaa
     5101 tgacggaatg aatgatagat ggttccaaga tttctcccct ttctttgacc gttttgttct
     5161 ctttttcttt atttggtaat ttcaatagta tcgctgcaca aatgaaggtc acaaatgtac
     5221 tgattgctat ccctatactt accccagcta gttgtggata aggcgcattg gcaaaaatca
     5281 cgagtagtga cccaggcgat acaggtgcag ctaatcccgt atctaaccat tgaaatatca
     5341 acgtgccgct tgccccacca gccattacag ctaaaaatag atacggtcgc agcaagacaa
     5401 atggaaaata gatttcgtga atcccgccaa tcgcatggat catcaatgct ccactcgcat
     5461 ttactttttc ctgtttttct gcaaaacaaa ggatagaaag taagatccct aaaccaggtc
     5521 caggatttgc ctccatcaaa aataaggaag agtacccgac tgtttgtgag aattcgattc
     5581 ctaaaggtgt caatacacca tgattgattg cattattgaa aaatagtact tttagaggtt
     5641 ctaagatcag actgacaagt ggtaacagat tcattgtgac tagccaagac acaacggagg
     5701 cgagcaacaa gtgaacacta tttaaaactg gttcaataaa aacgattcca attatacaaa
     5761 taacgctacc agtcagtcct actagtaggt tgctgacaag catttcatat ccagccttga
     5821 atttcacgtg gaatctagaa acaaattgtt catatacgat gccggcaagc ggcccgataa
     5881 tcatcgctcc tacgatttgt ggagcattgg aacgagcgat caaccctaaa gaagcaattg
     5941 caccggcgac tgcccctttt tgtcccccta ccgatttccc gcctgaataa ctgatcaaga
     6001 tgggtaaaag cagctggatc atccaatggc atacctcttc taatgtggtg tgcaaatcgc
     6061 cagacacacc ttttgttgct aatgacaaga ttccccaagc gataaacaga cttagatttg
     6121 gcatgatcat ttcatttaag tgatgtccag cttttctcag ttgtttcata gacacgcgac
     6181 tgctgttgtt tgctttccat ttgtattcca tctttttcac catacttcta tttattgatg
     6241 gacggatttt atgtagaaaa agcagcaaac gaaaaaccgt ctgctgcttt cagctatctg
     6301 ggaactattt gcctggttca gtcgcaatga ttgctagttc accttggacc gcccactcag
     6361 aaacgccatt tggaataata cagcttgttc ctttttctaa tggatatgtt tttccgtcaa
     6421 cgacaaggtc tcctgctcct tcgattaccg tcatcaatgt ataaggggct tgttttttga
     6481 aagatgtgat tcctttaata tcccactcat atacattgaa aaactcagtt tctaaataag
     6541 tgacgatcga tgaattccct tttcggactt ctttgatttg taattctggt gttttcgcag
     6601 ggacagttgt tacatcgatc gattgttgga tatgcagctc tcttgtttgt ccttgcgcat
     6661 cttttctgtc gtagtcatac acgcggtaag tcgtgtcaga actttgctgt gtttctaaaa
     6721 tcatgattcc tttaccaata gcatggatcg ttccgcttgg tacataaaag aaatcacctt
     6781 ttttaactgg tactttcttc aaaaggtcat cccagcgccc atctttgatc atctcagcta
     6841 gttcttctct tgtctttgca tgatgaccat agatgatttc tgcaccaggt tcagcatcga
     6901 tgatatacca gcattcagtt tttcctaatt caccttcatg tttcatccca taggcatcat
     6961 ctggatgtac ttgtacagat agatcatctt ctgcgtctaa gatcttgatc aataatggaa
     7021 acactggttc tgaaggatgg ccaaacaatt ccttgtgttc tgaccatagt tcatctaatc
     7081 ttttcccttt gaattcgcca ttttctacta cactgacacc atgaggatgc gcactgatcg
     7141 cccaatcttc tccgatttta tcgttaggga tatcaaaacc aaagactgaa cgtaaacgac
     7201 ttcctcccca aattttttct tgaaaaactg gttttaaaaa cataggttcc tgcataattc
     7261 aaactcctct catttttcat agctattata gcattatttc ggtaataccg ctttctcttt
     7321 caaaaagttt tcaacaattt catctcgaca tatgagatac tccgcacgat gatcgacagg
     7381 atgtacgcga tttgacaaaa atatcaacgc ttcttgtgtt tttatatcaa tcaagaaaaa
     7441 cgtacctgta taaccagtat gaaaaagaat cgcatgaccg tcttctacat ccttttttaa
     7501 gtcccatcct aaagaacgat agccattttt tttcggtgtt tgatcttgca gtaaagaatc
     7561 aatcgtctct tcttctaaaa aacgaccatt acggattttt cccttgttca aatacatttg
     7621 gacaaaagta atgctgtctt tcaaatcaat aaagagaccc gcgttacctg catgttcagc
     7681 caaaactctt gctttcggat catgagtttg tccacgcaaa acctcacccg aaggcagttg
     7741 ttctgtggga acgatttttt tggaaggaaa aggaagaaat aagctgttca tcattcctaa
     7801 tggttccaat acttgttctt gaaagatttc cactgcagtt tcttggtaaa tttcctctag
     7861 catcaaccca agaagaatcg taccggcatc tgtatatttt acttcttttc ctagttgctc
     7921 acctggttct agcttcaaat aagcggcgat caattcttcc tgagatagct tatctctgtt
     7981 tggtatccat gttttgatat cagaagtatg tgtcagtaaa tgacgcaaag tgattgtctg
     8041 attttcaaat tcaggaagat atttatgaag cggttgatcg agttctatct ttccatcttc
     8101 taatagacgt aaaactaatg aagtggttgc aacgactttc gtcagtgagg ctgcatcaaa
     8161 caacagatct tctgtcatca gctgcttttc aggaacgact tgggcaaagc cggctgtaaa
     8221 tgtttctact tgattttctt ttatgaaggc gactgctaca cctggataaa atccttcttc
     8281 catctttttc aaaataagct gtttcgtgtg tggatacatc tttttctcct gcttttctat
     8341 cagattctta cagtccaagc ttaaagctta atctatgagg aatcaacctt ttttacacaa
     8401 aaaaactgcg cgaaaaaccg cgcagtttcc cccggtatac tattttctct ttctatctct
     8461 agaataagcg atagtatgac cgttcataaa atgacgagac atgcgacaat cgttaagaaa
     8521 gaaaaagtta agcgtcgcat cgaatgtcag ttttattctt ttcttttggc tcactgacga
     8581 accaccattc gattcctata gcatcataga ttgtgagtac tgtgtgcttt ttgtctaaga
     8641 agaaatcaac tgatagttgc tgcaagtgag caatcagtcc ttccatgctt tcagtatcga
     8701 tcatgaattt cagaaaatca agtccaatca cttcatctgt aggcaaatca attgcgcctc
     8761 cgctagcttc tgtcaaacca acatggaaat ctccttcatt caatacatgg atgccctctt
     8821 cttcattatg taccttcaaa ccaagtacat gattaagaaa ttggcgttct tttgttaagt
     8881 tagtaacatt taaatggatt ttttcaaaat aagaacctgt tgatagttta ctgaattttt
     8941 cttcagattt tcttaataat gcttcttgat ccattgtttc tggaatttct ctttggtcgt
     9001 tcttattctc ttcatggtaa atttccaatt cattctcttc tgggtctacg agcaaaatcc
     9061 ccatacgtcc atgatcttct aaagcttctt tgattggata attcatttta cggatacgac
     9121 aaaggatatc tgccatttct tctgtgctag ggataatcaa tgaaaaacgg ttcagctttt
     9181 tgatcacccc catgtgatca ttcgctctag gactttcttc cagcagcaac atttcacttc
     9241 cagcttcttc tgttcctaaa atggctaatt cgttttcttc tcttttcaaa tcaaagccaa
     9301 caacgttctt gtaaaaatca atcattttat ctcggtcttt cacccgaatg gctacacttt
     9361 ttaaataggt ggcagccccc aattgaaaat ctttcatatt tcttgctccc ctctacgtca
     9421 agctttattt taacgatgga ttcttcttct ttcaagcaat aactttgatt ttgttaaaaa
     9481 aaatgtgaga aaattcgcat tttgcttgtt ttttgtaaat taatgagtaa aaactcttat
     9541 aaaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata
     9601 gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
     9661 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa
     9721 aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     9781 ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa
     9841 taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc
     9901 atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg
     9961 attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa
    10021 acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt
    10081 atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg
    10141 cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac
    10201 agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc
    10261 ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact
    10321 tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg
    10381 tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa
    10441 ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac
    10501 catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta
    10561 gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc
    10621 agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc
    10681 aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg
    10741 tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt
    10801 tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg
    10861 catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc
    10921 tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc
    10981 accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa
    11041 aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa
    11101 taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa
    11161 tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc
    11221 catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat
    11281 cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga
    11341 aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac
    11401 gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg
    11461 ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc
    11521 tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa
    11581 tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc
    11641 ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc
    11701 tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc
    11761 tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca
    11821 tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt
    11881 actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat
    11941 gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca
    12001 tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca
    12061 gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca
    12121 aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc
    12181 ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca
    12241 aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt
    12301 tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta
    12361 cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata
    12421 tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat
    12481 gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt
    12541 ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata
    12601 aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca
    12661 atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt
    12721 gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta
    12781 ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca
    12841 aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc
    12901 aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg
    12961 attccgccga tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga
    13021 tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc
    13081 atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg
    13141 atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta
    13201 tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta
    13261 tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc
    13321 caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc
    13381 ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa
    13441 gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta tttatatact
    13501 ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa
    13561 tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc
    13621 actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg
    13681 atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt
    13741 ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac
    13801 aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa
    13861 taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct
    13921 ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca
    13981 aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa
    14041 gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt
    14101 ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggatcctcct ttctttcaag
    14161 atgacacatt gtcatatgac gctgtgtcat tataacacct cctacctgtt ttgcaagaca
    14221 tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc
    14281 catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata
    14341 aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat
    14401 cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc
    14461 tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta
    14521 tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg
    14581 ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat
    14641 attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac
    14701 cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct
    14761 ttttctcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt
    14821 tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac
    14881 gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact
    14941 ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg
    15001 tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa
    15061 tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa
    15121 tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac
    15181 gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat
    15241 ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat
    15301 tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga
    15361 ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa
    15421 atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc
    15481 agcatttgta ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc
    15541 aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc
    15601 actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat
    15661 ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct
    15721 agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt
    15781 actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct
    15841 aaaaatgatc ctaagtaagg gatcaggttc aaaaaacccg caataatccc caacgtgaca
    15901 gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc
    15961 acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc
    16021 aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta
    16081 ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc
    16141 gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc
    16201 atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg
    16261 ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga
    16321 gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc
    16381 ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt
    16441 acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct
    16501 aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa
    16561 acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact
    16621 acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt
    16681 ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca
    16741 gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa
    16801 aaatcaatga tacgtgagct actgtctcta aattcccatt ggccatcgga ccgacataat
    16861 cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt
    16921 cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat
    16981 cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct
    17041 ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc
    17101 attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca
    17161 attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg
    17221 aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt
    17281 cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt
    17341 acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt
    17401 ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac
    17461 gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag
    17521 gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata
    17581 aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg
    17641 tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga
    17701 atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc
    17761 caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt
    17821 ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat
    17881 tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg
    17941 cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca
    18001 ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc
    18061 caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc
    18121 gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg
    18181 tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat
    18241 tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag
    18301 agtatagcga agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat
    18361 atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca
    18421 agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag
    18481 actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat
    18541 aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat attacattta
    18601 tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa
    18661 ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca
    18721 gatgtattga atagatcttc atctacccct gattcaagat cgatactttg gatcatttct
    18781 tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct
    18841 tttgctggtg gacgcaaagt actgactttc ttaagcattg cccgatattt ttttgttcct
    18901 tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct
    18961 tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct
    19021 gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc
    19081 gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga
    19141 caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa
    19201 gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata
    19261 ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt
    19321 ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg
    19381 ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg
    19441 actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag
    19501 aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat
    19561 ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc
    19621 ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa
    19681 gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc
    19741 atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc
    19801 cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc
    19861 tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg
    19921 ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta
    19981 tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat
    20041 cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc
    20101 aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt
    20161 ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa
    20221 tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc
    20281 tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt
    20341 ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc
    20401 accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt
    20461 tttattatat gcttttcgca acaaattatc gggattgttg aaattactga tataccctag
    20521 cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt
    20581 caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat
    20641 cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt
    20701 caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc
    20761 taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc
    20821 aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg
    20881 ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa
    20941 aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa
    21001 aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca
    21061 tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa
    21121 gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt
    21181 ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt
    21241 gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg
    21301 gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc
    21361 taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat
    21421 tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa
    21481 ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc cccctagatg
    21541 aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta
    21601 tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact
    21661 tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat
    21721 caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag
    21781 aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc
    21841 caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag
    21901 accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt
    21961 ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat
    22021 tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt
    22081 tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt
    22141 gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt
    22201 cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata
    22261 ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata
    22321 cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta
    22381 ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa
    22441 taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga
    22501 gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa
    22561 agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac
    22621 aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat
    22681 ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca
    22741 tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa
    22801 attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg
    22861 cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag
    22921 acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt
    22981 cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact
    23041 agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt
    23101 cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc
    23161 tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa
    23221 attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg
    23281 ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa
    23341 ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc
    23401 gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg
    23461 tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc
    23521 cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat cagcaatctc
    23581 tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt tatttaatcg
    23641 agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat tcatgaaatc
    23701 catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat gaagcaatgg
    23761 tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag tatgcatcca
    23821 agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt ttgtgatcgt
    23881 atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc cattttcatt
    23941 cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt cttccccata
    24001 taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca acataattag
    24061 ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc gtaaataatg
    24121 tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa
    24181 attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt gcgcatcttt
    24241 cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta agggatcaat
    24301 tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt acccgctgtc
    24361 gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag tatctgcatg
    24421 cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga
    24481 gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag catgagtagg
    24541 cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt ccacaacgac
    24601 catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa cgaaatatcg
    24661 tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg
    24721 caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt tctatcactc
    24781 ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca cggtaacgtc
    24841 tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg attccttggt
    24901 cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt cgatccatca
    24961 tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg tccatcactg
    25021 taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt gttttaaaaa
    25081 tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat atatccattc
    25141 cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc gctaaatcaa
    25201 aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct gtaatgtttt
    25261 caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt
    25321 tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa gaattggtaa
    25381 attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc gtatgaacca
    25441 tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg acattttcta
    25501 gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg ttccctgtcc
    25561 gtttggctac actgttcgta gcgaccatac cggttcctgc atccccttct ggcggacaaa
    25621 ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct gattcgctta
    25681 acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg
    25741 ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga tggtaggttt
    25801 tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt actttctcac
    25861 cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt tctttcacat
    25921 gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc tgcggaatat
    25981 tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta tttcgccaag
    26041 tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg tgcatcattg
    26101 cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg aatacttctg
    26161 ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat gaataggtcc
    26221 agataccatt ttctagtttg ttttcccact cataactacc agcggcaata ggagtaaaag
    26281 atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct aaagatgtac
    26341 gaccatctat gacatctaac atactttgcg catttacttc cttcataatg atttaactcc
    26401 ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat gattattgta
    26461 tacttttgta cgtacatgtc aagcggttat ttacaagaat acaataaaaa acacttgttt
    26521 tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc gccttctcga
    26581 aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt
    26641 atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt atgaccggcg
    26701 ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg ataggatgga
    26761 tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt cttctttctg
    26821 ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg gctggttctg
    26881 tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca aggattcttc
    26941 taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct gaaatggctc
    27001 ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct tctggcatgc
    27061 tcctttccta tgttgcagat tatttgctaa aagtgttgcc tgaaacgatg gcttggcgcg
    27121 tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt ttagctctac
    27181 cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa agagtattga
    27241 actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag ctgaatgtca
    27301 aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa tatcgttcct
    27361 tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct aatgctattt
    27421 tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc gatgcattga
    27481 tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt ttggttattg
    27541 cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg ggactttcat
    27601 tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg ttgttgattc
    27661 tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca ctgacatggg
    27721 taatcatagg cgaaatattc ccactagctg tccgcgggcg agcatctggc ctagcctctt
    27781 cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg actgcttcta
    27841 tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg gttttattta
    27901 ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa caaagtgcat
    27961 ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt tgtgacagaa
    28021 gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag gaacaatttc
    28081 aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg taggagaaca
    28141 ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca aaagctctca
    28201 caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt
    28261 tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga atatttggct
    28321 gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt cccaactttt
    28381 cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa gaagaatcgt
    28441 ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc aacttatcta
    28501 gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct tgttcttctt
    28561 tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga
    28621 tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa cctttcatcc
    28681 tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga tgtccttttt
    28741 ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa atataagagg
    28801 catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt tctctcaaag
    28861 caacataaag tgctaagttt gggttatact gattactttt cgtaggctca acgatcaaac
    28921 catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg tgatcgttat
    28981 tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg attcctcgga
    29041 taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc gtttttgttc
    29101 tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc gacccttttt
    29161 ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc
    29221 tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt acttcgtatt
    29281 cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt gccatttgcc
    29341 ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt tattcaagca
    29401 aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa ttctggaaat
    29461 ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga
    29521 tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa gcacccgtct
    29581 tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttc agaagaaacg
    29641 aatactggct ccgataattt atgccatgat tcttttaata aaagattctt gtgctcatca
    29701 gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata cgtaatgaat
    29761 attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc ccgtttttcc
    29821 cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca aggattatcc
    29881 atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg ggcccacaca
    29941 taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt ttcccttttc
    30001 gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg ctgattttca
    30061 agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt gcttgcagca
    30121 aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat caattcactc
    30181 atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc taattcattc
    30241 agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa
    30301 taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac gataggatta
    30361 ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta attacattcc
    30421 aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa aaatagttta
    30481 tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga tcgctactgg
    30541 ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc gttatctttt
    30601 gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta tctacgatca
    30661 cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg caaatgattt
    30721 gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca taataagttg
    30781 gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg tttactaaca
    30841 atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac
    30901 caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta ccgacttcaa
    30961 aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac gctagataca
    31021 ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg atttcttcaa
    31081 tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt
    31141 taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc aaagctaaat
    31201 aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg tacgatggta
    31261 aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact aacttcatga
    31321 cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc cccacttgcc
    31381 atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc tttttttgcc
    31441 cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa tattctacta
    31501 aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt
    31561 gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt tctatacctc
    31621 gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta tccacccaat
    31681 cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct aattccttta
    31741 cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg cccggttcat
    31801 aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac agccgagaac
    31861 tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg tccaacagct
    31921 ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat acgccaccag
    31981 caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct aataacaata
    32041 cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa
    32101 tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat aaaagaaaca
    32161 tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc tgttcttcta
    32221 ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac aagttcttac
    32281 caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat ccctcacctt
    32341 tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta ataacaggca
    32401 aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca atcaactgtc
    32461 cataaaattt agtctcaact attttcatct tcctcactct ttcattccag acatagctgt
    32521 tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca gcattgaaat
    32581 aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa ataaagctaa
    32641 ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat aaagcaagtc
    32701 attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt tgctcaatgg
    32761 caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg ctgcctcttc
    32821 caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa atgcagttgt
    32881 catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat tgacttcaat
    32941 aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa
    33001 caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa gagaatcaaa
    33061 gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga tgtaatctaa
    33121 taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat ctgcaaatat
    33181 tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa
    33241 aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa tgattttttt
    33301 accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg gtgccataca
    33361 ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta gtggagatag
    33421 ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag cccaattcat
    33481 aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg
    33541 ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca atcagattcg
    33601 ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta aaaaactgct
    33661 gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag attccctgaa
    33721 tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc atgatggcta
    33781 ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg
    33841 aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa ataatcgcaa
    33901 ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa cgattgaatc
    33961 tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt gttggcgttt
    34021 cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca tcactaaata
    34081 tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg
    34141 taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat aaaactaaaa
    34201 aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat ttggtgaatc
    34261 cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg gtttaaacga
    34321 atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta tctgcaaata
    34381 ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat aaagttctgt
    34441 actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt gtctcaggat
    34501 cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg atcgcagaga
    34561 tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat aattcagata
    34621 caataggatc attttttact tcttcatctt ttgctactga atccaagtag gcaggaaaac
    34681 cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc aaaaactcat
    34741 agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct acgccatcta
    34801 aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa
    34861 tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg gctagttgac
    34921 cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca gtcttgtcta
    34981 catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac gcaaattcag
    35041 acttagtata atcatcgttc actaatttac cgccatttgc taacatccag ttatataacg
    35101 gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta tcagttaatt
    35161 ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta tctaatccag
    35221 cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc atggggacaa
    35281 aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa tcagatttat
    35341 ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt tctacatact
    35401 gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg
    35461 taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt ttgatgtttt
    35521 tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca tcagatgcag
    35581 taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct gctgaacttc
    35641 cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca attaaccatt
    35701 ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac cacattccaa
    35761 gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt tgttgaatga
    35821 cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa tccactcatt
    35881 tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt tagattcatt
    35941 tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat tacggctata
    36001 ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga acgaatttgt
    36061 gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata atagatcgtc
    36121 tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacgtt taccaattga
    36181 gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa tagacagcct
    36241 aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc aatctgccaa
    36301 ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc atcaaaggac
    36361 aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat cgctgtcact
    36421 tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag atcatttttt
    36481 tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc ttgatcaaga
    36541 actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc gcataaaacc
    36601 acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc taaccgtcca
    36661 tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt ccctaaacac
    36721 catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc actccaataa
    36781 gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac tccacgagta
    36841 cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt ataaaattca
    36901 tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct tctaggtcta
    36961 tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt tccaccagga
    37021 tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg gcgaaatcca
    37081 cttttattag ctgatggatg atctggctga taaatccctt catatactgc tcgtcccata
    37141 tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt gacactacta
    37201 atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata atacacctcg
    37261 ttgaaagcgt tgtcaataat ataaatgtta aacttactta ttttttatcg attttaatta
    37321 accaaaatat taataatgat taaaacaaat aatttaatat aacttttatt aacaaatatg
    37381 ttaatatata agaaaaacct tttaagaggt gatatatatg caactacaaa taaacgaaga
    37441 atccttaccg gtatatgaag cattagctag taaaacaagg atcaagatca tccagctact
    37501 ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa cttggagtga gcagtgctat
    37561 cacgacgatg cacgtgaaaa agctagagga agcaaacatc atcaaaacag aaaaagtagg
    37621 acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat attagttttc cagaaaaaat
    37681 attcaatgct tttgatacga aagaaacatc gatacctatt ggtcactata caaattatgc
    37741 aattgaacca acatgcggtt tagctacgat ccatgatttt atcgggaaag tggatgaacc
    37801 acgctatttt atggatcctc gaaggatgga tgcccgcatt ctctggttta ccagcggatt
    37861 tgtcgagtat caggcaccta attttttaaa tacagaggat acattggaga tgttggaagt
    37921 ttctgtggaa atttcttctg aatttccttt ttcaaatgac aattggcctt cagatatcac
    37981 tttcagccta aatggtgtgg agctaggaac atggacgagc cctggagatt ttgctgatat
    38041 ccgcggtaaa tatacgcctg attggtatcc agataatctt aatcaatacg gcttgctgaa
    38101 gacgatccga attaccaaac acttgacgaa tatgaatgga gaacccttgt caaacatcac
    38161 gatcaacgac atccctaaag agcaagacac ttggcatttg cgaatcgaag tgaaagacga
    38221 tgccaaacat gtggggggct gtactttatt tggtaaagga ttcggtaatt acgatcagga
    38281 tatcaaatta aaagtttact acagctgata tttttctttt ctattttata aaacaaaaac
    38341 agccaatccc tatgaaggga cggctgtttt tgctttattg ttatttccag aaatcatcca
    38401 aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt
    38461 cttgtgcttg agcgggaact tcttttcctt ctagataatc atcaatttca ttatatgtga
    38521 cacctagtgc gacttcatct gcaatcattg gtttcccatc ttcaagatct gcagtcggaa
    38581 tttttgtgta taatttttcc ggtgcattca atgcttgaag caattgtttc ccttgtcgtt
    38641 tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc
    38701 cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc tcctgctctt tctccagcta
    38761 cggcatattg cgtaatcata cgttgacgtg ctttgatatt tcctttgtta aaatcgctga
    38821 ttcccacacc tgcttcgctt aatacttgta cttgtgcatc aacagctggt ttgatattta
    38881 ctcgcagact aacatcgggc tggataaagt caagagcagc ttttgcatct tcttcatctg
    38941 cttgttcacc ataaggaaga cgaacagcga taaattgata gttttcatct tttgtttctt
    39001 cacgcatttc ttccatcgtt agttgcgcca aacggcctgc taaagtagaa tcttggccgc
    39061 cgctgatacc tagtacaaaa gttttaagaa acggatattt tttcaaatat gctttcataa
    39121 agtcaatact tttacgtatt tcttcttttg gatcaattgt tggtttgaca cctaattcat
    39181 tgataatttc tgtctgcagg ctcatttatt ctcctcctat gcttctgtat gaagttctgc
    39241 tacgcttttt ttcattcgtt ccatgatagc cattttatgg ttccagcatt cagtagacag
    39301 atcgactggg tatttttgcg gattcaagtc tcgtttatac tcttcccata gtgaatctag
    39361 attttctttt gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc
    39421 ttcgataaag atatcttgta aaactgggcg agctgtaaag tctcgaacag ttttattgat
    39481 aaacgtatgc actggatgga acatgaagat agcttcttct tctcgagggt cttcatccca
    39541 taatgtcacg tagtctcctt cagatttccc atcaaaattt cgagtgatgc gccacacttg
    39601 ttttttccct ggagtagtta ctttttctgc attgcttgat aacttgatcg tatccatcat
    39661 ttttccttca tcatcttcga tcgatactag cttgaatact gcaccaaggg ctggctgatc
    39721 ataggctgtg atcaattttg tcccaacacc ccatacatca attttagctt tttgcatctt
    39781 taaattcaaa atcgttgctt catccaagtc attcgaagca tagatttttg cttccgtaaa
    39841 tccggctgca tctaattgtt cccgaacacg cttagatata taggccatat ctccactgtc
    39901 gatgcgtacg ccttggaagt tgattttgtc acctaattct ttcgccacgc gaatcgcact
    39961 tggcacacct gatttcaacg tatcataagt gtcgactaag aacacgcagt ctttatgggt
    40021 tttagcgtag gccataaatg cttcataatc atttccataa gattggacta acgaatgagc
    40081 atgggtcccg ctagctggta tcccaaatat cttcccagca cgtacattgc ttgttgcatc
    40141 tgctccgccg atatatgccg ctctagttcc ccagactgct gcatccagtt cttgtgcccg
    40201 tctagttcca aattccaata gtggatcttc tccgatgaca gatttgatac gagctgcttt
    40261 tgtcgcgatc aacgtttgga aattgaccat attcaacagt gctgtttcta ctaattggca
    40321 gtgcgctaaa ggcccttcca cttggatcaa tggttcattt gcaaaaacta gttctccttc
    40381 acgagcagaa cgaactgttg ctttaaattc aaaattttgt aaatactcca aaaaaccttc
    40441 aggatagact tctaatccac gcaagtaagc aatatcggaa tcattgaacg tcagattctc
    40501 taaataattg actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat
    40561 ttctcgaaag taacattcaa aaacagcatg taaatcagct cgacctaatt cccagtatgt
    40621 ctgcatcata ttgatttgat atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt
    40681 attattcatc ttgtattact cctcttggca agttttcatt tcctacccat tataacaaaa
    40741 aaaattaata ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt ttacggatat
    40801 ttcttgctaa aaatgaactc catctgctaa aactatcctt tcttattggt atttaaccta
    40861 aacgagaaag aatacttatt ttattcaatg tatccgagac acgaagaaat tagcgttttc
    40921 tatttttctt acttgataaa ataagtagat tttcctacta caatcgtagc cgttaagata
    40981 ccgatgattg aacaaatata accaaatgtc tctacaacat aatcaatggc aaaagttttt
    41041 gaagcaataa tacgtctttc cttttttgct tcttatcccc aaagaaacgg atataactta
    41101 ttatgtattg aatttttttg gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa
    41161 actcgcggaa aaaatatcac acgtttttaa aaagatacaa agtaagaaag cagctgtcaa
    41221 tagaatggaa acaaaaatgg cgagacatca atcagtctcg ccttgttttt
//
LOCUS       DANJJH010000012        41066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000012 DANJJH010000000
VERSION     DANJJH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(352..1134)
                     /locus_tag="PWS53_000628"
     CDS             complement(352..1134)
                     /locus_tag="PWS53_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870896.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1199..2035)
                     /locus_tag="PWS53_000629"
     CDS             complement(1199..2035)
                     /locus_tag="PWS53_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0870897.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2053..3462)
                     /locus_tag="PWS53_000630"
     CDS             complement(2053..3462)
                     /locus_tag="PWS53_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0870898.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3476..5299)
                     /locus_tag="PWS53_000631"
     CDS             complement(3476..5299)
                     /locus_tag="PWS53_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0870899.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6142..6468)
                     /locus_tag="PWS53_000632"
     CDS             complement(6142..6468)
                     /locus_tag="PWS53_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870900.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6514..6876)
                     /locus_tag="PWS53_000633"
     CDS             complement(6514..6876)
                     /locus_tag="PWS53_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL0870901.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6913..7128)
                     /locus_tag="PWS53_000634"
     CDS             complement(6913..7128)
                     /locus_tag="PWS53_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870902.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7255..7590
                     /locus_tag="PWS53_000635"
     CDS             7255..7590
                     /locus_tag="PWS53_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870903.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7666..9663)
                     /locus_tag="PWS53_000636"
     CDS             complement(7666..9663)
                     /locus_tag="PWS53_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870904.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9761..10036)
                     /locus_tag="PWS53_000637"
     CDS             complement(9761..10036)
                     /locus_tag="PWS53_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0870905.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10268..11002
                     /locus_tag="PWS53_000638"
     CDS             10268..11002
                     /locus_tag="PWS53_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0870906.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11041..12183
                     /locus_tag="PWS53_000639"
     CDS             11041..12183
                     /locus_tag="PWS53_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0870907.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12278..13669)
                     /gene="sufB"
                     /locus_tag="PWS53_000640"
     CDS             complement(12278..13669)
                     /gene="sufB"
                     /locus_tag="PWS53_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL0870908.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13674..14144)
                     /locus_tag="PWS53_000641"
     CDS             complement(13674..14144)
                     /locus_tag="PWS53_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL0870909.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14131..15366)
                     /locus_tag="PWS53_000642"
     CDS             complement(14131..15366)
                     /locus_tag="PWS53_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0870910.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15363..16649)
                     /gene="sufD"
                     /locus_tag="PWS53_000643"
     CDS             complement(15363..16649)
                     /gene="sufD"
                     /locus_tag="PWS53_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL0870911.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16662..17435)
                     /gene="sufC"
                     /locus_tag="PWS53_000644"
     CDS             complement(16662..17435)
                     /gene="sufC"
                     /locus_tag="PWS53_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL0870912.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17632..18480)
                     /locus_tag="PWS53_000645"
     CDS             complement(17632..18480)
                     /locus_tag="PWS53_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0870913.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18504..19190)
                     /locus_tag="PWS53_000646"
     CDS             complement(18504..19190)
                     /locus_tag="PWS53_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0870914.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19183..20217)
                     /locus_tag="PWS53_000647"
     CDS             complement(19183..20217)
                     /locus_tag="PWS53_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0870915.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20280..20536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20612..20950)
                     /locus_tag="PWS53_000648"
     CDS             complement(20612..20950)
                     /locus_tag="PWS53_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL0870916.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20952..21308)
                     /locus_tag="PWS53_000649"
     CDS             complement(20952..21308)
                     /locus_tag="PWS53_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL0870917.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21314..22501)
                     /locus_tag="PWS53_000650"
     CDS             complement(21314..22501)
                     /locus_tag="PWS53_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0870918.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22692..23537)
                     /locus_tag="PWS53_000651"
     CDS             complement(22692..23537)
                     /locus_tag="PWS53_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0870919.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23771..24736
                     /locus_tag="PWS53_000652"
     CDS             23771..24736
                     /locus_tag="PWS53_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL0870920.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25113..26156
                     /locus_tag="PWS53_000653"
     CDS             25113..26156
                     /locus_tag="PWS53_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL0870921.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26218..26682)
                     /locus_tag="PWS53_000654"
     CDS             complement(26218..26682)
                     /locus_tag="PWS53_000654"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL0870922.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26928..27875
                     /locus_tag="PWS53_000655"
     CDS             26928..27875
                     /locus_tag="PWS53_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0870923.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28086..30158)
                     /locus_tag="PWS53_000656"
     CDS             complement(28086..30158)
                     /locus_tag="PWS53_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0870924.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30091..30468)
                     /locus_tag="PWS53_000657"
     CDS             complement(30091..30468)
                     /locus_tag="PWS53_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL0870925.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30597..31175)
                     /locus_tag="PWS53_000658"
     CDS             complement(30597..31175)
                     /locus_tag="PWS53_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0870926.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31307..32599
                     /locus_tag="PWS53_000659"
     CDS             31307..32599
                     /locus_tag="PWS53_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0870927.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32659..33777)
                     /locus_tag="PWS53_000660"
     CDS             complement(32659..33777)
                     /locus_tag="PWS53_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL0870928.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33791..35212)
                     /gene="arcD"
                     /locus_tag="PWS53_000661"
     CDS             complement(33791..35212)
                     /gene="arcD"
                     /locus_tag="PWS53_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL0870929.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35458..36579)
                     /gene="mnmA"
                     /locus_tag="PWS53_000662"
     CDS             complement(35458..36579)
                     /gene="mnmA"
                     /locus_tag="PWS53_000662"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL0870930.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36983..37582
                     /locus_tag="PWS53_000663"
     CDS             36983..37582
                     /locus_tag="PWS53_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL0870931.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37720..39006)
                     /gene="efmA"
                     /locus_tag="PWS53_000664"
     CDS             complement(37720..39006)
                     /gene="efmA"
                     /locus_tag="PWS53_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL0870932.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39277..39621)
                     /locus_tag="PWS53_000665"
     CDS             complement(39277..39621)
                     /locus_tag="PWS53_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL0870933.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39758..40903)
                     /locus_tag="PWS53_000666"
     CDS             complement(39758..40903)
                     /locus_tag="PWS53_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0870934.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11805 a         8636 c         7179 g        13446 t
ORIGIN      
        1 gttaacttat tttttatatt tctacatagt tatctttaga accatttcag atacatatga
       61 aacaattaca tatgattttt tcataaaaaa acagtactgt ctagtagtat aaaatataca
      121 aatttcttaa aaattttttc actaaaatca ctacctgaaa cagtctaaaa cacttatata
      181 tcaacgttta atgaaactat ctccgtttca tttcagatac aaataaatat agtcgggctc
      241 caaatccgaa aaaaaatacc cccagtagct tatcctaagc tactggagat gtataagtac
      301 ggtcatataa caacggggta gggtaaataa atgaaaaaga tggaagaatc attattcaat
      361 aactttttta aatacttcgt tgtaatcagt tacctgtccg ttcttaaata ccacagaata
      421 atcttttttc ccattttcaa gtaaccgaag tggaatgtct ttaccaactt gctctgtcac
      481 tgcattactg atacctgtta gattagctaa taaatctgat aaagattgag catctgtaga
      541 aatctctgta tcagtattta tcgtgattcc tttatcttca tttacaagct tccaagtagg
      601 tggtatagct gtgtatgttt cgatgaatga atcaattctt tcatctatcg tcttcccagt
      661 atctacttct gtctcattac tcttagatga atcgattctt tcatccatcg tctgctccgt
      721 aactgcttct gtctcattat tctcaccgga aactctcttc acacccaaac ttatatattg
      781 tccatcttta gtgtgcatag ttaaaattcc cacgctttta ctaataaatt cactataacc
      841 agttctttca atagagactt caatgtattt cacactatca tactcaaaag aaaaatagac
      901 atgtaaaagc attttgttgt ttacatctac ataataatcc tttgactcta cttgagacct
      961 tcctatacgg aatacagctt tactgtctga tatctctata ccagcatcct tattattaat
     1021 ctgccaatct ccaagtagct cttctggcaa gctagtagtt tcttgtgaac actttaccac
     1081 ttcactggta gaggtcgaaa cattcacctc cgcactgtta gaagaatttt ccaaggaatc
     1141 aggagcctta gcacaaccaa caagtcttta tatattgtgc agccgatatt ttcttcattc
     1201 agtacaccct tgaaatatgc agtgttagat aaagcagttc gtcctgattt gctgaggcac
     1261 ccatctcttc ttgaacgtac tccttgattt tcaaggccgc ccgatactct ttcggaaaac
     1321 gctgcttcac tgaagccagc aaaaaatcat ccttctccgg caactcttcc ttcttaatca
     1381 ttcgggcaat aaaaaactgt aaatgcgtca caaatcttaa gtaaagcatc gaatctttat
     1441 caaccttttt ttcgggaaac agatatccta gcatagccaa gatatccttt agtgctctta
     1501 ccgagagcat ggtttcttcc atatttgctt gttcactttg ggcatttaca atgtgaaacg
     1561 caatgtttcc agcctcctct tctggcaact gaacattcat ttttttgttg atcaattgga
     1621 tagcatagca gccgatctca aattcttttg ggtaatactt ttgaatttca atcagcaggc
     1681 gattttgaaa aacaatgcct ttttgactgc gttcaatggc atgcttcaag tgatcaacca
     1741 aggtaaaaaa tagactttta ttcagcttaa agtcaaactt ggcgttggca taatcgataa
     1801 ttcgcttgac gcaaataatt agatcatccg gagcagcctc aaacagttta atgtaatcct
     1861 tttttagagc atcagaaagt gaatctaatt taaatacttt ttggattttg tcagccggaa
     1921 ccaaatcgcc tttctttagt tgaaatccaa tccctttccc catcaaaatt acttctttgc
     1981 cttgcttatc taagcaaaga attaaactgt tgtttaacgt tttaattact ctcattagat
     2041 tcaccgttct ctctaagcta agccatttct tgcgattact ttttgatacc aaaagaagct
     2101 ttcttttttt attcgtttta attctttaag attctcatcg gttcggtcaa catatataaa
     2161 accataacgc ttattgaagc cttgatgggt actaacaaca tcaatcgctg accaaggtaa
     2221 ataaccaaaa acatctacac catctgttaa agctgcttga atagccagaa tatgtttggc
     2281 tagataatca attcgatcct ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc
     2341 cccatagcca ttttcagtaa ttaaaatagg taaatcatag cgatcgtaca atttcctcaa
     2401 tgttaatcgt agtccagtag ggtcaactac ccagccatat ggcgttttat ctacataagg
     2461 attttcagct gcccggtaga caccttcttc tcccaacatg atctgttgat cccccgctct
     2521 agcggaaaca tctgatccgt catttttgct ggcagcgatg gtggcagttg aataataatt
     2581 aatcgcaata aaatcgggtt taccggagga taaaattgcc tgatcctctg cctcaatttt
     2641 aggatataat ccacgatcat tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac
     2701 tgctacatct aagaaagacc agcctctcaa tgtttcccaa ttatgggctg caattgcatc
     2761 ttctggactg gaggtagctt gatacatagc cgttaaattg attgctggac cgatttttcc
     2821 tttaagattc aactgatgat acaagtgaat tactctagct tgagctaata aaacatggtg
     2881 attaatttga aagagttctt tttttgaagg cagctctcca cttggtggca aaccaatcgc
     2941 tccaggatga aggatcatcg tgttttgctc attaattgtc agccaatagt tcaccttgtc
     3001 accatactca gtaagcaata cctgtacata gtccacaaaa taatcgatag ttgctcgatt
     3061 taaccaaccg ccctgctcct ctagaaaata aggtaaatcg aaatggtaca tggtgacaat
     3121 cggttcgatt ccctgattca ggcattcatc aattacttga tgataaaaag aaattccctt
     3181 ttgattggtt ttattatttt caatatccat aattcgcgtc caagcgatgg aaaagcgaaa
     3241 agcttttaag cctaattctt taaacagctg tatatcttct ttatagtgat ggtaaaaatc
     3301 cgaggcaact tcaaaattag ctaccccttc tgtatgctga tacttgtcaa taatagacgg
     3361 ttgtttccca tcctcctgat aggcaccctc tgcctggtag gcactaatgg aggcacccca
     3421 taaaaacttc tctggaaatg ctgaaagctg tttgtattgc acaaaaatcc tctccttaac
     3481 taatttgcat caatggttcg tgttccttta agcttccgct tgcttcacta attcctgcgt
     3541 aattttcact gttggtcact accacaatca ctgtatcatc gtagccagcg tttctaatgg
     3601 cagcgaaatc ggcgttgatt aataattgtc ctgctgacac ctgatctcct gcttttacaa
     3661 atacttcaaa gggttcccct tttaaattca cggtgtcgat ccccacatgg atcaataatt
     3721 caataccttc tttagaacgt agaccaatcg catgtttaga ttctaaaata gtaatgactt
     3781 ctccctcaac aggtgaataa atgctgtttt cttctggatg aatggctacg ccctttccca
     3841 atagtccctt agaaaagaca gcatcgttca cttcagaaat aggacacacg gttcctttta
     3901 ccggattttc aacaatgact gcttcggcta ttgccgtttg ctctgattca tcagaagtat
     3961 cgtcaaatcc aattagataa gcaatgataa aggttgcaac aaaggcgatt acacacgtaa
     4021 taattgcgtg aacaatattc attggatttt ctccaataaa ggctggtaag gctgctaagc
     4081 caggggaaac aaaggcatat ctgactaaac cgctcaagcc agcatacaaa ccagcaattg
     4141 caccaccaat catggaagca actaatggtt ttttcagctt caaggttact ccatagagtg
     4201 aaggctcagt aattcccatt aaagcagtga tcccagtgga cacagctagc tgcttcaact
     4261 ctttgttttt agttttcgcg gctacagcaa aggtagcacc tgattgagca atattagaag
     4321 ccagcattcc tggcccattg atcatttcag aacccgtact gctcaattgc atagtagcga
     4381 ttggcgtcat tgcccaagct gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca
     4441 aaaatggaat taaccaagct gctttgccgt taatccattc agcccccatt gctataagat
     4501 cgttcagcaa cgttccaaac ggtccaatga caaccaacgc taaaggcccc ataattaaaa
     4561 ataccagcat cggcttagta aagaatttaa taaacgaagg cgaaattcga tccgcaaagg
     4621 gctctacata ggacatgatc caaacagtaa agataatcgg taaaactgat cccgcataat
     4681 ctgcagctaa taccggaatt cctagaaaag aaacggcatc gccagtagaa aaaagctgtg
     4741 aaatatttgg atgaagcaaa atgccagcaa cagtaatcgc caaaattggg ttgacatcaa
     4801 actttatggc tgccccatag gctaatagta ctggcataaa ataaaaggct gcatcagcaa
     4861 tagtattcaa aatgatgtaa gtttgactac tgtctgatag tacaccagtc aaagtcaaga
     4921 cggccaaaat ggcttttacc atcccggcac ctgttatagc tggaatcatc ggtgtaaagg
     4981 tggtagaaat gacactaatc aatcttgaaa taatactttc ttgattgtct gaagaggggc
     5041 cctttttaat tgaagttccc acttcctttt gaatttcctt gaaggctgtt tggacttcat
     5101 tgccaatcac cacttggtat tgtccaccgc tatccaccac actaattaca ccaagtgttt
     5161 tttctaaggc tttcgtatcg gctttccttt tatctttgag ttcaaaacgc agtctggtgg
     5221 cacaatgggt taagctgacc acattttctt ttcccccaac ctgttgcaat acatttcttc
     5281 ctacttctct gtaatccatt attctctctc cttaaagcag taaaaaagac caagtcaaac
     5341 cacgccccaa tcaacattga agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac
     5401 atgccaataa cgttattttg ctgtaatcca ataatagcac attatgatag cgcaatcaac
     5461 agggaattta attttttatc ttttcgtttg accgtatagg gtaatataac agttagatca
     5521 catcaactag ggtatatctg cgtatactta ctgctaaaat aagtatatct aatattaact
     5581 ataccagtaa aaccattcct acataaccag cgggaaattc ccaaaatcgt gaaagcatca
     5641 aaaatgcata taacggatca cacaacttca acaattctgc tcttgggaaa aattgatgaa
     5701 tttggtgagt aaaggatgga ataaacagca ttccaacaat ataggcaaca atagtgatac
     5761 ttgttttttt ggtaaaaatc agtaagcaat agccaatttc aatcattccg agatataaag
     5821 gtatcgatag aatgatttgg gctagtattc ttaaaaatta actagcaaat gtttaaactg
     5881 atttatttct aatctacatg tagtaagtat ttcaattcca ccaattatca ataaaaatac
     5941 caaacatatc cataataatt ttttcttttt catttctttt atattgttat aacagtgaat
     6001 aacataaaaa ttcccgacaa tcattgtaat atttttttgt cacctgtatt agtatttaat
     6061 ttataggaaa ctataaaaac tccctagtga atttacacct ttgggagttc ctgtttggac
     6121 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagcatatg gtatctccac
     6181 atatatacgg tctttctttg gatagtactc aatatttatg ttgatagcac ttccattgtc
     6241 taatatagct ttagcccggt atgtgttatc tccattttca tgagtaatcg tcacatcatc
     6301 acatgatcga tttaagttgt actgttcttg taaaatctgt gttaccaatg gttttgatga
     6361 cttctctaat ccagatgtac ctgtcatttt tgaaaaaata atacctataa tagccaagac
     6421 aacaatacct ataatgacaa acttcttgtt catatttact ttattcaact ttttatctcc
     6481 tatctccaaa ataatttgct cttatccctc ttatcagtac attgacatcc ctaaaagtac
     6541 accaaagatt gctccagcta cccctgctat catcaaaatc gtacccattt tctcatcttt
     6601 ttctcgatat aagtagccca taataacagc aacagcacca aatacaacag gaataaatgc
     6661 taacgatact accatagaaa tccaaccaat aatcaaatac agcttccctg tctcttgtcc
     6721 atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct ttactgtgtc
     6781 tgttccggtt gtctcatctg tttcttgtag tgctccacaa aactggcaat acttactttc
     6841 atctgttatt tctttgccac attttctaca atacatgtct tctcctccta aaatcattca
     6901 attgcttatt agttaactgt ttactttatt aaatacatca gaaaacagct cattcattgg
     6961 tacacttaat atctcagaaa gcatcttcat ttgatagggt ttaggaatcg ttctccctgt
     7021 ttgccacgaa cttacacatt gctgtgtaac attcattttt tttgctaact ccgtttgaga
     7081 aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga ttctcccctc
     7141 ctgttttatt atatacaaca atatgatgta tttcaactca taaaatataa aaaacttcaa
     7201 ataccattac aaatacaaca acttgttgta ctatactgag atgggggtgt attaatgaaa
     7261 cagaaacaat atgcaaaggt cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa
     7321 gaattagctg aaaaaatctt ctctacacaa caggcagtag caagatggga aaattctgta
     7381 acagagccta acctcgatag cttaacagct ctttctagag ccctaggaac ccctgtaagc
     7441 cattttctag acaatgttgt cgtagattat gaggaagagt ttttggctct ctatcgttct
     7501 cttagcactg aagatgcagt tcgaacaatt gactatatga agctattaaa aaggcaagaa
     7561 aatgaacgca atcagctatt gaaagattga aaaataaata acccaccgaa ctttaacggt
     7621 gggtgtattg agatgaaata agattaagag ctatggataa cctgtctagt ttaatacaat
     7681 tgggtctttt ctcttcctta ctctccgtcc atcatctctt gttacacttg taaattgata
     7741 cagaggaact atcccagctc ctgataatat caattctttt attgtttcat ctatccctgc
     7801 ccctttcgct aaatatatta aaaaactact acttgctttc acattgtata gtttcttaat
     7861 atccttagaa gctcgttcaa gagtgaatcc ttgtttaaca aagtagcctc tgttttttat
     7921 taaagcattt ttcacgctgt tatccccaac attacgtagg cgttcacaaa cagtgttaat
     7981 aagttctttc tctgtcatta gatatgtccc ttgccacgat aagccctgta aaacttcatg
     8041 tgcttcatct aaaatcaatt gtttttcctt gctgagttct gatcgggaat tttgaggaaa
     8101 tactctttta gcctcttttt cgttaaaaca tatcttaacc cattcataag aaaaatattg
     8161 atatgtaaaa tgaatttttt ccagaagtgg aaggatatct tcattcaatt tttgaatcac
     8221 agaagttgca ttaaaaacgt ctaatcgata gacattgctt ctcttatgat taaagtttcc
     8281 taacgctttc tcttccttta acctatttag gtattcgagc atcttcaaag gaacatcttc
     8341 atcatctaat ttttttatca atcctataaa agtcataagt gatagtaact tattacattt
     8401 atctatgtca taagttttac catagtaaga actcattttt ttagctaatt gttcccccga
     8461 caaaatacaa gtcacacttt ccttactatc atcaaaaaaa ttaacatcag aaagcaagac
     8521 atcatagaca cgtcgcattt cctctagata attccttatt gtcctgaaaa gtttgtcttt
     8581 cattgattca agagtgtcaa aaagtacttt tgattcctct ttcaattttc gaaaatgtgt
     8641 aggatacaag tctaggttct ctaatagcca attaaatgtt tctacccgac tgtctttctt
     8701 taaaattagc atcacaactc caataaaatt ttcactgata tcacaattac tggaatgaca
     8761 ataaaaaatg gaatgcccag ttttactggt aaaaatacta gcagagggat tatgatcgtt
     8821 gtgaaacaga catcttaagt ttttacttga cttaagtaat ttattcatat caatggttaa
     8881 caatctttca taaatctcat taaaagacat gtcggaggaa tcaaacaggc agtctttaaa
     8941 ccactgtttc atttcttctt tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt
     9001 gactagttca tactctctcc ctgaatcact ttgacaattt ttagaacatg tcatggtaaa
     9061 atcaatcgga agctcagtaa tatctaacat gttgtcttca ttaattatta tcactgcatc
     9121 tgacttatat cctccaaaaa acattctatt cgaagcattt acattgtcat cacacccggg
     9181 gatgttcttt ttcaagtatt cgtaaacagc aaccatttta ccatagtcag taattgcttc
     9241 ttttaataga aagacaatct tgtatttttc tagattttca gagtaccgaa tacttttttg
     9301 tatcgcaaac gcagaattat gaactaaatt gatagataaa accttttcta cgcttaagta
     9361 tctactatca tctttactca aatctactac cttccccttc tcatctttaa cagtgttatc
     9421 gacatccaga ataattactc tggtttccac aaaactctcc ttgtcattgc ctttattctt
     9481 tgtcttgctt ggaataaatg attgtgatga atttagtatc tctacaaact catctcttgt
     9541 aacttctcta tagttatcta aacacctatc tcttacatca gttttttctt tttttgtaac
     9601 tggcttctct aataattttt cttgatctac ataaattgga atattaatca ttgatgtttc
     9661 catttgtgtt tcctccttat aaaattctgt taaagactaa aacatacaga taataaaaac
     9721 aaactcttta ttactatatg ttttagtctt atttgtttac tcaaccttta taccgttcca
     9781 tgaaactatc aatatcagat tttttaaatc taatgaccgt atcaattttt ataacaggta
     9841 acccgtattg agaaacatat tttctaatcg ttcctactga tacacctaga tattcacttg
     9901 cctgctgata gtccatatac tctagagtga ctccagaaat aatgtcttct agcataggct
     9961 ttagcactgt aattatctct ttcacttgtt gttcaacttg tatagaaaca atgctatcaa
    10021 tgatttgtag agacatattt ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa
    10081 ttatcttcgt attttttgct caaaataaaa gtattttgtt attaaccgat taatcaaaca
    10141 aaaaatgatt accagttcat cgacatacac ataatacctc gataaaaaag gggtgtcaac
    10201 agctttgtaa aatttatttg caacacccct tttttgaggt atactaattt taggaggtgt
    10261 ggtcaatatg ataaaacgtc caccaataaa ttatttagaa cgaaaaaaga tactcggaac
    10321 aaaaataaaa gctataagaa agagtaaaaa attaacacaa ccagcatttg gtcttatgat
    10381 taacaacggt caattaattg acaaaaaaac tatctacgaa tgggagaagg gaacctattt
    10441 accaatacca gagcgattat cccgaattgc tgatttggga aatatgagta tagaggagtt
    10501 agtttgtggc aatgtagaag aatatatttt gggaattatt ctttatagag atagcattgt
    10561 actagatggt ataacatttc cagataaaaa cctgttccag catcttcgtc aacagttccc
    10621 accagtacac tctaatttag atacatggct ggatagatat tccaaattag aaccagagat
    10681 gcaagaattt attgctaaca agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt
    10741 caatatctta aaaatagaag aattattcat caatgctatt gtagaagaat ttgacaataa
    10801 cattcttttc ctcactagca gtatagaaga actactagaa agaatggtag atgaatggct
    10861 acctattcaa ttgaaagaca tgtcttatcc cgaagaagct gtgagagaaa ttactgataa
    10921 tataaacaag ctagaacaaa cgatttcatc aattgggaaa aaatacacta agaaaaaaat
    10981 gaagggaggt gataccatct agtggtaagt actccttgta aactaagagg agatgattta
    11041 atggcaaaaa tatcaaagta caaaaagaaa aacggtcaag tggcgtggat gtataaagga
    11101 cacattgcca ccgatccacg aactggtgaa aaaataaata ctacgagaag aggttttagt
    11161 agcaaacaag aagctcaaaa agattatgac gagtacagac atcaaatatt atacggtgtt
    11221 aaaaagaaag cacctgatat gacatttgaa gacctgtata atgaatggat aactcatcag
    11281 cgtaccagtg ttaaagcttc aacaatcgca atctcagtaa gatacgcaaa taatcaaata
    11341 cttccagcat ttggtaagct taaactatcg aatatctctg tcccatattg tcaaaaagtg
    11401 gtagatgaat ggcacagtaa atatgaaagt tacgattata tgagaaaaca gactgcacaa
    11461 attttacgct acggggttgc aatgcaatat attgacaata atcctatgga aaaaacactt
    11521 cttccacgaa aaaaagagta tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat
    11581 aatttactgg atgcttttaa agattttggt aacatgaaac aatacgcctt cttcagatta
    11641 ttggcataca caggtatgag aaagagtgaa gtactcgctt tacagtggaa agacatagac
    11701 acgtttaata aagaactaca tgtcaataaa actcttgctg tagatgaatt tggtaaagtt
    11761 ataattcaat ctccgaaaac aagagcttct agacgagtta tctctttaga tactgagact
    11821 ctttctatcc tcaacaactg gaaattacag caaaaagaag agtatttaaa acttggatac
    11881 aatactagct ctaaagagca acatgttttt acaacagtta aaaacactct atacattcca
    11941 aacactgtta atgattggtt aagatatatt ttgaaaaaat ataacttgcc tcgtattaca
    12001 cctcatgggt ttagacatac acatgctagt ttattattag aagcaggaga atctgtcaag
    12061 gtagttcaac aacgattagg gcatgaaaat agtaaagtga cattagacat ctatgcacat
    12121 attacaaata atgcacctaa gaaaacaggt caagactttg ctgatatgat ggctcatcag
    12181 taaaagagct ggtcaaaatc tggtcatttc tagtatacag gcaaaacaaa aaagctagaa
    12241 cccttatata tcaagggttc tagctcctat aacaggttta cccaacactg ccttccatct
    12301 cataactgat caatcgattg agttcgactg catattccat tggaagttct ttggtgaatg
    12361 gttcaacaaa gcccatgacg atcatttcag ttgcttcaga ttcggtcagg cctcggctca
    12421 tcagatagta gagttgttct tctgagattt ttgagacttt ggcttcgtgt tctaaagaga
    12481 cttggctgtt gtggatctca ttgaatggga tcgtatcgga tttcgaccat tcgtccatga
    12541 tgatcgtgtc acattcgata tgagagatcg aacccgcact gtttttggca aaggttactt
    12601 gtcctcgata gtttacttct ccgccgtctt ttgaaattga tttagagacg atagagctag
    12661 atgtatttgg tgcattgtgg atcatttttg cacccgtatc ttggatttgg tttgctcctg
    12721 caaaggcgat ggagagcatg gtgcctcgtg caccttttcc atctaagtaa acacttgggt
    12781 atttcatcgt tgtttttgca cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt
    12841 atgcttttgc tcgtttggtt actaagttgt acacattgtc tgaccagttt tggatcgttg
    12901 tgtaacggca atacgcatct tttcttgtaa agatttctac gatggcggca tgcaggctgt
    12961 tacttgaata agtaggtgcc gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa
    13021 caatgatcag cgtacgttcg aattgccccg tgttttccgc attgatccgg aagtacgttt
    13081 gtaatggaac atcgactttg acgccttttg gcacgtaaat aaaggtccca cctgaccata
    13141 cggctgaatt cagcgctgct aatttgttgt cggttggcgg tacaagtttt gaaaagtact
    13201 ctttgaataa ttcagggtat tctttcaaag cagagtcagt gtcagtaaag acgattccta
    13261 acttttcgaa ttcatctttc atattatggt acacgacttc tgattcatat tgtgcggaag
    13321 caccggctag ataagctcgt tcagcttctg gtataccaat cttttcaaat gtatctttga
    13381 ttttttctgg tacgtcttcc cagtcacgag cgggtttgtc acttggtttt tggtaatatt
    13441 tgatggcatc gaaatcgatg tctgaaaggt cggggcccca tgtttgcatt ggcattttgt
    13501 taaatgtttc tagagatttc agacggaatt caagcatcca ttctggttcg cctttgattc
    13561 ttgacatctc acggatcact tcttctgtca agccttctcc tgtactgaaa accggttcga
    13621 catcatcgtg aaacccgaat ttatattctt ctaattctgg tacgcccatg ttctcactcc
    13681 tcttccttgt ggtaatgatg gaaagctgcg gtaccatcgt tttccattgc ttttcctagt
    13741 gctttccagg caagtgtcgc acatttgatc cgagcgggaa atttagctac accaccaagc
    13801 atagcagcat cgcctaattg gtcgggatct ttgacttctt ttccttggac aagctgtaga
    13861 aagtctacag ttaaatgttc tgcttcttct attgattttc cgatgacggc gtctgtcatc
    13921 atcgaggcac ttgccgtact gatggagcag ccactgccgt caaaggcgat atttttgatg
    13981 attccgtctt ttacatcgac ttgcaattcg atcacatctc cacatgtggg gttgttcaat
    14041 tcgatcttgc ttgtcgagtc agtcaagctt ccacgatgat gcggatggga agaatgatcc
    14101 aagatcactt gacggtataa attgtctaat ttagatagtc ccatgttgga aaaactcctt
    14161 tgttgccatg attgcttcaa ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa
    14221 gctcgcgcga gcagtcgctg atacttcaag gtacttcaat agcggttgcg cacaatgatg
    14281 acctgctctg acggctaccc cttccatgtc taaagctgtc gcgacatcat ggggatgtaa
    14341 cccatccaaa ttgaatgcaa cgactcctgt ccgaaaattc gggtcttgtg gaccataaac
    14401 agtcaatcca tcaattgctt gtaattttgg caacacataa gcggctagtt cttcttcata
    14461 tcgatggata ttgtccatac cgagtttttg caaataatcg attgcatggc ctaatgcgat
    14521 agcgccggcg atatttggtg tccctgcttc gaatttccaa ggtagttctt tccatgtact
    14581 gtcgtataat tcgacaaaat cgatcatttc gccaccaaat tcaacaggtt ccatttcttc
    14641 taaccatttt cgtttaccat ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc
    14701 gctgaaagca aagaaatcac agtctagtgc ttggacatca atggccatgt gcggggcaga
    14761 ttgagcacca tctactacga tgaccccgcc gttttcatgg acaagttcag ctaattcttg
    14821 aacaggattg aagacaccta agacattgga tacatgagca atggagacga ttttggtttt
    14881 gtccgtaatc tttttcgctg catcctccat atccaggaag ccttcttctg ttagttcgat
    14941 atatttcaag acagcacctg ttcgttttgc caactgctgc cacgggataa cattagaatg
    15001 atgttccatg taagagagca ctatctcgtc tcctgctttg atatagcggt cgccgaaggt
    15061 tctggctacc caattcaagc ttgtcgtcgt accacgagta aacagcactt ctgctgtttc
    15121 ttttgcatgg atgaacgccc gtactttttc tcttgcttgt tcataatcat gggtcgcgcg
    15181 ttctgctaac gtatggacac cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc
    15241 taatgcctgg agtacttgtt ttggcttttg ggtcgtcgct gcattatcca gataaactaa
    15301 aggttcatca ttgacgtctt gaaataagat aggaaaatca ttgcgtagct ttttagcatc
    15361 gatcatccat tcaacttccc ttcgattact tcgactaatt ctttccgaac ttgttctaca
    15421 gggattgctg ttaagacaga accgaggaat cctcgaatca ctaagcgctc tgcttcttct
    15481 ttatgcaatc ctctactcat cagatagtac atttcttctg gatcaactcg tcctacactg
    15541 gcagcatgtc cagcagttac ttcgttttca tcgatcaaaa gaataggatt cgcatcacca
    15601 cgtgctttat cagaaagcat caagacacga ctttcttgtt gggcatctgc accttttgcc
    15661 cctttcaaga tgtggccgat accattaaag gtcaatgttc ctttttctcg gatcactccg
    15721 tgttgcaaaa tatgcccgat cgtatgaggg gctttattgg ttactcgtgt atcgattcct
    15781 tggatttgtc tgcccgagct gattgccacg attttgactt ctgcgtgtgc accttctcct
    15841 gctaaatctg agtcaaagtc agcgatcaca tgaccgtcat tcatgacacc aattgcccag
    15901 tcgacagaag catctcgtaa aatgtgccca cggcgattca tataagaagt aatattttct
    15961 cctaattgat ctacggctga atatttgatt tttgaaccag ctttagcgat gacttccacg
    16021 atgatattac ctgatgattt tgctacttgt tctttggttg tctggaaacg ttccagataa
    16081 gaaaattcac tatgttcatc tgccacgatc aacacatgct tgaagaagtg ctcatcggat
    16141 gcgccatctt gaatgaataa ggactcgagt ggttcatcga tcacgacatt tttaggcaca
    16201 tacaggaaaa taccgctgtt cataaaagct acatggagtg cagtcaattg gtcttcgttc
    16261 atgtttactg cttttttcat ataatattct tggactagtt ctggatattc gatcattgca
    16321 gtgaacaagt cggtaaaaat gactcctttt tctgctaatt ccacaggcag ttgttcaaaa
    16381 atcgtccatg aaccttgttg gacaagcaca ggattgtctt tcattgcatc aaatgccgga
    16441 atcgtgcctg tttcactgat ggtttgtgtt tctttcacat caaataacga ccagcgatgg
    16501 aatttcaccc gatcgatatg cggcaatggc agttcatctg ctttcgctag tgcgtcttga
    16561 cggaaggttc tcatccaatc aggttcgcct ttacttaaag aaaaatcatt taccgcgtca
    16621 agataatcga gccagttttt ttctttcatt cttgttcccc cttacgcttc ttcttcttta
    16681 tagtcgattc ctaattcttt actgatacca gcgtagcctt ctgcttccag acgtttagct
    16741 aggtcagcat tccctgtcat gactactcgg ccttccatca tgatgtgaac gacatcaggt
    16801 gtgatatagt tcaataatcg ttgatagtgg gtgatgatca atgcaccgaa attgtctccg
    16861 cgcatttcat ttactccttt agcaacgact ttcaaagcat cgatatccag acctgaatcg
    16921 atttcgtcta aaattgcaaa tgtaggttct aacatcaaca attgcaggat ctcattccgt
    16981 ttcttttctc cgccagaaaa accttcgttc agataacgtt cagccatttc ttctggcata
    17041 tttagtaaag ccattttttc atctaacttt ttcaaaaatg ccataacaga aattttgtcg
    17101 ttttcatcac gtttcgcatt gatcgctgca cgcatgaatt ccgcattcgt gatacctggt
    17161 atctcacttg gatattgcat agctaaaaat aagccaagtc gcgcacgttc atctacttcc
    17221 aagtctaaaa catttttccc gtcaaacaaa atttcgcctt ctgttacttc atagtttgga
    17281 ttgcccatga tagcggcaga taaggtagat tttcctgtgc cgttaggtcc catgatggca
    17341 tggatttctc cggttttcat tgttaggttt acgcctttaa ggatttcttt ttcctcgatc
    17401 gacacatgta gattttttat ttctaagaca gacatgcatt atacccccaa attctctttc
    17461 gttctcactc attttagact aattgagaat gaagcgcaat gaccttcctt actaaaatca
    17521 aatatctttt atttattatt aaacaatttt gatttctaaa ttagaatagg tataaaaaag
    17581 atgagaaata agccattaca gacttatttc tcatcttttt tgttccatac cttattttgc
    17641 atcagccttt actggtttaa cagagccgcc ccatttgtct gtgatccatt cttggacttc
    17701 tttgcggtga agcacttcaa tcagtttttt gattttgtca ctgtcttcat cacctttacg
    17761 agtcgcaata atattcacgt aaggagagtt gtccttttca agcaatacgg catcttctaa
    17821 tggattcaac cctgcgccgt aagcgaagtt tgcgttgatt gcaactaggt caccttcatc
    17881 attttggtac gtagaagcta aaagtgatgg gtcgatcgta tggttgaact tcaagttctt
    17941 agggttttct tcaatatcat cgaatgttgc tgtttccaga tctactccat ctttgacttt
    18001 gactaagcca gcatcttgta gaattgtgat gatgcgtccc cagtctgatt cagaatttga
    18061 agtaatgatt gttgcaccat cttttaattc ggaaacatct ttgacttttt tagagtacaa
    18121 gcccattggt tcgatgtgga cagcaccggc attgacaaag tcatatcctt tttctttgat
    18181 ttctttgttc aaataaggaa tatgctggaa gtaattggca tcaatgtcgc cttcttccaa
    18241 agattggttc ggtaatacat agtcatcgaa tttgacgatt tcaagatcga ttccttcatc
    18301 tttcaataat ggtttcacat gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac
    18361 tttcaatgtc gtgtcgtctg ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca
    18421 tgctgctaat cctacagtca gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat
    18481 atcctacatc cccctatatc ctattatctt ttgtctgttt tcttagttaa gaaatcaccg
    18541 attccttgaa cgataaatac gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt
    18601 agattaccac gctggtagcc ttcctgccaa gcaagacctc caagtccacc tgcgccgatc
    18661 gcgccggcca tcgcagtaaa tccgatcatc gagatcgttg taactgttaa tccagaaatc
    18721 aatgcgggaa tactttcagg tatcaagacc ttccagatga tttcccagta acttgctccc
    18781 attgcatctg ctgcttccag tacaccaggg ctgacttcac gaaaagcaat ctctactaga
    18841 cgggcataaa atggtgctgc ggataaaaca agagctggaa tggctgcttg cgcacctagc
    18901 atagtgccta cgattgcttt agtaaatgga atgatcaaaa ccatcaggat cataaatggc
    18961 gttgaccgga aaatattact gatgatcgat acgattccat aaatgattcg agcatacggt
    19021 ttgtttttgc gtccaataga gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact
    19081 aagaccattg agattaatgt catatatagt gtatcaagga tagcctgatt catcgtttcc
    19141 gggttgattt gtttaaaatc tagatatgtt tctgaaaagc ttttatccac gatggatcac
    19201 ctcaattcct actccttgtt ctttgatgaa cacttctgct tgatctaatt ctttggtttc
    19261 accagtcatc aagacagtca atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc
    19321 atacacaacg ttgacatcga tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt
    19381 ggcatttctt tcgctaaagg tcaaagttgc caaacggccg cttggatttt ctttgatgag
    19441 ttcagctaat agttctgtcg aatcagcttc tggttcgatg tcttgttgta caaagcgttt
    19501 ggttacttct tttttcggtt gtctgaagac agtcaatacg tctccttctt ctacgacttt
    19561 ccccaactcc atcacagcga ctttgttgca gattttgcgg atcacattca tttcatgtgt
    19621 gatcaagacg atggtcaaat tcaatttctt attgatcgct aacaataagt ctaatacttc
    19681 ttctgttgtt tgcgggtcca atgcgctagt agcctcatca caaagcagga tctcaggatc
    19741 atttgccaat gctcgtgcga taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg
    19801 atatgcttgt ccgcgtcctt ctaacccgac taaacgcaac agttcttctg cccgttcttt
    19861 acgtttgctc tttggtacgc cagctaattc cagcggcagc tggatatttt caagtacagt
    19921 tcttgaccat aacaggttga aatgctggaa gatcataccg attttctttc ggaatgtacg
    19981 taattctttg ttttttaatt ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt
    20041 gggaagctca agtccattca ggagacggac taatgtactt tttcctgcgc cagaatagcc
    20101 gacgattcca taaatatccc ctttttcaac gtttagtgaa acgtcattga cggcttggat
    20161 gtttccgttt ttcccattga atgtcttttt gacattcgtt agttggatta atgacatgcc
    20221 attcacatct ttctcttatg atttttcaaa caaaaaagcc ttcgccctct tgccttaagc
    20281 aaaaggacga aagcttgaac ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa
    20341 cagcctcttc cagtacttgg acttagcttc tttccgtata ctgtcgcgct gtaacgggca
    20401 ctcccgtagt aagtttgctt acgctcgctt actctgctct aagaccatct tccatctcat
    20461 tttcgttacc cattttcagc aaacagggct ctcttcaaac gtccgtgaca tgtactcttc
    20521 ttttcaaagc tttctcgttt catataggat tgcttatgat tatagcaact ggaagaatca
    20581 gtgtcaactt ttttcaagtg attttctgat tttataattc gtcaaattct tttggatcaa
    20641 catctttgaa acttaagatc cacgcattca tttcatctga atcattcaag atattgatat
    20701 cttcaccgat tttatcatta acagaagaaa ctgtacccgt tagcgggctg gcaaattcat
    20761 tgaccgcttt ttccgcttca acttcgatca gaggttcatc tttttttagc gattgtcctg
    20821 cttttggcag attggcaaac gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac
    20881 caacacagta ttcttttcca ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct
    20941 tgcctgccat tttatatcag ttctccttca taaatttttt cattgaatcc aattgcaaca
    21001 agctggtcct ctcgtaagat cagtggtcgc ttgatcagca taccatctgt cgataagact
    21061 tggctcgctt ttgaagccgt aaactcgcct acttgatctt tcagtccaag ttcccgatat
    21121 ttcatcccgc ttgtgttgaa caggcgacgg attggtactt gactcgtttc tagccaagtc
    21181 tcaatcgttg aggccttcgg tacttctttg atcatatcaa cggtttgata actaacgcca
    21241 tgctggtcta accacgcttt ggcttttttg catgttgaac atttcgggta ccagtaaaag
    21301 gtgtacaatt ttcttacctc cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg
    21361 ataatatcaa cccgataccg atcatgttcc ctaaaatcga ggatcctccc tgactaatga
    21421 aaggcaatgg gatccctgtt aaaggaagta atccgatatt tgctccaata ttctcaaaga
    21481 catggaacag cagcatcatg atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg
    21541 tatcgaaaca cacccgaatc atacgataga ttaaaataaa atacaagaaa atgacgaatg
    21601 caccaccgat aaagccgaag ttttcaccga taacggtaaa aatcatatcg gactcacgaa
    21661 caggcacata gatattgctt acgttgaatc ctttaccaaa caagccaccg gtaccaattg
    21721 ccaaaagtcc ttgggccggc tgataactca ttcctgacgt atcatggaaa gggtccagcc
    21781 atgaatcaat acgtgcaaac tgataagatt tgaatccaac tttataaagc aagtctcttc
    21841 cgccttcagt cgtaaccaag aagatcgttc ctgctccaac gactactgcc aagattacta
    21901 caggtgcgat gatgcgccaa gagattccag acatcaggaa aatcccgcca aagatcgcaa
    21961 gaaagacaag catcgttcca aagtcatctt gggccatgat caatgctaaa accggtattg
    22021 tcacgatcaa gagcttccca atcagccaaa agtccgattt catcgtccgt tcttttactt
    22081 gcgtattatg tttagttacg atcagtgcca tcatcaagat gtacgcaatc ttcatcaact
    22141 ctgctggctg gaaagtaaac gcaccgaatc gaaaccagtt tttagaccct gttgaagcag
    22201 ctaaattctg atcatagaat ttgagcaaga gtcccattac cactaacccc aaaccataaa
    22261 ggtaaggagt cagtttccag atccatttgg aattcaaatg catgatgaca acgattgctg
    22321 ccgctccaac taaataccag acaacttgca tccctacgcc tttcaagact gctggattgt
    22381 tcggatcatg gctaagtgct acatataatg agagcagacc aatcaaacat aacagaaaga
    22441 ctggtaaaat cacgccataa tcaatacgat tatcgttcga agcttttaat tttttttcca
    22501 tgtttcatcc cttttcttaa aaaccatact gctgattatt atagcggtcc agttagataa
    22561 tggaaagtct ttccatagac atttgggaaa aattaacttt ccgtttgtgc agttgcttga
    22621 gcaaatgcag tacaaaagcc tactgcttat ctttagtgta tcatagaact ttttttgata
    22681 catgcaatta tctaccagct gagattctcc tcgctgtgag aagtttctgt gctttcattg
    22741 tatcgaagga tctcttcggc aatctctagc agctgatcag aaaccagatt ggttcgttgg
    22801 atgaaataaa aatatcgatg gaaattcaac ggagtcattg gcacattttc tggaacagag
    22861 cgcttagaca aaatcgagcg accgattcct tccttcagca acgcaacaat tacatcgtta
    22921 cttttcacga tcatcttctt atcttggata ttcatctctt caaaataacg ctgcgtgtaa
    22981 tgaaaaacac cagagtttgc ttctcttaca agccacaatt tgctttttgg gtccccagcg
    23041 atgactagct ggtcttccat caaagagtat ctttgtacgc cggaagtctc taatggcttt
    23101 tcgataaaac caaaatcgga ttcatgtttc gctacttgtt ctaatgattc atgagaattc
    23161 gtgatttcga tctcaaaatg cactttagga aaccttttga tcagatgttt catcaactga
    23221 ggcaaaagat agacagcaaa ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt
    23281 tgttcattga gcaatttttg ctgagtttct tcccattctt ctaacaaaac cagtgtccgt
    23341 tcatataata agtcggcttg cttagttgga agcatctctt ttcttccgtt acgttgaaaa
    23401 agatcgatat tcaattcccg ctccaactgt ttgatttgat tcgagactgc tggttgagat
    23461 aaaaacagta cttctgctgc tctggaaaaa tttcttgttt catatactgc tcgaaaagtc
    23521 gttaataact taaacatggc accacttcca taacttttta tgataattac tataaaaact
    23581 atttgattta aatatacaaa aaaccatcta taatagaaat gtacaacaaa acagttgctg
    23641 atccaactag taacttagat gaagacaatc agctgtctgc ttgctcgtaa tcatacattc
    23701 ctattcattt tacaatgtat tcgttcatct tggtactaaa atctattaaa taaaaaggag
    23761 aatattatat atgaagaact ttttgaaaat tattccgatc ccgatctgcg ggttgatcct
    23821 tgggttagct tcccttggaa atctgtttaa agactatcac catgttgctt taggcaatct
    23881 aactggcggt atctctatga ttcttatgat attcattttt gttaaattgt ttatcttatt
    23941 cgaacacacg aaacaaacgt tgcatgatcc aatcatcgcc tccgtttcac caacttttac
    24001 gatgtcactc atggttattt gtacttattt tgtttcatat gaaagtattg caccaattgt
    24061 caaattcatt tggctgtgcg ctgttatctt ccaagtgatc cttgtctttt atttcaatta
    24121 tcaccatgtt atcaaagctg atctttcaat tgaagcaatt tatccaagct ggtttatcat
    24181 ttacgttgga tttggggtca tcactgtaac tgccgggaac ttcttcccac ttattggaaa
    24241 aattttcttt tggatttcat tgatctgcta tgcgttctta ttgccgatca tcatcaaacg
    24301 tgtctttttc gtaagaaata tggctcatcc gacacgtccg ctgattacga tcgttgcagc
    24361 acctggttct ctttgcctaa caggttatct taaaaacttt gctgaaccta atttttatct
    24421 tgtcgtagca ttgttcgctt tatctcaagt attgtatttc gttgtgctgt ctatgcttcc
    24481 acgtttgttc aagcttcgtt tttatccaag ctatgcagcc tttacattcc cgcttgttat
    24541 ctctgcaaca gctatattta ctactgttca ttacttcaac agtttaggta tctatagtag
    24601 cgtattggat ttcttaaaag ttgcggaatg tctgtttgca agtgtagttg tcttctacgt
    24661 tcttgctcat tacgtacgtt tcttaattaa agaacatagc aaacatggaa aatttgtttc
    24721 taaaaaaatc gcttaataat cgattcaata ggttgtgaaa aagctgttct gcatcaagta
    24781 ataagaacgg ccaaacaaaa acagctgttc atgtttttgt actggttggg cttattaccg
    24841 cagatgcaag cttttgaaca ccgtttacca agaggtcgta aaagaagtgg tcagcttcaa
    24901 gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt
    24961 ccgtagaagc tacttctgga acaccgttta ttcgaaacaa gaggtcgtac aagacttttg
    25021 tcacgacctc ttgttctttt gtcaaaatac tggtttagtg ctactatgtt cgagtatgct
    25081 tttagtattt attgacgaaa ggaaccgatc aaatgaaatc attgtcacat ccaataaaat
    25141 ctgtaaagga ttcagcggtc cgcgaaatct tacccggttt actgctttct tttgccatcg
    25201 ctgccttcag caggcttctt gctatttatc tgccacagtt aggtggagcg actattgcta
    25261 ttttattggg tattttatta ggaaatactt tttttaaaca gcctgttcta gcaatcggca
    25321 caaaatttgc tgaaagcaaa ctattagaat tctctgtttt tttactaggt gcaactgtca
    25381 cagctcaaac gattgctcaa attggtgtaa aaggaattgt ttttgtgtta ctgcaaatgt
    25441 cagtcactat catcggtgct tacttgattg gtaaaaaact gcaattcagc gatacttctt
    25501 ctttgatgat ggctggagga aacgctgttt gtggttcttc tgctattgct tcaatcgccc
    25561 cttccatcca tgcgaaagag gaagaaaaag gacagatcat cacactagtc aatctactgg
    25621 gtactgtgat gatgttgact ctcccttttc ttggattaca gttgtttggt ggaaatctca
    25681 ttgaaaaaag tgcacttttg ggtggtacac tccaatctgt tggtcaagta gtcgctgggg
    25741 caagcttact tgacaattct gttgtcgaat tcgccatgct atttaagatt ctacgtatta
    25801 tgatgctcgt tgttgttgtc tatctgtttg aaaaattcat gcacaagaaa caaccagctc
    25861 tgtttgaaac gccaactgat acgcagaaaa aacgaaaatt accgcttcct tggtatgtat
    25921 tagggtttgt gatcttttgt ttggtcaaca gcttgttttc tttacctgta gttattgacc
    25981 aatcggcaca ttttatcagc agctggtttg agatcactgc tttagcggca attggtctgc
    26041 gtttagactt tgcaaaattc atgaaagaag gtacacgctt cttatcttat ggattagctg
    26101 tcgggcttct ccaaacagtg atggctttac tatttatttg gctgatcaaa gtttagaatg
    26161 aacatgaaaa aacagttgtg attctcgcat ggaacaatgt ccgaatcaca actgttttta
    26221 attttgcaga tatccttcta acaacaacaa ttggcgtttg atttcaaccc catctattgc
    26281 gtttcccata tatcctgtca gttttccatt ttgcccgatc actcgatgac aaggaataac
    26341 gattggcaat ggattggttc gatttgcctg cccaatcgct cgcacagctt tcggactatc
    26401 aacagcaacg gctacttctt tatacgttcg gatttctcca taaggaatgg tcgacaaagc
    26461 tttccagaca ttttgctgaa aaggcgtacc tctctttata gataaaggaa catcaaattc
    26521 ttttcttttt cccgcaaaat attcttcaaa ctgttcttgt gtgcgcgtta aaattcgctg
    26581 attcgctgat tcgctttctg aaattggctg aaaagataca gcagtcaatc cggattcatc
    26641 tgcatctaac cataaaaagc cgataggtgt gtgtattttc attttgtttg ctcctagtca
    26701 atagactgga acttcctcct ttttctctat ccaaaactac ttgccaacta ataaaaaatg
    26761 ttcttaaaag tttgataaac cgaggaaaca tggtaaacta aatacgttag tttacaaaaa
    26821 gaatacctgt tcttttacgt aaaatcaaat tttaaaggtg ttatttaagg tttattaaat
    26881 tttgaatgta aaccatatat aaacatacga ttgaaaggat agaaacgatg aaagtatcaa
    26941 ttgttgtccc atgttttaat gaagaaaaaa ctgttcctct tttttttgat gaggttgaaa
    27001 aattcaaagg tgcccatgag ttcgaatatt tgtttattga cgacggatca aaagacggta
    27061 cgttagcagc aatcaagaag ctagcctcag cttatcctta tgtccgcttt gtctcatttt
    27121 ccagaaattt tgggaaagaa gcggctctgt acgctggatt gcaagcttct acaggagatt
    27181 tagtgaccgt gatggatgtt gatctgcaag atccgcccga actgcttcct gagatgatca
    27241 accggatcac tacttctgat atcgattgtg tcggcacccg gcgttcgact cgtgaaggcg
    27301 agcctgcgat acgcagtttc tttgcgaaaa aattctacca gttgatcaat aaaatcagcg
    27361 atacagaaat ggtcgatggc gctcgtgatt tccgcttgat gactcgtcaa atggtggatg
    27421 ccgtcctaga attaacagaa tacaaccgat tttcaaaagg attatttagc tgggtcggat
    27481 ttaaaacaga atatatttct tatgaaaacc gcgaacgtgt ggctggcgaa acatcttggt
    27541 ccttctggaa attattcaac tactcgattg atggtatcgt gaacttctcg gatgcaccgt
    27601 taacgattgc ttcatttatc ggcgctttgt cttgtgtcgg ttctggtatt gcaattttgt
    27661 tcattatttt gcgggcattg ttgtttggtg atcctacatc aggatggcct tccatggtct
    27721 cgatcttcct gttcattggt ggaatccaat tgttatgtct tggaatcatc gggaaatata
    27781 ttgggaaaat cttcttagaa acgaaacagc gtcctgttta tatcataaga gaaacagaaa
    27841 aagtgaagcc aaatgaattg aagcctcacc aataatatac gtcaaatgaa gtaaacattt
    27901 ttttaaatga aaaaggactg tgacaaaagt cttgtcacag tccttttttc cgaataaatg
    27961 gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt tgagaagcaa
    28021 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctatcacaac ctctttcata
    28081 tcttattact tcttcattgc tgcttttagt gctgctgcta atgggctttc ttctggttct
    28141 tctgcttgtg catatttttt cagcaagcgt cgttcttcat gtttggtcat tttcttcttc
    28201 tgctcttttt tatcaagcaa tttttctgtc gttccatcgt atttacaacg gaaataagca
    28261 ccgtttttac cttcgatgat ttccattttc cggtgacatt gcgggcatcg atgattagag
    28321 acttttggat cttttcttct gcggtagttg caatctgaat tggtgcagac atagattttg
    28381 ccgtcacgtg tgtttttctc gcgcagattg gccccacatt ctgggcattt cttttgggta
    28441 atcgagaaat cttgatattc ttgtttgctt gatttgatct cattcaccaa gcgttttgtt
    28501 tctttttcga ttccattcaa gaaggttcct gcttgtttct ttcctgcagc aatagcttca
    28561 aggtcttttt cccagctttc tgtcaaatct ggtgttacta aagagggatt taccaatact
    28621 aataattgct tacctttagg agatacttgc agtccttgag gggtacgttc catcagttca
    28681 gatttgatta gtttctcgat aatctctgca cgcgtagctg gggttcccag actgtatttt
    28741 tccatttgac caagcaagct tgcttctgac aatggtttag gcggtgcaga tagttctttt
    28801 ttgatggcga aatcgggatt gattctcatt cctttttccc atttgactgt ttccgtctca
    28861 gtttcagttt cgcctttcca tcctgcttgg acgacacgat tttgccgaaa aatgaatcgt
    28921 tctttaccaa attcaaccgt aacttttgtt tggctgactt tatggggttg agcaaacagt
    28981 cctaaaaaac gagagacaat catttgatag atttttcgct cttcattact taatttttca
    29041 taacgtgctc tgttttcagt tggcaaaagt gcatggtgat cggtcacttt actgtcttgg
    29101 aatacttttg tctgttggac gatggctccg tttttcaaat actgcttgac ctcaggagca
    29161 aagtcgctga ctgcttgcaa tcgttctttc atcgtacttt tcatgtcatt tgtcagatat
    29221 ttgctgtctg tacgcggata agtaacgatc ttatgggtct catacaaact ttgtacaaga
    29281 cttaatgttt tctttgcaga atagccatag cgttgattgg cttctctttg gatctctgtt
    29341 aaatcataag gcaatggcgc agattccgtt tttaattttt cttggacttc tgtgacagtt
    29401 cctttgcttg tactgatcaa ttgaacaagt ttttgtgctt cttcttgtgt ttttaaagca
    29461 aattgatttt tttgaagcat tttggcttgt tcttttccag cttgcaacgt gatcgtgaaa
    29521 tacgtttgtg gacggaactg ctcgattttc ttttcttggt ctctcactaa tgctagtgtc
    29581 ggtgtctgca cacggcctgc tgataaatta tccgcgtact tcacagttaa ggctctggtc
    29641 acattcaatc ctaccagcca gtccgctttt gcccgggcaa gagcagattc atacaatgca
    29701 tcatattctt tcgcaggtct tagttgcttg aatccagttt tgattgcttt atctgtttga
    29761 gaagagatcc ataaccgctt cactggttta ttgaaatgca catactctaa gatccagcga
    29821 gcaacaagtt ccccttctct tccggcatct gtcgcaatga ctgcttctga gacatctttt
    29881 cgctgagcca gctgcttgat tgcttttaat tgatgtcctg ttttaggaag tggtttgatt
    29941 cctagttttt tagggatcat tggcaacgtg tccatttgcc agctctgcca ttccttattc
    30001 agatcttccg gcattttcaa tcctaataaa tgaccaaggg cccaagtaac gatcaccttg
    30061 ggtccttcat aataattttt gtgcttttga ttagcaccaa gcaccttgct taggtctttc
    30121 gcaacgcttg gtttttccgc tagaattagt tgtttcataa aattcctttc tgctttctac
    30181 tgtgatgggc tgatgatttt tcaacaacgc aagtcccttg tattgctgta aatcatcgtc
    30241 acactcttca caccagcgct catctccgtc attccaaaag gcgctgagaa agttactttt
    30301 tgcattgata tattcataat tttgatggat ttcgttccat tgtttttcgt acgcttgtcg
    30361 tgcttcttcg aaagactcga atgtttcttc ttcttcaata tcttcttccc agtcttcaaa
    30421 aaaccaccaa ggctcattgt ccccgaacat ggtaattact tgatacatac tcgtgctcct
    30481 tctttcagct tttctagact catcttagtc acttttcaag aaagttgcaa gaattttctc
    30541 ttataaaagg gtcatttaat gaacatctta aaattgttac aaatgaatct gattgtttaa
    30601 ggcaaagtag cgataaagtt attgatcatc caaaaatagc tgtcttctgg atcgattgtt
    30661 ggttcttttg taaagtatcc taaaaaacgt aaggaaagat aaccgtggat catactacga
    30721 agttccctgc ttcgatgaac tttttcttct ttcgttaatt gaaaagcctc aaatatctgc
    30781 aaaatgatct gattcgtttc atggattcca tctaataaaa cagcttcatt ggtatgcggt
    30841 acactgatca gtaactcata cactgcttga tttgaaaaag caaaggatcg ataagtttct
    30901 gcatagatct gcagtgcttc ctgaccactt tttcccacaa gagcccgtcg cagctggtca
    30961 ttaagttcct gcagaagttt cgctgttagc gctgttttga catcacgaat atttttgaaa
    31021 tggttataca gcgagggagc cttgatatcc aagcgtttgg ctaaatgctg aaaggttatt
    31081 tgctgaacat ccatctcttc tgctagttct cgaaagcagt cgatgatttt ctcttgagac
    31141 agatttcttt ttttcatttc catgtttcct ctcataaaat aaatccttca ctctttcagt
    31201 ttaccacgaa aaagaaagag aaaaaagaga ctttcattga taaactacat attgtagttt
    31261 ataataaaga gctgtagatg aaaattttca gaaagaagga gtctaattgg ctgcaagaat
    31321 aaaggaaaaa ttatttagga aaagagaaac ttgggaacaa aacttgtttg tcttatggtt
    31381 tggaacattt atggctggta tcggatttag tttagtcatg ccttttatgt cactttacat
    31441 agatacgtta ggtaattata gtacgtcaca attgaacttt tggagcggat tgactttttc
    31501 atctactttc ttggtgacta cgcttatctc tccttggtgg ggaaaattag ccgaccaaaa
    31561 aggaagaaaa ttgatgctgt tgcgtgcttc attaggaatg gcaatcgtca tttctttgat
    31621 gggttgcgtg actagtgtgt atcaattagt cgcattgcgt ctgcttcaag ggattttttc
    31681 tggttatatc agtaatgcca ctgcccttgt cgcaactggg acgccaaaag aaaaaagtgg
    31741 tcaagttctt ggaaccttag caactggttc tgttacagga acattacttg gaccgcttct
    31801 tggcgggatc tctgcttcag cgtttggcta tcgccctacc tttttcatta cagggatcat
    31861 cttatttttc gtcttcttgt tgagtctgtt tttcgtccat gaaaaatttg tcccagtgaa
    31921 aaaagaaaac atggtttctg ccaaacagat tttcaaagac ttgaaatatc cacacgtcgt
    31981 catcggtatg ttcgtcacaa cgatgatcat ccaagcatcc aacaattcca ttagtccaat
    32041 catcagtctg tacattcgcc aattgctgca tggacatggg aatgttacgt tgatcagcgg
    32101 aatcattgct tcgatacctg ggattgccac tttgatagcc gctccaagat ttggtcgctt
    32161 aggtgacaag attgggagtg aaagaatact tgcgattggt ttgggttttg cgattctggt
    32221 ttatatccca atggcattcg tcacgaatgt ttggcaatta gctgccttac gttttttgat
    32281 tggtatctct gatgcttgtc tgctccctgc tgtccaagca ttgatcacaa aatattcgcc
    32341 tcatcaagct gctggacgga tcttcagtta taaccaatct ttccaagcaa ctggtaacgt
    32401 agtagggcct atgatcggct ccagtgtttc cagtgcattt ggttatcgtg gcgtctttct
    32461 ttcgacttct cttcttgttt tggtcaactt cttgcttgta cgacacaata cacaagagat
    32521 ccatcagcaa gaaactgcag aagaaaaagc ttcaggagtc tccatagctt cagggcactc
    32581 tccttctcat agacaataag cgtaaatgaa aaacactgct tgaacagttg tcacagactg
    32641 tccttgcagt gtttttgttt aggcctgttt tttcaagaac tcagataaga ggaataatcc
    32701 cttttccaag gtttcttttt tacaacagta acctagtcgg gcatgcttcg gtaaatcaaa
    32761 ggcacttcct ggtactaata gtactcctgt ttcttctaat aaattgatac aaaatgtctg
    32821 gtcatcttct tcgatggcta atttaatgaa agaagtagag acgtaattgg gagcgacaag
    32881 ttcaactttc ggttcattgg ctacccattg ttttaaaatc acaaggttct ctgtaatgat
    32941 ctttttgttt cgcgccaata ttttttcttt attcttcaat gcatgtacag caagatcatc
    33001 tgacaggact ccgccgcaaa tcatcgtgta gtctcgatac tttcgaaaaa cttctgccaa
    33061 ctcaggtcca gtagctgtcc agccaatacg aatacctgga atggagtagg ttttggatag
    33121 ggaattggtt gctatacctt tttcatagac atctacgatc gacaagaatt cacctttatc
    33181 cgttaaaggc gcatatactt catctatcaa tacatatgca tctactgtac gagcaatttc
    33241 tgcgattttt tctaaagttt tccgatctag caatgtgcct gtaggattgt ttgcactatt
    33301 caaacagatg atttttgttt caggtttgac taaggcttct agctgttcta aatcaaactg
    33361 ccaatcttct tcttccttca aatgaacaaa gtcaacagcg gcacctaaag atttggggat
    33421 gtcatataat tgctgataag taggaagcat cgaaacaaca tggtctcctg cttccactaa
    33481 cgcgtataga gctaaaagat tagcaccagt cgctccattc gtttgtaaga tgttttccgg
    33541 atcgacggtt tgataaagag aagcgacttc ttgtttgaaa acatctgatc cttcgatcca
    33601 gccatagtcc aatggttcgt ttttgcgttt ttcgaaaaat ttacttacgc tagtcccatc
    33661 taagccaacg agttcgtcta atgtcaatgc ttcgatcgag ctttgtgcaa tatcatatac
    33721 cgctttcttt tcccatttat ttagccattc ttctacaccg aaatttgcga ttttcatgtt
    33781 tctttccccc ttatatcttg atccagccta cagcaagcaa cccgattgaa agcagtgcgg
    33841 ccacacaaat cagcccaatc attacttttt cttttcccgt aatttgatgt ttggtttcac
    33901 gacaagcgat ccagtagaag caaaaaccag gaagataggc aatactacat aaaaggacat
    33961 aagaaaatcc tgctaaaatg atgcccatca attcaaaaac tacagcaatc ccaccgatca
    34021 ctacttgttt tttgattcct tcttttaaag aaaactggac ttgatagatc ccgacaagta
    34081 gataacaaat caaaattgcc gctgtacata aagaataggc aaattgatag gcataatcag
    34141 taactaagaa agtcaataag aatagattag tcaagattgc agtaacaact aacgaataag
    34201 ttggggcatt ttttttattc gtttttcccc aacctttcgg caacagaccg tctcgagcca
    34261 tcaacatcgt tgtttctgct gggagcatcg tccaagacaa ccaagaacca atgatcgaaa
    34321 taatcaagcc gatattgatc agcgctgctc cccaatttcc cacgattcct tttaaaatat
    34381 ttgccatagc aggttgtgaa atagttgcta actcttcttg agataagacg ccgtatggca
    34441 agatcgaagc aaggatatag ataaacaata aacaaagcag accaatgatc gatgcttgct
    34501 gggcttctga acgtttcttc gcgcggttgg ctaaaacact tgcgccttcg atccctacaa
    34561 agacccacat cataaccatc aaacaacctt taatctgcaa ccagatcgtt ccttgctgcc
    34621 ctgttccatt cactagatta tcagatacgt taccccaaaa atcagcagtg aatatattca
    34681 ctttaaatgc gataaataca aatattaaaa ataggaataa tggcactaat ttacagatcg
    34741 tcacgattgt attgatgacg ctcgctcctt caactccatt gtttactaat aaagtcagac
    34801 cccaaataaa aatacttgca aataaaatag aaggaacatt ttgtccacct ttgaatatcg
    34861 ggaaaaattc tcccatggcg ctcatcagca tcgttgcaaa agcaacattt cccagccacg
    34921 cagacaacca gtaaccccaa ccactgatga agccacctaa tttaccaaat gaagcttccg
    34981 cgtaaccgaa aataccacca tctagatctg gacgcttttc tcctaaatga ttcagcgata
    35041 ataccaaagc taaaatacca atcccaacga ttatccatga aaggatcgct ggccctggag
    35101 ctgcagaact agccagatca cttgtgatcc caaacacacc gcttccgatt gacgaactga
    35161 caaccagagc aaccagtccc catttgctaa tacccgtcga tttcttttcc atttccatca
    35221 ccctcactta taaaattgaa caaatcactt gcaaaagatt cccttctatt ttgcaactgc
    35281 tcttgcttaa caaaaagcaa ctttattcat attattggat attatgaact ttttacacaa
    35341 gtatttcaag tataaaatga ataaaaatta attttattgc aaaaaaacag ctatgctatc
    35401 caaaagaaaa ggtgtttctt ttgttagcca tagctgtttt gattcattta atatcgctta
    35461 aacgtattgt aaaacttttg tttcttgata tgcgtggtcg atcaaaccac cacccagaca
    35521 ttccattcca tcataaaata caactgcttg tccaggagtg attgcacgta caggttcgtc
    35581 gaatacgact tctgcacgat tatcatccaa taagcgaact gtcacaggaa cgtcttgctg
    35641 acgataacgg aatttcgctg tacacttgaa ttcttttggc attggttcat tcgtcgtaaa
    35701 gtggatttca cttgcatcca agcttgtggc atacaatgca ggatgatgga acccttgccc
    35761 cacatataat gtgtttgtcg ctagatcttt tcctacaaca aaccagggtt cttgcgaatc
    35821 tcctccgcca ccgatcccta gtccttgacg ttgaccaatc gtataataca tcaagccagc
    35881 atgttgtcct ttgacttcac catcaagagt gaccatattt cctttttttg ctggcagata
    35941 gttgctcaaa aattgtttga agttcttttc accaataaag cagattcccg ttgaatcttt
    36001 tttcttagca gtcgcaaggc ctgcgcgttc agcaattgcc cgtacttctg atttttccat
    36061 gccgcctaga gggaacatcg tttttgaaag ctgttcttgt gataattggc taaggaagta
    36121 agtttgatct ttattattgt ctactccacg aagcatgtgg accacgccgt tttcatcgcg
    36181 agtgacttgc gcataatgtc cagtagctac ataatcggcg ccaagatcca ttgcataatc
    36241 caagaatgct ttgaatttga tttctttgtt gcacatcacg tctgggttag gtgtacgtcc
    36301 tgcgcggtat tcggctagga aatattcaaa tacacgatcc caatattctt tttcaaaatt
    36361 cacagagtaa taaggaatcc cgatttgagc agctactttc gctacatcct tataatcttc
    36421 cgtggccgtg catacaccgt tttcgtccgt gtcatcccaa tttttcatga atattccgat
    36481 cacgtcgtat ccttgttcct ttaacaaaag tgctgttaca gatgagtcga caccgccgct
    36541 catccctacg acaacgcgtg ttttgctgtt gtctgtcatg atatcaccat catttcaatt
    36601 agattctgaa acatctacga gttgaaggtc gcaacttttc agtattttac attatacgtc
    36661 atatttctga aaatttcatc ccttaattat caagtcgtaa aaatactgtt ctactggacc
    36721 tattcagtac acaaacatga tagtcaacca aaaaatcact catcttttga gcattcgata
    36781 taaaaaattg gcatactcat aaaaagggcc agcctttcgg caataaggaa taattttata
    36841 aattgtgaaa aactattttt caaaattgct tcttatcgct taaagctgca cgtctcattt
    36901 acaacctttt ctttttagat gttatgagtt gctcagataa gtgattttgt ttactctaag
    36961 aaaaaaggga ggcaaaagat acatgaatta ctttatcagt gatatgcact ttttccatga
    37021 acgcttatta ggaaaaaacg actttgctcc gcggctcttt gcaaatgtgg atgaaatgaa
    37081 ccaacagttg atcactagct ggaatgcagt aataaaagaa accgaccatg tttatcatct
    37141 aggcgacctt gctatgcatc cgcaatatga aaaagcaaat gcagaaattt tggaactggt
    37201 cactcaactg caaggaacaa tccacttgat caaagggaac catgacagtc gcgctttttt
    37261 ccgttattta gaaaaaaatg atcctaggct tactgacgga agtcccaaat tttattttta
    37321 tgatgtaggt gccatcgtta aattcgacca tcaacaatat tacttgactc attatccgat
    37381 gttgctcggg cctaacgaca ctatccgcaa tcttcatgga catatccatc attacagcgt
    37441 acctatcgag aacgatgtca atgtaggcgt agacgcaccg gaaagagaac tcttaaagga
    37501 aaaactacca tttggcactc cgttatctga gcaacaaatc aaagaaatct atgaagcaaa
    37561 gaggaagtac gataggtcgt gaaaaaactg ccctatatca agtattaaac aaataaagat
    37621 aattactcat atttttcatt tgagtaggct tattgccaca ggaacattag gatcagaaat
    37681 ggagttgtaa caagacttct gtcacaactc cattccatac tattcgtctt tttgctcatg
    37741 gtgttctttt accagttttt tctgtaattc tttatcataa tttcgggcag ctgacgttaa
    37801 aaataaaacg attccgcata ataaaatccc gattccggaa aacaagaaca ttttttctac
    37861 ccctaaacga tcagctaatg gtcctgcaaa taataatcca attggacctg tcaggttcat
    37921 caaagagttg aagttcccta agacacgacc taaaacattc gattcataac tctgctggat
    37981 cattgccatc agcagtgtat tgaaatatgg cgttgcacaa cctgctcctg cattcaagat
    38041 aagaaagtac aagaaccctt gactatcgtt tggaaccaat ccgcttgcac cgatcgtcag
    38101 gcccataatg atgtaggcta taataatagg tttcattcgg tctttccatg ttcctataaa
    38161 actgatcagt gcaccgccta agagcattcc gactgcgtag actacttcca ctgctcccgc
    38221 ctgtccaacg gaaccaccaa agtaatctag cgtcatcaat ggatacagac tgattgctgg
    38281 catgaacaac agcatcacaa atgcaccatt gatcgttatg taccataacc ctttattatc
    38341 catcaattgc tgcagcccaa attttgcatc tttaaaaata gtaagcggaa cttccaatat
    38401 ttcaggagaa acttttggga tcttcacaaa aatcaataag ccgacaccaa aaactgcccc
    38461 caatacatct aataaaataa ggtaattgac aggaacgact gaaaacaaag ccgcacctag
    38521 agctggcgca atgatgaaat tcgctgattg gaccattcct aactgtccat tcgtccttgt
    38581 tatgtgagaa gatggaacga ttgtcggcat aatcgactgg atcgttggca tttggaatgt
    38641 ttgtgcgacc gaacgcataa acaaagacac aaaaactagc caaatcggga aggattctga
    38701 aatcgttccg actactgcta aaataagcgc gaaaatcgct acaataatat ccgtgacaat
    38761 caaaagagct tttttatccc agcgatccac tagcggtcct acaaatgggc ttagtaaaac
    38821 catgggaatc attcctagta aagtagcaaa actcaaaatt gtcgccgaac cggtttctct
    38881 tgtcaaatac cagatgatcg catattggac gaccatacta gtgattcctg acaaaaactg
    38941 tcccgataaa aagaacaaat aatttctctt ccaattcgtt aaaacgaccg actgttcatt
    39001 ttccataaac atccctcttt tctttttcat tttgtatagc gtttgcacaa caatcattat
    39061 acgggattct tttttcaaaa aatacctttt tccctaaata acaatcgttt caatttaaat
    39121 ttctttactt agaaacaata aatcagcaaa atattaaaaa taacaacatg tgttaaattt
    39181 aaaaataaaa aagacaacta atttgcgtcg tccttacaaa gttagtcatc taacaatagg
    39241 ccgtttcaaa actagttgtc tttttatatc cacagatcat tcgacttttt cgaataaccc
    39301 acggctaatc atcccatcca tcagaaaata aaatttatct tggctcattt tctgttttgg
    39361 atctttgaaa atattgactg ctttcaatcc atttgctgta gtgatcgaca ttttcaatgt
    39421 ttgaatatct tttgatacag tgatttttaa tttgaatcct tctttgcttt gcggcgtggc
    39481 atctaatggt cggatcaatt gataattgcc tccgtttgtt tctgtaaatc cattatcgcg
    39541 caatgtatat ctttttgcat caggatgcaa acgatagcta attggtgcgc ccagtaccgt
    39601 tgctgttgtt tcaaatgcca tgattttcac ctcgataaaa ttagtttctc tttattataa
    39661 aagtttatcc tgatttttac taggtttttc agttaagttc cctaaaaaat gaaggcgttc
    39721 tctcagacat ctgttcatat aagaatgact tcactagtta ttttttgaca gcacgaacta
    39781 attgatcaag gaaatactgg atgtcgtctt cggtatttcc caaaccaaag ctgatacgga
    39841 tggattcttt tagtatcggt gtatcttctc catacattgc ttccaatacg tgtgaaggct
    39901 cgatatttcc tgctgtacaa gctgaaccag tagaaatagc gaccccatta agatcaagct
    39961 tcatcagtag caaatcattt gcttcgccaa ttaagcgaag attcaacaca tgagggagtt
    40021 tattcgttaa atcaccattt acttgataag ggatttcgtt tgcttctaat gtatcaagaa
    40081 tcaattgctg gaatccttta tatttgttgt tacgtgcttc acgttcttca ggtgacagca
    40141 gttcaactgc ttttgccata ccccagatcc ctgctaaatt ttctgtacct gcacgccgct
    40201 tttcttcctg ctccccgcct aaaagcaatg cagggatatt tactccgtct cgtttatata
    40261 agaaaccgac gccttttggc ccattgattt tatgagcaga gatacttaat aaatcgattc
    40321 ctaattcttc tggatggatc gtttggtttc cgtacgcttg taccgcatct gtatggaaat
    40381 aagctggatg atccgctaaa agttcaccga tttctttgat tggcatcaaa tttccgattt
    40441 cattgttccc gtacatcaca gagaccaaga tcgtatcttc tcgtaacgct tttttgacct
    40501 cttcgatcga cagattccct ttttcatcta caggcagata tgtaacgtca aacccctttg
    40561 tctctaaata tttcatggta ttaagaacag caggatgttc gatagctgta gtgattagat
    40621 gtctgccttc tttttgacgc gagaacgctg tttctaagat agctgtatta tccccttctg
    40681 ttcccccact gttaaagatg atctcgtgag gttttgcttg cagactgtct gcaatgattt
    40741 gtcttgctgt ttccaatttt tcatgcgcgt ggcgaccaaa tccatgtatg cttgaaggat
    40801 ttccaaacgt gtcttgcatg attgccgtca tcgcttcaat tacttgtgga tgcatggggg
    40861 tagttgccgc atgatccaga taaatagttt tcacttcatt catcccttcc aaattattgc
    40921 taggcacatt ctagcataaa atagcgaaca aaccaaagaa gagctcattt ttgtctagag
    40981 gacgtttcat gggcatagaa aaaaggagct gtgacaaaag tcttgtcgca gctccttgaa
    41041 gctgctcact tctgtcacaa cttctt
//
LOCUS       DANJJH010000013        40414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000013 DANJJH010000000
VERSION     DANJJH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWS53_000667"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="PWS53_000667"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL0870935.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWS53_000668"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="PWS53_000668"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL0870936.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="PWS53_000669"
     CDS             complement(2303..2965)
                     /locus_tag="PWS53_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0870937.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="PWS53_000670"
     CDS             3149..4795
                     /locus_tag="PWS53_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0870938.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="PWS53_000671"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="PWS53_000671"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0870939.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="PWS53_000672"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="PWS53_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="PWS53_000673"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="PWS53_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="PWS53_000674"
     CDS             complement(8944..10023)
                     /locus_tag="PWS53_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0870940.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="PWS53_000675"
     CDS             complement(10126..10566)
                     /locus_tag="PWS53_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0870941.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="PWS53_000676"
     CDS             complement(10623..11033)
                     /locus_tag="PWS53_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0870942.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="PWS53_000677"
     CDS             complement(11213..11647)
                     /locus_tag="PWS53_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0870943.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="PWS53_000678"
     CDS             complement(11747..12595)
                     /locus_tag="PWS53_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0870944.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="PWS53_000679"
     CDS             12984..14750
                     /locus_tag="PWS53_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL0870945.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="PWS53_000680"
     CDS             14852..16111
                     /locus_tag="PWS53_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0870946.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="PWS53_000681"
     CDS             16258..17559
                     /locus_tag="PWS53_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870947.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="PWS53_000682"
     CDS             17562..18413
                     /locus_tag="PWS53_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0870948.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="PWS53_000683"
     CDS             18426..19241
                     /locus_tag="PWS53_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL0870949.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="PWS53_000684"
     CDS             19392..20558
                     /locus_tag="PWS53_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0870950.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="PWS53_000685"
     CDS             20788..21435
                     /locus_tag="PWS53_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0870951.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="PWS53_000686"
     CDS             21519..22463
                     /locus_tag="PWS53_000686"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL0870952.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="PWS53_000687"
     CDS             22598..24049
                     /locus_tag="PWS53_000687"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL0870953.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="PWS53_000688"
     CDS             24070..24906
                     /locus_tag="PWS53_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0870954.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="PWS53_000689"
     CDS             24906..25565
                     /locus_tag="PWS53_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0870955.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="PWS53_000690"
     CDS             25691..26380
                     /locus_tag="PWS53_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870956.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="PWS53_000691"
     CDS             26386..27342
                     /locus_tag="PWS53_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL0870957.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="PWS53_000692"
     CDS             27386..27898
                     /locus_tag="PWS53_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0870958.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="PWS53_000693"
     CDS             complement(28154..28702)
                     /locus_tag="PWS53_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0870959.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="PWS53_000694"
     CDS             complement(28791..29972)
                     /locus_tag="PWS53_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0870960.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="PWS53_000695"
     CDS             complement(30208..31716)
                     /locus_tag="PWS53_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL0870961.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="PWS53_000696"
     CDS             31875..32066
                     /locus_tag="PWS53_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870962.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="PWS53_000697"
     CDS             32269..33186
                     /locus_tag="PWS53_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL0870963.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="PWS53_000698"
     CDS             33179..34252
                     /locus_tag="PWS53_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870964.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="PWS53_000699"
     CDS             34400..34918
                     /locus_tag="PWS53_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870965.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="PWS53_000700"
     CDS             34899..35291
                     /locus_tag="PWS53_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL0870966.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="PWS53_000701"
     CDS             35367..36302
                     /locus_tag="PWS53_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL0870967.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="PWS53_000702"
     CDS             36435..37214
                     /locus_tag="PWS53_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0870968.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="PWS53_000703"
     CDS             37207..37524
                     /locus_tag="PWS53_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0870969.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="PWS53_000704"
     CDS             37768..39378
                     /locus_tag="PWS53_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0870970.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="PWS53_000705"
     CDS             39535..40383
                     /locus_tag="PWS53_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0870971.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DANJJH010000014        39223 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000014 DANJJH010000000
VERSION     DANJJH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            131..505
                     /locus_tag="PWS53_000706"
     CDS             131..505
                     /locus_tag="PWS53_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0870972.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWS53_000707"
     CDS             complement(619..1248)
                     /locus_tag="PWS53_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0870973.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWS53_000708"
     tRNA            complement(1382..1454)
                     /locus_tag="PWS53_000708"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWS53_000709"
     tRNA            1668..1741
                     /locus_tag="PWS53_000709"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWS53_000710"
     CDS             complement(1879..2043)
                     /locus_tag="PWS53_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0870974.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWS53_000711"
     CDS             2338..3666
                     /locus_tag="PWS53_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL0870975.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWS53_000712"
     CDS             3868..4701
                     /locus_tag="PWS53_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL0870976.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PWS53_000713"
     CDS             4717..5454
                     /locus_tag="PWS53_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL0870977.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PWS53_000714"
     CDS             complement(5609..6577)
                     /locus_tag="PWS53_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL0870978.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PWS53_000715"
     CDS             6809..7642
                     /locus_tag="PWS53_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL0870979.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PWS53_000716"
     CDS             7676..8515
                     /locus_tag="PWS53_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0870980.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PWS53_000717"
     CDS             8541..9101
                     /locus_tag="PWS53_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0870981.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PWS53_000718"
     CDS             9300..11021
                     /locus_tag="PWS53_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL0870982.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PWS53_000719"
     CDS             11186..12328
                     /locus_tag="PWS53_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0870983.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PWS53_000720"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PWS53_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL0870984.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="PWS53_000721"
     CDS             14128..14775
                     /locus_tag="PWS53_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0870985.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="PWS53_000722"
     CDS             15168..17813
                     /locus_tag="PWS53_000722"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL0870986.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="PWS53_000723"
     CDS             18123..19436
                     /locus_tag="PWS53_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL0870987.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="PWS53_000724"
     CDS             19426..20079
                     /locus_tag="PWS53_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0870988.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20685
                     /gene="radC"
                     /locus_tag="PWS53_000725"
                     /pseudo
     CDS             20134..20685
                     /gene="radC"
                     /locus_tag="PWS53_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            20816..21901
                     /locus_tag="PWS53_000726"
     CDS             20816..21901
                     /locus_tag="PWS53_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0870989.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            21898..23790
                     /locus_tag="PWS53_000727"
     CDS             21898..23790
                     /locus_tag="PWS53_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0870990.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            23797..24174
                     /locus_tag="PWS53_000728"
     CDS             23797..24174
                     /locus_tag="PWS53_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0870991.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            24328..25110
                     /locus_tag="PWS53_000729"
     CDS             24328..25110
                     /locus_tag="PWS53_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL0870992.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(25236..25967)
                     /gene="erm(A)"
                     /locus_tag="PWS53_000730"
     CDS             complement(25236..25967)
                     /gene="erm(A)"
                     /locus_tag="PWS53_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL0870993.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(26025..26084)
                     /locus_tag="PWS53_000731"
     CDS             complement(26025..26084)
                     /locus_tag="PWS53_000731"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL0870994.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            26476..27138
                     /locus_tag="PWS53_000732"
     CDS             26476..27138
                     /locus_tag="PWS53_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0870995.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            27373..27516
                     /locus_tag="PWS53_000733"
                     /pseudo
     CDS             27373..27516
                     /locus_tag="PWS53_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            27666..27866
                     /locus_tag="PWS53_000734"
     CDS             27666..27866
                     /locus_tag="PWS53_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0870996.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(28120..29799)
                     /locus_tag="PWS53_000735"
     CDS             complement(28120..29799)
                     /locus_tag="PWS53_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0870997.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(29801..30013)
                     /locus_tag="PWS53_000736"
     CDS             complement(29801..30013)
                     /locus_tag="PWS53_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0870998.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            30406..32136
                     /gene="efrA"
                     /locus_tag="PWS53_000737"
     CDS             30406..32136
                     /gene="efrA"
                     /locus_tag="PWS53_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL0870999.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            32133..33893
                     /gene="efrB"
                     /locus_tag="PWS53_000738"
     CDS             32133..33893
                     /gene="efrB"
                     /locus_tag="PWS53_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL0871000.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            33988..34185
                     /locus_tag="PWS53_000739"
     CDS             33988..34185
                     /locus_tag="PWS53_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871001.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            34338..34823
                     /locus_tag="PWS53_000740"
     CDS             34338..34823
                     /locus_tag="PWS53_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL0871002.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(34927..35520)
                     /gene="clpP"
                     /locus_tag="PWS53_000741"
     CDS             complement(34927..35520)
                     /gene="clpP"
                     /locus_tag="PWS53_000741"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL0871003.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            35700..36227
                     /locus_tag="PWS53_000742"
     CDS             35700..36227
                     /locus_tag="PWS53_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871004.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            36318..37055
                     /locus_tag="PWS53_000743"
     CDS             36318..37055
                     /locus_tag="PWS53_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0871005.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            37059..37976
                     /gene="miaA"
                     /locus_tag="PWS53_000744"
     CDS             37059..37976
                     /gene="miaA"
                     /locus_tag="PWS53_000744"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL0871006.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            37978..39207
                     /gene="hflX"
                     /locus_tag="PWS53_000745"
     CDS             37978..39207
                     /gene="hflX"
                     /locus_tag="PWS53_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL0871007.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        13178 a         6425 c         8149 g        11471 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttttaaccat agtataataa
    20701 ataatagtag ttaaagtagt ttaagaggag atattaaagg gatattttac tttaaataaa
    20761 aattaacaag gtttataatt tgtagtagtt aaagtattta aagaggtggg aacatgtgaa
    20821 ggttcaaagg atagaagtgg agaataagcc gtatccattg tatttattac tagataaaga
    20881 ataccagcta atagaaccag taatgaaatt tattaaatac ttagataata ctggtaagtc
    20941 tcctaatacc attaaagcat actgctatca tttaaagttg ctgtacgagt tcatggaaca
    21001 gagaggtgtt attcttaatg atattaactt tgagttgtta gcagacttcg taggttggtt
    21061 gagatatcct tcagcatcaa atgtaattga tcttcagtca aaaaaagcca taagagaaga
    21121 aacgacagtg aatacaattt taaatgtagt tatgagtttt cttgattatt taagtagatt
    21181 aggagaattt aaatcaattg atgtatttaa acaagcaaag ggaagaaatt tcaaaggatt
    21241 tttacatcat gttaataagg gtagatacca aaagaatgtc ttaaagttaa gggttaaaaa
    21301 gaaacagata agaacattga gatcagagga agttaagcaa attattgatg cttgtcatac
    21361 gaaaagagat aaattaattt taatgcttat gtatgagggt ggtttaagaa tcggtgaagt
    21421 gttatcgctt aggcttgaag atattgtcac ttgggacaat caaatccatt taacacctag
    21481 agatgttaat gttaatgaag cttatattaa attaaggaag gaaagaacaa tacatgtgag
    21541 taaagaactt atgtcacttt atacagatta cttgatatat gagtatagtg aggaattgga
    21601 gcatgattat gtttttattt ccttaaaaga aggctatttt gggaaaccac taaagtacca
    21661 aagtgttctt gatctagtta gaagaatagt taaaaggact ggaatagaat ttacatcaca
    21721 tatgcttcgc cacactcacg caacgcagct aattagggaa ggatgggatg ttgcgttcgt
    21781 tcaaaagaga ttaggtcacg cacatgttca gacaacgtta aatacctatg ttcatctttc
    21841 agatcaggat atgaaaaatg agtttaataa atacctcgag agaaaggagc ataagaaatg
    21901 aatgcttcta gtaaaaggaa aattattagt cagagtgaga ttagcaaaaa aatagctgta
    21961 atgaatgaag aaatgcaggg gttttgggct aataatagtt gggatataag aaaatgtcca
    22021 catccttctg ccatagaatt aagtaagaat cctgctttaa ggaatcgttg ggttcgtttt
    22081 gaacgtgtta aaaatctgtg gttaagaaca gaattgaaat atttttattt ttaccattta
    22141 aacaatggaa tatggaatgc aaaaactgtc tggattagaa aaggaacagt aattaataaa
    22201 atgttagatt tcttagattt aaagtatcct agcattactt caattactga agttcctatt
    22261 gaaaaagcaa tgacggagta tagaacttat ttgacaaaac ggggtgttag aattaccact
    22321 actaattata agattactgc taatcaagaa aaaacacctg taaaagctaa ttcctactat
    22381 gttactaatc taaaacaatt tatggagttt tatgagaact tttattttga tggagaggag
    22441 tgggataaag acgtttggga tagacgtaac ttacctttgc cagatgataa ggttaaccca
    22501 acacaatatg aatatacaat taactttaaa gggtttcgga atacatattt taaacaactt
    22561 gtaaaaagat attgtaagtt gagattgaac atggatagct tttcctatgt aagtgatatt
    22621 gcccaaagac ttaaagagtt ctttaatttt ctggacatga aatttaaaca agttcagaga
    22681 gtacaccaat taacgagagt ggaaattgaa gcatatttaa gtgaactaaa catgatggga
    22741 ataaaaccta gtacaataac tgggaggatc tctatattgg aaggactatt tagtaccctt
    22801 cttaggctag aatgggatga tgttccttcc aaaatattaa tttattctga ggactatccg
    22861 aaaataccaa gagcaaaacc acgctttata gatgaattcg tcctagagca attgaacagt
    22921 catcttgata aattacccga atatatagct acgatgacta tgattgttca agaatgtgga
    22981 atgaggataa gtgaattgtg caccttgaaa aaaggctgtc tattagagga caaagatgga
    23041 gatttctttt taaagtatta tcaatggaaa atgaaaaagg agcatatagt tccaatatct
    23101 aaagaggtag ctttacttat taaagttcgg gaagataaag tttcagagga atttccagat
    23161 agtgaatacc tctttccaag aaaagatgga tcgccattaa aacaagaaac atttagaggt
    23221 gagttaaata aattagctta tgagcaaaat atagtggata aatcaggtga gatttataga
    23281 ttccatgccc atgcctttcg ccatacagta ggaacaagaa tgattaacaa cgggatgccc
    23341 cagcatattg tgcagaaatt tttggggcat gaaagcccag aaatgacaag cagatacgct
    23401 catatctttg atgaaactct aaaaaatgaa tttactaaat ttcaggaaaa actggttacc
    23461 aataatggag atgtgcttga tctagatgaa gataatgaag tcgatgatgt agagcttcaa
    23521 tggttcaaga aaaatataaa tgcacaagtg cttccaaatg gttattgtag attgccagta
    23581 gtagcaggtg gttgtccaca tgcgaatgca tgcttagatt gcactcactt ctgtaccagt
    23641 aagcaattct taccacagca cgaagaacag ttagagcgta cagaagagtt attagccata
    23701 gctaaggata aacaatggca aagacaagta gagactaata gccgtgttaa agagcgttta
    23761 gaacaaatca ttggaagttt gacggggtaa ttatcaatgg ataaacaagt tagaaataca
    23821 acagaaattg tacgtttggc gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa
    23881 gcgatttcta aattttcgat tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa
    23941 gctaatgttt ctaaatcatg gctttataag gaacacgata ttaggcaaag aatcgaatcc
    24001 cttcgtgagc gtcaaataac agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg
    24061 gaggaaatcc ttattaaaac cttaaaaaga agagtaatgg aattagaaaa agaaaataaa
    24121 aaattacaga accaaattca aaaattatat ggagatctgt ataataaaga ataattatta
    24181 atctgtagac aaattgtgaa aggatgtact taaacgctaa cggtcagctt tattgaacag
    24241 tcatttaggt atatgtccaa tctagggtaa gtaaattgag tatcaatata aactttatat
    24301 gaacataatc agttacgagg tgaaatcatg agcaatttga ttaacggaaa aataccaaat
    24361 caagcgattc aaacattaaa aatcgtaaaa gatttatttg gaagttcaat agttggagta
    24421 tatctatttg gttcagcagt aaatggtggt ttacgcatta acagcgatgt agatgttcta
    24481 gtcgtcgtga atcatagttt acctcaatta actcgaaaaa aactaacaga aagactaatg
    24541 actatatcag gaaagattgg aaatacggat tctgttagac cacttgaagt tacggttata
    24601 aataggagtg aagttgtccc ttggcaatat cctccaaaaa gagaatttat atacggtgag
    24661 tggctcaggg gtgaatttga gaatggacaa attcaggaac caagctatga tcctgatttg
    24721 gctattgttt tagcacaagc aagaaagaat agtatttctc tatttggtcc tgattcttca
    24781 agtatacttg tctccgtacc tttgacagat attcgaagag caattaagga ttctttgcca
    24841 gaactaattg aggggataaa aggtgatgag cgtaatgtaa ttttaaccct agctcgaatg
    24901 tggcaaacag tgactactgg tgaaattacc tcgaaagatg tcgctgcaga atgggctata
    24961 cctcttttac ctaaagagca tgtaacttta ctggatatag ctagaaaagg ctatcgggga
    25021 gagtgtgatg ataagtggga aggactatat tcaaaggtga aagcactcgt taagtatatg
    25081 aaaaattcta tagaaacttc tctcaattag gctaatttta ttgcaataac aggtgcttac
    25141 ttttaaaact actgatttat tgataaatat tgaacaattt ttgggaagaa taaagcgtcc
    25201 tcttgtgaaa ttagagaacg ctttattact ttaatttagt gaaacaattt gtaactattg
    25261 aaaatagaaa gaaattgttc cttcgatagt ttattaatat tagtgacatt tgcatgcttc
    25321 aaagcctgtc ggaattggtt tttagtgaaa agaacacgat attcacggtt tacccactta
    25381 taaacaaaag atcgatactt tttgtagtcc ttctttgaaa tcaatggttg atgtcgttca
    25441 agaacaatca atacagagtc tacacttggc ttaggatgaa aatatagtgg tggtactttt
    25501 ttgagcattt ttatatccat ctccaccatt aatagtaaac ccaaagctcg ttgcagattt
    25561 tgcaatcttt tcgcaaatcc cttctcaacg ataagatagc tatatttagc ctgactttca
    25621 aaggtaattc ttttgacaat atccgtactg atgttataag gaatattacc atatatctta
    25681 tagtttatat gttttgggaa ggaaaatttt agaatatccg tttgaatcac ttttatattc
    25741 tcagaggggt ttaccgcttc tttagtcact tgacataagc ctccatcaat ttctatagca
    25801 gtaactgatc gactcatttt gactagctct ttggtaaaat gtccttttcc tgatccgatt
    25861 tctattacgt tgtcttgttt actgatattc gtgtgattca atatttcttt tacatgcttt
    25921 ttagaagtaa taaaattttg cgtgtcttta gggtttttct ggttcattat aaccttctcc
    25981 ttactggtta taatgaactg acattaagcg ttcattataa ccaattaatt ttgatttggt
    26041 tgataacgaa ctttattaat aacaaatata gaaaaagtac ccatactttt atctcctttc
    26101 catttttttc attatgaatg agataaagta atttctacta ctgcgatact ggtgcacata
    26161 attgaaacct ccttatgtaa ctgaatatga ttgtatcatc ttttcatttt agaataaacc
    26221 tttatatgta attttgcact tgacacttca gatggacgtt ttcttcaaaa taatatgttt
    26281 tgaaacttga taagagaagc cactgtcttt actaaaaagt aaaggctgcg ataaaggaca
    26341 gtgactttaa taaagttgca ctgttgtatt gtctttggat aaaattgtct aaagggaagt
    26401 tttatcccca acgaaaaaac atctaaagtg gtaagtaatg aaagaatgtc gataaagtag
    26461 gaatggggga tctttttgaa tacaatagaa tcacagtttg ataaggttgc agaagattac
    26521 gatttcgtga atgagctttt gaatgattat tcattctttg tgtctaatat gtctccaaag
    26581 aaaggcagag cattagatat cggatgtggc tcgggtttgt tagtggagaa attagcaagt
    26641 tattatgatg aagtggtagg gattgatatt tctaatcaaa tgctcgatct tgccaaatct
    26701 aaacgtcaac taacaaatac ggtctatctg aatatgaatg cagaacaact taattttaat
    26761 gagaagtttg attttattgt aagccgaaca acctttcatc atttggatga tatagccagc
    26821 gtaatacagc aaatgaagga actgttgaat gaggaaggaa gaatagttat tcttgataat
    26881 gtatctgaag tcgagacacc gcctacctat gtttataaat taggggcaat ccaagagttt
    26941 ttaccgcatt gtttcaaatt tggaataaaa aatgcgatta gaatttataa tcacaacaca
    27001 tctaaatcat ggcttgagca tttagcttca gataagtacc tatctgaaca aaattattac
    27061 gatttatatg aaaagttgtt gcctggatgc caattccata aaatgggttg ggcaatgggg
    27121 gttgtctgga caaaataact taaagctgtg gctttgaaaa gttgaatttg tgattgtttt
    27181 cagagctatc gggtgcttta gtataaataa caatgaataa aaaatcagcc tgtatcttat
    27241 gttactatta agtaatttaa gatacaggtt ttactatttt tgaaataagt accttccact
    27301 tcaaatacat gtattcaaag tgaaagtatt gatttaaatg attcttttgt aatttactct
    27361 tgataatatg aaattccatc gcagcaggat atgaatttta ccaaaaggat acaaaagtgt
    27421 ggagaaatga tggggatcac tgtactggac cacttgatca tcggacgcaa acgatatttt
    27481 agtttgagag aagaaggaat gatggaagag aagtaagttg ctttcttgca aaaaaaatga
    27541 agcaagtgta caattatatt gtaattttgt ttgggaatag aagttgaaac atctgttgca
    27601 cctctatcgt acacgagcaa tgttacaatt taaattagtg cttttggcac gaggaggata
    27661 ttattatgga aaacggaaca gtaaaatggt tcaacgctga aaaaggattt ggatttatct
    27721 cacgtgaaga tggaagcgac gtattcgttc acttctcagc tatccaaggt gacggattca
    27781 aaacattaga agaaggtcaa gcagtgactt tcgacgttga agaatcagac cgcggcccac
    27841 aagctgcaaa cgttgtaaaa gcttaatttt atctgtaaat caaattgatg gtagacatca
    27901 atttttacga gagaacaaag cgagtaacaa aaatacctgc cacaagagac aatttctctt
    27961 gtggcaggta tttttttgta tgaaaaagta aataaattta agaggctatg aaagagccac
    28021 cttgtatcaa ggaataagaa caaacaaaaa tggcttctca tgtttttcat ttgttttctc
    28081 ttatattcag gacaaggtgc tgatttcata acctcttatt tattcttcat ctggtgtcat
    28141 taccattggc aaaatcattg gatgtctttc tgtacgttcg aataagaaag gctgaagcgc
    28201 gccagtcatc gcttctttca atttatattc tgaacagtca ggtccattca aacattctct
    28261 taaagcgtgg aataagattc gctgagcttc attgatcatt tctcctgatt ctcgcatgta
    28321 gacaaatcca cgagagagaa tatctggacc agccaagatt tcttttttct tgatatccac
    28381 tgtcgcgaca gctaatacaa gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac
    28441 attaccaata tcaccgatcc cgctaccatc gatatacaca tctccagcca caaaatgccc
    28501 agcaggacgc gcagaatcgc tagttaacgc taaaacatcg ccattttcca tgatgaagca
    28561 gttttctgca ggaacaccta catcttgtgc tagagaagca tgtactttca acatgcggaa
    28621 ttctccgtgg acaggaataa aatattttgg cttcatcaaa cgaagcatta atttttgttc
    28681 ttcttgtcca ccgtgaccgg aagtatggat gttattgatt tttccgtgaa tcacttctgc
    28741 accagcttca gacagcaaat tgatcaaatg attgacactt gttgtattac caggaatagg
    28801 tgaacttgaa aagatcactg tatcgtctgg ctggatttgg atttgacggt gagtaccatt
    28861 cgcgatgcgg ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata ggatcatcgt
    28921 ttcattggca ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt
    28981 gatatatcct aaacgttgac cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc
    29041 gattttacga ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga agatgttaga
    29101 agcaaagctg gcaaaaatga tacgtccttc gatttttgaa aagatcttca aaatagaatg
    29161 cccaatcgtt ttttctgatt tagtaaatgt tggaatctca gcatttgtac tatcagataa
    29221 aaggcacaag actccttctt ccccgatacg agccatttta tgcaagtttg ctggttcgcc
    29281 aacaggagtg aaatcgaatt tgaagtctcc agtcgctacg atatttccag aaggtgtttt
    29341 caccacgatc cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt
    29401 ctttctaaaa cggatgatcg tatcttcatt gatctcatgg agctctgcat ctcgaagcaa
    29461 gccatgctca tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag catagatagg
    29521 aatatttgct tgtttcagca agtagggtat tccgccgatg tggtcttcgt gcccgtgagt
    29581 aatcaccaag gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac
    29641 atagtcaatt ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat
    29701 ttcatcttgg aattgtaccc catacgtatt tttcccgatt tcacctaatc caccaatagc
    29761 aaagacgcct gtctcgttat ttttgatgga aagtttcata ttaaaactcc actaatgaga
    29821 aatcagcatt ttctttttcg tactctagat gacttccttc tagaagttga acgaattcaa
    29881 tattgtatgg ggtattttct tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa
    29941 tgtaaagaga ttttgtttct tcccgtttag ggttgcgtac ttttgtttct tgataataaa
    30001 ctttatagat cattgttttg tctcctttat ctgcgtaaag cagtgattta gctagttgat
    30061 ttttttagac aacacaaaac gaggggacaa gccacctggt ttttcatatg ctttgactag
    30121 aatactggat tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc ggatattttt
    30181 tcaaccgtac cttctgtata aaaaatatac aacaattaat tgttattcta gcataaaaca
    30241 gcaaataagt atagaaatca actagattga aagaaaaaat caatgtttat catcccgcta
    30301 taatcataat gactttttta tgagagaagt gttctattaa taggctcgtt gtgctatcat
    30361 ttttagtaat gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa actaatgtgg
    30421 cattatacca tgcgttataa gaaattcttg tttttcaatt tcatatgcgt atttggtttt
    30481 attttgattg aattaggact tcctacgatt ttagcacgta tgatcgatgt aggaatccgc
    30541 aatgatgatt ttgattatgt gaagcaacaa gggatattta tgatcgttat tactgtaatc
    30601 ggtattattt tgaatatctt gttaggttat tttggttcga ggattacgac taatatcgta
    30661 gcagatattc gagatgattt gttcgaaaag atccaaagtt attcgcatca ggaatacgaa
    30721 aatctaggcg tatcttcatt gatcactcgt acaacaaacg atgcttatca gatcatgttg
    30781 ttcctgcaaa atattttacg tatcggtttt atggcaccaa tgatgttcat tgtcagtttg
    30841 tacatggtca tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc attaccttta
    30901 ctattattgg cagtcgtagc gattgctaag gtgtctgaac cgttatctaa aaagcaacaa
    30961 aaaaacttag ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt
    31021 cgtgcattcg tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat
    31081 gcttctagtt caaaaagctt attccgtttg atggctgttg cacaaccggg tttttatttc
    31141 ttatttaata tcgtaatggt attgatcatc tggagcgggg cagtgcaaat cgatgcaggc
    31201 gagcttcgtg taggagattt gattgcgttt atcgagtata tattccatgc cttgttctca
    31261 ttcatgttat ttgctagtgt gttcatgatg tatccaagag cagctgtttc agctggacgt
    31321 atccaagaag ccctagatgc caaaccttcg attgtagaag atccaaatgg tatatctgat
    31381 acaaaaacta aagggtatat cgaatttcaa aatgtaacat ttgcttatcc aggccattcg
    31441 caagagccag tgatacgaaa tgtcagtttt acagcctctc caggagagac cgttgctttt
    31501 attggaagta ccggtagcgg aaaatctacg ttgatccaat tgatccctcg cttttatgat
    31561 gtgagtgaag gaaagatctt gatcgatgga gtagacgtga gagattatcg gctaagcaag
    31621 ttgcgagata agattgggtt tattccgcaa aaagcgctgc tatttactgg aacgattgca
    31681 gataatttgc gatacgggaa agaagatgct acgcaagaag aaatggaacg tgcagctgag
    31741 attgctcaag cttcagaatt catttctcga aaaccagatg gatttaatga acatttatca
    31801 gaaggtggtt ccaatttttc aggtggacaa aaacaacgat tggcaatagc cagagccatc
    31861 atacgccgtc cggaaatcta tatttttgat gacagctttt ctgcattaga ttatcaaacg
    31921 gatgcgaagt tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt attgatcgta
    31981 gcacaacgtg tgggaacgat catgcatgca gataagatca tcgtattaaa tgaaggagaa
    32041 gtcgtcggta tggggacgca tcgggagttg ttggaaaaat gcccgattta ttatgatatt
    32101 gccgcttctc aattatcaaa ggaggaatta gcatgaaaaa aaccttttct tccttaaaac
    32161 gtttagctcg atatatcaga ccgtatcgtg agacttttct tttagtgatc ctatttacag
    32221 ttctgacagt ggctttcaac gtggctttgc catatgttac aggacttcct actacagaaa
    32281 tcagccgtaa tattgccaat ggcgaatcaa tcaatttttc ttatatattc aaatgtctca
    32341 tttggatcac agttgttgga atagggtatt gtatttcgca attactttct ggtgttttga
    32401 tgacaaatgt cgttcaaagt gccatgcatg atttgagaag agacatagaa gaaaaaatca
    32461 atcgtcttcc ggtttcatat ttcgataaaa accagcaagg aaatattttg tctcgtgtga
    32521 cgaatgatgt agatgcagtc agcggtgctt tacaacaagc ttttattggt atcgtaaatg
    32581 cgatattggg aattacgttg gctgcagcaa tgatgtttta tatccagccg atgatggcgc
    32641 tgatctctat gatcatgatt ccaacatcga tctggatctc aaaacgagtg atcaatgctt
    32701 cgcagaaata ttttcaaagc atgcagaatt ctcttggtga gctaaacggc tacgtccaag
    32761 aaaatatgac aggttttagt gtattgaaag tatatggacg cgaaaaagaa acattggaag
    32821 gattcaaaaa agttaatcac agcttgaaat actttgggtt tcgtgcagca ttcatttctg
    32881 gcttgatgat gcctctagtt caattaactg cctatgctac ttatattggt atggctgttc
    32941 taggaagctt ttatgttatt actggggcga tcgttgtcgg tcaactacaa gcattcatcc
    33001 aatatatttg gcaaatcagt cagccaatgg ggaacgtgac acaattgtct tcagttttgc
    33061 aaagtgcatc agcggcaacg aaacgtgtat tcgagatttt agatgagcca gaagaaaaag
    33121 aaaacaaagt agatgcttca ctgccagaaa cgattttagg agatgtgaca ttcgatcatg
    33181 tcgattttgc atatgatccg aagaaaccat taatccaaga tttgaatttt gaagtgaaag
    33241 ctggtcaaac cgtagctgtg gtcggtccga ccggtgccgg caagacgaca ttgatcaatc
    33301 tattgatgcg tttttatgac gtagacaaag gagcaataag gattgatgga atcgacacca
    33361 aatcaatgag tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc
    33421 tttatcaagg aacgattgca gataatatcc gcttcggaaa gcttgatgcg acagattatg
    33481 aagtagttga tgctgcgaaa acagcaaacg tagatcattt tatccgaacg atgccagatg
    33541 gatatgatat ggccatcaat tctgagggag acaatgtgtc tttaggacaa aaacagttgc
    33601 tgacgattgc ccgagcagtg gtctccgatc ctaaaatctt gattttagat gaagcaacaa
    33661 gttcagtaga cacccgttta gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg
    33721 gacggacaag tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca gatctgattc
    33781 tagtgatgga tcaaggtcag atcattgaaa aaggaacgca tgagagttta ttggcacaag
    33841 gtggatttta tgaaaaactg tataacagcc aatttgctga ggaagcagaa taagtcatat
    33901 aagaataaaa cagtttaaaa cttatttttt gttctctatt cttgataatg gtaaagtaaa
    33961 gttatcaaga aaagtgagga cacggagatg gaacagtttt taaatgaaaa aattactgaa
    34021 gccaaaagag aagttgatcg tttaacgcac gtctgtcaga cgaagccttt tgattcagct
    34081 gatttttttg aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga agtgtacgaa
    34141 ttaattttaa aaaaagtaat agaagaaaat atagatgtac aataaaaaca ggtaaaacgt
    34201 cagttaccag atactgtaaa tagacatctg gcctgagcag gttttacctg ttttttttct
    34261 tatgaaataa atatatgcta tgataccttt attcaatagg attagtttct acaaaaaatg
    34321 aaaggagaaa aataaatatg gaacggttta tagcatcaaa tgccacagtg attggtgatg
    34381 tgacattgtc agaagatgtg acgatctggt atcaagctgt tttacgcggg gacagtaatt
    34441 ggataaaaat aggacaaaga acgaatatcc aagatggtac aatcatccat gtcgatcatg
    34501 atgcacctgt agatattgcg gagaatgtga cggtaggaca tcagtgcatg ctacatggct
    34561 gtacaatcga aaaaggggcg ctgatcggga tggggaccac gatattgaat catgcggtta
    34621 ttggtgagaa tagtttgatt ggtgctggat cattagtaac agaaggaaaa gttattcctc
    34681 ctaatgtctt ggcttttggt cgcccagcta aagtgatccg tcctttgaca aaagaagaaa
    34741 tacaaaaaaa caaagaaaac atccagcatt atgtcgagat aggtcggatg catgcgcaag
    34801 gaaaattcaa ggagcaaaga taggcagtag catccataaa ataaaaaagg atggtcagtt
    34861 tgagttgacc atcctttttt atttggttta gataagagag cttaaaaaag cttcctttga
    34921 aacagattaa tttaatgaag aactgttttc catgacttca tcgatcaatc catatgcttt
    34981 tgcttgttca gctgtcatat agttgtcacg atctgtatct ttttcaatca cttcaagcgg
    35041 ttgtccagtg cgttcagcta aaattttgtt taagcgttcg cgtgtttgca aaatatggcg
    35101 tgccgcaatt tcgatttcag ttgcttgccc ttgagcgccg ccaagaggtt gatggatcat
    35161 gatctcagca tttggtaaag caaatcgttt accttttgta ccagctgtca gcaagaagct
    35221 tcccattgaa gcagccatac ccataacaat tgtttggaca tctgctttga caaagttcat
    35281 tgtgtcgtaa atagccatac ctgctgtaac tgaaccacca ggagaattga tataaagata
    35341 gatatctttt tcagaatctt gagcgtctaa gaaaagcaat tgggcgatga tcgaattagc
    35401 aagatcatct gttacttggc cgcttaacat gatgatacgg tctttcaata aacgtgagta
    35461 gatatcgtaa gctctttcac ctcgtgaaga ttgttcaatg actgtaggaa ttaaattcat
    35521 aatagagttc ctccttgatt tcgtttagaa atagtgtacc ataatttagt ttctaatacc
    35581 taaaatgtag tatacatttt tggtcaaaaa aggtcaaata aaaaacttag gcaaattagt
    35641 cgcataaacc cgctttatgg tatgataagc agagaaacaa aacaaggagg aacaacagat
    35701 tgatagttgg tattactggc cttatcggag tgctgacagg tgctgtagtt gtctatatcg
    35761 gcatgatact gcaaatgcgt cacttggaaa ataaagaaca gcgtagacgt gaattggaat
    35821 tgaaagtcaa agaaatcgag acgctgaatc aattaaataa aaagatcaac gagatcttgc
    35881 aaaaacggac aacgctgatg gaagaatatg tcagctttga tgcatttgat gattgttata
    35941 tcacgattga cgattttgcg tatctccaat cattttcagc gcaaaatagt ttttatctgc
    36001 caaattatat tttagacgag ctatttaaga atatctccca tcgaaaagtg attttatctc
    36061 cagatgaaac aatgaagatt ggtggatatg cttataaagg cggacgcgtg attttagaga
    36121 attttacgga tagtctgact gaaatgatca atgaaaaaaa agctcagttg aagactatga
    36181 cgcgccagcc gattggtttc ttctcgaaag atgatgtttg gagctgatat tttgaagcat
    36241 tgccgttgca catgagagcg tggtaatgct ttcttttttt cttaatgata caatagaact
    36301 gaacggagtg aagaaagatg ttacaaatct atgcccatcg cggaagcagc ggcacccacc
    36361 cagaaaatac gcttcctgca tttgcagaag ctgtacgagt aggtgcagat ggtatcgaac
    36421 tagatgtcca tctaagcaaa gatggttatt tgatcgttat gcacgacgaa gaagtggacc
    36481 ggacgactaa cggtaaaggg ctgattcgag aaaaaacatt ggaagaactc aaaaaattga
    36541 atgcaggggg caaattcagc aaagaatttc cagcagctaa agttcctgaa ttgagagaag
    36601 tgttaggact gctcctccaa aaaaattacc gagggatgct aatgattgaa attaaaactg
    36661 atcagtatga atatcccggt atcgaagaaa aattagccga aacattaaaa agtagagatt
    36721 ggccttttca acatgcatat tgcagctttc atttggattc tttggaaaaa atcgcattac
    36781 tggaaccgga gatacagctg gacttattga tgagtacttc tgataaaaag atccaactag
    36841 cagcagaaac cccttatata gaaggagtac atccaaaaaa tacttggttg ttcgaacatc
    36901 cagtccaggc agctgatttt ccaaaagcaa ttcgtgcatg gacgattaac gatgaagaag
    36961 aaatggccaa atgtcaagaa ctcaaaatag atggaataat aactgatttt ccggagaaag
    37021 ctcttcggtg gaaacaatca acgaaaagta ggtaaaatat ggaaaaagta ttagtgatcg
    37081 tcggtccgac agcagtcggt aaaacagcgc taagtgtgga actcgcacaa ctatttggag
    37141 gagagatcat cagtggtgat tcattgcaaa tctataaaaa tctggatatc ggtacagcaa
    37201 aaatcaaaga agaagagatg aataatgttc cgcatcattt gattgatgtg atcgaaccta
    37261 ctcaacgtta ctcagccgct gattttcagc attcaggtcg acaattgatt accgagattg
    37321 cgaaccgagg gcacctacct atcatagctg gtggtacggg tctctatatc caatcccttc
    37381 tatatgatta tcagttagga gcgacagaag aagcgaatcc tgagattaga aaaaagtatg
    37441 aaacgttagc tgaaacaatt ggttcccaag cattatggaa tcagttgaaa gaaaaagacc
    37501 cggcagctgc tgagaaaatc cactggaaca atcaacgcaa agtgataaga gctctggaag
    37561 ttttagagac gacaggacat agtatcacgg cacctaaaga agagccaaag caattgtatg
    37621 attacctcat gattggatta gatacgaagc gtagtattct ttatgaacga atcaatcaaa
    37681 gagtcgattt gatgctagca gaaggtttag tagcagaagc gcgtaaggtg tatgagcttg
    37741 gagaagtcca ggccagtcaa ggtattggat ataaagaatt tttcccttat tttgaaggga
    37801 aagaaacatt ggaagaagca acagaacaag tcaaactaca ttcgagaaga tacgcgaaac
    37861 gtcagttgac gtggtttcgt aaccgtttgg atgcacagtg gtttgatctt ataagtgagc
    37921 caaatcaaaa aagtcagatt gagcaaacaa tcaaaaaatg gctggaggcg gaataaaatg
    37981 gaagaaaaag tgatcatcgt aggggtagaa accgaagcaa accagcgtta cttttcagag
    38041 tcaatggaag agctagttca gctgacgaat accgcatcag gagaagtcgt attcacaatt
    38101 acacaaaaga gaccacaagt ggatcggcaa acgattatcg gtaaagggaa attacaagaa
    38161 ttagttcaac aagcggatgc gcatgaagcg gatttgatca tctttaatca tgagctaact
    38221 ccgagacaga gccaactgat taccgatgct gttggtcttc cggtaattga tcgcgtgcag
    38281 cttatcttag atatttttgc gatgcgtgct cgttcgaaag aagggaagct gcaggttgaa
    38341 cttgctcagt tagaatacct gctgcctcgt ttagtagggc aagggaaaac attatctcgt
    38401 cttggcggtg ggatcggaac aagagggcca ggtgaaacaa agttagaaac agaccgccga
    38461 catatacgga ataaaatttt tgctgttaag aaagaattga aagaagtaga agctcatcga
    38521 gagcgcaatc gccaaaaaag aaaaaatagc gagatttttc agattggact tattggctac
    38581 acgaatgccg ggaagtcaac tatcttgaat ctattgacac aagcggatac ttattcgaaa
    38641 gatcaattgt ttgcaacgtt agatccattg actaagagat ggcgttttgc tgaaggattt
    38701 gagattactg taacggatac cgtcgggttc atccaagacc tgcctacgca actgatcgat
    38761 gctttccatt ctacattaga agaaagtcaa aatatggatc ttttgctaca tgtagtcgat
    38821 gctagttctc cagatcgtat tttacaggag cagactgtct taaaattgat ggatgaactg
    38881 aatatgaaag aaatgccgat actgactgtt tacaataagg cagaccagat cgacccagcg
    38941 atgttcacgc ctacgctatt cccaaatgta ttgatttcta ctcagacgaa agaaggtaag
    39001 atggcgttga tcgaagcaat caaacgtcag ctgatggaac taatgacccc ttatacactt
    39061 tctgtgccaa gcaacgaagg acagaaatta agcgagttga gaagacaaac aatggttttg
    39121 gacgaagcgt acatggaaga aacaaacgaa tatgaagtta aagggtttgc aaaaaccaat
    39181 tcaaaatggt taagaaaaaa ccaataaaat tttatttttt taa
//
LOCUS       DANJJH010000015        38902 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000015 DANJJH010000000
VERSION     DANJJH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(59..187)
                     /locus_tag="PWS53_000746"
     CDS             complement(59..187)
                     /locus_tag="PWS53_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0871008.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWS53_000747"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="PWS53_000747"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0871009.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="PWS53_000748"
     CDS             complement(1674..2729)
                     /locus_tag="PWS53_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0871010.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWS53_000749"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="PWS53_000749"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL0871011.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWS53_000750"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="PWS53_000750"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL0871012.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWS53_000751"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="PWS53_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL0871013.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWS53_000752"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="PWS53_000752"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL0871014.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWS53_000753"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="PWS53_000753"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL0871015.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="PWS53_000754"
     CDS             10491..12050
                     /locus_tag="PWS53_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL0871016.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="PWS53_000755"
     CDS             complement(12161..14092)
                     /locus_tag="PWS53_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871017.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWS53_000756"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="PWS53_000756"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0871018.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="PWS53_000757"
     CDS             complement(15021..15773)
                     /locus_tag="PWS53_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0871019.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="PWS53_000758"
     CDS             complement(15932..16141)
                     /locus_tag="PWS53_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871020.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="PWS53_000759"
     CDS             complement(16152..17480)
                     /locus_tag="PWS53_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871021.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="PWS53_000760"
     CDS             17616..17771
                     /locus_tag="PWS53_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871022.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="PWS53_000761"
     CDS             complement(17845..18432)
                     /locus_tag="PWS53_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL0871023.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWS53_000762"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="PWS53_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL0871024.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="PWS53_000763"
     CDS             complement(20012..21301)
                     /locus_tag="PWS53_000763"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL0871025.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="PWS53_000764"
     CDS             complement(21455..22387)
                     /locus_tag="PWS53_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871026.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="PWS53_000765"
     CDS             complement(22544..23203)
                     /locus_tag="PWS53_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0871027.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWS53_000766"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="PWS53_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL0871028.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="PWS53_000767"
     CDS             complement(24244..24534)
                     /locus_tag="PWS53_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871029.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="PWS53_000768"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="PWS53_000768"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL0871030.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="PWS53_000769"
     CDS             complement(26820..28175)
                     /locus_tag="PWS53_000769"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL0871031.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="PWS53_000770"
     CDS             complement(28199..29368)
                     /locus_tag="PWS53_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871032.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="PWS53_000771"
     CDS             complement(29365..30252)
                     /locus_tag="PWS53_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL0871033.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWS53_000772"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="PWS53_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL0871034.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="PWS53_000773"
     CDS             34332..34853
                     /locus_tag="PWS53_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0871035.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35552..36109)
                     /locus_tag="PWS53_000774"
     CDS             complement(35552..36109)
                     /locus_tag="PWS53_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871036.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36288..36899)
                     /locus_tag="PWS53_000775"
     CDS             complement(36288..36899)
                     /locus_tag="PWS53_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871037.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37012..37269)
                     /locus_tag="PWS53_000776"
     CDS             complement(37012..37269)
                     /locus_tag="PWS53_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871038.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37866..38144)
                     /locus_tag="PWS53_000777"
     CDS             complement(37866..38144)
                     /locus_tag="PWS53_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871039.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38178..38429)
                     /locus_tag="PWS53_000778"
     CDS             complement(38178..38429)
                     /locus_tag="PWS53_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871040.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10627 a         8397 c         6824 g        13054 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
    34981 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
    35041 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
    35101 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
    35161 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
    35221 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
    35281 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
    35341 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
    35401 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
    35461 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
    35521 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
    35581 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
    35641 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
    35701 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
    35761 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
    35821 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
    35881 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
    35941 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
    36001 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
    36061 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat
    36121 cacctctaca ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg
    36181 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
    36241 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
    36301 ttgttaacaa tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa
    36361 acttcttgcg cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata
    36421 aagtctacct ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat
    36481 gacattgatt gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt
    36541 cctagatcgt ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt
    36601 cgttctcttt tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta
    36661 tcttcaatta tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca
    36721 taggtgattt ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag
    36781 cttggcaaca tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata
    36841 atttgtacaa ttctcattga tccattgtat tccattttat tccccctatt actattcata
    36901 tccgcctatc tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt
    36961 actgagttac ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac
    37021 atgattaatt ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc
    37081 gacaacataa gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa
    37141 atttactgtc gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc
    37201 ttcagcacca ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac
    37261 agccctcacc gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca
    37321 aaggatacaa tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa
    37381 taggcattaa ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat
    37441 ctcatacatt attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt
    37501 ttgtaattgg tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc
    37561 gtctatttgt gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac
    37621 tattttgctc caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta
    37681 attgatatgc tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct
    37741 tcttgggttt tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga
    37801 aatgccgttt ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac
    37861 tttgattaat ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa
    37921 tgttcgaact ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat
    37981 gttatcaaag ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt
    38041 ttctgcaggt aatctctcct aatggcagct atttttacct catcagtgct gataaaccat
    38101 tgatcatcca catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa
    38161 aagtttaagg ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg
    38221 agaattgaga acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg
    38281 tctctccatc cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa
    38341 aggaataatc cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat
    38401 gaccatttat ttataggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg
    38461 aatgaattca aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat
    38521 actttaaaaa agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt
    38581 agcaaataac ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag
    38641 ccaggataga cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag
    38701 ccttggaata cagtagctct taattggtaa tactttttcc attataaata acctcttttc
    38761 attttatatt tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc
    38821 ctcgctgtgc taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg
    38881 ccagaataaa ccaattattt gt
//
LOCUS       DANJJH010000016        37438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000016 DANJJH010000000
VERSION     DANJJH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            38..2203
                     /gene="acm"
                     /locus_tag="PWS53_000779"
     CDS             38..2203
                     /gene="acm"
                     /locus_tag="PWS53_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL0871041.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2475..2960)
                     /locus_tag="PWS53_000780"
     CDS             complement(2475..2960)
                     /locus_tag="PWS53_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL0871042.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3050..3395
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3440..4081
                     /locus_tag="PWS53_000781"
     CDS             3440..4081
                     /locus_tag="PWS53_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0871043.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4100..4735
                     /locus_tag="PWS53_000782"
     CDS             4100..4735
                     /locus_tag="PWS53_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0871044.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4753..5568
                     /locus_tag="PWS53_000783"
     CDS             4753..5568
                     /locus_tag="PWS53_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871045.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5887..7323
                     /locus_tag="PWS53_000784"
     CDS             5887..7323
                     /locus_tag="PWS53_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0871046.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7512..9959
                     /locus_tag="PWS53_000785"
     CDS             7512..9959
                     /locus_tag="PWS53_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0871047.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10189..11256
                     /gene="rlmN"
                     /locus_tag="PWS53_000786"
     CDS             10189..11256
                     /gene="rlmN"
                     /locus_tag="PWS53_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL0871048.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11449..12552)
                     /locus_tag="PWS53_000787"
     CDS             complement(11449..12552)
                     /locus_tag="PWS53_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0871049.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13105..13653
                     /gene="aac(6')"
                     /locus_tag="PWS53_000788"
     CDS             13105..13653
                     /gene="aac(6')"
                     /locus_tag="PWS53_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0871050.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14199..15860
                     /locus_tag="PWS53_000789"
     CDS             14199..15860
                     /locus_tag="PWS53_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871051.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(15930..16277)
                     /locus_tag="PWS53_000790"
     CDS             complement(15930..16277)
                     /locus_tag="PWS53_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL0871052.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16361..17302
                     /locus_tag="PWS53_000791"
     CDS             16361..17302
                     /locus_tag="PWS53_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871053.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17303..18346
                     /locus_tag="PWS53_000792"
     CDS             17303..18346
                     /locus_tag="PWS53_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871054.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18352..19422
                     /locus_tag="PWS53_000793"
     CDS             18352..19422
                     /locus_tag="PWS53_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871055.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19412..20365
                     /locus_tag="PWS53_000794"
     CDS             19412..20365
                     /locus_tag="PWS53_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871056.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20377..21387
                     /locus_tag="PWS53_000795"
     CDS             20377..21387
                     /locus_tag="PWS53_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0871057.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21672..22493)
                     /locus_tag="PWS53_000796"
     CDS             complement(21672..22493)
                     /locus_tag="PWS53_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0871058.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22590..22868)
                     /locus_tag="PWS53_000797"
     CDS             complement(22590..22868)
                     /locus_tag="PWS53_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871059.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23136..25145
                     /gene="metG"
                     /locus_tag="PWS53_000798"
     CDS             23136..25145
                     /gene="metG"
                     /locus_tag="PWS53_000798"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL0871060.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25624..26211)
                     /locus_tag="PWS53_000799"
     CDS             complement(25624..26211)
                     /locus_tag="PWS53_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871061.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26386..27708
                     /locus_tag="PWS53_000800"
     CDS             26386..27708
                     /locus_tag="PWS53_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0871062.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            27863..28639
                     /locus_tag="PWS53_000801"
     CDS             27863..28639
                     /locus_tag="PWS53_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0871063.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28632..29201
                     /gene="rnmV"
                     /locus_tag="PWS53_000802"
     CDS             28632..29201
                     /gene="rnmV"
                     /locus_tag="PWS53_000802"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL0871064.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29194..30078
                     /gene="rsmA"
                     /locus_tag="PWS53_000803"
     CDS             29194..30078
                     /gene="rsmA"
                     /locus_tag="PWS53_000803"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL0871065.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30529..31158
                     /locus_tag="PWS53_000804"
     CDS             30529..31158
                     /locus_tag="PWS53_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871066.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31282..32391)
                     /gene="ugpC"
                     /locus_tag="PWS53_000805"
     CDS             complement(31282..32391)
                     /gene="ugpC"
                     /locus_tag="PWS53_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL0871067.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32600..33022
                     /gene="mgsA"
                     /locus_tag="PWS53_000806"
     CDS             32600..33022
                     /gene="mgsA"
                     /locus_tag="PWS53_000806"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL0871068.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33345..33881
                     /locus_tag="PWS53_000807"
     CDS             33345..33881
                     /locus_tag="PWS53_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0871069.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(33921..35684)
                     /locus_tag="PWS53_000808"
     CDS             complement(33921..35684)
                     /locus_tag="PWS53_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871070.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35687..37402)
                     /locus_tag="PWS53_000809"
     CDS             complement(35687..37402)
                     /locus_tag="PWS53_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871071.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        12329 a         6624 c         7724 g        10761 t
ORIGIN      
        1 ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat
       61 tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc
      121 ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat
      181 tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca
      241 accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt
      301 ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa
      361 agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg
      421 ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc
      481 attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg
      541 ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca
      601 cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat
      661 cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat
      721 acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga
      781 tttccccagc gctacattta atttttcagt aactgataat tacattgaaa taactatacc
      841 aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc
      901 agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc
      961 tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa
     1021 cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat
     1081 cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag
     1141 tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga
     1201 ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa
     1261 tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa
     1321 caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag
     1381 accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga
     1441 tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca
     1501 gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa
     1561 tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa
     1621 taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg
     1681 caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa
     1741 agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga
     1801 cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat
     1861 tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg
     1921 tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa
     1981 tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag
     2041 caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct
     2101 tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac
     2161 tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt
     2221 ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc
     2281 atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc taactatgga
     2341 agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg attggccaaa
     2401 agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg ggaaataaga
     2461 aaactactct atcattacaa attttcataa actggcagat gttctaagta aacaagtatc
     2521 ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg atgaaagcct
     2581 acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt ctctttgtcg
     2641 tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc aggaagctgt
     2701 tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg ttttaaatcg
     2761 gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct tgatttcaat
     2821 aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac tgacgtaatc
     2881 gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc ataagcttct
     2941 tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt atcataaaaa
     3001 agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac aaatgacatc
     3061 gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga aagaggcagc
     3121 agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag gaaaatccgg
     3181 ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct tttccgtgag
     3241 gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg gatcaaagga
     3301 ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa ggaaaaaata
     3361 aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg atttttttat
     3421 tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat tattagtagg
     3481 agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac cattagggat
     3541 tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc ttcaaacata
     3601 tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt tttatgggtt
     3661 gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct ttatcttaaa
     3721 ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc ctcaaggtca
     3781 atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga aaatcgtcat
     3841 cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca acttggtcaa
     3901 agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg ggaaaatcgc
     3961 gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct atcttacatt
     4021 gactggtatt ctaacaatca taatgaaaca agttgaagga aaattaaatt attaccgcta
     4081 actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat ttggtgaaaa
     4141 aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag ctatcgtagg
     4201 tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag aaagttctga
     4261 cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta aaatgaaaga
     4321 acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga atatttttga
     4381 aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat acaccaaaaa
     4441 agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct atccttatca
     4501 gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta tgaatccacg
     4561 ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc agcaagtcga
     4621 gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca cccatgattt
     4681 agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc attaagaaag
     4741 gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg tcttcttctt
     4801 tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat caacgaagag
     4861 aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag agataaagac
     4921 gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca atatgggatt
     4981 acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa caatggaacg
     5041 atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa agtgcaattt
     5101 acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc aggaatcaca
     5161 aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc tggttatgat
     5221 gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga tgctgttttg
     5281 tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga tggtctgctg
     5341 atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga agacttcaac
     5401 ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa gtcagatagt
     5461 acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg tgaattccaa
     5521 aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca aataggggac
     5581 tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc ctgccaactc
     5641 cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg ttcaaaagcc
     5701 agtttttcgg tattaagcca aatttatgca aaaatgtaga aagccatttt cacaaaattt
     5761 ttcttactac tcgaaactaa gcgacttttt cacaacctcc tttacataat tttattttgt
     5821 tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata ggaaaggaag
     5881 agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg gggctctgct
     5941 tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggaga atcagtctgg
     6001 gatcgatttg tacggatacc tggaaagaca tttaaaggaa cgaacggtga tttggcagta
     6061 gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg tctgaaagct
     6121 taccgcttct ctgtagcttg gacacggatt tttcctaatg gacgaggaga agtcaatcaa
     6181 gctgggctcg ctttttatga gagattgatc gacgaattga ttgaaaacga tattgaacct
     6241 atgttgactt tatatcattg ggatcttcca caagcattgc aagatgaata taatggttgg
     6301 gaatcacgtc agatcattga agatttcact cattatgcag aaacattatt tgaggcattc
     6361 cgcgggaaag tccattactg gattagtttg aatgaacaaa atatcttcac ttcattaggt
     6421 tatttgttag cagcacatcc gccaggtgtg acagacccga aacgaatgta tgaagttaac
     6481 catattgcta atttagcaaa tgcatctgtc atcaataaat ttcacgagtt agaaatgcct
     6541 ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caattgacag taatcctgaa
     6601 aatgttctgg ctgctgaaaa tgcagaagac ctgatggcca attattggat ggatgtctat
     6661 atgtggggaa aatatccgat tgctgccatg aggttcttag aagaaaaagg atgggcacca
     6721 acgattgaag ctggcgatgc agaattgcta gaatctgcta aaccagattt tcttgggatc
     6781 aattactatc aaacggcgac gaatgcattc aatccattaa acggagttgg tgctggaaaa
     6841 atgaatacga caggaaaaaa aggttcatca gaagaaactg gagttcctgg aatgtataaa
     6901 aaagttgaga atccatttgt cgaacgaaca aattgggact gggagattga cccagaagga
     6961 ttaaggatcg gactgcgtcg tatcacaagc cgttaccgca tgccggtact gattacggaa
     7021 aatggcttag gagagtatga caagttgaca gaagataaac aaatccatga cgactatcga
     7081 atcaattact tacaaagcca cgtcaaagcc atcaaagaag caatttcgga tggagcagaa
     7141 gtcctaggct attgcacctg gtcttataca gatttgctta gctggctgaa cggttatcaa
     7201 aagcgatatg gatttgtata tgttgatcaa gacgaaacac aagaaggatc tttggaacga
     7261 tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg agagaacctt
     7321 tagttcacat acttaaaaca agaaggtacg gctggatatg gccgtgcctt ttcttgataa
     7381 aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga aagcgtcagt
     7441 tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact tgatgaaatg
     7501 agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag acgaaaaaaa
     7561 cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg tttgccttga
     7621 ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg attgcaaacg
     7681 gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta gccgtattag
     7741 gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga cgctggtttg
     7801 gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta tttggcaaac
     7861 tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc agtgatataa
     7921 aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc ctttatcatt
     7981 ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta ttactggcag
     8041 gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt atccaatcaa
     8101 ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct cgaaagcaag
     8161 cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg cgcttagctg
     8221 caaaagaagc tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg gtgagccagc
     8281 aaagggaaaa tactgctgat gaatggcagg aaccagaaaa tacagagccg gagcagctaa
     8341 gttttgttcc aatcgacagt tttcaagaaa atatccatcc tgcgaatcta gagaaaccag
     8401 taccagatac accaaaacag accaatacag cagaaggttt ttcagatgag ttgccagagg
     8461 atgacggtac ctcattagaa tttgagattg aagcagaaca ggaaaatcaa gactatgaat
     8521 taccgacagt tgatttgctt gattctatac caactgtgga tcaaagtgat gagtataaaa
     8581 aaattgaaaa aaatatcggc gtattagaac aaactttcca aagtttcggt gtcgatgcca
     8641 aggtcgtcaa agccagctta ggaccatccg tcacaaaatt cgaagtccag ccggctgtag
     8701 gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct ttagcggcga
     8761 aagatgtccg gatggaagca ccgattccag gtaaatcact gattggtatt gaagtaccaa
     8821 atggaaagat cagtatggtt tcctttagag aaatcatcga agcacaacca aaccacccag
     8881 ataaattact agaagtacct ttaggacgtg atgtttctgg acgggtacaa acggctgatc
     8941 tttcgaagat gcctcactta ctcgtggctg gttcgactgg tagtgggaag tcagtagcaa
     9001 ttaatgggat tatcacaagt atattaatgc gtgctaagcc ccatgaagta aaactgatga
     9061 tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg catacctcat ctgttgacgc
     9121 cggtcgtgac gaaccctaga aaagcagcgc aagccttaca aaaagtagta caagaaatgg
     9181 aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt tacaatgaat
     9241 ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca ttcatcgtag
     9301 tgatcgtaga tgaattagct gatttgatga tggtagccag caatgaagtt gaagatgcga
     9361 ttattcgttt agcgcaaatg gctcgggcag cggggattca tatgatccta gctactcaac
     9421 gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc agaatggcat
     9481 ttgcggtatc aagcggtaca gattctcgga cgatcattga ttctaatggg gcagaaaagc
     9541 tcttagggcg aggagatatg ctattcttgc caatgggtga gaataaaccg atccgtgtgc
     9601 aaggagcatt catttctgac cacgaagtag aacgggtcgt tcaatttgtt acagatcagc
     9661 aagaagctca ttatgaagag aaaatgatgc cgacagatga agttgaaacg acaggtgctc
     9721 cagaacagcc acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt gaaatgcaaa
     9781 cggcaagtat ttcccttctc caaagaagat ttcggatcgg ttacaaccga gcagcacgtt
     9841 tagtagatga actggaagct catggagttg ttggcccgtc agaaggaagc aagcctcgga
     9901 aagtattcat tgagcaggaa gaaacatcag aaacgttgcc ggacgataat gacatgtaaa
     9961 acttgtgtga tgaagggttg aaaaacgaat aaccaataaa aagacaaggc atgatgatac
    10021 caaaagaaaa aatggttttt ttccatcttt tctaatacgg tatgatgtct tgtcttttta
    10081 gtttttgtga gattaaagtt ctattcgatt tatttgtgaa gtattgcatt aaggaaatga
    10141 atcatgtata gtatgtagcg aaactttgag aaaaagaggg aaaatacttt ggaaaaacag
    10201 tccatttatg gcttgacgaa tgaagaatta atcaattggt ttatagaaaa cggcgagaaa
    10261 aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag taatttcaca
    10321 gaaatgacga atctatcgaa acaattgatc gaaaaattat cggagcattt tatcatcaat
    10381 cctctaaaac agatggtcgt ccaagaagct tcggatggta cagtaaaata tttgtttgaa
    10441 ctccctgata aaaacatgat tgagactgta ctgatgagac aagaatatgg cttgtcagtt
    10501 tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag cgggctgctc
    10561 aaaaagaacc gtgatctgac tgctggagaa attgttgcac agatcatgat ggtccaacat
    10621 tattttgatg agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat ggggatcggt
    10681 gagccatttg ataattacga caatgtaatg gactttttgc atatcatcaa tgatgccaaa
    10741 ggattggcaa ttggtgctcg gcacattaca gtttccacta gtggattagc acacaaaatc
    10801 aaggaattcg ctaataacgg cttgcaagtc aatctggcta tttccttgca cgcgccgaat
    10861 aatgaggtgc gaacatcgat catgcggatc aaccgaagtt tcccaattga gaaattgatg
    10921 gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa tcacgtttga atatatcatg
    10981 ttgaatcaag taaatgatag accagagcat gcgcaacaat tagcagatct attaaaagat
    11041 aagaaaaaat tagcatatgt caacttgatt ccatataatc cagtcagtga acatgatcaa
    11101 tattctcgca gtccaaaagc tgatgtattg agattttatg atgtattgaa gaaaaacggc
    11161 gtaaactgtg tgatacgtaa agaacatggt acagatattg atgcagcttg cggacaattg
    11221 cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt atcctactcg ttttttcaat
    11281 taccataaaa ggagcagttt tcttaattaa ggaactgttc aacttaaata agagaaaaga
    11341 ttctatagcg aaagctataa agcttttttt gacaagaaaa accctcatca agcaaagaaa
    11401 tgtcctcaat ttgatgacag cctgcttgat gggggtttta gcaattattt aactgatttt
    11461 cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa gaatggcctg
    11521 tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg ttgccatggc
    11581 tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg ttaacgatac
    11641 tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga actgctgatc
    11701 catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt atccctccat
    11761 tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat tatagttatt
    11821 gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga cttgttcaat
    11881 tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat aagtcaagag
    11941 acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc ctagatcatc
    12001 gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa tacctgccca
    12061 taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt gtacaggtgg
    12121 ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga cacataccgc
    12181 agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt tagctagttt
    12241 ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa acaggacgtc
    12301 tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag ttgaattacc
    12361 cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt ttattttgtt
    12421 ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc catattcttt
    12481 gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg gtaagaaaac
    12541 actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat tttaaagagt
    12601 gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg ataagtgatg
    12661 aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg aacaagtaca
    12721 aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta ccgattccct
    12781 ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg aaaacaggtt
    12841 ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata tcatgatgaa
    12901 caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt tgaataaagt
    12961 gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat agagatactg
    13021 attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga ctgttagaat
    13081 aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa taatccagta
    13141 ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata tggagacagc
    13201 tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc agcggtagac
    13261 caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac aggttgggaa
    13321 ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac tcgattagtc
    13381 aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt aggtacggat
    13441 gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac atttgataaa
    13501 gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa attaggttat
    13561 aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat ttggatggca
    13621 aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac agaagtaatc
    13681 agcttcaaga aataagaagg aatttacgaa aattacttct tgaatttttg tgaaattcca
    13741 acttatttcc gaagaagcta cttttggaac accgtgtacc agaggttgtg aaaaagtcgc
    13801 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt
    13861 tggcttaata ccgagaaact ggcttttgaa caccgtttat tcagataagg gactgtgaca
    13921 agacttttgt cacagtttct ttttgtttat tcttttttat ctttgtatgg caataataga
    13981 caatataacg aaaattattt aatggctttt ggaagaccat ggaaaacctt gtacaaagct
    14041 attttttctg aaaaattcct tgaaacctca cgaaaaaatc attattttat gaaaaagaca
    14101 aaaaaaagtg ttgatttttt aataattatt cattaaactc ttaaacaact tcagaaaata
    14161 ttaattattc tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg
    14221 gtttggttgc ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa
    14281 gcagtagttc agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc
    14341 ctcaagaaat gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat
    14401 tgaataacgt ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag
    14461 gagctagcga gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag
    14521 aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc
    14581 aacgaacagt agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa
    14641 atgcagaaga cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg
    14701 gtgactatga attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg
    14761 ctttcccatc atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg
    14821 catctgcaag tgataaagct gtttataatg gaccattcac attagaagat tttgatggac
    14881 caggaacaga tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag
    14941 taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat
    15001 tcaaagatgg ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa
    15061 acgatccagc ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac
    15121 gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg
    15181 atcgtgaagc tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga
    15241 tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa
    15301 aattagtttc ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat
    15361 tgggcgttga ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag
    15421 taatcgaata tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa
    15481 cacctgttcc attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac
    15541 taggcggatg ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag
    15601 gtaattcata caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag
    15661 cttcatcaac agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca
    15721 agatcatctc tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag
    15781 tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat
    15841 ggacatacgt tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc
    15901 aaatgtcgca acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata
    15961 gcagaaagag caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg
    16021 gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg
    16081 accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata
    16141 atcagaaacg gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa
    16201 tggttgatga gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg
    16261 atagattttt ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc
    16321 gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata
    16381 tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt
    16441 tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga
    16501 aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg
    16561 gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca
    16621 gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc
    16681 aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa
    16741 tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt
    16801 tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc
    16861 aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct
    16921 tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt
    16981 ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg
    17041 aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg
    17101 atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc
    17161 aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct
    17221 gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt
    17281 ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga
    17341 tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat
    17401 tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc
    17461 tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt
    17521 tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat
    17581 tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga
    17641 taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt
    17701 gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt
    17761 tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt
    17821 aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa
    17881 cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc
    17941 tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa
    18001 attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat
    18061 tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag
    18121 tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc
    18181 tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc
    18241 ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact
    18301 tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa
    18361 attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg
    18421 attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct
    18481 ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat
    18541 atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag
    18601 atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta
    18661 gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt
    18721 tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa
    18781 gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc
    18841 gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca
    18901 gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa
    18961 atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat
    19021 cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc
    19081 tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt
    19141 gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa
    19201 ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca
    19261 ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca
    19321 cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa
    19381 aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg
    19441 attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag
    19501 ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc
    19561 cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga
    19621 cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt
    19681 tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat
    19741 cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa
    19801 agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca
    19861 tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc
    19921 gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt
    19981 ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca
    20041 tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt
    20101 attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag
    20161 tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa
    20221 atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca
    20281 ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa
    20341 ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt
    20401 atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt
    20461 acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat
    20521 ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt
    20581 cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga
    20641 cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac
    20701 aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa
    20761 ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct
    20821 gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa
    20881 gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct
    20941 cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca
    21001 cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag
    21061 gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac
    21121 cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata tgactactta
    21181 tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat
    21241 aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc
    21301 aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg
    21361 ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt
    21421 agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa
    21481 agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt
    21541 ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa
    21601 aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt
    21661 tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg
    21721 attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg
    21781 gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc
    21841 gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg
    21901 actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg
    21961 ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg
    22021 atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg
    22081 attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga
    22141 tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca
    22201 ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca
    22261 aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa
    22321 actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag
    22381 ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt
    22441 gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt catctgacca
    22501 cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa
    22561 ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga
    22621 ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca
    22681 aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc
    22741 ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag
    22801 ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc
    22861 ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga
    22921 ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat
    22981 tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc
    23041 tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac
    23101 gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata
    23161 tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga
    23221 ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca
    23281 ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac
    23341 cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata
    23401 tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa
    23461 tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact
    23521 ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg
    23581 aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag
    23641 aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac
    23701 atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac
    23761 caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga
    23821 gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac
    23881 taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga
    23941 tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc
    24001 ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa
    24061 aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt
    24121 tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta
    24181 cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat
    24241 tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct
    24301 cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt
    24361 accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga
    24421 tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag
    24481 aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag
    24541 cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat
    24601 tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa
    24661 aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt
    24721 ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc
    24781 cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa
    24841 aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt
    24901 tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg
    24961 ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc
    25021 tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac
    25081 cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg
    25141 catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg
    25201 agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca
    25261 gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat
    25321 ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg
    25381 gcagatttta attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg
    25441 aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta
    25501 taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata
    25561 gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc
    25621 ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc
    25681 agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata
    25741 atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc
    25801 agctataaac caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg
    25861 atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat
    25921 attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa
    25981 aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc
    26041 gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa
    26101 taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac
    26161 ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc
    26221 ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt
    26281 aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt
    26341 tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta
    26401 aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca
    26461 ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg
    26521 ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta
    26581 gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa
    26641 ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct
    26701 gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat
    26761 cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca
    26821 aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg
    26881 attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa
    26941 gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt
    27001 gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa
    27061 gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc
    27121 aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct
    27181 tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc
    27241 ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat
    27301 gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag
    27361 tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc
    27421 cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta
    27481 ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat
    27541 aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta
    27601 ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata
    27661 ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca
    27721 acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa
    27781 aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta
    27841 agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca
    27901 gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat
    27961 ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta
    28021 tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga
    28081 aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat
    28141 tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag
    28201 acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact
    28261 tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca
    28321 tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc
    28381 atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt
    28441 accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag
    28501 aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg
    28561 gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg
    28621 tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg
    28681 atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa
    28741 tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt
    28801 ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc
    28861 aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg
    28921 gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag
    28981 acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa
    29041 tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt
    29101 acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac
    29161 tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca
    29221 ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga
    29281 caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat
    29341 gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc
    29401 aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct
    29461 gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat
    29521 ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat
    29581 ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg
    29641 gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga
    29701 acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca
    29761 ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa
    29821 ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg
    29881 acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat
    29941 ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat
    30001 ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg
    30061 tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca
    30121 tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt
    30181 gttaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt
    30241 aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg
    30301 tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa
    30361 attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac
    30421 actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt
    30481 atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat
    30541 tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg
    30601 gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta
    30661 tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt
    30721 ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt
    30781 gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag
    30841 tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct
    30901 ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat
    30961 tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac
    31021 agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat
    31081 atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg
    31141 aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata
    31201 ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc
    31261 acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa
    31321 gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc
    31381 tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc
    31441 agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc
    31501 aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc
    31561 ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc
    31621 atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga
    31681 tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc
    31741 gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc
    31801 tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat
    31861 ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct catccattaa
    31921 gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc
    31981 agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt
    32041 gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat
    32101 attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc
    32161 acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga
    32221 gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc
    32281 aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt
    32341 ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc
    32401 cacactttct tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact
    32461 tcaatgtcat gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt
    32521 taataaatat ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat
    32581 atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat
    32641 gatggtgaaa ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac
    32701 cgggacgact gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc
    32761 cggaccgtta ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt
    32821 ggtgattttt cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact
    32881 tatccgttta tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt
    32941 gctactaaga ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga
    33001 caagcatcca ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc
    33061 tatatgtagc tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg
    33121 ataatataaa caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag
    33181 tttctgtggg aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa
    33241 gaggatggga tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt
    33301 cgctgcatga tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt
    33361 tagacgcacg tatgattgct attatgggct tattgattgc attgatggtc actttgtccc
    33421 gactggtagc aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga
    33481 tcatgggcat tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag
    33541 taggaatggc cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta
    33601 ttgaaggagc aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca
    33661 ttctggctac gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg
    33721 cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa
    33781 cagtaatatg gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt
    33841 acaaaaaaat tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca
    33901 tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa
    33961 taaccatctt tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc
    34021 atcactaata tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg
    34081 gtttttcctt ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg
    34141 actgagctgg tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca
    34201 atcgtcaaca gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta
    34261 tctaattttt ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca
    34321 ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg
    34381 tcaaatagcc aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg
    34441 atagagcgga tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc
    34501 atcagcaaat taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt
    34561 tgacctggat aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa
    34621 aaagaaacat gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca
    34681 tctggcaaat caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca
    34741 gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac
    34801 tgacttgagt agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc
    34861 aatgccaata ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga
    34921 gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca
    34981 gcatttagca gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt
    35041 ttctggttac cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt
    35101 ctatcgaaat ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt
    35161 gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa
    35221 agaatgatga ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa
    35281 atattgtcca tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa
    35341 taacgaagtg gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat
    35401 aagtaagcta accgattgct taacacttgg atgagccatt gactgactac gttgattaaa
    35461 acgataccac cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct
    35521 ccaatcattt gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca
    35581 ttgatgattc ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga
    35641 atgaatcggc ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct
    35701 gtgatgcgac gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct
    35761 gagcagcaat cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga
    35821 cacgttggga gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat
    35881 ccaagtctgt ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct
    35941 tggcaatcaa agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc
    36001 cattttccat gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct
    36061 gagcgatttt cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa
    36121 ggttttcacg aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct
    36181 gacggagatg atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg
    36241 aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg
    36301 gaccaatgat tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa
    36361 gaccagaatc aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag
    36421 aaaaatcggg agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct
    36481 ctttcacccg atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa
    36541 cgatcagtgc caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt
    36601 gcaagctacc aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat
    36661 tcatgatcaa ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat
    36721 tgttcgctag atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa
    36781 atgcgcggat gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta
    36841 gtttttcttg cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga
    36901 ttgagaaaat aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag
    36961 ccatgaccac tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg
    37021 ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc
    37081 caaaatgatt caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta
    37141 actctgtacc aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa
    37201 tcagaccaat cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt
    37261 cattcatttt tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga
    37321 agacagcttc aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg
    37381 cataacgtaa taactgaatc atatgattcc tcctattatt tatttgtcca caattgaa
//
LOCUS       DANJJH010000017        37214 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000017 DANJJH010000000
VERSION     DANJJH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            59..808
                     /locus_tag="PWS53_000810"
     CDS             59..808
                     /locus_tag="PWS53_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0871072.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="PWS53_000811"
     CDS             complement(1232..1402)
                     /locus_tag="PWS53_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0871073.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="PWS53_000812"
     CDS             complement(1596..1775)
                     /locus_tag="PWS53_000812"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871074.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1785..2009)
                     /locus_tag="PWS53_000813"
     CDS             complement(1785..2009)
                     /locus_tag="PWS53_000813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871075.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            2162..3274
                     /locus_tag="PWS53_000814"
     CDS             2162..3274
                     /locus_tag="PWS53_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0871076.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="PWS53_000815"
     CDS             complement(3352..3645)
                     /locus_tag="PWS53_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL0871077.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="PWS53_000816"
     CDS             3813..4127
                     /locus_tag="PWS53_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL0871078.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="PWS53_000817"
     CDS             4148..6127
                     /locus_tag="PWS53_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0871079.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="PWS53_000818"
     CDS             6140..6466
                     /locus_tag="PWS53_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL0871080.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="PWS53_000819"
     CDS             6482..7000
                     /locus_tag="PWS53_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871081.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="PWS53_000820"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="PWS53_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0871082.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="PWS53_000821"
     CDS             complement(8436..9941)
                     /locus_tag="PWS53_000821"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL0871083.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="PWS53_000822"
     CDS             complement(10033..10623)
                     /locus_tag="PWS53_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0871084.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="PWS53_000823"
     CDS             10802..12448
                     /locus_tag="PWS53_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0871085.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="PWS53_000824"
     CDS             12497..13216
                     /locus_tag="PWS53_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0871086.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="PWS53_000825"
     CDS             13695..14642
                     /locus_tag="PWS53_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0871087.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="PWS53_000826"
     CDS             14654..15574
                     /locus_tag="PWS53_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871088.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="PWS53_000827"
     CDS             15615..17072
                     /locus_tag="PWS53_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0871089.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="PWS53_000828"
     CDS             17417..18031
                     /locus_tag="PWS53_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0871090.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="PWS53_000829"
     CDS             18024..19748
                     /locus_tag="PWS53_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0871091.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="PWS53_000830"
     CDS             19745..21196
                     /locus_tag="PWS53_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871092.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="PWS53_000831"
     CDS             complement(21669..23822)
                     /locus_tag="PWS53_000831"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL0871093.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="PWS53_000832"
     CDS             complement(23824..24870)
                     /locus_tag="PWS53_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871094.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="PWS53_000833"
     CDS             25020..26309
                     /locus_tag="PWS53_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL0871095.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="PWS53_000834"
     CDS             26322..29024
                     /locus_tag="PWS53_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL0871096.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="PWS53_000835"
     CDS             29009..29866
                     /locus_tag="PWS53_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0871097.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30836)
                     /locus_tag="PWS53_000836"
     CDS             complement(29937..30836)
                     /locus_tag="PWS53_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0871098.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31005..33176
                     /locus_tag="PWS53_000837"
     CDS             31005..33176
                     /locus_tag="PWS53_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0871099.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="PWS53_000838"
     CDS             33244..33585
                     /locus_tag="PWS53_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0871100.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="PWS53_000839"
     CDS             complement(33861..35615)
                     /locus_tag="PWS53_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871101.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="PWS53_000840"
     CDS             complement(35626..36462)
                     /locus_tag="PWS53_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0871102.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="PWS53_000841"
     CDS             complement(36464..37147)
                     /locus_tag="PWS53_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL0871103.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12005 a         6542 c         7633 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DANJJH010000018        37171 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000018 DANJJH010000000
VERSION     DANJJH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            67..435
                     /locus_tag="PWS53_000842"
     CDS             67..435
                     /locus_tag="PWS53_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL0871104.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            605..1036
                     /gene="mraZ"
                     /locus_tag="PWS53_000843"
     CDS             605..1036
                     /gene="mraZ"
                     /locus_tag="PWS53_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL0871105.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1057..2016
                     /gene="rsmH"
                     /locus_tag="PWS53_000844"
     CDS             1057..2016
                     /gene="rsmH"
                     /locus_tag="PWS53_000844"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL0871106.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2021..2422
                     /gene="ftsL"
                     /locus_tag="PWS53_000845"
     CDS             2021..2422
                     /gene="ftsL"
                     /locus_tag="PWS53_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL0871107.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2423..4615
                     /locus_tag="PWS53_000846"
     CDS             2423..4615
                     /locus_tag="PWS53_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0871108.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4641..5603
                     /locus_tag="PWS53_000847"
     CDS             4641..5603
                     /locus_tag="PWS53_000847"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL0871109.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5617..6978
                     /locus_tag="PWS53_000848"
     CDS             5617..6978
                     /locus_tag="PWS53_000848"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL0871110.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7004..8092
                     /gene="murG"
                     /locus_tag="PWS53_000849"
     CDS             7004..8092
                     /gene="murG"
                     /locus_tag="PWS53_000849"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL0871111.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8107..9327
                     /locus_tag="PWS53_000850"
     CDS             8107..9327
                     /locus_tag="PWS53_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL0871112.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9483..10808
                     /gene="ftsA"
                     /locus_tag="PWS53_000851"
     CDS             9483..10808
                     /gene="ftsA"
                     /locus_tag="PWS53_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL0871113.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10831..12072
                     /gene="ftsZ"
                     /locus_tag="PWS53_000852"
     CDS             10831..12072
                     /gene="ftsZ"
                     /locus_tag="PWS53_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL0871114.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12089..12766
                     /locus_tag="PWS53_000853"
     CDS             12089..12766
                     /locus_tag="PWS53_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL0871115.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12780..13379
                     /locus_tag="PWS53_000854"
     CDS             12780..13379
                     /locus_tag="PWS53_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL0871116.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13394..13681
                     /locus_tag="PWS53_000855"
     CDS             13394..13681
                     /locus_tag="PWS53_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL0871117.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13730..14512
                     /locus_tag="PWS53_000856"
     CDS             13730..14512
                     /locus_tag="PWS53_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL0871118.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14533..15240
                     /locus_tag="PWS53_000857"
     CDS             14533..15240
                     /locus_tag="PWS53_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL0871119.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15502..18300
                     /gene="ileS"
                     /locus_tag="PWS53_000858"
     CDS             15502..18300
                     /gene="ileS"
                     /locus_tag="PWS53_000858"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL0871120.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18349..19875)
                     /locus_tag="PWS53_000859"
     CDS             complement(18349..19875)
                     /locus_tag="PWS53_000859"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL0871121.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19890..20537)
                     /locus_tag="PWS53_000860"
     CDS             complement(19890..20537)
                     /locus_tag="PWS53_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL0871122.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20721..21050)
                     /locus_tag="PWS53_000861"
     CDS             complement(20721..21050)
                     /locus_tag="PWS53_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL0871123.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21227..21955
                     /gene="sfsA"
                     /locus_tag="PWS53_000862"
     CDS             21227..21955
                     /gene="sfsA"
                     /locus_tag="PWS53_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL0871124.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21971..22984
                     /locus_tag="PWS53_000863"
     CDS             21971..22984
                     /locus_tag="PWS53_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0871125.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22984..24261
                     /locus_tag="PWS53_000864"
     CDS             22984..24261
                     /locus_tag="PWS53_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0871126.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24324..27026
                     /locus_tag="PWS53_000865"
     CDS             24324..27026
                     /locus_tag="PWS53_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0871127.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27178..27495
                     /locus_tag="PWS53_000866"
     CDS             27178..27495
                     /locus_tag="PWS53_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0871128.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27525..27845
                     /locus_tag="PWS53_000867"
     CDS             27525..27845
                     /locus_tag="PWS53_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0871129.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27953..29413
                     /locus_tag="PWS53_000868"
     CDS             27953..29413
                     /locus_tag="PWS53_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0871130.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29481..29702
                     /locus_tag="PWS53_000869"
     CDS             29481..29702
                     /locus_tag="PWS53_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871131.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29733..29915
                     /locus_tag="PWS53_000870"
     CDS             29733..29915
                     /locus_tag="PWS53_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL0871132.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29915..30328
                     /locus_tag="PWS53_000871"
     CDS             29915..30328
                     /locus_tag="PWS53_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL0871133.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30451..31632
                     /locus_tag="PWS53_000872"
     CDS             30451..31632
                     /locus_tag="PWS53_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871134.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31703..31867)
                     /locus_tag="PWS53_000873"
     CDS             complement(31703..31867)
                     /locus_tag="PWS53_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871135.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            32021..32094
                     /locus_tag="PWS53_000874"
     tRNA            32021..32094
                     /locus_tag="PWS53_000874"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32055..32057,aa:Arg,seq:cct)
     gene            complement(32131..32331)
                     /locus_tag="PWS53_000875"
     CDS             complement(32131..32331)
                     /locus_tag="PWS53_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0871136.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(32636..33868)
                     /locus_tag="PWS53_000876"
     CDS             complement(32636..33868)
                     /locus_tag="PWS53_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL0871137.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            34122..34691
                     /locus_tag="PWS53_000877"
     CDS             34122..34691
                     /locus_tag="PWS53_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0871138.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(34869..35309)
                     /locus_tag="PWS53_000878"
     CDS             complement(34869..35309)
                     /locus_tag="PWS53_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL0871139.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            35467..36231
                     /gene="map"
                     /locus_tag="PWS53_000879"
     CDS             35467..36231
                     /gene="map"
                     /locus_tag="PWS53_000879"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL0871140.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            36263..>37171
                     /locus_tag="PWS53_000880"
     CDS             36263..>37171
                     /locus_tag="PWS53_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL0871141.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFS"
BASE COUNT        12291 a         6435 c         7837 g        10608 t
ORIGIN      
        1 aatgaaacag ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt
       61 acgttcatgg gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt
      121 ttgtttggtt gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa
      181 gcccctgatt tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca
      241 gggacaattt ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt
      301 gctgttttcc aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact
      361 tggcgtttgg tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata
      421 ctttcttacc tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca
      481 gcttgatttt taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa
      541 ggtggtagaa agtgggggat tgtggtagac taattttagt tagtggagga atggggggct
      601 ttcaatgttg atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc
      661 ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg
      721 tcttttcggc tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc
      781 gttggctaaa aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg
      841 cgaacttgat aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt
      901 gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg
      961 atggcaagat ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat
     1021 tgatttcgga ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat
     1081 acagtcatgt tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta
     1141 gactgtacac tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa
     1201 gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta
     1261 gcttcttatg tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa
     1321 gaacgcttga gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc
     1381 tctcctcagc tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat
     1441 atgcgcatgg atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac
     1501 catgaattag taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct
     1561 cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa
     1621 atcattaaac aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga
     1681 atttttcaag ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta
     1741 gaacaggcaa tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca
     1801 ttggaagaca gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca
     1861 ccgggacttc ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa
     1921 ccaatccttc cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta
     1981 cgtattgcag aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat
     2041 gcaagattac gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga
     2101 ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat
     2161 ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt
     2221 tttaacagtt atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca
     2281 aacagaaatc aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact
     2341 ttctcgtact gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga
     2401 caatttaagg aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa
     2461 gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag
     2521 tattggattg ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt
     2581 tgctggtacc tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa
     2641 agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc
     2701 ttattcaatt aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt
     2761 tgaagaaaag aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa
     2821 aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt
     2881 tggtagctat gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa
     2941 aaaagaaggt gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg
     3001 cgtcttttct tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt
     3061 tggtaaacta ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat
     3121 tatttatcaa aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa
     3181 agctgtcgac ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga
     3241 aacactgatg gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat
     3301 gaaagcaaaa accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac
     3361 aatggaagga ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta
     3421 tgaaccagga tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt
     3481 caacgagaat gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga
     3541 ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa
     3601 cgtcgggatg gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa
     3661 actgggattt ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc
     3721 tacattaaat attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa
     3781 tttccaaatg atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg
     3841 atatatcagc aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt
     3901 tctagggcaa cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt
     3961 tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt
     4021 atcggcaaaa aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga
     4081 tacagcctac ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt
     4141 gtatctaaca atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa
     4201 tccattgatg aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac
     4261 aaaaacagaa aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc
     4321 tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa
     4381 acaatctact gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg
     4441 tggtgataag ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg
     4501 gaaaatcctc ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact
     4561 tgctccatat gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga
     4621 gaaataggag agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt
     4681 gtgcaatgac aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag
     4741 gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa
     4801 tgggcggctt agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc
     4861 aaaaacagct aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa
     4921 tcggctttct tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga
     4981 agcagaaatt gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg
     5041 aaggttatcc aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg
     5101 gtattttcgc tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca
     5161 tcgatggatt agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt
     5221 ggcatcagca tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg
     5281 gtttcttcgt ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag
     5341 cgttgggcgg acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt
     5401 tgattggttt gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta
     5461 agcttacagg aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg
     5521 gatggtcgga gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga
     5581 tcacattatg gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa
     5641 aataagaaag tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta
     5701 catgaattgg gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa
     5761 gcacaagagc tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg
     5821 ttggatgaag aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta
     5881 gtagcaaaag cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa
     5941 gtagcggaat gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg
     6001 atgacaggac tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat
     6061 attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg
     6121 gaattgtcta gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt
     6181 accaatatct atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct
     6241 aaatggaacc ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag
     6301 tcagaactac aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa
     6361 gaagtgttgg aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa
     6421 ataatggaaa tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct
     6481 gctatttctg tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat
     6541 tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt
     6601 tataatgatt caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa
     6661 acgtctaagg tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta
     6721 attccttcat taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg
     6781 atggatgctg gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa
     6841 gcagttcctt tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg
     6901 aatgccagct gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct
     6961 gtgaaccgat taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac
     7021 aggcggagga acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac
     7081 tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat
     7141 cgtccctgat acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat
     7201 ttccatgcat aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa
     7261 aatcctcagt gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc
     7321 ggtagtgtac gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt
     7381 accaggtgtt accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa
     7441 agatgcggct tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca
     7501 agaagtagca gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa
     7561 gaaaacagtc ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc
     7621 agttttttta agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag
     7681 atattatgaa gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc
     7741 ttatatcaaa aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg
     7801 tgcaacttcg atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc
     7861 atatgttacg aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc
     7921 aaaaatgatt gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat
     7981 catgggtgat gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc
     8041 tgatgcttca aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg
     8101 gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac
     8161 aatttgactc cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac
     8221 caagagccaa aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa
     8281 aaaacggttc aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt
     8341 gaagagatca agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg
     8401 cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact
     8461 gtattgggta taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa
     8521 gcagttgcag tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa
     8581 ttgtcattag gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc
     8641 cttaaaaaag cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa
     8701 ttcgatttgg atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac
     8761 tacccagtga ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat
     8821 ctccctatct tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac
     8881 aatcagttat catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca
     8941 agcaataaaa atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc
     9001 aataatcttg cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa
     9061 ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg
     9121 gaagaatcgg gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac
     9181 gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac
     9241 ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca
     9301 actccgtcaa gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc
     9361 taaaaaccag taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat
     9421 tttaaaacgg acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat
     9481 tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg
     9541 tcgtggctga atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag
     9601 aagggattaa ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag
     9661 cagtacgaca agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc
     9721 cagcaaacat gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa
     9781 aggaaatcac cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac
     9841 ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg
     9901 gaatcaaaga tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta
     9961 caggacctaa aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg
    10021 tcaacgaaat ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa
    10081 aagattttgg tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc
    10141 atgataagca attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag
    10201 atatctctat tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg
    10261 gggatgcata cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc
    10321 agtcagaacc agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg
    10381 aacagatttt caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag
    10441 gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag
    10501 aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg
    10561 tatttacaaa tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat
    10621 tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag
    10681 aaacaacaaa cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg
    10741 aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt
    10801 ttgattaatt catcctggga ggaataagat atggaattct caattgataa taatatcaat
    10861 gacggcgcag taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga
    10921 atgattgaag aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca
    10981 ttaaaaaatt caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta
    11041 ggtgctggtt ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta
    11101 cgcgaagctt tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc
    11161 ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt
    11221 ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa
    11281 ggtatcgctc gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta
    11341 ttagaagttg tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt
    11401 ttacgacaag gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt
    11461 gactttgctg acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt
    11521 gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg
    11581 cttgaaacat ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat
    11641 atgacattgt tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat
    11701 gtaaatatca ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg
    11761 gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga
    11821 caagcacaaa tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca
    11881 acttcttcag aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt
    11941 cgtcctagag ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc
    12001 aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt
    12061 cgtaaaaaat aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca
    12121 agaaattcag gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc
    12181 tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa
    12241 ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc
    12301 tgaaataaag tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa
    12361 tgaaattgat tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg
    12421 gttagagaaa gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca
    12481 tggaatttca ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat
    12541 caaggttgtt gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa
    12601 agtgtttggt acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc
    12661 accatgtagt gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg
    12721 tgcaacattt atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa
    12781 tgccgctttt aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt
    12841 acgaatacca agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata
    12901 caacacagaa tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa
    12961 cggcgacttc acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa
    13021 ttgaactaca tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct
    13081 ctacacaacc taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt
    13141 ctgaagctat ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc
    13201 atttagtaga agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg
    13261 cactagatgg agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga
    13321 tggagattga cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag
    13381 aggaggaact acgatttata ctattgcact attacttcat ttacttcaac gggcagccta
    13441 tttctatagc attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca
    13501 atcagggcta ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg
    13561 tctgcctttg aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa
    13621 cttagccgta caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg
    13681 attgaagcaa caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa
    13741 tgtataccag cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat
    13801 tgaacaggtt caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc
    13861 ctatatttta gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg
    13921 atatgaacag gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga
    13981 ggaagatttt gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag
    14041 tcatgggaaa atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga
    14101 tatcatctcg gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt
    14161 tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac
    14221 agaaatgatt atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg
    14281 actagacagt gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat
    14341 cgaagctggt aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga
    14401 actattagat attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg
    14461 gaagacgaaa aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa
    14521 ggtggtttac tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa
    14581 atgcggggat ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat
    14641 gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa
    14701 cttgagtact tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat
    14761 acggctgata aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca
    14821 caaaacaaag cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact
    14881 gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt
    14941 gcgacagaaa ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg
    15001 atgcttgagt ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca
    15061 ttctccagct acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt
    15121 gacaatgaaa atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg
    15181 gcatttgata cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag
    15241 aacagaaaag cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac
    15301 actgcatggt gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca
    15361 gcggctgacc tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg
    15421 gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca
    15481 aataaaggag tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa
    15541 ttccctatgc gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa
    15601 aatcgaatct accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat
    15661 gatggacctc catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt
    15721 aaagacatta tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga
    15781 tgggatacac atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa
    15841 gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat
    15901 aagcaacgag cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt
    15961 acattagatc cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa
    16021 ggctatattt acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta
    16081 gcggaagctg aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat
    16141 gtcaaagacg gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca
    16201 ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg
    16261 gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca
    16321 gaaatcggtt gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat
    16381 atgactgcaa gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt
    16441 actttagacg ctggtactgg attagtccat actgctccag gacatgggga agatgactat
    16501 atcgctggta gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt
    16561 acagaagaag cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact
    16621 gatctattaa aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct
    16681 cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct
    16741 atcgataaat tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca
    16801 tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt
    16861 cagcgtgcat ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc
    16921 acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg
    16981 tttgaaaaag aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat
    17041 ggcgaattta caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat
    17101 gaagctgttt tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga
    17161 tcagaccaat atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt
    17221 gtagcaccat acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag
    17281 atgagtaaat ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca
    17341 gatatccttc gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg
    17401 gatatcttaa gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta
    17461 ttagcgaata cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt
    17521 tctgtagaca aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa
    17581 gggtacgaca aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt
    17641 gatctatcgt cattctacct ggattttgct aaagatgtag tttatattga agcagaagat
    17701 gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa
    17761 ttattgactc ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa
    17821 gaggagtacg ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa
    17881 ttgatggata cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa
    17941 gaagcaagaa acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca
    18001 aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc
    18061 gtttcaccag actactttga agttatggca gcagatgaac aagtgccaga taacgctatg
    18121 gtatttgatg atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt
    18181 cgccaagttc gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt
    18241 gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa
    18301 aagggaaaag ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa
    18361 ttttccacgt tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc
    18421 aagtagttcg aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg
    18481 ttgtgtttgg tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc
    18541 ttcccaatga gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta
    18601 agcttctggg ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc
    18661 cgtttcaaat ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg
    18721 ctgaatatag atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac
    18781 agatgttttg aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt
    18841 ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc
    18901 gatagtgaat ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc
    18961 ttcgcgatag cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac
    19021 gaagttttct aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt
    19081 ttctgtacgg atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac
    19141 aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc
    19201 cccgcgatct tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg
    19261 attgttccat tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat
    19321 catttctttt cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct
    19381 gatttgctgg ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat
    19441 caaacgattg aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt
    19501 tccaaaaaat tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg
    19561 tccatctaat cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc
    19621 atgggattga tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa
    19681 tgcacggatt gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc
    19741 ggttccgatt acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga
    19801 cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt
    19861 tttttcatta gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc
    19921 gccttaaagc tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa
    19981 gctgcgatgt caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa
    20041 tccaacaatt ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag
    20101 gactccaatg tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct
    20161 ccatgaggac aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc
    20221 gaagtcacat gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa
    20281 tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc
    20341 agtccgcttg cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc
    20401 acaagtttag aaatcatttc actgacagat gcagctgaga catctagact agatacgatt
    20461 tgtttattgt tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct
    20521 tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg
    20581 taacaaaaaa aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt
    20641 ttttacaaca aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat
    20701 atatatacat tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat
    20761 tcgtcataaa gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt
    20821 acgactaaac gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc
    20881 aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac
    20941 catgtacctt gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag
    21001 taaataaaac ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc
    21061 accaaatcac tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca
    21121 ttgtaccgaa taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt
    21181 ctacaaatta tgataggata catagtagga agaaaggtga cataatttga agtatcagca
    21241 agtggaaata gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa
    21301 agacggagaa ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat
    21361 accaggcgct gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga
    21421 tctgattgct gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg
    21481 gctggcaatt gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt
    21541 tacggtatac aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac
    21601 ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat
    21661 tggtgctttt cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta
    21721 tgccgcacaa gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca
    21781 tctggcaacg atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca
    21841 gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt
    21901 caagcaggcc attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga
    21961 aggtaaaata atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt
    22021 ccaagcagct ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga
    22081 aaaagcacag gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa
    22141 gacttttttc aatattactc atatggacac catttatatt gcttctccga atagtcttca
    22201 ctttagtcaa gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc
    22261 tttttcgacg ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta
    22321 tttttttgaa gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt
    22381 accattaaaa gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta
    22441 tgaccaagta ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc
    22501 attgatggat ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa
    22561 agaagtccat tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat
    22621 cttacgttat gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt
    22681 accgtcagag atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga
    22741 acaagcacaa tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc
    22801 agaaaatcca atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga
    22861 tccagcttgg ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt
    22921 gatttatgat ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt
    22981 ttaatgaacg aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa
    23041 gggtttatct caccttctct tattcaacga gcagtttttc agccactaag taataaacaa
    23101 agtatagtag cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta
    23161 ctactaaacg tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa
    23221 ctagctattc aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc
    23281 caatctttgg tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca
    23341 gaaatcttga ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa
    23401 gcacatcaag tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac
    23461 agccaaatca ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt
    23521 tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag
    23581 acaattgacg tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt
    23641 gtaccgatac gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat
    23701 ggtctgattt tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat
    23761 agaggtgtac cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg
    23821 ctagaacaat tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga
    23881 ggtttagata ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa
    23941 gcttatttgc atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca
    24001 ctcgtacaag ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca
    24061 cttgaagaga tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat
    24121 gcagctcctg cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca
    24181 gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat
    24241 aaaggagcaa ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa
    24301 ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga
    24361 cagaaaacca acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa
    24421 gcaatgcaag ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg
    24481 gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg
    24541 actcttctac gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca
    24601 gcatacagca ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt
    24661 ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg
    24721 attcagagat gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgctc
    24781 aaaatccgca actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag
    24841 cagctcaaga tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg
    24901 agatccaccg tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta
    24961 tttaccgtcc gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg
    25021 cgacgactga atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa
    25081 tcaccttgcc gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt
    25141 ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc
    25201 ttgccttcat gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac
    25261 tagtctgtgc aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc
    25321 gaaaagaaga actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg
    25381 aacgtctcga ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg
    25441 ctgatgttgt ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg
    25501 aaaaagtcac ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt
    25561 caaaagatgt ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc
    25621 ccaaagatct tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg
    25681 aaaaacggtt agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc
    25741 aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg
    25801 tacggaaaaa ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag
    25861 aagaagaaca agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta
    25921 tcaatctagg agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc
    25981 acggcagaca gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag
    26041 tagggaaagc ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt
    26101 tacaatatgt caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata
    26161 tgggatcatt aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag
    26221 ctatcacgat gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa
    26281 gaagcttaga agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc
    26341 agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct
    26401 ttactggtga aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa
    26461 ataaggtaga actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg
    26521 atgccttatt ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc
    26581 aaaatatccc atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa
    26641 agcgtatagc tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat
    26701 taagcgatgt cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga
    26761 tccagaccga tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt
    26821 tagccttcat gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt
    26881 ctattttctc aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac
    26941 tagaacgaaa atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt
    27001 ttcttgaaaa taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc
    27061 tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt
    27121 tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg
    27181 gcaaaaaaaa caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca
    27241 aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc
    27301 tcagatgcag ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa
    27361 gtacgtttca tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat
    27421 gtcatcaata tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa
    27481 aatctaatga aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa
    27541 atctagaagc catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg
    27601 aagcgattgc ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg
    27661 cagaagaatc acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac
    27721 aaggaaatca tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat
    27781 ctattgcgtt tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg
    27841 aataaataca acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt
    27901 tgttataagg agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg
    27961 actatcaagt tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa
    28021 acatttagtt gctttgcgtg atgcttttat cggcacactg cccgcaacaa tggcgggatc
    28081 agtcgctgta atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta
    28141 tgctgacagt ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat
    28201 caatggtttt gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg
    28261 ggggtataat cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt
    28321 agctaccttg attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa
    28381 tgttccttca aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga
    28441 tggcacactg tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc
    28501 ttattttaca gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct
    28561 tgccaacatt acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc
    28621 agccatttta ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg
    28681 tcgtgtagct gatgggcaac tgatcattga tttggtgcaa aaatatattg cagagccatt
    28741 cctaggatta tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt
    28801 ctggttcttt ggtattcatg gaccaaatgt attggcacca gtgctagagg gaatctgggg
    28861 acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac
    28921 accagccgtg ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt
    28981 cgatgctttt gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt
    29041 gattttctct aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat
    29101 cttcaatatc aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt
    29161 tatcccattc ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct
    29221 tggcctagtc gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc
    29281 attcttagca acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt
    29341 gactttcttg atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg
    29401 cgaacaacaa taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga
    29461 attttcgagg aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca
    29521 aagaattgtt agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag
    29581 gaatcaagct gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa
    29641 aggaactaat caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt
    29701 agaaaatggt ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta
    29761 gtattggttc tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt
    29821 atagtatggt cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga
    29881 aaaaaagaga agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat
    29941 tccagcatcg tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc
    30001 gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc
    30061 gcaaaataac cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt
    30121 tcgtacatcg acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga
    30181 ttcgacctgt gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt
    30241 gaatgatatg cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat
    30301 gctagatatg atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga
    30361 aaaatgaagt aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc
    30421 actagctttt ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta
    30481 tcttcgattg aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct
    30541 gaaaggcgag aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat
    30601 gaggaggatc ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga
    30661 caagagtacc taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa
    30721 ggcgggatca atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc
    30781 ttatcttatc tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca
    30841 tttgataaaa tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct
    30901 ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa
    30961 cagatgccgg aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa
    31021 ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg
    31081 ggatatatcg tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg
    31141 gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa
    31201 acatttgaat attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca
    31261 tttgcttctg taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt
    31321 ttggaaaatt atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat
    31381 cattatgcga aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat
    31441 ctctcaaaag tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg
    31501 cagctattat tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg
    31561 acaaaagaat atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat
    31621 gactattttt gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc
    31681 atttggcatt tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt
    31741 tgttttcttt tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct
    31801 tttcccgatt ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat
    31861 cattcattgg aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta
    31921 aggactagtg gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc
    31981 aagtaaaaaa caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga
    32041 tagagcaatc gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtacaaa
    32101 taacccctgt atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc
    32161 taaactgcta tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa
    32221 aatagatgca tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa
    32281 attgattact aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata
    32341 gattcgaatt aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg
    32401 ttcttaactc tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag
    32461 atgacttttt attattttta gtaatagctg atgggttaat acttacaact ttccttttca
    32521 aagtaccata taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc
    32581 ttagatcctt tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag
    32641 cccaatcgcc atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat
    32701 ccatcttgtc agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt
    32761 ctgctaattc ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac
    32821 ctagtgctaa gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag
    32881 attgagaaat aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt
    32941 catctgtatc aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa
    33001 cttttccatc tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg
    33061 gctttgtact tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt
    33121 ccgtcggcat atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc
    33181 aaagatggtt tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg
    33241 cagaaaattg ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct
    33301 tccatgctaa gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata
    33361 atgcctcaac ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac
    33421 cagccgcagc cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca
    33481 ttagtgcttt gccattcgct gattgataag acgctactcg atggctatct acaccagcaa
    33541 atgcatcggg atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat
    33601 ccgcttgatc gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa
    33661 gaaattctct ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt
    33721 tataagcttc ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa
    33781 caactgtgtg tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt
    33841 ttagaagttc attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt
    33901 aactattagc atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag
    33961 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
    34021 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaca tgttacaatg aaattaatca
    34081 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
    34141 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
    34201 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
    34261 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
    34321 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
    34381 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
    34441 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
    34501 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
    34561 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
    34621 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
    34681 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
    34741 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
    34801 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
    34861 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
    34921 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
    34981 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
    35041 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
    35101 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
    35161 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
    35221 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
    35281 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
    35341 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
    35401 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
    35461 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
    35521 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
    35581 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
    35641 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
    35701 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
    35761 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
    35821 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
    35881 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
    35941 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
    36001 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
    36061 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
    36121 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
    36181 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
    36241 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
    36301 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
    36361 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
    36421 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
    36481 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
    36541 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
    36601 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
    36661 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
    36721 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
    36781 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
    36841 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
    36901 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
    36961 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
    37021 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
    37081 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
    37141 atggttcaat aaattgaaaa ctaaattttc t
//
LOCUS       DANJJH010000019        35398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000019 DANJJH010000000
VERSION     DANJJH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            8..649
                     /locus_tag="PWS53_000881"
     CDS             8..649
                     /locus_tag="PWS53_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871142.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="PWS53_000882"
     CDS             708..1148
                     /locus_tag="PWS53_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871143.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="PWS53_000883"
     CDS             1160..1828
                     /locus_tag="PWS53_000883"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0871144.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="PWS53_000884"
     CDS             1892..2515
                     /locus_tag="PWS53_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL0871145.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="PWS53_000885"
     CDS             2571..2957
                     /locus_tag="PWS53_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL0871146.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="PWS53_000886"
     CDS             3170..4561
                     /locus_tag="PWS53_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL0871147.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="PWS53_000887"
     CDS             5172..5540
                     /locus_tag="PWS53_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL0871148.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="PWS53_000888"
     CDS             5720..6661
                     /locus_tag="PWS53_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL0871149.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="PWS53_000889"
     CDS             6730..7461
                     /locus_tag="PWS53_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL0871150.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="PWS53_000890"
     CDS             7605..9785
                     /locus_tag="PWS53_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL0871151.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="PWS53_000891"
     CDS             9805..10287
                     /locus_tag="PWS53_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL0871152.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="PWS53_000892"
     CDS             10303..11064
                     /locus_tag="PWS53_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0871153.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="PWS53_000893"
     CDS             11195..12529
                     /locus_tag="PWS53_000893"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL0871154.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="PWS53_000894"
     CDS             12836..13321
                     /locus_tag="PWS53_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL0871155.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="PWS53_000895"
     CDS             13314..13997
                     /locus_tag="PWS53_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL0871156.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="PWS53_000896"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="PWS53_000896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL0871157.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="PWS53_000897"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="PWS53_000897"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL0871158.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="PWS53_000898"
     CDS             17613..18242
                     /locus_tag="PWS53_000898"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL0871159.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="PWS53_000899"
     CDS             18294..18626
                     /locus_tag="PWS53_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0871160.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="PWS53_000900"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="PWS53_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL0871161.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="PWS53_000901"
     CDS             19452..20825
                     /locus_tag="PWS53_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0871162.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="PWS53_000902"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="PWS53_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL0871163.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="PWS53_000903"
     CDS             21880..22320
                     /locus_tag="PWS53_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0871164.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="PWS53_000904"
     CDS             22464..23165
                     /locus_tag="PWS53_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL0871165.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="PWS53_000905"
     CDS             23162..24283
                     /locus_tag="PWS53_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL0871166.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="PWS53_000906"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="PWS53_000906"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL0871167.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="PWS53_000907"
     CDS             25662..26570
                     /locus_tag="PWS53_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL0871168.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="PWS53_000908"
     CDS             26605..26877
                     /locus_tag="PWS53_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL0871169.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="PWS53_000909"
     CDS             26888..27934
                     /locus_tag="PWS53_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL0871170.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="PWS53_000910"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="PWS53_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL0871171.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="PWS53_000911"
     CDS             complement(30945..31631)
                     /locus_tag="PWS53_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871172.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="PWS53_000912"
     CDS             complement(31609..32103)
                     /locus_tag="PWS53_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0871173.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="PWS53_000913"
     CDS             complement(32123..33343)
                     /locus_tag="PWS53_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871174.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="PWS53_000914"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="PWS53_000914"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL0871175.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPESVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6249 c         7612 g         9690 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aatctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DANJJH010000020        33816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000020 DANJJH010000000
VERSION     DANJJH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..635)
                     /locus_tag="PWS53_000915"
     CDS             complement(<1..635)
                     /locus_tag="PWS53_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0871176.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTK"
     gene            complement(628..1293)
                     /gene="pgmB"
                     /locus_tag="PWS53_000916"
     CDS             complement(628..1293)
                     /gene="pgmB"
                     /locus_tag="PWS53_000916"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0871177.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            complement(1286..3580)
                     /locus_tag="PWS53_000917"
     CDS             complement(1286..3580)
                     /locus_tag="PWS53_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0871178.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            3840..6005
                     /locus_tag="PWS53_000918"
     CDS             3840..6005
                     /locus_tag="PWS53_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0871179.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            6309..7142
                     /locus_tag="PWS53_000919"
     CDS             6309..7142
                     /locus_tag="PWS53_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL0871180.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(7244..8944)
                     /locus_tag="PWS53_000920"
     CDS             complement(7244..8944)
                     /locus_tag="PWS53_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871181.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            9451..10611
                     /locus_tag="PWS53_000921"
     CDS             9451..10611
                     /locus_tag="PWS53_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0871182.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            10627..11070
                     /locus_tag="PWS53_000922"
     CDS             10627..11070
                     /locus_tag="PWS53_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871183.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11553..12083)
                     /locus_tag="PWS53_000923"
     CDS             complement(11553..12083)
                     /locus_tag="PWS53_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL0871184.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            12188..12739
                     /locus_tag="PWS53_000924"
     CDS             12188..12739
                     /locus_tag="PWS53_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0871185.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(12892..13365)
                     /locus_tag="PWS53_000925"
     CDS             complement(12892..13365)
                     /locus_tag="PWS53_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0871186.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            13503..14975
                     /locus_tag="PWS53_000926"
     CDS             13503..14975
                     /locus_tag="PWS53_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0871187.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            14972..15682
                     /locus_tag="PWS53_000927"
     CDS             14972..15682
                     /locus_tag="PWS53_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL0871188.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            16034..16318
                     /locus_tag="PWS53_000928"
     CDS             16034..16318
                     /locus_tag="PWS53_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL0871189.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(16599..17018)
                     /locus_tag="PWS53_000929"
     CDS             complement(16599..17018)
                     /locus_tag="PWS53_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL0871190.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(17020..17604)
                     /locus_tag="PWS53_000930"
     CDS             complement(17020..17604)
                     /locus_tag="PWS53_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL0871191.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(17651..19126)
                     /locus_tag="PWS53_000931"
     CDS             complement(17651..19126)
                     /locus_tag="PWS53_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL0871192.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            19307..19618
                     /locus_tag="PWS53_000932"
     CDS             19307..19618
                     /locus_tag="PWS53_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871193.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(19733..22069)
                     /locus_tag="PWS53_000933"
     CDS             complement(19733..22069)
                     /locus_tag="PWS53_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871194.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            22211..22879
                     /locus_tag="PWS53_000934"
     CDS             22211..22879
                     /locus_tag="PWS53_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871195.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            22882..23925
                     /locus_tag="PWS53_000935"
     CDS             22882..23925
                     /locus_tag="PWS53_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0871196.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(24162..25043)
                     /locus_tag="PWS53_000936"
     CDS             complement(24162..25043)
                     /locus_tag="PWS53_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0871197.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            25202..25930
                     /locus_tag="PWS53_000937"
     CDS             25202..25930
                     /locus_tag="PWS53_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL0871198.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            26086..26403
                     /locus_tag="PWS53_000938"
     CDS             26086..26403
                     /locus_tag="PWS53_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0871199.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            26396..27004
                     /locus_tag="PWS53_000939"
     CDS             26396..27004
                     /locus_tag="PWS53_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL0871200.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            27059..27850
                     /locus_tag="PWS53_000940"
     CDS             27059..27850
                     /locus_tag="PWS53_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL0871201.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            27988..28500
                     /locus_tag="PWS53_000941"
     CDS             27988..28500
                     /locus_tag="PWS53_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL0871202.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            28526..29398
                     /locus_tag="PWS53_000942"
     CDS             28526..29398
                     /locus_tag="PWS53_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0871203.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            29574..29936
                     /locus_tag="PWS53_000943"
     CDS             29574..29936
                     /locus_tag="PWS53_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0871204.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            29945..30697
                     /locus_tag="PWS53_000944"
     CDS             29945..30697
                     /locus_tag="PWS53_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871205.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            30711..32513
                     /locus_tag="PWS53_000945"
     CDS             30711..32513
                     /locus_tag="PWS53_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871206.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            32790..>33816
                     /locus_tag="PWS53_000946"
     CDS             32790..>33816
                     /locus_tag="PWS53_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0871207.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKI"
BASE COUNT        10603 a         5967 c         6956 g        10290 t
ORIGIN      
        1 ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac accatctaaa
       61 aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag gttgaagtag
      121 gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt tgtcatctct
      181 aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg ataggtagaa
      241 aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa aacttctact
      301 tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga tccgcagttt
      361 ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac aggtccgaca
      421 gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta agacaataaa
      481 acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg ggcaccgtaa
      541 ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat ttcagtctct
      601 ggaactggta cgacggagat ctctggatta tccatggtca tgacactttt ctttcaagta
      661 atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa ggtcttcagc
      721 acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac cggcttgcgc
      781 gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg caaggaggaa
      841 gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag catcaaagta
      901 aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag aagcaagagc
      961 aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg ggaagacatc
     1021 ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt tcgctaattc
     1081 tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa gcaaagagag
     1141 tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga tcgtaatgcc
     1201 gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat cggtaatgac
     1261 accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt agcgttattt
     1321 ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga ggaaaacctt
     1381 ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa cgtccgcgat
     1441 aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg aatctcaact
     1501 gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag ctaccagtca
     1561 tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa tccaagcgag
     1621 ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct gctaaaatcg
     1681 aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag aaatcaaagt
     1741 ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc tgtagtacat
     1801 ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc tgattcagag
     1861 gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag aatgtgtcat
     1921 gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct gcgatatctt
     1981 tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga taagttaaat
     2041 aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac caattgttat
     2101 tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat ttatttgctc
     2161 gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa acttccagtc
     2221 cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag attgcatagg
     2281 caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta tggcattcga
     2341 caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc tgagcgttgt
     2401 ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt acttcaggtt
     2461 tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa gtggctccgc
     2521 cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct tcgccgtagt
     2581 aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct tcgtctcctt
     2641 cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc tcttcttttg
     2701 cttggatata attgtttgtc tggttcgtta attctgagac agctttttct gcttgctgcg
     2761 cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac catgtctcac
     2821 cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg tgatagtcag
     2881 ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta aattgttcaa
     2941 tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct atagttccac
     3001 gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga acatttccat
     3061 ccaattttga tacgagtttg actgttccgc taccttcaag catttctata gttaaataga
     3121 tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg cgtactttat
     3181 tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata tctaattccc
     3241 agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg atcgaaatat
     3301 ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga taaccatttt
     3361 tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa gtaccttgat
     3421 ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa ccgtttccaa
     3481 tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc gtgctgattt
     3541 tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc ctaatgagct
     3601 gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa aagaaaatat
     3661 ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa gggaacacac
     3721 gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc atttacaaaa
     3781 aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa
     3841 tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta atggttgtgg
     3901 tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt cccttgatca
     3961 atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa
     4021 ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa
     4081 gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg atcactgggg
     4141 ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc cacactctgt
     4201 tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca ccagctttga
     4261 atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct tataataagt
     4321 actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag cgctttgttc
     4381 cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa
     4441 acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa gaaagcgcac
     4501 cgattctagc accgttttta tttggaacat tagaacgttt actattgcca tttggattgc
     4561 atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat gagattttat
     4621 caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg gcttgggcca
     4681 ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt gtgttgcata
     4741 actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt atcttgatgg
     4801 gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa
     4861 tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca ttggagttca
     4921 tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa ggggctgcat
     4981 ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc gaattgctga
     5041 ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt
     5101 gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc aaaaaattca
     5161 actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa accgttgtat
     5221 ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat ttgctgggag
     5281 gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg
     5341 atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg ggattgatcg
     5401 tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg aaatcagata
     5461 tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt tctacaggaa
     5521 atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt tccgcagctt
     5581 ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa aaaatgatgg
     5641 gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag
     5701 tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag
     5761 ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt gagattgctg
     5821 tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat cttgatgtga
     5881 tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat aaagtagaag
     5941 aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc tccattaaaa
     6001 tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa
     6061 atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca gtttgaatac
     6121 tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca
     6181 aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga acactatttg
     6241 ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg aagacaagga
     6301 gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca gaaccagaga
     6361 aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg attgctttac
     6421 aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta atgaagttct
     6481 gtcaggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt ttagttcagc
     6541 ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac atcggttatg
     6601 ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt caggtcctta
     6661 ccgtctccca atctcaaaag aaagaagatt atcagacacg caagatcttg attgggaaaa
     6721 caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg tggacagata
     6781 aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg gaaactcgcg
     6841 taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt tattatactg
     6901 taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag
     6961 cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag
     7021 attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt tttgatggga
     7081 tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat gtgaacgaat
     7141 aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca aacgattata
     7201 ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca cgtaaaagct
     7261 tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa gtaagagaaa
     7321 gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg attccttttt
     7381 tctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat gtttttccat
     7441 tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt ttgtccccag
     7501 tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt
     7561 tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc ttatagattg
     7621 gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata tctttgctga
     7681 agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga ctgacaaatg
     7741 tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt gctgaaaaaa
     7801 taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac tggttcagtg
     7861 aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga aatggaaact
     7921 ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga atcaagctag
     7981 tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat aaggtgttct
     8041 tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga aaggccttag
     8101 ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt tctctgttaa
     8161 caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc caaatatttg
     8221 ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct tttccttttt
     8281 cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct aacatgatcg
     8341 aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg ataacaggga
     8401 actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa aaacaagtcc
     8461 cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta ctcatgaata
     8521 aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt aaaatcagca
     8581 tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa tgaaaccagc
     8641 tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg taaacggcat
     8701 tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg aaatccccat
     8761 atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca tcagcaccca
     8821 aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg
     8881 ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta gataaattca
     8941 tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa tatttatgaa
     9001 ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat aagttttgtt
     9061 gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta gctttttcat
     9121 gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca ctagcctaga
     9181 aaatatgaga agctgttttc tgactagcgc tcttattact caaagcataa cgcagttttc
     9241 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaacccaa
     9301 caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca ggacagcttt
     9361 ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata ataaaagaaa
     9421 gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt
     9481 cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact gatcgcttta
     9541 tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat cgaacaagta
     9601 caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga gaaaaaacaa
     9661 cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa gaacagaaat
     9721 gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa tatacctaag
     9781 atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga aaatggagca
     9841 ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc agtgatctct
     9901 gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt agaaaacgga
     9961 gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa aaatatacaa
    10021 gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga cttagttacc
    10081 ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac aggacatcgg
    10141 attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg
    10201 cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt agtgtttatc
    10261 ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc atttattatt
    10321 agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca taagaaaaaa
    10381 acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg gaaagtcaag
    10441 ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc taaagttgct
    10501 gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga ggggcgaaaa
    10561 cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata agtaaggaga
    10621 gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc tgttctatcg
    10681 atttggctgc atgctaactt ggattctcat gaattcattc catcaactta ttgggagaat
    10741 caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc agaaatagat
    10801 caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa
    10861 agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa agaaaccaat
    10921 aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt ctatctttca
    10981 caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga ggaacttgtg
    11041 atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa tgctcccagc
    11101 ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact gtgtgaatat
    11161 gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt attagctcaa
    11221 aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc aggtcaaaac
    11281 gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg taataagccc
    11341 actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac tcaaagcata
    11401 acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc ggtattagct
    11461 caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata ctcaggtcag
    11521 aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata attttcatcg
    11581 aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc gctaataaca
    11641 tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc aggaacaagt
    11701 aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat atgttcgttt
    11761 tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa gatcattccg
    11821 tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt tggctctgtc
    11881 caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg gtcttttacc
    11941 caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc atttttggca
    12001 taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc aataaaatct
    12061 tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa catgtaataa
    12121 actacatgtt ttcgattact catttatgat agaatggatt taaagatttg tcaaggaggg
    12181 aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat taagtaagaa
    12241 gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag aattttggca
    12301 agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag gaatcattca
    12361 gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga
    12421 agggaaggaa caattggcag agtggattca tacaccaacc aatgaactac cggttaatcg
    12481 agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg cattagtaga
    12541 aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat tagagcgaaa
    12601 aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact acctgatttt
    12661 agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg tattagaaga
    12721 aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct ttctatatga
    12781 aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt ttcaagtatt
    12841 tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg tttagagttc
    12901 ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt aagttaattc
    12961 gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga agttaccgat
    13021 ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac
    13081 tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag cttttgacaa
    13141 agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg taggtaaacg
    13201 atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct tttgttccca
    13261 ataccttaat tgacgtggag acacacctac gatatcactt aattcactga tgccaacaac
    13321 caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg tcactccttt
    13381 tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta ggttgacaaa
    13441 tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa agaaggagaa
    13501 atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg acacttttag
    13561 ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta ttgacgacag
    13621 cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa tggttaacga
    13681 ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg atcaataaat
    13741 tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga acgattactt
    13801 gctttacagc acctaatttt agtacattac taatcggtcg cttgatccaa gcttgcggcg
    13861 tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa
    13921 gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct ttaggcccta
    13981 cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt atggtactgc
    14041 caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt attccattgt
    14101 caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc ggtagtttac
    14161 tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta attggtttct
    14221 tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat ttggaacatc
    14281 cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct gttttagctg
    14341 gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat attcaaaata
    14401 ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca ttgatcatgg
    14461 gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca
    14521 tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg acaaaaacga
    14581 ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc tccatggtca
    14641 tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc agtcatggga
    14701 cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct attttgatca
    14761 gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg gataatacgc
    14821 cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat gccgcgttta
    14881 tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg aagaaaaaag
    14941 aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga ttgtcagctt
    15001 gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt
    15061 gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt tgtccaatca
    15121 ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca gtgctgattc
    15181 ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa aagtgtatct
    15241 ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata acgaaacaaa
    15301 taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt attgggaata
    15361 caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg aatatgacag
    15421 ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg accaagcagc
    15481 caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag caaaagctta
    15541 tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca aagcagagca
    15601 agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg caaaattagt
    15661 gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg aaaaagctgc
    15721 tctgcatcaa gcagtaagaa caatcagata aaaatagcac tccatatttt tcatctgatt
    15781 gggcttagtg ccgtagatgc aagcttttga acaccgttta aggaagaggt tgtgagcctg
    15841 ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgact
    15901 attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcgaaa aaaggaactg
    15961 cgacaagact tttgtcacag ttccttcttt tttttgctat gctatactag atgtaaaaaa
    16021 taggagaaat aaaatgaggg aaatttattc ttttgaatca aaaatctatg cttccgaagt
    16081 cgacaagggc ggggcatata ttatttttcc gtatgatata agaaaagaat tcggtgctgg
    16141 aagagttaaa gtccaagcaa cttttgacaa tatgccgtat gaaggcagta tcgtgaatat
    16201 gggcgtgaaa aatccagatg gttcagtctg ctacatcctt ggactacgaa aagatattcg
    16261 taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt acagtgaagc aaaaataaaa
    16321 gaggttgtga caaaagacgt gtcacaacct cttgataaac ggtgttcaaa agccagtttc
    16381 tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca tgtttacaaa atttttctta
    16441 ctactcgaaa cgaagcggct ttatcacaac ctcttgataa acggcgttca atcagctgtc
    16501 cctcggtatt agctcaaaaa cggcaaaata tgagaagcta tttttccatt tttttctctc
    16561 atactcaggt cataacgctg atttcacaac ctcttctttc ataatgtttt ttcaccatct
    16621 ttataataaa ctggttcttt gatatggaaa tctatttttt ccaaccaatc taaaatcttg
    16681 taacctaact cttctgacat cggaaaagag gcgttgattt gtgcattgga gatatggtca
    16741 acatggatac cggaagtgat cgtacaactt cccggaacgg cagttttgat gatcacagct
    16801 attttttcgg ctaagacatc gtctttatgg aaccgaccat cgtggctagg gaaacgtaca
    16861 gtttgcatct ctgtatcacg agaaaatgta gtgactgttc cgatatgtgg tgtatctccg
    16921 cctgttaaag tgatcaataa gtctttaccg attactttaa cagcagcttg gatcgtgaaa
    16981 cctagtttgg ttacttcaac tatcgtttct ttttccatat taatcaccct gccaacgttt
    17041 tgaatgctca tttacaattc caaattgatc caaaagtttc attgtatggt gattaacaag
    17101 atcttggatc gtttgaggct gattataaaa agcaggaatt ggtggtatga tttgcacacc
    17161 tagtcgtgct aatttagtca agttttccaa gtgaatggca tttaatggcg tttcacgagg
    17221 aacaatgact aactttcgtt gctccttcat tgttacatca gcagcacgtg caatcaggtt
    17281 gtcgcctaaa ccaacagaga tacctgcgac tgttttcata cttgccggta cgatgatcat
    17341 tccatcggtc aaaaatgatc cgctggcgac agcagcacct aaatctttgt ttgaataatg
    17401 gtaatcaaac aggctttcga attcagttag tgataagtcg gattccagct ttaagttctg
    17461 ttttgcccat ggactgatga ttccgtgtgt ttctatcatc ggatattctt ttagtttctt
    17521 cactaggttg atggcataaa tggtgcctga agcgccactt acaccaatca cgattttttt
    17581 cttccgttgt gccaattgtt ccattttccg cactcctttt tatgatgtca aaacagttgc
    17641 agatcattca ttatttcaaa tatttttccc aatcttctac ttcttggaat tttgcacgta
    17701 caaaatgttc tttcatatcg aaaggaactg taccgtcaaa gatagttttt gcactcatcc
    17761 cacggaaacg aatagagcca ggattatatt ctggtaattc tgatgggtcc aatggatggt
    17821 tcctcattcc tgggatcacg atgatatctt tatctccttg gaaacgagtg ttgaaagtcc
    17881 aaactacatc gttcatgtcg aaaatatcaa catcttcatc gactaaaatg actgtcttca
    17941 attctttgaa ggctgataaa gcaagaatag ctgcttgacg ctgaatacct tcatccgctt
    18001 cgttttcttt acggatttgc atgatggtca tcaatttacc accacctgca ggaggattgt
    18061 agacatttac tacttttcct ggaatagctt tatcgaccaa ggcaagaata cttgcttctg
    18121 taggaatacc ggccatagat acgtgttctt cactgggacc aatcgttgtt tgcatgattg
    18181 gattgtcttt tcggtgagta actgctttga ctttcactac ttgtacagct ggattagcat
    18241 ccccagtata gccaggaaat tctggcattg cttttcctga gttcgtgttg atatcttctt
    18301 gcattgtttc atttggcatg atttctgctt caatgatgaa ttcagcacga gcaataccca
    18361 cttcatcgac tgctatacca tcaacgattt gaataggttc gttacgcaaa gctcctgcac
    18421 accatagttc attgtagcca agtggagtgg taggtggttc gaaagtcgtg ccaatggcaa
    18481 ttgctggatc taaaccaata ttgatagtga taggcattgg tttattcaag gcttcgaatt
    18541 gtttttggaa gtgaccaatg tggcgtccgc caggcatgat gtacataccg atcgtatctt
    18601 tatcttctag aaccatacga tggatcgtta catcgctcat tgttttttct ggatctgatc
    18661 ctaagactac acccattgtg atgtatggac cagcatcttc aggagtattt gtaggtgcag
    18721 gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag atgaacgact tcttgtgctg
    18781 gagcgtctgc tttgtctact ttcacaggtt tgattggatt ttctacagcg tctttcaaga
    18841 aacgaccaag tgttcgataa tcatggtgca aaatttcacc tacacgttta cgactagcca
    18901 ttgttccgat gttcacacgt gtgccgggga aacctttgat attgttgaaa gtcatagctg
    18961 gtccttcttg agtcggacgt tttactgtac cgccagcacc gatataacgg taaacaccag
    19021 ataattctgc ttcaggatcg atttcaatat ctgtttcgtg atattgacct ggaagtgttt
    19081 ttagttcttc gatgactttt cttaaatcat acggttgatc tgtcatgaga gattcctcct
    19141 tagttgtctt tacctatcta tagtaaaagg tgaaaaagag gaaaacaatt caacttgttc
    19201 ggtaaagtat tctacaatgg aatggatgcg tttttttgat aatagaataa actatgatat
    19261 acttatcact aaaagaaagc gcatacttag tgttggagga aggaaagtga aaaaaatgaa
    19321 ggagtcattt cctaaagcag taaaagtgga aaatatagcg aatattttga aagttacatt
    19381 tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca gaagagataa ccgatgcctt
    19441 gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt ctcgcacttt ctcgtaatat
    19501 gtggattggg actgaagtaa cgattgaagc agatggaaca gtattcataa atggaaaaga
    19561 caggtatacg ccagaagagt tgtggtacaa aggaaagaaa agtataccag aattataaaa
    19621 aataattaaa aaaaacagcg cgaaacaagg aacatattac ttcctcgttt cttgctgttt
    19681 tctactattt cagcgctctt atgactcaaa tctaaatgtg gttgtcacaa ccttattctg
    19741 cacgcaaagc gatagcggcg tcttttttcg ctgccattcg tgcaggaaga tgaccgccaa
    19801 gcatcgtcaa tatagtagag acgatcacta gaataatcgc atggacagga tttagttgag
    19861 caacattttt tagatccgtc atgttgtata agatgctatt gatagggaac gtagctagcc
    19921 atgcaataaa cacacctaat atcccagaag aaatacctaa aatacacgtt tctgcgtcaa
    19981 atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt tagtacaccg atttcttttg
    20041 tccgttcaat cacagatgta taagtaatga tactgatcat gatcatactt gttactaaag
    20101 agatccctgc aaaagcaata aggacataag tgatagcatc catcaatccg ccagtcagtt
    20161 cagtcatcgt tccagctaaa tcagtataaa tgattttatc ttcatctgat ttgcctttat
    20221 tgtaatcatc tagataatcc aagatctttt ctttgtcttc aaaattgttc ggataaatca
    20281 tgatacttga aggcaagctg tctccaccta aataactaag caatgtttgt ttggcatttt
    20341 cgtcaacttt ttcagtagtt aagacattta caccactgtc tctttgagct ttgacgattt
    20401 ctgattcttt gttttcgttg acgatttgag tcgttaattg atcgctataa gcgattcctg
    20461 gagataagag attcattgta gaagaagatt tcacgcgtag gatccccgag atcttcaatt
    20521 catcagaagc attctgatac atcgcatcat aatctgtatt agggataaaa ttcccagttg
    20581 gaagttttgt atagtaagta ttgttgttta ctaatttgaa tgttgtacca acgatatcat
    20641 caaaatcaag tttttcgtct tctttcacat caaatccaag attcttcaat gcgttgatat
    20701 tagtattatt attgccgtct acgatcagca cgacatctgt tgctgaggct ggataacttc
    20761 cggcaagtaa agaataattg tcttttaaaa agttttcttt gctagtgtca agctgtgtag
    20821 ggaaacttga cactcccaca cctgtcattg ctgacatcgt actagataaa gaaagagatt
    20881 ccgtatcagg attttgattc gagaagctga caggctgtac tttgccattt acatcacgta
    20941 aaagattgat tcctgtagta cgggtaaatc cgatattatt acttaagtta ggatcgatgt
    21001 ctgttacgta atcgacgtat gtttgatcaa tcttattcgt gtgctgtgct ttatcttctt
    21061 cgctgacttt tgcagtaatc gttttgctgt ctggataatc cgctttgctt gcacccaatc
    21121 cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat agtaatcgga aatttcgcca
    21181 tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc agaagaaagg gacagaacga
    21241 tcgcgattcc gatgatccca atactggaag caaaggaggt caagaaagta cgtccttttt
    21301 ttgttcggat attgttgaag gaaagcttta acgctgtcca aaaactcatc tttgttctgc
    21361 gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg atgggaatct gttaagatct
    21421 taccatcaga aaattcaatg atccgatctg cgtattgatg ggccagttca ggattatgtg
    21481 tgaccatgat cactaatttt tctttcgata actcttggat caattccatg atctggatac
    21541 ttgtttctgt atctaacgct ccggttggtt catcgcacaa aaggatatct gggtcattgg
    21601 ctagtgctcg agcgatggcc actcgctgca tttgtccacc agataactga tttggctttt
    21661 tatgcatatg atctgtcaag ccgactcggt gcaatgcatc ttctgcccgt tttcgttttt
    21721 cgtctttaga aacaccactt aatgtcatcc caagttcaac gttttcaata atgcctagat
    21781 gaccaatcaa gttgtaactt tgaaaaatga aaccgataga gttgtttcgg taggcatccc
    21841 agtcactgtc cttaaagtct ttagtagact ttccgttgat taccatatca ccggaatcat
    21901 aattatctag tccaccaatc acattcagca tagtcgtttt accagaacca ctaggaccta
    21961 aaatcgctac gaattctttt tgtctgaaag cgacagacac accatccaat gctttggtgg
    22021 tggtttcgcc gactttataa tattttttta tatcttttaa ttgaagcata atcacccttc
    22081 tcccgtattg aaatattcgg tatactaata agtatatcag aacaatgatc gtagtatacg
    22141 aaataaatgt taactgaaga tgaactacaa gaaaaaatct agaaattttt caaaaaacag
    22201 agggagatca atgaatcgta ttttagtagt agaagatgat ccgcaattac agttgcttta
    22261 ccgttctgtg ttggaacgag cgggcttttc agtcatcacc gcattgaatg gagaggatgc
    22321 gctgaaaaaa ctagaagcga cacgtataga attaatcatt acagatatca tgatgccgaa
    22381 aatggatggc tatgacttgc tagactcatt acgtagcacc agatttgaca cacctgttct
    22441 aatgattacg gcaaaggccg attttgagga taagaaacgc gggttcaaat taggagcaga
    22501 tgattatatg acaaaaccca tcgatgtcaa tgagatgatc ctacgtgtgg aagcattgct
    22561 tcgacgttca aagatcaatt acagcagtag cttgataatc ggacaaacaa aattagagct
    22621 agatgcttat acagtgttac aagatcagca ttcgttgatt ctgcctcaaa aagaatttct
    22681 gcttctgtat aaattactgt cttatcccaa caagatcttc actcgccaac agttgatgga
    22741 tgatatttgg ggattagata cgaatacaga agaacgaacg atcgatgtac atatcaaacg
    22801 tttaaggacc cgttttgaaa agacggaaga ttttcaaatc atcacagtac ggggactagg
    22861 ctataaggcg gttatttaaa gatgaaaaaa aagctatttt cacaattatg gatctatttt
    22921 gctagtattg tcttcatttc gatcttagtg acactgctgt gttttctcgg ttttattttc
    22981 ttattgtctc gacattctat ctcgcctgct gaatcggctg gaaaaccaac gttatacccg
    23041 ttattcgtct ttgcggggtt cagtatggtc gtgggtacag gaatatcgat ttttgttggg
    23101 cggcgtatcc tgcatccaat cagtgcgcta gggacaaata tgagtctggt agcaacggga
    23161 gatttttcga ttcgaatgga tgaacagcag aaagtagcag aagtacagca actgtataaa
    23221 gatttcaacg ttatggtcca agaactcaat agtatcgaga cactgaggaa tgactttgtg
    23281 tccagtgtat cccatgaatt caaaacacca ctagcaacaa tccaaggata tgttcaattg
    23341 ttacaagccc caaatctctc tgacgaagaa cgccaaatat ttttatatcg gattatcgaa
    23401 agtatcacgc agctttcaca attaacagag aacatcttaa aattgaacaa actggaaaac
    23461 cagcgtatcc aactggaaaa gaaagagtat cgattagatg aacaaatcag agaagtaatc
    23521 gtttttttgc agcctaaatg ggagaaagaa cagctagaat tggatatcga acttgctgca
    23581 gtaaattaca caggcaatga agagtttttg taccaagtgt ggctgaatat catggataac
    23641 gccataaagt acaatcaaat caatggacag attcatatca aactatttga gacagccaca
    23701 gagattggac tggaagtgac cgattcaggt gtaggaatga atgaagagac cagagatcga
    23761 atgtttgaaa aattttatca aggagataca agtcgtcaaa tttctgggaa tgggctagga
    23821 ctttcactgg ttaaaaaaat tctagaactg catgatggac gtattgatta tagcagtatc
    23881 gaaggagtag gaacgacagc aatgatacgg ttaagtaaac aataggaaaa agaataaaac
    23941 ccctaaaaat tctattgaag gtttttaggg gtttttagtc gtttttttgt gattagggaa
    24001 atgttcttac ggttcttttg gcataaaaat cgctgctttc ttattgaact acgagaaatg
    24061 ttagaggtgt cattgaaaca aggacatgat gcaagaaaga gagcgaaagt catgcgtcgc
    24121 tctctttttt tgggtggata gatggacaga aaatgtaggg cttagttttc ttcttgattt
    24181 aaatactccc aaaatgtatc tgaagccaaa gaatgctcac cgactttacg agtaaatgca
    24241 agataactct ctgttgttgg agaaataggg agaatcacag tattttcttt taatagattc
    24301 ttgactgttt tttccataac aatagcaatc cccatgtcat ttgcaatcat attgatgatc
    24361 aagtcgattc ttgctccttt ataagagatt tttggttcaa atccagcttc gcgacacaga
    24421 tctagtacag gttgatacaa gttcgtagtg gggccaagga gcaaaaagtt ctcctttttc
    24481 agttcgctta gatccaattt ctttttacta gctaaaggat gatttttagg tagaactgct
    24541 acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac ttggtggggc ctggaaattt
    24601 ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat tgtaagttaa ttcgacactt
    24661 tccacttctt tcaactggac tgtaaattca ggatggcgtt tcaaaaactc ggtgattttg
    24721 gaaaaacctt tataattgat cattgtcggt atcgttaaga tatttaatga aagattgttg
    24781 tctttcgtat aggtttcaag cacttcttgc atagcatggt attgttctac gacatttttt
    24841 acatatggga gcaataagct tccagcttcg gtcaattcga tttttctatg ggcgcgttcg
    24901 aataatttga cgtctaattc tgtttctaac gaaagaatct gtttagaaat atttccttgg
    24961 gtggtgaatt gtcgatcagc agttttggtg aaattcattg tttcggctaa atcaagaaaa
    25021 acttcgaatt tatggatatc catcatattc ctcctactta ttccaataaa gaatacttta
    25081 acacaaaaaa tgaattggtg taatcttctc tgtctgttac aatctttctt gcagcgaaag
    25141 caataattac agaaattttt ctgatttttt tcgcttcgta gaaaaagaga ggagtttttt
    25201 gatgccaact ggagttttga tcaatgtagg ttccgttttg cttggcggac taattggcgg
    25261 attagtagga aataaattaa gtgaacattt taaagcacag ctgacgatgg tttttggtgt
    25321 gtgttcgatg gggatgggaa tctattccat tgcgccgatg aaaaatatgc cggctgttat
    25381 ttttgcttta gtgataggaa cagcgattgg tctgattgtt catttaggta atgggataaa
    25441 taagggggct gcgctgatgc aagtacctat ttctaaaatc ttccctagtg aaaagcttgg
    25501 tatgacccac gatgaattta tcagcacatt agtgacggtc attgttctat tttgtgcaag
    25561 tggtacgggg atctatggaa gtttagattc tgggatgaca ggagacagta cgattttgat
    25621 ttctaaatct gtccttgatt ttttcacagc agctattttt gcgtgtaatt tagggtatgt
    25681 cgtttctgtg atagcaattc cccaattcat tattttttat attttgttct tattggcaaa
    25741 atttatttat ccattgacta caccagacat gatcttagat tttaaagctt gcggcggatt
    25801 cttgatggtc gctaccggtt ttagaatgat caacgtgaaa atgttcccag tagctgacat
    25861 gatacctgca atgatcgtta tcatgccact gagctggatg tggacaaatt ggattatgcc
    25921 actgttataa taaaaatcaa agagaagcca ttttttgctg actaaaaagg atcaaatctt
    25981 ttagtcagtt tttttgtatt tgacaaactg attttgaaac tatattatat gttgtataat
    26041 atagattgaa ataaaatatt acacttgaga atagaaggtg acgtaatgca catccagatt
    26101 ccaactgttc ttcttgatgg tactgtgcta gcagcattaa gcaaggaaga tatgtatggg
    26161 tatgccttaa caaaaatcgt acaagaaagt ctctctgtca gtgaatctac actatatccg
    26221 gttttgagaa gattgaaaaa agaaggatat cttgagacct atgatgaacc ttttgaaggc
    26281 agaaaccgtc gatattactc gttgacacca atgggaagtc gtcatttaga agaaattatt
    26341 gatgaatgga ttgtttttcg agattcttta gaccggttat tggaggagag cacaaatgaa
    26401 tgaatatttg aatgaagttg tggatcatct tcaagaaatt cctgaagacg atcgttttga
    26461 tttgatccag tattatgagg aatatttttt agattctgga aaaacattag atcagatcat
    26521 tgatgaatat ggtacaccaa agcagtttgc cttaaaatta aaaatcagtt acttttcaga
    26581 agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa agacaaattc gcctgatttg
    26641 gttaatcatt atcggtttat ttgcttctcc tgtcctcatt ccactagcac tggggtttat
    26701 attggttatt gggggactat tggttgcttt actagcgctc atttttagta tctatgtttt
    26761 atgtatcagt atcttaggtg ttggcctatt catgatcatc tcaggaatca gtgtgtttgg
    26821 acaatcgatt ctgagtggat tattctttat cggtgtcggt ctgttcgcca caggaagcgc
    26881 agtatttatt tcgccactgt tgctgaaagg aacaaaaggg cttttttatg tcatgatgaa
    26941 ctttgttaaa tggatgggac gacgttttat tacaaaaaga gatctgcatt cagcgggcgt
    27001 ttgaaggagg acacataaat tgagattgaa acattttttg attaccggta ttggtctgat
    27061 gcttactggt ggcctcctag caggtgcaac ttatacatta ggcgctcaaa agagtctgac
    27121 ttgggataat ggtcctcggt tgatcgatat ggttactgag acaaaaaaaa tcaaggatgc
    27181 aaacaaaatc gtcatccatg cagaaaatca aacagtttcg gtcatacgcg gattggattt
    27241 tgagataaaa acgacttatg ataagacaga aaagccagaa ataaaggaga aaaatggcgt
    27301 gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc attagtttag aagacacaag
    27361 tgcacaaatc actattgttg tccctgcaga tcatgaatta tctgaattga caatcgaagg
    27421 gaataatagt gcaattgata tcaacgaagt atcaatcaaa aaagtgaata ctaagacaca
    27481 tgatggctgg gtatcgttca atgaatttac aggaggaaca ataactgcga aagatgaatt
    27541 aggctcactt cgattagatg aggccaatgt ggataacttg atcgtcacag gtgaaaacag
    27601 caacttccat ctagaagaca gtttgatcag caaagaagga aaagtcatcc taaacggtgg
    27661 caatttatct ttgcatgaga tggaaaacag tggctatact atccaaacgt ctggtgaatc
    27721 ttatattgaa aaagactacc agccaataac aaatactttg ttagaacaag gacagaaaaa
    27781 gctaaagatc actgctaaag acacatcgat caatattacg acggatcaag aggacaatga
    27841 aacagattga tcctgtactc aactggatat tttaagaaaa aacagcaaag atccttccaa
    27901 tctttgctgt ttttcatcgc ttaacgcaca taactatagt aaaataagaa agaaaatatc
    27961 taaaaagaaa acagggtgaa agagtatatg aaggtagaag aaggtcaaag agaaaagtta
    28021 aaaacagaaa tggaaagatt acatacttat atcactcaac taagccaaac cttctatgat
    28081 ccagacaaag aaaaagtgat ggttaattac ccgaacaatt cagaaggtcg ccaattagag
    28141 caagtgtacc atgaagtttt caaacattta ttgacggtga agaaagagct ggattattat
    28201 tctttaccga ttatcgatac aggaatattg aagtatgatg ggaagaagga acgatttatc
    28261 tttaaatctg ttcgggaaga tttgcctctt tcagcaggga tggatctaga ggtcttggta
    28321 gaagactatt ttacagaaga aaagcactgg gttcgaacaa aattggatta tttaccgcag
    28381 gcagccggag ggacacaggc aaggggttgg tacatcactg aagacaaaga gttagaacta
    28441 gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag taacattaga tatttattga
    28501 aggataaagg agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta
    28561 tgcagggatt gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg
    28621 tatgtggcaa gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt
    28681 attgttgtta agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt
    28741 tggacaatat ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca
    28801 aaaaatgact gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga
    28861 tttggatgga aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc
    28921 aaaggcatta ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc
    28981 cattcggcat atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct
    29041 tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc
    29101 agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat
    29161 cgctagtgcc atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat
    29221 tgttgaagga gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga
    29281 aatcaatttc accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat
    29341 tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca
    29401 tactttttgt aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt
    29461 tagttgtttt aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga
    29521 attgttcgct tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa
    29581 aacttctgat cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag
    29641 attacgtagt tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa
    29701 cagtttcaaa atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct
    29761 atacgaaaga gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc
    29821 gtaaaggagc atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag
    29881 aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa
    29941 agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga
    30001 gaaaaagacg caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg
    30061 gatcatgggc gcttctggtt ctggtaagtc gacacttttg aatattttat ccacattaga
    30121 taaaccaaca gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa
    30181 tcaactagca gattttcgtg cgaatgagat tggctttatt ttccaagatt tcaatttatt
    30241 agaaagcttg acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc
    30301 aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt
    30361 ggaaagctat ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc
    30421 attgatcaca caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa
    30481 aagtgcccgg gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat
    30541 tcttcttgtg acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa
    30601 ggatggcggt atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg
    30661 agaaatacta tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga
    30721 aattatcact tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat
    30781 tagttatggc atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt
    30841 tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac
    30901 tagggatcat cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc
    30961 aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg cagaaaaatc gcacagatga
    31021 ttttttctga aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg
    31081 taggattgac aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc
    31141 attttacacc ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt
    31201 ttttattagc tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga
    31261 ttcgtgcgga tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag
    31321 tagtactggg aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct
    31381 atcaagtctt tggtataggg gtagccttag ttacgatcat attaggaact tatttcattt
    31441 tccattctgt gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga
    31501 aaaaattgaa caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa
    31561 tgctctcaat ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag
    31621 gttttaaaaa tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga
    31681 ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt
    31741 cttatactca aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc
    31801 aaccattgat catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag
    31861 ttcctgctaa aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc
    31921 cggaacagca agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact
    31981 tgccacaatc cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa
    32041 tcgaaacact tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca
    32101 gcaaccagaa atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta
    32161 tgggattctt cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga
    32221 tcttgtcagg aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac
    32281 gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag
    32341 gaatcttagg tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa
    32401 atccatacaa agatatttgg gttccgttta cgatcttctt cgtattgtat ttcatctact
    32461 atgtactgac gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct
    32521 gtcctgcatc aagtaataag aacagccaag caaaaatagc tcctcatatt tttcgtttgg
    32581 ttgggcttat taccgcagat gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt
    32641 gacaatattt ttgtcacaac ctttttttca cggtctttta ttagaatata gtaaattttt
    32701 tctcgttact cacggaataa gctgttaaga aaacggtttc catgtatact ggaatcagtg
    32761 caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa
    32821 actatcactt gattacgatc acgaatcaaa atgggacatc tttgtctgtc agtgatcttg
    32881 gggcacgggt tgttcatttt caaacaataa tcgataatga aaaacgagag ttgatcttag
    32941 gttttgatga agcaaaagaa tatctagaaa aagaccctta tatcggtgct tcaatcggac
    33001 gtatggctgg acggattacg aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa
    33061 aaacagaccc ggacactggt cattgtttac atggtggtgc acctagcttt gaagccaagc
    33121 agtggcatta tacagtcatc aatggtgaaa acgaagcatc ggtcattttc tatcagacta
    33181 gcccagatat gggaaacgga tttcctggaa ctttagatgt agaagtacgg tatacgctga
    33241 cgaacgatga tacatggcga gtgacggtcc aagggatcag tgaccgcaaa acagtattca
    33301 atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc agcacagtca atcgacgaac
    33361 atatactttg gttgaatagc cgattatttg ctacgctcaa taaagataca acgcctacag
    33421 gagaagtagt ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa aaattaggtg
    33481 aggtctttgc cagtgatttt cgccaatcaa tgatagtggg cggcatcgat catccatttt
    33541 tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt gacaagccca gatgaaaaag
    33601 tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat ttttacagca aattttggag
    33661 aagatggacc ggaaatgcgc gggaataaat tagtaaatca tggcggtatc acatttgaga
    33721 ctcaagaact gccaggagcg gagcgacttg aaagttttgg agatgtcact ctggaaccta
    33781 accaagtacg cgagtttgta acggaataca aaataa
//
LOCUS       DANJJH010000021        33651 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000021 DANJJH010000000
VERSION     DANJJH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..750
                     /locus_tag="PWS53_000947"
     CDS             <1..750
                     /locus_tag="PWS53_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0871208.1"
                     /translation="EILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELT
                     NWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLD
                     KYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSG
                     EVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHS
                     DISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(838..1230)
                     /gene="rpsI"
                     /locus_tag="PWS53_000948"
     CDS             complement(838..1230)
                     /gene="rpsI"
                     /locus_tag="PWS53_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0871209.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1246..1689)
                     /gene="rplM"
                     /locus_tag="PWS53_000949"
     CDS             complement(1246..1689)
                     /gene="rplM"
                     /locus_tag="PWS53_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL0871210.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2208..2675)
                     /locus_tag="PWS53_000950"
     CDS             complement(2208..2675)
                     /locus_tag="PWS53_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL0871211.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2795..4333)
                     /locus_tag="PWS53_000951"
     CDS             complement(2795..4333)
                     /locus_tag="PWS53_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL0871212.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4413..5099
                     /locus_tag="PWS53_000952"
     CDS             4413..5099
                     /locus_tag="PWS53_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL0871213.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5300..5827)
                     /gene="msrA"
                     /locus_tag="PWS53_000953"
     CDS             complement(5300..5827)
                     /gene="msrA"
                     /locus_tag="PWS53_000953"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0871214.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5911..6588)
                     /gene="pgmB"
                     /locus_tag="PWS53_000954"
     CDS             complement(5911..6588)
                     /gene="pgmB"
                     /locus_tag="PWS53_000954"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0871215.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6585..7715)
                     /locus_tag="PWS53_000955"
     CDS             complement(6585..7715)
                     /locus_tag="PWS53_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0871216.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7715..9898)
                     /locus_tag="PWS53_000956"
     CDS             complement(7715..9898)
                     /locus_tag="PWS53_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0871217.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9895..10920)
                     /locus_tag="PWS53_000957"
     CDS             complement(9895..10920)
                     /locus_tag="PWS53_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0871218.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10965..11792)
                     /locus_tag="PWS53_000958"
     CDS             complement(10965..11792)
                     /locus_tag="PWS53_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0871219.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11789..12856)
                     /locus_tag="PWS53_000959"
     CDS             complement(11789..12856)
                     /locus_tag="PWS53_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL0871220.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12882..13718)
                     /locus_tag="PWS53_000960"
     CDS             complement(12882..13718)
                     /locus_tag="PWS53_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871221.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13715..14584)
                     /locus_tag="PWS53_000961"
     CDS             complement(13715..14584)
                     /locus_tag="PWS53_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0871222.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14654..15937)
                     /locus_tag="PWS53_000962"
     CDS             complement(14654..15937)
                     /locus_tag="PWS53_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL0871223.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15951..17636)
                     /locus_tag="PWS53_000963"
     CDS             complement(15951..17636)
                     /locus_tag="PWS53_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL0871224.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17911..18936
                     /locus_tag="PWS53_000964"
     CDS             17911..18936
                     /locus_tag="PWS53_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871225.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19531..23205)
                     /gene="rpoC"
                     /locus_tag="PWS53_000965"
     CDS             complement(19531..23205)
                     /gene="rpoC"
                     /locus_tag="PWS53_000965"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL0871226.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23263..26880)
                     /gene="rpoB"
                     /locus_tag="PWS53_000966"
     CDS             complement(23263..26880)
                     /gene="rpoB"
                     /locus_tag="PWS53_000966"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0871227.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27608..28576
                     /locus_tag="PWS53_000967"
     CDS             27608..28576
                     /locus_tag="PWS53_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0871228.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28594..29499
                     /locus_tag="PWS53_000968"
     CDS             28594..29499
                     /locus_tag="PWS53_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871229.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29496..30302
                     /locus_tag="PWS53_000969"
     CDS             29496..30302
                     /locus_tag="PWS53_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871230.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30438..31427
                     /locus_tag="PWS53_000970"
     CDS             30438..31427
                     /locus_tag="PWS53_000970"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL0871231.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31744..32268)
                     /locus_tag="PWS53_000971"
     CDS             complement(31744..32268)
                     /locus_tag="PWS53_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL0871232.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32555..>33651
                     /gene="scm"
                     /locus_tag="PWS53_000972"
     CDS             32555..>33651
                     /gene="scm"
                     /locus_tag="PWS53_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0871233.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTST"
BASE COUNT         9526 a         7197 c         6315 g        10613 t
ORIGIN      
        1 gaaatactaa attatatcga atttaatcga actactaaaa ttattcaaag tatcactgca
       61 cctaaaaaaa atgctttaga agaaaaaaga atacaagaag gtaagcaagc actatcatca
      121 aaagaactta ctaattggat agaagcagta aatgatgatt ttaataatca tttacttact
      181 tttcatgatt acacactttt tatgattacc ctgtatcttg gtgatagaaa aagtgagact
      241 tacgctttgc aatggaaata tattgatttt gaaaaacaaa cggtccgtct taaacatact
      301 ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa aagataccgt cattcaagta
      361 ccagaagttg taatgacatt gctaagtgaa tggaaatcgg tacaagctga tcaattgttg
      421 aaattaaaaa ttaagcaaac gttagatcag tatctcttta cttatactaa accatctgga
      481 gaagtaaatt gtcctgttca tgccgattac ctaaactatc gaatcaattc aattaaaaga
      541 aggcatcctg acttagttca tttatctcca cacaaactcc gtcatactta tgcaactata
      601 gctagacaag gtggagcaga tatgaatcaa atttcaaatg ctttaacaca ctcagatatt
      661 tctacaacaa agatatatgt gaacacacca gatatagttg ataaggctgt ttttgaagct
      721 tttcagagag gattgaataa gtgtgattga tatgtgttct taaatgtgtt cttaaaaaca
      781 aaagaagcac tttccaaata attgaaaagt gccagaaacg ttgatatata aaggatatta
      841 acgttttgag aattgtgaag ctttacgtgc tttcttaaga cctggttttt tacgttcgac
      901 catacgtgcg tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc
      961 aacttctagc aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc
     1021 tccgccgttt acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg
     1081 ttgattgatt acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt
     1141 tacagtaatt ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg
     1201 gcctgtgccg atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt
     1261 gatgtctaaa acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt
     1321 caattttgta aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt
     1381 ttcgatcaaa cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc
     1441 tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt
     1501 cactttgtct gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt
     1561 tggtttattt ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac
     1621 atcagttgcg tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt
     1681 tgtacgcacg ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc
     1741 cggggctctt tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg
     1801 tcaacgtttt tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt
     1861 atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt
     1921 gtcacaaccc cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     1981 caatatccat aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga
     2041 acggcaggct tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct
     2101 caaaaaacgg caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa
     2161 aacgctgatt tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc
     2221 tctggcgctt tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg
     2281 aattcgataa agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct
     2341 ttttggtaaa gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga
     2401 atagtacgat cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc
     2461 aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga
     2521 gcgttcaaat catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct
     2581 ctcatgtacc aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat
     2641 tgatttaata cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt
     2701 acacttaaaa taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac
     2761 gattttcagt tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt
     2821 ctcgcagttg agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata
     2881 atggatagat tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca
     2941 attctatcaa ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta
     3001 aaatcacagc tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca
     3061 gtacatcagc catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga
     3121 tctctgtgag ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga
     3181 tcccttctag aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa
     3241 aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa
     3301 aaatcagacc attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg
     3361 gattttctcc tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc
     3421 aatcaaagat gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca
     3481 gaacataaca aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa
     3541 gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta
     3601 gattagaaag cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta
     3661 gttctgctgc tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg
     3721 gcaattgctc tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat
     3781 ggatatcaaa atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca
     3841 atctataaaa gatatagtct ttgataccga caaaataagc agtttttttg aataattcag
     3901 gttggctgtt ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc
     3961 cagtctgccg ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg
     4021 ctgctcggtt atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca
     4081 atatcaggcc atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca
     4141 actgtttaat tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt
     4201 gttctgccat cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt
     4261 cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga
     4321 ttgaaacgtt cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt
     4381 gcgtatcttg gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct
     4441 gtatcggatc atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt
     4501 atcctagatc tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga
     4561 ttctctcgat catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct
     4621 actatttgtt ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt
     4681 ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg
     4741 atttatttta cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga
     4801 ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag
     4861 gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc
     4921 tttcttggag tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc
     4981 tgtttggaat atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc
     5041 tgcctttcat tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa
     5101 aaaaggttgt gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc
     5161 cttgtgactt ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag
     5221 ctgaccctca gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat
     5281 aagtagcttt ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac
     5341 gtccagaacc ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat
     5401 ggtaatcttc tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat
     5461 cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt
     5521 catcattata gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg
     5581 cgtcagtcgg atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt
     5641 ctggatcata ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt
     5701 cataagttgg atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg
     5761 gctgctcttc aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt
     5821 ttgtcatata aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg
     5881 ttttgcttgt gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg
     5941 ccaaaacgaa cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg
     6001 aagatttatg ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca
     6061 accagcttct gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc
     6121 tcctttgaga aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag
     6181 gacatcaaaa taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc
     6241 tgatcccaat ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc
     6301 aggcaaaacg gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg
     6361 ctcagcaaag gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag
     6421 caatcgctct agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt
     6481 gaatggaatg gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt
     6541 gtctacaaga acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct
     6601 tgaaaatgaa cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca
     6661 gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc
     6721 gaaatgccaa catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc
     6781 acaggaacgc cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt
     6841 tgtccatcta gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg
     6901 aactccagta aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct
     6961 aacagtcctg cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg
     7021 gcaaatctca tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc
     7081 aagccttttt gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta
     7141 acatcagtaa tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca
     7201 actgtagcaa ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg
     7261 taaaaagcat aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg
     7321 ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct
     7381 actcctcgcg tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg
     7441 acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg
     7501 tttttgccaa atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct
     7561 aatgatgaaa ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga
     7621 acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt
     7681 tctttgaaag aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca
     7741 gttctttttc cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct
     7801 ctttttttat tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa
     7861 taaaaggaaa agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc
     7921 cattcgttac tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg
     7981 aagcagcatg aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg
     8041 cttcttggaa taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt
     8101 gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct
     8161 tagaaacgat tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg
     8221 cttgcttgct gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact
     8281 ggctgccagg acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt
     8341 catcttgcgg aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat
     8401 tttgacattc tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat
     8461 tataatgcgc cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg
     8521 gaccaatgac tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa
     8581 aagctgctgt ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg
     8641 ttgcttggac atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg
     8701 cagctgaagc aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg
     8761 tctcttcctg accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat
     8821 ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg
     8881 ctacttcagg aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa
     8941 catgcccttt ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat
     9001 tgcggggagt cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag
     9061 aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg
     9121 actgctctaa aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa
     9181 acgggtctgt ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg
     9241 tattttttct gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata
     9301 cacctgcttt attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt
     9361 aggatgcact gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg
     9421 taccaaaatt ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg
     9481 tcaacggctt gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac
     9541 gacaagtaat gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa
     9601 gctctcccat ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag
     9661 aaaaatgttc accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact
     9721 cggctgattc tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt
     9781 gattccgata gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca
     9841 tctgactcgc atacttattg atatactcta aatcaaactg tttttcttct actttcataa
     9901 ggtaataact tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac
     9961 atatccttgc cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa
    10021 atgagccata cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag
    10081 ctttccatct acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc
    10141 acaaaaagcc acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg
    10201 gatgatcttg ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt
    10261 gcttggatcc catttcccga atttccctga attttttttc gtaccaattt tttggaagct
    10321 gtacgcccga acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat
    10381 gtgaatgcca taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc
    10441 ccagcctgga acaccagacc gacgaagcgc attcgcttct acgtaataaa tatccccgag
    10501 aacatcaaga tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata
    10561 agccaaaatc tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt
    10621 catagctggt ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg
    10681 ttcgaagtga aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag
    10741 catttgtgta actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc
    10801 tcgctggctg actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata
    10861 ataagcagca tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat
    10921 ttatttctct ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa
    10981 tcatttaaat gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa
    11041 attttcactt cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca
    11101 ttttgaagta gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag
    11161 cgatgattat ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt
    11221 gaaatatcat cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc
    11281 aattgattag agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg
    11341 tatcggttca aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa
    11401 taacgaagtg attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc
    11461 ggcggtaatc gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca
    11521 acctcgctta gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct
    11581 tcgataaagt caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct
    11641 gtttctttga ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt
    11701 tcgtattctt caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg
    11761 aaaaatgctt gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca
    11821 ttttgatact ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg
    11881 caaatggcac aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt
    11941 cccaacatgt gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac
    12001 tagatgctcg agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa
    12061 acccagctgg aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc
    12121 ctattaaaga agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc
    12181 cacgtttatc tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg
    12241 tgtaatcggc accgtgtttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa
    12301 cgatggtagt agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta
    12361 aaccgaagat tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag
    12421 cgaattgagc aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac
    12481 gtagtttata attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc
    12541 agactaactc tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga
    12601 caatcatatt tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc
    12661 ttggcaagaa taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc
    12721 taaagtcgac aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt
    12781 cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg
    12841 caactaattt tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct
    12901 tcgactaaat cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa
    12961 gcggtcacca atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat
    13021 gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat
    13081 aaataatcat tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata
    13141 gccaatggta taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct
    13201 gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta
    13261 ggaagtgttt gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga
    13321 ccggaaatga ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg
    13381 taaaagctag cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc
    13441 ccgctgatcc caatcactac tgcagttacg gaggctatca aggagatcac taaactattt
    13501 ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa
    13561 gtccattctt tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat
    13621 gatgtcatca gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc
    13681 gcgtaaaata agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg
    13741 agagcaagac gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc
    13801 caagatcatt gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat
    13861 tcgtcagtag atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca
    13921 cagttgctag tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat
    13981 ttgcaccgtc catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca
    14041 aaaatgaaat aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga
    14101 agctagcaac tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta
    14161 ctaacagatc attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca
    14221 aagaaataga tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc
    14281 tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa
    14341 ctaagaatgt gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa
    14401 tttcaagata attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat
    14461 cgaaaaaact gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa
    14521 gcatgcttgg tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat
    14581 tcattgaaac ctccccctga taatctgcca atagctatct acttttttcg aacaccattg
    14641 gcagaacttc ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt
    14701 cgtttccgac agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc
    14761 ccattttcgt gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac
    14821 tagcaatctc agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat
    14881 ccagttcgac tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact
    14941 tttccatgtt tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt
    15001 tctcgtctag accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt
    15061 tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca
    15121 aaatgtaagt tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt
    15181 ctgtcacatc attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg
    15241 cttctctcat cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc
    15301 catttgacaa agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca
    15361 tagcaattcc atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat
    15421 catcccaatt ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa
    15481 taccttgaac ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa
    15541 caaggttttt ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga
    15601 ctgccgtctg atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt
    15661 ccattacttc tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg
    15721 attcttctac cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa
    15781 ccagttcatt gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg
    15841 ttgtctgttg tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag
    15901 tagctagcag acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt
    15961 attttcagcc acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg
    16021 ataatttgtc cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat
    16081 tgattggaaa aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg
    16141 ataaaagaat ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct
    16201 ttttttcgaa tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct
    16261 gtctgcaact ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc
    16321 tctcctgttt tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa
    16381 attgccggca cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt
    16441 ctccattccg tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca
    16501 tatggactgg tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc
    16561 agctgttcca ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat
    16621 ccgataccgt catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg
    16681 atggttttta accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc
    16741 aaaggtggta gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa
    16801 ctgatattat ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct
    16861 tcatcagcga ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa
    16921 gaagttccta gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct
    16981 ttttctaagt aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa
    17041 ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg
    17101 tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta
    17161 tccagtggca aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga
    17221 aaccaagtac tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg
    17281 cttctcattt cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt
    17341 acaacggaga aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta
    17401 aatgtatctg ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc
    17461 tcttcaaaaa attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt
    17521 ttgcctgcct ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg
    17581 gttttctcag acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga
    17641 ttcccctttc ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac
    17701 tgtcaatagc atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta
    17761 tgtatcaata attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt
    17821 cctaaatttt caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa
    17881 aacgctccca aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa
    17941 aaagctaatg tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct
    18001 gataaagcaa aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa
    18061 attgcaagag gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact
    18121 tctcatacga cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt
    18181 tctgaacaaa ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta
    18241 gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct
    18301 acgatggatg aaacacttct ttttgaattg gatcaatata atattcctat tgtggtaatc
    18361 ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat
    18421 agttacgatt tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat
    18481 gctccgacag aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt
    18541 gatcaccacc tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat
    18601 agcatggcag caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct
    18661 accgatgcat taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata
    18721 cctgatgatc tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca
    18781 cagttgactc ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta
    18841 ttccaactaa tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt
    18901 actctcacag attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa
    18961 cactgaggat aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct
    19021 cccggtattt tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta
    19081 taatgtttgt gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg
    19141 tgtataagtt tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta
    19201 tttggggata cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt
    19261 ttattcgagg aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc
    19321 ccatcaattt attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc
    19381 ttggaagatg atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt
    19441 cccacttttc ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa
    19501 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
    19561 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
    19621 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
    19681 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
    19741 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
    19801 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
    19861 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
    19921 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
    19981 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
    20041 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
    20101 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
    20161 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
    20221 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
    20281 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
    20341 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
    20401 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
    20461 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
    20521 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
    20581 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
    20641 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
    20701 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
    20761 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
    20821 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
    20881 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
    20941 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
    21001 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
    21061 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
    21121 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
    21181 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
    21241 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt
    21301 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
    21361 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
    21421 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
    21481 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
    21541 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
    21601 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
    21661 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
    21721 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
    21781 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
    21841 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
    21901 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
    21961 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
    22021 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
    22081 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
    22141 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
    22201 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
    22261 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
    22321 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
    22381 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
    22441 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
    22501 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
    22561 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
    22621 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
    22681 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
    22741 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
    22801 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
    22861 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
    22921 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
    22981 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
    23041 acagaacaac ccttcacgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
    23101 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
    23161 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
    23221 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
    23281 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
    23341 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
    23401 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
    23461 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
    23521 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
    23581 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
    23641 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
    23701 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
    23761 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
    23821 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
    23881 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
    23941 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
    24001 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
    24061 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
    24121 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
    24181 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
    24241 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
    24301 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
    24361 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
    24421 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
    24481 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
    24541 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
    24601 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
    24661 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
    24721 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
    24781 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt
    24841 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
    24901 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
    24961 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
    25021 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
    25081 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
    25141 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
    25201 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
    25261 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
    25321 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
    25381 acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag
    25441 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
    25501 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
    25561 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
    25621 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
    25681 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
    25741 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
    25801 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
    25861 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
    25921 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
    25981 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
    26041 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
    26101 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
    26161 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
    26221 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
    26281 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
    26341 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
    26401 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
    26461 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
    26521 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
    26581 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
    26641 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
    26701 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
    26761 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
    26821 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
    26881 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
    26941 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
    27001 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
    27061 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
    27121 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
    27181 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
    27241 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
    27301 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
    27361 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
    27421 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
    27481 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
    27541 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
    27601 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
    27661 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
    27721 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
    27781 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
    27841 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
    27901 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
    27961 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
    28021 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
    28081 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
    28141 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
    28201 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
    28261 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
    28321 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
    28381 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
    28441 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
    28501 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
    28561 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
    28621 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
    28681 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
    28741 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
    28801 gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
    28861 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
    28921 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
    28981 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
    29041 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
    29101 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
    29161 atttctaatg gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct
    29221 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
    29281 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
    29341 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
    29401 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
    29461 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    29521 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    29581 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    29641 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    29701 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    29761 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    29821 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    29881 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    29941 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    30001 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    30061 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    30121 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    30181 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    30241 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    30301 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    30361 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    30421 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    30481 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    30541 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    30601 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    30661 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    30721 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    30781 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    30841 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    30901 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    30961 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    31021 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    31081 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    31141 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    31201 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    31261 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    31321 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    31381 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    31441 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    31501 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    31561 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    31621 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    31681 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    31741 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    31801 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    31861 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    31921 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    31981 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    32041 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    32101 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    32161 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    32221 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    32281 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    32341 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    32401 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    32461 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    32521 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    32581 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    32641 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    32701 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    32761 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    32821 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    32881 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    32941 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    33001 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    33061 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    33121 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    33181 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    33241 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    33301 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    33361 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    33421 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    33481 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    33541 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    33601 tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta c
//
LOCUS       DANJJH010000022        33378 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000022 DANJJH010000000
VERSION     DANJJH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..196)
                     /locus_tag="PWS53_000973"
     rRNA            complement(<1..196)
                     /locus_tag="PWS53_000973"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            937..1626
                     /locus_tag="PWS53_000974"
     CDS             937..1626
                     /locus_tag="PWS53_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871234.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1613..2821
                     /locus_tag="PWS53_000975"
     CDS             1613..2821
                     /locus_tag="PWS53_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0871235.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            3115..4386
                     /gene="serS"
                     /locus_tag="PWS53_000976"
     CDS             3115..4386
                     /gene="serS"
                     /locus_tag="PWS53_000976"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL0871236.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="PWS53_000977"
     CDS             complement(4935..6419)
                     /gene="guaB"
                     /locus_tag="PWS53_000977"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL0871237.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6924..7619)
                     /locus_tag="PWS53_000978"
     CDS             complement(6924..7619)
                     /locus_tag="PWS53_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL0871238.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="PWS53_000979"
     CDS             complement(7709..8809)
                     /gene="ychF"
                     /locus_tag="PWS53_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL0871239.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8818..9009)
                     /locus_tag="PWS53_000980"
     CDS             complement(8818..9009)
                     /locus_tag="PWS53_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL0871240.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(9030..9920)
                     /locus_tag="PWS53_000981"
     CDS             complement(9030..9920)
                     /locus_tag="PWS53_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL0871241.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9907..10674)
                     /locus_tag="PWS53_000982"
     CDS             complement(9907..10674)
                     /locus_tag="PWS53_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0871242.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="PWS53_000983"
     CDS             complement(10686..11399)
                     /gene="rsmG"
                     /locus_tag="PWS53_000983"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL0871243.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="PWS53_000984"
     CDS             complement(11631..13532)
                     /gene="mnmG"
                     /locus_tag="PWS53_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL0871244.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="PWS53_000985"
     CDS             complement(13549..14946)
                     /gene="mnmE"
                     /locus_tag="PWS53_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL0871245.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15245..15628
                     /locus_tag="PWS53_000986"
     CDS             15245..15628
                     /locus_tag="PWS53_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871246.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15796..16554)
                     /locus_tag="PWS53_000987"
     CDS             complement(15796..16554)
                     /locus_tag="PWS53_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL0871247.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16572..17390)
                     /locus_tag="PWS53_000988"
     CDS             complement(16572..17390)
                     /locus_tag="PWS53_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0871248.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="PWS53_000989"
     CDS             complement(17387..17743)
                     /gene="rnpA"
                     /locus_tag="PWS53_000989"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL0871249.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="PWS53_000990"
     CDS             complement(17861..17995)
                     /gene="rpmH"
                     /locus_tag="PWS53_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL0871250.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18518..19852
                     /gene="dnaA"
                     /locus_tag="PWS53_000991"
     CDS             18518..19852
                     /gene="dnaA"
                     /locus_tag="PWS53_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL0871251.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            20050..21180
                     /gene="dnaN"
                     /locus_tag="PWS53_000992"
     CDS             20050..21180
                     /gene="dnaN"
                     /locus_tag="PWS53_000992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL0871252.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21409..21651
                     /gene="yaaA"
                     /locus_tag="PWS53_000993"
     CDS             21409..21651
                     /gene="yaaA"
                     /locus_tag="PWS53_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL0871253.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21638..22762
                     /gene="recF"
                     /locus_tag="PWS53_000994"
     CDS             21638..22762
                     /gene="recF"
                     /locus_tag="PWS53_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL0871254.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22759..24705
                     /gene="gyrB"
                     /locus_tag="PWS53_000995"
     CDS             22759..24705
                     /gene="gyrB"
                     /locus_tag="PWS53_000995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL0871255.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24725..27196
                     /gene="gyrA"
                     /locus_tag="PWS53_000996"
     CDS             24725..27196
                     /gene="gyrA"
                     /locus_tag="PWS53_000996"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL0871256.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27432..27731
                     /locus_tag="PWS53_000997"
     CDS             27432..27731
                     /locus_tag="PWS53_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL0871257.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27781..28293
                     /gene="ssb"
                     /locus_tag="PWS53_000998"
     CDS             27781..28293
                     /gene="ssb"
                     /locus_tag="PWS53_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0871258.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28319..28555
                     /locus_tag="PWS53_000999"
     CDS             28319..28555
                     /locus_tag="PWS53_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL0871259.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28698..30572
                     /locus_tag="PWS53_001000"
     CDS             28698..30572
                     /locus_tag="PWS53_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL0871260.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDGLIG
                     ISARSTGTINVQIIMESLGGGGHFTNAATQLENTTVEETKQKLFAAIKENMNQIYDKE
                     "
     gene            30578..31030
                     /gene="rplI"
                     /locus_tag="PWS53_001001"
     CDS             30578..31030
                     /gene="rplI"
                     /locus_tag="PWS53_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL0871261.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31304..32671
                     /gene="dnaB"
                     /locus_tag="PWS53_001002"
     CDS             31304..32671
                     /gene="dnaB"
                     /locus_tag="PWS53_001002"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL0871262.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT        10104 a         6530 c         6564 g        10180 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcaggag tgtttcccga
      361 ttcgtttttg gctgccgtag caacttttat atcatatcag agagacaagt aattgtcaac
      421 tcttttttaa aagttttttc gatttcgttt ttcgaaatct tccagcaact catttatcat
      481 aacttatcgt ttgttatttg tcaagaactt ttttgaagtt tttttcgata gaatcaagct
      541 tcgcaagtgg ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa
      601 caacttattt tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat
      661 caacatcaca tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat
      721 gatcattggt tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat
      781 ccttttgttt tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca
      841 ccttctaaaa ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag
      901 aataatagta acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat
      961 gataaagaaa tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta
     1021 aaggcttatg atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta
     1081 cttattttag atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga
     1141 aaagaatccc aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc
     1201 aaaggactgg tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta
     1261 atggctagag taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct
     1321 gatgtattag aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc
     1381 gatgggaaag aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt
     1441 catcctaatc gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt
     1501 attgtctctg ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa
     1561 gcaactggtg gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca
     1621 agatagtaaa cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag
     1681 cgaattgtta gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat
     1741 tctggttgtt atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga
     1801 ttcaaagaat atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt
     1861 tattattccc tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag
     1921 atatcgccaa atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa
     1981 ttatgatcat cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat
     2041 caatggattg gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc
     2101 aaaagatgaa ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat
     2161 tggataccta ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta
     2221 tacgcatact gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga
     2281 atacactaag gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca
     2341 gttgatcgaa cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat
     2401 acttgttcaa tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg
     2461 tgtcttcaat aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt
     2521 aatcgaagta gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat
     2581 gatcccacaa caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg
     2641 ttcacaagca actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt
     2701 gcacggcgga tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct
     2761 tccaatcaag aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg
     2821 aaatattcgc tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca
     2881 ctttattctt tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt
     2941 tccagttcgt ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt
     3001 tcaacaagtg cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc
     3061 caagaataat gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta
     3121 gatgtaaaaa tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc
     3181 gtaaaaaaag aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta
     3241 gtcaaaagtg aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg
     3301 aagcgtgcaa aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac
     3361 atcaaagcat tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact
     3421 actttgccaa accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta
     3481 gaaatccgtc gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa
     3541 attgcagaaa atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt
     3601 tttgtctatt acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt
     3661 gatcagcata tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt
     3721 aaagcgatgt ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag
     3781 aatactgatc taacattaat cccaacagct gaagtaccat taactaacta ttataatgat
     3841 gaaattttag atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt
     3901 tctgaagccg gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat
     3961 aaagtagaaa tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg
     4021 acagctgatg cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta
     4081 tcaactggag acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct
     4141 gcacaagata cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc
     4201 cgcgcaatga tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat
     4261 ggttcaggac ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa
     4321 gatggttctg tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt
     4381 aaataaagag gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa
     4441 tggctttcca catttttgca taaattggac ttaataccga gaaactggct tttgaacacc
     4501 atttatcgag aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa
     4561 aatagtccct catattttac cgtttgttga actaataccg agggtcagct gattgaacac
     4621 cgtttagtgg agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa
     4681 aaacagctct tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac
     4741 tgtttattca aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc
     4801 acgaaaattg cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta
     4861 cttccgccgt ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac
     4921 gctctttaat gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca
     4981 tcatgagggt gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca
     5041 cgtaattgct gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc
     5101 atttggaaaa taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct
     5161 ggtactaatt tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt
     5221 tccatcgcgc ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt
     5281 tcgaattcac ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt
     5341 ccgccggcag ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata
     5401 atcgctttgc catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt
     5461 acacctacac cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt
     5521 acgacatcta cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct
     5581 gcaatcaatg ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct
     5641 gcactatgtc catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca
     5701 ttcgcacgct caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca
     5761 tgttcatctt ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc
     5821 aatccgctta aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc
     5881 aaaatctttt ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt
     5941 gtcatcacat cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta
     6001 attatcccta ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga
     6061 tatttgctca tcaattcttc tgcgtctgct acaagatgct gtggcgttaa gaagaacgga
     6121 tcaatgatta cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg
     6181 atcgtcatgt ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc
     6241 ttgctgtcgg taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt
     6301 ttagctaatt gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc
     6361 aataagacat catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt
     6421 tttagtacca ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt
     6481 caatgatcta cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca
     6541 tctgtgtata ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc
     6601 aatcaaaaac agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact
     6661 tttgaacacc gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa
     6721 aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg
     6781 gcttttgaac accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt
     6841 actattattt aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca
     6901 aaatagctga tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg
     6961 tattttttct tcaagtagta acggattgct aaagcagttg ccccgataat gataactact
     7021 actggatcta atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg
     7081 acccaaagaa gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg
     7141 cttcgatctg ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc
     7201 cagccaccga ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca
     7261 cgtgaaaaca atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta
     7321 ttatctaacc acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg
     7381 attgcttccg ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt
     7441 tgttctgcaa ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac
     7501 aaattcgctg cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt
     7561 aattttgttt ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt
     7621 atctaatagt cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc
     7681 atattctaca gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc
     7741 cgtcttgtac gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat
     7801 gcatgtttcc gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc
     7861 gttcgaaatc tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg
     7921 tccaagcacg tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat
     7981 aagcagcacg gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt
     8041 ccgctttgtc ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga
     8101 tcacttctgc attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt
     8161 ctgcatctgc tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca
     8221 ataaaaatag tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag
     8281 attttccttc ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat
     8341 ctttatcttt ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta
     8401 agtctgctaa cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc
     8461 cttctacgtg agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta
     8521 cttggcggat atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta
     8581 cgattccagc aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa
     8641 gttctgttaa gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga
     8701 tcgtcgcaaa gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag
     8761 ttgattttcc tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca
     8821 ctctttctct gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt
     8881 catcatgacg atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat
     8941 gacttcccag cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa
     9001 gtcgtacata aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata
     9061 tcaagaattc gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca
     9121 tttttttctt ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct
     9181 cggatataat atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca
     9241 ttttgattta actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact
     9301 gcacgattgg ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct
     9361 tgtcccatag ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc
     9421 aagcgcaaat aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct
     9481 tgtgttagtt tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc
     9541 agatcttctc gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag
     9601 tctctaacga tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc
     9661 tcccctgcaa taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag
     9721 acacctgatt gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta
     9781 cggggctgat acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct
     9841 tttacatcca ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct
     9901 aatcctttac ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc
     9961 tcctcgagaa cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa
    10021 acgaatattt cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc
    10081 ttctacgact tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc
    10141 gatctctaat tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct
    10201 taatccttcc aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt
    10261 aaatgaattg attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata
    10321 ctgactgctt acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt
    10381 caattcgatc tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg
    10441 ttcagtgatc aaagttgcct catcaattgg caattcattg actagaacat catagatgtc
    10501 tcttgtcaca tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat
    10561 atccactagc aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt
    10621 cgttgttttg ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc
    10681 tcctcctatt tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg
    10741 gttggtttct ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta
    10801 accgcctctt ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc
    10861 aattcctctt cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag
    10921 ggcaaacaca attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca
    10981 aatgatgcac gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa
    11041 gcatcgattc ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa
    11101 tcaacgatcg ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct
    11161 gcccccgaac caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat
    11221 gtaagtgaat cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg
    11281 ttcatctttt cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga
    11341 tgatcggtaa gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata
    11401 aatattcctt tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca
    11461 aaatctctag acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag
    11521 ccgatagact ttttctttct agtatgactc ataaaatata cacagaagag agaactagct
    11581 ttgagcaatt tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt
    11641 tcactgactt tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca
    11701 gggtttacgc cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat
    11761 ttttgttttg cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc
    11821 tttttcgctt ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct
    11881 tcatatttga tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct
    11941 ggaataaacc gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct
    12001 agtacaccat cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct
    12061 gttggtttga ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc
    12121 ttcaaataat tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt
    12181 aatcgcaaat ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc
    12241 aaacgataag gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa
    12301 ccgtcacttc gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata
    12361 ccggcaatca gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca
    12421 gctgtataaa gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct
    12481 actacgtcat attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca
    12541 atagaatgaa gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca
    12601 tagacttctt ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct
    12661 gcaaaacgca cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg
    12721 atccctgtaa acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca
    12781 cctgtatagg tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg
    12841 aagctaaaat gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc
    12901 gtacttgatt ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt
    12961 tcttttagat gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat
    13021 ttcaattcac cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct
    13081 ttggcccggt agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg
    13141 atcagttctt cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt
    13201 ttcatttctg ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg
    13261 ccttttcctg tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt
    13321 tctccgccta acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa
    13381 ggattacatg gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat
    13441 cccatacgtg cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc
    13501 acgtcatacg catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa
    13561 acaaaattga ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc
    13621 tcctagatag tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga
    13681 atcaatacct gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg
    13741 gcgggtatta gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc
    13801 agaaatagct gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac
    13861 tggttcattt tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt
    13921 tttatttaga agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc
    13981 tgctgtcagt ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc
    14041 ttttcggctt ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc
    14101 tgtatcgatt agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg
    14161 agtcgttcct gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa
    14221 acttgatttg ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac
    14281 cttcccttgc ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt
    14341 ttcaagcaat aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt
    14401 tacttctact tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga
    14461 cagattgccg tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat
    14521 caaatccatc acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt
    14581 tgtgaattcg cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg
    14641 attgactaca acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt
    14701 ctttggtgct ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc
    14761 aacgatatgt ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga
    14821 ttgataaact ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat
    14881 tgcaccttct ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc
    14941 agccattttt tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt
    15001 atgcacaata aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca
    15061 tagattatca cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc
    15121 tgttctgctt ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt
    15181 ttattcgaag agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca
    15241 ctaaatggta gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt
    15301 cagatctgct gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac
    15361 agggaatatc aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga
    15421 agctgatttg tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa
    15481 attaaagacg aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta
    15541 tcagcaaaaa aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat
    15601 ccaatttatc caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg
    15661 aagacagttt cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg
    15721 ataagaatga tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt
    15781 caaaaagtaa tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg
    15841 ttcctcacct tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg
    15901 tttacgttca aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt
    15961 ttcagccgtg cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc
    16021 tacattgact actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag
    16081 atattgcaat gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt
    16141 ttctaaatgg aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc
    16201 atcaagctgt tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc
    16261 acctagctca cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat
    16321 ctcagctgtt ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac
    16381 tttttcgctg atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa
    16441 accttttttt ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc
    16501 agccagacct agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat
    16561 tacctcccct attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct
    16621 ctttcgcgga ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg
    16681 atcagcagcg tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg
    16741 ctggctaaat tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc
    16801 attgatgcat tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag
    16861 attgctgcta aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag
    16921 aaatgccctt gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt
    16981 ggcatttgta ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat
    17041 aaacgctgct gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt
    17101 tgctgcagtg cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg
    17161 aaatgcatca acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc
    17221 cctgcattac caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga
    17281 tcccaaattc ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg
    17341 accaaactaa cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt
    17401 cctctctgtc gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat
    17461 cataagtcaa ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa
    17521 tttcttcttt tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta
    17581 ccgcgttgcc gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt
    17641 tttccaaacg gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg
    17701 cttgaaactc tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt
    17761 ctatctctca cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc
    17821 taaaaaagag tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc
    17881 ttcctttgcg acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac
    17941 ggaatccgtg aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt
    18001 ccacctccaa aatgttctta tctattttat aaacacagac atactttgat agtataacga
    18061 caaagtagtg attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga
    18121 ttattttttt ctatgggaat cctatgtgga aaaagttatg cacagggatt atccacagat
    18181 aataaaaggc tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggc
    18241 gatttcctgt gtattccgtc tcctaactat tggtatatct gtatttttta tccacagacg
    18301 caagaggaaa tctattggtt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac
    18361 tttttttgac aactgtgttt ttctcttttt tctttttgtc gattgtgtgg ataattgtaa
    18421 ttaaaaaatt tttttcttcg gtttctgtgg aaaactattg aaaaaaatgc taaattagta
    18481 ctagttattc actttttaat gaaggaggag gccttatatg gtatccctcg atgctttatg
    18541 gaatgaatta aaagcaacat accaaaagga tttatcgcct gctagctata atacatggat
    18601 cgaaacagcg aaccctcgaa cccttgatca aaaccagctc gttgttgagg ttcctagcaa
    18661 gattcataaa gaatattggg aaaaaaacct ggcgacaaaa atcgttgaag tcggttatat
    18721 gttatcagga aatgagatta tccctcgctt tattactggc gaagaagcag agcaagaaga
    18781 agttatagaa gaaaaaaatc caaaagttgt ggcacctagt ccgctaaaaa aagccatgct
    18841 gaaccctaaa tatacctttg atacgtttgt cattggtaaa ggaaaccaga tggctcatgc
    18901 agcagcgctc gttgtagcag aagatccagg ttctatttat aacccgctct tcttttatgg
    18961 aggcgtggga cttggtaaga ctcacttgat gcatgcaatt ggtcatcaaa tgctgcaaag
    19021 tcagcctaat gccaaagtaa aatatgtaag cagtgaaact tttgctaatg atttcatcaa
    19081 ttcgatccaa aacaaaacag cagaagagtt ccgtcaagaa tatcgaaatg ttgatttact
    19141 gctagtggat gacattcaat tttttgcaga aaaagaagct acgcaagaag agtttttcca
    19201 tactttcaat gccttgtaca atgaagggaa acaaatcgta ctaaccagtg atcggttgcc
    19261 aaacgagatt ccgaaactgc aagaacgcct tgtttctcgc tttgcatggg ggctgtctgt
    19321 tgatattaca ccgccggatc ttgaaacaag aacagcgatt ttacgtaaaa aagcggcggc
    19381 tgaacgtcta gagatcccag atgacacact aagctacatc gcaggacaaa ttgattctaa
    19441 cattcgtgaa ttagaaggtg cgcttgttcg agtgcaagct tttgctgcga tgaatagtga
    19501 agatatttcg actagtctag ctgcagacgc actgaaaacg ttaaaatcag gaaaaggaca
    19561 tcctcaattg tcaatcctgc agatccaaga agaagtggca aagtattatc acatccaatt
    19621 gaaagatctc aaaggaaaga aacgcgtcaa atctattgtt gttcctagac aaatcgctat
    19681 gtatttggca cgtgagctca cagataattc tttaccgaaa atcggagctg aattcggtgg
    19741 caaggatcat acaacggtta tccatgctca tgaaaaaatc cagcagctga tggattctag
    19801 tgtcagtatg caaaatgaag tatcagaaat caaaaacttt ttactaaatt aatttgtcga
    19861 ttctatcaac tgaaaatagc atgtggataa aagaaaaaaa cgttcaaaag ttatacacac
    19921 tttttccaca gctgtttttt cgtttctctc ttacgttttt tgagttttcc acagaatcaa
    19981 catgccctat tacttttatt attcttttta actaataata aataaacaaa cattcaaagg
    20041 agatacatca tgaaagttac tttaaaccga gctagcttta tgcaggaatt gcaaactgtt
    20101 caacgagcta tttcaagcaa aaccacgatc cctattttga caggtgtaaa aatcacactg
    20161 acacaagaag gtttgacttt gacggggagc aacgctgata tatcaattga aacttttttg
    20221 tctgttgaaa acgaaaaagc aaatatgcaa atcgaatcta ctggttccat tgttttacaa
    20281 gcacgtttct ttagcgaaat cattcggaga cttcctgaag aaacatttac tttagaagtt
    20341 ttagaaaata aacaagtagc gatcacttct ggaaaagcga attttatcgt aaatggatta
    20401 gatgcagata actatcctca tcttcctgtt gtcgaaagcc ataaccagat gaaattacct
    20461 gtacacgtat tgactaaact aatcaacgaa acagtttttg ctgtctcaca acatgagagt
    20521 cgtccaatct tgacaggtgt gcatttcatt ttatctgata attctttatt agctgtagct
    20581 actgattctc accgtctaag tcaacgcgtg attccagtag aacaagcggc tgatcatttt
    20641 gatattgtta ttcctggaaa aagtttgatc gaattatctc gctcattaac aaatgaagaa
    20701 gaaatcgtcg aaatcagcat tatggaaaac caagtgttgt tcaaaacaga aacgatgtat
    20761 ttctattccc gtttgctaga aggaaactat ccagatacca atcgtttgat tccttcaagt
    20821 tttaatacag aggttgaatt ttctgttcct agctttttag cggcgatcga acgggcttct
    20881 ttactttctc atgaaggccg taacaacatc gttcgtttat ctattcgtcc agatgcggtt
    20941 gttttatacg gaaattcgcc agaaatcggg aaagtagaag aaagcttgag ttatacagca
    21001 agcagtggcg atccattaga tatttctttc aatccggatt atatgaaagc agcgttgcgt
    21061 gcatttggag atatgagcat caaagtgaaa tttatctctg ctattcgtcc atttacattg
    21121 gaaccaacgg aagatggcgt tcaatttatt cagctgatta ctcctgtacg tacaaattaa
    21181 caatgatttt caatgttctt gaaaatttaa ttgaaaagaa atagaaaaaa taaaatttta
    21241 aacagaaggc cctaaaatct cttttaaggc tttctgttta tttttgttta aatcaccttt
    21301 tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta ttgcgaaaaa
    21361 aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt gaaacaacaa
    21421 gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt cacagttatc
    21481 ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt cgatggcgaa
    21541 ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt tccagaagta
    21601 ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata agctgtattt
    21661 aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc tcgtcatctt
    21721 tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg tattagctat
    21781 gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg acgatcaggc
    21841 gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa tcttattatc
    21901 taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa gtacttacgt
    21961 gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa aaggcagccc
    22021 gcagaccagg cggaagtttt tagatatgga attaggccag atcaatccaa tctatcttta
    22081 tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga agcaattgaa
    22141 tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac tagcagcgtt
    22201 tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt tttgggctaa
    22261 ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt atctcaccgc
    22321 agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc tcgcactttt
    22381 gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc ctcatcgtga
    22441 cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc aaggacagca
    22501 aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag aagagacagg
    22561 ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata atcgtcaact
    22621 ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa cgacacttga
    22681 ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac aagggaaact
    22741 aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc taaagagtat
    22801 gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg acctggcatg
    22861 tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat tgtcgataat
    22921 tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat tgaaaaagac
    22981 aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca ggcaaaaact
    23041 ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa attcggcggt
    23101 ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt taacgcgctt
    23161 tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca agaatatcgt
    23221 cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca tggaacaacg
    23281 gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga tttttcaaaa
    23341 ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat atcgattgaa
    23401 gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg gattaaaagt
    23461 tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt atatttggaa
    23521 ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg ctaccattcc
    23581 aatatcatga gttttgccaa caatatccat acgtatgaag gcggaacaca tgaatcagga
    23641 ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa gctgatgaaa
    23701 gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc tgttatttct
    23761 atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg caattccgaa
    23821 gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt gctggaaaac
    23881 ccaagtgtgg gccgtcaaat tgttgaaaaa ggattactag cttccagagc tcgtctagca
    23941 gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag taatctgcca
    24001 ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt catcgtcgaa
    24061 ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca agccatcttg
    24121 cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat cttggcaaat
    24181 gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga ttttgatgta
    24241 tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga cggcgcgcat
    24301 atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt tgaagctgga
    24361 tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa tattacgtat
    24421 gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc acttcctgcc
    24481 tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga tcatcaacta
    24541 tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt ggacgatgct
    24601 attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc ccgtcgtgca
    24661 ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg aaggaggaaa
    24721 aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca gcgaaatgaa
    24781 agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc ctgatgtcag
    24841 agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat taggtgtaac
    24901 accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg gtaaatacca
    24961 tcctcatgga gactatgcga tctatgagtc aatggtacgg atggcacagc cattcagcta
    25021 ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg acggtgccgc
    25081 cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc tgcgtgatat
    25141 caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag aaccagttgt
    25201 actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg ccgtaggtat
    25261 ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg atttgctgat
    25321 ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac cagattttcc
    25381 aacaggtggt ttggttatgg gaaaatcagg aatccgaaga gcgtatgaaa caggtaaggg
    25441 ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta aagaacggat
    25501 catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac ggatttctga
    25561 actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat cttcccgtga
    25621 aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatctgtta tcttgaataa
    25681 cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg cgatcgaaaa
    25741 aggtgtgccg aaagttttga gcttgaaaca aattcttgaa aactatatcg aacatcagcg
    25801 cgaagtcatc acaagacgga cagtctttga aaaagaaaaa gcagaagcaa gggcacacat
    25861 tttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga ttatccgcgg
    25921 gtctaaatca gatgacgaag caaaagcgac gatgatcgaa cggttcgatt tatccgatcg
    25981 ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg aacgggaaaa
    26041 aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg acattctggc
    26101 aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga atcaacgatt
    26161 tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg aagatgaaga
    26221 tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca tcaaacgttt
    26281 ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag gaatgggtat
    26341 ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata cattgttatt
    26401 ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag aatacgggcg
    26461 cacagccaaa ggaataccaa ttatcaattt actaggtatc gattcgagtg aaagcatcca
    26521 ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct ttacgacgcg
    26581 tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa gtaatggtct
    26641 gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga cagatggtca
    26701 atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag aagaagcagt
    26761 ccgcgatatg ggacgtacag cttccggggt tagaggaata cgcctgcgtg aggaagatta
    26821 tgtcgttggg gcagccttaa tgaaggaaga tcaagaagta ttagtgatca cagaaaaagg
    26881 ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag gtaaagggat
    26941 caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca ctgtttctgg
    27001 agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca acgtatcgac
    27061 tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg aagctgatac
    27121 gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag ttgaagaaca
    27181 aggatcagaa gaatagccat caagactaat tgtgtaagct agcaagaatg accgattaaa
    27241 aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa atattcgaaa
    27301 ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aatcagagct tgtgagtagt
    27361 ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag tccacaggga
    27421 ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt ccaaacattg
    27481 atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa gataacggag
    27541 cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa atgaacggtt
    27601 accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca aatgctgtca
    27661 atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg attgttaaag
    27721 aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga gatgactatt
    27781 ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt gcgttacaca
    27841 gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt tacaaaccaa
    27901 agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc agcagaaact
    27961 ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat ccaaactcgt
    28021 tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc tgacaacttc
    28081 caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg attccaaagt
    28141 tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac atctcaatca
    28201 tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc aagttcaacg
    28261 atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg gaaatagaat
    28321 ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg ctgctaacca
    28381 tattgaatat atcgattata aagatatcga attactaaaa agatttattt cagaacgcgg
    28441 taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta aaattactgt
    28501 tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag actaataaat
    28561 cctaggaatt gaatgactga tccttttgat cagctattca attccttttt ttctgtaaaa
    28621 ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc aaattagtca
    28681 tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa cctctcttcc
    28741 ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt cgttttgtta
    28801 gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg aaaatgaaac
    28861 gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta gctgaaacca
    28921 atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag ccatcaagcc
    28981 aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt ggattaacag
    29041 aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac caagtctatc
    29101 tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt tattttaatg
    29161 atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg gtgattggta
    29221 ttttatctat tgataactat gcagatgcca ttgataaatt ggatgacaaa gagatttctt
    29281 atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac catgtctttt
    29341 tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat ctgaaaaaga
    29401 tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa aaacagagct
    29461 cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat gaaatcggtg
    29521 gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa gttgtcgtga
    29581 aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct aagaatgtca
    29641 aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt tttgatgaaa
    29701 caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta ggctctgctt
    29761 ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt atcaacgaga
    29821 acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat cctgaattag
    29881 aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag gacctcctcg
    29941 tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat gagcaatttg
    30001 ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt aaacctctgc
    30061 tgacttatat cgaatcttca gcttcttcag cttcagagct agtttctgag ctgatccaat
    30121 accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg cttgcaggga
    30181 tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt gacgttgcca
    30241 gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg agctcggatt
    30301 tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg acttctgata
    30361 ttgttgtggc ggcagggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30421 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30481 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30541 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30601 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30661 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30721 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30781 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30841 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30901 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30961 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    31021 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    31081 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    31141 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    31201 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31261 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31321 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31381 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31441 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31501 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31561 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31621 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31681 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31741 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31801 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31861 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31921 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31981 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    32041 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    32101 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    32161 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    32221 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32281 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32341 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32401 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32461 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32521 agatgacgat gatggaaacg agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa
    32581 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32641 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32701 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32761 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32821 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32881 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32941 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    33001 cagttttttg aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctttc
    33061 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    33121 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    33181 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33241 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33301 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33361 aaaaataaaa atcattcg
//
LOCUS       DANJJH010000023        32178 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000023 DANJJH010000000
VERSION     DANJJH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(407..766)
                     /locus_tag="PWS53_001003"
     CDS             complement(407..766)
                     /locus_tag="PWS53_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL0871263.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="PWS53_001004"
     CDS             complement(796..1137)
                     /locus_tag="PWS53_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871264.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="PWS53_001005"
     CDS             complement(1134..1808)
                     /locus_tag="PWS53_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0871265.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWS53_001006"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="PWS53_001006"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL0871266.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="PWS53_001007"
     CDS             complement(4322..5512)
                     /locus_tag="PWS53_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0871267.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="PWS53_001008"
     CDS             complement(5533..6060)
                     /locus_tag="PWS53_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL0871268.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="PWS53_001009"
     CDS             complement(6107..8896)
                     /locus_tag="PWS53_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0871269.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="PWS53_001010"
     CDS             complement(9043..9243)
                     /locus_tag="PWS53_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0871270.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="PWS53_001011"
     CDS             complement(9696..10016)
                     /locus_tag="PWS53_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871271.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="PWS53_001012"
     CDS             complement(10519..11637)
                     /locus_tag="PWS53_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871272.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="PWS53_001013"
     CDS             12108..13415
                     /locus_tag="PWS53_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL0871273.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="PWS53_001014"
     CDS             13536..14735
                     /locus_tag="PWS53_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0871274.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="PWS53_001015"
     CDS             complement(14762..16030)
                     /locus_tag="PWS53_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL0871275.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="PWS53_001016"
     CDS             complement(16200..16841)
                     /locus_tag="PWS53_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL0871276.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="PWS53_001017"
     CDS             complement(17250..17753)
                     /locus_tag="PWS53_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0871277.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="PWS53_001018"
     CDS             complement(17876..18640)
                     /locus_tag="PWS53_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL0871278.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="PWS53_001019"
     CDS             18748..19023
                     /locus_tag="PWS53_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL0871279.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="PWS53_001020"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="PWS53_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0871280.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="PWS53_001021"
     CDS             complement(20579..20980)
                     /locus_tag="PWS53_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0871281.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="PWS53_001022"
     CDS             complement(20994..21803)
                     /locus_tag="PWS53_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0871282.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="PWS53_001023"
     CDS             complement(21790..22680)
                     /locus_tag="PWS53_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0871283.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="PWS53_001024"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="PWS53_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="PWS53_001025"
     CDS             complement(23348..24289)
                     /locus_tag="PWS53_001025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL0871284.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWS53_001026"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="PWS53_001026"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0871285.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="PWS53_001027"
     CDS             complement(25274..26470)
                     /locus_tag="PWS53_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0871286.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="PWS53_001028"
     CDS             complement(26488..27216)
                     /locus_tag="PWS53_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0871287.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="PWS53_001029"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="PWS53_001029"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0871288.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="PWS53_001030"
     CDS             complement(28628..29086)
                     /locus_tag="PWS53_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871289.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="PWS53_001031"
     CDS             complement(29083..30546)
                     /locus_tag="PWS53_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0871290.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="PWS53_001032"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="PWS53_001032"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0871291.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="PWS53_001033"
     CDS             31602..>32178
                     /locus_tag="PWS53_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0871292.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9386 a         6921 c         5510 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtagac
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DANJJH010000024        32176 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000024 DANJJH010000000
VERSION     DANJJH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(114..494)
                     /gene="yajC"
                     /locus_tag="PWS53_001034"
     CDS             complement(114..494)
                     /gene="yajC"
                     /locus_tag="PWS53_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL0871293.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(591..1736)
                     /gene="tgt"
                     /locus_tag="PWS53_001035"
     CDS             complement(591..1736)
                     /gene="tgt"
                     /locus_tag="PWS53_001035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL0871294.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1954..2652
                     /locus_tag="PWS53_001036"
     CDS             1954..2652
                     /locus_tag="PWS53_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0871295.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2820..5459)
                     /locus_tag="PWS53_001037"
     CDS             complement(2820..5459)
                     /locus_tag="PWS53_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0871296.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6078..7109)
                     /gene="queA"
                     /locus_tag="PWS53_001038"
     CDS             complement(6078..7109)
                     /gene="queA"
                     /locus_tag="PWS53_001038"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL0871297.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7302..8477
                     /locus_tag="PWS53_001039"
     CDS             7302..8477
                     /locus_tag="PWS53_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0871298.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8481..9122
                     /locus_tag="PWS53_001040"
     CDS             8481..9122
                     /locus_tag="PWS53_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871299.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9119..10036
                     /locus_tag="PWS53_001041"
     CDS             9119..10036
                     /locus_tag="PWS53_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871300.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10033..10704
                     /locus_tag="PWS53_001042"
     CDS             10033..10704
                     /locus_tag="PWS53_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871301.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11245..11733)
                     /locus_tag="PWS53_001043"
     CDS             complement(11245..11733)
                     /locus_tag="PWS53_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL0871302.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11934..12200)
                     /locus_tag="PWS53_001044"
     CDS             complement(11934..12200)
                     /locus_tag="PWS53_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0871303.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12200..12463)
                     /locus_tag="PWS53_001045"
     CDS             complement(12200..12463)
                     /locus_tag="PWS53_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0871304.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12939..13124
                     /locus_tag="PWS53_001046"
     CDS             12939..13124
                     /locus_tag="PWS53_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0871305.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13266..15089)
                     /locus_tag="PWS53_001047"
     CDS             complement(13266..15089)
                     /locus_tag="PWS53_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0871306.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15687..18392)
                     /locus_tag="PWS53_001048"
     CDS             complement(15687..18392)
                     /locus_tag="PWS53_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL0871307.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18698..19645)
                     /locus_tag="PWS53_001049"
     CDS             complement(18698..19645)
                     /locus_tag="PWS53_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL0871308.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19835..20104
                     /gene="rpsN"
                     /locus_tag="PWS53_001050"
     CDS             19835..20104
                     /gene="rpsN"
                     /locus_tag="PWS53_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0871309.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20280..20642)
                     /locus_tag="PWS53_001051"
     CDS             complement(20280..20642)
                     /locus_tag="PWS53_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0871310.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20653..21774)
                     /locus_tag="PWS53_001052"
     CDS             complement(20653..21774)
                     /locus_tag="PWS53_001052"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL0871311.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21790..22140)
                     /locus_tag="PWS53_001053"
     CDS             complement(21790..22140)
                     /locus_tag="PWS53_001053"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL0871312.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22270..23781)
                     /locus_tag="PWS53_001054"
     CDS             complement(22270..23781)
                     /locus_tag="PWS53_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0871313.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24103..25455)
                     /locus_tag="PWS53_001055"
     CDS             complement(24103..25455)
                     /locus_tag="PWS53_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL0871314.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25608..26684)
                     /locus_tag="PWS53_001056"
     CDS             complement(25608..26684)
                     /locus_tag="PWS53_001056"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL0871315.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26880..28625
                     /locus_tag="PWS53_001057"
     CDS             26880..28625
                     /locus_tag="PWS53_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL0871316.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28905..29342)
                     /locus_tag="PWS53_001058"
     CDS             complement(28905..29342)
                     /locus_tag="PWS53_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL0871317.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29326..30699)
                     /locus_tag="PWS53_001059"
     CDS             complement(29326..30699)
                     /locus_tag="PWS53_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871318.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31021..31509)
                     /locus_tag="PWS53_001060"
     CDS             complement(31021..31509)
                     /locus_tag="PWS53_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871319.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31531..32133)
                     /locus_tag="PWS53_001061"
     CDS             complement(31531..32133)
                     /locus_tag="PWS53_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0871320.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9197 a         6879 c         5941 g        10159 t
ORIGIN      
        1 cgattattca atattgagca atatatccca tgctcttatc tctttctttt ttgaataaaa
       61 aatgaggtag gacatctttc tgccctacct cgtctttaaa taagctttga tacttatttt
      121 tcttccttct cttctgtctc ttccagcgtt tctgttgttt cagtttttac ttcaactgtc
      181 tcagctggtt cttctgttgt ttcaacagta gtcgttgcat catttgttac gacagtccct
      241 ggtttgaccg ttttgattgc tgcacgatcg tactcaagga agatcccttc gcagtctaaa
      301 acaactgtcc gtttgtcatt gtcgatttct gaaacaactc catgtaatcc gccgatcgtt
      361 acgactgaat cacctggttt catgctatct aacaagtttt gacgttcttg ctgttgtttc
      421 ttttgcgaac gtgacataaa aaaccacatt gctaatagcg cgataaacat cacaagcatt
      481 ggtaatgttc ccattttttt cacctcacta attctgtatc tacaaaaaac actttagcag
      541 aaaaatgctg ataacactag ttttttactt aatttttaaa agattttcct ttagaagctt
      601 tttgcgtttt ctttattgaa cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa
      661 ttatcatcca tgattgcttg acgtacttgt ttcattagat tcaacaagaa gtataaattg
      721 tggtaagaag tcaaacgaat cccaaatgtt tcatcacatt tgatcaaatg gcggatatag
      781 gcacgtgtat agtttttaca tgtataacaa tcacatttct catcaattgg acggaaatca
      841 tgggcatatt tcgcattttt cactactaga cggccttgag aagtcataca tgtgccattt
      901 cgagcgattc gagtaggcaa gacacagtca aacatgtcta ctccacggat aacaccatcg
      961 atcaatgaat cagcagcacc gacacccatc aagtaacgtg gtttgttttc ggggatcaac
     1021 ggtgtagtga attctaatac acgattcatt tctgatttag gttccccgac agataacccg
     1081 ccgattgagt aaccagggaa atccatcccg actagatctt tcgcgctttg acgacgaaga
     1141 tcttcaaacc ctgctccttg gatgattcca aataatcctt gacggtctgg attagcatga
     1201 gctttcaatc ctcgttctgc ccatcgagac gtacgttcta ctgatttttt cacgtagtcg
     1261 taactttcat cgaatggtgg acattcgtca aaactcatca tgatatcaga gcccaattta
     1321 ttttggatgt tgattgcttt ttctggtgat aaaaacatac gcgaaccgtt caagtgattt
     1381 ttgaaatgca cgccttcttc ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag
     1441 ccgcctgaat ctgttaaaat cggttgatcc cagttcataa atttatgcag tccgccagct
     1501 tcttctacta agtcttcacc agggcgtagc cataaatgat atgtattact taaaataacg
     1561 ccagcaccca tttctttcaa ctcttctggg gacattgttt ttactgtagc taatgttcct
     1621 actggcatga acattggtgt ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc
     1681 gcgccagtat gtttttcttt cttgattaaa cgatagcgaa tggcaggttg cgtcattcat
     1741 ttccctactt tctatataaa atatcttgac aactcccacc atcataatcg tttcaagtgc
     1801 tttttgcaag ttccctcggc ttccttctac tttatttaat attctttttt ttatcctttt
     1861 tttatgaata acaaaaaaaa gaaaaacatt atgacagaaa tattttttga tacatggtat
     1921 attggtgtta ttaaagaaaa ggagggaaaa atcatgcaat cttctgcaga attaaaacgc
     1981 caagcaaaag agagtctaaa aggacgttgg ggacaagctg ttttactaaa tctaatccct
     2041 acattgattg gtattgctac tattcttctt attatgattc ctattatctt gtttgccacc
     2101 accatatatg atggatcagc gagcgtctca gaagcgacaa cttcaggagg cggtagttca
     2161 ggaggcggtg gcggaattat ttccagcatc attagcgctt tgttcatgag cggtatttct
     2221 tggacttatc ttgatatttt acgtggacaa aaagatcaga tcgaaccatt caaagatgca
     2281 tttcgtggat tcaaaggttt gtttatcggc ggagtgatca gcttagctgt gctgatctcg
     2341 atctttacaa cattatgggg tttgctatta gttattcctg gaatcattaa aagctattct
     2401 tattctcaaa gctattttat ttactacgat atcgtccaag aaacaggaca aacacctaaa
     2461 gtactggata cgatcactgc aagtcgaaaa ttgatggatg gttataaagg acaacttttc
     2521 tggttagatc tttcatttat cggctggcat atcttagcat tagcaactgc tggtattggt
     2581 tatctatggc tcagcccata catttctgca acaaaagcag cattttatga acaattgcca
     2641 aaagatattt aaaagagagt gtgacttgtg accgaggggg tggcaggaga acaccgttta
     2701 tcgagaggca gtaaaagaaa cattcagccc attagcaaaa aggaggtgtg acaacattca
     2761 tgtcacacct cctttttgta tagttccagt aaaaagaaaa tcaacttaga aacagttaat
     2821 cattcctctt cttctgcttc cggtaatcga tggaacttga tcagttcatt caccgcgatt
     2881 ggtacgatag ataaaatccc taccaagaac cagtgattca aactgatgtg tactaggccg
     2941 aatagttctt gcgtaaatgg cagcaaagca acagccgttg taatagctaa tgccagtaac
     3001 gccatttcta ccaaagcttt attggtcgct aaattgactt tgaagatcga ttggctgctg
     3061 cgtacattga acacatgcag aatcgatgaa taagctaaga tcaagaatgc aacagtttga
     3121 ccgacatgct gacttgcgtc tacccatggg ctgatagtat cgacataact acctaaataa
     3181 taaccaaaca aggttacgat ggtgaagacc cctgcattta tggcaatttt ttgcaataaa
     3241 cctcgagcaa agatcccttc atttttcgga atgggtggct gatccatgat gccgtgttcc
     3301 gctggctctc ttcctaaagc aaatcctgga agtccatcag caactacatt gacaaacagt
     3361 agttgcacag ctgtaaatgg tgctccccat cctaacagca tcgcaatcaa aacaataaag
     3421 atctccgaaa tattacagct tagcaaaaag ttgatagctt tacgaatatt ctgataaacc
     3481 gaacgtcctt gactgacagc gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata
     3541 tctgcggcac tttgcgccac atctgttcct gtgatcccca tggcacaccc aacgttactt
     3601 gctttcaacg ctggtgcatc attcacacca tccccagtca tcgctacgac tgcacccgta
     3661 cgttgccaag atttgacgat ccggatcttg tcttctggcg ttacccgagc atagacagag
     3721 agagctttga cacgcgcatc taattcttcg tctgataatt gatgcagttc tgatcctgaa
     3781 agtgcttctg acggatcatt caagatacct aattctttcg ctatggcact ggcagtcacc
     3841 acatgatctc cagtgatcat gactgttttg atgccagcct ttttcgctcg cttgattgct
     3901 cctttacttt ctggacgtgg tggatcgatc attccgatca gaccaatcaa acgtaagtct
     3961 ttttccagtg cttcagaaga aatatcttgc ggttcttcgt cataagtagc ataaccaaca
     4021 gcaatcactc tcagtgcccg tttaccaaag cgatcattaa cgatcgccgc ttgttctaca
     4081 tcgcctgaac taaagcgcgg agcaagaaca tcgaatgctc ctttggtgat tgagatgtat
     4141 ttttcgccca tttggtgaac tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg
     4201 tttactcgtg gatatttttc ttctaattct gcctttgtat gataattctc ttcaacagcc
     4261 cttacgatgg ctacttctgt tggattacct ttgataacaa ggtcgtcatc ttcttgctcg
     4321 accgtcacat ctgtacatag tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt
     4381 gaatcttcga tattcgttac ttcatcttct ttgctccata cccggcggac gcgcatttca
     4441 ttttgtgtca gcgttcctgt tttgtctgag cagataacat tagcggttcc tagcgtttca
     4501 actgctggca actgtcggat gatcgcatgt ttcctcgcca tcttctgcac accgtatgca
     4561 agtgtcaacg taacaataac agtcaaggtt tccggtacag ctgcaacagc caaggaaatc
     4621 gaagtcatga acatctcaag gagtggttct ccttgcagtt caccaatcac aaatactaaa
     4681 gccgcagccg ctaaagcgat catactgatt cgtttgccta gctgattcaa tcggacctgt
     4741 aaaggtgttt tttgcatagt attttcgttc aaaagatccg cgatcttacc catttctgta
     4801 gccatgccaa tggcagtgac gatcgctttc ccacgacctg cagcaaccgt acatccttta
     4861 aataccatat taagttggtc acctaatgac tcatcttctt cagcaataaa tgctgcatct
     4921 ttttcaactg cttcactttc tccagtcaac gccgattctt ctacacgtaa ttgtgatgct
     4981 tcgatcaaac gggcatcggc agaaatagca tcaccggact ctaaaacaag aatatcaccc
     5041 actaccagtt gctctgactc aacagtggtc actttgccgt ctcgtatgac agttgccgtt
     5101 tgtttattca tatcttgcaa ggattccagt gctttttctg cgtttccttc ttggacaatc
     5161 gccaataccg agttgatcac aacgatagcg atgatcagca gtccttcaaa taaatcccca
     5221 tgttctgtag caaatgcggt ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt
     5281 gtaaaatcgg acaagcttcg aaagaatttt ttgatcatcg attctttcgg cgcctcatcg
     5341 aatttattca atccctgctt ttttcgccgt tcatccactt cttgtgaaga caacccagta
     5401 tctggatctg cttctgttac cttctttact tcttctattg tctgcttgta ttcttccatc
     5461 ctattcctcc tttaaaatct tcgtacaaaa aaaggtactt attttactat aacatccttt
     5521 ttttatttac ttcaaataaa gggcttatcg attatttgtt aagaaagcgt aaaatttaaa
     5581 gtgcttatat ttgcaaacgc taccaaagga tgctattata tagatgtaag gaaggaggat
     5641 accatcaaat agacagaaga atttctcgat agaacagtgt aagcaaacaa agttgttaga
     5701 agtacaggtt tcacccagac atgcgtgtag agtacaaacc tataaatagt gaatgcgtat
     5761 ggatgtttgg atcacgaagt atccgaaaca agaagtaatg cgatacgtaa caattgaata
     5821 acgtaaacgt ctgaaaattg aataggaaca agagatgttt caaatgttga agatgtaacg
     5881 aaaacaaaat ttttgtttaa catgaagaaa ttcaacaccg tgctttgcat gtatgatgga
     5941 aagttacaat catttctgtt tatttgaatc cttccgaggt acaagaaagc cgagcttatc
     6001 gagagctcgg ctttttcttt gtcataaaat ctttgtaaag cttttttgtt attcaaaact
     6061 agcggctttt cataacctta tttcacgaac atcgcatctc caaaactaaa gaaacgatag
     6121 cgctcttcaa tcgcatgctg gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta
     6181 actaacatga ctaaagtcga ttttggcaaa tggaaattag ttgagaaagc ttctaccact
     6241 ttgaattcat agccaggcgt aatgaaaata tcagtccatc cgctgtctgc tttgatttct
     6301 ccattaaatt tcgttccaat cgtttctaat gttcgaattg aagttgttcc gacagccacg
     6361 attttcccgc catttttacg tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg
     6421 tagaattcac tatgcatatg gtgttcttca attttatcta cgctgacagg tcggaatgta
     6481 cctaacccta catgtagtgt cagatagact aattcaacac cctttgcttc gatctcatca
     6541 agtaattctt tcgtaaaatg caatccagct gttggcgctg cagcagaacc attttctttc
     6601 gcatagacag tttgatagcg atctgggtct tccagacgtt ctttgatata tggaggcaat
     6661 ggcatttctc ccaatgattc aagaatctct agaaaaattc cttcatactc aaagtcaatg
     6721 atccgtccgc cgtgttccaa ttcttctttc actactgctt gcaatctgcc atcaccaaaa
     6781 gagattcttg tcccaacttt ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct
     6841 ccttgtgtat tcgttagaag aaggacttct aaatgcgcac cagtatctgg tttttctcca
     6901 tataaacgag caggcagaac tcgtgtatta ttcataacta gcgcatcacc aggctgtaac
     6961 tcttcaatga tttcatggaa atgtttatct tccatctctc cagttttccg atccaaaaca
     7021 agtaaacgag aatgatcgcg gtcttttaat ggtgtttgtg cgattaattc ttccggcaag
     7081 tcaaaatcaa aatcttctgt tgttaacata tcgtcactca ctttctatca tgtgcttcat
     7141 cattctatca tgtttttcgt tctgttggca aaatcagcac tttttcactt ctattttttg
     7201 ttttcctaaa taaaaaaact accctatttg tattttttca ttgttttagg catactagta
     7261 atagatagtt cttcattgaa aatagaaaaa gaggtggcac catgattgaa tttcaaaatg
     7321 tgtcaaaaat ttataaaggt ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa
     7381 agggtgagtt catttgtttt atcggtacga gcggaagcgg aaaaacgacg acgatgcgga
     7441 tgatcaaccg aatgactgat ccaacacaag ggaaaatttt gattgatgga aaatccatcc
     7501 aagaaattga tccagtggaa ttgcgacgtc agatcggcta tgttatccaa agtattggat
     7561 tgatgcctca catgacgatc cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca
     7621 gtcaagaaga acggaataaa acagctgaaa aaatgattga tcttgtggaa ttgccccgtg
     7681 agatgttaga tcgctaccca catgaattat ctggtggtca gcagcagcgt attggagtcg
     7741 ttcgtgccct tgcagcgaat caagatatca tcttgatgga cgagccattc ggtgcgcttg
     7801 atccaatcac tcgtgattcc ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa
     7861 aaacgatcgt atttgtaacc catgacatgg atgaagcgat taaactagca agccgtatcg
     7921 ccatcatgag tgaaggccgt gtgatccaat ttgatacacc agaaaatatt ttacgtcatc
     7981 ctgcaaacga tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa
     8041 atgcgacaag agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat
     8101 cattacaaga agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg
     8161 ataattcgaa tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa
     8221 aagtatccag tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct
     8281 atcttcgtga taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg
     8341 atgaacagaa caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg
     8401 atgtgatttg gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag
     8461 aatctaagga ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct
     8521 caaaaggttt tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg
     8581 ctgtaccgct tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct
     8641 taaccagtgc cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat
     8701 ttggcgtagg taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt
     8761 tacgtaatac gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag
     8821 gcatggggat gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa
     8881 cgatcatggc tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct
     8941 cttatatcgg cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac
     9001 cagatctcat tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc
     9061 ttctaggtct cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat
     9121 gaaacgtata ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc
     9181 cttccctggt cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac
     9241 ttcagaagca cagatcttag caagtctagt tgccggaatg gttgaacatt atacagataa
     9301 aaataccgca atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa
     9361 cggagatgct tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa
     9421 ccttcctcct gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa
     9481 aagatacggt caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt
     9541 tcgaaaagac accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc
     9601 ggatgaatta gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga
     9661 cggcttccaa gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact
     9721 tgtttatgac gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg
     9781 acggatcgcc agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata
     9841 tgatgctgct ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc
     9901 cttaaataag ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc
     9961 agataataat ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta
    10021 ttttgaaggg aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat
    10081 ttccaggaaa atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc
    10141 tatggtgtcc tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga
    10201 cgtaaattag caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta
    10261 gccatgatct ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact
    10321 gtatttttgt attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta
    10381 gacaaaaaca tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc
    10441 atggtagagt taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt
    10501 gttgccatcg ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc
    10561 attatccgag gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca
    10621 actgcattaa tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat
    10681 ccagccagcc gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt
    10741 gttggtcgca aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct
    10801 tccctcctta tgggtaaaga gttgcttttc ttagctattt atccattccg tatactccaa
    10861 cttcatactg ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct
    10921 aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc
    10981 taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc
    11041 tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa
    11101 aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat
    11161 cctcatcctt ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg
    11221 gttcctaaaa ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat
    11281 acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata
    11341 agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct
    11401 attcatggcc gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg
    11461 caatgtcgct ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc
    11521 tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg
    11581 ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc
    11641 aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa
    11701 cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac
    11761 ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac
    11821 aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct
    11881 cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca
    11941 taatgtgtcc aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc
    12001 actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt
    12061 tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat
    12121 tgtttcttta aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt
    12181 gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc
    12241 tactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa
    12301 gggtttcttg aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac
    12361 gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa
    12421 aatttttacg agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt
    12481 ctgatataat tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa
    12541 ttcagcaaac ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt
    12601 tgtccacgct cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt
    12661 aacaacgtat catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac
    12721 caatacacga aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga
    12781 tacgcacgaa agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact
    12841 gcctcaaaaa tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac
    12901 ttgcttagac cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca
    12961 aaaaatggtc acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag
    13021 tcgaggtaaa ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg
    13081 gaaattatga tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa
    13141 catttcatac gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa
    13201 gacaaaacag accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac
    13261 ttggtttatt tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac
    13321 aatcgcaatc ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt
    13381 ggtacaaatt gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt
    13441 acgatatcta cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt
    13501 gaaaaacgga catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg
    13561 acatcttcgt ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa
    13621 ttcaacgcac cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg
    13681 tcataatcat gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa
    13741 tgatcatgga tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa
    13801 atgtccccta accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc
    13861 gtaatattgg caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc
    13921 atccctgttt gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag
    13981 cgttctgttg agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata
    14041 atcccattgg aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta
    14101 tcttttgcca gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact
    14161 gctaaaatca gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca
    14221 gctttaccaa tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag
    14281 ttgataaatg tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta
    14341 gcagcggcat tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc
    14401 accccagtta aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca
    14461 cccggcacag ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata
    14521 ttatataaac caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat
    14581 aataggaacg atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg
    14641 attgtcacag aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct
    14701 tcagcacctg ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca
    14761 ccggaaatca agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg
    14821 ccatgaggat aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata
    14881 ataacaaaag cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa
    14941 acgacaacga tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat
    15001 gccaaagcag caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta
    15061 cctactaaca acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt
    15121 caagttttat agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta
    15181 aaagtcaata aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt
    15241 caaacgcttt aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta
    15301 taataaaagg atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact
    15361 tttggaagag agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg
    15421 cagttgtcac aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc
    15481 aaccaaatta aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga
    15541 gcagcttttt tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct
    15601 caaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca
    15661 aaacactgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct
    15721 ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag
    15781 acaacaaaca aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct
    15841 cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt
    15901 tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt
    15961 actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag
    16021 acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt
    16081 tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc
    16141 gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt
    16201 cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg
    16261 acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga
    16321 taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg
    16381 tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc
    16441 gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc
    16501 gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta
    16561 ccggtgattt cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct
    16621 aatttttgct gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca
    16681 ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca
    16741 attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct
    16801 ttcgttgagc tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt
    16861 gcagtcccaa tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga
    16921 cctgtattga taccagcaat gatgccattg atattattaa caacggcatc aatttgatct
    16981 agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg
    17041 cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt
    17101 aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt
    17161 gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc
    17221 ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa
    17281 cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg
    17341 cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt
    17401 gcctgatttt ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc
    17461 aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg
    17521 ccgtcgatct ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa
    17581 tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact
    17641 ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc tttattcagt
    17701 ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg
    17761 atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt gatacttact
    17821 aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct
    17881 gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt
    17941 ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata
    18001 tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg
    18061 taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat
    18121 cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta
    18181 ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt
    18241 ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg
    18301 atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa
    18361 tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct
    18421 gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat
    18481 ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa
    18541 ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt
    18601 ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa
    18661 atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga
    18721 ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg
    18781 aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta
    18841 tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc
    18901 cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc
    18961 ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta
    19021 caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg
    19081 ctgaagctcc taattttttt accttttcct catttttact ggatgcactg gttaagattc
    19141 ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta
    19201 gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg
    19261 taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg
    19321 ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag
    19381 ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc acaataccag
    19441 cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta
    19501 aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc
    19561 cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt
    19621 aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt
    19681 atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat
    19741 agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt
    19801 tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa
    19861 agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc
    19921 gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa
    19981 aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg
    20041 tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg
    20101 gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat
    20161 caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt
    20221 tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt
    20281 tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact
    20341 tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc
    20401 gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg
    20461 atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg
    20521 atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg
    20581 ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc
    20641 ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac
    20701 ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc
    20761 atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat
    20821 cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga
    20881 aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca
    20941 ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt
    21001 tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg
    21061 tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc
    21121 gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag
    21181 ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc
    21241 atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact
    21301 ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc
    21361 catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa
    21421 aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc
    21481 aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa
    21541 tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc
    21601 acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac
    21661 tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt
    21721 tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt
    21781 tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat
    21841 actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt
    21901 ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga
    21961 gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa
    22021 ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc
    22081 ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat
    22141 atgcttttct cttttcaata aatttagaac actctcattc taccaaatta taaccatttt
    22201 gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc
    22261 attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg
    22321 tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc
    22381 acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt
    22441 attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc
    22501 atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata
    22561 ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt
    22621 tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg
    22681 tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat
    22741 ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc
    22801 gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac
    22861 acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc
    22921 gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc
    22981 ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt
    23041 acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc
    23101 gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc
    23161 tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat
    23221 agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat
    23281 gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac
    23341 taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc
    23401 tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc
    23461 tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc
    23521 ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg
    23581 acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc
    23641 tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg
    23701 gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa
    23761 ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct
    23821 tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca
    23881 cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt
    23941 taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct
    24001 tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt
    24061 tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag
    24121 ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt
    24181 tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa
    24241 cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc
    24301 ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat
    24361 atttaaagac tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt
    24421 tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta
    24481 gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt
    24541 gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc
    24601 gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa
    24661 tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg
    24721 aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg
    24781 tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt
    24841 gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc
    24901 accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc
    24961 attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa
    25021 aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta
    25081 tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt
    25141 aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc
    25201 tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa
    25261 gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc
    25321 tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc
    25381 gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg
    25441 tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa
    25501 acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta
    25561 gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt
    25621 tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc
    25681 aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta
    25741 aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa
    25801 tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc
    25861 gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg
    25921 atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc
    25981 gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt
    26041 tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct
    26101 cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc
    26161 ggataaagca aagaaccttc acattgatca aatacttttt taggattttc tttccattga
    26221 ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg
    26281 gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata
    26341 ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact
    26401 ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag
    26461 ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt
    26521 ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga
    26581 acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact
    26641 tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt
    26701 attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata
    26761 acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca
    26821 gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga
    26881 tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca
    26941 atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag
    27001 aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt
    27061 cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt
    27121 caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg
    27181 atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga
    27241 agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac
    27301 ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg
    27361 tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact
    27421 tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga
    27481 tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa
    27541 tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca
    27601 ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta
    27661 tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat
    27721 tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg
    27781 aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc
    27841 catataccat caactacatc gattttggta tgatggggca tctttctgca ggacttaggc
    27901 aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag
    27961 cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac
    28021 tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag
    28081 tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca
    28141 cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag
    28201 tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg
    28261 tagaagatac attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta
    28321 agatccctca acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca
    28381 atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc
    28441 ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag
    28501 cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg
    28561 cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa
    28621 agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata
    28681 aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa
    28741 caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa
    28801 gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa
    28861 ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt
    28921 tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc
    28981 cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata
    29041 ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca
    29101 agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg
    29161 attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg
    29221 tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat
    29281 tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc
    29341 attctttttc gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc
    29401 aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg
    29461 gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa
    29521 aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac
    29581 aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca
    29641 gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg
    29701 aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca
    29761 tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata
    29821 aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact
    29881 gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca
    29941 gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag
    30001 cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt
    30061 tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac
    30121 cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg
    30181 gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc
    30241 aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt
    30301 cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg
    30361 tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata
    30421 aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag
    30481 caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg
    30541 aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc
    30601 caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg
    30661 tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt
    30721 cttataacta atctgctctt cgattttagc attaaagggg aaataaacca attttacaga
    30781 atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg
    30841 tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca
    30901 tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct
    30961 ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt
    31021 ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga
    31081 aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt
    31141 cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata
    31201 ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca aatataattt
    31261 acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc
    31321 gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga
    31381 ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc
    31441 cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat
    31501 tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa
    31561 aaagagcatg atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg
    31621 cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg
    31681 cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt
    31741 cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac
    31801 gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact
    31861 atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc
    31921 cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata
    31981 cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg
    32041 ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag
    32101 acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt
    32161 attaaaactg cctcct
//
LOCUS       DANJJH010000025        31459 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000025 DANJJH010000000
VERSION     DANJJH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            212..604
                     /locus_tag="PWS53_001062"
     CDS             212..604
                     /locus_tag="PWS53_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0871321.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWS53_001063"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWS53_001063"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL0871322.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWS53_001064"
     CDS             3165..4775
                     /locus_tag="PWS53_001064"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL0871323.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWS53_001065"
     CDS             5067..5936
                     /locus_tag="PWS53_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL0871324.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWS53_001066"
     CDS             6101..7372
                     /locus_tag="PWS53_001066"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0871325.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWS53_001067"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWS53_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL0871326.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWS53_001068"
     CDS             8763..9026
                     /locus_tag="PWS53_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL0871327.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWS53_001069"
     CDS             9471..9920
                     /locus_tag="PWS53_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871328.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWS53_001070"
     CDS             9977..11395
                     /locus_tag="PWS53_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0871329.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWS53_001071"
     CDS             11395..12255
                     /locus_tag="PWS53_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL0871330.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWS53_001072"
     CDS             12429..13202
                     /locus_tag="PWS53_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0871331.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWS53_001073"
     CDS             13349..13714
                     /locus_tag="PWS53_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL0871332.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWS53_001074"
     CDS             complement(13862..15070)
                     /locus_tag="PWS53_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871333.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWS53_001075"
     CDS             complement(15089..15367)
                     /locus_tag="PWS53_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871334.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWS53_001076"
     CDS             15521..16216
                     /locus_tag="PWS53_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0871335.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWS53_001077"
     CDS             complement(16658..17455)
                     /locus_tag="PWS53_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL0871336.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWS53_001078"
     CDS             complement(17452..18636)
                     /locus_tag="PWS53_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0871337.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWS53_001079"
     CDS             complement(18661..19908)
                     /locus_tag="PWS53_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0871338.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWS53_001080"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWS53_001080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL0871339.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWS53_001081"
     CDS             21888..22202
                     /locus_tag="PWS53_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL0871340.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWS53_001082"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWS53_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL0871341.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWS53_001083"
     CDS             22887..23531
                     /locus_tag="PWS53_001083"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL0871342.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWS53_001084"
     CDS             23546..23875
                     /locus_tag="PWS53_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL0871343.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWS53_001085"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWS53_001085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL0871344.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWS53_001086"
     CDS             24863..25687
                     /locus_tag="PWS53_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL0871345.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWS53_001087"
     CDS             25680..26027
                     /locus_tag="PWS53_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL0871346.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWS53_001088"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWS53_001088"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL0871347.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PWS53_001089"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PWS53_001089"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL0871348.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWS53_001090"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWS53_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL0871349.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWS53_001091"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWS53_001091"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL0871350.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10114 a         5635 c         6634 g         9076 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataa
//
LOCUS       DANJJH010000026        31023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000026 DANJJH010000000
VERSION     DANJJH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(16..951)
                     /gene="murB"
                     /locus_tag="PWS53_001092"
     CDS             complement(16..951)
                     /gene="murB"
                     /locus_tag="PWS53_001092"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL0871351.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1024..1944)
                     /locus_tag="PWS53_001093"
     CDS             complement(1024..1944)
                     /locus_tag="PWS53_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0871352.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2057..2809
                     /gene="xth"
                     /locus_tag="PWS53_001094"
     CDS             2057..2809
                     /gene="xth"
                     /locus_tag="PWS53_001094"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL0871353.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3673..4206
                     /locus_tag="PWS53_001095"
     CDS             3673..4206
                     /locus_tag="PWS53_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL0871354.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4696..5220)
                     /locus_tag="PWS53_001096"
     CDS             complement(4696..5220)
                     /locus_tag="PWS53_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871355.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5213..5686)
                     /gene="tsaE"
                     /locus_tag="PWS53_001097"
     CDS             complement(5213..5686)
                     /gene="tsaE"
                     /locus_tag="PWS53_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL0871356.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5809..6789)
                     /gene="pta"
                     /locus_tag="PWS53_001098"
     CDS             complement(5809..6789)
                     /gene="pta"
                     /locus_tag="PWS53_001098"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL0871357.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6942..7622)
                     /locus_tag="PWS53_001099"
     CDS             complement(6942..7622)
                     /locus_tag="PWS53_001099"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0871358.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7748..8617
                     /locus_tag="PWS53_001100"
     CDS             7748..8617
                     /locus_tag="PWS53_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0871359.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8643..9158
                     /locus_tag="PWS53_001101"
     CDS             8643..9158
                     /locus_tag="PWS53_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871360.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9205..9798)
                     /locus_tag="PWS53_001102"
     CDS             complement(9205..9798)
                     /locus_tag="PWS53_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871361.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9823..10002)
                     /locus_tag="PWS53_001103"
     CDS             complement(9823..10002)
                     /locus_tag="PWS53_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871362.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10079..10588)
                     /locus_tag="PWS53_001104"
     CDS             complement(10079..10588)
                     /locus_tag="PWS53_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871363.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10684..11064)
                     /locus_tag="PWS53_001105"
     CDS             complement(10684..11064)
                     /locus_tag="PWS53_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0871364.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11394..12143
                     /locus_tag="PWS53_001106"
     CDS             11394..12143
                     /locus_tag="PWS53_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL0871365.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12327..13604)
                     /locus_tag="PWS53_001107"
     CDS             complement(12327..13604)
                     /locus_tag="PWS53_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871366.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13683..14327)
                     /gene="pcp"
                     /locus_tag="PWS53_001108"
     CDS             complement(13683..14327)
                     /gene="pcp"
                     /locus_tag="PWS53_001108"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL0871367.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14526..15470)
                     /locus_tag="PWS53_001109"
     CDS             complement(14526..15470)
                     /locus_tag="PWS53_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL0871368.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15472..16158)
                     /locus_tag="PWS53_001110"
     CDS             complement(15472..16158)
                     /locus_tag="PWS53_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL0871369.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            16632..16952
                     /locus_tag="PWS53_001111"
     CDS             16632..16952
                     /locus_tag="PWS53_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL0871370.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17106..18011
                     /locus_tag="PWS53_001112"
     CDS             17106..18011
                     /locus_tag="PWS53_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871371.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            18008..18781
                     /locus_tag="PWS53_001113"
     CDS             18008..18781
                     /locus_tag="PWS53_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0871372.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(18962..20386)
                     /locus_tag="PWS53_001114"
     CDS             complement(18962..20386)
                     /locus_tag="PWS53_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0871373.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            20597..21043
                     /gene="nrdI"
                     /locus_tag="PWS53_001115"
     CDS             20597..21043
                     /gene="nrdI"
                     /locus_tag="PWS53_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0871374.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(21108..23090)
                     /locus_tag="PWS53_001116"
     CDS             complement(21108..23090)
                     /locus_tag="PWS53_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0871375.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(23104..24000)
                     /gene="murQ"
                     /locus_tag="PWS53_001117"
     CDS             complement(23104..24000)
                     /gene="murQ"
                     /locus_tag="PWS53_001117"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0871376.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(24003..25064)
                     /locus_tag="PWS53_001118"
     CDS             complement(24003..25064)
                     /locus_tag="PWS53_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0871377.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(25275..25841)
                     /locus_tag="PWS53_001119"
     CDS             complement(25275..25841)
                     /locus_tag="PWS53_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0871378.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(25869..27371)
                     /gene="eat(A)"
                     /locus_tag="PWS53_001120"
     CDS             complement(25869..27371)
                     /gene="eat(A)"
                     /locus_tag="PWS53_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0871379.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(27629..28750)
                     /locus_tag="PWS53_001121"
     CDS             complement(27629..28750)
                     /locus_tag="PWS53_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0871380.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            28876..29817
                     /locus_tag="PWS53_001122"
     CDS             28876..29817
                     /locus_tag="PWS53_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0871381.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(29967..30863)
                     /locus_tag="PWS53_001123"
     CDS             complement(29967..30863)
                     /locus_tag="PWS53_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0871382.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         8985 a         6480 c         5491 g        10067 t
ORIGIN      
        1 aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg cttgtttttc
       61 tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga ttacttcttg
      121 gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat tgacgatgaa
      181 tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca atcctgcttg
      241 ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc cgcatgaagg
      301 gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc ttgctttgat
      361 tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag cacgtaattc
      421 ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag ttttcaatgt
      481 tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc cgccatacgc
      541 acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag caaattcaaa
      601 tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg cacctgcttc
      661 cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca tgatgactac
      721 accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata ccatccaagg
      781 tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt ttttcgggaa
      841 tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca atggttcatc
      901 aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca ctgaggattt
      961 tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc ttgccagtct
     1021 tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt catgacttct
     1081 tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga tctgagaaaa
     1141 gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc agtcgccaaa
     1201 ttctttcact ttttttccat cagcctcttg aatgacaagc tgggtcagat cgtttacttc
     1261 ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac ttgctaaatc
     1321 catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat attccaaaaa
     1381 ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta ataaatacac
     1441 agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg tttcttttgc
     1501 cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt tcaattcttc
     1561 taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt tcttatcagc
     1621 taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt caatataaac
     1681 atggatacca ttttctagac attttttggc taattcataa tgtacttttg ttgctgcgtg
     1741 gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat atagatgttg
     1801 gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg caaaataaaa
     1861 atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg cgatattccc
     1921 tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt actttcatct
     1981 tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga aattaattcg
     2041 aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc gtgccatcgt
     2101 aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct gtctgcagga
     2161 aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc aatattggaa
     2221 ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac cagcattgaa
     2281 tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga ttactttgga
     2341 atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg aattaaaacg
     2401 tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa atctaaaaaa
     2461 acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga ttgatttgaa
     2521 aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag cggctctttc
     2581 tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa cccaggaagg
     2641 tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg gttggcggat
     2701 cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa agatccatac
     2761 ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa gaggttgtga
     2821 aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttg
     2881 cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg agagattgtg
     2941 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt
     3001 tcgtttggtt gggcttatta ccgcagatac aagcttttga acaccgttta ttaagaggtt
     3061 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     3121 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttattaagag
     3181 gttgtgaaaa agctgcactg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     3241 tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga
     3301 gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc
     3361 tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
     3421 gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc
     3481 ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca
     3541 aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct
     3601 atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga
     3661 ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat
     3721 agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat
     3781 acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat
     3841 caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat
     3901 tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg
     3961 ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa
     4021 agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat
     4081 ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa
     4141 atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata
     4201 aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag
     4261 tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac
     4321 cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc
     4381 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat
     4441 taccgcagat gcaagctttt ggacaccgtt taccaagagg ttgtgaaatc agcgttttga
     4501 cctgagtatt agagaaacaa acggcaaaat agttcctcat attttgccgt tttttgagct
     4561 aataccgaag gtcagctgat tgaacaccgt ttattcaaaa aaaatggact gcgacacgac
     4621 ttttgtcaca gtcccttcgt gtgtttttct gccttctcaa aaggatgaca gtttctcact
     4681 cttttcctat ttcttctatt caatcaaata actcatttta tatacatcta aaaattcacc
     4741 tagatctgtt cttgcccctc taggcatgac cgcttcgatt tggaagccca tttttcggta
     4801 aaggtgtacg gctctttcat tacgaacttg aacgtcaagc tccaatcgaa aaagtacacc
     4861 catttccttc gcccaactga tgatttcttc tagcataaga gtgcctagtc ccattcccca
     4921 atattcctgc agaatactga taccgacttc tcccacgtga gacaaacgaa attgactgtc
     4981 tgccttgaca gatgctgttc caataatcgt cccttcatag attgcaagca gcaataaatt
     5041 attattgctt tcgtatatat aatccaactc ttcttctaag gtttctggtg gtaacaggag
     5101 ttctgcttca tccaaaacta aaaattctgt ctccttatta acttgtccca tcatcaaaag
     5161 aatgtctttt gcatctcctg gcactgcttc ccgcaagatg atcccaactt cttcactcat
     5221 gattttcagc ccatttttct agaattcgct gtttgaaggt ttcactttgc tccccgtatg
     5281 agattagttt cacataccgc ttttctaaat ctgtatcact tttttctaaa atcagttcca
     5341 aataatcctc cggcagtgct tcttctaata gatttcccca ttcaacaacg gaaagcccat
     5401 caccttcaaa atattcatct aaccctaaat ctgctccgct agctgctacc cgataaatat
     5461 ccatatgata caacggaatc cgaccttgac tatattctct aataatcgtg tatgtcggac
     5521 ttttgatcat ttgcgttatc cctaatcctt gggcaattcc tttcgtcaac gttgttttac
     5581 ccgcacctag atcaccagtc aatatcagat tgtcacctgg cattgcggaa tgaccaattg
     5641 cctctgcgaa ttgttctgtc attgttaatc ctgatagttc gatcattttt catcttctcc
     5701 cgctcttctt tctttttgcc agtatatcgc gtaatgaagt ttttttaaag aaaaaatgaa
     5761 ctagtcaaga acagcagctg ctgtttttgg ctagttcgcg tgtatttgtt aatcaatcaa
     5821 tgtttgtgct gctgtgataa tcgataattt atatacatct tcttcatttg ctccacgaga
     5881 caaatcagaa accggtttgt tcaagccttg tagaattggt ccgattgctt caaagttacc
     5941 gaaacgctga gcaattttat agccgatatt tcctgactgc aattctggaa atacaaagac
     6001 tgttgcttga ccagctactt ctgaatctgg cgctttcagt tgtgcaactg aaggaacata
     6061 agaagcatca aattgcaatt cgccgtcgat catgtattgt ggcgctagtt ttttagcgat
     6121 tttagttgct tcaactactt tgtctacttc tggcgatttc gctgagcctt ttgtcgagaa
     6181 gctcatcatt gctacttttg gttcgatatc gaacaattct gctgtttttg cagaatctac
     6241 ggcgatttca gccaattctt gcgcagttgg attcacgttg atcgcacaat ccgaaaagac
     6301 atatttttct tgatctcttc cacgtaccat gatcatcgct ccgcttgtcc gactgatgcc
     6361 aggctttgtt ttgatgatct gaagtgctgg gcgaacagtg tcgccagtcg aatggatcgc
     6421 tccagagacc attccgtctg caatccccat atacgtaagc atcgtaccaa agtaattcac
     6481 atcttttaaa atcttttctg cttgttcttt ggatgccttt ccatttctac gttcaacaaa
     6541 agtatccacc atttcatccc acttatcgta attatcaggg ctgatgatcg taaagctaga
     6601 tgttttgatt cctctggcat gcgctgcatt tcttatttct tcttcgttcc ctactaaaat
     6661 gggttccaca agttcttctg ctttcaaccg ggcagctgcg cctaaaatac gagcatcagt
     6721 agcttccggg aaaacgattt tgatcccacg gcgaacaatt ttaaatctta aactatcaaa
     6781 caattccaca agatgacctc cagacgtttt tatatgtatt atcatacact attccagaaa
     6841 aaaagaacag tttattttat ctgtatcagt caaaaatgct ggaaaaatta agtttttcac
     6901 atacaaagcg aactgcacta taacaggaaa gcgctgtttt attacactgt ttcaggcagt
     6961 tgccaatcaa tcggttcttc tcctaacgca aggagcgcat cattcgtttt cgagaagggt
     7021 ttggaaccaa aaaagccacg acttgcagat aacggactgg gatgtggaga tgttatgatg
     7081 atatggcgac tcttatcgat cattttgatt ttttcttgtg ccggttttcc ccacaagata
     7141 aacacgattg gtttttcccg ttcatttaat ttttcaatga tcgtgtctgt taatctttcc
     7201 caacctttcc cgcgatgaga atacgcttgc ccttcccgaa cggtaagaac ggtgttgagt
     7261 aaaaggactc cctgtttagc ccatgacact agatttccat gttgaacagg tgcgattcca
     7321 agatcatcat aaagctcttt atagatattt cttaacgatg gcgggatctt tacgccaggt
     7381 tgcacagaaa aagataagcc atgagcttga ttttctccat gataaggatc ttgtcctaaa
     7441 atcaccactt taacttcttc ataaggagtc aattccaatg cttcatagat atgatacata
     7501 tctggatgga tcttttggga cgcatattct ttttttaaaa attctcttag atgcagataa
     7561 taatcttttg aaaattctga ggacaaaact tcttgccaac tgttatgaat gatttctttc
     7621 aaaaactcca cctcctaata aaaagaataa ctcacatttt cttcaatcac aagggacaat
     7681 cttcaccctc agcacttgat taatatggta aactaggaac aagaagcaga aaggaacgat
     7741 aatcattatg atcaaattaa ttgcatcaga tatggacggc acattattag atgcccatat
     7801 gagtgtttct cctgaaaatg ccgaggcgat acgttttgca aatgatgccg gtgttgagtt
     7861 catggtcgct actggtcgaa actatcaaga agctcgcgca gctttagatg aggtaggaat
     7921 cgattgtgcg atgatcactt tgaacggggc acaggtattt gataaagaag gaaattcatt
     7981 gtttaccgtg tcgattccaa ataatcaagc aatctctgtt ttagatattc tagatgctaa
     8041 cggtatttac tatgaagtag caaccaacga tggtctttat tctgaaaatc aagcgaaacg
     8101 aattgaaagt tttgcttcca tggtggctac ccatttacct caccttacgt ataaaatggc
     8161 gattgccatg gcctcagcta agctggagtt attgcacatc acatacgtgg acagtattcg
     8221 tgctattttg gaagaaaaga atctggaagt tttaaaaatt atttgttttc atacagaagg
     8281 gccagctgta ttaggtccag ttggaaagca gatttctctt ttaggcgatc tagccgttac
     8341 ttcctctggt caaaacaata tcgaagtgaa ccataaaaac gctcaaaaag gaattgctgt
     8401 tgcccatgtc gctcacgagc gtgggatttc tttagaagaa gtcatgacga ttggcgataa
     8461 cttcaatgat gtcagcatgc tgcaagtagc aggagtcagt ttcgcaatgg gaaatgcaga
     8521 aatcgaagta aaagattatg ccaaatatac aacggacaca aaccttgaat ctggcgtagg
     8581 aaaagccata ttgcgcgcga ttgcagaaga cttgtagttt tatctagaaa agagggatat
     8641 agatgtcaga atttttcatt caggaaaagc agcttggacg tgcaactcgg acaattgtca
     8701 aaaacgaaaa aggacattcc ctgtttttga tggttggtcg ctggggaaca aagggcgatg
     8761 cattgtctct ttatgccatg aatggcgatc tcgttgctca catcaagcag ataagcctga
     8821 cattcggtac gcgttttgag ctgtataaag agttcgaaaa ggtgggaacc atgcagaaaa
     8881 tattcaattg gcccggtgac ttttattata ttcgtagact tcactggaca gtccaaggtg
     8941 atatctataa tcatcaatat caaatccatc atttcaatca gcaaatcatg aaaatggata
     9001 aagcaacact tttttcagga gattactatg tattggatgt ctcaaatgat gaagatgctc
     9061 cagtttgtat ttgtattgct gcggttttag attactggct ctaccgtaaa gacaaaagtc
     9121 gcgataagcc tgtttcttac cgacttaact taagttaaaa agatgtacct gaaatgcgga
     9181 aatacgcatt tcaggtacat ctttttagat attcttttgc atatgatcat aaagatgacc
     9241 actgatcatc cgctgaccga caactttgaa gtcatggcgt tcgtataatt ttttagcctt
     9301 tggatttttt tcatccacac ttagaccgat gacggagcga ttagctttct tcgccaattt
     9361 aggtaatgct tctaataatc gcgaaccgat gccttgtcct ctaaaatctt ctgatacact
     9421 gatagagtcc aaataccatt cgttgggaaa cgtttcttta tcaatgaaca aacgtgtaga
     9481 cgcatctagc tgatatttct ctaagatagg taccagtggc tgatcgatga ttgattcttc
     9541 ttcatctgta taaccgtaag caactcctgc aactttaccg tctatttctt ctacgatacc
     9601 tcgtgaataa ctgtaacgat aatttggatc agtgattgct tcttctaaaa tctctaaagt
     9661 cttttgttcc cctgtctgct tcaaaaatgg cagttccata tcttttagaa tcactaagat
     9721 caatggggcg atttctggag catcttcttt tctcgcaaaa cgaatcatag tttcctctcc
     9781 ttttctccat tctaacatgc agaaaaagaa atcgcaaaat cgttatttta aaaaatcttc
     9841 aatcttttca gtaatgaaat aaccatgagt aacgattctt gccgcttcag ttcggcaata
     9901 agggcagacg actggcattt ctttatcatt atgttcgact acattattga cttcttcgta
     9961 tgcaaatttt tttccgcaat tagggcattt cattttttcc atgttatctc aacttccttt
    10021 catctttact ctcttagctt actcttatat tgtaaaaaag agaagcaata tccttcttct
    10081 atgcagatat ccatacctga cggtcctgtt ctccatctat taatcggact ttataataag
    10141 gacgttcatc tttgactaga agtgtccact ctttcacttt tgcttttttc ccgtagtagc
    10201 tgactgcttc cttcatcgct gcttgcggtt gtttgaactc ttccgttttt tctatatcaa
    10261 aaagatgttc tgcggccttt tccgtgtcat ttcgtacggt tgttttccca gtggatggat
    10321 tgactgctat gacttgtcct tcacaaaaaa actggtattc atatgttgca ttcgtaggat
    10381 tctccaaatc tttttcaact gcaaaatgaa tagcagatat gttatgttct tttgtttcgt
    10441 tttttacgat tgctgctgct tttttcaatg agatccgata ttcctggtca ctaatatcat
    10501 ccatgtgaaa agcggcattg agcagtcccg tcataccggt aatccctatt acgatgaggg
    10561 cagcaacacc aaacatcatt tttttcattc ttcattctcc tgtttctttt ttttaaagaa
    10621 tatcaagaaa tgaacgaatg aaaaatcgaa tgaaacgaaa aaaaagtgac aattttgtta
    10681 tctttattcg tgcagttcta atggcaatcc gtctggatca aagaaaaacg tcattttttt
    10741 tcctgtaaag gtatcttctc taatcggttc tgattcgatc cctaaggact gcaattctgc
    10801 aattgtttct tcaatatcgt ctactttgaa agcaagatga cgcaagccac aagcttctgg
    10861 atagtttggt cgttgtggcg cattagcaat tccaaaaatc tccaattcag cacttcctag
    10921 tttcagatct aatttatagt catctcgttc tttgcggtaa ttttctctga ttacttcaaa
    10981 tcctaattta ttaacataaa aatcacgtga tttctgataa tctgaaacaa tgatggctat
    11041 atgatggatc gttgtaaggt tcatatgagc actcctattc ttcgtttaag ctcggatcta
    11101 tattaatctc cagttccatt gtaaatgtgt cagattgaaa aaaatagctg tttttccttt
    11161 ttataagaaa aaaggcaaaa aaaacagaaa gtaggtcctc caagacactc ttcctgttcc
    11221 caatataata aatgaaagta agaaattata ttttcaagaa aacaaacaga aaattatcat
    11281 aacacaaatt atttataatt gaaagactag ttttttttca agaaaagcat acaatagatt
    11341 gtgttagtaa atacagaaca cactacattt agtgtcttaa ggagtcctca tacatgaagt
    11401 catattttca gtttttagct catcagctaa aaggctggcc gcaacaaaat tattatcttt
    11461 tctttttcag tcttggctgc caggtcatga ctcttgtcaa tagtcccatc acttggctgt
    11521 ccgttattac atttatcggt accacgcttg gtgtattgtg tattttatcc atcaatgcgg
    11581 caaaatcagt caacggtatt ttaggtattt tgtctgcgat ttgttttatt atcgttgggt
    11641 tttcagcaaa gaactattta agtatcggtg aacagattgc ttatatgatc acattggata
    11701 tccccgtatt gttaagtaaa gaatggaatc ataatatggc ttcaaaaatc cgaaaattca
    11761 ctggaaaaag ctggtttatc gcaatcatca gtactttcct tgtctatctt gtttcaggat
    11821 tcctgattgg tcgttataca gatgatcctc ggccgtgggt agatgccatt gctttttcta
    11881 ttagcttgac tgctggtatc atcagtttct tgcgttacaa caaccaatat tactggtggt
    11941 tagcttcagg gcttgctcaa atggtccttt ggtatatttc atttacgcat ggatctgcaa
    12001 gtttggcgat gttcgtgaac agctctgttt acttgctgaa tgatgtcctt gcatttaccg
    12061 tttcaccttg gtataaccaa aaagaacgtg aacgtatgca aaaaatggaa gaagaatact
    12121 acaacgcaca acaacacgca taaaaaaact gagcgtggga caaaagtaaa gaacactttt
    12181 gtctcacgct ctaaacacgt ataaacggcg gaatcagaag caactccttc ggaaataagc
    12241 tgaaattcac aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta
    12301 aacgcttctg tcccagcctc tattttttat agtaattcct tcagatactt tcttgcctca
    12361 tcattgaaac gcagtgattc ttctaacttc tgataatatt ccaacgcttg tttctgagct
    12421 tcagtaaaac gataatgttt tcctttccat ttgtcgattg tctcttgcac atttccagat
    12481 tggatatctt tcactactag ttgccatttt tcttttgaga ctgtacggtc tttcttatca
    12541 gcagattgta tagcttctcg ttctttacgc aatactttta aaaaaccatt tctgactatc
    12601 cgaggaatgc tgtcgaagaa cccttctgta aaaccatagc gacaatattg cttccaaata
    12661 aatagataga tttttggttg gatgaatcgt tggtttttcg caaaatattc catctgagca
    12721 gtgacttctg gtgttcgttg acgttttgtc tttctcaaca cggaaataaa tgtttcaaaa
    12781 taagcattag gatgtactgg cacttcttca tatacgatgg attgccaatc tttacgtaaa
    12841 aatgcgcgat agttgatcaa gaattcttct acatccttta ctaattcttc tgcaaagtcg
    12901 tcaaatagtt ctttttttat ctgtcgcttt tttgactgcc cattgatgtg ttcacttatt
    12961 ttcttgattt cattttccaa tgcttgataa tccgctatgt aaatcggcat ttctacttgg
    13021 cgaaattctg cgagccgctg agctaatttt atatccggta aatatggctg tttcatccga
    13081 cgattttggt acaagacaaa acagtatttt tgccatagtc cttcaggaaa atcgatattc
    13141 ttctgcttaa gccataataa ttcgtctaag gccagatcaa cttttgtcat tccctgatgg
    13201 atcgaagctg ccactgtcgg agagacatgc aggtgatcgg caaagtggtt gatgcctagt
    13261 tttaggatct ttccggtttt tggcgattta acgatgtatt gcacttttaa ttctctacca
    13321 cactcacaat acaatttttg ctggcttcgt tttgttcgaa aaaacggatt gatcttgaag
    13381 tcagaaaaca cccaatctgt tgcagctaaa taatgttctt ccataaaacg agagctgatt
    13441 agatatcggc ggtgcttatc gactagttcc cgctgttttt tcgttaattt tgcatagatc
    13501 tcccaacgtt gactgcttgt gtattgttta gaaattttga tgggatagct tttgctgatt
    13561 tcttttggaa accattcttc aaagacggat tgccattctt ccacggctgt ttctttcctt
    13621 tctaattaca aaaagctggc tactaacata acgttagcaa ccagccagtg tttcttgcta
    13681 aattagtgag ttgttccggc atttccggaa atatcttctt tattttctac tgctgcttca
    13741 actgcataag tcaaagaaga agcaatgtct tctaaagaca tagaaggcgt acccactgga
    13801 cgatcgatca cttgatcggg tgagaatggc acgtggatga acccacctcg aattgccggg
    13861 aattgtgttt cgatcaaatg aagcaaacga tacatgatat cattgcagac aaatgttcct
    13921 gctgtataag agatgtacgc tggtagacca tgatcttgta cattttttgt cattgctttg
    13981 attggcaatg aggtaaagta agcggctgga ccgttttctt ctaaagccgt atcgaatggc
    14041 tgatttcctt cattatcagg gattcttgct tctgctagat tgattgcgac acgttcgaac
    14101 gagactgctg accgcccacc tgcttgtccg acacagatca cgacatctgg ttggtgctgt
    14161 tggatggctt ctttgactac ttgactgctt ttttcgaata cggtcggtac ttccactttg
    14221 atgatttcag caccgctgat ttcatctggc atttttttta ctgcttcata ggctggattc
    14281 actttgtccc caccaaatgg atcaaaacct gttactagta ctttcatctg ttgttcctcc
    14341 gtccgattga tttaacatat ggttatcttt tatcatacca ttttccttga acagcgtaaa
    14401 taaaggaaac aatagtttaa ataacataac agatcaaact taatatattc ttataaaaaa
    14461 atagtgttcc actacaatgt tagaagaggt tgcgacaatt attctgtcac aacccctctc
    14521 ttctattatt taaatagaat catataagca atttggaaaa ctagcataaa taacgcaata
    14581 aacaactggt ttttgatcac tccgtatttg tctttcatct ccagcatcgc tacaggcacg
    14641 atattgaagt tagcagccat cggtgtcagc agggtcccac aaaaaccgca agtcaatgca
    14701 accattccta ttaacgctgg attcgctcca tgactaaata cgaatggtgc accgatccca
    14761 accgtcatca cggtgatggc cgcaaatgca ttccccatga ttaccgtaaa tatcaccatg
    14821 ccaagagcgt aaatgatgat gccgatgttc acatttcctt ctggaacgat cgtacctact
    14881 gcattggaaa tcactgtccc aactccagcg cttgtaaata ccgcacctag agaagctaaa
    14941 agcattggca acatgcttaa cggccctact gtgcccagca tatctgctgc atcatctaaa
    15001 aatgtacttg gtcgattatc tcgagaaaag aacatcaaga ttagcatggc tgcaaaaaca
    15061 ccgacgccca cgccgactaa agcacctaaa ttggtaaata gagcaaacaa gatcgcaaag
    15121 acaccgatac tcaaagctgg aataaaaatt ttcattccta gtttgtctga cattttttcc
    15181 atatattctt ttgatggaag ccggctttct cctttgctga ctttcttagc aattgctggt
    15241 atggtcatcg caaagatcaa gccaccgttc acgattgccg gaagccatct tcctcctgca
    15301 ataactaccc ctaagaccgt ccagaaaaag gctgacccat aacgatgagg attggtttta
    15361 tctgttaaat ttcttactcc agtgtaaatc aatacgagtc ccatcacgat ataaaggatc
    15421 tccagtaatt tatcgcctaa taaaatatca ggatttgtaa aaaagtccat cttattgccc
    15481 ctttcctgga taatatttct tcgctaattt cttttcttta agatagaagt agacacttgc
    15541 cacaactaaa gccgtgatag ccactggaac ttccactaaa gcaagctgtc ccaatgttac
    15601 gtcatatcct agatctttca gtgttgattg gactagcaac gcccctgctc ctccgatgaa
    15661 tagtacttgg ccaaagaacc agcagacatt ttccatagct gaagccatac cttttagttc
    15721 ttcttcatgc ttaggatgga cttctaaccc ttttgattcg atggtcccca atgccatcgg
    15781 taaaatgatt ggacgaacaa atccagcaac tccaccaaaa ctcacgttaa aagccgcaaa
    15841 gatcattcga aagatcccat aaatatctac aatcatacca gcagatactt tcttgaatcg
    15901 cttgatcaag gaagccgctg attctttcag accatttctt tcaagcgttc ctgttgccaa
    15961 cataatgatg ataaaaatcg ccatcccgcg attgtttaca aaacttgtac ctaaaagttc
    16021 taacattcct tgaatgccaa gaccgccagt caaagctgta acaactaaag caaccattac
    16081 gatcaatatc gagtctaatt taaatgcaaa cccgagaata acaatcaaga cgcctaaaag
    16141 gctaagataa ttttccatat attttcctcc ataaaatgac aatatttttt taattataaa
    16201 gcaaaacaca taaaaaatat agttgttttc tcacgaaata gacactattt ttaaatattt
    16261 ttcgttattt ttttatcaca aaaattatct tacttattgt aaggtaaagc tttttttaat
    16321 ttattgcccg ataatatata gaaataaggt ctagttactg ttttttttaa atgattaata
    16381 aataacaact tctttctatg aaattgcaaa gcattcatct tcttttccgc taacgatata
    16441 tccgtcagtg cttggtacac tttactaaaa agtatgagat tgccgatatc tggataggtg
    16501 tgctatacta cagaaaaaga tatacaatct gccaccgggt aggataaaga ggatgatcat
    16561 tccattaggt ctttgaaggg atgattttcc tctttttgtg tattttttta tagttaagga
    16621 ggaatgtaag tatggcatgg atggaattaa tcatagcagg aattttggaa gtattctggt
    16681 caacgatgat gaaatggagt gatggttttt ctaaaatcaa ttattctttt tacacagtga
    16741 ttggcatgat tgctagtttt tactttttgt caaaggcaat caaaagtttg ccgatgagtc
    16801 tctcttatcc tatctggaca gggattggcg cagttggttc ggtcataatt ggtgtggtct
    16861 tgtttcatga taagctgaat ctttccactt ggttctttgt ggggatgcta ctcatcagta
    16921 tcattggcat caagattact agcggccact agacacttgg aataagtact gatcagaaaa
    16981 aataagcctt tttatttaat attcaatttt tttatgaaaa aataagaaat aagtccaatg
    17041 acctagcgac ttctccactc atcaggtgta tgcttgtact atctatagaa aaggagtgaa
    17101 tatttttgaa aagtctttcg gtccaagaat tatccaagaa ctttggtcac aaacaagccc
    17161 tcagcgatgt cagttttagc ttatcccctg gcaaaatcgt tggattagta ggtcctaatg
    17221 gcgctggaaa aacaacgatc atgaaagcta ttttaggact gatccattac tcagatggaa
    17281 caatctgtat tggtgaaaat gagatttctc cctctagtca taaagggttg gaacaagtag
    17341 gagcattgat cgaatacccg ggtatctatc cattcttaac aggctatgat catctgaagt
    17401 tattctctga aaccaacgat gtatctgcta tcgacactat cgtgaatcag ctcaagatgg
    17461 aaaagtacat tcacaaaaaa gcgaaatcct actcgttggg aatgaaacaa aaattgggaa
    17521 ttgctcttgc cttactgaac catccgtcta tcgttatttt agatgagccg atgaacggtt
    17581 tggatccaca agcaacaaga gatgtgcgaa aaatcattac cgacttggct actcagggca
    17641 cctctttctt gatctccagt cacttactca gcgagcttga aaaaatggtc gatgagctga
    17701 tcctgatcaa tcaaggaaag atcatcaaac aatgtacaat ggctgaattg aaccaaacag
    17761 cgcaagattt catcgttatc tctacaacaa atgatctagc tgctcgaacg atcttgacag
    17821 aagctggata tactttggaa gatcctgata aaatcaagct tttaaaagaa aacgaagaat
    17881 tattaggaac cgtgatccag ctattgtctg cacaaaatat ccatgtgaca gacgtccagc
    17941 atctgacgaa tgatttggaa tcttctctat tggaaattct ggattcagta aagggggaag
    18001 aagaaaaatg acattgcttg taaaacaaga attatttaaa ttgatcaaga aaaagtcaac
    18061 tgctgtttta tctgttctat tagttgtttt gctcatagga acagcacttc tagccaaaaa
    18121 atacacgacg atcattgatc cagttgaaat gactgctcag ctttttagtg ccacatcttg
    18181 gattgtcttt atcatgattg cagctgcaag cacgatcatt tcgatggaag cacaatatgg
    18241 tacattgaaa aatctccttt atcgaaagta ttctcgagga gaaatattag taagcaaatg
    18301 gatcacatta gttatttatt ctgtttattt atacctttta gctattattg tcactgtgtt
    18361 gatgaaactg attcttttcc cttcgatttc cttcacggag aaggtttcta ctggtcaaac
    18421 actgatccaa agcttgttca cttatacttt gggtagttat atcggtttat ggctgatttt
    18481 aagtttagtg ttgatgattg cttgctttat caatagttcc ggcgcatcca tctcagcagg
    18541 aatcgtcttt tattttgctt cgtcaatcat atctggtatc cttattgcac tcatccaaaa
    18601 atgggagtgg atcaaatgga atccgatcag tatgttgaat ctgcaaaacc aaattggaaa
    18661 cgaagagatc atgaagcaat tgactcatct ttctactaat caaatgctat tcggtaattt
    18721 agcttacatc gttttgttct tagcattagg atatctcgta tttaagcgaa aaaatgtatg
    18781 atgactcatc aaaaaagagg ctgtgaaaga gacgttcagc cttattgccg attggctgtc
    18841 tcttaaacac cgtttattct gaaaaaggag ctgtgacaac atttgtgtca cagctccttt
    18901 cctagtatgt ttttgaataa acgacaggct cacaacctct ctttatactt cttttatact
    18961 tctatacgtg aagcatccca gtggcaaata aaaccattgc aatcactccg accatcgtca
    19021 cacctataag taaaatctta tctaagtgga tatgttgtac aggctcgttt tgctccttac
    19081 gtgctttgag gtaaaaataa atgcctggca agaaaagaac aagaacaagc attacttcat
    19141 taaaggctac gactagcata gcccatactt gaaaggcact tgctaaaagc cctactaaaa
    19201 attggaccca ttctttccgc tgataagaaa gtttcatttg gtacaatcct aagaaaagcc
    19261 acgtgaagaa aatagctgct gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga
    19321 ataataatga aagtaaaaag acattcgtac ataacgtcgt caatacgata gcataagttg
    19381 gtgccccatt tttgttgagt tttccccatg ctttaggcaa aagtccgtct tcagccatgt
    19441 ttcccattgt ttcaatcggt aagatcgtcc atgagagcca aacaccaact aaagaaatga
    19501 tcaaaccgcc gttgatcacg actgctcccc atttaccaac catcatttca aacacatagc
    19561 ccatagcagg ctgcgccttg atcgtttgca gctgattcat cgtcattgtc ccatatggaa
    19621 gcatggaaac caagacgtaa ataataatca agccgaatat ccctagaatc gttgctttcc
    19681 caacatctga ttgttttttt gctcgtgaac ctacgacaga ggctccttca ataccaacaa
    19741 atacccaaag catcaccatg atgcaactca atacttgact ggaaaacggg atcggctgtc
    19801 cattcgcatt caatataatg tcgctagaaa agttatcaat gaattgttgg atcgaaaacg
    19861 tgctgatagc tactacaata aataaaaata atggcactaa tttacagaat gtgacgatag
    19921 cattgacgaa actagcattt tcgactcccc gattcactag ccatgcatat ccccataaaa
    19981 agactgtact catcacgatc gatgccagat tttgcccacc ttcaaatact gggaagaaat
    20041 atcctaatgc gctcatcaac aatgttgcaa aagctacgtt gcctaaccag catgtgagcc
    20101 agtaacccca accgctgata aaaccagcga aacggccaaa accttcttct gcgtaactaa
    20161 agatcccgcc ttttaaatct ggacgttttt tattgatatt attgatcgac aaggagagca
    20221 tcaacattgc tgttcctacg agtacccaac tgaaaatagc tgggccgcca gccgatccgg
    20281 agatgtccgt cataatacca aatacgccac ctccaatcgc tgaaccaatt actaaggcag
    20341 caagtggaat taaaccaatt ccttttttct gttgctctgt atccatgtaa gcatcctcca
    20401 aaataaaacc gcttttatca aaaagtataa caaacctttt ttatttttac ttaattaata
    20461 aaaactttaa tagaaatata attctaccgg cttttttctc attatacaag caaagcgcct
    20521 agtccctttc caaattacac caccaaaaaa cttaaaaaag atgctatact aaaaggaaag
    20581 aattggagga tgatccatga acattttata tatatcgatt tcaggaaata cgcgtgcctt
    20641 tgccaaacat ctagcagaat atgctgaaaa aatgcatgca gaagatcctg tcaatcctga
    20701 agttacttta aaagaaatcc atgaaaatag tgattttacg aaagaaacag aaccattttt
    20761 tactttcgtt cctacttatt tagacggcgg aaatggttta gataacggtg atacagaaat
    20821 cttgaccgaa acgatgcgtg aatatctgga acatgaagac aatcataaac tttgtcttgg
    20881 tgtagtcggt agcggaaata aaaacttcaa taatcagtat tgtctaacag ccaaacaata
    20941 tgcacaaaca tttggctttc ctttcttggc agattatgaa ttacggggaa caccatctga
    21001 tgtgacacga atttatcaga ttttagcaga aaacaataaa taataaaaaa gaagaagctg
    21061 aaaaatagat ttcagtttct tctttcgttt ctattgtttc tatcatatta agctttgaca
    21121 tccatcacag gtgcttttgc ttttgcttta cctgtttttt ccaagacaaa gttttttaca
    21181 cgatccatat tggttaccaa gacgatcatg gatgtttctt tgcctgctgc tttgatcgct
    21241 tctaagtcag cttctgctac aagcgtatca ggtgtcactt gctgcccttc tgttactttc
    21301 acatcaaatg gcgtaccgcc taagtcaact gtgttgattc ccatatgaag caagatttct
    21361 aaaccatttg gcgttgtgat cccaatagcg tgtttcgttg ggaagacaga gctgactacc
    21421 ccagtaactg gagaatagat atgtccgttt tctggttcta caaagtagcc ttcacccatc
    21481 atcttttgtg caaaaactgg atcacttgct ttatctaaag aaacaactgt tccgtcagca
    21541 actgatgaga ggatagtcac actttcagct gaagtttgag gtactacttt ttcaggcact
    21601 tcatttactg tggctgtttc agtcgctaat tcttcttttt ctaaacggtc tttaggaata
    21661 ccgaagaaga atgtagcaac gaagccacca gcataagctg ccaataatcc aaggatgtag
    21721 cctaaccatt taccgtcaga gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg
    21781 atagccccga catttccgat cattccgatc actgccccgc cgatacctcc accgatacaa
    21841 gcagtaataa atgggcgtcc tagaggtaat gttactccgt aaatcaatgg ttcgccgatt
    21901 cctaggatcc cgactggtaa agctcctttg atcatttctg ttaattcttt atcttttttg
    21961 cagcgaatcc ataatgccaa tgctgcaccc acttgtcctg caccagccat cgcaagaatt
    22021 ggaagcaata gcgtacgacc agtttcatca atcatttgca catggattgg agtcaaaatt
    22081 tgatgcagtc cgaacataac catcggcaag aataatgtgc ctagaacgaa gccggcaaat
    22141 gctccaccaa cgccaagtac ccagttgatc ccaccgacca tgccatcaga aatgaaacct
    22201 gctaaaggca tgatgaagaa aatttcaatc agaccgatgg caatcaatga aagagttggt
    22261 gtaacgatga tatctaccgc atcaggtact actttatgca gttttttctc aacgattgac
    22321 aatagccaaa cagcgaagat gacacctagg atcccacctt gtccagctgc aagagcttca
    22381 ccagtaaata agtttttcag tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc
    22441 ccgataactc caccaagagt tggtgttgca ccaaaaactt gtgcagcatt gatacctgta
    22501 tagataacta ggtagctaaa catcccgttt ttcaagatgt tcatcacatc gatatattgt
    22561 tgccatgttg ctgcatcaag tgttccggct gtgactaagt ttgctaaaac tgcggcaatc
    22621 cctgccacga tcccagtacc gacaaatgca ggaatcattg gcacaaagat attagaaatg
    22681 tcttttaata ccgatttgat tttagatggt ttttgtttcg atttttgtgc cgctttcatt
    22741 tcttgtgccc gttcgtttac acggtctttt ccagatgctg atgaatttgc tgtagctgta
    22801 cttacagaag aaagtttttc tcctaatttc acgcctgcca aatcaaccat ttgctgtgct
    22861 actttgttca ctttcccagg tccgatgatg acttgaagct gttcatcttc tactacacca
    22921 agcactcctg gaatcgcttt taattcagag acatttacaa gatcatcatt gatgatcttc
    22981 atacgcacac gagtcatgca gtggacaatg ctttctacat tgcccatgcc gcctgcccct
    23041 ttataaattt gttttgccag ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc
    23101 ccctcataag gtccctttta caaatccgtt tgcttcagct aattttgctt gagcttcctc
    23161 tttatcacta tttgtcaaaa tcatgacgat agccagctta acattttgat ctgcttcttc
    23221 aaagtacttt tcagctgttt cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga
    23281 gcgttcgatt agtttttcat ttgttggttt gacatcgacc atcaagttat tgaatacttt
    23341 acctatacca atcatactga tagtagaaat catattcaag ataagttttt gtgctgttcc
    23401 tgacttcaat cgagtagagc ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa
    23461 atccgcgtgt ttactgattt cggcttgttt attgcaagaa atcgttcctg tgacagcacc
    23521 gatcgatttt gcgtaatcta atccaccgat cacatatggt gtacgaccgc tagctgcgat
    23581 accaataact atatcatttt ttgtcaaatt cagcttgatt agatcttctt gccctagctc
    23641 ataagaatct tcggcccctt cgacagctac agtcattgct tcttctccac cagcaattag
    23701 acctatgacc atttccggtt ctacaccgaa tgtcggtacg cattcagcag catctaacac
    23761 acctagtctt ccacttgttc cggctcccat ataaatcaga cgtccacctt ttttaaagga
    23821 ctcaatcgtg ttagcaatca ctggttcgat acttgtcagt tctttttcaa ccgcagcggc
    23881 cactttctga tcttctttgt tcattagttg aacggcttga gaaatactca tctcatctaa
    23941 tccaaaagtt tcttggtttc tgcgttccgt tgttaggttc tctaaattga ttttgctcat
    24001 ttttatcttc cttcttttct tataaattga tagttcatac cagctttgat ttggttgatc
    24061 aatggtagct cttctttgat aactttagct actacattca ctttttcatc cgctggcaaa
    24121 tcatatttgg ttaattggat ctcacccata tagcgcaaat acttttcgtt atctaaagta
    24181 atagaccctt tcatacgcgt agctgtattt cttgctggga taggcggaat ctctcgaaaa
    24241 cgtgcatcta cgcttcgtat gacatctctt gcatcgtctt gccgattcgt atgcttccct
    24301 aatatatatt cgtaaaattc ttcatctaca ggttccgtat ggagcaagac tgtctgttct
    24361 ttttggaaag aagcaaattg ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa
    24421 acaatatccg tgttacttgc taatagatct agagcgcctg caagaggatg gatccctcga
    24481 tgttcttcca aagttggcaa tccttgatat agtgggcctc tcaactctgt atctcctggt
    24541 acaaaacctt ggacaaggag accttgaatt ttgagccatt gatttttttt ctgataccat
    24601 tctttgtcca gccctgtttc gggacgtgga taataattat gccaagcttc aatctgtgag
    24661 aaatcagctt ctgcttcttt caattcatcg atatctttag gcgtgatcgt gctagcattc
    24721 aaactgattt tcaattgatg tgaccagtcg gcaatctgct gattggaaat atgataatcc
    24781 attcttaaac ctgttacacc aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc
    24841 gctcgttcca atgcctctcc tgaaatatcg accatcaatt ctagtttatt ctttttcgct
    24901 tcgcttccta gttcgatcaa tcgcttttga tatgccgaaa catcatcttc tggtatatgc
    24961 atggaagtga aaataccgac aaagccattg gatgccattt ggcggatata ctgtttcttc
    25021 tcttctgaca aagaatcgtt cataaatatt gaaaagccaa acatattatc cctctttttc
    25081 tgtatccgtc ttcatttcta tgactagagt atatatctaa agaaacaaaa tttcaatact
    25141 tttattaaaa taattaaatt tcatttctaa aggttgagtt tatattatca aaagaattgt
    25201 cataacctaa aaaaggcact gctaagcaaa agcagtgcct tgaaatattt tatttaaata
    25261 acacaaaaac ataattactc ataagaaacc aattctgtat tatctaccat gaatgtaaag
    25321 aacccctcat taggcatcgg atgaaaataa gcattgatca ctcttatcaa ggcattatgg
    25381 caaactaaca gatagacatt ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc
    25441 cgtgcataca catctaagac tgactcccca tttgggaatc gtacagcgaa cgctaaacgt
    25501 gctttttgaa agttctcatc tttgcttggc agtccatcat aatccccaaa atccatttct
    25561 accaatcgtt catcaacggt cagcggcaag ctgagacgat cagcaactgc ttgtgctgta
    25621 tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact
    25681 accttttctg ctagtttctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc
    25741 cgtccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc
    25801 atgtgattct ctccttcgtt ggcaaagttc cccttaacca taccttgttg ttcttgagat
    25861 tacaagggtt agattctctc aagagaaata atctcagtag atactttatc caggaaggtt
    25921 tgatcatgtt ccactagtaa catggcaggt ttcacgttca agatcagttg ttctaattgt
    25981 tcttgattga agacatccaa ataattcaat ggttcatccc atgtatatag ttcagctggc
    26041 tgtgataaag atttagccaa ttccactttt ttccgttggc ccatactcat ctgctcgatc
    26101 ttgttatgaa aaacatctct ttccatccca agctttcgga ggttgttcaa aaatgcttgg
    26161 tagtctactt ggtttttctc tgcaaattcc gccaacgttc ctcgattgtc ttcataattt
    26221 tggcttgcat aactaatgct cagatgtttt ggctggtatt tttctcctat aaccttgccg
    26281 ttgaatgccc ccagaagata atggataatg gatgactttc ctgcaccgtt aggacctgaa
    26341 atcgccaccc gctgatgagg ctcgattgta aaatgaattg gctcgaataa cagattttca
    26401 taccctaatt gaagatcttc tacgcttaaa agtcgcttat ggtgagacgc ttggctattc
    26461 atcgtcaaag aatcgatata ttcgatatct tttaatagtt tttctttctc gctgatctgg
    26521 gtctccatcc gattcacgat tgctttggat cgtttcatcg tccgtgcagc atcagcaccc
    26581 actgctcctt tattcactcg tctagattca gtatcgatga atccgacttg tttctttgtt
    26641 ttatctcctt ctcgggaacg agaccattcg gctttttcag ctgctgtttt ctttagccta
    26701 ctgacttctt ttttcaattt ttcattttga gccatttcaa attcatcacg aagttttttc
    26761 tgttcttcat agatagagaa attcccttga taaagttcca gttgactttt ttcgattgct
    26821 aaaacatggt ccactacttc atcgataaat ccccggtcat ggctgaccac gatgaagcct
    26881 tgttttttct ttttcaaata agccgctact tgttttctac cagagatatc caaatgattc
    26941 gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataaaagtgc cagtaggact
    27001 tttgtcttct ctccccccga tagtgtgctg aattctctcc ataagatttc aggatctgtt
    27061 tgcatcaatt ggagctctct ttcgatttcc catatctcaa aatccgtaat atcatttaac
    27121 acgtaatagg ttaaacgttc tttatctttt gtctgttgcg ggaaataggc aaattcttgc
    27181 tgatggatta cttgcccttg ataaggtagt ttgttttgta gaatattcag taaagttgtc
    27241 tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa atttagattt
    27301 gcttgttcaa ataataatgt gccttggctg tcgtagccga atgtcagatt ttttatttcg
    27361 attttagaca ttttccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta
    27421 aagaggatgc aagggttgga tacacacaaa aagacccata catcgaaaaa accaagccaa
    27481 tcccgaaaat ctgcacactg cttccttgtc tccacaaggt acagtctgaa tgcagctttc
    27541 atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac
    27601 gatagcataa agaaaatgat tttgtcaact acttcgggag gatggtgaaa aatctgctaa
    27661 gtgtctgtcc taagattttt gttttttcta atgtttgcct gtgttccatt gcttcttcta
    27721 aacctattgg acccaaatga atacttaaga caattgggag aaaatcagaa agtccttcta
    27781 gctcttggtc tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca
    27841 ctgctacacc gtatggaact tttccgtgga tcgtttgctg atcgatccgc ccttctcctg
    27901 taatgatgac atctgcttgt tcgatctttt tttccaatcc agtgatttgg ctgactaaat
    27961 cgaaaccaga aaccatcttc gctcccaaca aaaaaagggc tccaccaata ccgcctgctg
    28021 ctccacttcc tggaatatca tctaaatcca cctcccagaa atcttttatt tgctgagcaa
    28081 tctttcctgc ttgtttatct aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc
    28141 caaattgcca agcagctcct tgttgacctg tatatggatt ggtaacatct gcagcgataa
    28201 tcaattttac tttagaaaca agaacgtttc ttcgatcaaa atgaagtttt ttagtagata
    28261 ataaaggatt tccttctgtg tacccttcta aagaagcacc caaggattgg agtaatccta
    28321 gaccaccatc gctacacccg cttcctccta aagtgacaat aatttgttct acttcttgaa
    28381 gcagtgcatc tttaataagt tctcctaagg cataactgga agcaagtcgg atagtttgat
    28441 ccgttggttt atcaagtaga tctaacccca atgcttgtgc agattcgatg actgcggtgt
    28501 tttttccttg ccaagttgtc aataaatagc tgatttttcg ctgacggaat aagagatcca
    28561 tcccatcaat ggtacgccat gttcccctga gcgcgtaata gattgcttct gcagtatttt
    28621 ctccgccatc tgcaatcggc acattgattt ttcttccttt acgatcttcc cagccattta
    28681 gaactgcctg atttagttca aatgaagaag cacttccttt aaatgaatca attgccgcca
    28741 aaatatccat ctgtatccct cactttcttc ttaagtaaga cacttttttt catttttttc
    28801 aaacaaaaca atgctatttt cttccatttt tctatatact aaaagggaac tcattgaata
    28861 aaggagagaa aaaacatgaa aatcgcaata gctggggccg gagcaatggg cagccgaatc
    28921 ggattgatgc tacatcaaag cggaaatgag gtcctcttga tcgatcgttg gcctgctcac
    28981 attgaagcca ttcgtacaaa tgggttgatt gctgacttta acggtaaaga agtggtcgca
    29041 aaactgccaa tctattcacc agaagaaatc atcgaaagca acgaacacgt ggacttgatc
    29101 gtagcattaa caaaagccaa tcaattagat gatatgtttt gttcgatcca atccattatt
    29161 actgacaata cttatgtttt gtgtttacta aatggattag gtcatgaaga tgtactggaa
    29221 aaatatgtgc caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
    29281 ggtcccggaa aagtcacatt gttaggtgat ggagaaatcg aactagagaa cttggaacca
    29341 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa tttaaatccg
    29401 atctatagcc ataacgttcg ttattccatt tggcgaaaag cttgtgtaaa tggaacattg
    29461 aatggtttat gcacgatttt ggattgtaat attgctgaac taggcgcgca aaaagcggca
    29521 gagagcatgg ttcgaacaat cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc
    29581 atactagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
    29641 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
    29701 gactacatca atggtgccat ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
    29761 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
    29821 gctctctgat gagaatcagc tgaatcagca gctggttctc tttttttaag cccaataaaa
    29881 gatcaaaaag aaaacacaac tctcatgatg ccataagaat tgtgcttgtt tgtttcagta
    29941 cataatctct tcaaaatgta ggttttttat ttgttttcaa cagaggagag gtcatattcg
    30001 tattgctcat aaatagtgga atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca
    30061 taaaattcca atagctctga taggattggc ttaaatggtg tatgcagaaa ttccggtaat
    30121 tcatctaaat cacgttctcg aagcaaaaaa atattttcag gacgataaaa atgataatga
    30181 gcaatcgctt gattatccgt cactagtttc ttttgaaaat acatggattc aaatgtccgc
    30241 aaagtcactc cttgctgacc ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt
    30301 tttttcaggt actcttcata agtaagatat ccaggttgtt cattccctcg atgtggaaga
    30361 atgaaatagt tggtcgtaac atctcttttt ataaattcct gcttatagct ttctgctcgt
    30421 tcttttctcc cattattcgt tgcaccaaaa aatagatccg tctttggttc gctagaagaa
    30481 aaattcatat gtttcgaata aaatgaacta ttatgtttca gcccaagttc ttttgcttcc
    30541 aaaaaatcaa aatgataaat ctcatcgatt gccggttcat cttgtgcctg cttcagcatc
    30601 gcctcgtgtt ctgctttgaa gtgattccaa aaaaataaca caatttttgc attggtaaat
    30661 gggcgcacgc gtcgagccat ctccatatca aatccataat ctaaaaaaac gatcagctga
    30721 taatttgcca actgttgtct agctgattta atgaattccg actgtagtaa atcttttccg
    30781 gaccaaacat ctaaagagcg gatggaaggc aaaaaataaa gtgatttatt ttttgcaact
    30841 agcagtacgt catttttgtt catcttttcc gcctcacttt ctctgtcact ttttcctatg
    30901 atagcctgtt taaatagtaa aaagccagta aaacgtttcc tatatttgta tgaaaattta
    30961 acatttgtat gaaaatatga tgaaataaat cattcttcta ttaaaattcg cctaaaagtt
    31021 ttc
//
LOCUS       DANJJH010000027        30099 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000027 DANJJH010000000
VERSION     DANJJH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..106
                     /locus_tag="PWS53_001124"
     CDS             <1..106
                     /locus_tag="PWS53_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0871383.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            255..593
                     /locus_tag="PWS53_001125"
     CDS             255..593
                     /locus_tag="PWS53_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871384.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(798..1373)
                     /locus_tag="PWS53_001126"
     CDS             complement(798..1373)
                     /locus_tag="PWS53_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL0871385.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2105..2686)
                     /locus_tag="PWS53_001127"
     CDS             complement(2105..2686)
                     /locus_tag="PWS53_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0871386.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2869..3495)
                     /locus_tag="PWS53_001128"
     CDS             complement(2869..3495)
                     /locus_tag="PWS53_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0871387.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3517..4848)
                     /locus_tag="PWS53_001129"
     CDS             complement(3517..4848)
                     /locus_tag="PWS53_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL0871388.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5003..5404)
                     /locus_tag="PWS53_001130"
     CDS             complement(5003..5404)
                     /locus_tag="PWS53_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0871389.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5490..5696)
                     /locus_tag="PWS53_001131"
     CDS             complement(5490..5696)
                     /locus_tag="PWS53_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871390.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5791..7293)
                     /locus_tag="PWS53_001132"
     CDS             complement(5791..7293)
                     /locus_tag="PWS53_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL0871391.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7311..7766)
                     /gene="argR"
                     /locus_tag="PWS53_001133"
     CDS             complement(7311..7766)
                     /gene="argR"
                     /locus_tag="PWS53_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0871392.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7864..8481)
                     /locus_tag="PWS53_001134"
     CDS             complement(7864..8481)
                     /locus_tag="PWS53_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL0871393.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8702..10075
                     /locus_tag="PWS53_001135"
     CDS             8702..10075
                     /locus_tag="PWS53_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871394.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10150..11262
                     /locus_tag="PWS53_001136"
     CDS             10150..11262
                     /locus_tag="PWS53_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL0871395.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11334..12971)
                     /locus_tag="PWS53_001137"
     CDS             complement(11334..12971)
                     /locus_tag="PWS53_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL0871396.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13139..13747)
                     /locus_tag="PWS53_001138"
     CDS             complement(13139..13747)
                     /locus_tag="PWS53_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0871397.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14131..15282)
                     /gene="queG"
                     /locus_tag="PWS53_001139"
     CDS             complement(14131..15282)
                     /gene="queG"
                     /locus_tag="PWS53_001139"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL0871398.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15301..16053)
                     /locus_tag="PWS53_001140"
     CDS             complement(15301..16053)
                     /locus_tag="PWS53_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871399.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16194..17144)
                     /locus_tag="PWS53_001141"
     CDS             complement(16194..17144)
                     /locus_tag="PWS53_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0871400.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17145..17984)
                     /locus_tag="PWS53_001142"
     CDS             complement(17145..17984)
                     /locus_tag="PWS53_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0871401.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17981..18727)
                     /locus_tag="PWS53_001143"
     CDS             complement(17981..18727)
                     /locus_tag="PWS53_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0871402.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18965..20335
                     /locus_tag="PWS53_001144"
     CDS             18965..20335
                     /locus_tag="PWS53_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0871403.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20390..21286)
                     /locus_tag="PWS53_001145"
     CDS             complement(20390..21286)
                     /locus_tag="PWS53_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0871404.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21400..21570)
                     /locus_tag="PWS53_001146"
     CDS             complement(21400..21570)
                     /locus_tag="PWS53_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871405.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21567..22244)
                     /locus_tag="PWS53_001147"
     CDS             complement(21567..22244)
                     /locus_tag="PWS53_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0871406.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22335..23240)
                     /locus_tag="PWS53_001148"
     CDS             complement(22335..23240)
                     /locus_tag="PWS53_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0871407.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23415..24869
                     /locus_tag="PWS53_001149"
     CDS             23415..24869
                     /locus_tag="PWS53_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871408.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24885..25916
                     /locus_tag="PWS53_001150"
     CDS             24885..25916
                     /locus_tag="PWS53_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0871409.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25923..27110
                     /locus_tag="PWS53_001151"
     CDS             25923..27110
                     /locus_tag="PWS53_001151"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL0871410.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27266..28648
                     /locus_tag="PWS53_001152"
     CDS             27266..28648
                     /locus_tag="PWS53_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0871411.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28620..29006
                     /locus_tag="PWS53_001153"
     CDS             28620..29006
                     /locus_tag="PWS53_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871412.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29206..29874)
                     /locus_tag="PWS53_001154"
     CDS             complement(29206..29874)
                     /locus_tag="PWS53_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL0871413.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8446 a         6158 c         5501 g         9994 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagaaacta
      121 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
      181 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
      241 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
      301 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
      361 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
      421 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
      481 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
      541 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
      601 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
      661 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
      721 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
      781 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
      841 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
      901 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
      961 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     1021 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcataaaa gccactcatt
     1081 gggaaaacac aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc
     1141 tctgcacggg cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg
     1201 atttccggag tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga
     1261 aaaacttctc cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga
     1321 taataattct gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc
     1381 cctcccaatc tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt
     1441 tcaatttttg tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt
     1501 ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt
     1561 gaaattgttc cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg
     1621 ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtaga aagccatttt
     1681 cacaaaattt ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg
     1741 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta
     1801 tttttatttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta
     1861 aacggtgttc aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
     1921 tattttgccg tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata
     1981 cacggtgttc cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga
     2041 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta
     2101 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
     2161 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
     2221 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
     2281 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
     2341 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
     2401 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
     2461 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
     2521 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
     2581 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
     2641 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
     2701 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
     2761 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
     2821 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
     2881 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
     2941 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
     3001 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
     3061 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     3121 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     3181 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     3241 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     3301 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     3361 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     3421 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     3481 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     3541 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     3601 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     3661 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     3721 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     3781 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     3841 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     3901 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     3961 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     4021 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     4081 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     4141 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     4201 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     4261 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     4321 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     4381 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     4441 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     4501 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     4561 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     4621 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     4681 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     4741 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     4801 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     4861 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     4921 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     4981 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     5041 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     5101 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     5161 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     5221 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     5281 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     5341 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     5401 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     5461 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     5521 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     5581 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     5641 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     5701 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     5761 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     5821 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     5881 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     5941 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     6001 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     6061 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     6121 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     6181 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     6241 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     6301 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     6361 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     6421 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     6481 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     6541 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     6601 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     6661 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     6721 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     6781 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     6841 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     6901 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     6961 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     7021 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     7081 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     7141 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     7201 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     7261 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     7321 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     7381 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     7441 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     7501 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     7561 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     7621 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     7681 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     7741 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     7801 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     7861 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     7921 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     7981 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     8041 taagatttgg gcacaaagac cttcttccat ctttttgaat actagttgtt ctttcaagtc
     8101 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     8161 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     8221 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     8281 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     8341 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     8401 tggtttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     8461 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     8521 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     8581 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     8641 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     8701 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     8761 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     8821 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     8881 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     8941 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     9001 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     9061 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     9121 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     9181 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     9241 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     9301 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     9361 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     9421 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     9481 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     9541 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     9601 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     9661 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     9721 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     9781 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     9841 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     9901 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     9961 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
    10021 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
    10081 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
    10141 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gactctagcc
    10201 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
    10261 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
    10321 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
    10381 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
    10441 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
    10501 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
    10561 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
    10621 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
    10681 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
    10741 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
    10801 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
    10861 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
    10921 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
    10981 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
    11041 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
    11101 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
    11161 tatatcggct atctattagc caacttctta aatactggtc attggtctac ggaagattat
    11221 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
    11281 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
    11341 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
    11401 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
    11461 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
    11521 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
    11581 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
    11641 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
    11701 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
    11761 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
    11821 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
    11881 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
    11941 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
    12001 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
    12061 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    12121 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    12181 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    12241 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    12301 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    12361 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    12421 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    12481 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    12541 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    12601 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    12661 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    12721 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    12781 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    12841 gctgtcttcc tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    12901 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    12961 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    13021 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    13081 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    13141 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    13201 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    13261 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    13321 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    13381 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    13441 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    13501 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    13561 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    13621 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    13681 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    13741 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    13801 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    13861 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    13921 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    13981 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    14041 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    14101 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    14161 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    14221 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    14281 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    14341 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    14401 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    14461 ctttaatttt ggatatactt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    14521 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    14581 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    14641 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    14701 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    14761 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    14821 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    14881 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    14941 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    15001 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    15061 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    15121 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    15181 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    15241 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    15301 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    15361 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    15421 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    15481 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    15541 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    15601 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    15661 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    15721 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    15781 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    15841 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    15901 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    15961 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    16021 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    16081 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    16141 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    16201 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    16261 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    16321 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    16381 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    16441 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    16501 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    16561 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    16621 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    16681 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    16741 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    16801 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    16861 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    16921 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    16981 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    17041 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    17101 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    17161 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    17221 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    17281 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    17341 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    17401 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    17461 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    17521 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    17581 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    17641 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    17701 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    17761 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    17821 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    17881 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    17941 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    18001 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    18061 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    18121 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    18181 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    18241 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    18301 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    18361 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    18421 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    18481 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccggctgt ccagaaaagg
    18541 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    18601 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    18661 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    18721 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    18781 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    18841 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    18901 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    18961 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    19021 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    19081 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    19141 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    19201 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    19261 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    19321 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    19381 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    19441 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    19501 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    19561 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    19621 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    19681 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    19741 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    19801 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    19861 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    19921 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    19981 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    20041 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    20101 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    20161 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    20221 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    20281 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    20341 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    20401 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    20461 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    20521 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    20581 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    20641 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    20701 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    20761 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    20821 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    20881 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    20941 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    21001 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    21061 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    21121 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    21181 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    21241 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    21301 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    21361 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    21421 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    21481 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    21541 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    21601 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    21661 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    21721 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    21781 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    21841 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    21901 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    21961 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    22021 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    22081 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    22141 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    22201 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    22261 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    22321 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    22381 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    22441 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    22501 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    22561 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    22621 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    22681 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    22741 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    22801 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    22861 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    22921 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    22981 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    23041 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    23101 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    23161 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    23221 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    23281 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    23341 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    23401 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    23461 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    23521 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    23581 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    23641 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    23701 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    23761 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    23821 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    23881 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    23941 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    24001 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    24061 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    24121 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    24181 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    24241 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    24301 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    24361 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    24421 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    24481 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    24541 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    24601 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    24661 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    24721 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    24781 cacaaatcgt tgaggaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    24841 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    24901 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    24961 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    25021 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    25081 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    25141 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    25201 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    25261 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    25321 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    25381 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    25441 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    25501 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    25561 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    25621 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    25681 gaatccaagt aggtattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    25741 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    25801 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    25861 ctggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    25921 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    25981 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    26041 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    26101 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    26161 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    26221 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagttg ttagtggaag
    26281 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    26341 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    26401 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    26461 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    26521 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    26581 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    26641 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    26701 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    26761 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    26821 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    26881 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    26941 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    27001 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    27061 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    27121 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    27181 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    27241 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    27301 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    27361 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    27421 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    27481 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    27541 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    27601 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    27661 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    27721 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    27781 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    27841 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    27901 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    27961 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    28021 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    28081 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    28141 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    28201 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    28261 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    28321 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    28381 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    28441 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    28501 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    28561 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    28621 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    28681 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    28741 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    28801 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    28861 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    28921 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    28981 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    29041 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    29101 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    29161 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    29221 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    29281 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    29341 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    29401 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccagc
    29461 attctctagg ataacatgtt tggcattttc aagagaaaag cattcttgct gtcttaagat
    29521 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    29581 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    29641 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    29701 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    29761 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    29821 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    29881 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    29941 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    30001 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    30061 ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DANJJH010000028        30009 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000028 DANJJH010000000
VERSION     DANJJH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(240..1967)
                     /locus_tag="PWS53_001155"
     CDS             complement(240..1967)
                     /locus_tag="PWS53_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL0871414.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1960..3153)
                     /locus_tag="PWS53_001156"
     CDS             complement(1960..3153)
                     /locus_tag="PWS53_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0871415.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3340..3984)
                     /locus_tag="PWS53_001157"
     CDS             complement(3340..3984)
                     /locus_tag="PWS53_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0871416.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4078..4212)
                     /locus_tag="PWS53_001158"
     CDS             complement(4078..4212)
                     /locus_tag="PWS53_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL0871417.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="PWS53_001159"
     CDS             complement(4214..6346)
                     /gene="feoB"
                     /locus_tag="PWS53_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL0871418.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6343..6816)
                     /locus_tag="PWS53_001160"
     CDS             complement(6343..6816)
                     /locus_tag="PWS53_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL0871419.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7217..7441
                     /gene="nrdH"
                     /locus_tag="PWS53_001161"
     CDS             7217..7441
                     /gene="nrdH"
                     /locus_tag="PWS53_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL0871420.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7600..9759
                     /gene="nrdE"
                     /locus_tag="PWS53_001162"
     CDS             7600..9759
                     /gene="nrdE"
                     /locus_tag="PWS53_001162"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL0871421.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9809..10774
                     /gene="nrdF"
                     /locus_tag="PWS53_001163"
     CDS             9809..10774
                     /gene="nrdF"
                     /locus_tag="PWS53_001163"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL0871422.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10863..12002)
                     /locus_tag="PWS53_001164"
     CDS             complement(10863..12002)
                     /locus_tag="PWS53_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0871423.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12311..13024)
                     /locus_tag="PWS53_001165"
     CDS             complement(12311..13024)
                     /locus_tag="PWS53_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL0871424.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="PWS53_001166"
     CDS             complement(13278..13979)
                     /gene="nagB"
                     /locus_tag="PWS53_001166"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL0871425.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14213..15745
                     /locus_tag="PWS53_001167"
     CDS             14213..15745
                     /locus_tag="PWS53_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0871426.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15936..17138)
                     /locus_tag="PWS53_001168"
     CDS             complement(15936..17138)
                     /locus_tag="PWS53_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871427.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17220..17909)
                     /locus_tag="PWS53_001169"
     CDS             complement(17220..17909)
                     /locus_tag="PWS53_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0871428.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18015..18383)
                     /gene="rplL"
                     /locus_tag="PWS53_001170"
     CDS             complement(18015..18383)
                     /gene="rplL"
                     /locus_tag="PWS53_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL0871429.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18436..18939)
                     /locus_tag="PWS53_001171"
     CDS             complement(18436..18939)
                     /locus_tag="PWS53_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL0871430.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19143..19270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19349..20038)
                     /gene="rplA"
                     /locus_tag="PWS53_001172"
     CDS             complement(19349..20038)
                     /gene="rplA"
                     /locus_tag="PWS53_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL0871431.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20142..20564)
                     /gene="rplK"
                     /locus_tag="PWS53_001173"
     CDS             complement(20142..20564)
                     /gene="rplK"
                     /locus_tag="PWS53_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL0871432.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20907..21575
                     /gene="sdaAB"
                     /locus_tag="PWS53_001174"
     CDS             20907..21575
                     /gene="sdaAB"
                     /locus_tag="PWS53_001174"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL0871433.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21588..22460
                     /gene="sdaAA"
                     /locus_tag="PWS53_001175"
     CDS             21588..22460
                     /gene="sdaAA"
                     /locus_tag="PWS53_001175"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL0871434.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22551..23570)
                     /locus_tag="PWS53_001176"
     CDS             complement(22551..23570)
                     /locus_tag="PWS53_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0871435.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(23743..24648)
                     /locus_tag="PWS53_001177"
     CDS             complement(23743..24648)
                     /locus_tag="PWS53_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0871436.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(24707..25252)
                     /gene="nusG"
                     /locus_tag="PWS53_001178"
     CDS             complement(24707..25252)
                     /gene="nusG"
                     /locus_tag="PWS53_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL0871437.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25353..25523)
                     /gene="secE"
                     /locus_tag="PWS53_001179"
     CDS             complement(25353..25523)
                     /gene="secE"
                     /locus_tag="PWS53_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL0871438.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25547..25699)
                     /gene="rpmG"
                     /locus_tag="PWS53_001180"
     CDS             complement(25547..25699)
                     /gene="rpmG"
                     /locus_tag="PWS53_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0871439.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25861..26505
                     /locus_tag="PWS53_001181"
     CDS             25861..26505
                     /locus_tag="PWS53_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0871440.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26561..27064)
                     /locus_tag="PWS53_001182"
     CDS             complement(26561..27064)
                     /locus_tag="PWS53_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0871441.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27077..27123)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27661..28626
                     /locus_tag="PWS53_001183"
     CDS             27661..28626
                     /locus_tag="PWS53_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0871442.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28725..29897)
                     /locus_tag="PWS53_001184"
     CDS             complement(28725..29897)
                     /locus_tag="PWS53_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871443.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29884..>30009)
                     /locus_tag="PWS53_001185"
     CDS             complement(29884..>30009)
                     /locus_tag="PWS53_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871444.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9137 a         6178 c         5358 g         9336 t
ORIGIN      
        1 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
       61 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
      121 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
      181 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
      241 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
      301 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
      361 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
      421 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
      481 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
      541 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
      601 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
      661 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
      721 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
      781 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
      841 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
      901 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
      961 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
     1021 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
     1081 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
     1141 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
     1201 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
     1261 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
     1321 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
     1381 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
     1441 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
     1501 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
     1561 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
     1621 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
     1681 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
     1741 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
     1801 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
     1861 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
     1921 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
     1981 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
     2041 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
     2101 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
     2161 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
     2221 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
     2281 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
     2341 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
     2401 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
     2461 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
     2521 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
     2581 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
     2641 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
     2701 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
     2761 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
     2821 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
     2881 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
     2941 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
     3001 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
     3061 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
     3121 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
     3181 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
     3241 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
     3301 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
     3361 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
     3421 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
     3481 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
     3541 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
     3601 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
     3661 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
     3721 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
     3781 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
     3841 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
     3901 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
     3961 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
     4021 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
     4081 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
     4141 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
     4201 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
     4261 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
     4321 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
     4381 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
     4441 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
     4501 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
     4561 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
     4621 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
     4681 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
     4741 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
     4801 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
     4861 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
     4921 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
     4981 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
     5041 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
     5101 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
     5161 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
     5221 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
     5281 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
     5341 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
     5401 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
     5461 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
     5521 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
     5581 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
     5641 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
     5701 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
     5761 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
     5821 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
     5881 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
     5941 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
     6001 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
     6061 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
     6121 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
     6181 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
     6241 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
     6301 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
     6361 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
     6421 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
     6481 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
     6541 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
     6601 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
     6661 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
     6721 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
     6781 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
     6841 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
     6901 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
     6961 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
     7021 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
     7081 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
     7141 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
     7201 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
     7261 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
     7321 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
     7381 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
     7441 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
     7501 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
     7561 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
     7621 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
     7681 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
     7741 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
     7801 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
     7861 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
     7921 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
     7981 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
     8041 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
     8101 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
     8161 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
     8221 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
     8281 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
     8341 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
     8401 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
     8461 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
     8521 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
     8581 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
     8641 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
     8701 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
     8761 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
     8821 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
     8881 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
     8941 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
     9001 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
     9061 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
     9121 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
     9181 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
     9241 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
     9301 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
     9361 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
     9421 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
     9481 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
     9541 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
     9601 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
     9661 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
     9721 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
     9781 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
     9841 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
     9901 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
     9961 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    10021 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    10081 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    10141 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    10201 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    10261 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    10321 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    10381 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    10441 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    10501 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    10561 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    10621 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    10681 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    10741 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    10801 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    10861 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    10921 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    10981 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    11041 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    11101 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    11161 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    11221 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    11281 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    11341 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    11401 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    11461 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    11521 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    11581 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    11641 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    11701 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    11761 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    11821 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    11881 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    11941 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    12001 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    12061 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    12121 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    12181 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    12241 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    12301 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    12361 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    12421 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    12481 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    12541 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    12601 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    12661 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    12721 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    12781 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    12841 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    12901 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    12961 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    13021 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    13081 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    13141 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    13201 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    13261 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    13321 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    13381 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    13441 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    13501 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    13561 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    13621 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    13681 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    13741 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    13801 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    13861 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    13921 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    13981 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    14041 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    14101 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    14161 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    14221 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    14281 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    14341 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    14401 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    14461 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    14521 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    14581 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    14641 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    14701 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    14761 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    14821 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    14881 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    14941 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    15001 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    15061 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    15121 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    15181 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    15241 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    15301 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    15361 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    15421 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    15481 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    15541 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    15601 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    15661 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    15721 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    15781 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    15841 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    15901 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    15961 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    16021 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    16081 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    16141 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    16201 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    16261 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    16321 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    16381 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    16441 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    16501 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    16561 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    16621 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    16681 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    16741 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    16801 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    16861 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    16921 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    16981 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    17041 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    17101 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    17161 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    17221 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    17281 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    17341 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    17401 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    17461 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    17521 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    17581 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    17641 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    17701 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    17761 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    17821 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    17881 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    17941 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    18001 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    18061 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    18121 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    18181 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    18241 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    18301 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    18361 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    18421 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    18481 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    18541 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    18601 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    18661 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    18721 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    18781 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    18841 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    18901 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    18961 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    19021 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    19081 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    19141 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    19201 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    19261 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    19321 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    19381 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    19441 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    19501 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    19561 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    19621 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    19681 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    19741 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    19801 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    19861 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    19921 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    19981 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    20041 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    20101 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    20161 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    20221 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    20281 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    20341 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    20401 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    20461 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    20521 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    20581 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    20641 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    20701 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    20761 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    20821 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    20881 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    20941 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    21001 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    21061 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    21121 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    21181 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    21241 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    21301 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    21361 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    21421 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    21481 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    21541 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    21601 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    21661 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    21721 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    21781 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    21841 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    21901 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    21961 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    22021 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    22081 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    22141 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    22201 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    22261 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    22321 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    22381 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    22441 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    22501 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    22561 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    22621 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    22681 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    22741 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    22801 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    22861 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    22921 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    22981 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    23041 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    23101 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    23161 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    23221 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    23281 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    23341 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    23401 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    23461 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    23521 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    23581 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    23641 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    23701 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    23761 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    23821 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    23881 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    23941 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    24001 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    24061 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    24121 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    24181 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    24241 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    24301 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    24361 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    24421 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    24481 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    24541 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    24601 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    24661 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    24721 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    24781 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    24841 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    24901 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    24961 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    25021 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    25081 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    25141 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    25201 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    25261 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    25321 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    25381 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    25441 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    25501 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    25561 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    25621 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    25681 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    25741 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    25801 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    25861 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    25921 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    25981 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    26041 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    26101 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    26161 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    26221 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    26281 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    26341 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    26401 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    26461 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    26521 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    26581 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    26641 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    26701 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    26761 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    26821 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    26881 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    26941 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    27001 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    27061 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    27121 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    27181 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    27241 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    27301 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    27361 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    27421 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    27481 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    27541 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    27601 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    27661 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    27721 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    27781 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    27841 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    27901 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    27961 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    28021 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    28081 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    28141 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    28201 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    28261 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    28321 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    28381 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    28441 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    28501 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    28561 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    28621 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    28681 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    28741 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    28801 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    28861 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    28921 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    28981 aagcaactgc ctgtgataaa aatatctttg atcccgccgc tggtttctga gttttttacc
    29041 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    29101 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    29161 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    29221 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    29281 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    29341 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    29401 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    29461 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    29521 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    29581 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    29641 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    29701 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    29761 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    29821 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    29881 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    29941 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    30001 ataaagttt
//
LOCUS       DANJJH010000029        29433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000029 DANJJH010000000
VERSION     DANJJH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(37..1368)
                     /locus_tag="PWS53_001186"
     CDS             complement(37..1368)
                     /locus_tag="PWS53_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0871445.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1517..2230)
                     /locus_tag="PWS53_001187"
     CDS             complement(1517..2230)
                     /locus_tag="PWS53_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0871446.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2405..3829
                     /locus_tag="PWS53_001188"
     CDS             2405..3829
                     /locus_tag="PWS53_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0871447.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4128..4835
                     /locus_tag="PWS53_001189"
     CDS             4128..4835
                     /locus_tag="PWS53_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0871448.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4822..5145
                     /locus_tag="PWS53_001190"
     CDS             4822..5145
                     /locus_tag="PWS53_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0871449.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5234..6271)
                     /locus_tag="PWS53_001191"
     CDS             complement(5234..6271)
                     /locus_tag="PWS53_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0871450.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6592..7575)
                     /locus_tag="PWS53_001192"
     CDS             complement(6592..7575)
                     /locus_tag="PWS53_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL0871451.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7990..8760)
                     /locus_tag="PWS53_001193"
     CDS             complement(7990..8760)
                     /locus_tag="PWS53_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871452.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8753..9688)
                     /locus_tag="PWS53_001194"
     CDS             complement(8753..9688)
                     /locus_tag="PWS53_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871453.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10004..11653)
                     /locus_tag="PWS53_001195"
     CDS             complement(10004..11653)
                     /locus_tag="PWS53_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0871454.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11770..14373)
                     /locus_tag="PWS53_001196"
     CDS             complement(11770..14373)
                     /locus_tag="PWS53_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL0871455.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14385..15434)
                     /locus_tag="PWS53_001197"
     CDS             complement(14385..15434)
                     /locus_tag="PWS53_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL0871456.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15460..15774)
                     /locus_tag="PWS53_001198"
     CDS             complement(15460..15774)
                     /locus_tag="PWS53_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL0871457.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15790..16236)
                     /locus_tag="PWS53_001199"
     CDS             complement(15790..16236)
                     /locus_tag="PWS53_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871458.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16237..18159)
                     /locus_tag="PWS53_001200"
     CDS             complement(16237..18159)
                     /locus_tag="PWS53_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL0871459.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(18371..19267)
                     /locus_tag="PWS53_001201"
     CDS             complement(18371..19267)
                     /locus_tag="PWS53_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0871460.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19260..19940)
                     /locus_tag="PWS53_001202"
     CDS             complement(19260..19940)
                     /locus_tag="PWS53_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871461.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(19941..20723)
                     /locus_tag="PWS53_001203"
     CDS             complement(19941..20723)
                     /locus_tag="PWS53_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871462.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20716..21633)
                     /locus_tag="PWS53_001204"
     CDS             complement(20716..21633)
                     /locus_tag="PWS53_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871463.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21797..22120)
                     /locus_tag="PWS53_001205"
     CDS             complement(21797..22120)
                     /locus_tag="PWS53_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871464.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            22908..26135
                     /gene="fss3"
                     /locus_tag="PWS53_001206"
     CDS             22908..26135
                     /gene="fss3"
                     /locus_tag="PWS53_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL0871465.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26248..26562
                     /locus_tag="PWS53_001207"
     CDS             26248..26562
                     /locus_tag="PWS53_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0871466.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            26575..26949
                     /locus_tag="PWS53_001208"
     CDS             26575..26949
                     /locus_tag="PWS53_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0871467.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            26959..27294
                     /locus_tag="PWS53_001209"
     CDS             26959..27294
                     /locus_tag="PWS53_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL0871468.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            27377..28717
                     /locus_tag="PWS53_001210"
     CDS             27377..28717
                     /locus_tag="PWS53_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0871469.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            28795..>29433
                     /locus_tag="PWS53_001211"
     CDS             28795..>29433
                     /locus_tag="PWS53_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL0871470.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGY"
BASE COUNT         9062 a         5758 c         5220 g         9393 t
ORIGIN      
        1 gacttcttgt gttaattttt gttatctcat agtaaattat gtttcatttt cacttaattt
       61 agttgtcacg tgttcgtcaa ttcgtaaaaa cggtaaatat ataaacacac ttgcggctat
      121 aattaatatt tgtaaaacag ctgctcgcca gtctccggct gttgctaaaa atcccgatat
      181 aactggtggc gtagtccaag gaatttgcac tactacatga ttaattaatc ccacagcagt
      241 tgcaaaatat gctactgtca aagaaaaaat cggtgcaagc acaaagggaa taatcagact
      301 gatattaaat acaataggca atccaaaaat aataggttcg cttatattga atatagcggg
      361 agttaccgat aatttagcaa agtccttata atcttttcta cgactgaaga taaatattgc
      421 aatcaataaa gcaatcgtat ttcccgatcc acccattacc gcaaaggtat ctttaaaggc
      481 catattgata atatgtggaa tttgctcttt gtttgcataa gctgccatat tttcttgcat
      541 attctgaatc aaaaacggat ctaacaatga acctgatatt actgcttgat ggataccaaa
      601 tccaaaaaat agattagcaa tagatgtaat taacaaaaat cctggtaaac cagttgtaat
      661 tttacttaat ggctctgtaa ctatcgtggt aattagctta tttatatcca tattaaacaa
      721 ttgaatgatt ataaatgaca ataaagcaaa cgaggtaatc gtaataatga ttgggattaa
      781 tacattaaaa gactttaaaa cagcgggagg aatatttccg gatagctgaa tctgtagttt
      841 cttattatta gaaagtttaa taaataattc tgtagccgct aatccaacaa atatacctac
      901 aaacattcct gaagctcctg tataggatat aggtatcaca cccgtcactt caacagcttt
      961 atccgcattt tcaggagtaa acatcatctg cataggtgtt aatacaacaa atgtcgaaat
     1021 agcaactaat accgctgcaa tatgatttgt ataatttctg cttgcacaca tatgataaga
     1081 tactgctgct acaactaata atgtagttat gcttaatgta ccattggcaa ttgaagtacc
     1141 aatttgttgt aacgtggtca atgtactagt atcgacaatt tcccccatga atcctgttgg
     1201 ttctaagatc actgaattaa taagtgtcac aaatcctgct aacatcataa atggcataaa
     1261 tgtagcaaac gcatctcgca aagttcttaa atggatctga ttccccaact tagtcgcaac
     1321 tataccaaat ttttcaaata caatgtcctt cctgctactt ttgcccatat tttttctcct
     1381 ttatgcttat tgtaatcgat tactttttcc acccaaataa tacatcactt atcaaaaaaa
     1441 gtaaatactt ttaattaaat atatatatct tttttagtat aaaaaaataa gctctgaaaa
     1501 aataattttc ccgagcttat ttttcaacaa aaacagcttc aaaaacaaag tcttgatata
     1561 aatgtctcga taccgaatac tcgaaaggtt taccagacga tagagcaaca actttgtcta
     1621 tctcaatcat aaattcacct ggatctattt ttaatatttt tgcgtcaaat tcattcgcag
     1681 tatctccttt gatacgtatg tgcgaagtct ttatttccag acccagttca tttgtgatat
     1741 aagcatatac agatttcttt aaatgttttg gttccaagcc tggtatgact gctaatggca
     1801 tgaatgtttg ctcaatcgag tatggttgac aatcaataaa acggcttcgt acaatattat
     1861 atacatagtc ttttacactg atacttaatt tatcagctac ttttttcgat ggaagttcaa
     1921 ttgagaaagt atggatttct gaatctacgt gttcttcacc aaccacgcta aacaaacctt
     1981 cgtttggacc aagaactcgt gagttatcaa ttggtgttct gacgacttca gtcccatacc
     2041 cacttctact tcgcaaaact ccttcggaaa caagtaagtc tacgcctttc tttactgtta
     2101 acttgcttgt atgataaata actgccaatt catctcctgt aggaagtttc tcaccaattt
     2161 tatactctcc atttacaatt tttttcttta tatctaagta aatttccata tacttcgcaa
     2221 tttttttcaa tggtgaccct ccaatcaaaa acatatcttc atctatactt taacataaat
     2281 aaaccgataa aatccacatc aaaattatac acacaataaa aaagatatat atatttaaac
     2341 aaaactattt actttaagtt gtaagcggtg ttatcattaa gtggaaacag gaggtaaaaa
     2401 aattatgaca actatgaaaa agctaacttt tccagatgat ttttggtggg gttccgcttg
     2461 gtcagctgag caagcagaag gaaaaggaga aacaggaaaa gcagagacta tctgggatcg
     2521 ctggtttaaa gaacaacctt accggtttta tcaacgaatc gattcctcta ttacaaccga
     2581 tcacatccat caatataaag aagatataga actaatgaaa aaaactgggc ataactcctt
     2641 tcgcgtttct atttcttggg cacgaatgtt tccaaatggc ggtaccggaa aagtaaatcc
     2701 caaagccatt tctttctatc gaaaattatt ttccgaaatg aataataacg gaatcaaagt
     2761 atttgccaac ttatatcatt ttgacatgcc tgctactctt caagataaag gaggatggga
     2821 atcacgtgaa gtagttgatg cttatgttca ttacgctgaa acttgtttta aagaatttgg
     2881 tgacttagtt tgtcattggt ttaccttcaa tgaaccactt gggcctattt taggtactta
     2941 tcttgaagat tttcattatc caaacctcat tgactttaaa cgcggagcgc aagcagcatt
     3001 cttcactata cttgcacacg caaaagcaat acaagtattt aaagaactag ctcttcctag
     3061 taaaataggc gttattctaa atttgagtcc tacttttcct cgtagtcaaa atcctgctga
     3121 tgtgaaagca gcagaaatcg ctgatttatt ttatacgcgg agctttttag atcctatggt
     3181 taaagggaat ttttctaaaa agcttatcct gttattacaa gaatataatc agcttcctga
     3241 agattatacg aaagaagatc ttgtatgtat ctacgaaaat accgctcaaa ttcttggact
     3301 aaactattac gagccacgtc gcgtcaaatc tcgttcttct atggtggata catctagtcc
     3361 atttttacca gagtggtttt tcgaaccaca tatcatgcct ggacgacgaa tgaacgaata
     3421 ccggggatgg gaaatttacg aaaaaggaat ttatgatttg tgcatggata tcaagaacca
     3481 ttatggaaac atcgaagcat ttatctcgga gaacggaatg ggcgttgcgg atgaagaacg
     3541 tttcatggat gatacagggc aaataattga tgattatcgg attgagtata tcaaagatca
     3601 tcttgcttac ttatggaagg caataaatga aggatgtaac attaaggggt atcatctttg
     3661 gacatttatt gattgctggt catggatcaa cgcctataaa aaccgatatg ggctggtttc
     3721 actcgacctt actactcaaa aaagaacaat taaaaaaagt ggcgaatttt acaagcaatt
     3781 aagtgataca aatggattta gatacgatgt agacttgtta gttgaataat attctaaaat
     3841 tccacttgaa gtgaacacat tagaaaacaa gaataatgag aaataccaat aacaaatact
     3901 ttttcttcat acttcattta tacttgtcag tataagagac tgtgacaaat gtagtgatcc
     3961 tcagaagtta ggtctggaaa tctaacttga gaagattttt ttaaactacg tccagtcttt
     4021 tttatttgtt taaaattgaa gttgacaagt cttcattaaa aatttagaat attcacaagt
     4081 ttgacgacaa ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt
     4141 tagatataaa aactgcgatc aaagacacgt tacctaccgt ttttggttat atcggtattg
     4201 gacttgcatt tggtatcgtt gggaaagctg ccgggtttca tccgctggtc gtcacgttga
     4261 tgtccctgct gatctatgct ggttctgccc aatttatcac ggtcagcatg cttgctagcc
     4321 atagtccact gctctccatc gttctctcga cctttctagt caattcccga atgattctga
     4381 tgagtatgac gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg
     4441 gcacattgct gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac
     4501 aaaagttaaa ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg
     4561 gttcttctct tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt
     4621 tggatttcgc catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata
     4681 gaaacacctc taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg
     4741 tcggactgat ttttatccca agtaacttag tgattttagt tgtaacactg attgggtgcg
     4801 gattgggggt gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt
     4861 tgtactttag caacttggct ttctaggatc ctaccatttg tcttactgaa aaaattcgat
     4921 ttgccgcaac cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta
     4981 tggttcagca gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat
     5041 gctttggctt cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc
     5101 gtcttggtag gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag
     5161 cttatggcaa attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc
     5221 ggcgtattct tgcttatccg ttgaacggac cactaacaac gacttttcct aatatatgtc
     5281 ctgtttctac gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga
     5341 ccttgctcaa tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag
     5401 cttctccttg cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt
     5461 cgaagctgat agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga
     5521 tcgttcccac atgtccaaat ggtcggacaa ggtgtttgat ttgatcaaag tagcgggtaa
     5581 tatcgaataa tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac
     5641 ttaagtcgtg atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat
     5701 tcttcggact tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa
     5761 tcgaaccgac gcccccagct ccgttgatca ctaagatttt tttcccgcgg ttggcatttt
     5821 cttctggagt cagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg
     5881 gcaaggctgc ggcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt
     5941 caaccagttg gtattcttga ttgcttcctg ctcttgtcgt tgtacctgcg taaaaaacgc
     6001 gatcgccgac agaaaatttt tgcacttcat cacctacagc aacaactttc ccgactccat
     6061 caaaccctaa gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga
     6121 caggattaac agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta
     6181 tctgttcttt ttcatcaata aagctttttg gatcatcgat cgacaaccct tcataaaaac
     6241 cgactacctt cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg
     6301 gagcatccat acagttttca tttcaatata tatagtatgc tatttgttcc acttttattc
     6361 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     6421 tgcgacaaga cttttgtcac aacctctttt aaacggtgtt caatcaactg accctcggta
     6481 ttcgctcaaa aaacgacaaa atatgaggag ctatttttgt ttggctgttc ttattacttg
     6541 atgcaggaca gctttttcac gacctctaat tcacggtgtt caagcaagaa atcattgttc
     6601 actgaaaagc gttggatgtt ctttttcaaa ggtgtctaag atctctcgta cttcatcagt
     6661 agatttcgta ttcatcaact gaactcgcaa gtcatttgct cctggaaatc ctttgatata
     6721 gattttaaag aaacgatgta gtcctgtgat ggaacgcggg atgatttctg aataatgatc
     6781 ttgcagatct agttgcagac gaagtagtcc gatcagttct ttttctgtat gttccctcgg
     6841 ttctttttca aatgcataag gattcttgaa aatcccccgg ccaatcatga tgccatctac
     6901 gccatatttt tcggccaatt cttctcctgt ctggcgatct gggatatctc cgttgatcgt
     6961 gatcagtgtt tgcggagcga tttggtcacg taaagcaatg attttaggga tgacttccca
     7021 atgtgcatct actttactca tctctttacg cgtacgcaaa tggacagaaa gattggctat
     7081 atcttgatga agtaggtgac tgatccattc ttccatctca gacatttccg tatagccgat
     7141 acgcgtcttg acgctgacag gcaaaccgcc agctttagcc gcttcaatca attgtgccgc
     7201 tacttctggg cgaaggatca agccactgcc ttttccacgt tctgctacat tagggactgg
     7261 gcagcccatg ttgatatcga ttccttggaa gcccatttct gccatagcaa tgctcatttc
     7321 tctaaaaaat tctggcttgt ctccccaaat atgggcaacc atcggctgtt catcttctgt
     7381 aaaggtcaat cttcctcgta cactgtcttt tccatctgga tgacagaaac tgtctgagtt
     7441 tgtaaactca gtaaagaaga catccggtgc tgctgcatgc tttacgacat ggcgaaatac
     7501 aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa ggctttggca gctctttcca
     7561 aaaattagta gtcatttatt tgttactctc cattctatca agcagtgaag tcgcaatctt
     7621 tctcaaaaac attgtcatta tactaggaaa caaaaaaaga agcaaagtga acagcttaaa
     7681 agatgtttct gattgagcaa aaaaagactg tgacaaaaat cttatcacaa ttccttttcc
     7741 ggaataaatg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa acgaaaaata
     7801 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     7861 ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca aacgacaaaa
     7921 tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
     7981 acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag taacggcgta
     8041 caaaacaacg atgaatagca caagtacgat catcgatgtt gctactggca catcgattgt
     8101 tccaaggaat ccgtaacgga aaccggagat catataaacg atcggattga ctttcgaaac
     8161 agcttgccaa aatggcggca acatagagat ggcatagaaa acgccaccta gataagttaa
     8221 cggctgcaag acaaatgtag gcacgatcga tacatcgtca taagactgag caaaaatccc
     8281 gttgagcagt cctgctaacg aaaacaagat tgccgtcatc aataatgtaa tgatgaccat
     8341 gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta cgagagagcc
     8401 gacgagaata ctgcgaccca ctccgccaat cacaaagccc caaatgatca catgcgtagg
     8461 aacaggcgct accaagattt cttcgatgtt tttctggaat ttttgagaga agaaggacga
     8521 agaaacgttg gcatacgaac tagtgatagc agacatcata atcaaaccag gcacgatgaa
     8581 ttccatatat gaaaaaccac ccatgtcacc gatccgacca ccgatcattt ttccgaaaat
     8641 aacaaagtac aaggatgtcg tgatcacagg cggaaccagt gtttggaccc agatacgcaa
     8701 atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa gattaaacat
     8761 ttttctcctc cgtatgatta ttttcttctg tgattttcaa aaacagttct tctaaacgat
     8821 tcgacttatt cctcatagaa agaactttga ttccttgtct tgtcagctga tcgaagattc
     8881 cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgttcatct tcaaatgtac
     8941 aagcgtatcc ttcaatcaca ggtttggtct catatggcgc taaatcaaag ataaatgttt
     9001 caaattgcag tttggataga agctctttca tactagtatt ctcgatcagt tcacctgatt
     9061 ggatgatccc gatgttgcga caaagcattt cagcttcttc taaataatgc gtcgtcaaaa
     9121 tgatcgttgt accattttcg ttcaattctc ggagaaactc ccacatctcg cgtctcaatt
     9181 caatatcgac tcctgctgtt ggttcatcca agatcagcaa acgtggttca tgcatcaatg
     9241 ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca cggacatttc
     9301 tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc gcttctttgc
     9361 gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt tcaaagggat
     9421 tgaaattgaa ctcttgcggg actaatccaa tctgttgctt tgctcggact agatccgtat
     9481 ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa gaagtaatga
     9541 ttccgatcgt tgtagacttc ccagctccat ttggtcctaa tagcgcatag aaatctcctt
     9601 ctttcacgga aagatcgatt cctcgcaatg cttcgacccc acttccataa acctttttca
     9661 aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga catgattctt
     9721 tattgtatca tggaatacag cagatgaaaa aacaacttgg ttccagcgaa aaatcaggtt
     9781 gctgggctca ggaaaatctc ctaatgaaag acatcaatcg gacgaagtca gacaaccttt
     9841 tcagaaaaaa caaatcgaag ataaaggatt actattcata tgtgcaacaa aaggagcgtg
     9901 agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcaa
     9961 aagccaagat ttttgatcca ataaatactt tcgatcactt cgcctatccc aatacatgcc
    10021 actgccgctt ttcatcaaat aacgcggctt ccgtttttgc ggtttctact aaagtctctg
    10081 ggtaatccaa aactttcagc aaggcaatcg catttcgggt ccttgaaggt ccttctttta
    10141 atttgaaatc aaaactgatt ccttttcctt ctgttacttg ttcttcaaag tggacattct
    10201 cacaataatt ctttaaaatc tctgtcaatt cgatatcatg tgtggcgaca aatgctaaag
    10261 atggatactc cgcaagccac cgaacgactg aagcggaaga agcaatccgt tcgatcgtat
    10321 ttgtcccttt taaaatctca tctacgaagc agtagcagcg ttctttggaa gccacttggt
    10381 ccaacaaacg tttgatcgat ttgatttctg aaacaaaata actgtcgcct tcaaataaat
    10441 catcttcgat tgccatggaa gtcaacacat gtccaggttg cagggtaaac tgttctgcaa
    10501 tcgctgtttg gatcgtctgt gctagaatac agctgatggc tatacttttg acatacgtag
    10561 attttcctga tgcattggaa cccgtgacta atgtattctt ctgccagttg accggattca
    10621 ctaccgcttc ttttaacaat ggatgataga tatctttcgc tgtcacaccg ccctcagtaa
    10681 actctggtcg gcaagtgatg ggcatataag tacggtaatt caatattgca gcggctactt
    10741 ctaattctcc cagcaattcc cacagcttga tggcttcagt tgaatgcttt tctaatttag
    10801 tgagaacgaa atgataggag ataaatggca gcatgaacat gatgttgaca tattcaaaaa
    10861 tcaactctgc ttcactccca ctcttcatac gaaatgaaaa acctaattta gggatgtctc
    10921 ttaatggttt agtttgctgt ttcagctctt cttgaagtgg tgtcggcagt tttgctagct
    10981 gattggcggc tgaaatcgtt gaaaccaaat aactcatgct attcagctct gtttctagtg
    11041 tttgtttctt cactagataa tagaccgtat tgaatactaa cccaccgatt gccaaataga
    11101 ttcctagtac ttgtcctaaa agaagcaaac ccaaacccac cagcggaagc aagccaagaa
    11161 aaataaataa acctacgcgt ccaatctttt tactttttcc atccgctaga tattgttttg
    11221 caaaattatg atcttttttt cctaaacgag cgaattgata ttggattttt tctctcagtt
    11281 gaggattttc ctgataaaaa gcaatcagtt tttctaactg ctgatcttca ccaaaatcaa
    11341 agctgcgtaa acgttgatac aacgcttctg aacctacact agaataggta ctgttgattc
    11401 cttcgaatac ttcgaacata tccaggtcat accaagtaag atcatcgatt tcgctatccc
    11461 atgaacggaa cttcttctcc gtgagccatg cttccttcaa gctttcttct ttatcaaatc
    11521 tcggttgata aggaattttt tcccatttgc tacgaactgt ttcctttaat ttcaaacgtg
    11581 aaaaaatgtc taaagcaact aataaaataa cagctgcaat aataattaaa acgataaatt
    11641 ctctttgacc catatccctc tccgttcctt tatttgttag ctttagtata gcactgttgc
    11701 gctatttgaa aagagggtaa aaaaacacct ttcaaaataa aaggtgtttt tttcttgtat
    11761 attacatatt tatctctcat ttatcaaaat tgacaaataa tcatagggag gaattgttgt
    11821 taattcttgt ttattctcca gtgcattgac aactgtccca agggaaagga tagcctcagg
    11881 tactattttt tcttgttctg ttggattagc taagcgaaga acaaatcctt gttcatgaaa
    11941 ataagaagga taaatcgatg atacaattaa gtcttcaggt aattcaatca atgaaaattt
    12001 acgaggaaca tcaagttcat tttgtaatgg ccaaatcttg ttatctaacc tgtgaataaa
    12061 tacattcagt ttctgctttt gataagaaat agattgttct aaccgttttt ctgcctgttt
    12121 tgccactttg aactcattaa atcttccttc ttctacatta aatccaaaag tgactttata
    12181 ctcacctatc tcttgagcta gcggtgtttc catcatgaca tgcccttcat ttgtcgtatc
    12241 tcctgaagct cgcccaggtc gccaagcaag attaggtttt ccaagttccc ctgttgttga
    12301 aaataaggtt atgattaatt gattttctat acgttcatac tcttttaaac catctgcata
    12361 aaacgtaaaa taatgatcat tttctgccac agagacactc ttatcgaata tttctaaatt
    12421 aacaggtttc tctaccatag tttctgacca attcggagga atgacatcag gggtattttc
    12481 aataaatccg ttttgaattt gtgcaattga tttttcagat ttatctaaaa cattaattgc
    12541 taagcgcata cgatgcgagt aaacagtatt atgaattttg agtgtgcctt caacgatttg
    12601 actttctccc agactgaacg atatttcata agatatttct tccatttcgg gtttttcaga
    12661 taaacgatct tttaaatctt taggaagttg agcttttccg taaacaacta atgtttcttg
    12721 taaagaatct ttatatactt tagctgtttc taattttaga gatagttctg tatctccttc
    12781 taatggtgaa taatcatacg tatcgccatc atttgctgaa tcgatcaaat gaacgaaatc
    12841 aagatattgt ctatctccaa ctatcaaatt caattgccca tctttaaaag aaagtttata
    12901 tttattgtta cttatctcag ttttgtctat ctcgattgct gtttctaatt tttcattatt
    12961 tttctcaaat tcaacaacag tgtatcctag tgctggcaca tttacattta tttttatgtc
    13021 aagttcataa taagctggtt catcaaaata ttcgaaacct gtagcaatca tccgataagc
    13081 atttttccgt tctggaaaat aaacttcttt ttcaatgata ttatagggac tacctttgaa
    13141 gtgtattttc tttgaatcag ctactacatg cacgattttc gatccactat aattctcagt
    13201 cgttgtgttg aaaacaagga cttcattcga ttttagcttt aatgcatcag ccatccgttt
    13261 tacaataagg ttttcgattc cgtcagctat ctcattagct tctttgattc gatggaaaat
    13321 atcttccgca acattatctg aaacacagcc accaatacta tcatgagcct ggttttgcaa
    13381 aatctttttc cataaccgac gaagtaggcc tttactgata tggataccat ttctttctgc
    13441 aatcagcatg agcggctcaa ctcgttttag aattttttgt tccaatttaa aattttctat
    13501 tttcattttt gttcttactg agccaataga acgatgaaca cgagagtaaa ccggctgacg
    13561 aagttcacct ttatattcag gtaattcttt tttgtttctg ataacttcta cataagaagg
    13621 ataatctgaa ataatattgt ggaatttact gtatttattc aacttctcta cgacttgatc
    13681 aaaatttaaa atcattgact tttgatctac acccgaaggt actaaaatat ctttgttttt
    13741 tccttgcata tgaagcattt caactgcttc atccaacttt tcttttgcaa attcaggaat
    13801 atctttaatt gcgtctaagg tcatcattcc agtcatataa ccaaatggaa aattcatggc
    13861 ataaacaaat ttatctccca atcctttcca gataaaataa ggagatggaa ccatcgtttc
    13921 aaagtttaat cctcgccaac acatgaaatt atcgatttct acttgatgca aaagtgtagg
    13981 caaattcgca ttgaacccaa acgtgtcagg caaataccct accatcattg gatctccata
    14041 tttaaggatc gtatcactga ttccaatcat taagtttctt aaaattgatt cagcatccac
    14101 taataaggca tctgtttgtg cataccacgg tcccacgaat agttgtcctg ttttcatcag
    14161 tcgttggata accttaagct tttgcggatt gatctccaaa tactcatcga cgattgaagt
    14221 ttgtccatct aaacaaaagt ttactttagg gttcctttcc aattcatcta gaacttcagt
    14281 aaagacctga tcagacaata cgatggcatc ttcatttgta aaataccatt ctctgtccca
    14341 gtgcgtgtgt tgaattatat gtgcatttac catatcagat tctcctactt tccttcttct
    14401 actgaattta cttgttgttc ttttttatac ttgatggatt ttagtgcatt gaccattaac
    14461 gctgtaacca ctcccccaat aacaattgat actaaatata aaggtatatt cgtaacaaaa
    14521 ggaattgcca taaaggttga aagcattgtt tcttgagtaa caccaaaaaa tccatttaga
    14581 ccacatgcta ctgcagtgcc caccacgatg cttggtatta ctcgtaaagg atctttcacg
    14641 gcaaacggga tcgtcccttc agtaatcccc acaaggccca taatcatagc tgtctttgca
    14701 ttatctcgtt ccgattcatc ataaagtttt ttgtttaaaa ttgttgctaa aaacattcca
    14761 aatggcggaa tagcaattcc taataacatg gctgtactag gtccaaagct tccgcttgaa
    14821 aaagaagcaa ccccaaaagc atatgcgatt ttattgatag gtcctcccat atcaaacgcc
    14881 atcattcctc ctaaaatagc cgacatcaaa gcagcatttg ctcctgacat gccttctagc
    14941 caattagtca gtcctgttgt cattgcttga ataggcgtag ctagtacata ccataatatg
    15001 attccaacaa ctaatgtagt aacaaaagga atcagcataa ttgattttag actaataact
    15061 tgcatcggta cttttacttt tttcaatgca ttagctaaat agccacaagc taatccagca
    15121 accattcctc caataaatcc cataccaaga ttttgacaca tcatcccagt gataaaagct
    15181 ggtgctagac cgggacggtc agcaattgaa tatgcgatat aggctgaaat catcggtacg
    15241 ataaactgta atcccgtctg accaatcatt cgcaggactc cccagaatgt atccgtctgg
    15301 tcccaaacac ctgtgccacc aaaaatgact gcaatagaag ttagtaaccc tgctgctacg
    15361 acaagcggga tcatatagga aaccccactt tggaaatgat ttagaatttc tcttccttgt
    15421 ttcttgaatt ccattagtaa ctcccccacg atatattttt tatttgttaa ttaatgcaat
    15481 tgcttcatca atcgtctctt caccatcttt tactacctct gaggtcccta ctgttaaaat
    15541 cggtttaccg tcaaagcgac ctcgttctga aacatttgta tctactgcaa atatgacaac
    15601 atctgcctcg gaaacatccg aaattgaaag tttgttttct atccccatag ccccctgtgt
    15661 ttctacttta atatctaacc ctttcttctt cgcaaacttt tctaaattag cttttgccat
    15721 ataagtatgg gcaatccctg cgatacatgc tgttactgct actatttttg tcatatgaaa
    15781 ttcctccttt taaaatgata gagcttttac caattctttt ttatttctcg aatcttttat
    15841 tcttttaaca aactcgtcat ccatcaatct tccagataat tctgctaaaa tacgtaaatg
    15901 attagtacct ttatcagtgt cttttattgc taacaaaaag acgagatcaa ctggttgatc
    15961 atctaatgcg ccccattcaa cagaccgctt taacttggct acagccactg ttgattcaat
    16021 tactgctagg cttttgccat gaggaatggc gatatttttc ccaattcctg tggtcatatg
    16081 gttttctctg tcaaatacgt ctttttgaaa ttgtttgtgg tcagacacaa acccattagt
    16141 ctctaacaat ttcgataatt tttcgactac acttttttta tcagatgcat ttaaatctat
    16201 cactatatta tttatattga ttttttcact gatcatttat aagccttctt tcaatatata
    16261 aactaatttt tcaaacgacg aactcgtttt taaatttgct aataactcat gattcgaagc
    16321 gatttcattg aatgtttcat aaattttatt caattcaggt tttacttttt cattaagtgc
    16381 aaaaaagaaa acagaatgaa caagtgtccc atcccaatca attccttctg gattaacgta
    16441 gacacagatt actggctttt ttataaaatc tacttcgata tgcggcatag caaaatcatt
    16501 catcgctgtt gaagatagtt tttctctttt gattgctccc tctagtatcc cttctttcgc
    16561 gtaattttct tttaccaatt ttttggtaat tatatttaaa acgttcactt gattatcctt
    16621 tatttggtgg gtaaaaaagc aaaatcgttt atctaataaa tttagaaaag catcttctgt
    16681 ttgttcattt aactgtagat actgttttac ttttctgaca tctgccccta acatcaaagg
    16741 actaacaaca ataacaggaa aatttgtatt ttcaatcgga atcgtgctta taacaagttc
    16801 atcagtgact tttctatctt gtagttcatt gatgcttagt acatcttcta tctcaaccat
    16861 atctgcaaaa ccgttattta ttctttgctc taataatttt gatgttccat atccgctact
    16921 acaaactaat atcactttcg ttttgtcagg tttgtaacga tccaaaagtg attgaatatg
    16981 gagtgttacg taagcgattt catcttcatt aaaatgtata cagaatctct cttcgatttc
    17041 ctctaataaa tctacactta tcataaatga ccttttaaaa gaatatttga ttttgtccgt
    17101 ataagggtta tagatattta ctcccaattt gagtctttta actgctgcat tgagatgtaa
    17161 tactagatta ttgatcaatt cttcatcagg ataaaattca tttaatttaa ttcttactaa
    17221 ctcttgtagc tcaacaccca tatctaaata ggaaagcttc gtttgatcta agtcgtgatt
    17281 catagcattt ttttcaatcg aagtaatgtg aatgtcaaca taagcttgtt catctattgg
    17341 cacaataatt gaaaatgctt tttcaatcat tttgattaaa ataagtgtat tctttgagat
    17401 ttcttcttca gaattattac tacgtgataa gaaattgctt gtttcaatcc tttctatcgc
    17461 aatagcaagg tggatagcta aagtttgaaa ttcataatct gtaaatttca attgtgtctt
    17521 attcatgtat cgattaagca attcggctac ttttttcaat atgtccttat ttaagaaatt
    17581 attcgcttca ccaatgaaat tgatattcaa gtctaagaga tctgttttac tgttagatgc
    17641 tgtaaagcca ccccaataat agttcacaac ttctgacatc aaccttcttt tacttttttc
    17701 atcagctttg atgcgcacgc catttcgatc tgaaactaag gacatcccat gcttgtttag
    17761 tctttttcta acatctttca ttgtgctttc taccgtcgac ctgctcacgt ataattcgtc
    17821 agcaagattt tgaatcttta gagaatcatt agtattcaat aatttagcaa acacataagt
    17881 tagacggtcc tccggagagt ctgcgatatt gattttatga ctggataata aatttcgaag
    17941 tgtttctttt tttccaataa gcgaaacgcc ttttttaggt tgaaccaaca tttgtacatc
    18001 tggcattttt tggattattt ctttcaaaat taaaatatct ttgctaattg ttttcgtaga
    18061 tacatataat tgttctgata attctttgta agttgtgtgc tcattttcaa tcaaaaagta
    18121 tagtattttc atttgccgtt cgctaaattc gatgcccatt atcttcacct cctgaataca
    18181 gcttaagtgt acaattatta tctctatttt catacagaag aaagttcttg attaatgtag
    18241 aagtttctaa tattcttgat tatttcaaat caataaaaaa gcctgcaata tttatatcac
    18301 aggctttttt ctctattaaa tatatataag ataatacatt tttctactag ttctcactaa
    18361 tcttgtataa ttaaaccctc cgcaacttga cggagatgga gaagatgccc ccgtctaatc
    18421 tagcggtcat ctctcctccc attccttcca ccaatttttt tgacaggtaa agcccgattc
    18481 cgctagattc cgtatttgtg cggctggtat tttcggtata aaaacgctgg aagacttttt
    18541 caacagaggt ttgcgttcct ggtttgatag gattggccac tgttaatcga atgaatggat
    18601 cctcttttga aaatgtcacc tttgcttgtc catctgcata acgcaataaa ttctgtatga
    18661 tattttgcag cactcgcagc aacatgagcg gatctgctaa tacagttgta tttatcgagt
    18721 gttcagcgaa cgctacctcg atatttttct cagaaaattg ttcataaaaa gagaaaaata
    18781 cttcttccac ctctttagaa aaagaaagcg tatggagatc catttctttt tgcttcgtct
    18841 ctattcgagc aagatcataa aactgatccg tcatactgag taatcttgca acagattgtt
    18901 tgatgatcgt taaatacttc tcccgtttct ccgtatcctc tacctgttcc agtaattgta
    18961 cataaccgtt aatagcagtc aaaggagttc tgagatcatg agaaaggccg acgatcgata
    19021 gtctgatgtt ttcttccatt tcttgaaatg cttgtccttt ttgttcattt tcatccaacc
    19081 atgtattcag tgcatctact aggtccatta gattcttttc tcgaaattcg actgaaagac
    19141 gaccaccata ttcttgtcgt tcagaaagag tggcgatttt cttagttaat ctcttgattt
    19201 ccctttgcag gaagaaaata aaacttccca taaagataac aacgagaagc aacagccatt
    19261 taatcaaatc taaaccccac tccccacacc gtatgaatat acacagcatg tgcctttgct
    19321 agcttccctc gaagattgga aatatgcata ttcactgttt tctcactatc aaaatatggt
    19381 tcttgccaaa ctgtttcata aagatttgct cgcgaaaata cttttttcgg atgttctaaa
    19441 aaaagcttca gcagatcgaa ttctcttcct gtcaaatgga cagccacacc attcacattt
    19501 acatccctcg actctttgtc cagtaagatt tccttgtaat agatgcgttg cgacagatct
    19561 tttacttgga cagaagtctg atgacgaagt tggatgccga ttcgtaacaa aagttcttcg
    19621 atatcaaatg gtttcgtcac atagtcatcc gctcctgctg ccaacaagga aactttatct
    19681 ctttgatcgc tttttgctga aagaacgatg atcggaacat tcgatgtctg tcggatttct
    19741 gctaacaatg cttcacctga aagccctgga agcatgagat ctagaaggat caaatcgaca
    19801 ggatatgtgc ttaacaataa ttttccttcc gtacctgaat aagcatcaag aagtttgtat
    19861 ctctgttcaa gaacctcctc cagtaaggaa tgaacagctt catcgtcttc gataatcaaa
    19921 atagtttcca gcaggaccac ctacttaatg tcagattttt ggaaaataaa cattcctaga
    19981 gctgatgata ccacgattgt tactagactg accagaatca ttttcggtgc ttgcgaataa
    20041 gttgcttgtt gcgcaaagtc cgtatacatg agccacgttt ccgtaaacca ctccgttaga
    20101 aattccaagc ctttgatttt tgcgaaaaca ccgggaatga aggccgttcc catgatataa
    20161 agaaaagaaa gaagcaaact gacactggta cttctagtga tgaatgtcaa agccactgga
    20221 acagataatg tccctaatgt cagcagaagc aatacaggaa atacttttag aacataattt
    20281 ccatagtttt tgattccctc aacttcacca aatccgaaag caagtgtgta agcaagcatg
    20341 gctagtcccg tgatcaaaac cacgccaagt gttgtgatca aaagcgtaac gagaagtttc
    20401 gacacataat aagtcattcg attccgtcct aaagataacg tattgcgaat cgtaccgttt
    20461 tgaaattctt ccccaatata aaagcacgca aatgctgcga aagccagaag aatccatgca
    20521 gccatttttt ctaccattaa aatccctgtt gcaccgttga tgactgtatc cgttgcttgg
    20581 tttaattcat tgccgtattt tgtatgcata taccattcgt tccaagaata aaaaagcgga
    20641 agtacgacca tcagtcctag tgagacccac attttacggc tcttccatac ttttattttt
    20701 tcagcagtta atagattaaa catgtggatt gcctccagtt aattgtaaga aataatcttc
    20761 cagtttttgc ccaactaggt gaataccatc cacaggaact tttgcttcta ccaatgcttg
    20821 attgatggct gctacttggt caattccttc aaaaagctgg ataacacggc tagattgtac
    20881 aaaataatcc ctataaccaa gagagtcgag aaccgttact gcttgttccg tcgccgaagt
    20941 gtcaattttt ataaattgac gagattcttg tgccaattct tctttcgata attctttgat
    21001 caagtgtcct tcatgcaaaa taccatagtg agtggcgatc tgtgacaatt catctagcaa
    21061 gtggctagaa agcaagacgg tgatgccccg ttcttttacc aaacgttgga tgatttctcg
    21121 catttctgcc atccctgctg gatccatccc attcgtcggt tcatccaaaa tcaaaaattc
    21181 tggattcgtg atcagtgcat ttgcaatagc taaacgctgg cgcataccta aagagaaatt
    21241 ctttgctttc tttttccctg tattttccaa tcgcatcatt ttcagcaggt cttcgatttc
    21301 tctatccgac acaccgccgt tcgctgcttg gatacgtagg ttatctcttg cagttaattc
    21361 tggataaaga gcaggggttt caatcgattg acccatacgt cgtcttgctg cctgcaaatc
    21421 cgtcgttcca aataattcga tcgaaccttc atcaatcgag atcaaaccca taatagttct
    21481 cataaaggta gacttacccg ctccattttc cccaatcaat ccatagatca ttccacgttt
    21541 gatttcgatt gatactttat ctaatgcttt ggcagcaccg tattttttgg ttataccagt
    21601 tgctttcaag accgtttcat ttccaaaatc catacttatc cttctttctt attgatgagt
    21661 gaagtatagt tgacgggtat aaagaaagca gccagaaatg tataaagaaa gtgtaaattt
    21721 tccaattgaa tgtcaaaaaa aacaatcgga caatacgtgt ctttcctatt atcaggcagt
    21781 ttgttatacg cttgtatcag tttcctgatt ctttcttttc gaccaaagtc gtttctctgt
    21841 attcctcttc actgaaaatt tttgcttctt tcagatcaag cttgagtaat tttaccagac
    21901 cggttataag gataactata gcaagactcc ctcccagaaa aattagactg tctcgcagac
    21961 gtggttgcgt tgtcttccaa tcaaagagcg caccaaacag tgctaatcca agtggactgg
    22021 ccaccatggt aatcgttgtt atcgttgaca taagtcgtcc ttgtatatgc gttggaactt
    22081 ctcgctgaag ccagaccata taaaaagcaa aagaaaacat taatccttaa aaacggactt
    22141 atgaaaaata atacatgccg tttgggattt acaattagct accaaataag aggttatgac
    22201 aaaagacttt tcacaatccc ttcttccgaa taaacggtgt tctcctgcca actcctcggt
    22261 cacaagtcac aatatttatc aaacatgaga agcaattttt gtaaattcct tcttatttct
    22321 tgaagctgac cacttctgtc acaacctcag aagcggataa ataatattgt ttgatgatac
    22381 tttatctttt tcccgtttgt ttatagatcg aatgatatta tagaccaaga atcctaccta
    22441 ttaacatagc taaaaacaca aaaaaggcac tttccaaatt gtggaaagtg ccagaaaagt
    22501 taggattaag gtaactgcgc tcagcattgc ttcgcccagt acgattctcg taactcgtga
    22561 actcgaacga taatcgcaat taacgttttg agaactgtga agctttacgg gctttcttaa
    22621 gacctggttt tttcttcgta tcattgaata agtttgaaaa accttgattt ataaaggttt
    22681 tcccgtaaat atattttatt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg
    22741 ttctaattta aaggcagtat atttcgtatt ttagattaaa tttttaggct cattcattct
    22801 caaatccatt ctattcagtc aataaaaatt ttaaaaagac tttataaaca aagatgttca
    22861 agcgagcatc ttattttttt gtgcttaatt tgaaaggaga acatttcatg tataaaaaac
    22921 aaaaaaagaa attatgtgta ttaactattt tgagtttggc tgttcaagca attgttggag
    22981 caattgctcc tacgattact tttgctgatg aaattaccca tccacaaacg gtaaccgtag
    23041 aactagattt agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt
    23101 cagaagtcac tgttccgcat tatgcagtgt acaacggctt taaacaagat attttctgta
    23161 ttgaaccagg agtcccgatt tacaatgagt tcactcctgg ctatgagaaa aacccgttac
    23221 cagatatgtc cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg
    23281 cagatactca tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc
    23341 actcaataaa acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaataa
    23401 atcaagccat tgacgattat cagaagaaac caaccttcca taatagtacg gctaaaacag
    23461 tattagggca atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg
    23521 aggttgtgga aaatgccgca aacattgatt atcgtgtgaa tggtaatcag ttgataatca
    23581 ccccaaatgc caattcaaaa gaaagtggtg tgctgacgct taagaaatct gctggtaccg
    23641 gaacacctgt cgcctataaa aaggctggcc aacagacttt aatggccggg gcgattgata
    23701 aacctaatac ctacacaatc aaaattgatg tggaaacgga gggttcttta aagatcaaaa
    23761 aagtcgataa agaatcgggt gatgttgtac caggaacggt tttccattta gactttggaa
    23821 aaactttacc tgcaaaagac gtgactactg acaaagaagg cattgctaca ttggatgaga
    23881 ttcctcatgg tacaaaagta accattaccg aaaaatcagt accggcgcct tatacgattg
    23941 ataccacccc catgactacc accattaaag caggcgaaac catttacgtg acttcaaaaa
    24001 acgcacggga aaaagggcaa atcattctgg ataaaagcgg agttgaaaca gggagtgatc
    24061 tttggaatga caattattcc ttagctggta atacgtttgc cattcgcaaa gacagtccaa
    24121 ccggtgaaat cgttcaagaa atgaccacgg atgaaaacgg tcatgcggaa acaccaaaag
    24181 agattgctaa tgcgttggaa ttgggaacct attatgtgac cgaaactaaa gctagtcatg
    24241 ggttcgtgaa tacgttcaaa cctgtaaaag tcgaattgaa gtatgccaat caaaccgtag
    24301 cccctgttac cagtaacgta aaagggcaaa atcaagaagt cactggcgaa accactttga
    24361 cgaaagaaga caaagatact ggggataaag cacaaggtaa ggcagtcttt gagggtgctg
    24421 aatacacctt gtttactgct aaggatggaa aagctgttaa atggagtgag gcttttaaac
    24481 cagagttagc gaagggaacg aaaggttctg atgaaacggt cactttgact ttagacgaaa
    24541 agaaccaagt tgccgttaaa catttagcta tcaacgagta ttattggcaa gaaacaaaag
    24601 cgcccgaagg atatagcctt gatgaaacaa agtatcctgt atccatcaaa aaggtggatg
    24661 atagcgaaaa aaatgccgtg attacccgaa atgttacggc aaaagaacaa gtgattcgct
    24721 ttggctttga tttctttaaa tttgctggat cagctgcggg cactaccgaa actggcttca
    24781 atgatctgac ctttaaagtg tcgccattgg aagggaccaa tgaaatcaca ggtgccgaag
    24841 atgaagcgac cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa
    24901 atcttcctta tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga
    24961 ttacgccatt agaaatccgt tctactttta aggaaaacaa agaagacttt gtgaagagtg
    25021 agtatgtctt taccattacc gaacaagacc aaaaacaacc aattaaaacg gtgaccgttc
    25081 cttacgagaa gctgacgaac aaagcatttt ctgttagttt gaaccgttta atgctctatg
    25141 atttgcctga ggaagaagat agtttaactt ctcttgcgac ttggaaagac ggaaacaaag
    25201 aattaacttc tctggattct accgagctag ttgataaatt gagttacaat ctacatgaaa
    25261 tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggatgcgaca aaagctgccc
    25321 aagaaaaaga tgaaaaagcc aaaccagtgg tgattgcaga aacaagcgca acgttggcga
    25381 acaaagagaa aacaggaact tggaaaattc agcataaatt aaccgccgaa caagttttaa
    25441 ataaaactat tgtcttattc aactatgtgt atgaaaataa ggaagctttt gaagcaggcg
    25501 ataagccagt agcgaaggat gttagcttga ataaccaagc ccaaactgtc agttgtacag
    25561 tggaacacca tgtttctatc caaacaaagg cccacctaga aaacggttct cagaccttta
    25621 ctcatggtga tgtggtggat atgtttgatg atgtgtcgat tactcatgat gtactggatg
    25681 gctcaaaaga agctttcgaa acaattctgt atgctttatt accggatggt acaaacaaag
    25741 aaatttggaa atctggcaag attgattatg aagtaaatga taaagaattc accaaaactg
    25801 tgcttgcgaa aaaagtagat accggaaaat atccagaagg aaccaagttt acttttgcgg
    25861 aaatcaatta cgataaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa
    25921 aatctcaaac cttaacacca aaagaagttc caaccatact aagtacgccg aaacaaccgg
    25981 aaacaccaac tgttccaagt ggctctcaag aatctagtcc cacagtgaag accttccctc
    26041 aaaccgggga gaaaaattcc aatgttttac tgttcattgg ttttactttg atttttgcga
    26101 cggcgggcta ttatttctgg aatcgccgga actaaggtga tgagatgata caagtaaatc
    26161 ctaagaacca acgtgcgcga ccgcctccta aaaaagtagt cgcgctattt ttgtatccaa
    26221 aactaacaat gaaaagagga aaagaagatg gaattgaaat ttgtcgtgcc agatatggct
    26281 gaaacctttg gaaagttaag ttatgccggt gaaggagaaa ttataacaga aggatatgga
    26341 cggaatacta cggtgattgg tcgcagttat catttgtatt ccagtaagca acgggccgat
    26401 gatattgagg tggtagtagc tgcggaagcc ggtgaaaagg attttgatca agaccaaccg
    26461 ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt
    26521 actgattacg tgttgtatgt tgatgattta gtgaaagcat aggaggaaaa gaagatgaga
    26581 ttaacagaag gtattgtcgt agattcaggt ttaacatttg ggaagttgcg tttttccgca
    26641 ttacgccgag aagtacggaa gcaaaatgag gatggaacaa ttggtaatga ggtaaaagaa
    26701 cgaacctata atttaaaatc ctctgcacaa gggcgaatga ttcaagttag tatcccagct
    26761 aatgtaccct tacgcgagtt tgcttatgat gcagaggtag agttggtcaa tccaatcgtg
    26821 gataccgttg ccaattatgt ttttcgagaa ggaactaccg ttaattggtt cattaaggcg
    26881 gatgatttag tcttgaagcg gcagccgaat caaggaaatc ctacgaacca aaatgaaaga
    26941 aagaaatagg tgaagattat ggaaattaat gttgaacata ttttagattg tctcgatcaa
    27001 tatggaaaag gcgagctgac tgaggaacaa ctcgttaaag cattaacctc tgatgaaaaa
    27061 atgtttctga ttatacatca aggtttactt ggagaaaata gcgacagtga agaagactct
    27121 gatgtactag actggttagg agaagaagag tctttcttta tagttgtcga agtaaatgaa
    27181 aacttatgtc ggcaggcaga atccattttg gaagaaattg gtgtggagat gccggatgct
    27241 attgaaggct ttttaaatca attggttgaa acgaaacaac tcccctcctc gtagcagtaa
    27301 atgactagca taactttttg aaattgccta atgaaatcct attgggcttt ttcgattgga
    27361 ggtggaaacc atcaaaatgt acaaaggaca tcgcatacgc gcaggagatc agcacttggt
    27421 ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta
    27481 ttttcaagag tttagacaat ttgatatttc taagctatca ctttctacta ttgaaatcgt
    27541 ttggtctata aaagatttag tatgtctgct tggaagtctc gctttttcag gagctatgat
    27601 attgctttat attcactttt ttcttgatca ttggcgaagc ttgtggcatc gacaaaagct
    27661 agctcggatg attctagaga accattggta tgaagtgaaa caaactcaaa gtgaaggctt
    27721 tttcaaagat ttgaatagta gccgaaccag agagactatc agttacttcc ccaaaatctg
    27781 ttatcgaatg aaagatggat tactgtctat tcgcgttcaa atttcacttg ggaaatacca
    27841 agatcagctt ttaaaattgg aaaagaagtt agaaagcgga ctgtattgtg agttagtaga
    27901 aaaggaactc aaagactcct atgtggagta tactttactg tatgatatga tcgccaatcg
    27961 aattgggata gacgaagtag tggcagaaaa tgggactctg cgattgatga aaaatcaagt
    28021 atgggcttat gattctttac cccatatgtt aatcgctggt ggcacgggtg gagggaagac
    28081 ctatttcctc ctcaccatta tcgaagcact attgaagtca gatgcagaat tgtttatcct
    28141 cgatcccaaa aatgcagatt tagctgattt aggtacggtg atgcctcatg tttattcaca
    28201 aaaggaagaa atttctgctt gtgtggaaga tttctatgaa cgtatgatgg ctcgtagcaa
    28261 ggcaatgaaa gaaatgtcta actacaaaac aggagagaat tatgcctatc ttggacttcc
    28321 acccaacttt ttaatctttg atgaatacgt ggcttatatg gagatgttaa cgacgaaaga
    28381 aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttgggtcgtc aatctggttt
    28441 ctttctgatt ttggcttgcc aaaggccgga tgcgaaatat ttaggagacg gaatccgcga
    28501 tcaattcaac tttcgcgtgg ctttgggtcg tatgagtgaa ctcggatatt ccatgatgtt
    28561 tggggaggtc gaaaaaaact tctttatgaa gcacatcaaa ggtcgaggtt atgtggatac
    28621 aggtggcagt gtgattagtg agttttatac gccacttgta ccaaaagggt atgatttctt
    28681 agattcaatt agtaaattag aaaagatgaa tgattgagtg atcttcaata ctgactttat
    28741 acttcaagta acgtataata cttttatata gtaggaagaa aggggataat taatatggaa
    28801 agtaatgatt cttatttaga gtatgcaaaa gcaaataagg ataagtttat ttcttatatt
    28861 atcaaaggga aatcccccga agttggacga gacgcagttt ttatggcagg gagtcctggg
    28921 gctggaaaat cagaggttgc cattggatta gctgagaatt atgataacca tgtgattatt
    28981 gatgcggatt actttcgcac ccagtttcct gattataatg gaaaaaactc tagtctcttt
    29041 caaaaagcat cttcttggtt ggtagagcag gcatttagat atgtcttaga tcatggctat
    29101 tcttttattc tggatgcaac ttttgcaata ttaagtgctg aaaaaaatat ctcgcgggcg
    29161 ttgaaaaaac attttcgagt gacgattttc tatgtttatc aagaccctaa aatcgcttgg
    29221 aaatttacac aagaacgtga aattgcagag ggacgtcatg tgccgaaaaa ggcatttatt
    29281 aacgcttttt tcaaatcaag agagaacatt gcaaaagtaa agagtaaaca tccagatgta
    29341 ttgcttcata tcataataaa agattatacg aataatattt ccgaagttca ttatgctacg
    29401 gataatctag aattattttt accactaggg tat
//
LOCUS       DANJJH010000030        27853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000030 DANJJH010000000
VERSION     DANJJH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="PWS53_001212"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="PWS53_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL0871471.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="PWS53_001213"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="PWS53_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL0871472.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="PWS53_001214"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="PWS53_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL0871473.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="PWS53_001215"
     CDS             1642..2274
                     /locus_tag="PWS53_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0871474.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="PWS53_001216"
     CDS             complement(2276..3181)
                     /locus_tag="PWS53_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0871475.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="PWS53_001217"
     CDS             complement(3266..3925)
                     /locus_tag="PWS53_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0871476.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="PWS53_001218"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="PWS53_001218"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL0871477.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="PWS53_001219"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="PWS53_001219"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL0871478.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="PWS53_001220"
     CDS             6168..7253
                     /locus_tag="PWS53_001220"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL0871479.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="PWS53_001221"
     CDS             7250..8314
                     /locus_tag="PWS53_001221"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL0871480.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="PWS53_001222"
     CDS             complement(8563..9366)
                     /locus_tag="PWS53_001222"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL0871481.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="PWS53_001223"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="PWS53_001223"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL0871482.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="PWS53_001224"
     CDS             complement(10244..11251)
                     /locus_tag="PWS53_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0871483.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="PWS53_001225"
     CDS             complement(11258..11902)
                     /locus_tag="PWS53_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL0871484.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="PWS53_001226"
     CDS             complement(12002..12760)
                     /locus_tag="PWS53_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL0871485.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="PWS53_001227"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="PWS53_001227"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL0871486.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="PWS53_001228"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="PWS53_001228"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL0871487.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="PWS53_001229"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="PWS53_001229"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL0871488.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="PWS53_001230"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="PWS53_001230"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL0871489.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="PWS53_001231"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="PWS53_001231"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL0871490.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="PWS53_001232"
     CDS             18158..19027
                     /locus_tag="PWS53_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL0871491.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="PWS53_001233"
     CDS             complement(19153..20052)
                     /locus_tag="PWS53_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871492.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="PWS53_001234"
     CDS             complement(20217..21041)
                     /locus_tag="PWS53_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0871493.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="PWS53_001235"
     CDS             complement(21052..22347)
                     /locus_tag="PWS53_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL0871494.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="PWS53_001236"
     CDS             complement(22363..22842)
                     /locus_tag="PWS53_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL0871495.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="PWS53_001237"
     CDS             complement(22848..23876)
                     /locus_tag="PWS53_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL0871496.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="PWS53_001238"
     CDS             complement(23893..24999)
                     /locus_tag="PWS53_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0871497.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="PWS53_001239"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="PWS53_001239"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL0871498.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7851 a         6122 c         5034 g         8846 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tat
//
LOCUS       DANJJH010000031        27726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000031 DANJJH010000000
VERSION     DANJJH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(290..1234)
                     /locus_tag="PWS53_001240"
     CDS             complement(290..1234)
                     /locus_tag="PWS53_001240"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0871499.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1530..2474)
                     /locus_tag="PWS53_001241"
     CDS             complement(1530..2474)
                     /locus_tag="PWS53_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871500.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2695..3909
                     /locus_tag="PWS53_001242"
     CDS             2695..3909
                     /locus_tag="PWS53_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871501.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3931..5946
                     /locus_tag="PWS53_001243"
     CDS             3931..5946
                     /locus_tag="PWS53_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL0871502.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6029..7402)
                     /locus_tag="PWS53_001244"
     CDS             complement(6029..7402)
                     /locus_tag="PWS53_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0871503.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7428..7712)
                     /locus_tag="PWS53_001245"
     CDS             complement(7428..7712)
                     /locus_tag="PWS53_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0871504.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7714..9729)
                     /locus_tag="PWS53_001246"
     CDS             complement(7714..9729)
                     /locus_tag="PWS53_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0871505.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9908..10213)
                     /locus_tag="PWS53_001247"
     CDS             complement(9908..10213)
                     /locus_tag="PWS53_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL0871506.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10302..11333)
                     /locus_tag="PWS53_001248"
     CDS             complement(10302..11333)
                     /locus_tag="PWS53_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0871507.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11540..12415
                     /locus_tag="PWS53_001249"
     CDS             11540..12415
                     /locus_tag="PWS53_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0871508.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12567..13409)
                     /locus_tag="PWS53_001250"
     CDS             complement(12567..13409)
                     /locus_tag="PWS53_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0871509.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13501..15378)
                     /locus_tag="PWS53_001251"
     CDS             complement(13501..15378)
                     /locus_tag="PWS53_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0871510.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15375..16796)
                     /locus_tag="PWS53_001252"
     CDS             complement(15375..16796)
                     /locus_tag="PWS53_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0871511.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16799..20188)
                     /locus_tag="PWS53_001253"
     CDS             complement(16799..20188)
                     /locus_tag="PWS53_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL0871512.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20445..21836
                     /locus_tag="PWS53_001254"
     CDS             20445..21836
                     /locus_tag="PWS53_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871513.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21909..22556)
                     /locus_tag="PWS53_001255"
     CDS             complement(21909..22556)
                     /locus_tag="PWS53_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0871514.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22564..23250)
                     /locus_tag="PWS53_001256"
     CDS             complement(22564..23250)
                     /locus_tag="PWS53_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871515.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23256..24329)
                     /locus_tag="PWS53_001257"
     CDS             complement(23256..24329)
                     /locus_tag="PWS53_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871516.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24551..25225
                     /locus_tag="PWS53_001258"
     CDS             24551..25225
                     /locus_tag="PWS53_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871517.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25230..26570
                     /locus_tag="PWS53_001259"
     CDS             25230..26570
                     /locus_tag="PWS53_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0871518.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26719..27705)
                     /locus_tag="PWS53_001260"
     CDS             complement(26719..27705)
                     /locus_tag="PWS53_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL0871519.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8079 a         5248 c         5035 g         9364 t
ORIGIN      
        1 ccagtttctt tattcagtta atcaaatatg aacgaattag atatgaatca ttgtatttgc
       61 cgttgcggaa ggatttttta attaaccaca gataaacatt gataacgatt cgcttattgt
      121 aatcaacaga ttaaatttca tatgttcata tgtacggata cattttcgga ccgattaatt
      181 ttaaccattc tgtttatttg cttacctatt agatatcaat aaaaaactgc ttaacagcta
      241 tttcagctgt taagcagttt taggattttt agttcatatt cattgtttct tataatacag
      301 tgttcaatac ttctcttaaa gcacttgttg atttgctgaa cattgctttt tcttcttcgt
      361 tgattgataa ttcaacaact tcacgaacac cattttcgtc aacgattgat ggcacacctg
      421 taaagatatc tttttcgtcg tattcaccag ttaaataagc agatacaggc aatacagctt
      481 gttcgttatt caagatagct tttacgatac gtgcagtact cataccaata ccatagtaag
      541 ttgctttttt acgatcaatg atctcataag cagcattacg gactttatca gcaatcacat
      601 ctaattcatc ttgggcaata cgatggtctt tttctacaat ttcaaacact ggtttaccac
      661 cgacagttgt atgtgaccat gcagcaactt ctgaatcacc atgttcacct aagatatagc
      721 cgtggacgct gcgtgggtca acttttaatt tcaatgcgat ttctttacgg aaacgagttg
      781 tatccaatgt tgttccagta ccgataacac gtgatgttgg cagaccagat tcgttccaag
      841 cgatatatgt caaaatatct actgggtttg aagcaacaac gataataccg tcgaatcctg
      901 atcccatgat ttcttttacg atttggcgca tgattgacgc atttgtttta actaaatcta
      961 aacgagattg accaggtttt tggttgatac cagctgtgat aacaacgata tcagcatctt
     1021 tacattcttc atagccacct gaccatacag ccacattttc atcgccccaa gccataccat
     1081 ctaaaaggtc caatgcttcc ccttctgctt tttcttgatt cacatcgatc aatactaatt
     1141 cattagagat tccttggttg atcattgcat aagcgatact tgttccaaca aaacctgtac
     1201 cgacgattac tactttacga cttgtttttt tcatgaataa acagctcctt ttcataacta
     1261 tttgttactt tcatgaaaag tatagtcttg tttattcatt ttatcaacat gttaattata
     1321 atagttgtta ttataatttt tgatggaaac gaattcctga atacgcataa cagataaatg
     1381 acaaatgttt acacctatat cccctgataa tacaaccaaa agtttagaac tgaaaataaa
     1441 tccaaaaaaa ttcgctgcaa cgatataata agacagcaat cgcacctaaa acaattttcc
     1501 ttcatatgct ttagtgaaag attttcttct taatgtttat ttctccattc attgggagta
     1561 catgttgaat acttcttgaa taatttgaat aaagaagtcg tttgtttata tccgatacga
     1621 ttagcgattt cttccaacga aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat
     1681 tttttcttta gaaccagttc ttgaaaggaa gcatcaaaat acattttcaa tttattacta
     1741 agatagaccg gattgtagcc aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa
     1801 tgcttatcga tataatctaa tactctaagc atctcatttt ctgcgcatcc atctgctgat
     1861 gaaaagcttt gttgttctaa taaatgagtc aattcgatca gtaatagtga tgtatagaga
     1921 tttaacgaac ggatcttatg actgtatgta tcaaaataaa tcaacagcat attttcaatc
     1981 aaatgcgtca aacgacttgt ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct
     2041 ttttcttttg atgcatttat caaaaaagaa gaaagtagat tatttgtgct tgccaaatga
     2101 ttcaatatct ctgtagataa ggtttcttct tttaataaga tgttgattaa aatatcctct
     2161 tgccctgtgt attcgattct ttgagtagct tctttatcaa tgatcaccac gtcactttct
     2221 tccatctcta tcctttctcc attaataatt tgtaaacatg taccgctata catataattg
     2281 atttcaacaa aatcatgtgt atgctcggga acttccgcat atcgacggtg tttactgata
     2341 tgaatatttc cctgttgaaa tagatcccct cggtggattt ttggcggtgt ctgaagatgg
     2401 taagctaagt ttaaatcatt gatattcatt ccttgttcac gctgcagagt ttcgattgat
     2461 tcttcgttac gcaaaaaaaa tgtctaattc agctagttcc aattgactcc ctcctttttg
     2521 taagcggttt ctttcgctta ttagttcacg ctgtactgct attatatgca aagctatttt
     2581 tggtttcata aagactttct atggttggga catgaacttc ctgtgattcc gctattgaga
     2641 gaataaccac ttcatactat atttttgata agcgcttaca aaaatgaaag gaacatgctt
     2701 atgaataaaa agatgatgaa attttctctg ctgtcagtct ctcttatttt gacttctgct
     2761 ggtgcagtca cgtctgcaat tcccagtatg tcagctactt atgccacgcg ttctttatct
     2821 gctgttgaat tattaacgac tgctccctct tttatggtca ctatttttgt tttgattagt
     2881 agttttattg ctaggaaaat cggacaaaaa caaactgtga tcatcggatt agtgattgca
     2941 tcgatttgtg gtatcctccc cctcttctca caaagttaca gtattgtctt agcttcaaga
     3001 gctggactag gtattggctt tggattgatc aattctttgg cagtcagcat gattgctgcc
     3061 ttttatcacg gagatgaacg agcctctatg attggttttc aaagtgcatt tcaaggattt
     3121 ggtgcagctt taatgacatt tgttgcagga cagctagtac agttcggttg gcaatatacc
     3181 tttttagtct acgccatcag cttacctatt cttgtcttgt ttgttttgtt tgttcctaca
     3241 cctgaaacaa ctgaacttca atcgcatggc acagccccaa caagacaagt aatcaaaacg
     3301 aatcctaaaa tgattagtta tggtattttt ctcttgctgg tcatcgtggt atataatgct
     3361 gtatcgatta aattagccac agtcctgacg acttattctc taggaaacga aactaatgca
     3421 gcgaatatct taacaatcat gcaattggca agtatggctg ccggagtttg ttttggctgg
     3481 cttcagacaa aagcacatcg ctatatgctt acactttctt tactactgat gggaatcggc
     3541 tttgtattga ttgccactgt gataaacctg tatgtgattg ggtttggtgc gatcctaaca
     3601 ggaatttctt tttcgttatt cattccttac ttattcaacc aagtaagtat ccaagcgccc
     3661 aagtacgcgg agagtttcaa tacttcaatc ttattggttg gcgctaattt aggtactttc
     3721 attgcaccat atggtttgct ctttctctct tttgcttctc ttgggagtaa aacacttgct
     3781 gttttcgtca atggcagtat cttgctgttg attagcgcgc ttattagcct tctagtagta
     3841 cacaggacga atagaaagac agtgataaga gtagatgaag aaataaaaga acaattgaca
     3901 acaaaataaa tttgatggag gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg
     3961 ctttggtatc ctggagactt tgagatccgc catggactgc tacaaaattt ccaacgggaa
     4021 gaacgaggtt ttgactggcc agcgtactgg tacatggatg attgccatcg gaacgtcaag
     4081 tttaagagat attactttct tgatcaacct tccatgttta aagtgacgat ccaaggagtt
     4141 gggtatgtag aaattaatgg acaaaaacat ccatgtggaa aatggctgac ttgtccagct
     4201 ggtaaagcaa aaattcgtat ctttgtagga catacatcag ggctaccagc gatgtttatc
     4261 gagggagacg aagtaaaatc agatattggc tggacagcaa gtaattttat tgaagaatat
     4321 ccagctggct ggagtccgct ttatgtcgat atcgcaaaag atccaaatca aatctattat
     4381 caaaaagaat gcgtccatcc agtcactgaa acggagattg gcggaggaat cttattcgat
     4441 tttgggcgag cagtgaatgg ctgtgtagaa ctaatatctc tggatgacca tatcgaatct
     4501 atcactctat gttatggaga gtccgatgta gaagctatgg atatcgatta ttgctactat
     4561 aagcaaatag gaatgacaag aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt
     4621 ataccagagg tttctactgg aatggttcaa cttcaagttt ttcatgaatt tttatctctt
     4681 tcgaaacaag ctgctttttc aagcgattct tctttactga atcagatctg gtcagtatca
     4741 gaagaagcct tcaagttatg tagtgatttg ttttttatcg acggtatcaa acgagatcgc
     4801 tggatttggt ctggagatgc gtatcaatca ttcatgatca atcaatatct tttttttgac
     4861 gaggaaatca ataaacgaac gatattagca ttaagaggac aaaatgaaat caagcagcat
     4921 atgaatacta tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa ccactatatg
     4981 atgacgaaag actatgaatt tttacgcatc gtctacccta agatgcaatc attaatgaga
     5041 tacttgatgg agcaaacgaa tgaattaggg tttatttatg ggagagcaaa agattggata
     5101 tttattgatt ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca agtcttatta
     5161 ctgaaagcct atcattccat gatcacttgc ggagatatct tagctgaaac atatggagag
     5221 aacgaaaatt atcatactca tctctatcaa gaaaaatatc aactattgaa acaaaaccta
     5281 tttgactact tttgggatga agaacttggc gcttttatcg attgctatga atctgggaga
     5341 agaaatgtga cgcgtcatgc gaatattttc gctgttttat tcgattttgc taacaaagac
     5401 caacagcaat cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca gattactact
     5461 ccatatttca aattctatga acaagatgct ttatgcaaac tcggacagac gaatatcgtc
     5521 taccaaacga ttcttgatta ttggggaggg atgttatctc atgatgcggt aactttctgg
     5581 gaagaatacg accctactca aacaggaaat gaaaagtaca gtatgtatgg agatccttat
     5641 ggaaaaagct tatgccatgc atggggagca agcccaatct atcttttagg acgctatttt
     5701 atcggtttgt atccaacaaa gccaggttat gaagaattta tgatacaacc taatctagat
     5761 tcatttgaaa cactgaattg tacgctacct atcaaagatg gaaagatttc gcttgaaaaa
     5821 acaaaagata agatcgtgat tacttctgat agaacaggcg gaacagtgaa aatgaatggt
     5881 aaaagttatc ctcttctagc aaaacagcca cttgtcttaa caagctattc aaaaataaat
     5941 gcataaaaca aaaaactgct tgcgacatat cagaagactt taaatcttct tctgatattg
     6001 tccaagcaat ttttattttg aagtttgctt aattctgttc tttagaaaca atgtttgttc
     6061 tttttccggc aaaaagaaag ctagataata cgacaagtaa aaagaaagcg atgattccaa
     6121 ctgtcaaggc tatttgaccg tattctccta gtttcccaag tacaagtcct ggtactagat
     6181 aatcagtatc ggcaaatgta gaagaagcga ttttaagatc tcctaatact ggaaggataa
     6241 ataatggcag ccaactaatc aataagctat tgaaagcaga acctaatacc gcccctcttc
     6301 taccgccagt cgagttgccg aagacaccag aagcagctcc gcagaagaaa tgtcctacta
     6361 ccccaggaat gataactgta gtttgcaagc ctatcatagc aaacatggat agtgttccta
     6421 caaagaagct aacaaaaaag ccaattaata cagcattagg tgcatatgga aaaacgatag
     6481 gaacatctaa tgctggttta gaatcgggta cgagtttttt agcaatccct tggaatgctg
     6541 gaacgatttc tgccaacacc attcgaaccc cttgaaggac gataacaaac ccagctgtga
     6601 atgttccagc ttggatcagt gcaaagatta cagcgttgtc tccattgctt agttctcttt
     6661 caacaaatcc aggaccagct aacaaagcaa gtgcgacata tacgatcgac atcaagagca
     6721 tgatcgatac agtagaatct cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg
     6781 tagaacggtc tttgtttcca aaaaactttc cgatccatcc actagaagca taaccgatat
     6841 ttccggtatg ccctaacgca acttgattgt ttccagtgat gttcttcata aacggttgac
     6901 aaagagcagg cgtaatagtt aatagtgttc cttcgaagac cccgcctaat aagatcatcc
     6961 acaaatttgc agtaaacccc gcgcttacta aaatgactgc tgttagacaa gaaacataaa
     7021 gcattgcttg acctgtaaga aaaatgaatt tatatcttgt gaatctagca agtaaaatat
     7081 tcacgatcat ccctactagc atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa
     7141 tcgccactac tgcttcattg ctaggaacta ctccttgagt atgcagagct gtttggaaca
     7201 tcgaagcaaa aggggttagt gaattactga ttaaaccagc accgccgatc agtactagga
     7261 atccgacaaa tgttttgata gttccttgga tcacttctga aaatttcttc ttctgaagaa
     7321 cgagaccaag cagtgcaatc aaagaaacga ggatggaggg ctgactgacg attgacacaa
     7381 acaaattcaa caatttatcc attgatagtt cctcccttga atcattttta ttcttttgac
     7441 aagatcttcg ttactttttc ttttacttct tcgctgtcaa tgatcgattg caaaggtacg
     7501 agcttttcat caggtataga agttagcgta tctttcaaag aatgctcaat gaagaaataa
     7561 tcagccatat cgctagatac acttccaaca tccgtatgac ttacttcaaa ctcgtccaca
     7621 tctgctccaa gatctgcaag tgcttgtttg atgttcatct ctaccataaa acttgttcct
     7681 aatccacttt gacatactgc aaaaattttc atatcattcg tctccttttc ttatgatcgc
     7741 taatatttct tcttttgttg atgcgtgtaa taaatcttga agattttgtt tattagaaag
     7801 tagctttgtt aaatttgcca aagcccgaag atgtgcctca ttatcgatag cagctaaaca
     7861 gatgaacaaa ctaatgggat gggctggatt atcaagcaga tccacaggtt gttgaagttt
     7921 cagcaaagac atccctactt cgtttacgcc atcttctgga cgagcatgag gtaatgcgat
     7981 gtgatcgcca atatggataa acgcaccata ctgctccacg cgctcgatca tagctttgat
     8041 ataactttct tctattttat tttctgataa tagcggctgt gcggcaaatg tgattgcttc
     8101 ttgccagttt tttggttgat ccgataattg gatcatttct tctgttaata attcagatag
     8161 cataggacgt tgatcctcct tcttttctaa aagtttgttc attttacgat ttaatacttg
     8221 atagagcttt tcttcgtcta cgccttcttt taattcgata taaggcagta gcgccgacaa
     8281 caattcttgg gcagaaggca ttgaaaatcc tggtatcagc caatctcttt gtacttgatt
     8341 gatcagctga tttttttcca agtcagtcat gattggatgc agaacataga gagacttttt
     8401 cttatcagta agtgtgagag gtacagtaga aaaaataata tcgtaagaag tttcgtcaac
     8461 ctcgtgtaag cggtcagctg aattagtctc tgtaaaaaag atcatcggga ataattgctg
     8521 aagttcactt tttaagatca atgaagagct aatgccactt ggacagacga tcacggctct
     8581 tatttttcta tttctttctt ctgcgtccgc ttttctgatc tctccgccaa atagtatagt
     8641 gaaaaaacca atctcttctt cagggatgct cttccctatt cgtctttcta acggaagtaa
     8701 tgcttttctt gtcatttcgt agatggaagc atattgatgc ttaatttgat cgatcatcac
     8761 attaggtaaa taaaaagaat aggaaatacg aaaataggca gggaccaagt gataaaagac
     8821 attcatcaat aattcacgaa acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg
     8881 gatgacttct gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga catctccttc
     8941 aacgatcgat gctaagcaaa gagagaaaaa tataatctcc gaattttgat gttcggaata
     9001 gccaaggcca gtcaatactt gtcgactaat gtcagtgact gcaagagaag ccaacagttc
     9061 agcatctttt accagttcca tggatgtttt tccaattcga atggttgtca aactcaaata
     9121 ataagctaat tcatccattc gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt
     9181 atctagcaaa tctctaactt tggcgtaata atcaatggaa agggttgcgg ttaaatacgt
     9241 gagcccaaat cttccagcaa gtgactgcat caaacgagca gtgcgatttt ttgcttctct
     9301 tcttaatatt gcttctgacc ctagcagcct aaaaccagtt ttcctagtat acgacagctg
     9361 acaatcttcc acttctaatt catttcttat ctttttgata tctgacagga ctgtcccttt
     9421 agaaacctga agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac
     9481 catcaaataa agaagatctc ttcgctcttg ttcggtatac acagcttcaa atcgagtaat
     9541 atccaaaaat ttttctgccc agtcgattgc tcggaatttc tttatacgga tacgggtatc
     9601 atccacatat tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat ttacttcgat
     9661 gatctctgct gctttttcta gactgatttc tcttggtgtt ttcaggagat aactaatttt
     9721 gtagttcaca gcgctgttca cctcctactt atattctagt atggtagcgc ttttaaatcg
     9781 aggggtgttt ggtacaactt acagttggat tttttttacg actgtaccga acgatttcta
     9841 tcgaatggat acagaaaaaa agcaggtgac caaatgtctc ctgcttttat gaattgattt
     9901 cgtccgatca atgatgatca tgtttatttt ttgaggtcag ttcttcaaat ttttctttga
     9961 catcttcaac cgctttttcg atatcttttt caaagtgatt gggctcatta ggctttgttt
    10021 cctcttctac agaatttgaa ccagataaag ctgtttcttt attttctgtt tcaccagagt
    10081 ttgtgccagc taatgattct tctttgtctt ctgtttcacc agaattagtt cctgctaaag
    10141 ccacttcttc ttctgtttta ggggccattg aacctcttaa tcgtttgtgt ttttcaccgt
    10201 cataatttgt catgttggtt cctcctagta gttctatact ttttcatcgt acaataagac
    10261 agtttatgcg tcaaaaaaca aacccgattc ttacaaatat gtcatatttt tccttctgat
    10321 tcttgaatgc ttttgattcc ttcttccaaa attttcattt gtaagattgt ttcttcatct
    10381 ttgatgactt tttctgcgcc attaactaat gtctcgtaaa tcccttcgta gacacggctg
    10441 taatcaccaa cctcggaaat tactttttct tcatgataac tcccttgttc atctatatag
    10501 gtcaatacac cataatgttc gggcaaatcg ataccaaagt cttcatgatc aggcatgtaa
    10561 aacattttca gatgttcttc ttgtcgatct tttgtttgtt tcacgaacat cccttttttt
    10621 ccgtaaacga cgaaacttgg acgttctttg atgcggaaat aactggattt tatcgaaatt
    10681 ttcatatttc catagaaaaa atccagatcg aagtaatcat tcattcgtcc tttccctaaa
    10741 agttgacgta catcatagct gatatcatct ggttcgccga aataagaaag aacttgatct
    10801 actgtatgac aagcatgtcc atataaatag ctgttcgcag tagaaaattc atgcgtattt
    10861 tctggtactt ccggtctgaa ataatcataa tgcatttcta cttctaaaat atcccctaat
    10921 tttccgcttt cgattacttt ttggactgtc aagaagtcag aatcgaaccg tctgttttgg
    10981 taacattgga taaatagacc tttttctttt gctaatgcaa ataactcttc tgcttccttt
    11041 gacgtttctg caaaaggttt ctctactagt acattcttac catgttcgag tgccattttt
    11101 ccatattcat aatgaaattg actaggtgta gtgatgacaa ctaattggat ctcaggatca
    11161 tcccaaagat cagacagctg atcagtatat tcgatgtctt cgaatgtttt ccacactggt
    11221 tcagatgggg aaaaaatttt tttgatattg attttgttcg taagttttct tgaaaaaggc
    11281 aaatgatagc gatttgtact ctttccattg ccaagataag cgattgtaag catgagtggt
    11341 gcctcctttt attgatgttt tcagtatatc ctagaaaagc gcagaaagta tttataatag
    11401 ctattctggt aaaaaagttc agaaaaacac ttgttatcat tcggtttggt gaaattaatg
    11461 ttcaaagtaa ggataaaaat caaaaagata gtagcggaac caagtgttta tcagtaagat
    11521 agaatttgga ggtcagatca tgcttattga agaaaaacta acaaaacaag agttgtttac
    11581 cacgacagag aaacggattg tcgattacat ccgtaggaat atcgaagctg cagtctacat
    11641 gacgattgaa gaattagcaa aagctacgta tacttcccat tcagctatca ttcgtttatg
    11701 caaaaaaatg ggattcagcg gatttaaaga attccgtttc gagctggggc gggaagttca
    11761 tcaattgatt gctcagttca atcaaacgat tgatcctaat tttccttttt caagtgaaga
    11821 tagcactcag caaattgcaa agaaaatggc ggatttatcg atccagtcaa taaaaaaagc
    11881 acaattgcag attgaacatg aagatttaga tcaaatcggt aaagtattga acaaggcaca
    11941 gcgaatcttt ctttttgcaa aaggtgattc tcaaatcacg gcaaggaaat tccagaataa
    12001 aatggtcaag ttgaataagt ttttgatcat ggctgaagaa tacagtgatt cttcttggaa
    12061 tgcagccaat ttgactagcg aggattgcgc catttttatt agctacagcg gtcgcatcca
    12121 tcattatgaa cgaataatga cctatttgaa gcatgtagag gcccctacct tgctgattac
    12181 cggaaatcag cattcagaaa tggccaaaca ggctgatatg tgtcttgtta tatcgcagga
    12241 ggaatatgat tttgctaaag tagcgacttt ctcctctcaa attgccttcg attacgtatt
    12301 aaatacgtta ttctcagtga tatattctca aaattttgaa gagaatatcc gcaacttgaa
    12361 aaacaaacaa gaattgatgc aaaaaggcct tcttaaagaa tatcggaaaa cttaagaaaa
    12421 gatcctcatt tagtaataag aaagatagtg tttgataggc tctctttgat atcatcataa
    12481 aaacaggctg aagttttctt tttcaaacct cagcctgttt gtttacggaa cgattcgaat
    12541 agatatatta tagaaatgag gaatagctaa cttctgtttc ttattttgaa cgattgacga
    12601 aaaagatata taagaaggag gaaaatcagt atacatgcag ctaacaatcc atacaggaaa
    12661 aatcgtttgt tctcggatac tttctgtact tctttttgaa cttcttttgc atgataagga
    12721 acccggtgcc ctctgacaag taagcgatga ctgttgacca tataaggtgt acaagttagg
    12781 agtgtcacga aatcctgccc tttttcgatt ctcagatctt ttgtatcagt cggttctacc
    12841 gtcttgatct gatcgacttg ataagcgagt gtccgtccat tgatttcgat atagaaagaa
    12901 tcacctaaag tcagtttcgg taagtctgtg aacaactttg cttgaggcag tcctcgatgg
    12961 ctggaaataa cggcatgtgt attttctccc cccgtaggat aagacgttcc ttccaataat
    13021 gaggcacctt tttctagcag cacagtattc gtttcatcaa aaattggcaa atggacattg
    13081 atctcaggga tcgtcaaaat accgatcgta tgtttctcaa aataggttct atcctttttc
    13141 gcaggttctt ttttcttagt aaagggatct gcacctggat ttccaccttc ttttgctaat
    13201 tgctggtttt tctttgtcat gttttcttgg attttagcca tgacttcttc attttctttg
    13261 accgcttgtt gctgataatg agagatgatc tgctgatcca gataattatt cagcgcgtcg
    13321 ctaacaaatg gataagctag tatccctatt cctgagagaa gcaagaaaag catcagcaga
    13381 tctagaagtt tccttttctt tggtttcatt tgtgttctcc ttgtgatgac tgtttgataa
    13441 taaaagaaaa aggagcattt gcgtaatccg ctgctccttt ttccaattct tgcaaaaaga
    13501 ctaaatctga ctgtgcttgc gtctagcaaa gtacagtcca gcaatcagta gaaggactac
    13561 tcctgcaccg atatacacat agattccctt accgcctgtt gaaggaagtg tacctttgtg
    13621 tttatttggt attttttcag gagaaatcaa ctgtcctgct gttacataag attgttcatc
    13681 gatagtaaat gctacacggt ttgttaatgg aacatatttt tctggcgctt tcgtttcttc
    13741 cagatagtac gtgccatatt ttagacctgt cacatcgatt aaaccgttac ttgtggttgt
    13801 aaaaaccgtt gctgattctt tcgccgatac ccagctgacg gcttttgtgg atgggtcgat
    13861 cgataaatat ttcgctgtgt cactatcttg atcacgaacg acgaattctg ctccagcaag
    13921 tgtttggtct gatgtaacgt caccatctac tttaacaaat cgtttgcccc cagtaacgac
    13981 atcgactgac ggtggtgttt gatcatttgt atgcccgtta tcgacatttg cttggttaga
    14041 aaatcctttg gttggatctg ctttttcgtt caaatgcata tagtaaacga atttcaatgt
    14101 accgccagga gttaatgaag gaatataatt cggatcaact gaaaccgtga atccgtctgt
    14161 ttgctcagtg acagaataat ttactgggtc gatttcttta tttccatcat ataaggcata
    14221 agcatacgtt cctgaagaat cattatcaaa tgttaaagca gcgtcatgag tatcgataag
    14281 tttgaatgtt gtgtacttgt tttgcgttcc ttctttatca gcgataccta atgggatatt
    14341 gacagaaatc tcatattgaa ttttttcacc gattgcgaca tcttttccat tcaattgagg
    14401 tgttgtttta tcaactttag acgtatcatt tttgatggtc ttttctactg gtgtttggct
    14461 atttgccagg atctcaaaag gcgtgattgt ttgcttttca atcatttccg cattatcagg
    14521 agcttttact tcttctaaat tatattttcc aacttctaaa tgattaacga tcaactggcc
    14581 tttttcaata gatgcctcgg caaagtcatt gttgccgatg tcataagaat gaccagtaat
    14641 gaaatgtttg gctttggttt gatcagttgt ccaagtgtac aatccatctg tgacactttg
    14701 gatgtatttt ttgacgcttg gtgttccttc ttctttagaa ataataaatt ctgctccatt
    14761 tagtgcttcg ttttcggcag taccgatttt tgtaactttc aacgttccat cattaccgac
    14821 tgtattttta gggtagagat ggatagtatc tagttcttct gtcccgtatt tataagagcc
    14881 atctgcttgt ttgatcatct catatacagg gaaagctaaa accatgtttg cggcagctga
    14941 cactccgtct tttggttctt ctttgatcgt gtagactgca tctttcccat tttgtttttt
    15001 aggtaaagat aaagtgacat ttccatcagc atctgtcgtt cctgaagcaa ctggtgtacc
    15061 aggagtcaaa gactggactg cttgttttgc tgcatccacg gacgctcctt tatctcgttg
    15121 cgcataaaat tcttgagtga cgttataaac tgaaaatgaa atatcggcta atccttggta
    15181 ttgatcgaat tcgctcattt ctttcccgct gttttggatt aaaggatcgg gtaaatcagt
    15241 cattttcttt ttatgaagga tgacttgagc tgctgtctcc tctgcaaaca ctttttttgc
    15301 gccgaagctg tttgtgagta atggtaaaat aaggaaaagg actcctaaca taacgtttat
    15361 ttttttatga tttttcattt tttcactcct gttctttttt tgataaacaa ccagattgca
    15421 aagcttgcga ataccatacc aacaacgcct aaaagtgtat ttttcgtttc gtttgttttt
    15481 ggaagaaatg gacggctatt ttctttttta ttgactactt tcaatatact gtccgcctct
    15541 ttatcctcag aaagaaaagt tgaaatggta aacggaattt ctgtttcact taaaatataa
    15601 ccagaaggtg cttcgatctc ttttaatcga taacttcctg ttttcaaccc ggaaattgaa
    15661 aaagcgccat ttttatcgga aatcaggtga agcgcatctg attcgttctt tgtccacttg
    15721 tatccgttgg ctgtttgctg gaggtaattt cctgctttgt ttttaataag gaatactgcg
    15781 ccagctaatc gtttatctgg tttatttgtc tcgattttga caaattgctt actccctgta
    15841 tatacagaga ctttctttgt actgacacca aaaccaaaat ccagtgttcc gttgttgttg
    15901 atttccttgt tcgcctgtgc tgtactgctt agctgcatct gataagaaat ggtcagtttt
    15961 tttccagcat atttttccaa cgaagggatc gaaaataaaa catcaaatcc gtgaggtttc
    16021 ttgtgtatga caaagccttc tgtcagcttt tctccattcg atgaaatcgt taaactatta
    16081 ggtaaaaacg ttaatgcttc atccgcacta tctgacaatt cgaacttttg atagtcaagg
    16141 atatttacag gaattgtcgt atgcaaggaa taagtgattt tttcaccaat cgtaaaatca
    16201 tttctaggct cttctaatac cttctcaaaa ggtggatcag ggtagtcgtt ttcttcattt
    16261 ttaggataca aatggatagt tgaaagtttt tgattgtttt gtccatgtac aggaagaaca
    16321 actaccatat tctctgcctt ttctttgacg acttctggta ctttggattc aatgaaaaga
    16381 tacgctttat ctcttttttc ttgatcttta gcggccagag aaaaagaagc gataccatct
    16441 tcgttgttaa gagttgtagt tgttgcttct gcggtaaaca taccggaaga cgcaccgttt
    16501 tttgcgatct ctgcttgtgc ttcttctacc gttttgccct tttcccgtag atggtaaaaa
    16561 gaatctgtga catcataaac ttggaatgtg acaccattta atcctcgata cgtttgtaat
    16621 aaagcttttt cttgtccgtc atttggatga tttttcggca gttgcccgtt tgggaaaagc
    16681 aatttgtgta aagtaatctg aaccatttct gtttctgttt ctgttgctac ctgagtaaaa
    16741 gctggtttaa atagtaacaa gaagagacac atactaagcc aaccaagttt tttcatattt
    16801 agctgccccc tctttctttt ttcaaaaaaa gataccaaat agaaaacgca caaccaatca
    16861 tccctactag atagattcct aaacggccaa ttcctcccgt ttcaggtaat ggtacttttg
    16921 cctgattcgt gatgtctaaa gaaatctggt tgtttttggc tcctggtgac agaacagatt
    16981 catgatcttg tccatccact tgaattgacc catcttcgtg tataactata gtaactggct
    17041 cttttagacc ttggtatcct tctggtgtaa aagtttcggt cagcgtataa gttccaggtt
    17101 ttaaattttc gaatagaaag gtatctgttt cttgtccatc ttttggcgat tctaagtcca
    17161 tttctggtcc ctgcagtctg aattttgctc cttttaatgt ctgtccgttg tcttcttttt
    17221 tgtgtaccgt tagatcaaac gcttttagtc gattttgatg agtgagcgtc catacattat
    17281 ttgtcatctc ggtatttttt cccgtgtaaa tgatttttcc atatttatcg atttggaatt
    17341 cataatttcc cggcgaagta tcatatccgg caggaccagc tgtttcaacg actcgatatt
    17401 taccaggtgt gagtgaatca aaactggcaa gacctttttc attcgttgtt tggctgtcaa
    17461 ttggctgata agtaccattt gcttcttttt tctgtaggga aaatgctgct cctttaagat
    17521 ttatctcttt gtccgtccct tgcatcgcat acttacgtac tgctacagga acttcaacaa
    17581 aaggattttc aatagtcaac tggatcgtat catcggaagt agtgactgtt tttccatcaa
    17641 tggttaccgt accatcagaa gcgatcttga tttcccaagt agtctttttg cttaatccat
    17701 gcccttctgg agcttttgtt tctgtcagcg tataggtcat ttctttttgc aatttgacat
    17761 tttctggaag agaataggtt ccgtcgccat gatctgttag taaagtatca atagaatctc
    17821 ctgttaattt gaaaacagcg ccaataagat ccttttcacc tgtagaggaa tttttcgtta
    17881 ttttcaagtt taacggtgtg aattgtttag tatttttcca tgtcattgca tctatttgtt
    17941 gagaatcgta tcctggtaca ggtaattctt tgattgtttg gtaactgaat gcttgacctt
    18001 gattattgta ttgaggtagt gataatatct cttgataact ttctccgcta tttgcagata
    18061 atttgtcaat gtctgcacgt tcccacgtat ttgttgtatc tttagctggt ttaatgattc
    18121 gaatatatcc gtttttccaa gctgcagcat ttgtcgtatg ttcccgttga atctcaaaag
    18181 taacttgatc tggacgatca gctagattgt tgtcaaattc ttcccataac tttttgatgt
    18241 gaagactgac tccgggagct ttagcagatg gtataccgaa ttcagctaat tcattggtat
    18301 caatgtttgg ctggaaagtt gtcctgccgt tcatttgata ccagaaattt ggatggaagt
    18361 cctcattttc tgtttggatt ctcacttggt aatggatttg gatttcttgg tcttttccta
    18421 aatagatctg attgctcttg atggtatttc cttctatgga aatagatgga gtgaccgttg
    18481 taggacttgt ccccacactc ttgactgaaa gtgtaccagg ctgataaatg aatggttctg
    18541 cgattggatc atttatttgt ccattgctta cagttgctga gatctgtaca gcttttttgg
    18601 ctaggtattc agagatatct gttgcatgat cagctgattc atagtaaaga tcgccttttt
    18661 catctgatga aaccatttgt cgcatacgag actctacttc tgcttttgag agaccagctg
    18721 ccggatcgct ttgaagttgg atgccaagac catgtatttc gattcctcgt tctttgagtg
    18781 ccatcgcttc tccgatggtt gcgataaacg tactgtcgat tcttctggat agattgtttt
    18841 ggtcaggtgc atcatagatt cttgagatta gagaagtatt tcccggccga tcttgcgtat
    18901 tagaaaactg agttccgtaa taattgctgc ttgattgtgc gtgtacccgc tgtactttat
    18961 aggaaaatgt tggtacacca tccgtcagca aaacgatcac ttttttatgt ccatttggaa
    19021 cggatagcat gcttcctgca tctcttagtg ctttttgtgt aaaagtacca ccatttgtcc
    19081 gtgaaggtgt aatggatttt acttgatttt tcactgaatc aaatgaaccc atctgtactg
    19141 caccgttact gtagctatat ccttcgcttg agtagccgac atatcccata ttgattttgt
    19201 ctgtgatacc gctatctgct aaagtatcga caaaacgatc gacaccaatc tttacttcac
    19261 cgatccgatt attgtcgttc atacttcctg accagtctac gaccaatacg agatcaagag
    19321 gcgtgatatc tttttgtaca tttccacgag cattcaaata aacatcaaac aatccgggtg
    19381 tacttgtttc ctttgcatac tttcgtatcg catagtctgc ttgattaccg gttcctccat
    19441 attcgatata cgaatgggtc cgatcattag gatctccatt ccaagaattt ataccatccc
    19501 attgatttgt tccgttttta ttgccttgat gatcaagaac atttgtattt cctgtaggtt
    19561 gccaactggc tttaggataa gtgccctgat catctgttgt atattctgta ggaatcgttt
    19621 gattagatgg cacactcaca gctggcaagg ataatgtagg caatgtaaat gggctgacag
    19681 aattttcgtt ttcttttgat gattcttctt ctatttcgtc tggctttggt actgacacat
    19741 ctgtatcttt cttattttct gtctcatcaa taatgtcata ttctttatta tcgacgatca
    19801 gcttgtttgc ctggctgtgg taaagtgctg gatcgattgg aaaactcaga tcgatagttt
    19861 gtttagtgtt tgatgagatt tccaacgtaa ttttcccgct atttacctga taagcatagc
    19921 catctccttc ttttctttcc acatcactta agtaattttc gactagacca atttcttttt
    19981 ctatcttttc agcactggtg ttattcgcat ttagcttgat gttcagtgct ttttttccag
    20041 actgatccga aatgacttca gctgaaattg tttctaaaac tgttggatca tctgtagtgt
    20101 ctgctattgc agataatggc acaaagtttg ataaactcaa tattatcagc atgaaaataa
    20161 ctttcagttt cttccctgtg gtcgtcattt ttcccctcct ttttcttttt ttctgttttg
    20221 acgtgaatag tgaaaattat taaaataaac aggcgttaca taattttatt tattttaata
    20281 tgataattat tatacgaagg gcgctaaagg gaatcacacc aattttcata ccaattggtt
    20341 tcatttatga agttgattcc ttcgattaat gagcgtttca ttgaaagaaa agggttgagg
    20401 gagaaaaaca tatgatcagg ttgttggaag gaaactatag aaagatggaa aaaatcattc
    20461 gaattctgtc catttctgac aagtggatga ctaaaaaaga acttgccaaa cgaattggta
    20521 gttcagaatc tacatttgta aggtatatcg aagagatcaa aatccgctgg ggaacggatg
    20581 taacgattca atcttcacaa aaattggggt atcgtcttga acatttgaat gtaccagctt
    20641 atttaaatgt tctgatggaa atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga
    20701 ttattgtcaa tccgggtaaa acgattgagt attattgtga aaaaatttcc atcagccggt
    20761 cttcttttac acgtaagttg aaacaatgca atcaaatttt agaaagctat ttattaaaag
    20821 tgatcgttga ccaaggtttt cagttaacta gccttaaaag tgagctccag ttgcgtatct
    20881 ttgttacctt tttcttttta agttactacg gaagacgcac gttgccattt ccaatcgaaa
    20941 aaaagaagat caaagaaatt atggtcaaga ataattgtca gatgaatttg tttcaggaag
    21001 aaagcccata tgagcaatct ttctttatta tgtattttgt tgtgcatctc atgagagaat
    21061 cccaaggaac tccatttgaa tggaaagctg gagactctta tgtacagttg ataaatatcg
    21121 ataaaaaaga cttcttaatc ttgaaaaatc tcttaggaaa attgaagtac agcagctata
    21181 taaaagtagt gaactatttt gtcaatacga ttttttcaga attttcggag aaagaaaaaa
    21241 atgaaatcag tgagcagata cgaaaaaata tgtcctactg ttcgttttca tccgctgatt
    21301 gcaaagaaaa aaacgaagaa tatgttgttc aattattaac gcaaatttat tttttctccc
    21361 gtattttccc atacgatacg actcatttga cgatccgtgc aacttacttt gcacagcaga
    21421 tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc agaacttcaa ttttattcca
    21481 ctctactgaa tcagaatctg atttattatt tatctagcta tatcttttgg atgcttatcg
    21541 gtaatcctga aattcaccat tatacttctg ataaaaaaat actgattatc agcgacttaa
    21601 gtcttcgaca tagtcaatat attgaagaat atatcagtga tattcttgct gtgcataaaa
    21661 ttcactctga gacaactgct attactgaag atcagctgtc gaaatacaat cttctcgagt
    21721 atgatctgat cgttacgaat caacccattt tgaatgccca tgttcctcat atattgattg
    21781 atgatagtgt gtcattcgca aatgaagaag aactcatccg cttattcgag ttatgaataa
    21841 tcaaaaaaca gctatgtata aaccaccctg ataaaaattt caggacaaat acacatagct
    21901 gtttcttttt attgtattcc ttcagcaatc caacgagatt tatagcgaaa gtcttttaac
    21961 aactcttctt ttgtccaatt atttgcaatg agatccgtca aagtgttcat tacgagggtc
    22021 atggaaaagt ataggaaact aactgcttct tccgacgatt gaatggagaa atgattgatt
    22081 cttaataatt ccagccattt ttcgttcatt ggagaatcag ccgatagttc tctacgtttg
    22141 gttgtctgct gatcatttcg ctgggttaac aagtataacc tttgccaata gatactttcg
    22201 tgatctaagt cgcagcacca ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg
    22261 ttttcgctaa tgaattttaa gatatcttga tggactatgt gagaactgct tttgatcgta
    22321 tacaaataag catcttccaa atcttgaaaa tatttataaa aagcaccgcg cgacatcccc
    22381 atcttgtcaa cgatttctga gactttgact tggctgatgt gctgtgagca aaatatctcc
    22441 agtaataggt catcgattct ctgtttcttt gcgttcgata aattgaaaaa agtttctttt
    22501 ggcatccaca tctctccttt atctctgatt atacgtggat agcccttcat tgtcatgaag
    22561 gattcattcc ttctttttct cttcttctgt caagtaccca tcttccattc gataaacgcg
    22621 atcactccat tgaatcatac ggacatcatg agtaaccata atagtcgctt tttgtttctc
    22681 atgagcttct ttaactagta atttgacgac ttcatatgca tgatctgtat ccaaactagc
    22741 tgttggttcg tctgctaaga tcaagcttgg ctcattatat aaagcgcgag caattgccac
    22801 tctctgtctt tctccaccag aaaggtcttt aggataacta tttttaagtg actggatatc
    22861 taaagattgc agcaaggaat cgatttttgt tgaatcttct ttttttgaag taacttggtc
    22921 aactagttca aattgttcct ttaccgttaa aaaaggaatc aaattagagc tttggagaat
    22981 aaatcctatt tctttaaagc gtaaatctgc tcgttttttt tctggaagct cagttagtga
    23041 gtgaccgttg atcactattt ctccggaagt cggtgtttgc aatcctcctg aaatggtaag
    23101 gaaagtactt ttacctgaac cagaaggacc aatgatgctg acgaattctc cttcctttac
    23161 ataaaaatca attgctttca aagctgagac ctttgtatga ccagaaccaa atgttttggt
    23221 aacttttttc atcgttaata tttttttcat ttttattaac ctcctattgc tgtaattggg
    23281 tctactttgg ttatcgtacg tatcgaaaac aatccgccta gcattgcgac aatgatcaaa
    23341 atcccactat atagcaacca ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa
    23401 atcagactag tcaaataagc aaacaaaaga gcaagcagaa cgcctaccac cccaacaata
    23461 aaggactgtg ctactaaaga tttagcgatg aagaaatttt tgatcccttg tgctttcatt
    23521 actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca
    23581 gcaaccacta agaacaaaaa gtatatcatc gcatttaagg tcatattttg ggcagaatag
    23641 ccaggaatac tttcaatgaa tgtcccaata gttaataact gtagaccctt cgatgctttt
    23701 tctttcgaaa ttttagctga atcctttatt acgatcccat tgatcggctg ttcttcttca
    23761 gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg tatctaggga cgcatagatg
    23821 acgggagaaa ctgtgtaata agtttcagga aagataccga caatcgtcaa aggatcgtcg
    23881 taactgccaa ttttgatcgt atctccaatt ttatagcctg aatcagccag attttgagag
    23941 atgatgattt cattctttgt tttaaattca tgccctttcg tgagttttgg taataaaaat
    24001 gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt tttttccttt gattgctccg
    24061 ctgtacaatc cgattggtgc tttctctcct ccttcaatgt atttcagatc tcctcttgtt
    24121 aattgtgaag ctgcaaatac ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc
    24181 caatcatcga ttgctttttt aaactcttct gaaagtccat tcgctagtcc tgacaacatg
    24241 aatacgacat acgcaatcaa gaacataata ccgacgatca agccatatcg taatttagaa
    24301 tagcgaattt ctttgatagc tagaaacatt tgtttttcct ccctatgata gattgatagt
    24361 gacaaaatgt cacttgttga atagtgtaac tctaaagtga caatttgtca cttgtgttgt
    24421 ttttttctta tcaataaatt gtctaagaca tctttcgata aaaccttaac atgagtcata
    24481 aaaaatttat ttaagcctta tttatgcttt ccatgataaa ctaaagtgac caaatcaatg
    24541 aggtgggaaa atgattaaac tattagtagt agaagacgat aaagtattat ccaataatat
    24601 aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt tatgatggta gtgaaggact
    24661 gttcgagttg gaaagtggag tttatgatct tgcaatcttg gatttgatga tgccggaaat
    24721 gaatggatat gaagtgttag caaaaatacg gaaagaaaaa atacagactc cagtattgat
    24781 tttgactgca aaagatagtt tggaagataa gattgaagga tttgaacgag gtgcagatga
    24841 ttatctaaca aaacctttct atcgagaaga actgatcatg cgcgtgaaag cattattgaa
    24901 acgctcgcta ggtctctttg atgaaaatgt tctgaagtat aaggatatca gttgtgattt
    24961 aaaaagcaat gaagtcttgt ataaagaaca agttttgccc atacaaggaa aagagtttga
    25021 attgctcgtt tattttttac aaaataaggg aatcattttg acaaaagagc agatatttga
    25081 ccggatctgg ggatttgatt ctgatacgac gattacagtt attgaagttt atatgagcaa
    25141 cttacgaaaa catctgcgaa gtactggatt ggataaagaa atcaaaacct tacgtaatgt
    25201 cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga tattacacga aagcaacgta
    25261 gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat cttttttctt ttgggattca
    25321 ttactttaca gatattaaac caatctgcat acagagaaac agatctgtca ttagagaaaa
    25381 taaattttga ttctcgaaca gtacaattag agatacaaag atatcagcaa ggcgatccct
    25441 ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat ggaacagcca ttaatggaac
    25501 catcaaatga ccgttttaac actcaagtga tcttgtggtc taaaaatggt gagatattga
    25561 ataaagcagc tattggtggg cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa
    25621 atctgaatac catccaaaat attgaattaa atgaagacag tggctcatct gccctctctt
    25681 tccgttcgat tacgaaaaaa gcggagacga atcaagatgg cgttgcctac atccaaattt
    25741 tagaaaatac aaatcaaatc aagaattcat tagcgacttt tcgcacgatt atgattttgt
    25801 gtatgattgt tttttggatt ctctctattg gtatcagtta ttacttgtct agtctaagca
    25861 tgcgccctat tattctttct tggagaagac aaaaagaatt tgttgagaat gcttctcatg
    25921 aattgcgcac accgttaacg attattcaaa atagtttgga acatcttttt accaagccta
    25981 atcatactat tattgaagaa tccgaaagca tcgctcaagc attgtcagag acgaggcgcc
    26041 tcactggatt gacttcggat ttgctgacaa tcgctcggaa tgattctgat cagcagctgc
    26101 tttcaaagca aatgatcaat acacaagaat atatcgaaca acttgttaaa ccatttcaag
    26161 agatggccat catagatgga aaagagttta ttctccataa cgatgcaaca ttagaagtac
    26221 caattgatga aaaaaaaatc catcaagttt tggttatttt gctggataat gcattaaaat
    26281 atacgaatcc agaagataag atcattttgg attcatctgt taaccataaa gaatggctga
    26341 ttcacgtaaa gaatactggc ccttctatca gtgaagaaga tcaagcaagg atttttgatc
    26401 gattttatcg ggaagatcaa tctcgggcga aagagactgg cggatacggg cttggcttgg
    26461 caattgcaaa acaggtaatc gaacagcatc atggacagat ttctgtatcc aattatcttc
    26521 cacaaggagt cgattttaaa gtccaattgc cgttgaagcc taaaaaataa tgcttatccc
    26581 taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt ttgttgcgaa taaacggtgt
    26641 tccagaagta gcttcttcgg aaataagccg aaatttcttt aagctgacca cttctgtcac
    26701 aacctcttta gcttacgctt acgatttttt attgcggtct tcaatgtgta catcaccgct
    26761 tgtgtcaata tccaattctt ctcggctaac atcttctgtc actttttcaa catcttcatg
    26821 ttcttcttta tgaatcccaa cttcttcacg aataactgta tgtttgctta catctacttg
    26881 ctcttctttg attgggatag taatcgtgtc atcttccaat ttttcgtctg ttccctgtga
    26941 agattgatct gttactggtt tgcgttcgat gacgatttct tcacgtttga ccggtacttc
    27001 taccgtttca gtatcgttga ccacatgttt gtggatgtcg acttctccag ttgtcacatc
    27061 atgtgtattt acatctaatt tttcttcttt tagttgaatt gtatcatcat ctagatttgg
    27121 tttgcgatcg atgtcttcag tcttgcttgt acccattgta tcagtctcgc caggtacgtc
    27181 catcggatca gttcctggtg tcatattcaa tggatccgtt gtattttgaa agcctgtcgt
    27241 gccacctacg tccattggat ctgttccagt gccagtttct aatccagtcc ccattgtgct
    27301 tgctgggcta acagtatctt gtgtatccat tgtatcttct gttgtttctg gctgacgata
    27361 gccttttact gtgatgacaa attttccatc agaaatatct cctctatatt gatataaagg
    27421 atcattttct ggtgtagccg tctcagaatc ataggtatca aaagaaaaag tatcttttat
    27481 tttttcccat aaagaacggt cttcttcacc atccatccga ttttcatctg tttcaacatc
    27541 tataccagac aatccgtcaa atcctagtct agatgctgct tcatcagtag tataaataac
    27601 gatatcatct cgttcatacc cttcgttacg caaccgttca attactgata atgcttcttc
    27661 cctcgtacta tagcttccga tgaccgttaa atttttctct tccatgtaaa ttcctcctag
    27721 caaata
//
LOCUS       DANJJH010000032        25530 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000032 DANJJH010000000
VERSION     DANJJH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="PWS53_001261"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="PWS53_001261"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..279
                     /locus_tag="PWS53_001262"
     tRNA            207..279
                     /locus_tag="PWS53_001262"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Val,seq:tac)
     gene            283..355
                     /locus_tag="PWS53_001263"
     tRNA            283..355
                     /locus_tag="PWS53_001263"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Lys,seq:ttt)
     gene            363..444
                     /locus_tag="PWS53_001264"
     tRNA            363..444
                     /locus_tag="PWS53_001264"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:397..399,aa:Leu,seq:tag)
     gene            462..534
                     /locus_tag="PWS53_001265"
     tRNA            462..534
                     /locus_tag="PWS53_001265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Thr,seq:tgt)
     gene            546..617
                     /locus_tag="PWS53_001266"
     tRNA            546..617
                     /locus_tag="PWS53_001266"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Gly,seq:gcc)
     gene            634..719
                     /locus_tag="PWS53_001267"
     tRNA            634..719
                     /locus_tag="PWS53_001267"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:668..670,aa:Leu,seq:taa)
     gene            730..803
                     /locus_tag="PWS53_001268"
     tRNA            730..803
                     /locus_tag="PWS53_001268"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Arg,seq:acg)
     gene            813..886
                     /locus_tag="PWS53_001269"
     tRNA            813..886
                     /locus_tag="PWS53_001269"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:847..849,aa:Pro,seq:tgg)
     gene            906..978
                     /locus_tag="PWS53_001270"
     tRNA            906..978
                     /locus_tag="PWS53_001270"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:939..941,aa:Ala,seq:tgc)
     gene            1006..1079
                     /locus_tag="PWS53_001271"
     tRNA            1006..1079
                     /locus_tag="PWS53_001271"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1040..1042,aa:Met,seq:cat)
     gene            1093..1166
                     /locus_tag="PWS53_001272"
     tRNA            1093..1166
                     /locus_tag="PWS53_001272"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1127..1129,aa:Ile,seq:cat)
     gene            1190..1279
                     /locus_tag="PWS53_001273"
     tRNA            1190..1279
                     /locus_tag="PWS53_001273"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1226..1228,aa:Ser,seq:tga)
     gene            1292..1365
                     /locus_tag="PWS53_001274"
     tRNA            1292..1365
                     /locus_tag="PWS53_001274"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Met,seq:cat)
     gene            1384..1459
                     /locus_tag="PWS53_001275"
     tRNA            1384..1459
                     /locus_tag="PWS53_001275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Asp,seq:gtc)
     gene            1461..1531
                     /locus_tag="PWS53_001276"
     tRNA            1461..1531
                     /locus_tag="PWS53_001276"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Gly,seq:tcc)
     gene            1545..1618
                     /locus_tag="PWS53_001277"
     tRNA            1545..1618
                     /locus_tag="PWS53_001277"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1579..1581,aa:Ile,seq:gat)
     gene            1643..1730
                     /locus_tag="PWS53_001278"
     tRNA            1643..1730
                     /locus_tag="PWS53_001278"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1677..1679,aa:Ser,seq:gct)
     gene            1752..1823
                     /locus_tag="PWS53_001279"
     tRNA            1752..1823
                     /locus_tag="PWS53_001279"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1785..1787,aa:Glu,seq:ttc)
     gene            1846..1935
                     /locus_tag="PWS53_001280"
     tRNA            1846..1935
                     /locus_tag="PWS53_001280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1882..1884,aa:Ser,seq:tga)
     gene            1948..2021
                     /locus_tag="PWS53_001281"
     tRNA            1948..2021
                     /locus_tag="PWS53_001281"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1982..1984,aa:Met,seq:cat)
     gene            2040..2112
                     /locus_tag="PWS53_001282"
     tRNA            2040..2112
                     /locus_tag="PWS53_001282"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2073..2075,aa:Asp,seq:gtc)
     gene            2116..2188
                     /locus_tag="PWS53_001283"
     tRNA            2116..2188
                     /locus_tag="PWS53_001283"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2149..2151,aa:Phe,seq:gaa)
     gene            2193..2263
                     /locus_tag="PWS53_001284"
     tRNA            2193..2263
                     /locus_tag="PWS53_001284"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2225..2227,aa:Gly,seq:tcc)
     gene            2276..2349
                     /locus_tag="PWS53_001285"
     tRNA            2276..2349
                     /locus_tag="PWS53_001285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2310..2312,aa:Ile,seq:gat)
     gene            2361..2432
                     /locus_tag="PWS53_001286"
     tRNA            2361..2432
                     /locus_tag="PWS53_001286"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2394..2396,aa:Glu,seq:ttc)
     regulatory      2621..2700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2698..3891
                     /locus_tag="PWS53_001287"
     CDS             2698..3891
                     /locus_tag="PWS53_001287"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL0871520.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            4021..5493
                     /locus_tag="PWS53_001288"
     CDS             4021..5493
                     /locus_tag="PWS53_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871521.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5576..6364
                     /locus_tag="PWS53_001289"
     CDS             5576..6364
                     /locus_tag="PWS53_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL0871522.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6431..7099)
                     /locus_tag="PWS53_001290"
     CDS             complement(6431..7099)
                     /locus_tag="PWS53_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL0871523.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7214..8926
                     /locus_tag="PWS53_001291"
     CDS             7214..8926
                     /locus_tag="PWS53_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871524.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8929..10698
                     /locus_tag="PWS53_001292"
     CDS             8929..10698
                     /locus_tag="PWS53_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871525.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10903..11469)
                     /locus_tag="PWS53_001293"
     CDS             complement(10903..11469)
                     /locus_tag="PWS53_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0871526.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11469..12425)
                     /locus_tag="PWS53_001294"
     CDS             complement(11469..12425)
                     /locus_tag="PWS53_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL0871527.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12425..13081)
                     /locus_tag="PWS53_001295"
     CDS             complement(12425..13081)
                     /locus_tag="PWS53_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0871528.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13451..15469
                     /locus_tag="PWS53_001296"
     CDS             13451..15469
                     /locus_tag="PWS53_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0871529.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15539..15745
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15896..18310
                     /locus_tag="PWS53_001297"
     CDS             15896..18310
                     /locus_tag="PWS53_001297"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL0871530.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18658..20211
                     /locus_tag="PWS53_001298"
     CDS             18658..20211
                     /locus_tag="PWS53_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0871531.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20592..22376)
                     /locus_tag="PWS53_001299"
     CDS             complement(20592..22376)
                     /locus_tag="PWS53_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871532.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22391..24112)
                     /locus_tag="PWS53_001300"
     CDS             complement(22391..24112)
                     /locus_tag="PWS53_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871533.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24212..24973
                     /locus_tag="PWS53_001301"
     CDS             24212..24973
                     /locus_tag="PWS53_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0871534.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7904 a         4693 c         5427 g         7506 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgcttaagtt atccatggag gtttagctca gctgggagag catctgcctt
      241 acaagcagag ggtcagcggt tcgatcccgt taacctccat atgagccgtt agctcagttg
      301 gtagagcatc tgacttttaa tcagagggtc acaggttcga gccctgtacg gctcatattt
      361 ttgcgggtgt ggcggaattg gcagacgcac tagatttagg atctagcgcc ttacggcgtg
      421 ggggttcaag tcccttcacc cgcataattt aagttttaca tgccggctta gctcagttgg
      481 tagagcatct gatttgtaat cagagggtcg agggttcaag tcctttagcc ggcatcttta
      541 aacacgcgga aatagctcag tggtagagca ccaccttgcc aaggtggggg tcgcgggttc
      601 gaaccccgtt ttccgctttg ccaaagcaat cgcgccgggg tggcggaact ggcagacgca
      661 caggacttaa aatcctgcgg tgagtgatca ccgtaccggt tcgattccgg tcctcggcat
      721 ttcaaatatg cgcccatagc tcaactggat agagtgtttg actacggatc aaaaggttag
      781 gggttcgact cctcttgggc gcgttaatta aacgggaagt agctcagctt ggtagagcac
      841 ttggtttggg accaaggggt cgcaggttcg aatcctgtct tcccgataat tataaattta
      901 aacacggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag gtcagcggtt
      961 cgatcccgct aggctccatt tcatttaaat aatgaatatc tttttcgcgg tgtagctcag
     1021 ctggctagag cgtccggttc atacccggga ggtcgggggt tcgatcccct ccgccgcgat
     1081 ttttaagagc ctggaccttt agctcagttg gttagagcag acggctcata accgtccggt
     1141 cgtaggttcg agtcctacaa ggtccattgt ggcgttgtaa gtttcatatg gaggattacc
     1201 caagtccggc tgaagggaac ggtcttgaaa accgtcaggc gggtaaaacc gtgcaagggt
     1261 tcgaatccct tatcctcctt tttcatatta tcgcgggatg gagcagtctg gtagctcgtc
     1321 gggctcataa cccgaaggtc gtaggttcaa atcctgctcc cgcaattaaa aactaaaaac
     1381 actggttccg tggtgtaggg gttaacatgc ctgcctgtca cgcaggagat cgcgggttcg
     1441 attcccgtcg gaaccgccat gcgggtgtag tttagtggta aaaccacagc cttccaagct
     1501 gttgtcgcga gttcgattct cgtcacccgc ttttagtttt ttatgggcct atagctcagc
     1561 tggttagagc gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt
     1621 agtacaaaat cttattggtt ttggggaagt actcaagtgg ctgaagaggc gcccctgcta
     1681 agggtgtagg tcgggaaacc ggcgcgaggg ttcaaatccc tccttctccg tttcaatatt
     1741 ttgttttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg gcggtaacac
     1801 gggttcgaat cccgtacggg tcattaattt aatatagttt tacatggagg attacccaag
     1861 tccggctgaa gggaacggtc ttgaaaaccg tcaggcgggt aaaaccgtgc aagggttcga
     1921 atcccttatc ctcctttttc atattatcgc gggatggagc agtctggtag ctcgtcgggc
     1981 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca attaaaaact aaaaacattg
     2041 gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg ggttcgattc
     2101 ccgtcggaac cgtttggctc ggtagctcag ttggtagagc aatggattga agctccatgt
     2161 gtcggcagtt cgattctgtc ccgcgccatt atgcgggtgt agtttagtgg taaaaccaca
     2221 gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gcttttagtt tttatgggcc
     2281 tatagctcag ctggttagag cgcacgcctg ataagcgtga ggtcgatggt tcgagtccat
     2341 ttaggcccat ttattttatt ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
     2401 cggtaacacg ggttcgaatc ccgtacgggt catggaagat actttttgag tatctttttc
     2461 tttttatctg aaagaatgat tttaaaaagc ttagcagagt tatctttatg gatgattgtg
     2521 ctaagctttt ttggacagtt ctaatgtaaa atatttttat attttttaag caaaaaaatg
     2581 aaaaaatacg agcagaaaac tgttttatct gctatacttg gttcgttgca agttcccgaa
     2641 aggatccgca ggacggagat gccttgtaac cgaatttaac tagggggaat cattataatg
     2701 gtagaaagac acttatttac atcagaatct gtttctgaag gacatccaga taaaattgct
     2761 gaccaaatca gtgatgcaat cttggatgca attttaaaac aagatccaac agcacgagta
     2821 gcgtgtgaaa cgtctgtaac aactggtctt gttttagtat ttggagaaat atctacgacg
     2881 gcatatgtag atattcaaaa agtcgtacgg gaaacaataa aagaaattgg ttatacacgt
     2941 gcaaaattcg gatttgatgg agatacagca gctgtattgg ttgcgattga tgaacaatct
     3001 cctgatattg ctcaaggagt tgatgaagca cttgagatcc gggatgaaga taaaaaagac
     3061 gtattagatg agattggtgc tggtgaccaa ggtttaatgt ttgggtttgc tgtagatgaa
     3121 acaccagaat tgatgccttt accaatcgct ttgagtcatc gtctggtacg acgcttggca
     3181 gatttgcgta aatcaaatga attaacgtat ttacgtccag atgcaaaatc tcaagtaacg
     3241 gttgaatatg atgatcaagg acaaccggaa cgcgtagata caatcgttat ttcaacacag
     3301 catgatgatg cagtagataa tgaaacaatt cgtcatgatg tcatcgaaaa agtagtgaag
     3361 gaagttattc cagctgaatt attagatgat caaacgaaat attatatcaa tccgactggc
     3421 cgatttgtca ttggtggtcc tcaaggggat gccggattaa caggaagaaa aatcattgtt
     3481 gatacgtatg gcggttatgc tcgtcatggt ggcggtgctt tttctggtaa agatgcgaca
     3541 aaagttgacc gttctgccag ctatgctgca cgttatattg ctaaaaacat tgttgcagca
     3601 gggcttgctc gcaaagcaga agtacagcta gcttatgcga ttggtgttgc tcaacctgtt
     3661 tcgatctcaa tcaatacttt cggtactgga acggttccgg aagaagaact gattgctgca
     3721 gtgagagaaa acttcgatct tcggccagca ggaatcatcg agatgcttga tttgcgtcgt
     3781 ccgatttaca aacaaacagc tgcttacggt cattttggtc gtacagatgt agatttgcct
     3841 tgggaacata cggacaaagt agatgcgttg aaagcaagtt tagcaaaata agttatattc
     3901 agctggaacg atcgccaagt ggtggttgtt ccagcttttt ttatggcagt taacctcttg
     3961 aaaaagatag taattattta tctattttta gatcctgtca taatgaaagg gaggaataag
     4021 atgaaaaaag aaacaaatgt tactttggtc acagtgagta ttttcattgc aacgttcatg
     4081 acggctattg aaggtacaat cgtaacaacc gctatgccaa cgattgtcgg ttcattacat
     4141 ggaatggaaa tcatgaactg ggtattttcg atttatttgc tgacaaacgc tatgcttact
     4201 ccgatttacg gaaaacttgc ggataaagtg ggacggaaac caatctttat ttttgggaca
     4261 ttattgttta ttatcggttc gttattttgc ggtttatctc aaagcatgat tgggttgatt
     4321 gctgctcgtg cgattcaagg gatgggtgct ggggcaatga tgccagtagc tttgacgatc
     4381 atcggcgatc tttattctat tgaaaagcga gcaaaagttt taggtttgaa tagttcagcc
     4441 tgggggattg ccagtgtttt tggaccgctt gcgggaggat tcatcgttga aacgatcagc
     4501 tggcactgga tcttttttat caatgtgccg atcggaattc tattaattat tttgatttgg
     4561 ctatatttaa atgaaccaaa aatcatacat gagtctaaac caatggatat ttttggtagt
     4621 tttagtttga tggccatgct tgtctcattg ctgttagggt tccaattatt aggagacgaa
     4681 ggaatgagtc cgcttgtttc tgctttgctg ctaggtagta tcgtaatgtt aggtattttt
     4741 gtttttgccg aaaaaagagc taaagatcct gtcatcatga tgcatttatt caaaaatcca
     4801 ctatttgttg tggtcaactt ggctgcagca ttggctagtg gttttttgat gggaatcgat
     4861 gtttatatcc ctatgtggat gcaaggtgta ttagggctga atgcagcaat tggtgggctg
     4921 gtattagcac cattatcact agtctggatg gtcggttctt tccttgcaag caagtggatg
     4981 actcaaatga aaactcattg ggtcttgaga attggactac taatcgcagc tattgggggc
     5041 gcttggttgt atcttatgcc tttcagaagt aactatttat ggttttttgc tatatcaacg
     5101 gtcatcggta taggcttagg tgtgaccatg actacctcga ctgtcagtgc acaaaatagt
     5161 gtatctcgtg accagcttgg tgtagcaact tcttttaata cgttagttcg aacaattggt
     5221 caaacagtca tggtttctat ttttggttta ttgataaatg gggtaactaa acgagaactt
     5281 gcagcagcaa atttaacaaa tgatgcagat atcatgaata aactggtgaa tccacaaacg
     5341 gctaaatata ttgatgcgga tattttgtta ccattaagaa aaattcttta tgatgggtta
     5401 cattccgttt atctagttgg cttgttactc gtgatcattg ctttggtctt aacgcttttc
     5461 gttcatgaaa aaagtggtaa aataaaacag tagtttcggt taagatatgt acgtagccga
     5521 atcattcaaa tttaaagtta ttcaaaaata acttcttact aaggaaggcg aaaatatggc
     5581 atttattcaa gcaaatattt attcgaatgt gttagaaatg gaagtcaatc taaatgtgat
     5641 actaccgcaa acaacaaaaa aggtgattgg caccagttca gagcaagaga caacggatat
     5701 acctgtttta tatttacttc atggaatggg tggcaatcat agtgtatggg aaagacgtac
     5761 gtcgattgaa cgttatgtag caaactacgg attagctgtc atcatgccat ctactgatct
     5821 tggctggtac acagatacag tttatgacat gaagtattgg acattcattg cagaagagct
     5881 acctgagatt tgtcatgaat tattcccgca attaacaaga aaaagagaaa aaacatatgc
     5941 cgctggatta tcgatgggcg gatatggcgc gttaaaacta ggccttgcaa aatcagaaaa
     6001 tttcgcagca gtaatctctc tttcaggcgc tgtttctgta ggaaatcgaa tggatgatct
     6061 tttgatggtt cgtaaagcat ctttttggga agggatcttt ggaccattgg atcaaatcga
     6121 aggttcaaaa aatgatccgc aatacttatt gaaagaacta gtaaaaagcg gaaaaccagt
     6181 tccaagattt tatatggctt gtggggaatc tgattttctt tatcaggcaa atcaggaaat
     6241 ggtatctgct atgcaggaac aaggcttggc ggtcaccttt gaacaaggac ctggcgatca
     6301 taactggatc ttctgggacc aatggattca acgtgcattg gcgtggctaa tggaagaaaa
     6361 ataaaaaaag agtcttaaaa agatagctcc tatagagatc acaaacatag tgatctctat
     6421 aggagctttt ttactgctcg ttttctcttc ttttattttg ttggcataag ccttcaatat
     6481 aggtttgtgc actcgttaga aagagtgctt tttcattcaa ttctgcatgg gactgcatac
     6541 tttctttaaa tccctctaat tgattttcaa ctacataatt acgcataaat agaactgttt
     6601 ctttcaaaaa ttcatttccg tccataattt tttgtcgaag ctgctgttca tgctgctgat
     6661 ccattaactg tccaatcaat aaattgtgga gagaggcaat ggcctgatga ctttgggtga
     6721 ctgaaaatcc ttcacgaaca agtaattgga tcatttgctc caaatgttct aatcgactcg
     6781 gaaaagcggg cacagtattg atttctaatt ctgccccaca agggaaacgt gtataaaggt
     6841 catagtaatt ttccatatac tgcagcaact gttcattcca aggcaaagtt ggatctggaa
     6901 gcgctaattc ttcttccatt gtttcagcca ttatttgcaa gagctgttgt ttgtttttga
     6961 aataccaata taaagcaggt gcttgaacat gaattctgtt tgcgatgttt cgcatggaaa
     7021 gctgcgaaag agcttgtgtt tcagccagga tatcaaaggc ggcctgaatg atgattgctt
     7081 gagaaagctt tatttccatt ttatcaacct cgtgtattgc attatctttt taaacatggt
     7141 aaattgttag tcggtctaat tttacagtgt taaattagtc ctgtcaattt aaacaaaggg
     7201 gtgtaatcaa cgaatgaaat taatcttacg acatgcgaaa aaatataaag tagctgtttt
     7261 tatttccttg ctgtccgtag cagtcatggt tgcagcagca ttatggcaac ctaagttact
     7321 acagcaagta ttggaagcta ttattacaga agataatgat gaaatgaaaa caattggtat
     7381 ttacctgatc agtctggctt tgctaggatt agccgcaggt gtaacgaaca cgattttctc
     7441 tgcaaaagta gctcaaggcg taagtgcaga tatccgagaa gaagcatttc gaaagatcca
     7501 gacattttca tttggaaata tcgaacagtt ctctgctgga aatcttgttg ttcgtctaac
     7561 aaatgatatc acgcaaatcc aaaatttagt gatgatttct ttgcaatcac tttttcgaat
     7621 tccattctta tttattggtg ccttcatttt agctatgatc acgatgccgc aattatggtg
     7681 gatcatcgta gcgcttatcg ttacggtatt tctgattacg gcactttcat ttacaaggat
     7741 gggcaagcac ttcatgatca ttcaaaaact gattgatcgt gtgaatagca ttgcaaaaga
     7801 aaacttacta gggattcgtg tagtgaaatc ttttgtacaa gaagaaaacc aattgaagtc
     7861 attcagcaaa gtaagtgagg acttaacaaa acacaacatc atagtcggta cattattttc
     7921 tgtgatgatc ccgtccttca tgctggctgc caatttggct gtggttgggg cgatcttttt
     7981 tgtgagcgat cttgttaaag atgatcctac gttaattggt ggaattgctt cattcatgaa
     8041 ctacttgatg cagattatga tggcaatcat tattggcgga atgatgatga tgatgacttc
     8101 aagagcagcc gtatcgctga agcggattgc ggaaatttta gatgctgaac cagatcttac
     8161 ttatttagac gttccagaac aagagttgac gggttctgtc acttttgacc atgtttcttt
     8221 tcgttatcct ggtgaggata cagatacgct taaagatatc agcttttcta tcaaacctgg
     8281 agaaatggtt ggtatcgtcg gggcgactgg tgccgggaag tcaacactag cacaattgat
     8341 ccctcgtcta tttgatccga cagaaggcaa agttgaagta ggtggtgtag atcttcgtca
     8401 agtaaatgaa aagagtttaa gacaaacggt ttcctttgtt ttgcaaaaag caattttatt
     8461 ctcaggaacg attgctcaaa acttgcgcca agggaaaaag aatgcaacag aaaaagagat
     8521 ggaacatgct tcttctattg ctcaagcaaa agaattcatc gaaaagttat ctgatcgtta
     8581 cgaagcacca atagaagaac gtagcagtaa cttttctggt ggtcaaaagc aacgtttatc
     8641 cattactcgt ggcgttatcg gtaatccaaa gatcttgatc ctagatgaca gtacgagtgc
     8701 actggatgct cgctctgaaa agctggtccg agaagcactc gatcgagatt tgaagggaac
     8761 aacgacgatt gtcatcgcac aaaaaattgc ttctgttgtc aaagcagacc gcatcttagt
     8821 attggatgaa ggcagattag tcggcgaagg aacccatgag gaattggtag ctggaaaccg
     8881 tgtctatcag gaaatctttg aaactcaaaa aggaacggag gagtaagtat gactgatcta
     8941 ataaaagcaa gtaaattttt ctatcactat ttgaagcggt acaaactctc ctttttcttt
     9001 atctttatca cagtgatcat tgctacctat ctacaagtaa aggcacctca atacatcggg
     9061 gaagcttttc aagaattagc taattacgta ggaggcttga tgcaaggcgt agacgataaa
     9121 agcaaatttg tttctgtaat atggaaactc ttgctgtttt atgtactagc tagtgcagcc
     9181 aactttatct atagtatttt gttcacacaa gtagtaggga agtcaacgaa ccgaatgcgt
     9241 atcggtctat tcaacaaatt ggaaaaattg acgatccgtt tctttgattc tcatcaagac
     9301 ggtgaaattt taagtcgttt taccagtgac ttagataata ttcaaaatag tttgaaccaa
     9361 gctttgcttc aagtcatcac gaatgctgtt ttacttgtcg ggatcttgat catgatgttc
     9421 cgccaaaatg tagagttagc ttgggctaca attgcttcca ctccagtagc cgttttgata
     9481 gctgtcttga ttatccgcaa agcacggaaa tatgtggatc ttcagcaaga tgaagtgggg
     9541 aaactaaatg gttatatgga tgaaaaaatc agcggacaac gtgtcatcat cacaaatggc
     9601 ttacaagaag aaacgattga aggattctta gagcataatc aaagtgttcg cgaagcaacc
     9661 ttcaaaggac aagcttattc tggtctacta tttccgatga tgcaaggaat gtcactggtc
     9721 aatacagcga tagttatctt tttcggcggc tggttagcat taaatggtga tttggaacgt
     9781 tcggtggcat taggcttagt tgtgacattc gtccagtatt cacaacaata ttaccaacca
     9841 ctgatgcaga tttcatctgg atacagcatg atccaattag cgattacagg ggcaagacga
     9901 ttgaatgaaa tatttgatga aaaagatgaa gtcaaaccag aaaactgtaa agcatttgat
     9961 ggtgttgaaa aaggtcttgc gttgcagcac gtagattttg gttattctcc agaaaaacta
    10021 gtcttgaaag atgtctcgat caatgtggac aaaggagaaa tggttgctct tgttggtcca
    10081 acaggatcgg ggaaaacgac gatcatgaat ctgctaaacc gtttttacga tgtcaatgat
    10141 ggttcagtag tcatcgatgg aatagatatc cgagattatg atcttgatgc attacgtaga
    10201 catgtaggaa tcgttttgca agaatctgta ttgttctccg gaacgatcaa agacaatatc
    10261 acattcggca aaccggacgc ttcagatgaa gaagtcatca atgcagcaaa acaagcgaat
    10321 attcatgact tcatcatgac gttagaaaaa ggttatgata cagagatatc cgaagaaaat
    10381 aacttattca gtacaggtca aaagcagttg atcagtattg ctcgaacgat catcacaaat
    10441 ccatctttat tgattttgga tgaagcaaca agtaatgtag acacggtaac agaagcaagg
    10501 atccaaaaag caatggatga agcaatcaaa ggaagaacca gctttgtgat tgctcaccgg
    10561 ctgaaaacaa tcttgaatgc agatagaatc gttgtcttgc gtgatgggga agtcattgaa
    10621 gaaggcagtc atcacgagtt attagcagaa aatggatttt atgcggaact ttatcataat
    10681 caatttgtat ttgaataaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    10741 gccaatcaaa aacagcttct catgtttttg atttggttgg acttattacc gcagatataa
    10801 gcttttgagc accgtgaata aagaggttat gaaatcaacg ctctaacctg agtattagag
    10861 aaaaaaacgc aaaacatatc atttatgttt tgcgtttttt atttatcttt ttttctcaat
    10921 acaatataaa ataggcggct ggttcttttg gttgataaac cgataattca gcacattata
    10981 tgtttcctgt ggcagtttgg aacaaaaaga atcaaccata tccagttctt tttctccacc
    11041 ttcatgacca tagtagacga caaggatcaa tcgtccgcga ggcacaagtc gtttcaatat
    11101 ttcttccata gctgtgcgtg tagtctctgg catagtgatg attgctttat cactttttgg
    11161 gagataccct agattgaaaa tccctgcttt gagattttgt tgttcagcta tcacttctcc
    11221 taacgtttcg tggccttgta gaaacaatga gacacgttct tctaactgac gctgttctag
    11281 cctttcacga gtagcatcga ttgcttgttg ctggatatca aaactataga catgaccagt
    11341 ttttccgatg tgttctgcta gaaaaaccgt atcgtggcca ttgcccatcg ttgcatctac
    11401 gacgtgatcc cctggttgca agatttcttg cagtaatgta tggctgaaat gcaaagcagt
    11461 ttgcaacatc agacgctcac ctttttccgt acatcatatt ttccttggaa gctatctctg
    11521 cgaagaagtt cttcatcaat tgcattcaag acttcccatt ttttcaagct ccacattggt
    11581 ccaatcaagg aatcccacgg cgcatcccct gtcaaacgat ggatgatgat ctcttttggt
    11641 atcatttcca gctggtcgca aatgacattg acatagtcag gacggctcat taattgaagg
    11701 cgaccttcat gatagtcccg caacattctc gttttgcgca tcagatgcat caaatggagc
    11761 ttgattcctt ggatatctga atctaaaatg gtgcgtctga cattttcttt catcatttct
    11821 aaactttcgc ctggtaatcc gttgatcaaa tgcgtacaaa cacggatatt atgtttgcgt
    11881 aatttagcca cgccatctaa ataagtttga taatcatgtg ctcggttgat cagttttgaa
    11941 gtttcttcaa aagttgtttg caatccaagt tctacccata aatacatccg ctcgttcagt
    12001 tcagcaaggt aatcaactac ttcatctggc agacaatccg gtcgtgtacc gactgataat
    12061 cctacgacac ctggtaaatt gactacttgc tcaaatcgtt ctttgattat ttcaactggc
    12121 gcatgggtgt ttgtaaaatt ttggaaataa acgatgtatt gttggacatg aggccatttt
    12181 tgatgcatca tgtcgatttc tttctggaat tggattggaa gagggtcttc aggtgcaacg
    12241 atcatgtctc ccgaaccaga tacactgcaa aaagtacaac cgccatgggc aacagtccca
    12301 tctcgattcg gacagtcaaa tcctccatcg atcggcacct tgaaaatttt cccgccaaac
    12361 tcatttcgta aagcgtaatt ccaagaatga tatcttttat ttccttctga ataaggaaaa
    12421 gtcattaaaa gtccttcttt ctgtaagagt tatctatttg ttttcttttt tctctataca
    12481 ctggataagt caggcacagc caagaaagac tgagtaagta accgccgatg atatcagtag
    12541 gaaaatgaac gcccagataa atgcggctta tcccgacact tactataata aacatcatca
    12601 gcaattgaag caatgtgcgt aaggttcttt tttgaacaaa agctggaagc agaaatagta
    12661 aagaaccgta aagaatcata ctgcctgctg catgtccact agggaaactg taacttgttt
    12721 caactactaa gtgcgccaat gaaggacgct ctcgcatgaa aaatagtttg atcaccgggt
    12781 tgatgattcc agcaattaca ataaatccag aaacgaacca aaatgtttct gccctttttt
    12841 tcttatacca catccataaa gccgcacaag caaataaaat gatgacggta agaggattac
    12901 cgaatttagt cataaagagt tggtagctgt tcagatcagg ataagaagct ctgacaatgc
    12961 ctgtaaacca ctgatcgaaa ccagaaagcc acgacagatg gaactttacg atccagccca
    13021 aaatgaaaaa caggacaaga aagaagctac cagccacctg aaatgatgtt ttatatttca
    13081 tacaattcct cctttgaagc aatgacttat tatagcattt ttggtccagc gaaaaaaacg
    13141 gtaaagagat aaaaccaatg tatgattcct ctttgaataa ccaatattct tataaaaaaa
    13201 ataactgtaa tcggaaagac attttttggt aatgcaaatg aaatgttagg gaaataaaat
    13261 cagtgttttt cgaaatcctt ttaaacaaga acgtagacgt aggaagaact tgaaaatgaa
    13321 cagaagttgt tcattacggt aaattacaat atgattacaa aaaacaacaa agctcttaaa
    13381 taacttttaa taaatattaa gagaagttat aattattaat aatagataaa ttgaagttgg
    13441 aggatagaag atggaaaaca ttgcaagaaa agaacggaga cgtttagaac agaccaaacg
    13501 ttttcgaaaa gcaaaacgag gggcagcaat cgtgggaact gcgatggtag gctgttctgt
    13561 cgctgcccca ttagtacagc cggttcctgt tcaagcggat gaaacacctg ttcagttcgg
    13621 cgcaagaatc aatacagcgg catttatcac agaaattgct acttatgctc aaccaatcgc
    13681 tcaagccaac gatctatatg catcagtgat gatcgcacaa gcagttgtag aaagcggttg
    13741 gggcagtagt gcgttatctc aagcacctta ttataatctt tttgggataa aaggcagcta
    13801 tcaaggacaa accgtctaca tggatacgct tgaattttta aataatcaat gggtaaataa
    13861 aaaggagcct ttccgacaat atccttcttt tgctgaatct ttcaatgaca atgcctatgt
    13921 acttagaaat acttcatttg gcaacggtta ttactatgcc ggtacttgga agagcaacac
    13981 gaaatcctat acagatgcga cagcttgttt gacagggcgt tatgcgactg atcctggtta
    14041 cgcaggcaaa ttaaataata tcatcgcaac ttacggattg acccaatatg atacaccagc
    14101 tagcggaaac gcaggcggtg gagcgactac aggtggcaat aatactggaa gtgcaggtag
    14161 tactggcggt tcaagcaatt ctggtaatac aaactcaggt tcagcaactt cgaccactta
    14221 tactgtccaa tctggtgatt ctgtttgggg aatctcaaat aaattcggta tcacaatgga
    14281 tcaattgatc caatggaaca acatccaaaa caatttcatt tatccaggac aaaaactgac
    14341 aatcaaaggt agccaggaaa atggctcttc gacgaataat tccggtaaca ataccaattc
    14401 ttcaggaaat gctggtacat caaacggagg acaaacaact ggtgcgaaat acactgttca
    14461 atctggtgat tctgtttgga agatctcaaa tgatcacgga attacgatgg ctcaattgat
    14521 tgaatggaac aatatcaaga acaatttcgt ttatccagga caacagctaa tcgtatcaaa
    14581 tggcggttct tctaatgctg gagcaaatac tggctcaaat actgggaata caagtggctc
    14641 tagttcgtcc agctcaagtt cttctaatac agcttcagga acgaaatata cagttaaagc
    14701 cggagactct gtttggagcg tctcaaataa atacggaatt acgatggctc agctaatcca
    14761 atggaacaat atccaaaata actttatcta cccaggacaa caactgactg tttcaaacag
    14821 tggatcaaca aacagcacgt caggtaatac aggttcgtca accaatacaa ataacagcag
    14881 ttcagttaat actggcagca gttcgtctca aggcacttat accgtgaaag caggtgaatc
    14941 tgtttggagc gtggcaaata aaaacggcat cacgatggat cagttaatcg aatggaataa
    15001 tatcaaaaac aacttcattt atccaggaca gcaattgatc gttaaaggtg gaacttcggc
    15061 aaatacaaat acaggttcaa ctacttctgc agcgaaacca aatacaccga atacgtctgc
    15121 tacgacaagt acatcatcca ctggtaatac gatgtatacc gtgaaagcgg gcgagtcagt
    15181 ttggagcgta gcgaacaaac atcatatcac gatggatcag ttaatcgaat ggaataatat
    15241 caaaaataat ttcatctatc caggacagca agtgattgtg aagaaaggtt catctcaatc
    15301 aacaaaccaa caatcagtta ctggttcgaa aacctatact gtgaaatctg gagaatctgt
    15361 ttggagtgtt gctgacagtc atggaattac aatggctcaa ctaatcgaat ggaacaatat
    15421 taagaacaac tttatttatc caggtcaaac gttgattgta gctaaataaa atagctgttc
    15481 gaagaaataa cgattgaatt ccacaaaaat atttgttact ataaggctac ttaaatatga
    15541 caacacttga aaaggaagta gtaaaaagac tgaagtttca gagagcgcat ggctggtgag
    15601 aatgtgcacg gatgcttttg aactcgcctt tgagttgccc ttgctgaata cccgagtaag
    15661 caagcggcgg tgacggccgt tacaacgttc gaggcctttt cactaaggta aaattaggtg
    15721 gtaccgcgta gattacgtcc tataaaaaga gcaatctttt tataggattt ttttatttct
    15781 tttgtgaagt ttgagaaaac aaagcaaaag gaaataaaaa taaacagatt ctgccggttt
    15841 tttcgttata tttataatag aaacaagtgt tttcaataga acaggaggac agcaaatgag
    15901 ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac tgggcaaaaa cgaatacttt
    15961 caacacccat gatgatcctg aaaagccaaa attttacgca ctggatatgt ttccctatcc
    16021 atccggtcaa gggctacatg tagggcatcc tgaaggttat acggcaacgg atatcctttc
    16081 acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca atggggtggg atgcattcgg
    16141 tctgcctgct gaacaatatg cgctagatac aggaaacgat ccagctgagt ttacccaaaa
    16201 aaatatcgaa acattccgac gccaaatcaa ttctttagga tttagttatg actggaatcg
    16261 cgaagtgaat acaactgatc cggagtatta taaatggaca caatggatct ttacgaagct
    16321 ttatgaaaaa ggtctagctt atgaagcaga agtagcggta aactgggtac ctgaattagg
    16381 aacagttatt tcaaatgaag aagtgatcga tgggaaaagt gaacgtggtg gctatgatgt
    16441 agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact gcttatgctg atcgcttatt
    16501 ggatgatcta gaattagttg attggccaga aagcattaaa gaaatgcaaa gaaattggat
    16561 cggtcgttca gtaggagcaa atattgaatt taaagtggct ggtacggata aatcctatac
    16621 cgtatttacg acacgaccag atactctatt cggtgcaact tactcggttc ttgcacctga
    16681 actagatctt gttagagaaa ttacgacccc tgaacaaaaa gctgctgtag aagcttatat
    16741 cgaagagaca gctaaaaaat cagatctaaa gagaacggat cttgcaaaag aaaaaacagg
    16801 agtatttact ggtgcttatg caatcaatcc agttaatggc aaggaaatcc caatctggat
    16861 cgccgattat gtattatctt catacggcac aggagccatc atggctgttc cagcacatga
    16921 tgaacgtgac tttgaattcg ctcaaacttt cggactagaa attcttccag ttatcgaagg
    16981 tggagatgtt gaaaaagctg cttacacaga agatggtacg cacatcaact ctgaatttct
    17041 agacggcatg aacaaacaag aagcaatcga taaaatgaat gaatggctag aagaaaatgg
    17101 cgtagggaaa aaagaagtca gctatcgctt acgtgactgg ctattctccc gtcaacgtta
    17161 ctggggtgaa ccaatcccaa tcatccattg ggaggacgga acagtcacag ctgtcccaga
    17221 agaagagcta ccattacggt tgcctaagac aaaaaacatc aagccaagcg gtactggaga
    17281 atctccatta gcaaatatcg aggagtgggt caatgtcgtt gatcctgtaa caggtaaaaa
    17341 aggccgtcgc gaaacgaata caatgccaca atgggcagga agctcatggt attacctgcg
    17401 ttatatcgac cctcataaca aaaaagaact agcagattat gaaaagttga agcgttggct
    17461 accagttgac atctacattg gtggagcaga acatgcggta cttcatttac tatatgcacg
    17521 attctggcat aaattcttat acgatatcgg tgttgttcct acaaaagaac cattccaaaa
    17581 actatacaat caaggaatga tcttaggcga aaacaacgaa aaaatgtcaa aatctcgtgg
    17641 taatgtagtc aatcctgatg atgtggtcga aacttacggt gcagatacac tgcgcatgta
    17701 cgaaatgttc atggggccat tagatgcttc gattgcttgg agcgaaaatg gcttagaagg
    17761 aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt gatgagaaca ataaaatgcg
    17821 tgaccgtatc actacattga atgatggcaa gcttgataaa gtgtatcatc aaacagtgaa
    17881 aaaagtgaca gaagactatg aaaatcttca tttcaataca gcaatctccc aattgatggt
    17941 ctttatcaat gaagcctata aagtggacgc tctgccttat gaatatatcg aaggatttgt
    18001 tcaactactc gcaccgattg ctcctcacat gggtgaagaa ctatgggcaa tccttggaaa
    18061 tgatggtggt atttcttacg caccatggcc aacatacgat gaagcagcac ttgttgaaga
    18121 cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt gcgaaaagca atgttccccg
    18181 agacttagga aaagatgagc tagaaaaagc agcattagcc aatgaaatag tgcaagaata
    18241 tattgaaggc aaaacagtca gaaaagttat tgcggtacca aacaagttag tcaatatcgt
    18301 tgctaattaa ttggctcttt gtcaataagg actgatgagt tgtgcaaaat caaatctggg
    18361 ccagaatgaa cttcattctg tctcagattt tttgttattt caaattgatt aattgattga
    18421 tcaaaaaggt tttgattttc atgttctcaa acacgttcaa tgtctttttc ttctgaatgg
    18481 tcttgtaaga gatctctgta atcatcaaat ttttgtctat taatcttagt attttttgat
    18541 gtataaacta cctgaaattg agagtacata ccaaattcat aaatttttta ttagtgggac
    18601 tagtgattgt atggattttg acgcataatg aatgaagtat gagaaaagga gagacaaatg
    18661 gatcaagaac tatttattat cgaagtatta tcagctaaac aaaaaattat cgagaaagcg
    18721 aaaaaaagag cgaaaagaat aatgttcaag cttttaagct tagtattttt ctctggtttg
    18781 ctttttttcg gagctgtatc gttcctatat cgccatatga gtgcagtagt ccgttcagct
    18841 gaagggatgc aaaaattaaa taatttgtat cacttttcgt ggggagttgg cggaataaca
    18901 attgttgtgc ttattgtttt tcttctactt atttggctcc gttaccatca attgtcgcgt
    18961 catttcgatc aatggcttag tcaaagagca caacagtttt tacataataa accaacttct
    19021 caagatgatg actatcttta ttttgagaag acttcgacga gtcaagcttt tcggatacaa
    19081 aaaagtgatt gccagttatt gctgagatca cttgatgatt tacctatcta cttaggcgaa
    19141 agcaaatcaa aattcggaat gaacacattc catatttttt tactagaaaa cgaacccaga
    19201 tgggaaaaaa gccagtcttc gcctggtgga tacctgacaa ctaagatgag tacattagtc
    19261 actggttttt tcctcattct tttagctgga acaatctatt atgggagata cactcagcca
    19321 aaaatgtctt ctcatataaa tgctgcttct cacatgacaa tgaactcaac taacgatacg
    19381 catatacaaa agacaaatga agggttgtta gctgatccta agcaaaaggt aaatttgaca
    19441 acacaaaaga taaatgatct caaacttgat acagagacgc actctctcta tatgacaaca
    19501 gatagtggtg ctaattgggt atttgtgccg attgattcgc aatggttgcg ttttggcgaa
    19561 tacaccctga caacggggct gattcctgaa ggttattgga tggacaacac atatgatatt
    19621 tccgccgatt tctcatggtt tatctattct tctggggatg atgtacgtct gctgacttca
    19681 agagataatg gtaaaacatg gcaaacgtct attatcaccc aatcagttca gccgatacgt
    19741 tatcgaaaag cggtatttta tggcaacgat ggtctattta tctgtacatc agccagtggg
    19801 atgtctgcag aaactttgta catttacact actatggatg gcggaatcac ttggataaaa
    19861 agtggaagta caatgatcaa tcagccagta caaaatgtca gcttcgttac aacttcttta
    19921 ggttttgttt cgacacgtga aagtttgttt tatacaaaca acggaggacg ctcattcaaa
    19981 gaatcagttc tgtcgattcc ggaaaactat cagacagggg ggatagatct ttttcaatca
    20041 ccaaatgaag ttactcaaat aagcgcgaat acgttagaga caaaatttta tttgttgaaa
    20101 aaaggaccaa tcgatgcagg gaaaatgttt gcctgcctct tccgttcaac tgataacggg
    20161 gaaacatggc aattcgttca gcaactatcg caagtagaac aaggagatta aagaagagag
    20221 attgtgagcc tgacgttcag caccgagtca caagaaacaa attcaaaaaa cagcttctca
    20281 tatttgttga atagtgtaac tcgtgattcg gagttgacaa gagcaacaag aagcgatttt
    20341 gaagtatagc ttctcgtatt ttacaagatc gtgtcttatt gccgataggt tgtctcttga
    20401 acaccgttta ttcgtaaaaa gggattgtga caagtctttt gtcactactt accgaacgaa
    20461 actgaaggag ctatccaaaa agtatcatta gtattattct ttgtgtttct agtggcacca
    20521 ctagtacatt taaaaaagct atcgatcatg gactaatcat acatgatcga tagcttttta
    20581 gataatgttt gttacgctat ctgaaattga ctattataaa gactggcata aagcgttggt
    20641 gattggagta gttcagaatg tgttcctttt tcgacgatat cgccattctt cattacaagg
    20701 atcatgtctg cattttcaat cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct
    20761 tttgtcacag cttccattgc ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa
    20821 gttgcttcat ctaaaataac gacaggagga ttcgctagaa tgactcgagc aattgtcaat
    20881 aattgttgct gtccttgaga taacgtgccg ttttcgctag aaatgatcgt atcatatccg
    20941 tctggcagtg ttcgaataaa gtgatcacat tgtgcgattt cagccgcttc gataatttgt
    21001 tctcttgtgg catccatttt cccgtaggcg atattatcag ctaccgttcc ttcaaacagc
    21061 caggtatttt gtaaaaccat cccaaaagaa cgacgtagtt ctccacgtga aagcttggta
    21121 atatcaagac cgtcgaattc gatctttcct ttattcagtt cataaaaacg catcagcaga
    21181 ttgactaaag tcgtttttcc tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc
    21241 tttactgaaa aatcgacttg attcatcaat attttatctg atgtgtaacc aaactgtaca
    21301 tttttgaaag ctatttttcc tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga
    21361 atttcatctg tttcatctaa tatctcaaat atcctatcga ttgctgccat tgcggattgg
    21421 atcgagttga tcacataaga agcggtagaa attggttcag aaatctgatt gatgtactga
    21481 agataagctt gtaagaagcc gatcgtcaca ccacctgata gaacaagcat agcaccgaat
    21541 accgcactta tgatgaaggc gagttgatta atgaaacgaa tggccggata gatagcacag
    21601 ttcataaatt gagcacgttt aaaagcgttg tattgctttt tatttacttc gtgtatagca
    21661 gcttctgcat gagcttgctg attgaatgtt ttgaccacta gattaccagc taagatttct
    21721 tccattcgat tatttaaaac acccagttca tactgattct gttcagaaat ctgtttgttt
    21781 ttgttcgcaa tcttagttgt aacaattgaa ctgccaacga tcagcaataa gacgagcaaa
    21841 gtaagcttgc tttcgatata aaacagcatc agaccagcag cagctattgt tacgacagaa
    21901 gtaaaaaatt gattgatacc tgtcaataac acttgagaaa gttgattgag accggtagtt
    21961 gtccgactaa tgatgtcacc tacttgatga ttgtcaaaaa acgacatcgg taatgttttg
    22021 aatttttttg tgatttcttt tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt
    22081 gtccgttcct gaataaatga agtgatgctg cttaagactg aacagccgat cagcaataag
    22141 acagggaaca acaatgcttg tttgatatca ctgaaagaaa gctgtccaat acctgttttt
    22201 cttatttctg ataagagatc atcgatcccg attcccatga tcaagggcat ggctacgact
    22261 aataggttcc cgaataaact gcatagaaac aatccgtaaa aatatttatg ttcaggtttg
    22321 atcattttcc aaaaacgtat caaagcacgc gtgttcaacg tttttttctt ttccatcgta
    22381 tcccttcttt ctatattcct tgtgattttg caaactcctg ataagtttgg caattagaca
    22441 gtagttcttc atgagtacct tgtccaacga tttttccttc atctaaaaca atgatgttat
    22501 ctgcttgttg gattgtactg aggcgttgag caacaatcac gagagttttc tccgacatgt
    22561 gtttatgaag ggctgctctt aatgctgcat ctgttttata gtctaaagcg gagaaactat
    22621 catcgaatac atagatctct gctggtttga tcaacgctcg agcaatacat atccgctgtt
    22681 tctggccccc tgaataatta gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa
    22741 gggtgtccac tagttcttct aattgagcga tcttgatcgc tttagacaac tcttctgttg
    22801 aggcatattc atttcccatc tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct
    22861 tctgagggac ataactgatg tgggagcgga tctgttcttg agtcatttga gtaagaggaa
    22921 taccaccaag ggttactttt cctatcgtag ggtcactaag tctcaagaga agtttcgcca
    22981 aactactttt tccagatccg gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg
    23041 tgaaatcaat atcttctaga actggatcag agatttcagt atatttaaat gtgacatgtt
    23101 caaaagcagc tgcatttgtc tgatcaaagg ttggttcagc tactgttttg atcgtctctt
    23161 cctcaatttc gatctctgct tgcagtattt cttctatccg tcgtaatgag gcaaacgatt
    23221 tagggataat gaccaatacc atagctgcga tcatcaagta agcaagtgtc agcatagagt
    23281 attcgactac agcagtcacg acaccgattt gtaatgctcc ttctgctaca agatttccgc
    23341 caaaccacaa aatagaagca taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc
    23401 aagcaaatat tcggttcact ttgatcattt gattagcata gtcagcaaag ctcttattcg
    23461 ttcgcttttc ttcatatgat tgattatcaa aggcacggat catattaatc ccagtaaaga
    23521 attctctcaa tgtgatggtg atccgatcca tttttggctg gatactcaaa gataaaggtg
    23581 cccctcgttt catcagtcga taaacaatcc atacataaaa aacaacagag atcaatgtaa
    23641 cccatgttag taatagagaa taacgaaaca tcatgagaat ggtgaaaacg caaataaccg
    23701 gtgctggtaa gatcatttgg caaaataaaa caatcatttg ctggacatta tcgacatcat
    23761 tcgtcattct tgtcaaaaga gatccagaac taaaacgact gatatccttc attgacagct
    23821 gttggaattt acggaaatat ttctcccgcg tttggaatcc aaacctagcg gaagccaatg
    23881 ctgaaaagta actagaggca actgcagcga ttgttcctag aaacgctaca agcagcattt
    23941 caactccaat tttttttata actgaggctt cgccacttgc tatacctgtg tcgatcagct
    24001 ttgctactaa ttttggtaca cctaatgtac cggcgatttc taaacagatc atcacaaagg
    24061 taaggagagc gagtcctttg tttttctgta agaatgtttt taagatagac atattattcc
    24121 tccttttttc ctgatacttt tcgattctat accttcaagt aacttgaagg tcaatagaaa
    24181 aaagaataaa ctgaaaaaga ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt
    24241 atcggaacta tttgatttgc caaaatcaac gatccgctat tgggatgaac aaggattaat
    24301 ccgttcaagt agacaagaag agaatgatta tcgattgttt gatatcgatg atatttttat
    24361 gctttatgat atcgtttttt atagaaaatt agatataccg atcaaacaga tgaagaattt
    24421 atatggaaaa acattgacgg agttatatga gacacttgat gaaacagaaa gaagaatcca
    24481 taaagaactg gtggtgatga aacaaaaaca aaaagagata agggagcaaa aaaaacagtt
    24541 gaaattgatg atcgatacaa acgaggaaga atttcctgta gaagagatcc cttttgactg
    24601 tatgatctca acggagtttg aagatattgt ggaaatcaaa aaatttctac ctaactattc
    24661 aagctttggg atgatgagta tgccagcttc cagttcacag acgatgtatg gattttttat
    24721 cgaccctagt gaagtacatt tatttactca agacgtgatt tgggagaaaa aagatacggc
    24781 tgtttatcgt cgattcttat tgaaatccga gatgaaccat gcggaacgta ataatatttt
    24841 agaaatcaga gaaagaatgt atgaaaaagg atggaaaaca ggagaagtga tcggccagta
    24901 tctattgacg aatacggatg aaaacaatat cagaactgaa tattatcatg catggataga
    24961 aatgaaaaaa taggaaatat gtgtttaaga taactcgaaa atgatggttg ttttagttat
    25021 aaagaaaaat tttctggttc gattacgggt ttaatgtggg ggattattag tcgtggatag
    25081 atagaatttt agttttttag aggaaatatg tctttaaaaa tggaatggtt gcaattctta
    25141 gcgatatata cgatgtaatt gtcctcttaa aaaagtatgg aaaaataatt gtattgggtg
    25201 ttatgtataa ttcatggtga actgagacta atgagaagaa aaattcatat tgttatgagt
    25261 gagattgtat cagatgagaa tgataaatta aataggtgca taaaagcatt acacttaaaa
    25321 tacattgttg cttacattat tacaatatgt taataaggca aaaataaata gatggattga
    25381 ataaacatat tttttaatca aatagtccct actgtgactt tcctcagcta tcggtgatac
    25441 taccgatagc atttttttgc ttataaaata attataagta tttagagatt tttcttctaa
    25501 cattcttcaa acaagaaaaa tcacaaaggt
//
LOCUS       DANJJH010000033        25501 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000033 DANJJH010000000
VERSION     DANJJH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            133..3660
                     /locus_tag="PWS53_001302"
     CDS             133..3660
                     /locus_tag="PWS53_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL0871535.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="PWS53_001303"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="PWS53_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL0871536.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="PWS53_001304"
     CDS             7448..7789
                     /locus_tag="PWS53_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0871537.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="PWS53_001305"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="PWS53_001305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL0871538.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="PWS53_001306"
     CDS             9146..11566
                     /locus_tag="PWS53_001306"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL0871539.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWS53_001307"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="PWS53_001307"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL0871540.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="PWS53_001308"
     CDS             13000..13737
                     /locus_tag="PWS53_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0871541.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="PWS53_001309"
     CDS             13751..14566
                     /locus_tag="PWS53_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0871542.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="PWS53_001310"
     CDS             14579..15226
                     /locus_tag="PWS53_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0871543.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="PWS53_001311"
     CDS             15242..15898
                     /locus_tag="PWS53_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0871544.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="PWS53_001312"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="PWS53_001312"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL0871545.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="PWS53_001313"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="PWS53_001313"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL0871546.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="PWS53_001314"
     CDS             18240..18758
                     /locus_tag="PWS53_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0871547.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="PWS53_001315"
     CDS             18865..19365
                     /locus_tag="PWS53_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0871548.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="PWS53_001316"
     CDS             complement(19645..20565)
                     /locus_tag="PWS53_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL0871549.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="PWS53_001317"
     CDS             20730..21743
                     /locus_tag="PWS53_001317"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL0871550.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWS53_001318"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="PWS53_001318"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL0871551.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="PWS53_001319"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="PWS53_001319"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0871552.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="PWS53_001320"
     CDS             complement(23383..24144)
                     /locus_tag="PWS53_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0871553.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="PWS53_001321"
     CDS             24333..25427
                     /locus_tag="PWS53_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0871554.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 g
//
LOCUS       DANJJH010000034        24579 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000034 DANJJH010000000
VERSION     DANJJH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1778..2443)
                     /locus_tag="PWS53_001322"
     CDS             complement(1778..2443)
                     /locus_tag="PWS53_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871555.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2623..4605
                     /locus_tag="PWS53_001323"
     CDS             2623..4605
                     /locus_tag="PWS53_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL0871556.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="PWS53_001324"
     CDS             4609..5247
                     /locus_tag="PWS53_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871557.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="PWS53_001325"
     CDS             5275..5703
                     /locus_tag="PWS53_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871558.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..7071
                     /locus_tag="PWS53_001326"
     CDS             6160..7071
                     /locus_tag="PWS53_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0871559.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            7288..7725
                     /locus_tag="PWS53_001327"
     CDS             7288..7725
                     /locus_tag="PWS53_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871560.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7820..8704
                     /locus_tag="PWS53_001328"
     CDS             7820..8704
                     /locus_tag="PWS53_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0871561.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8928..12095
                     /locus_tag="PWS53_001329"
     CDS             8928..12095
                     /locus_tag="PWS53_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871562.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            12115..12660
                     /locus_tag="PWS53_001330"
     CDS             12115..12660
                     /locus_tag="PWS53_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HDL0871563.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12688..13143
                     /locus_tag="PWS53_001331"
     CDS             12688..13143
                     /locus_tag="PWS53_001331"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HDL0871564.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            13140..13898
                     /locus_tag="PWS53_001332"
     CDS             13140..13898
                     /locus_tag="PWS53_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0871565.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(13984..14724)
                     /locus_tag="PWS53_001333"
     CDS             complement(13984..14724)
                     /locus_tag="PWS53_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871566.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            14785..16038
                     /locus_tag="PWS53_001334"
     CDS             14785..16038
                     /locus_tag="PWS53_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0871567.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16360..16587
                     /locus_tag="PWS53_001335"
     CDS             16360..16587
                     /locus_tag="PWS53_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0871568.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            16710..17036
                     /locus_tag="PWS53_001336"
     CDS             16710..17036
                     /locus_tag="PWS53_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0871569.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            17104..17817
                     /locus_tag="PWS53_001337"
     CDS             17104..17817
                     /locus_tag="PWS53_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL0871570.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            18127..18978
                     /locus_tag="PWS53_001338"
     CDS             18127..18978
                     /locus_tag="PWS53_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0871571.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            19099..20964
                     /locus_tag="PWS53_001339"
     CDS             19099..20964
                     /locus_tag="PWS53_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0871572.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            21012..22421
                     /locus_tag="PWS53_001340"
     CDS             21012..22421
                     /locus_tag="PWS53_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL0871573.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            22529..23230
                     /locus_tag="PWS53_001341"
     CDS             22529..23230
                     /locus_tag="PWS53_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0871574.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            23389..23868
                     /locus_tag="PWS53_001342"
                     /pseudo
     CDS             23389..23868
                     /locus_tag="PWS53_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         8280 a         3995 c         5045 g         7259 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     7081 ataaaaaata tgaactattt aaatcaataa aactgttcat ctaaatcttt ttattagaac
     7141 taaatcaaca gtagtttaat tactacttga tttagttctt tttattttaa aaattttttt
     7201 tacttagaat ccagcgaccc tagatatttt tctgtataat cttaaataaa gaaaacaata
     7261 tcaaaaaatg cacgtagaag tagataaatg gagtggtttt gcaaatttct tttatttcta
     7321 gaagttattc acgtatacgg atttgttttt ctcacaaata aaaaggcagg aagtacaatg
     7381 gatcatttgt atataaaata tattcatcaa ttttccgttt tggtgttagg cacactaatt
     7441 atagcgggga ttgtcgcatt tattattcaa ggccgtgaca tattaacagt acatcaagag
     7501 tttttcagtt atgcatttgg tattcctctg atcgctataa ggtctgataa caaaaagtgg
     7561 aagaaacgct attacattct cttgctattt ttgtgttttt atctgccacc taagattttt
     7621 actttattag gtatacaatg cctatcattt tcttttattt cctatatcat tgttttaatt
     7681 ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa aataaatgaa tgtttcatga
     7741 acagtacttc atcacagcct aatttttttc tttatgctat cgttatatta aagaaatcaa
     7801 tcagaaggga aagaaagtga tgaaaaaagt tttattagtg gtaaacccca gttcaggcgg
     7861 tgaacaagca aaggaatttg agcaattagc gatagcaaaa ttggaatcgg tgtttgatga
     7921 agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa aactttactc gcgaagcggc
     7981 tacggaaggg tatcatagtg tgtttgtcat gggcggagat ggaacagtca acgaaggaat
     8041 cagtggaatc gctgaacaag aacatcggcc aaatttcgga tttttcccat tgggtacggt
     8101 gaatgacttg gctcgagcat tagggatacc gctagaacct gaggaagcga tcaatcattt
     8161 ttcgattgaa tcagtcaagc cattggatat tggaaaaata aatgacgact attttatgaa
     8221 tgtcgtcgca attggctcta tccctgaatc aatcaatgat gtagatgcgg agaagaagac
     8281 gaaatttgga aaactagctt atttcatgtc aggaatcaaa cagttagctt ccacacaatc
     8341 ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa attgagagca gtaccttgct
     8401 gataggacta actaactcag tcggcggttt tgaaacatta cttccaaatg caaaagtgga
     8461 tgatggtaag ctccatttgg tttatttaaa agattcttct ttgatcgata cgcttaaaac
     8521 attgccagac ctattaaaag gagtagatga atctacgaaa aatctagcct accttacttg
     8581 tgagcaatta gttgtgtcat tagctgatga ggaagaattg acgacgaatg ttgatggaga
     8641 tgaaggggat aaattacctg tgacaatcaa agtattgccc tcacatttac aggtttattg
     8701 ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca ccgcttgttc gggataaggg
     8761 actgtgacaa gacttttgtc acagcctttt ttttgtacaa aaaagaaatt tacacctaat
     8821 agaaaaattt ttccataaaa atacatattt taaatatgaa agtacaattg gaaatagatt
     8881 aaaacgataa aataaaattg tcaaaacgtc agagaggatg gtggagaatg aatcaatgtg
     8941 taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg acatccgttt
     9001 ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga gaatcaaagt tttgacaaga
     9061 ctactttttc agaagaatcg attacttctg aaactacaga atctcaaagt gaggcttcta
     9121 ctaataaaag gcaagaagaa aataagacaa gtgagacctc agaagtagct gaacaaacgt
     9181 ctgatagcag tgaagcggca ctggcaagag ctgtttcagc ggatgggtac agcgaagctg
     9241 ccacaacaga agaactggcg cagcttctcg cagacgaatc agtaactaag atccgactga
     9301 ttcagccttt gactttagac agggaattag agatcaaacg agatatcgtt attgattttg
     9361 gtggattcgc tcacaatttc ggcacacacc atatttatat caatgagata cctcagtcga
     9421 tcgagttcca gaattttaaa ggaacagctg cacatccagg tggattgaca ccaaatatcg
     9481 atggaaatgc cattatcatt gcttatatga ccgatttttg gggaagcaga tatcatttta
     9541 cgggagaaat aaaatttacc gggacacttg atttgtatga tgggtccaaa ttaggactga
     9601 tttacgctcc tcgtgcaact gtcacattag atggtgtttc cggtgtgttg gatgtccaac
     9661 cagtgaagga aggactgaat gccgctccgg gtaaagccta tttttgtaga agctatcaat
     9721 tgaatgtgat caatggtagt caactttatg gaccgtatct agggaaattc tacggttttc
     9781 tagattcagg agatgagact ggtagtggaa aaacagcgcc aggaatccat atcttaagcg
     9841 gttcaaaggt ctctcttgat tatgatcggg cagatggcgt tagcgatgaa ggagaggcag
     9901 tcgatacgct acctggtaat gtcatgtttg aagtcagtgg ttcgggaagc gaattttcgg
     9961 taaatacaaa gatcaatatt accaatgaca acaatcgcgg aatcatccaa atgagaggtt
    10021 caggatcgaa agtcactgta tcgaatggtg ggaaaatcac catcaataca gcaactacgg
    10081 gcggatttcg cttgcaagga gattctagtc aaatccatgt atcaagtggt ggacaaatct
    10141 cggtcaaaat ggctggagat gacgatcaac ccgggaacaa tgggatgcgt tttgtaggaa
    10201 aaggcctttc tttgatcgta gaaggagccg gatcagttat cgacatcgat aagcagagtg
    10261 ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa gctgacagta accaatggag
    10321 gctcattgag ggtaagaaat acaggtgatg gcaaatctta cgtgaacaga ctcaatcaag
    10381 gaaatcaagc gatccaattt tatgacgctt ctggttttct agaaagtcca ggttcagctg
    10441 attttacagt cactggagaa aaatcaaata tcgatatacg cgcagattcc ggagcaactg
    10501 tagatacaac tggagatatt gatttaaatt ttacggcagg tgaaaaaact tatttagtca
    10561 tggtcgggaa aacaggggaa gataagaccg gcatcatcag tggggatgtg ttgaatgtgg
    10621 acttagaatc tcctacctat tttgatttcc aaaacaaacg gattggaagc gagaccaatg
    10681 gcataggcgg ctgggtattc caaggaaatg gagattcttc tttaaagatc gcccattcag
    10741 aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa ctttgaaaaa gacccggatt
    10801 atttttctga tatgacagac ttatcgatca atgggaccga cttaggcaat ttcgtatcaa
    10861 gccctagtgc aggtttaaca aatgaatttg ctcagacagg tgccggaatg aaaaattata
    10921 atcgaatcag tgccaataat cagctcccga tgattgaatc cttaagagtg ccaacgaatg
    10981 cagataaaaa aatctatggg catgtcgttg ttcccgaagg tgtcgaggca aagccaagag
    11041 atgcttggac aggcgaagta gaagtgactt tacgtattac ttacgcagat acgacgaaat
    11101 catctgtaga aattactgct ctcacgcaag gacaaacaga tgaaaatgaa ggggcaggat
    11161 ataacccatg gggagaaggc gaacaaggcg gcttgttcga agttcaagta cccaatgatg
    11221 agagattaaa aacggaggat caagtgcaag ttgttgctgc taaaaaagta agaggtggcc
    11281 aagaagtgac agaactggac tctgccaaaa cgacagtcga tgtgattcct cctgaacctg
    11341 ctaatttaag taccacattg atcaatgcag cttctcgcac gatcacaggt acaggtgaag
    11401 caggagcctt ggtttcattg aaaaaaaatc aagctgtttt gtcagaaaca actattgatg
    11461 atacagggga atttgttctt tctatcccag aaaatcaact ttttgcagga gatgtattag
    11521 aaattgtatt aaacgaccaa gcaggagaag ctactgctaa gggtgtgcag aacaagccaa
    11581 gtaccaacga tgaaattggg aatcacaatc caagtgatac gccagttgtt tatcatgatg
    11641 ccccgccttt tgcacctgct acgaagatcg tagtagaagg tggtctctca tttcttgccc
    11701 cttccaaact cgattttggt cagatcaaag caactggttt gacacaagaa gcatttggcc
    11761 aaatggatat acaggaaaaa ttatggattt atgatcagcg agaaacgaaa aatccgtgga
    11821 tcttaacgat aaaattatct cagcctttca caaatgatgc aggattttct atgcaggatg
    11881 tcctatattt caagaaagga gaggagtatc aattagtgaa tgaagaagaa ctggaaattg
    11941 tcagaggaca aaattcaacg aatgtgcaag cagattattc agaatattta aataacggtc
    12001 gggcgtttaa acttgtctta accaaagaaa atcagatagc tggtaattac tcagcagaga
    12061 taacatggac attagcagat gcgccgacag aataaagatc aaggaggatg attgatgaat
    12121 cgaatatgga ataaaagtca tcgaaacaac tgctctttac ttatgatcct tctccttgga
    12181 ttcatccttt ggcagacacc tgtccatgcc tccgaattca attttgcggt agattttgaa
    12241 ttaccggcta atcagattga taagaacaaa acatatataa atgtaaggat ggagcctggg
    12301 aaaacacagg aaatccccta cacggtcttt aaccgaacag ataaatcagt aacagttgag
    12361 acattgatcc actccgctac gacgaattca aacggtgtga tcgaatatgg agaatcaaaa
    12421 agtaaatttg atgaaacatt aagttatgat atgaaaaact tagcaaaaac agtgggagaa
    12481 ataactgttc ctcctcaaag tgcggtcact gagacacttt cactgaaagc gccagaaaaa
    12541 gagctggctg gtgtggtcgc aggagggatc acgtttcaag aaaaaaaaga gaacgtttct
    12601 aacaaaagta cagttggtgt cgtcaatcag ttttctcaag tgaaagcaat cttgatttga
    12661 aatagcgaaa aagagtcttc cccagatttg aaattaaaaa aagcttatgc ggatcaaagc
    12721 gatggaagaa atgtgattaa ggcgaatctt caaaatccaa atgcttctta tatgttcggc
    12781 ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag agccttacct ttcaataaaa
    12841 aaagaaaaaa tagatgtagc acccaataca aattttgatt tacttgtccc attagaagga
    12901 aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg gtgcttggaa agaaaaacgc
    12961 tggaatctcc ataccgattt tgaaataact gatgatgcag cggaccaatt gaatgaaaag
    13021 gatattgatt taaaaaatca gccagaaaca tttgactggc gatggctgat acttgttgga
    13081 gtcattctta ttggcttatt tgctggatta tttattttaa aagaaagaag gaaaaagaaa
    13141 tgaaaaaaac aattgcatta agtaccgttc tagctggaag tctattatta tgtacacaaa
    13201 gtgtttttgc tgagaataag agctcagcca ctacagaagg aacgatttct gtgaccaaac
    13261 cagatgctgc aaatcctgaa gacgttgttc cagttcctcc tgttgatcca aatgatcctg
    13321 atactccttt tacacctgac gaaccaaatc agggaacatc cggattactg acgattgacc
    13381 aagctccaga tcttgatttt ggaacaatca agttaggtac agcaaaaaca gtatatgctg
    13441 cattacaaag cggaacggac agtactggag cacaaacaca agtacagaat ttcgtacaag
    13501 taacggataa aagcggaaat tatgccggtt ggaaattatc agtcaaacga acagaattta
    13561 ttggtacaac atctgatacg aataaactaa ctggttcaaa ggtgaaattc aaaaacgccg
    13621 tgattcgaaa tagcgcaact aatagcagtg gaatgccatc taccgtttct ggaactggcg
    13681 ctgaaggaat cgaaatccca gcagatcagg cagtagatct agtaactgca ggagcaaatg
    13741 aagggattgg cacatggatt tattcattag gagagaatgt ggaacaaggg agacaatcag
    13801 tggaattatt tgttcctgta tccaattatg ctgtagacaa ctacgtttca acattcacat
    13861 ggtcgttaac agatgctcct acagaagagg aagcgtaaaa tcatatcaac gatttcaggg
    13921 agtggagccg attcaaggcc cactccctga aatctgctat gcttacgtct cagctcttat
    13981 tcatcaactc gaagcagttt tttggaagtc atggaaacgt tcctttgtct tttcaaagat
    14041 ctttcgacca acgagtaagg agtcaccgtt gaaaaattct aacacataat gagcagaatc
    14101 aattttctgc aattgcgata agttgataca gtaagatttt aaaagtaaaa agtaagcata
    14161 cttttctaac tctgtcttca attgttttaa agaagtattc actaatttat cttctgtagt
    14221 tgttttgacc ataacttggt ttttcaaacc tttgatactt tcaatatata aaatttcccg
    14281 aggatacagg acgagacttc cacctttttg atcaataaag attttatcga catttacgat
    14341 ctgctgttct ttccaattga tctgttctag cacttgacga atttgttcag ataaattcct
    14401 gtcttttctg atatatccaa tcggaaggat tttccgattg atgatctcta ctgcaaaatt
    14461 cgtataagcg gtgaaaaaaa caatattact atgtgcattt tttttacgta tttgttcggc
    14521 gacatcaata cctgtgagtg attgcttcaa gtcgatatcg ataaagaaaa catcgtgatt
    14581 atcaatagtg cccaactgga tcaactgatt gatttgagag atagaagtga gctgcacgat
    14641 attctcgata tgatgattga ccaattctct gatgatccat tcgctgtgaa actggttatc
    14701 ttctatgata aaatactttt tcatatgcag gctcctatta aaaaaatatg tgtacatttt
    14761 gaaacaaaac aatggaggaa taagatggtc gcaacagtga cactttattt gacctttact
    14821 ttgattttag atttaggtat ctatttattc ttttcgaaac aactgctagt aaaaaacagc
    14881 tttggttgta tagctttgat ggtggtgaca cttttacttc ctatccctaa agtgagcttt
    14941 ccttacctag ttggcttatt attgatgttt cttttttatt ttactacgaa aaacctgttt
    15001 atcagctgtt tgatcagtaa tctttttttt gctatcactg gactcagctt ttttctggtt
    15061 tttgatctga tggattattt aggaaagaga catcttggaa cagcaagttt ttttgcacta
    15121 cttatcgcat ttattttcta tctattagta aaaagtatag acaaacagac gagatttctc
    15181 aacttgcttt ttacttatcc aactgaacac tattttggag gtatttgtat tttattaagc
    15241 tggggcgtga tttacttggt attgggaagt gttcacttcc gctcatctaa ttatctgctt
    15301 ctatctcttt tgtcgatagt catcagtttt ttttctttgt ttttttccat tatgttgatc
    15361 agtttccaag taaaagaaag acaacatcag gaatcgcttc aactgtatca aataaatgaa
    15421 gaatattacc atcatctaga ggaattcaaa catgattata aaagtctact gttttcatta
    15481 aaaattactc ttcaggaaaa taaaaaagaa acgttggaat ttttagaaat attagaagat
    15541 cattctgcag ctatttttga aaaaccgcaa atacaacagc taacaaatat tgtatcccca
    15601 gctgtcaggg gtgtcttttt aaaatttcta gaaaaagcag aatcggagaa aatcccgatc
    15661 caagtagtta ttcctgtcga agtgaaaaca atcccgattg atctggttgt ttttattcgc
    15721 tgtttatcta tttttatttc taacgcattc gatcatcgtg tagccgatca gagtccgatt
    15781 acgatcaatc tgctggaaga aaaagaggga tcacgtttta gtatagcgaa caaggcaaat
    15841 ccaacgaatt tgacgctatc ggagatggtc aaaagaggaa aaacctctaa aaaaaatggc
    15901 gggttaggtc tatacagtgc gaaaaaaatg ttggatgttt acgaaaatgc agaactaaaa
    15961 atcgaatttt caaaacagaa tcactatttt tttgtagaaa tgtatctggc atctagaaag
    16021 agcaaatcag cctactgact tactgtcaaa ggataagttt gaggaaagtc tacacggaga
    16081 tagagacaac aacacagcat attcaaaaga aacagaagcg tgatgaccgt tgtcaccctt
    16141 ctgtttcttt tgctatgtag ttaaacaaag tagttgacac ttccgctact tagtattagt
    16201 acgaaagtat acttcatgat actaagtaga taagaagtga aaagcggaac ccacgatcgt
    16261 caaaatgcta actgctttgc ttacaccaga taaagggaag agcaatatcg cttattttag
    16321 gcgtggagaa gcaagcaaat ccctacgatc acaaaaaaaa tgctgaccca gcagttaaga
    16381 gagttagaac aagatgaaag tatccatcga aaagcctata accaagtccc gcctaaagta
    16441 gagtactcgc tcaccgaaga aggaaaatca ttaagagaga ttctagtagc gttgtctgtt
    16501 tgggaagaag aacgtatcga aaaacaaaaa gcctcaaggg aagaagacac gctactgcat
    16561 acacatggtg gctacctaaa ttattgattt tctatgacat ttattttaaa taaaagaaaa
    16621 caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa
    16681 gtaagaagtg attggaagaa ggcaagcaaa tgaatagtat ggaaaaagtc aaccagttta
    16741 gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatc tctatttctt
    16801 tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa
    16861 cacaattaga acgaatcaaa gaagaactgg cagatgtatt gatctattca tacatgatgg
    16921 cagacaacct tcacctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt
    16981 tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttgtagaaat
    17041 gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag
    17101 aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg
    17161 ttattagtag gactagcttt gattttcaat gaacagatca aggattattt tgtcagagag
    17221 acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat
    17281 aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg
    17341 tcgcaaatga gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca
    17401 atcaatttac cggttttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca
    17461 ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg
    17521 aaccctgaat tgctgtttac accgctagaa aatttagaga tgggtgccaa gatctatttg
    17581 acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca
    17641 gatacccaat tactgaatga agtcgaggga aaagaaattg tcacactaat tacttgtggc
    17701 gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacatcgata
    17761 aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat
    17821 actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa
    17881 accaatttgc tgaaaaaaag agaaacgcaa agaggtgcat ctctgatttg gttgttttct
    17941 agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa
    18001 ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca
    18061 gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga
    18121 agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc
    18181 ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaagat tggggacgag
    18241 atttcgccag aagcggtcca aaaagaattt gttttaagag attcttttgc taaaaaccaa
    18301 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    18361 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    18421 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    18481 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    18541 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    18601 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    18661 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    18721 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    18781 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    18841 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    18901 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    18961 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    19021 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    19081 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    19141 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    19201 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    19261 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    19321 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    19381 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    19441 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    19501 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    19561 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    19621 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    19681 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    19741 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    19801 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    19861 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    19921 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    19981 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    20041 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    20101 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    20161 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    20221 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    20281 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    20341 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    20401 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    20461 aatcaattag aaaacaaaca tgcgaatgca ggaaaaccga ttacttcagc acggcatacc
    20521 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    20581 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    20641 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    20701 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    20761 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    20821 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    20881 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    20941 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    21001 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    21061 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    21121 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    21181 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    21241 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    21301 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    21361 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    21421 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    21481 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    21541 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    21601 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    21661 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    21721 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    21781 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    21841 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    21901 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    21961 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    22021 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    22081 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    22141 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    22201 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    22261 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    22321 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    22381 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    22441 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    22501 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    22561 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    22621 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    22681 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    22741 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    22801 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    22861 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    22921 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    22981 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    23041 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    23101 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    23161 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    23221 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    23281 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    23341 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    23401 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    23461 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    23521 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    23581 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    23641 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    23701 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    23761 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    23821 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    23881 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    23941 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    24001 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    24061 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    24121 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    24181 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    24241 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    24301 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    24361 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    24421 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    24481 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    24541 cacgttattt atttttgttt tatctataaa atggctgaa
//
LOCUS       DANJJH010000035        24575 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000035 DANJJH010000000
VERSION     DANJJH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(152..550)
                     /locus_tag="PWS53_001343"
     CDS             complement(152..550)
                     /locus_tag="PWS53_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL0871575.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="PWS53_001344"
     CDS             complement(547..1320)
                     /locus_tag="PWS53_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0871576.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="PWS53_001345"
     CDS             complement(1317..2000)
                     /locus_tag="PWS53_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0871577.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="PWS53_001346"
     CDS             complement(2167..3054)
                     /locus_tag="PWS53_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871578.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="PWS53_001347"
     CDS             complement(3051..3920)
                     /locus_tag="PWS53_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0871579.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="PWS53_001348"
     CDS             complement(4057..5343)
                     /locus_tag="PWS53_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871580.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="PWS53_001349"
     CDS             5616..6194
                     /locus_tag="PWS53_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0871581.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="PWS53_001350"
     CDS             6194..7123
                     /locus_tag="PWS53_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0871582.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="PWS53_001351"
     CDS             7120..8856
                     /locus_tag="PWS53_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0871583.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWS53_001352"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="PWS53_001352"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL0871584.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="PWS53_001353"
     CDS             complement(9752..10261)
                     /locus_tag="PWS53_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL0871585.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWS53_001354"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="PWS53_001354"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL0871586.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="PWS53_001355"
     CDS             complement(11562..12653)
                     /locus_tag="PWS53_001355"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL0871587.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWS53_001356"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="PWS53_001356"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL0871588.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWS53_001357"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="PWS53_001357"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL0871589.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWS53_001358"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="PWS53_001358"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL0871590.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="PWS53_001359"
     CDS             complement(16616..17590)
                     /locus_tag="PWS53_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL0871591.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="PWS53_001360"
     CDS             complement(17571..19454)
                     /locus_tag="PWS53_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL0871592.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="PWS53_001361"
     CDS             complement(19472..21697)
                     /locus_tag="PWS53_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL0871593.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="PWS53_001362"
     CDS             21812..22846
                     /locus_tag="PWS53_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871594.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="PWS53_001363"
     CDS             complement(22913..23926)
                     /locus_tag="PWS53_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0871595.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="PWS53_001364"
     CDS             complement(24004..24192)
                     /locus_tag="PWS53_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL0871596.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DANJJH010000036        23923 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000036 DANJJH010000000
VERSION     DANJJH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            153..530
                     /locus_tag="PWS53_001365"
     CDS             153..530
                     /locus_tag="PWS53_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0871597.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            518..856
                     /locus_tag="PWS53_001366"
                     /pseudo
     CDS             518..856
                     /locus_tag="PWS53_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            990..1433
                     /locus_tag="PWS53_001367"
     CDS             990..1433
                     /locus_tag="PWS53_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL0871598.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1694..2509
                     /locus_tag="PWS53_001368"
     CDS             1694..2509
                     /locus_tag="PWS53_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871599.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2519..3181
                     /locus_tag="PWS53_001369"
     CDS             2519..3181
                     /locus_tag="PWS53_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0871600.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3389..5053
                     /gene="alsS"
                     /locus_tag="PWS53_001370"
     CDS             3389..5053
                     /gene="alsS"
                     /locus_tag="PWS53_001370"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL0871601.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5066..5776
                     /gene="budA"
                     /locus_tag="PWS53_001371"
     CDS             5066..5776
                     /gene="budA"
                     /locus_tag="PWS53_001371"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL0871602.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5906..6403
                     /locus_tag="PWS53_001372"
     CDS             5906..6403
                     /locus_tag="PWS53_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL0871603.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            6588..6848
                     /locus_tag="PWS53_001373"
     CDS             6588..6848
                     /locus_tag="PWS53_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871604.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7210..7449)
                     /locus_tag="PWS53_001374"
     CDS             complement(7210..7449)
                     /locus_tag="PWS53_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871605.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            7735..8049
                     /locus_tag="PWS53_001375"
     CDS             7735..8049
                     /locus_tag="PWS53_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871606.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(8483..8849)
                     /gene="ssrA"
                     /locus_tag="PWS53_001376"
     tmRNA           complement(8483..8849)
                     /gene="ssrA"
                     /locus_tag="PWS53_001376"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9020..9793)
                     /locus_tag="PWS53_001377"
     CDS             complement(9020..9793)
                     /locus_tag="PWS53_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0871607.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            9937..10287
                     /locus_tag="PWS53_001378"
     CDS             9937..10287
                     /locus_tag="PWS53_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL0871608.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            10268..10984
                     /locus_tag="PWS53_001379"
     CDS             10268..10984
                     /locus_tag="PWS53_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871609.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            10984..12432
                     /locus_tag="PWS53_001380"
     CDS             10984..12432
                     /locus_tag="PWS53_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0871610.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(12502..13011)
                     /locus_tag="PWS53_001381"
     CDS             complement(12502..13011)
                     /locus_tag="PWS53_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871611.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(13001..13726)
                     /locus_tag="PWS53_001382"
     CDS             complement(13001..13726)
                     /locus_tag="PWS53_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871612.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(13949..16069)
                     /locus_tag="PWS53_001383"
     CDS             complement(13949..16069)
                     /locus_tag="PWS53_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0871613.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            16299..17477
                     /locus_tag="PWS53_001384"
     CDS             16299..17477
                     /locus_tag="PWS53_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL0871614.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            17493..18251
                     /gene="aroD"
                     /locus_tag="PWS53_001385"
     CDS             17493..18251
                     /gene="aroD"
                     /locus_tag="PWS53_001385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL0871615.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            18274..18759
                     /gene="ybaK"
                     /locus_tag="PWS53_001386"
     CDS             18274..18759
                     /gene="ybaK"
                     /locus_tag="PWS53_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL0871616.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(18830..20083)
                     /locus_tag="PWS53_001387"
     CDS             complement(18830..20083)
                     /locus_tag="PWS53_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0871617.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            20201..21550
                     /gene="gdhA"
                     /locus_tag="PWS53_001388"
     CDS             20201..21550
                     /gene="gdhA"
                     /locus_tag="PWS53_001388"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL0871618.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            21662..23008
                     /locus_tag="PWS53_001389"
     CDS             21662..23008
                     /locus_tag="PWS53_001389"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL0871619.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(23316..23702)
                     /locus_tag="PWS53_001390"
     CDS             complement(23316..23702)
                     /locus_tag="PWS53_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0871620.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(23751..>23923)
                     /locus_tag="PWS53_001391"
     CDS             complement(23751..>23923)
                     /locus_tag="PWS53_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL0871621.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         7858 a         4056 c         4830 g         7179 t
ORIGIN      
        1 cttttttatt tgaaataata cacaaacaac cgatatagat aatccagctt agttttaata
       61 gaaatgagta ttgacttgga gtacacttaa agttgtagaa taaataaaat acataagtaa
      121 agtggcaaga aattaagaga aagagtgatc agatgaatat caaacaagtc agtgaagaaa
      181 agggcatttc ggccgataca ttgcgctatt atgaacgaat cgggttgatt ccaccagtta
      241 atcggacaaa aggcggaatc cgtgattata cagaagaaga cctaaaatgg gtcgacttta
      301 cgatctgtat gcgaaatgcc ggattatcga tcgagtcttt gattgaatat attcgcctat
      361 ctagcgaagg agaagcaact gtatttgagc ggcgtaagct tttgattgaa gaaagtgaac
      421 ttttgacgaa aaaaattgct gaaatgcaag aatgtttgga acgtttacaa ggaaaaatca
      481 agaaatatga tcgtgtgtta tctagaaata aaatggaatg tatcaagtaa aaaaatcaag
      541 gagcatcgct atggagttac gcttactaaa atatttttgg acaattgcag aagaaggaac
      601 aatctctcaa gccgcagaag ttctacatct tacacaacca accttaagcc gacaactgaa
      661 ggagttagaa gagtaactag gaacagaact gtttgtacgc gaaaaccgca aaatgatctt
      721 gacagaagca ggttattttc taaaaaagcc gagcagaaga aattttagat ttaacgaatc
      781 aaaccactca agaattcgag aacagaaaaa aacaattatt cagcggccga tttacgatcg
      841 gctgtgtgga agctgatagt tcgaatacat tggcactatt actagaaaag ttgatccgtg
      901 attatcccag cgttcaattt catatccaca gcggaacaag tgatgatatc acggagtgac
      961 tagaaaaaag cctgttagat ttagcgattt tgctagatcc agtagataca gagaaatacg
     1021 aaacgatttt gttaccaaga aaggaaaaat ggggattgct cattttagaa aattctgttt
     1081 tagcaaaaaa agacgtgata acaaaagaag actttgcgaa tgtacctctt ttgatctcaa
     1141 agcgtccaga aatacagcga atgtttgctg actggttagg cataaaaaag gagcagttgc
     1201 tgatcacagg aacgtacaat ttgattttca atgtatttgc gctaattgag aatcaggtag
     1261 gtgctgcttt agtcatagaa ggtgctgtaa gtcatcattc aaagcgagtg acattcgttc
     1321 cgcttgaccc aatgattgaa acgaactgca tgctagtctg gaaaaagaat cggattttat
     1381 cacctgtagt caatgaattg attcaccgtt tcaaagaagc ttttgaacag taataagtat
     1441 ctctaatcga agaaatttca atacaaaagc gagacgtatg acagtcttgc ttttgtattt
     1501 tttcattttt aaatcaaaaa aataaagaga tttacataac ttgttaaata agcgcttaca
     1561 aaaataagaa gaacggaaat gaatcagcta taataaccgt tgttctggat tgtaatcatt
     1621 acaaagaaat gaagattata actttggagg tgttcgaaat gaaagaaaaa aagcaaaaat
     1681 tcgttcaaaa aacatgccct ttattcaatg gttgggtatg attctgctga ttgtattagc
     1741 tttattgacg atcattcctt ttttatggat ggtttctgct tcattcaaag caaataatga
     1801 agtgttcacg attccgattc aatggatccc taaaacttgg catttagaga attatctggt
     1861 tatttggtct cgtattcctt tagtgacatt ttttaagaat acagcttttt taagcgttgt
     1921 gatcacgttg attcagttgt ttacttctag ttttgctgct tacgggtttt ctaaactaaa
     1981 ttttaaaggg cgcgatattt tatttttagc atatatcggc acgattgctg tcccttggca
     2041 gtcttatatg atcccgcagt tcattatgat gagaaaagtg ggtttaacag atacgttgtg
     2101 gtcgttggtg ctattacaag cgttcaatgc ttttggtgtg ttcttgttga agcaatatta
     2161 cagtagtatc ccagattctt tatgtgagag tgcgcggatc gatggcttat cagagtgggg
     2221 aatctatcgc aagatcattt tacctcttac aaaaccggcg attgccagtt tgacgatcat
     2281 cacattcgtt aatacatgga acgattatat ggggccattc atttatttgt cttctacgga
     2341 aaataaaaca atccagttag ggttgaaaat gtttattggg ctatttgatg cagagtacgc
     2401 attaatcatg gctgcctcag ttgtttctgt tcttcctgtt gcttttatat ttttagcgat
     2461 gcaaaaatat tttgtagaag ggatcgcttc atcaggaatg aaggaatagg aggtgattat
     2521 gaaagagcag cgttttgaca aaaatgttta tatggaattc ttttattggt tgtatcaatc
     2581 attggcaagt agcctttgtt tttggtgtgt caatatcttg tatatagcag ctgcgttcct
     2641 tttgtcgata gatggaagga atgtcttttc ctttattttt tctttacttt ttattggtcc
     2701 aggttgcatc gtgatgatag gtatgacaga agaattagca aaagaaaata gtttttcacc
     2761 agtacgtttg ttcataaaga tgtttcgcca attttggcta aagggatttt tttattggct
     2821 atttgcttgg atagttagtg tgataatgat ttttgattgt ttctttttta tacgtttttc
     2881 ttatggaaaa tggttgattc cgttgtttgt tttacttgct tgtttatcag tttcctttag
     2941 caccaactgc tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc aagttctacg
     3001 gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt ttactagatt ttttactatt
     3061 gacaagtttg tttctcttct ttttcgtaaa gccccaatgg tgtattctac taggtccttc
     3121 tatcgtattt ggtttgattt acttcaacaa tcgtaaattg atgagaacta tggatctgta
     3181 gactttataa attgaaactt tcaattaaga atgacagttt tttttatgta aaaactgtca
     3241 ttctttttca ttttttgaaa catatggcta aacaaatgaa ataaaacgtg ctatactttt
     3301 gtatgaaaca ggtttagaaa ctgtatcact tatttgtgat tatttctaac taataattaa
     3361 aaataaagca taaaaaggac gatgttcaat ggctgaaaaa atgaaacaag gttcgaaatc
     3421 tgtagtagaa agtttgatta atcatcatgt tgattatgta ttcggtatac caggtgcaaa
     3481 aatcgatggt gtatttaatg aattagagga tcaaggtcct gaattaatcg tgactcgtca
     3541 cgaacaaaat gctgcattta tggcacaagc aatcggacgt attacaggag agccaggagt
     3601 agttatcgca acaagtggtc ctggtgctag taacttagct acaggtttgg taacagcgac
     3661 agcagaaggt gacccagttt tagcaattgc cggacaagta aaacgaagcg atctattgaa
     3721 gttgacccat caaagcatgg ataatgcagc actgttccag ccaatcacaa aatacagtgc
     3781 agaaatccaa gatccagaaa caatttcaga agtgattgcc aatgcttatc gtatggctaa
     3841 gagttcaaaa aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg atgcacctgt
     3901 ccaaggtaat gtcatcaagc cattgagcga tcctaaatta ggttctgctt ctgcagatga
     3961 tatccgttac ctagctgaac gcattagaga agcaaaacta ccggtactat tagtaggtat
     4021 gcgaggatca agtgaaaaag aaacgttagc catcaggcaa ttagttgaaa agacagcatt
     4081 gcctgttgta gagacattcc aagcagctgg agtgatctca cgtgaattag aagcgcattt
     4141 ctttggtcgt gtgggtttat ttagaaacca accaggagat atgcttttga aacgaagtga
     4201 tctagttatt gcaattggct atgatccaat cgaatatgaa gcaagaaact ggaatgcgga
     4261 aaaagatgcg cggatcattg tgatcgatga agcgccagct gaaatcgatc cttttatgca
     4321 gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga caggttcatt
     4381 agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc aagcaaaatt
     4441 aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc ctttagaagt
     4501 tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag atgtaggtag
     4561 tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt tgttattcag
     4621 taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg cattggtgcg
     4681 tccaaataca caaatcgtat cagtttccgg cgatggggga tttttattct ctgcccagga
     4741 tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga atgatggaca
     4801 ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag gtgtagactt
     4861 tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa tccgagctac
     4921 aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag gacccgtaat
     4981 tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa tcttaccaga
     5041 tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc acgggacatt
     5101 aggagctttg atggccggct tgatggatgg aacagaaacg attgctcata tattagaaaa
     5161 aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct ttttagatgg
     5221 cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa gtgaacttac
     5281 cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct caaaaactgc
     5341 agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa acctattttt
     5401 ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc cgaagcaaga
     5461 aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc aacaaagcaa
     5521 tatccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag ttgctgcagc
     5581 gggatttcac ctgcatttta tcgatgatac acatacattt ggcggacatg taatggattt
     5641 tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac agcattttcc
     5701 agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag cagcagaaat
     5761 ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac ctgacgtatc
     5821 tcgttttttt gaggaggaaa cctagcgttt acaaaaaaac tgagctataa tcttctccat
     5881 agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag atgcatacga
     5941 aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt tagcaaattt
     6001 agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg caggatatta
     6061 tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt cttgcacacg
     6121 tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa cattgatcgt
     6181 tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac aatcagaaat
     6241 cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg attgcggagt
     6301 aacgaaaggg tatgatgaaa tcgatcaagc atttctagaa aaatatgtag atcttttatt
     6361 agcaaatttt aatataaatg ctttttcatt tattaataac taaaatcgtc tattgaaaca
     6421 aaaaagaaga gaaatcaaga ataaaaagag ccggaaagac agtggtcatt tctgtttttc
     6481 ggttttttta tttttttata aaatcacaag tgttattgat tttcacaatc atattttaat
     6541 ggtattatca ttttgaataa tgaaaaaaca ttaaggatgg atgaaatatg aataacgaaa
     6601 ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa aaagtggttg
     6661 gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa tatcatgacg
     6721 tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat actcacgttt
     6781 atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa catactttag
     6841 tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt ttgaaggcac
     6901 tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt tcgcctagag
     6961 aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata ctgccagaac
     7021 tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat ccaatcattt
     7081 caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg taaagcattt
     7141 gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt gctgaagaag
     7201 atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca gtttctttac
     7261 cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag aatttttctc
     7321 atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag gtatgttctt
     7381 tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga taaacagaat
     7441 tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat gctcagtatt
     7501 aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct tagtaataca
     7561 tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat ctgtattaac
     7621 gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg taggtatgat
     7681 attatctaga ggtaattttt ttgggtattt aaatagttgc taggaggata gcttttggat
     7741 ttttacagta attttatctt aataatagca atattattac ttctaaatat ttggtttttt
     7801 gataagagta gaaatgcagg gataggtttt agaaccaaac gaagtacgtc ttctgaaaaa
     7861 aagtgggtat attcacagac tattttttac ggaggggtta tttcaatcag tttactttct
     7921 tcaacacttt attcttttaa tgttatagac gtttccatgt caaattttat atctattatt
     7981 ggaatcctta tttctgcaat tattacacaa ctacttcttg tgtttgaaga gaagtcaaaa
     8041 aataattaaa gcaattatgc cttttagtac aggaattatt acttcttaca aatgtatagc
     8101 gaatgtgaag cctgctgttt taattgttct agaaaaactt ttgagtgaac aattatttta
     8161 tgttaggatg aattaatttc tctagagttt ataattaaaa aacttatttg aaggaacatg
     8221 cttaaaccat aattgtgttt ttccttccat cagcataaaa gttcacctca aaaagagtgt
     8281 acgaacaaaa gttctttttg taaaccttaa aatagatttt cctgaatacg tattggggat
     8341 ttctaaatgt ttgaatgttt ttttgaatgc aaaatcaatg caaaaaattt tgtatttctc
     8401 tacttttctc tccttgacca aaataaaaaa acgttgattt aacaacgttt ttcatacatg
     8461 tttatctgtc tcaaccatac atatatggag acggcgggag tcgaacccgc gtccaaacac
     8521 atcgccactt aaaaatctac gttcatagtc aactcattta aggattcact ttacagcttg
     8581 ccgagtgaca ggcattctgt atcgctagtc tgatgttctc tttttcaact tacagacgga
     8641 aagttgaaac gtatcccact aaagttagga cccttactcg agcacatggg cgatgccgag
     8701 aggatctacg ctaactgttt ttaggcagct aaagcgtaag agttgttttc gtttttagca
     8761 gttatattta actgtaacgt ttttacgtag acgtaaccta cgaaacgcaa ttcaagctcg
     8821 actgtgcctg tcgaatccgt aacgtccccg ttagctaaca agggttacgt gtatttttca
     8881 tacaaaatta gtatagcata tattgggata aattcaataa aaaagcgaat catatcccat
     8941 tatttatata ataactgccc ctttcttaag aaaaaataag cgattttctt aagaaaagag
     9001 gtagcttcaa aaaaagatct taaatcagat caaaatactt caatgcgtgg acaattccgc
     9061 cttctgtatt tttctttgtg ataaaggtag aaagttcttt taatccgtca taggcgttgc
     9121 ccatagcgat tttgttgtcg caagcttcca acaaggcaaa atcattcgga ccatcgccaa
     9181 atccaaaagt aggaactttt tccagctgaa gttctttttg cagacgtttg acacctgccc
     9241 cttttgagat tcctttcttc actgtatcga tcgagtaagg accattccga taaaaagtta
     9301 gctcaggaaa atgttcgtga tagtattcgt cacctgattg agataagatc agcaacatat
     9361 ttactgtgtt ttctttaaag cctaaaggat caatttccgg aacatctgaa tgaatgaagt
     9421 cgtaagcatt gatcattgtt tggctatgac cggtacacca gattttctga tcgttatagt
     9481 aagaaagttc atgaccgttc aactgtacat gatcatacat tttttgacat tcttctgaag
     9541 aaaatgtttg agaatagatt tcttcgccgt ctactcgaat gaaagaacca ttcatggtga
     9601 ccgcagaagt aatgcccgca tcttccatga tagagtggat ttctgcttct gtacgtcctg
     9661 tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt catagctttt gccacttcgg
     9721 gtgtgatttt tgatttcgca tctagtaatg ttccatcaag atcaaagaaa gtgattgcac
     9781 gataatttga cacaaataaa actccttttt tattggaata agtttatctt aacaaagaat
     9841 gaacaaaaga caaacctttc gtgaagaatt cctgaacata ttggttcagt caacaattta
     9901 gagtatacta taagaagaga atttaggagg gaaagaatga tttttttcag tattggtatc
     9961 ttattcttaa taattggcag tctttttcgg attctgccct caaaaggaaa tctgcctttt
    10021 tatgggtatc attcgcctct tgccgcaaaa acagacgctc attggcggct ggctcaaaag
    10081 acaagcggaa actggttttt tcttatgggc ttactgatgg cattgatcgg ctattattta
    10141 aagacatctg ggcatacgaa ttattttctg attgaaatgc ttctgctagt atttccaatt
    10201 atgcctattt ttattatgac agaaaagaaa ttacagaaat atgacctgga aactggaggg
    10261 aacgataatg aatattttaa tgattgaaga taatcaatct gtttctgaaa tgatgcagat
    10321 gtttttctta aatgaaggtt gggaagcaac tttcaagtat gatggaaaag aagggctcga
    10381 tgccttttta gaaaacccac aacattggga tatgattaca ctagacctga atttgcccac
    10441 actggacggt atgctagtag gaagagagat ccgcaaagtt tcaagtactg tgccgattat
    10501 catgctgaca gctagagatt ctgaaagcga tcaggtgatc ggcttagaga tgggggcaga
    10561 tgattatgtc acaaaacctt tcagtcctct gacgcttatt gctcgaatga aagctttgca
    10621 tcgccgtagt gagttggcag aagcaagaga agaagcagca gtttcagatg atcattttga
    10681 tatcgagact agtcatttca agatgaatac gaaaacacga gaagcgtatc tggacaaccg
    10741 gccaattgaa ggactgacgc ctaaagagtt cgatctattg tttactttag caaaaaaacc
    10801 acgtcaagta ttctctcggg agcagttgct tgaattagtc tgggattatc agtactttgg
    10861 tgatgaacgg acagtggacg cccatatcaa gaaattacga caaaaaatcg aaaaggtcgg
    10921 accacaagtc atccaaactg tttggggagt cggctacaaa ttcgatgatt ctggtgttgc
    10981 ctaatgcgtt atttgtatca gcaactctta gctttttgcg gaatggtcgc gttgatctta
    11041 ttgatcgttg gagtttcttt tacacaactg acaaaacaaa cgattgaaga aaacaattac
    11101 cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa cgcaaaccta tgccgatacg
    11161 ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat tgctgctgac agagcaagta
    11221 ctgacagaac aaaatgtgaa ctttatattt atcgataaaa acgaacgtgt catttatccg
    11281 actaatacag ctttactgaa ttttacctta acagctgatc aatggaaaaa tctgaggaat
    11341 ggacgtcaag aaaaattcac atcgaataaa aatattttag gacaagcaca ggcgacctct
    11401 tacgcacttg tgccatttaa tttgaatcat gaattctatg gagcacttgt agttagtcaa
    11461 cctgcgcgaa atatcgataa cagcgttcgt ccaattacac tgaacctgtt caaaggattt
    11521 attttttcca gtatcgttgc agttattgcc agctatgctt ttgcagcatt ccaagttaag
    11581 cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga atggtaattt tgatgtacag
    11641 ctccccgtac atgacaaaga tgaatttgat gagttagcag atgatttcaa taaaatgacg
    11701 aattcattga aagaatctca agcagaaatt gaagaacaag aaaatcgtcg aagacaattt
    11761 atggcagatg cttctcacga gatgagaaca cctcttacga caatcaacgg acttctggaa
    11821 gggttagaat acaatgcaat ccctgaaaat cagcgagaaa acgcgattaa attgatgaaa
    11881 aatgaaacag agcgtctgat ccgtttggtg aatgaaaact tggattatga aaaaatccgt
    11941 acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa cagaaacatt gaaaaatatt
    12001 gttgcacagt tagaagctaa agcagaagca gcaggagata cattgacatt gaaagccgtt
    12061 gatgatattg atgtttacgc tgattatgac cgatttgtcc aaatcatggt caatattatt
    12121 caaaacgcga tacagtttac tgaaaacggc caaatcacga ttactttaga aaaaggctat
    12181 cttgaaacga tcatcacgat tgaagatacg ggaattggaa tgtctgaaca acaaatgaaa
    12241 agtatctggg atcgttatta taaagtagat ccttcaagaa aaaatacaaa atatggtgaa
    12301 tcaggattag gtttaccaat cgtgcagcaa cttgtacgct tgcataaagg aaaattagaa
    12361 gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc gtttgcctga cgtagaaatc
    12421 aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat cgatcttcta taccgcttgt
    12481 ttttactata aaatgattga tttagcccgc gggctctaaa cgatcaatat aaggactttg
    12541 agcaatagta gcatcatgag taaagagtaa gataccttga ttttcttcat gagctaaata
    12601 agaaagcaag tggagtagct cttggatctc caatacacta agttcatcaa atgtatcttc
    12661 aataacaata ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct ttactttttc
    12721 aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa ggagagatat caagtttttc
    12781 ttgataggcc aaacgatctt gtttgatttc tttttccggt acatccaaca ggacatggtc
    12841 tttaagattc agataaggaa acatcggcca atttttttgg atgatcgcaa aattatccag
    12901 ttttgcaaca agaacctctt ttttcaagtg ctccaatatt ttatgctgtt tttcgagagg
    12961 acacaaaata acggtcaagt gttcagcatg aatcaaatat tcaggtttca ttacgtccag
    13021 gcctcctact ttgcaaatga tggataaaaa aagtgaaagg gaatagacta agaatgagac
    13081 agcatccact gagcccaata agagtttggg caaatccctt aagtatatct gtgaaaaaaa
    13141 gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt gtaagtccgc
    13201 ttaccgaagt caagatatca cggatatctg gcagttcagt agtctttaag acaagaaaat
    13261 tgatcctttg aagaagcgct tcataaactg actggaagct tacgatccac aataaaacca
    13321 agatacaaca aagaaagaga ggaaagaaca gctgccagaa aataaaaagg aataatttgg
    13381 ttttagaaag accagtcagg cgccaattga tcaattcctg cttgttttgc ctgactgaat
    13441 agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa taaactgaat atgcctttta
    13501 tataaaatcg gctagctaat tctaaagccg gaagaagctc tgtagatgca gaagcgccaa
    13561 taaatgaaag ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg taaagagtca
    13621 ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa acaatagagt attgtctgct
    13681 ttttgtaata aattaatcga gcagtagcat agcggattat tttcatactt ttcacctcta
    13741 taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt cctttagtga aatcacgaat
    13801 taaaaccaga aaatattttg taaaaagcaa gaggttgtga agaagctgcc ctattctatt
    13861 accgtagatg caagcttttg aacactgttt attcgaaaag aggagcagcg acaataattt
    13921 tgtcgctgct ccttttaaat ttattttgtc attcggaaga acttgtggtc gttccgtgaa
    13981 gttcttggta cgtttgcgtt ttataaagat caacagttga ttgtccgttg tgttcactaa
    14041 agacactagt tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat
    14101 tcttgtagtc ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa
    14161 gaagatcacc attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca
    14221 gtgaatcaat ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt
    14281 tactatagat cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga
    14341 aagaaacaag ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt
    14401 ttgtatctac acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag
    14461 tgtgattgat tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca
    14521 tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat
    14581 ttttatttct tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat
    14641 catacaagcc gctttctttc aaataattga agaattcttg tacggcggta tctaaataat
    14701 tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg
    14761 cttcgtcatt ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat
    14821 aaaatggttg ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat
    14881 gcaacccgta ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat
    14941 aatcatatcc taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat
    15001 ggaataccgc actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag
    15061 tattttttcc gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt
    15121 ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa
    15181 aactgaacgt gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcatgctgga
    15241 gaccattctc atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga
    15301 tataaatcac attttttcct ttagcgagcc cgaacaaatc gtcatttggt gctgcataat
    15361 gttcttttac ataattttct acttctttca tatcattggg acttgcttct gcccgcactt
    15421 ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt
    15481 tgaccaaata atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca
    15541 taaaaagatt tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg
    15601 ctcggatcgg gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa
    15661 taaaatcgat ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca
    15721 gaccactcgc tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt
    15781 ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca
    15841 tcgtgatata agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg
    15901 tagttgcaat agggttgata aacaagataa agtattgaat gccactttcg attccgagat
    15961 ggaaatcaac taaataggca aaaatcgttt tcaaccaaag taaaacgact agtaaagaaa
    16021 aaaatccgag cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc
    16081 tttctgcact tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct
    16141 catttatttt actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc
    16201 attaaggttt tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt
    16261 gttatacttg ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa
    16321 gagcaagcaa tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc
    16381 cccaaaaaga aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa
    16441 aaggatatct aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc
    16501 catttgatca atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct
    16561 ttttttcaga tgattctact accgcttttc gtatattcaa tggagaagga gacggaatcg
    16621 gcggtctgat tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt
    16681 tatatgcaca tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag
    16741 gaggctatga gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg
    16801 gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt
    16861 taaatgaagg attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag
    16921 cagaaggttt cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt
    16981 ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca
    17041 gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa
    17101 tcattgttat ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg
    17161 acatgattgt attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag
    17221 caaaagatta tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac
    17281 ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag
    17341 aggaattcca tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag
    17401 atttccagac gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa
    17461 ttaaaaataa ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag
    17521 gaagcggtag accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac
    17581 gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt
    17641 atcgtgaagt tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc
    17701 ttgatggact gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca
    17761 tatcgatccg tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata
    17821 tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca
    17881 agtctttaaa cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc
    17941 ctgtcttgct ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg
    18001 cagtaactcc caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa
    18061 atgcttttgt ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc
    18121 gtctggctgg cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta
    18181 cttctgctcc aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg
    18241 aaccgttgta gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg
    18301 aatgcaatcc gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg
    18361 gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcgt
    18421 atatttaaaa cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg
    18481 ggagaagccg aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa
    18541 atgctccatt taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct
    18601 ataggtatga aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa
    18661 atcattgtct cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt
    18721 gcattaacga gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga
    18781 aggaggccgg gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt
    18841 gcccggcatt cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc
    18901 cagtataacc attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac
    18961 tctccaatgt tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt
    19021 tctcaatagg aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat
    19081 cttgaatttg tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa
    19141 gaaagaaaac taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt
    19201 tggttgcttt acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga
    19261 aacaatccag agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac
    19321 ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg
    19381 tatgttcagt caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag
    19441 aaagcccttg aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg
    19501 tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag
    19561 tagaaccagt agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga
    19621 actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta
    19681 ctcgagtaga gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga
    19741 taatcgtttt tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat
    19801 gtacgccttg tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac
    19861 tatataaaaa ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt
    19921 tcgtaacatt gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt
    19981 tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc
    20041 ttgttccagc gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa
    20101 agtgtagcat atctgaagaa agaaaacaga aaaaaaatgt tttgagccaa aagagaaaat
    20161 gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata
    20221 tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca
    20281 agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat
    20341 cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc
    20401 ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc
    20461 agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt
    20521 gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg
    20581 aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt
    20641 ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc
    20701 gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt
    20761 gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat
    20821 acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga
    20881 aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta
    20941 tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg
    21001 gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa
    21061 tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga
    21121 atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga
    21181 aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg
    21241 cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta
    21301 ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca
    21361 gaacgtccag cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat
    21421 ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct
    21481 gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg
    21541 tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga
    21601 aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca
    21661 aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt
    21721 agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc
    21781 aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc
    21841 acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg
    21901 tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt
    21961 caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat
    22021 cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa
    22081 cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga
    22141 gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga
    22201 taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat
    22261 tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc
    22321 tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata
    22381 ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct
    22441 ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt
    22501 ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta
    22561 tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg
    22621 acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat
    22681 tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt
    22741 cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa
    22801 cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt
    22861 tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg
    22921 tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct
    22981 tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca
    23041 gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata
    23101 ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg
    23161 tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag
    23221 gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac
    23281 tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt
    23341 acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt
    23401 gctttcaact gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa
    23461 gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg
    23521 tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc
    23581 attggagctt tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc
    23641 caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc
    23701 atgaaagatc tcctttttta tttttattat acccgtggtg ctagttaatt ttaatatcct
    23761 aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag aacactgtaa
    23821 gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga gttgaaattt
    23881 tgacaaccta atttttctaa ggaagaaaaa acagtctcaa ctg
//
LOCUS       DANJJH010000037        23832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000037 DANJJH010000000
VERSION     DANJJH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(56..790)
                     /locus_tag="PWS53_001392"
     CDS             complement(56..790)
                     /locus_tag="PWS53_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0871622.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="PWS53_001393"
     CDS             1117..1527
                     /locus_tag="PWS53_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL0871623.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="PWS53_001394"
     CDS             1520..2221
                     /locus_tag="PWS53_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL0871624.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="PWS53_001395"
     CDS             2221..3807
                     /locus_tag="PWS53_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871625.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="PWS53_001396"
     CDS             3826..4047
                     /locus_tag="PWS53_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL0871626.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="PWS53_001397"
     CDS             4044..4805
                     /locus_tag="PWS53_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL0871627.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="PWS53_001398"
     CDS             5185..5694
                     /locus_tag="PWS53_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0871628.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="PWS53_001399"
     CDS             complement(5769..6308)
                     /locus_tag="PWS53_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL0871629.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWS53_001400"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="PWS53_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL0871630.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="PWS53_001401"
     CDS             7321..8172
                     /locus_tag="PWS53_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL0871631.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="PWS53_001402"
     CDS             8200..8835
                     /locus_tag="PWS53_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL0871632.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="PWS53_001403"
     CDS             8855..9628
                     /locus_tag="PWS53_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0871633.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="PWS53_001404"
     CDS             10036..10833
                     /locus_tag="PWS53_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL0871634.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="PWS53_001405"
     CDS             10811..11224
                     /locus_tag="PWS53_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0871635.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="PWS53_001406"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="PWS53_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0871636.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="PWS53_001407"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="PWS53_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL0871637.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="PWS53_001408"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="PWS53_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL0871638.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWS53_001409"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="PWS53_001409"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL0871639.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="PWS53_001410"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="PWS53_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL0871640.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="PWS53_001411"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="PWS53_001411"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL0871641.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="PWS53_001412"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="PWS53_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL0871642.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="PWS53_001413"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="PWS53_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL0871643.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="PWS53_001414"
     CDS             21908..22486
                     /locus_tag="PWS53_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0871644.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="PWS53_001415"
     CDS             22538..23020
                     /locus_tag="PWS53_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL0871645.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="PWS53_001416"
     CDS             23137..23511
                     /locus_tag="PWS53_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871646.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4285 c         5114 g         6649 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tt
//
LOCUS       DANJJH010000038        23594 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000038 DANJJH010000000
VERSION     DANJJH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            37..1377
                     /locus_tag="PWS53_001417"
     CDS             37..1377
                     /locus_tag="PWS53_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0871647.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1595..2038
                     /locus_tag="PWS53_001418"
     CDS             1595..2038
                     /locus_tag="PWS53_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL0871648.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="PWS53_001419"
     CDS             complement(2542..3960)
                     /gene="nhaC"
                     /locus_tag="PWS53_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0871649.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4363..5586)
                     /locus_tag="PWS53_001420"
     CDS             complement(4363..5586)
                     /locus_tag="PWS53_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL0871650.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5617..6456)
                     /locus_tag="PWS53_001421"
     CDS             complement(5617..6456)
                     /locus_tag="PWS53_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0871651.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6473..7153)
                     /locus_tag="PWS53_001422"
     CDS             complement(6473..7153)
                     /locus_tag="PWS53_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871652.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7155..8228)
                     /locus_tag="PWS53_001423"
     CDS             complement(7155..8228)
                     /locus_tag="PWS53_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871653.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8284..8522)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8565..8798)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8875..9546)
                     /locus_tag="PWS53_001424"
     CDS             complement(8875..9546)
                     /locus_tag="PWS53_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0871654.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="PWS53_001425"
     CDS             complement(9633..12311)
                     /gene="mgtA"
                     /locus_tag="PWS53_001425"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL0871655.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12970..13407
                     /gene="ndk"
                     /locus_tag="PWS53_001426"
     CDS             12970..13407
                     /gene="ndk"
                     /locus_tag="PWS53_001426"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL0871656.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13429..14295)
                     /locus_tag="PWS53_001427"
     CDS             complement(13429..14295)
                     /locus_tag="PWS53_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0871657.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14455..16548)
                     /locus_tag="PWS53_001428"
     CDS             complement(14455..16548)
                     /locus_tag="PWS53_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0871658.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17297..17920)
                     /locus_tag="PWS53_001429"
     CDS             complement(17297..17920)
                     /locus_tag="PWS53_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871659.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18079..19476)
                     /locus_tag="PWS53_001430"
     CDS             complement(18079..19476)
                     /locus_tag="PWS53_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0871660.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19508..20479)
                     /locus_tag="PWS53_001431"
     CDS             complement(19508..20479)
                     /locus_tag="PWS53_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871661.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20853..21278
                     /locus_tag="PWS53_001432"
     CDS             20853..21278
                     /locus_tag="PWS53_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871662.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21766..22467)
                     /locus_tag="PWS53_001433"
     CDS             complement(21766..22467)
                     /locus_tag="PWS53_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0871663.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22483..22923)
                     /locus_tag="PWS53_001434"
     CDS             complement(22483..22923)
                     /locus_tag="PWS53_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0871664.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22923..23498)
                     /locus_tag="PWS53_001435"
     CDS             complement(22923..23498)
                     /locus_tag="PWS53_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL0871665.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6784 a         4895 c         4063 g         7852 t
ORIGIN      
        1 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
       61 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
      121 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
      181 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
      241 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
      301 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
      361 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
      421 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
      481 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
      541 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
      601 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
      661 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
      721 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
      781 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
      841 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
      901 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
      961 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
     1021 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
     1081 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
     1141 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
     1201 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
     1261 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
     1321 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
     1381 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
     1441 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
     1501 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
     1561 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
     1621 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
     1681 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
     1741 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
     1801 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
     1861 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
     1921 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
     1981 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
     2041 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
     2101 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
     2161 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
     2221 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
     2281 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
     2341 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
     2401 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
     2461 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
     2521 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
     2581 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
     2641 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
     2701 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
     2761 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
     2821 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
     2881 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
     2941 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
     3001 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
     3061 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
     3121 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
     3181 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
     3241 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
     3301 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
     3361 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
     3421 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
     3481 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
     3541 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
     3601 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
     3661 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
     3721 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
     3781 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
     3841 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
     3901 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
     3961 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
     4021 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
     4081 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
     4141 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
     4201 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
     4261 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
     4321 gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc
     4381 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
     4441 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
     4501 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
     4561 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
     4621 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
     4681 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
     4741 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
     4801 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
     4861 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
     4921 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
     4981 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
     5041 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg
     5101 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
     5161 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
     5221 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
     5281 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
     5341 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
     5401 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
     5461 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
     5521 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
     5581 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
     5641 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
     5701 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
     5761 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
     5821 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
     5881 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
     5941 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
     6001 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
     6061 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
     6121 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
     6181 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
     6241 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
     6301 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
     6361 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
     6421 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
     6481 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
     6541 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
     6601 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
     6661 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
     6721 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
     6781 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
     6841 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
     6901 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
     6961 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
     7021 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
     7081 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
     7141 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
     7201 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
     7261 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
     7321 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
     7381 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
     7441 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
     7501 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
     7561 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
     7621 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
     7681 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
     7741 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
     7801 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
     7861 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
     7921 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
     7981 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
     8041 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
     8101 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
     8161 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
     8221 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
     8281 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
     8341 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
     8401 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
     8461 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
     8521 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
     8581 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag
     8641 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
     8701 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
     8761 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
     8821 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
     8881 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
     8941 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
     9001 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
     9061 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
     9121 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
     9181 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
     9241 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
     9301 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
     9361 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc
     9421 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
     9481 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
     9541 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
     9601 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
     9661 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
     9721 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga
     9781 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
     9841 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
     9901 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
     9961 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    10021 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    10081 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    10141 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    10201 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    10261 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    10321 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    10381 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    10441 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    10501 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    10561 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    10621 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    10681 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    10741 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    10801 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    10861 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    10921 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt
    10981 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    11041 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    11101 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    11161 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    11221 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    11281 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    11341 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    11401 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    11461 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    11521 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    11581 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    11641 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    11701 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    11761 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    11821 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    11881 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    11941 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    12001 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    12061 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    12121 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    12181 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    12241 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    12301 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    12361 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    12421 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    12481 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    12541 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    12601 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    12661 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    12721 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    12781 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    12841 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    12901 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    12961 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    13021 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    13081 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    13141 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    13201 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    13261 ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct
    13321 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    13381 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    13441 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    13501 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    13561 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    13621 atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    13681 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    13741 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    13801 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    13861 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    13921 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    13981 tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc
    14041 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    14101 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    14161 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    14221 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    14281 ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    14341 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    14401 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    14461 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    14521 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    14581 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    14641 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    14701 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    14761 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    14821 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    14881 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    14941 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    15001 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    15061 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    15121 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    15181 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    15241 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    15301 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    15361 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    15421 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    15481 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    15541 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    15601 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    15661 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    15721 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    15781 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    15841 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    15901 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    15961 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    16021 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    16081 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    16141 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    16201 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    16261 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    16321 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    16381 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    16441 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    16501 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    16561 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    16621 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    16681 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    16741 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    16801 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    16861 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    16921 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    16981 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    17041 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    17101 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    17161 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    17221 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    17281 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    17341 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    17401 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    17461 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    17521 aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca
    17581 aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa
    17641 gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca
    17701 attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt
    17761 cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag
    17821 caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac
    17881 cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt
    17941 aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag
    18001 atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt
    18061 gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca
    18121 atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc
    18181 atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata
    18241 gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac
    18301 aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt
    18361 ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac
    18421 attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat
    18481 tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt
    18541 atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag
    18601 atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc
    18661 aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag
    18721 gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga
    18781 ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc
    18841 catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg
    18901 gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc
    18961 gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc
    19021 aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc
    19081 tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc
    19141 gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt
    19201 tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc
    19261 agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc
    19321 aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt
    19381 ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc
    19441 ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta
    19501 gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    19561 cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg
    19621 ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat
    19681 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    19741 caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    19801 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga
    19861 tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt
    19921 ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga
    19981 tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt
    20041 ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc
    20101 actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa
    20161 ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    20221 tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc
    20281 cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt
    20341 tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca
    20401 gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca
    20461 catcacggat cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt
    20521 gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat
    20581 aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt
    20641 gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc
    20701 tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt
    20761 gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata
    20821 atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt
    20881 tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct
    20941 cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg
    21001 tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag
    21061 ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat
    21121 ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac gaatatatta
    21181 tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc
    21241 gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt
    21301 tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa
    21361 tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa
    21421 tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca
    21481 tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca
    21541 aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt
    21601 gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa
    21661 agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct
    21721 acatcacgta gtaatttttc tttcccgtcg tttggttttt tatttttagc ctaattcttg
    21781 accttttgca tgctggttat ccgtaaaata ttttccagca ggctttcctt ccaattcagc
    21841 taatttttct tggatctgac ggatcacctt ttttgcatct tctggatttt ctacaaaatc
    21901 gaatttatcc ccatccactt gtattttagg acatacatca aaatcttcat accattgatt
    21961 atagcgggtg ttcaacgttt cataataggt atataacgtc tcatcgcttt ctagctgttc
    22021 atagtcacgc ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg
    22081 gaccatcaaa tcaggccgtt tttttggagc aacatcatct aactcgttta gcatcgtatc
    22141 taataagcta tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcgtttag
    22201 atggaacaac aggctatctt catagatgga tcggtctagc acattgtcat cgttcactaa
    22261 agcatttttc attgctaaaa atcgcttgtt caaaaagaag atttgcaata aaaaagcgta
    22321 ctgctcagga tttgaataaa agagcggcaa tacttcgtta tcttcgacat tttcataaaa
    22381 tggtttagaa tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat
    22441 cgttcctgaa atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac
    22501 gtaagtttca acaggcaatg tttcaaaatc atattcacgt acagcttggt ctgattttaa
    22561 atacgtatgg agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacaat
    22621 gatcatcggt tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat
    22681 tgtttgatgc tcaaagtcaa ggatagcaac aacaaattct gcatttgtca gttcttccat
    22741 atctttttcg tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg
    22801 gtgatgacgt ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat
    22861 acgacttact cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact
    22921 cattattttg cctccttcaa taataactga tttttaatag atactgctcg taattcattg
    22981 accagttcca atggattgtt ggaaaagatt acttgatcca accatttttt atccataaaa
    23041 tcctcttttg caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc
    23101 aatattccga ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag aagttgtgag
    23161 aattcctcca atgtacctag acctccaggg aaaacgagga cagcatctcc cttttcaaat
    23221 aataatacct ttctttcatc gatcgtttct gtaggaataa atgtagtaac atcttttcca
    23281 ggcaattcag tttcaaaaag cccattcgga tagatacctg tgacttttcc gttattttcc
    23341 agtactgttt tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg
    23401 atttcttctt gtgccatgta ttttcctaac gtttgcgcaa taaatttgta actttcttta
    23461 ttaccaaaac gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa
    23521 aagtctatct tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt
    23581 tagtggtata tagg
//
LOCUS       DANJJH010000039        23471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000039 DANJJH010000000
VERSION     DANJJH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(61..867)
                     /locus_tag="PWS53_001436"
     CDS             complement(61..867)
                     /locus_tag="PWS53_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871666.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            1005..2519
                     /locus_tag="PWS53_001437"
     CDS             1005..2519
                     /locus_tag="PWS53_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL0871667.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2922..3905)
                     /locus_tag="PWS53_001438"
     CDS             complement(2922..3905)
                     /locus_tag="PWS53_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL0871668.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3910..4557)
                     /locus_tag="PWS53_001439"
     CDS             complement(3910..4557)
                     /locus_tag="PWS53_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0871669.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4642..5274)
                     /locus_tag="PWS53_001440"
     CDS             complement(4642..5274)
                     /locus_tag="PWS53_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0871670.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5290..5841)
                     /gene="coaC"
                     /locus_tag="PWS53_001441"
     CDS             complement(5290..5841)
                     /gene="coaC"
                     /locus_tag="PWS53_001441"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL0871671.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5838..6575)
                     /locus_tag="PWS53_001442"
     CDS             complement(5838..6575)
                     /locus_tag="PWS53_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL0871672.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6572..7561)
                     /locus_tag="PWS53_001443"
     CDS             complement(6572..7561)
                     /locus_tag="PWS53_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0871673.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7819..8361
                     /locus_tag="PWS53_001444"
     CDS             7819..8361
                     /locus_tag="PWS53_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0871674.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8378..9463
                     /locus_tag="PWS53_001445"
     CDS             8378..9463
                     /locus_tag="PWS53_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871675.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9475..10281
                     /locus_tag="PWS53_001446"
     CDS             9475..10281
                     /locus_tag="PWS53_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871676.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10282..11109
                     /locus_tag="PWS53_001447"
     CDS             10282..11109
                     /locus_tag="PWS53_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871677.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11106..12179
                     /locus_tag="PWS53_001448"
     CDS             11106..12179
                     /locus_tag="PWS53_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0871678.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            12607..15303
                     /locus_tag="PWS53_001449"
     CDS             12607..15303
                     /locus_tag="PWS53_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0871679.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            15380..15868
                     /locus_tag="PWS53_001450"
     CDS             15380..15868
                     /locus_tag="PWS53_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871680.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            15880..16374
                     /locus_tag="PWS53_001451"
     CDS             15880..16374
                     /locus_tag="PWS53_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0871681.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            16395..17204
                     /locus_tag="PWS53_001452"
     CDS             16395..17204
                     /locus_tag="PWS53_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0871682.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            17201..18022
                     /locus_tag="PWS53_001453"
     CDS             17201..18022
                     /locus_tag="PWS53_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0871683.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            18073..20172
                     /locus_tag="PWS53_001454"
     CDS             18073..20172
                     /locus_tag="PWS53_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL0871684.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            20169..21287
                     /locus_tag="PWS53_001455"
     CDS             20169..21287
                     /locus_tag="PWS53_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL0871685.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            21326..22204
                     /locus_tag="PWS53_001456"
     CDS             21326..22204
                     /locus_tag="PWS53_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0871686.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            22395..22547
                     /locus_tag="PWS53_001457"
     CDS             22395..22547
                     /locus_tag="PWS53_001457"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0871687.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         7307 a         4031 c         4998 g         7135 t
ORIGIN      
        1 aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt ttagcatgtt
       61 ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt ggtaatcgtt
      121 tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat taacaaataa
      181 atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga ttgttttatc
      241 tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg gaacatgata
      301 gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga tcgtatcttt
      361 ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc acataacacc
      421 aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca agttcatggt
      481 ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag ctaaaaatcc
      541 gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat cacttaactc
      601 tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca tatttggaat
      661 ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta atgtcagtaa
      721 taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca taataacgac
      781 gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt ttgtactgtt
      841 cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc cttaattata
      901 ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt tgatattgtg
      961 aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa aaattagttg
     1021 ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca gatcagaaaa
     1081 atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca ggcgcttctg
     1141 aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg attattggag
     1201 ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg aatccagtaa
     1261 ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc ggtatgaatg
     1321 cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat gccttctatg
     1381 aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga tactttgtcg
     1441 atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg gacaacttaa
     1501 cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc tcggctattg
     1561 gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc cctatttttg
     1621 tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa gtgactgtac
     1681 atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt accacaggcg
     1741 gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct gattatatta
     1801 cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa ttaggtggac
     1861 aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat gagggggtct
     1921 tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag gggaaaagat
     1981 ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca ttgcctgaaa
     2041 aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct attgaatttt
     2101 atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca gaaaaaatcg
     2161 atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat gttgcggaga
     2221 aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg tctccgaatt
     2281 attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg aaaatcaata
     2341 cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat gctgctggtg
     2401 aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt gcggagatca
     2461 ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa aatgaatgaa
     2521 atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac cgagggtcag
     2581 ctgattgaac actgtttagt gaagaggttg tgaaaaagct gccctgcatc aagtaataag
     2641 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ttggacttat taccgcagat
     2701 gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta aagaacactt
     2761 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agaaattcct tcggaaataa
     2821 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
     2881 taaacgcttc tgtcccagcc tcttttttga tcagcatgat tttattcagt tatcttcaaa
     2941 atgatttttc cgatgttttt gtttgcttcc atatattcat gcgcttgttt gactgcgctg
     3001 aattggaatg tacggtcaat gattggccga atcttgccat tttctagaaa aggcccagct
     3061 ttttgcataa aagtttgtgc gagctctgct ttatactgat cacttctagg tgtcaataat
     3121 gtaccaagca gttggacacg tttcccaatc aatacaccaa ggtctatctc aggcacgatg
     3181 gtcccaccta gcataccaat caagacccaa cgtccatcaa cggctataga agctaaattc
     3241 ttttcccagt aagaagcgcc gatgaaatcc aaaataacat cgactccccg accttcagtg
     3301 atttttcgta cctcttcttg aaagtcttgt tttttatagt tgatcaattc atctgcacca
     3361 agttctttac aaaaggcgag tttttcttct gatcctgcag taacgatgat tttcgcgtga
     3421 gaaagctgtc tagctaattg gatcgctgct gttcccacac cacttgctcc tgcatggatc
     3481 aagacagttt cgccttcttg taattttcca agccagtaaa gtgtttgata agcagtcaaa
     3541 aaaacttctg agattccagc agcagaaaca tagtctagcg tatcagggag aaggattgca
     3601 cgatcggcag gcatcacagc atattcggca taaccaccta gattgactaa tccgtatact
     3661 tttgtcccag gagtaaaagt ggagatatca cttttattct caactacctc tccagctact
     3721 tcgactccca aaatagggta gggaggtttc aaagagactg attctctttt cataatatct
     3781 gttcgattga cagctgctgt atggactttg atcagtaatt cgccttgttt tggtttagga
     3841 acaggcactt caatttgttc taatacatct acagaaccag gatgcttgat tgaaatggca
     3901 cgcatggatc tattcctcct ttgcccattc ttggaatgct gtattcaact ctttgctacc
     3961 ttcagaaagt actagagtcg tacgagaaat atccgtttct tccactagag cggcaagtac
     4021 agcagcaact gtagagcgag tgatcgtttt tgatgccttc tcattaggct tagttaattg
     4081 aatggaagta acttcttcat cgttagtcaa tgtgactggt tgaagaatga caaaatctaa
     4141 gttagttcgt ttcagccatt catctgcata atgtttagta atataataat ctgtcatgga
     4201 ctcaggccat tttgctggtt catctgcata aacagcactg accatcagat agcgagagac
     4261 atgcgcattt tcagctgcga tcactgtttt gattgcgcca tccaagtcta cttgcagaag
     4321 attctttcct tgagagccgg cagcaaaaat caatacatcg atgcctttga atgcttcggt
     4381 cattttttcc acggaccaag ttaaatcaaa agagacatac gaaacattct ctttttttac
     4441 taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac ttggagctaa
     4501 atcttttatc aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa ttttcatttg
     4561 gatcatcctt tcgacgtcaa atctttccat tcaatagtaa caccaaaaga gaaaaaaatc
     4621 agagatcggc tacgatgact atcagttagt aagagaaggg tgcttgaagt atcttaacaa
     4681 gacattgcat actgctgcag tcgcaagacc tgcgacgatt ccttcaggta taccacttgt
     4741 aaaaataacc gctagaatag ctgaataaac agcatttaaa tttgttccga tgactttggc
     4801 ataatcttgc tggaataaaa agtagatcat attcatcact aaaatcgtat tggtcaatga
     4861 accggaaagt ccagctaaga ataaagaaac ggaatctgct ttttgtttca ttagtttgcg
     4921 cagaccttta taaacgaaat aaggaaccac cccaatcaag atgcgaggaa taaacgcgat
     4981 cagcaaggct ttccagcttc catgttctgt tccgatgact gggataaacg gagaaaagac
     5041 aaaagacaaa ggtgtttgga taactgtgct acggatcatg ctgatcagtc caaatgtacc
     5101 gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta caggaatatg
     5161 catggttgtt gcattgattg gaccgatagg gataaaacct aaaggcgtaa cagctaataa
     5221 aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt tcataagtac
     5281 tccttttttc taagcatttc tttcattaag agtcagcatg acctgttcga tgatcgtctg
     5341 aatatcagct aaggcacctt ttccaaaatc tccacaagct aaaagagatt ctcttggttc
     5401 aatttcctga tagccgacag atctcaatgt ttccagatta cgctgagtga tcgggttttg
     5461 atacatattc gtgttcatgg caggggcaat caattttggt gttcccactg gaagcgccat
     5521 tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct agcttgccga taatgtttgc
     5581 tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct agttcgatat gattgattac
     5641 ctgcggattt gattcttgca tgacgtctgt atggactgcg cgcttcgaca gagattgcaa
     5701 ggttaatggc gtgataaaat gtgtgctgct tttagacata atcacatcta cctgggcacc
     5761 taatttgacg aattgacttg taagatcagc gcttttgtaa gcagaaatac ttccagtaac
     5821 gcctagtaag atctttttca ttttgattcc tccagtctaa ttttctcagt aatcaatgca
     5881 gcaatctcag atttactttg agcttcttcc actgttccat ctttagataa gagatagccg
     5941 tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga aaataaaatc agttttgtta
     6001 tttatcagac tattttttgc gactgccaga agagattcct ttggtacatc tactaatagt
     6061 ttgaaaccaa tgatttttgc ttttggctgc cattcacgta gataagaaat gatttttggt
     6121 gttttcttca aaacgagcac aagatgctcg gtgttagaag agatctttga aacagtggta
     6181 tctgtctgtt cattttccgc aaaccaattg tccagttctt cttgcgttga agaagtgggt
     6241 aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc actgactgcc
     6301 atactgtgga taatcgcatc gtagtgttct tttttcgtta gttcttctaa ttgtaaaaat
     6361 aagtccagtg cagattcgat cgtataaatc gttatattgg agtgtctttt cggtttcagt
     6421 gcttgtttcg tagtcacgta atcgattgtg gtatccggat tggcagctaa ataatcagct
     6481 aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg atcgattttt
     6541 tcagtcgttc ctccagctgt gattagaatc ttcatttatg gtcacctaca tttactagtg
     6601 ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat tgatccagag
     6661 taatctcttg gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt tgccataaag
     6721 aaagacgttg atctattggt acattgacag aatcaattcc aatcaactgg atgtttcgca
     6781 gaataaaagg caagacagtc gcgtttagtt tgattccccc agcattgcca cataaagcta
     6841 ctgcaccgtt ataagagatc aatggaagaa gactgcttag ctgctcgcca cctactgtgt
     6901 cgatgacata gtcgatttgc tgttttccta aagcttttgt tgtctctggc agaaattcat
     6961 caggtcgtac gacttgcaaa gcacctagac tttttagcca tgtgacagac tcttcttttc
     7021 tggaaaaggc aatgattgac tggaatccta gtttatgtaa cagggctatc gctgtactgc
     7081 ctacaccgcc agaagcacca gtaacgacaa cttttttatc ggaggtcata ccttgacgga
     7141 ttaaagcgtt cacacacaac atagcagtaa accctgctgt accaaggatc atcgcttgac
     7201 gcagcgtcat attttttggt agaggaacta accagtctcc tggtactttt tgatattggc
     7261 tataaccgcc tggatgggtg acgccaagcc caaaaccagt agcaagtaca agatcttctt
     7321 ttgaaaaacg tggattgtta ctttggacga cagttccgga taaatcaatc cctggagtca
     7381 tcggatatgt tcgtatcacc tgaccagact cgctcattgc caatgcatct ttataattga
     7441 catcagaata ggcaattttt accaagactt cattttcagc caactcagta aatggcagat
     7501 cactgattgc tgtatggaaa acaggttcct ttattacctg aaaagatcga aaagcgttca
     7561 ttcgtgagac actcctttta aaattttctt ttatcatacc ataaagccta ctcttttatt
     7621 cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca aacaactaga
     7681 atttattcca aaagataaag aaatattcag caaaaaaaat tttacaaagg gttgcctttt
     7741 tcaataaatt tcgtatacta atacgagtgg tagtaaacag gaagtttact attagaaaac
     7801 ttcgattcgg aggaaattat ggaaattggt gagaagttaa gaaatttgcg catccaaaag
     7861 aatttgacac aagaagaact aggcgaacgc acagatttgt caaaagggta tatttcccag
     7921 ttggaacgag atttaagttc accatcaatg gaaacattct tttctattct tgaagtctta
     7981 ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga atctgcaagt ggtttattca
     8041 aaagaagatc atacggttta tttcgatgaa gaaaacgggt atgaattgga atggctcgtg
     8101 gctgattcga atgaaaaaga aatggagcct gtcttgctga cttttgaagg tgcaggagaa
     8161 tataagacgt ttgaaccttc attatctgaa acgtttattt ttgtattaga aggggaatta
     8221 gagttaagct taggagagac ggtctatgtg gcaaaagcag gacagtcgat ctattatcaa
     8281 gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt attgattgtt
     8341 gcaacgcaat catatttgta atataggggg taaaaacgtg gggaaaccaa tcatttcatt
     8401 tgatcaagta gtgaaacgct atgatgatga aacagtatta aaaaaagtaa gttttgagat
     8461 cgaacaagga aaattttata cactgcttgg tccttccggc tgtggtaaaa caacgatcct
     8521 acgtattata gctggattca ccgaagctac agaaggggac atctattttg aagggaaacg
     8581 gttgaatgat ttgccagcta ataagcgaca agtaaataca gtcttccaag attatgcact
     8641 ttttcctcat atgaatgttt ttgataacgt tgcttttgga ttgaagatca agaaaatgtc
     8701 caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc ttcctggata
     8761 cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag cgattgcacg
     8821 agcaattgta aatgaaccga aagtattgct attagatgaa ccgttatcgg cgcttgattt
     8881 gaaactacgt acagatatgc agtatgaact gcgggatctg caacaacgat taggaatcac
     8941 gtttattttt gttactcatg accaagaaga agcgttggca atgagtgatg agatttttgt
     9001 catgaataaa gggaaaatcg tccaaagtgg tacacccgta gatatttatg atgagccaat
     9061 caatcatttc gttgctgatt ttgttgggga gagcaatatc gtggacggtg taatgatcga
     9121 agataatcta gttgagtttg ttggtaagca atttgaatgt gtcgatggcg ggatgcgtct
     9181 aaatgaacca gttgaagttg ttttacgccc agaggatttg acgatcacga caccagataa
     9241 aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt atgaaatcat
     9301 ctgttatgat gaacagcaaa atgaatggat ggtccattca acgaaaaaag ctaaagaagg
     9361 aagcaaagta ggattggcct ttgagccaga agatatccat gtcatgcgtt tcaacgagtc
     9421 tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa taggggggac aagaatgaaa
     9481 acaatgcgca ggatctattc cgtcccttat attttgtggc tcggtttatt cgtgatcgcg
     9541 cctgtattaa tgatcattta tcaatctttt tttgatatga atgggcaatt cacgctgaat
     9601 aattatctat cttattttac gtcggggaca tcgctttcta tgacgatcaa ttctgtatgg
     9661 tatgcatttt tgatcacgtt tttcacgctg atcatcagtt acccgactgc ttatttttta
     9721 acaaaactga agcacaagca attatggctg atgctgatta ttttgcctac atgggtgaat
     9781 ctgctgctta aagcttacgc atttattggg attttcagtg tgcacggcag cgtgaatcat
     9841 ttctttgatt ttatcgggat cgggccgaaa cagatattat tcacagactt cagttttctg
     9901 ttcgtggcaa cgtatatcga gattccattt atgattatgc caattttcaa tgcattggaa
     9961 gagatgaatc cttctttgat cagtgctagt agagatttag gcgcgaatag ctttgagacc
    10021 ttccgacgag tgatttttcc gttatcatta aatggtgtga aaagtggtgt ccaagcagta
    10081 tttattcctt ctctttcttt attcatgctg actcgtctga ttggcggaaa tcgggtgatc
    10141 actttaggaa cggctatcga acagcatttc ttggttacac aaaactgggg gatgggttct
    10201 acaatcggtg tgattttaat cattgctatg ttcatcgtga tgctcctgac aggcgagaag
    10261 aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta tttagggatc
    10321 gtctttttat tcttatatgc accgatcttc tatctgattt tttattcctt caatgaagga
    10381 ggaacgatga acagctttac aggatttact tgggaaaatt atgaagctgt ttttgaagat
    10441 acacgtttga tcattatcgt attgaatacg ttcatgctgg ctttcttatc tgcattgatc
    10501 gccacgatca tcgggacatt tggggctatg ggaatctatt acacgaaaaa acgacgtgca
    10561 cggacagcgt tgttgagcat gaataacatc ttattagtct ctcctgatgt tattatcggt
    10621 gctagtttct tgattttctt tacactacta ggattcggat taggttttca gagtgtattg
    10681 ctttcccata ttgctttcag cattccaatc gtcgtcttga tggtcttgcc aaaattgcag
    10741 gaaatgaacg attcaatggt cgatgcggct agagaccttg gtgctaataa tttccaagta
    10801 gtcaaaaata tcattttgcc ttatctttct ccaggaatca tagctggata ttttatggca
    10861 tttacctatt ctcttgatga ttttgcggtt acatttttcg tcacaggaaa tggctttacg
    10921 acgctctcag tagagatcta ttctcgtgca agacaaggaa tcagtttaga aatcaatgcg
    10981 ctaagtgcgt tagttttctt gttctccatg atattggtca ttggatacta ctttattagt
    11041 aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg ggtggcacgg
    11101 ctgcgatgaa aaaattacaa tctctgtttt taggtattct catcattatt gcggtgctgc
    11161 tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag gtacttacca
    11221 tctataactg gggagattat atcgatccta gtttgatcac gaaatttgaa aaagaatatg
    11281 gatacaaagt gaattatgaa acatttgatt ctaatgaagc gatgttcaca aaaatccagc
    11341 aaggtgggac aaactacgac atcgctatac caagtgaata tatgatccaa aaaatgatca
    11401 aagaaaaact agtacttcca ttagatcatt cgaagatcaa aggattaaat aatattgatc
    11461 cacgattttt agacttggat tttgatccag ataatcagta ttcgattcca tatttttggg
    11521 gaactttagg gatcgtatat aacgataaat tctttgatgc aaatcaaatt aagcattggg
    11581 atgatttgtg gaagccggaa ttaaaagaca gtttgatgct gattgacggt gcacgagaag
    11641 ttatggggct ttctttgaat agtttaggct attctttgaa tagtaagaat atggcacagt
    11701 tgacagaagc agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca atcgtggcag
    11761 atgagatcaa aatgtatatg atcaatgaag aagcctccgt ggcagtgaca ttttctggcg
    11821 aagctgccga tatgatgtgg gaaaatgaac atttacatta tgtgatccca ccagaaggat
    11881 ccaatctttg gtttgataat atcgttatgc caaaaaccgt gaaaaataaa gaaggtgcct
    11941 atgatttcat caactttatg ctagaaccgg aaaatgcggc acaaaatgca gagtatattg
    12001 gttattctac accaaatgaa gaagcaaaag cgttattgcc aaaagacatt tcgtcggacg
    12061 aacaattcta tccatctgat gacacgatct cccatcttga agtctacaaa gatctaggat
    12121 cgaaatacct aggcatctat aatgacctat tcttagaatt caagatgtat aggagataat
    12181 ggcaaaactc agtaagaatg gggacttatc aacgttctaa agagctaaaa atcgtccttt
    12241 ttctatctga gttttccaat cttctaccaa aagcggatga aacgccgctt tttttgtgtt
    12301 attttatttt ttctaccacg tttttctacc agagaaaagt ggacaaaacc attgtaaaat
    12361 agggcccgag acgggctagt aaatctaaga ttgaaaaagt tacagaagga taaagtaatt
    12421 ggaaactatg caaatatact taacccttct gtaatctatt aataaaacta atatatgaat
    12481 ccagttcaat atttgtagct atgaaaaaac ataaaaatca tattagataa aaactaaatt
    12541 ggtagataaa ttattttgtc agatatctga ggcaaatatt cgactatttg tgtttcaaag
    12601 taaaaattgg aatacaaata tagaatagac atattgcagc cgatagttta ttatgctttt
    12661 agacacatta aactggagga aaaaatgaaa aagagcgcta aagatcgagt ttttgatgag
    12721 ctgatacgac taacagggaa tatgtcttct gtcactgccg agaaaatttc gaagaatcta
    12781 ggtatctctc gtcagaatgc cagccattat ttgacgagat tagtggaaga taaaaaggtc
    12841 gagaaattac cgggaaagcc tgttttgtgg aagcctttag atgaatatgc ggttgtagat
    12901 aatacggagc aaataaacga agcctttcat tctgtagttg gtcatgacgg aagtctgaga
    12961 gaggtcattc aaaaatgtat agccgctgtc aaatatccgc ctaacggact cagtgtatta
    13021 ataaatggag ccacgggtgt tggaaaaagt tttttagcca caaaaatttt tgaatacgct
    13081 atccatgagc aaattattga aaaagatgca ccatttgcta tcttaaactg tgccgattat
    13141 gcggacaatc ccgaactgct ttcggcaaca ctgtttggat acaagaaggg ggcctttacg
    13201 ggggctgaaa aggataccga tggtttattg gcaacggcca acaacggcta tctttttctc
    13261 gatgaggtcc atcgcctatc gaaagaaaat caagaaaagc tctttctatt tattgatact
    13321 ggaaactatc gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt tcgttttatt
    13381 ttcgccacga cggaaaaagg tgaaaattat ttattagaca cctttgatcg gcgtattcaa
    13441 attagtgtta tgttgccaac ttttgaagat cgccccattc gagaacgatt agagctgatt
    13501 caattatttt ttcaaaatga agcggatgtt ttacaaaaag atattattgt ttcaagagaa
    13561 gccttgtctt taatgttatc tcatcccttt agtgggaata ttggtaagct gaagaatatc
    13621 attaaaatca gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt aacgattggg
    13681 aaaaatgaga tcttgattca gttaaatatg ctggaatccg aagtagaaag cttgccgatt
    13741 gctagtttat tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa accgtttgat
    13801 tatgattcga ttttggaaaa aattactcag ctgataaatg aaggtaattt caatgaaaat
    13861 ttttcttcga taaagaatga agtacagcat ttggtgcatc acattgataa ggattctttt
    13921 ttatcaatga gtagaaaatt gatgattcag atgtttagaa aggtctgggt atccgtttta
    13981 ggaaaaacgt acggattgac gacgagtgga cctgttgcag aaattattag cagatcctat
    14041 gtcatttccc caagctataa gacagactta accgggataa atcgtacatt agctgcaaat
    14101 ctacctcgga cgacttactt atgtaatcag ttcttggagc atttaccacc gctctcagaa
    14161 aaggacgaga actttctaag actgcttatt gtcgtgactt tatctgatta tgtagatgaa
    14221 tccatcgagc tgaagggcct gttgcttgct cacggagata gtacggctag cagcattcaa
    14281 gcagtagtga atcaattatg cggcaattat gtttttgaag cgttggatat gccagtagag
    14341 agtaatgtgt cagagattgt cgaatcgaca aggaatttcg tgaaacggca agtgaataca
    14401 gatagtctag tgctgatcgt agatatgggc tctttaaatc aaatttacac acagatcaag
    14461 aacgatttaa aaggggagtt acttcttctg aataatttga cgacttccat tgcacttgat
    14521 attggcttga agatgacgat gaatgcgcca tttaaacaga ttgcggagga tgcagcagac
    14581 aagtattcaa tcaatgttca atactttgaa ggcttttctc aaagtagcaa cattatcatt
    14641 tcctgtatgt cgggtttggg gatttcggac aaattgaaag atgtttttcg tcatcattta
    14701 tcggatgaat ccatcgaagt tttcacgaag gactacagag aactacgtga attaatcgac
    14761 caaaatgatg aaacttattt tgacaagaca aaattggtca tcacgacttc ggatcttcca
    14821 cgttcctttt caattcctaa tattaatgtt tatgacatct tagacggtga tggagcagcc
    14881 aatttggagc atattttgag tcctgaaatg aagccggcag aatttaaccg cttgattcag
    14941 gagctagtaa aattcttttc gttggaagga attgcggacc gtttgaactt tctgaaccca
    15001 acgatcgtga tgagtgaagt ggaaacagtc atttctaaat atgaaaatta ctatcatttc
    15061 actttaaatg gccgtgtaaa actaaatctc tatatgcata cggcattgat gatggaacgt
    15121 ttattcttgg ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa accagatgaa
    15181 gatgaatttt attatgtttc tagaagtatt tttcaatcaa tggagatgaa atataatttt
    15241 aaagtcaatg cttatgagct ttcgctgctc tatgaattat taggaccgta tattcaaaaa
    15301 taaagtgtct aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt ttttaatgtg
    15361 aattaacgtg attaaaccaa tgtttttaaa agttggcaca ggaattgctt atataaaagt
    15421 gaacagaaag aaaggaatta accatatgaa gaaaattcta atagcgactc acggtcactt
    15481 agcttcaggc tttcttagca gtattcaatt actgacgggg aaggttcaag agattacggt
    15541 gattaatgct tatgtggatg agtgtgattt tgaacaggag ttgaatgtat ttgtaaacca
    15601 ggtgaacgag caggatcaga tttttgtttt taccgattta tttggcggaa gtgtcaatca
    15661 gaagattacc aaaacctttt tggagaaaga gattgctgta actattttgt ctggctttaa
    15721 tctgccaatt ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg aggatattca
    15781 ggaactggta aataaatgcc aacaggaatt aaagatgacc attcttgatg aggtacatgt
    15841 agtaaatgaa gaggaagatt ttttttagga ggaagaaaaa tgattaaagc agtgcgaatt
    15901 gatgaacgat taattcatgg acaagtagcg atgacttgga caaagagtct aaatttaacc
    15961 gggttagtag tagcaagcga tgaggcggct accaacgata ttcaaaagat gacattgaag
    16021 atggctgcac catcgaatgt caaagtcatt attaaaacag ttgatgacgc gattcatttg
    16081 ttaaaagatc cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa tgtcaaagat
    16141 gcggtaaaag ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt ggggaatgct
    16201 gggaaaatga caccgccatc ggaagataag aaaaccttat caaaggaagt gatgttaacg
    16261 acgtcagaaa ttcaagactt aaaagaatta gttgaacttt atccagatac cgtttttcag
    16321 cctacaccgg cgatggaaaa acgttcagct atctctattt tgagtgattt ttagaaaaag
    16381 gaaggagaaa aattatgtat gatgcatttg tagtcgcttt ggccgttttt attggaattg
    16441 ctggacatga aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt gctccgttag
    16501 caggagcgtt gatgggggat ttgcagacgg ggttgattgt cggggcgtca ctagaagcca
    16561 tctttatggg tgtcgtcaat atcggaatct ctagtacggc ggagccttct ttggctgctg
    16621 ggctggctac agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt attcccattg
    16681 tctttccact agcagtatta ggattacagt taatgaatat gattttttct tttgtatgtg
    16741 gaccgatggc tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa ggaatcatca
    16801 atctgcactt ctttatgtgg tttgtccact atgcgcttta tgctcttatt ccattctttg
    16861 ccgttttatt tggagcagat gttgtcaaaa gcgtcttgga taatattcca gaggtcatca
    16921 tgaacggatt aacagtcgct ggaaatctgc tgccggcagt tgggatggcg atgctgttac
    16981 ggatgctttg gagtaaaaat attgctgtgt ggtttttctt tggggccgtt gttatggcgt
    17041 atctcgagtt accattgatt gcaattgccg cagttggaac gattattgca attgttatag
    17101 cccaaagaga tctacaacta aaaaatatat ctgttcatgc agttcaaagc gatgctgctg
    17161 taaatgcaac gcttgaccaa gaagaggagg atttctttca atgaaattag ctgatggaat
    17221 ttctaaagat gagaaaaaat tggttcggaa gatgttttgg cgctcgatga ctatgtatgc
    17281 ttcggtgaat ccggtgacga tgggcggcgg tggtttcgcc tattctatga tgcccttcat
    17341 taatcatttc tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc atacacagta
    17401 tttttcgacg accattcctt gcgcgtcctt tgtcatgggg attgcggcat cgatggagaa
    17461 ggaaaattct gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca aaacgagttt
    17521 gatgggacca ttggctggaa ttggcgattc attgttctgg ggagtttggc gtgtcgttac
    17581 cgcgggacta gcgatcaact tagccaatgc cggaaatatt ttggcaccaa ttatcttttt
    17641 agttatgttc aatataccta attacttgat gcgatactat ggcggcgttt taggctattc
    17701 attaggctct aaatatattg aaaagctgta tgcgaacggg ttaattgaaa ttctgacaaa
    17761 agctgctagt atcttgggtc tgatcatggt aggtgcaatg acgatcagta tggtcaaatt
    17821 caataccgtt ttgagctttt caatgaaggg cgaagaacta atgaatgtcc aagaaatcct
    17881 cgatagtatt tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt tcaaattact
    17941 agataaaaaa gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg gaattttatt
    18001 gtcattactt ggaatcgcat agtaggaagc tgcagctact aggctgcagt ttttttagca
    18061 aggagggaaa agatgactat taatgaagta atttattcac ccaaacaccc attttttaat
    18121 gctgagttaa acgacggaaa tgtgactact tcaaacgaat acagctacta tcggaatatt
    18181 cctgatgtaa agggattgac cttcgatgta agtaatcaaa agacattagc aaaccttgat
    18241 gtttgcggga cgattcaaac gttgaccttt tatcgagaaa atttactaac tgaggaaaaa
    18301 ccaggtgttt gggtcaataa acagctctct caaaccaatt cgttatcctt aaaggttgaa
    18361 gtaaacggga ttgtctacca tttgtcagaa tcggatcatc gtattgaagt cgatgtgatt
    18421 caagactgtt tgcctagata tattcatcat tattcgacgt ttaaggtcat tgtagtatcc
    18481 tttgctccca tactggctaa taagcgttta agtatgcttg ttcaacaggt ttacattgtg
    18541 aatgaaacac acgaaagcct tcaagtttca gtgaaagatg tgccttcgta tcaagagaag
    18601 tattcggatc agcaaaatgt gttgatttct caaaaaggaa ataaggacac gatcgcacaa
    18661 caggaagtgg caagcttcgc cattgctttt gtggacccaa atgcttttga agaagtaatg
    18721 acttttcagg aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta ttttgctcag
    18781 ctatttgggc agctgtcaat gccaaacgat agagttgtcc atctctatcg tcgtgcactt
    18841 tatcaaagct tttctagttt cggtatgaat cgacagggac aggtcgtcgg cagcaattgg
    18901 ggcagctatc ctgctactaa tcgaatttgg aataaggata tgtattacgc atcattgcca
    18961 ttcaccatgt ttgaaccgga gttatgtcaa aaaacggttc tatggtttga tcaatatggc
    19021 gttaagtttc ctggttcaaa atttacagga ggtatcaatc attctctgag taattcgtta
    19081 tccagtgcgt tactggctag tctttatttt gaacatactg gggatacaaa attctttgag
    19141 cggtgtccgc aggttctact gaatgcttca agaattattg aggacatatt agctcaacgt
    19201 cggtcagggg aaccaatgct ttttccatct gtttggttat cagacgcctt tgcacttgga
    19261 aagtatcata ccggcagcaa tctttgtctt tggaaggcct gtattggact tgcagaaatt
    19321 tttgaagccc tagaacaact gagtcttagc aagcgataca aaaacattgc ctgtaaacta
    19381 aaggaatcaa tttgtaaaca aatgactatc gatggtcctt ttggtgagca atttttggag
    19441 ggaatcggtg atgaggagaa aacaacctat tcagtgaagc actatcaaaa accgattctt
    19501 gagcaagggt taatcttttt aagtgatgtg attgtggatg gaaaaattaa tttattgatg
    19561 catgatggag aagaatcaga cactacgttg atgccttttt atcaattttt agataataaa
    19621 gatcatttat atcagaacac catggccttt tctgctagtt ctttcaatcc aacctatagc
    19681 gaggaaatca aaggcatcac ttggggatta gaatccggcg ccacttttcc tggctttatc
    19741 actgttttaa tgagcgagct gcacaaccct caagctttcg taacccgatt agaagaacta
    19801 atttgtttgg cagatttaga cggttcgtgg tggtggtggc cgtatcgctt agaggctcaa
    19861 caaggagaag tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc gggtcttttt
    19921 gtcagcctaa tgatcagtca atatttcgga ttgaaattca aaaaaggagt attgcaaatt
    19981 gatcctgttg ataatttaac gttttcttgg aagaatttga agttcggtca agccttcatt
    20041 tcgattgaat gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa gccattgaaa
    20101 ataagtgata cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca cattcaaagg
    20161 agaaaaagat gagtatacta aaaaatatta cccaatttaa aaaaatccag agtcgagcaa
    20221 tcactcccga aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca agcgagctag
    20281 gaccaagccg caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa actgtgattg
    20341 cggatattac tggaacggga ataattcgtc atatgtggat gacgattcgt gatcgaacag
    20401 aatttggcag ttttgtttta cgtgatttaa tcttaagaat ttattgggat gggtcggata
    20461 gccctgctgt tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga gagcgttgcg
    20521 aggtgaactc gttgccgata gtggttaatc ctacaggggg ctttaattcc tatttcgaaa
    20581 tgcctttcca aacatccgct aaaattacga tcgagaattt acatccgaag gatatgtcct
    20641 cattttttta tacgataaat tacgctttgg tggacgaatt ggaagaaaat agcctatatt
    20701 ttcacgcaaa ttggcatcgg gaacgcatta ccaaattaca ggaggattat aagttaattg
    20761 atcgattaaa gggtcaaggc tattacgtgg gtacttacct tgcgcttacc tgcctagaac
    20821 gttattggtg gggcgaaggc gagtttaaat tctacattga tggggatagt gagtatccga
    20881 ccgtaacctc aacaggttca gaagattatt ttggcggggc gtgggccttt catgagtacg
    20941 atgagttcgg acgtccacat gcgaaaaact attcaacagc ctttttaggg tatccttacc
    21001 aatcaaatag agatgccaca agagatcgtt ttgaaacggg aaaattgaat gccgttcatg
    21061 cctttggtga cgacgcaatg ccacgacatg gtctttatcg ttggcatctt caagacccca
    21121 tctgttttac cgaagattta tcggtcactt tacaacaaat cggcaacgat gatgttcgct
    21181 tatttgagcg gcaagacgat gttgctagtg tcgcttactg gtattcaaca gaagcttcag
    21241 gaaatactaa acgtcatttc actgatcgag aacgcttgcc gcgttaatga agtgtttttc
    21301 acatattcga agggaggaac ctatgatgat atatggggca attgaagctg gagggactaa
    21361 atttgtctgt gcagtcggta ctgaggaatt aacgattttg gaaagagtca gttttcccac
    21421 aacaactcca gttgaaacaa tgaagcaggt cattgatttt tttaaacctt ttaaggaaca
    21481 attggcaggc atcggggtcg gctgctttgg tccaattgat attcaatgca aatcaacgag
    21541 tttcggtcat atcacatcaa caccaaaact tgcttggcaa aatttcaatt ttgtaggaac
    21601 gctaaagcaa tatttcaata ttccgattga atggacaaca gatgtcaatg cggcttgcta
    21661 tggtgaatat gttgctggtt ctggcaaagg gttagcaggt gttgcatact tcactgtggg
    21721 aacaggtatc ggaggtggtg ccttgttcaa tgggactttc gtggaagggt tcagtcatcc
    21781 ggaaatgggg catatgttga tcaagcgaca tccaaaggac aatttttcag gaaactgtcc
    21841 cttccaccaa gattgtttag aggggatggc agcaggacca gcgattgaaa aacggttggg
    21901 tgtaaaaggc cagaatttat tggctgacga ttcgttttgg cagattgaag ccttttacct
    21961 tgcccaatgt gcatacaata caacactgat gttttcgcca gatagaatta tttttggcgg
    22021 tggtgtaatg aaacaagaac acatgaagaa aaaagttcag gataaatttg ttgagcttat
    22081 aaatggatat gttgaaatac caccaattga tagctacatt attacgcccg aattaggtga
    22141 taatgccgga ataatcggcg gattagcatt ggccagaaaa gcggtaagaa ataagcaacc
    22201 ataattaatg aatgtttcat tgatactcat gtcaaaagta tttaaccgag taatcaaatg
    22261 atcaaatgaa gttggataaa gcgaaaatga attcaaataa aaaggctcta agtcaaatcg
    22321 tgttgttttc attaaatcac tgaaaggaaa tgcttctgcg tttccttttt tctttttgtt
    22381 gtgcgctgta taccttgagg atattcactg gtggcgtaca cttaaatagt aatatattgt
    22441 gttactttat gtctgtggta atagttatag ttgcttacca atgtatgttc atttttcgaa
    22501 atgttgactt cataatcctt ggcttaatgt ttgaattttt ttggtgatag attttttcag
    22561 ccgtataaaa taaatcgtaa ttctattata gagagttgtg attataaaaa gatagcaaga
    22621 aaaaaaaaga aagtttctat acattttaaa tattttattc ctatttctat tgctaaaaaa
    22681 gtagaaattc tatatttacc tttaactctc gcttctcttg caaatgtagt tagcatttta
    22741 atcgataata aactcaggta gttatattac cttgaaatga ttgataattt tgtcacttct
    22801 attttttttg caataaattt tttattttag tgaaaaataa gaaactattt ctttgaaggc
    22861 gataagttag aaaaatatta ttgtagtctt ttctttatta ggaatcatca ctgttgtatc
    22921 ttattacctt ttctgttttt cttattagag aaaaataatt tgagaggtag atagttttta
    22981 ttggaggaag taattatgta tatttttgaa acaaaaattt ttcattgcaa taattgcaac
    23041 aaagaaattt catcggggga agaggtttgg acaaaaatgg aaatttccgc caaaagtaac
    23101 tgctcctcaa ctgaaatcaa ttaaagaact agaatttgag aatgcactgg taatttgtat
    23161 gacatgtact gatgaagtat ttcgaaaaca tttttaaagg ttgagatgta tatagattta
    23221 accaatcatt tatctctctc ggaaaaggaa tattaactaa ccagcgggtt atcaaaaata
    23281 ggtaggaaaa ctgtacttaa tggcttattt cgcgctgatt atgagaatat cagtctgttt
    23341 gattaatttt tagggttcaa aatacagaga caaactccag gtcgggtttc gccacaagcg
    23401 aattacccga tctggagttt tgtctattgc aggaaggcgt agccggactg caggaataaa
    23461 gcccccggtt c
//
LOCUS       DANJJH010000040        23064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000040 DANJJH010000000
VERSION     DANJJH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(110..565)
                     /locus_tag="PWS53_001458"
     CDS             complement(110..565)
                     /locus_tag="PWS53_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871688.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="PWS53_001459"
     CDS             complement(719..1927)
                     /locus_tag="PWS53_001459"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL0871689.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="PWS53_001460"
     CDS             complement(2039..2371)
                     /locus_tag="PWS53_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0871690.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="PWS53_001461"
     CDS             2519..3394
                     /locus_tag="PWS53_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0871691.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="PWS53_001462"
     CDS             complement(3476..4057)
                     /locus_tag="PWS53_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL0871692.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="PWS53_001463"
     CDS             complement(4063..5322)
                     /locus_tag="PWS53_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0871693.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="PWS53_001464"
     CDS             complement(5324..6370)
                     /locus_tag="PWS53_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871694.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="PWS53_001465"
     CDS             complement(6605..6880)
                     /locus_tag="PWS53_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL0871695.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWS53_001466"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="PWS53_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL0871696.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWS53_001467"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="PWS53_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL0871697.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="PWS53_001468"
     CDS             complement(9979..10659)
                     /locus_tag="PWS53_001468"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL0871698.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="PWS53_001469"
     CDS             complement(10708..11343)
                     /locus_tag="PWS53_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0871699.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="PWS53_001470"
     CDS             complement(11409..12812)
                     /locus_tag="PWS53_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL0871700.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="PWS53_001471"
     CDS             complement(12799..13830)
                     /locus_tag="PWS53_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871701.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="PWS53_001472"
     CDS             13861..14082
                     /locus_tag="PWS53_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL0871702.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="PWS53_001473"
     CDS             complement(14247..14984)
                     /locus_tag="PWS53_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL0871703.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="PWS53_001474"
     CDS             complement(15137..16477)
                     /locus_tag="PWS53_001474"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL0871704.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="PWS53_001475"
     CDS             complement(16714..18237)
                     /locus_tag="PWS53_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL0871705.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="PWS53_001476"
     CDS             complement(18615..19226)
                     /locus_tag="PWS53_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0871706.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="PWS53_001477"
     CDS             complement(19556..20272)
                     /locus_tag="PWS53_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0871707.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWS53_001478"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="PWS53_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL0871708.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="PWS53_001479"
     CDS             complement(20831..21628)
                     /locus_tag="PWS53_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL0871709.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="PWS53_001480"
     CDS             complement(21598..21969)
                     /locus_tag="PWS53_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0871710.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWS53_001481"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="PWS53_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL0871711.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DANJJH010000041        22031 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000041 DANJJH010000000
VERSION     DANJJH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            367..555
                     /locus_tag="PWS53_001482"
     CDS             367..555
                     /locus_tag="PWS53_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871712.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="PWS53_001483"
     CDS             545..805
                     /locus_tag="PWS53_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871713.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            1138..1710
                     /locus_tag="PWS53_001484"
     CDS             1138..1710
                     /locus_tag="PWS53_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871714.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="PWS53_001485"
     CDS             1711..2022
                     /locus_tag="PWS53_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0871715.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="PWS53_001486"
     CDS             2013..2273
                     /locus_tag="PWS53_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871716.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="PWS53_001487"
     CDS             2434..2793
                     /locus_tag="PWS53_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871717.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="PWS53_001488"
     CDS             2790..4433
                     /locus_tag="PWS53_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0871718.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="PWS53_001489"
     CDS             4468..5550
                     /locus_tag="PWS53_001489"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871719.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="PWS53_001490"
     CDS             5594..6766
                     /locus_tag="PWS53_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0871720.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="PWS53_001491"
     CDS             6753..7457
                     /locus_tag="PWS53_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0871721.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="PWS53_001492"
     CDS             7462..8622
                     /locus_tag="PWS53_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0871722.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="PWS53_001493"
     CDS             8698..8976
                     /locus_tag="PWS53_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0871723.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="PWS53_001494"
     CDS             8957..9328
                     /locus_tag="PWS53_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL0871724.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="PWS53_001495"
     CDS             9344..9748
                     /locus_tag="PWS53_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871725.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="PWS53_001496"
     CDS             9745..10155
                     /locus_tag="PWS53_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871726.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="PWS53_001497"
     CDS             10216..10803
                     /locus_tag="PWS53_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0871727.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="PWS53_001498"
     CDS             10876..11343
                     /locus_tag="PWS53_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HDL0871728.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="PWS53_001499"
     CDS             11389..11697
                     /locus_tag="PWS53_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871729.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="PWS53_001500"
     CDS             11892..18626
                     /locus_tag="PWS53_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0871730.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="PWS53_001501"
     CDS             18630..19502
                     /locus_tag="PWS53_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL0871731.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="PWS53_001502"
     CDS             19499..20548
                     /locus_tag="PWS53_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871732.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="PWS53_001503"
     CDS             20552..21376
                     /locus_tag="PWS53_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871733.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="PWS53_001504"
     CDS             21394..>22031
                     /locus_tag="PWS53_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL0871734.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DANJJH010000042        22026 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000042 DANJJH010000000
VERSION     DANJJH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(61..1371)
                     /locus_tag="PWS53_001505"
     CDS             complement(61..1371)
                     /locus_tag="PWS53_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0871735.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="PWS53_001506"
     CDS             complement(1383..2801)
                     /locus_tag="PWS53_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0871736.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="PWS53_001507"
     CDS             complement(3041..4609)
                     /locus_tag="PWS53_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0871737.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="PWS53_001508"
     CDS             complement(4613..4894)
                     /locus_tag="PWS53_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0871738.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="PWS53_001509"
     CDS             complement(4891..5814)
                     /locus_tag="PWS53_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL0871739.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="PWS53_001510"
     CDS             complement(5811..6689)
                     /locus_tag="PWS53_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL0871740.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="PWS53_001511"
     CDS             complement(6676..8013)
                     /locus_tag="PWS53_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0871741.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="PWS53_001512"
     CDS             complement(8016..9119)
                     /locus_tag="PWS53_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0871742.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="PWS53_001513"
     CDS             complement(9193..10701)
                     /locus_tag="PWS53_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0871743.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="PWS53_001514"
     CDS             complement(10667..11359)
                     /locus_tag="PWS53_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0871744.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="PWS53_001515"
     CDS             complement(11381..12355)
                     /locus_tag="PWS53_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0871745.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="PWS53_001516"
     CDS             12655..13527
                     /locus_tag="PWS53_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871746.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="PWS53_001517"
     CDS             complement(14109..16307)
                     /locus_tag="PWS53_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871747.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="PWS53_001518"
     CDS             16539..17531
                     /locus_tag="PWS53_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871748.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="PWS53_001519"
     CDS             17591..19012
                     /locus_tag="PWS53_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL0871749.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="PWS53_001520"
     CDS             19005..20504
                     /locus_tag="PWS53_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL0871750.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="PWS53_001521"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="PWS53_001521"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0871751.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DANJJH010000043        21939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000043 DANJJH010000000
VERSION     DANJJH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            4..1143
                     /locus_tag="PWS53_001522"
     CDS             4..1143
                     /locus_tag="PWS53_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL0871752.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1136..1936
                     /locus_tag="PWS53_001523"
     CDS             1136..1936
                     /locus_tag="PWS53_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0871753.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1936..2763
                     /locus_tag="PWS53_001524"
     CDS             1936..2763
                     /locus_tag="PWS53_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0871754.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2741..3475
                     /locus_tag="PWS53_001525"
     CDS             2741..3475
                     /locus_tag="PWS53_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0871755.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3575..4441
                     /gene="rfbA"
                     /locus_tag="PWS53_001526"
     CDS             3575..4441
                     /gene="rfbA"
                     /locus_tag="PWS53_001526"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL0871756.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4455..5027
                     /gene="rfbC"
                     /locus_tag="PWS53_001527"
     CDS             4455..5027
                     /gene="rfbC"
                     /locus_tag="PWS53_001527"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL0871757.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5049..6077
                     /gene="rfbB"
                     /locus_tag="PWS53_001528"
     CDS             5049..6077
                     /gene="rfbB"
                     /locus_tag="PWS53_001528"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL0871758.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6175..7026
                     /gene="rfbD"
                     /locus_tag="PWS53_001529"
     CDS             6175..7026
                     /gene="rfbD"
                     /locus_tag="PWS53_001529"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL0871759.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7061..9094
                     /locus_tag="PWS53_001530"
     CDS             7061..9094
                     /locus_tag="PWS53_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871760.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9138..10418
                     /locus_tag="PWS53_001531"
     CDS             9138..10418
                     /locus_tag="PWS53_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL0871761.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10628..11434
                     /locus_tag="PWS53_001532"
     CDS             10628..11434
                     /locus_tag="PWS53_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871762.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11446..12666
                     /locus_tag="PWS53_001533"
     CDS             11446..12666
                     /locus_tag="PWS53_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871763.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12656..14242
                     /locus_tag="PWS53_001534"
     CDS             12656..14242
                     /locus_tag="PWS53_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0871764.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14281..16419
                     /locus_tag="PWS53_001535"
     CDS             14281..16419
                     /locus_tag="PWS53_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0871765.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16787..17770)
                     /locus_tag="PWS53_001536"
     CDS             complement(16787..17770)
                     /locus_tag="PWS53_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL0871766.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18148..19539
                     /locus_tag="PWS53_001537"
     CDS             18148..19539
                     /locus_tag="PWS53_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0871767.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19555..20595
                     /locus_tag="PWS53_001538"
     CDS             19555..20595
                     /locus_tag="PWS53_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0871768.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20614..21699
                     /locus_tag="PWS53_001539"
     CDS             20614..21699
                     /locus_tag="PWS53_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL0871769.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21732..>21939
                     /locus_tag="PWS53_001540"
     CDS             21732..>21939
                     /locus_tag="PWS53_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0871770.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFS"
BASE COUNT         7097 a         3573 c         4493 g         6776 t
ORIGIN      
        1 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
       61 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
      121 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
      181 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
      241 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
      301 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
      361 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
      421 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
      481 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
      541 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
      601 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
      661 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
      721 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
      781 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
      841 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
      901 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
      961 ggaacgatcg tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     1021 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     1081 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     1141 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     1201 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     1261 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     1321 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     1381 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     1441 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     1501 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     1561 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     1621 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     1681 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     1741 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     1801 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     1861 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     1921 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     1981 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     2041 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     2101 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     2161 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     2221 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     2281 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     2341 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     2401 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     2461 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     2521 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     2581 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     2641 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     2701 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     2761 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     2821 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     2881 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     2941 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     3001 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     3061 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     3121 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     3181 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     3241 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     3301 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     3361 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     3421 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     3481 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     3541 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     3601 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     3661 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     3721 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     3781 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     3841 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     3901 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     3961 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     4021 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     4081 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     4141 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     4201 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     4261 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     4321 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     4381 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     4441 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     4501 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
     4561 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
     4621 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
     4681 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
     4741 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
     4801 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
     4861 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
     4921 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
     4981 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
     5041 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
     5101 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
     5161 atgcaggaaa caaagagaac ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag
     5221 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
     5281 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
     5341 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
     5401 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
     5461 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
     5521 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
     5581 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
     5641 ttattcctcg ccaagtaacg agtatcttaa gcggtattcg tccaaaatta tatggcgaag
     5701 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
     5761 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
     5821 agaccgtgat ggaattgatc ttagaaatga tgggccaacc aaaagacgca tacgaccatg
     5881 taaacgaccg cgcagggcac gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag
     5941 agctaggttg gcagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
     6001 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
     6061 ccaaaaatgg acaataatac agaggaaatt tcactgtatt aatggaaaaa cacatgtgag
     6121 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa ccatatgttt
     6181 ttattaactg gaggaaatgg acaattaggt acagaattgc gccatttatt agatgaaaaa
     6241 aatctgccat atgtttcaac agatgcaaaa gacttggata tcacagatgc tgagaaaaca
     6301 atggcttata ttacagaatt aaaacccgat gttattttcc attgcgcagc ttacacagca
     6361 gtagataaag cagaagaaga agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca
     6421 agaaatgtag cacttgcagc aaaagctgtg aatgcacaat tcgtctatat cagtacagac
     6481 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
     6541 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
     6601 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
     6661 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
     6721 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
     6781 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa
     6841 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
     6901 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaagc gttaggtttt
     6961 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
     7021 aagtaatttc ctttttatca aatagaattt ggagcatgat atggaaaaac aaacaagaag
     7081 tgcacgacga aaagaaaagc caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct
     7141 gtttctaaaa ctccgctatc ttattggcgt tttagtcatc gtggtcatcg ttacttttaa
     7201 tttaaacgga agttcacttg gtgtttggga taaatatgtg tcgcaaagag acgatgggaa
     7261 aaaaactgat gtgatttttg gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca
     7321 gacacctttt tacctctcac aagctgaaga ggacttttcg ttggtgaacg aggattattc
     7381 attaaacgga caaaacatga tcattgcgta taattctcct gtaaaggata ttacggttat
     7441 cggaaaacca ttcaactggg gattcttttt cctcggaaga gatcgaggac tttcctttta
     7501 ttgggcgatg aaattagtcg tcatggtctt gcttggattc gaagtactga tgatattaac
     7561 gaagaggaaa aaagctctat cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt
     7621 ccagtggtgg ttcatgcaac atgtgggcga tttgattttc tttactttgg gactgatgat
     7681 tgctttttat cattatttct acaatcatga taaaaaatgg ctacgaacgt tgatgatgct
     7741 tttggtggtt attttcggta ttgggtttat tttagtgatt tacccagccc atcaagtgat
     7801 gctggcgtac ttgctgattt tttattttgt tggcctgctt atttactata gcaagaaagt
     7861 ttcatgggat ctgttggatg tagtgttgat tggaggggca gttttattta ttggcggagt
     7921 catggttcat ttctggctaa cgtcaaaaga cgcattgtta gcttctttga ataccttata
     7981 tccaggaaaa cgggtaagta ctggaggaaa gtggacgatt ggcgaatttt tttctttcct
     8041 tacaaattgg aaaattcctt ttgaagatat caactattcg aataactctg aagtggccct
     8101 gttctatcac ttttttccaa ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa
     8161 agatagcgaa caaaagttac tgggacgtat tttgatgatt ttttgtctgt tttctatctt
     8221 ttggattacc gttgggttgc caaaatggct agcagaaatc actctgctgt cttatgttcc
     8281 tccattaaga gccatcctta cttttagttt tgcggcttgt ttattatcga tctggtttat
     8341 cgcttatatt tggagagaag atgttattcc tatttggtat aaattcattt tgttggcagt
     8401 aaatgcatgg tcttatttct atgcgattac gcattcgaaa atggataatt atttttcgga
     8461 ttttgaaacc atcgcagttg ctgtgttggg aacagtgatt ttagcttttg cattatttgg
     8521 ctggagacgc ttgttttacc tcgcttttgt cgccttagtt atcgtatctg gctttttcgt
     8581 caatccagtc gtagaaggta caggagcgat ttttgaaaaa acactggcaa aagaaatcca
     8641 aaaaatcgat caacaagatc ctggtaaagt ttggttgtct gaagatgaat tatataactt
     8701 caccccaaca ttgggagtga aatccttcaa tacagtacgc ttttatccag atatagctgc
     8761 atggaagaaa atcgatccga aagaagaata cgagaagtat tataatcggt atgctcatac
     8821 aagagctttt atcgctaagg aagatacaaa gttcatttta gatcgtccag atatgttttc
     8881 ggtcacgttg aattttgaag acgcagaaaa attagggatc gactatgtga tcagtaaacg
     8941 accattaact gattacaacc aattatatga agcgcagttc acgcagctat atggaccgga
     9001 taaagacggt tatatgattt ttaaagtaga atatccggat tatccagatg taccacaaac
     9061 acaaactcag gaaaatcagc tagtcgtaca gtaataggct agctgagaat aatcctgaaa
     9121 tattagtaga aaggagagtg aagataattc aagcaatcgt acaaaaattt gtcaacaaaa
     9181 tgagtgtcgg tttcttgctt ttgttgctga cgctagtctt ttttgtttgg acagcagggg
     9241 taggttttcc agcaactttg tttatatata acaatagttc attacttatc gcttttagtg
     9301 tggctatttt gctcatttta aatgtttata aagcaaaagg cgtcgatttt ctttatctag
     9361 gtgttgcgct tgcccttttc attttctttt cttactttcg ggaattaaga aatgagtctt
     9421 tatttggaga cttactgttt cctttggtta ttttattttg tttagtaatt aaatggatcg
     9481 agtggacaaa atgggatcgc ggtatttatc tgattgtctt tacggtcttc ctcggaatca
     9541 cgttatatcg tgtatttaca gagatccaag taccagaagg acaaagcatc tgggctccag
     9601 gcaattcgct ttctgatatt tggatcaata cgaatacaat cggaagttct ttgatgactc
     9661 ttgctttatt gatcagtgga tttgccagtt cttttgaacg atggtatgtt cgaatcatcg
     9721 gtgggatagc agtcgttgca gcctttttag gtatttgggt ctgccaatct agaggtgcat
     9781 tagtcgcgtt gattgttttt gcgatattgg atcttttacc taaatcgttg atgcgtgtca
     9841 ttcgtgcgcc atttattgcc tacacggtta cgattctttt agctttgccg atttcttatc
     9901 tggcggctgt ctctgaaaaa gtcaatttgt ttaccgatcg agaagatatt tggcataaat
     9961 tctatcagac gctaggagaa aaatctgaac aagttttact aggaatgaaa acatttatct
    10021 tccagcgagg aaaccaattt ttaggaaacc acaatagtta taattctatt ttgaatatct
    10081 atggattgat aggatttggg attgctgctt tgctgcttat cctttttatt ggtcgcttaa
    10141 cgttgaaagc agatttatcc aacgggcaga tgacttttat ctgggccttt tttgcggtga
    10201 tgatgcagtc atttatggag gatacattga cttcataccc atgggttccg attatttata
    10261 tcctgttggc tatggccgtt catcgatatg atgaaccaga agttgcggag gaattctatg
    10321 ttatggaaga ggcatatgac gatatgactt atgatgatac atcttatgag gaacaaccag
    10381 atagcgctca ttcttccagg atccaacgct atcattgata ggcacaaaat acgagagagg
    10441 ctgtgacaac cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa
    10501 tagaaacgtt agagaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct
    10561 cggtaccaaa aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg
    10621 aacacaaatg tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt
    10681 attgctacag ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat
    10741 cttatgggcg tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt
    10801 tggactgaaa gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg
    10861 gatgattccc tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga
    10921 atatacttat ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat
    10981 cctatccaat ctgtacaccc atttattctt cattctgatt ggcatagctg tcgcgcttat
    11041 aaatggtata tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg
    11101 tgctttcttg acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat
    11161 catgcaatta attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag
    11221 ccctaatcag tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt
    11281 tgttcaaggt tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata
    11341 tactttgtat ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg
    11401 tagattgaaa ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca
    11461 attagactaa aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt
    11521 cgtgaagccc tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac
    11581 gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga
    11641 aaatctacaa tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa
    11701 atcgaaggaa aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct
    11761 ggctatgaaa atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat
    11821 gaaatggtcg atgatatcat tgaatttgct gacatcggag accatattta tcaacctgta
    11881 aaaacttatt caagcgggat gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat
    11941 cccgatattt tgattgtgga tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa
    12001 tgtatggaaa aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat
    12061 gatatcagtt cgattcgtcg tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc
    12121 gtcgaatatg gcgatacgat ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa
    12181 tcagttaaaa cagtggatcg gaataaaaac caagtagaac ggttcgccgc tccagatatc
    12241 gtcgacgtga cgaaagcgga attgttggat aaagacaaaa atccggtgga tatgctggaa
    12301 caaggagaaa aactatacgt taaagtaact tatgaagtaa aagatgatac aatcaaaaat
    12361 ccggttttag gaattgcgct acgaacagtc gacaattttt atgtatgcgg acttaatact
    12421 ctactagatg gtatcaagat tccttggaaa aaaggagaga atatctttta tctggagtat
    12481 gaaaagttag gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat
    12541 gcaacggttc ctttggtata taagacaaag tacatgaact tgtttgtcag cggttcttat
    12601 atcggagaag gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa
    12661 atatgatttt gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat
    12721 caaaccagga agtaaagttt tagagtttgg tcccggaaac ggccgattaa cgaaacattt
    12781 aatcggagca aaacaatgtg aagtgagcat tgttgagtta gataaggaat tgttcgattt
    12841 tgtcagcgaa tttgctcaag atggatttta tggtgatatc gaaagttttg agtgggcaaa
    12901 ctattatgct ggacagacat ttgattacgt cttgtttgcg gatgttttag aacatttggt
    12961 agatcctgga aaaacattga agaaagtcag agaatttttg aatgaagaag gagagatcct
    13021 gattacattc cctaacttgg cgcataattc tgtcatgatc gatttgttca ataaccaatt
    13081 gccgtgggct tcttatggtc tcctagatga aacccataat tctttttata ctcatgatgg
    13141 cttccaaaaa gtttttgaaa aagcgggatt attcattaat attgaagatt atttgtactt
    13201 agctgtaggt gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta
    13261 tgatttcaaa atgcgaccat ttggtgaagt ctaccaatac tttttctcat tgatgaaaca
    13321 tccagtagca caaagttcca ttgcagagcc acaaaattct aattatgtaa aagtgttaga
    13381 agtaacccag caaacgaaac aagatgaaac aatccaacaa ataccattca ataatttcac
    13441 aggggaaaat gagacactaa gtttccccgt atcagaaaca acagaaagaa tggtgtttcg
    13501 cttcgcgcaa caacctagct ttatcgaatt cagcgcagaa gctgccggag aaaaactaac
    13561 atttatcgat tcgaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa
    13621 agaattacca gagttcgttc taacggatat ttctgggaaa gaagtgacta tccattgtca
    13681 ctatcgcttt atcggggaat tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc
    13741 catggctgaa gtcacaaagc agctttctga gaaaaatgac gaattagaaa gagaaaatca
    13801 tcatcttctt gaggaaaata caagactaga ccatacgtta aaaacaacca caaatcgcta
    13861 ttgcacattg ttggaaagtg atgaatggac aataaaacat agactaggac gacgtaagaa
    13921 agaaacttcg aaaaaaatcc aagaaaaaga actttctata tgtattgacg agaaaatctg
    13981 ggatgcagaa acaaaaatat taaaaatcat cggatgggga atcgctaatt cggatcgcca
    14041 gcccttgtcc tataagttat cagctgacca atcgccattt ttcgaagcaa ttcctgtttc
    14101 acgagaagaa gtcaatcaag cagaacagct cgcaaaagga acagaagcgg gatttgagtt
    14161 acgaatcctt tgcgagcaag aacgttcatt cttagtggaa gcagtagctc aaaatggcca
    14221 atcatggatc attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc
    14281 gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc
    14341 gatttgctta ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag
    14401 gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat
    14461 ttatataatc ttgatgttaa gacccaaagc ggtttcaagg tacgtttatc agggaaaatg
    14521 agaggaaaag ccatcttaga cttccaaaca gcgagacatc aaaatggcat agctgtgaaa
    14581 ttaaacggta aataccctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga
    14641 ttactgaaaa aagggataaa ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg
    14701 gtcaaattgg aattgaagcc aggatcgatc gactatgcag agtggatcag tcgtcatgag
    14761 aaaccagatt tgaaagagca aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt
    14821 tccatactta tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg
    14881 gtactggatc aaacgtatga tcattgggaa ctatgtatca gtgatgatgc ttctactgat
    14941 ccagctatcc gaaagtgtct ggaatcctat caagcaaaag atgatagaat caaagtcgtt
    15001 ttccgaaaag aaaatggaca catcagtctg gcaactaatt ctgcattgga aatagcagaa
    15061 ggagaattta tagcactctt agataatgat gatgaattac cgccatttgc attatatgaa
    15121 gtggcaaaag tgctaaacgt acatccagaa ctggatgtga tctacagtga cgaagataaa
    15181 atcgatgcag atggcaatcg ctttgatccg catttcaaag cagactggtc gcctgataca
    15241 ttgatgggaa ataattatat ctctcactta ggcgtatatc gaacaagcat tgttaaagaa
    15301 cttggcggtt tccgtaaagg ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg
    15361 acagaacaaa tccctgcaga tcatatttac cacattgacc gagtcttgta tcattggcgg
    15421 acgatcccag gatcaactgc aagtaatggc gaagccaaaa gttatatcta tgattccgga
    15481 gtgaaagcat tgacagatgc cctgtctaga agaaacatca aaggaagcgt acgtcctggc
    15541 agaatttctg gattttatga gattgcttat gacgtcttgc aagaggattt ggtcagtgtg
    15601 atcattccaa cgaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa
    15661 aaaacgtctt attcaaatta tgaaatcatt attgcggata acggaagtac agatccaaaa
    15721 atgcaagaat tatttgcaga atacaagcat cagctgaaag accgctttat cgtagaattg
    15781 attgacattc cattcaatta ttctcgaatt aacaatttag cagcagaaaa agcaaacggc
    15841 aaatactttt tattcttgaa taacgataca gaagtgattg agccagattg gatgacggtg
    15901 atggtttctt atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact gttttatcca
    15961 gatgatacaa cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc agggcatgca
    16021 ttgaataact atgatcgaac acattgtggt tatttcggcc gattggtcat tgacgtcaat
    16081 tacttagcag tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac
    16141 gggtttgatg aaacattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat
    16201 gaactgggtc gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa
    16261 tcaagaggtt atgaagatac tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa
    16321 atgcaagata aatggccagc atatattgcg catgatccat tttacaatga taatttaaca
    16381 aaagacggaa tcggcgactt ttcgttgaga ccagattaaa ctataataaa aagctgttat
    16441 gttgtgattc gctcgcacat aacggctttt tatagttttt tgtcaaaaaa aggcagaagg
    16501 tgccgactca aaaaaataag tcgaggttgg gacagaagtg tttaactccg agaaataaga
    16561 agaaattcac gaaaattgct tttcaaattt ttgtgaattt cggcttattt aagaagaagt
    16621 tgcttctgct cccaccgttt attcgttttt aaagaatgag acaaaagtgt ttcttacttt
    16681 tgtcccacgc tcttattcat gctcagagcg ccaatagata caaaatcata gccaaagttt
    16741 cacgaaccct agctgtcaaa gaatcgatat tttattttaa tataggctat tttaaaataa
    16801 atggtttaag gtattcctct acaaaagcca ctctatcaat tatcatgggt ttaaaataag
    16861 aagtgatgga gataaccaga ttgttcttag cgtcaatata aagaacattt ccactattac
    16921 caattgctga atatatttcg ttctcagaat ctaataccca ccataaatag ccatatctca
    16981 tattttgtaa gtcttctaaa gcactggttc taggtttgaa catctcgttt atccacatgg
    17041 gtggtacgat ttgttttcct ttatacatcc ctttatttag gcataataca ccaatttttg
    17101 ccagatcact tgccgataaa cacaaaccat atcctgccgt cccgatattt agtggatcac
    17161 aaaaccagac attggtttta ggagacttat ccattgtaaa ctgcttatgt tcttcagctg
    17221 tttcagcata ataattgaca tggtttggta tccctatagg atcaaataga tacttattgg
    17281 caaaatcaac agttgttagc ttacttgtct gatacaacaa gccagttaat atgtgtaaac
    17341 agacagtttg atagttgaat tcgccggtta taccttttct tcctcccaat aaatctagtg
    17401 atgtttttgt ccaatcttca cttgaacaga cttttgtcca tggatcgcct ttacatttgt
    17461 agggtgcttt cattgttagc aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc
    17521 gtttcacttt atactctgga aaataatcta atactttatc atcgatactt ttgattaatc
    17581 cttgctctaa agcaatgcca atcagcaaag atacaatact tttcgttgct gacatgatat
    17641 gacaagtatc gtctttttta taaccattcc attcatctgt ataaacttct accccatcct
    17701 tatatgcgac aatttgacat atatttggtt gtcgaaaacc aataaaatca tgcaactctt
    17761 gtttattcat aagatattac ctcactttct tctataagca taatgtcatt aaaaatcgat
    17821 ttcaattttt tcgattcttc acgtaataca ggtattctta tggactagac agcgctttgg
    17881 gaagatggat tctctgaaaa atataaaggt tcactttatg agtggataaa aaaagcagca
    17941 aatacttcga cttctttaat ttaccaacat tagtgaaaat cgattcatct ggagatttgt
    18001 aatttagagt ccttgtcttg attagaggtg tataaagctt tttattagac atttttaaat
    18061 tccattacaa tttgcttata aactaggttt ttaaaggtga ctatggtaag ataatagaga
    18121 tattattttg tgattaggag tgtatatatg aataaaaatg gagaatggag tgctgcacga
    18181 cggatattca ttatattaat ggatgttatt gtatacaatg cagcaatata tagtagtttc
    18241 ttactgaagt ttcgtggaga gatcccatta cgaaatttag aaactttcca acactcagca
    18301 attattattt cagtgatttt tattgcgttg aacattttgt tgggtgcata tgttttttat
    18361 aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta
    18421 gggatcatgg tagtgacttt tgcgggccgc tggtttgctt ttccacgtct tgttatctta
    18481 ctttcatttg ttctcggtac gcttttctta agtatctatc gtattctcgt atataaactt
    18541 tatttacgcg taagtcgaga taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca
    18601 ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt
    18661 cttgataact attatgcaaa catcaaaaat atgattaaag acatcgatat tgtctatctt
    18721 gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc aattagttac taaaaatgaa
    18781 aaaaaattgt ttttgaatac aacatttgaa aacttgacat tgatcaatcc aaatattatg
    18841 aatttcgaag atgaaagtat tattgaagca tctggattta gaatccaacc tgaatatgaa
    18901 ctattcaaac gtttgtttga tatcatcgtt tcacttgttt tattagttat tgcttcacca
    18961 tttatgttat tcacagcaat tttagtcaaa gtgacatcac cgggaccaat catctataaa
    19021 caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc
    19081 gccacagccg aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc acgagtaacg
    19141 cctgtaggta aatttattcg agctgtccgc tttgatgaat taccacaaat cttcaatgtg
    19201 ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac gttctttctt cgtcgatcag
    19261 ttcaatgcag aaaaccctta ttactactta cggcataatg ttcgtgctgg tatcactggg
    19321 tatgcacaag tctatggaaa atatgtctct gattacaaca gtaaattacg ttttgattta
    19381 ctttatatca aaaaatattc attgatgctt gatttaaaga tcttgctgca aaccatcaag
    19441 atcctatttg ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt gatctcattg
    19501 gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac
    19561 aatccgaaat tcagcataat cgtaccagta tacaatgcag aagcaactat tggtcgaacg
    19621 attgatattc taaaagaatt ggattatgaa gagtacgaag tcgttttagt aaatgatgga
    19681 tcaacagatc aaacacagga aatcattcaa gaactgattg aaaatgatga acgttttgaa
    19741 ttgattacta cgaccaatca agggcccgga ttagcaagaa acgaaggaat aagacagtca
    19801 aaaggagaat atctcttatt cttcgatgca gatgacacgc cagtaaaaac gatcttgtca
    19861 gattatgatc atttgctaga aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt
    19921 tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt
    19981 cactatctaa ctcacgaaga gtttataaaa gacatgtatc agttaatgaa tgaacagttg
    20041 atgtatgttg tatggaacaa atgctatcgt agcgatattc tgaaaaataa ccaaattcta
    20101 tttaaaactt ataatagttg tgaggatcga atatttaact tacattatta tgaatactgt
    20161 catcaagtga tcatgaaccc aaaagtagaa tatatttatg aatttgaagg tgggaagggg
    20221 atcactaatc aataccgacc taacaagttc gaaacattta aagaattcta ttcattagca
    20281 aatactgtaa cagacgatat taataaagac ggtatggcgg cgcttctttt aaaagggaca
    20341 acttcagtta ttttttctat ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac
    20401 gaagcaaagc agatattgag tgatccagtg atccgagaag ccaagcaaat tgctaagacg
    20461 gattcttcag caaaaaaaat aacaaagttg ctttacaata tgccagctcc actattttta
    20521 tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg
    20581 aagcgagtat attagttaat ggaggaaaat ataatgaaaa gaattttgca ttttcaggga
    20641 cgaatgggtt taggtggagc agagtctttc atgatgaatt tatatcgtaa aattgatcgt
    20701 acaaactatc aatttgattt tttgatttat gaagattatg cagatgttca agactatcat
    20761 tcagagattg aaagattagg aggccgtatt tttgttgtac ctaatcctaa gaaaaatatt
    20821 tttaaatatt taatagaagt gaataagtta ctaaaaaaag aatctttttc tatagttcac
    20881 aatcaggttt attttggcgg gggaattaat ttatggttag ccaaaaagaa tggaatcaga
    20941 cagagaattg ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat tgtaatgaat
    21001 gtattgcgta aatttttaac caaattgttg ttacaaaatg caacagacta ccttgccgtt
    21061 agtcaagaag ctggtgaaag tctgtttcaa aaccatccct ttgagatagt tcataatggt
    21121 attgatttag agctttatag taaaaattca gaagctaagg ttaataagag aaaagaattg
    21181 gatatctcaa tgagtacttt tgttgtggga aacattggac gattagagaa acaaaaaaat
    21241 caatcttatt tgttagagat ttttttagaa atactaaaaa tgaattctga ttcttacttg
    21301 attttagtag gtgaagggtc acttagaaat aaactgaaag ccaaagcagt agaattggat
    21361 atagagaaaa gagtaaaatt tttaggagaa agaaacgata ttcccgagtt attatctgtg
    21421 atggatgttt ttgtaatgcc ttctctttat gaaggtctgc caatggttgg attagaagca
    21481 caagcagcaa aattaaaatt agtcttagca gatactattt ccaaagatac aaagttaaca
    21541 aataatgtgg attttgtatc attagatgaa acaccacaag aatgggcaaa aaaagtaata
    21601 aaagagccgt atgctaataa aataacacaa gaactaaaga gttatgatgt ttcgtacact
    21661 actgaacaaa tggagagtat atatagtaga gatcattaaa aatgttcaag atattttagg
    21721 agtgattatt tttgaaaata tgttatttga tattggcata caatgatatt ggcaacttaa
    21781 agagattggt caatcgattg aacgaaaatg cagtttttat tattcatata gataaaaaaa
    21841 ctgatataga accatttatt gaagcattta aatcatatga taatgttgaa tttttatgta
    21901 ataaaaaccg tgttaaaatt tattggggtg gtttttcca
//
LOCUS       DANJJH010000044        21375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000044 DANJJH010000000
VERSION     DANJJH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            56..757
                     /locus_tag="PWS53_001541"
     CDS             56..757
                     /locus_tag="PWS53_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0871771.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="PWS53_001542"
     CDS             complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="PWS53_001542"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL0871772.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="PWS53_001543"
     CDS             complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="PWS53_001543"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0871773.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="PWS53_001544"
     CDS             complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="PWS53_001544"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0871774.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="PWS53_001545"
     CDS             complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="PWS53_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL0871775.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4289..5269)
                     /locus_tag="PWS53_001546"
     CDS             complement(4289..5269)
                     /locus_tag="PWS53_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871776.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5293..6777)
                     /gene="galT"
                     /locus_tag="PWS53_001547"
     CDS             complement(5293..6777)
                     /gene="galT"
                     /locus_tag="PWS53_001547"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL0871777.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6789..7778)
                     /gene="galE"
                     /locus_tag="PWS53_001548"
     CDS             complement(6789..7778)
                     /gene="galE"
                     /locus_tag="PWS53_001548"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL0871778.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8026..8193
                     /locus_tag="PWS53_001549"
     CDS             8026..8193
                     /locus_tag="PWS53_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL0871779.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8255..10066)
                     /locus_tag="PWS53_001550"
     CDS             complement(8255..10066)
                     /locus_tag="PWS53_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL0871780.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10063..10428)
                     /locus_tag="PWS53_001551"
     CDS             complement(10063..10428)
                     /locus_tag="PWS53_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL0871781.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10591..10986)
                     /locus_tag="PWS53_001552"
     CDS             complement(10591..10986)
                     /locus_tag="PWS53_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0871782.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11004..11966)
                     /locus_tag="PWS53_001553"
     CDS             complement(11004..11966)
                     /locus_tag="PWS53_001553"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL0871783.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11966..12178)
                     /locus_tag="PWS53_001554"
     CDS             complement(11966..12178)
                     /locus_tag="PWS53_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL0871784.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12199..12897)
                     /locus_tag="PWS53_001555"
     CDS             complement(12199..12897)
                     /locus_tag="PWS53_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL0871785.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12917..13459)
                     /locus_tag="PWS53_001556"
     CDS             complement(12917..13459)
                     /locus_tag="PWS53_001556"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL0871786.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13591..14595)
                     /locus_tag="PWS53_001557"
     CDS             complement(13591..14595)
                     /locus_tag="PWS53_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0871787.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14592..15581)
                     /locus_tag="PWS53_001558"
     CDS             complement(14592..15581)
                     /locus_tag="PWS53_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0871788.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15578..16384)
                     /locus_tag="PWS53_001559"
     CDS             complement(15578..16384)
                     /locus_tag="PWS53_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871789.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16550..17506
                     /locus_tag="PWS53_001560"
     CDS             16550..17506
                     /locus_tag="PWS53_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0871790.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17583..18101)
                     /locus_tag="PWS53_001561"
     CDS             complement(17583..18101)
                     /locus_tag="PWS53_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0871791.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18189..18338)
                     /gene="rpmG"
                     /locus_tag="PWS53_001562"
     CDS             complement(18189..18338)
                     /gene="rpmG"
                     /locus_tag="PWS53_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0871792.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18566..19012
                     /locus_tag="PWS53_001563"
     CDS             18566..19012
                     /locus_tag="PWS53_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL0871793.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19207..21102)
                     /gene="asnB"
                     /locus_tag="PWS53_001564"
     CDS             complement(19207..21102)
                     /gene="asnB"
                     /locus_tag="PWS53_001564"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL0871794.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6425 a         4545 c         3692 g         6713 t
ORIGIN      
        1 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
       61 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      121 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      181 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      241 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      301 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      361 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      421 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      481 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      541 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      601 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      661 agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt
      721 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      781 cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
      841 gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
      901 cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc
      961 tagattattc gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg
     1021 gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa
     1081 aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg
     1141 tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg
     1201 ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa
     1261 agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg
     1321 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac
     1381 caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg
     1441 tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag
     1501 ttctacctca gggagtttag ctgccaaagc atcctgcaat ccttcacgca acccttgatt
     1561 ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag
     1621 tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt
     1681 gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa
     1741 tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt
     1801 atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa
     1861 tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat
     1921 ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag
     1981 tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat
     2041 atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat
     2101 accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc
     2161 ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac
     2221 gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg
     2281 agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc
     2341 atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt
     2401 aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta
     2461 cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt
     2521 acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa
     2581 tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact
     2641 tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc
     2701 agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa
     2761 ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca
     2821 aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc
     2881 aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc
     2941 aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc
     3001 tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt
     3061 ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct
     3121 cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga
     3181 agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag
     3241 acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg
     3301 taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag
     3361 cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc
     3421 caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc
     3481 tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca
     3541 tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt
     3601 gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa
     3661 atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg
     3721 gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc
     3781 cacaccgatg caattagccg cgatggtttt cagactagat actcctacta gctctttatc
     3841 taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc
     3901 gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa
     3961 caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat
     4021 ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc
     4081 tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt
     4141 agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat
     4201 gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca
     4261 ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg
     4321 tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct
     4381 ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta
     4441 catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta
     4501 aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa
     4561 gcgcatcagg aacatgcgga ttcgttttta aatattgctt cactgcttga tatcctgact
     4621 cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt
     4681 gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg
     4741 ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt
     4801 gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat
     4861 ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt
     4921 ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca
     4981 ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat
     5041 aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag
     5101 tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt
     5161 tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag
     5221 tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct
     5281 ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc
     5341 tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg
     5401 tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc
     5461 ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc
     5521 tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc
     5581 aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg
     5641 ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct
     5701 tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga
     5761 tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag
     5821 caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc
     5881 gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg
     5941 aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg
     6001 taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct
     6061 agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg
     6121 ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat
     6181 tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt
     6241 ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat
     6301 ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt caccatactc
     6361 cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt
     6421 cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc
     6481 aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa
     6541 aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag
     6601 ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc
     6661 atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca
     6721 tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc
     6781 tcattcccct attcttcata accatttgga tggctgacat gccaatccca tgctgttttg
     6841 ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca
     6901 cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt
     6961 tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg
     7021 ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata
     7081 tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta
     7141 tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg
     7201 attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct
     7261 ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac
     7321 ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg attagctggt
     7381 gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat
     7441 acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg
     7501 ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca
     7561 aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc
     7621 cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc
     7681 aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa
     7741 ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt
     7801 aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa
     7861 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
     7921 attttcaagg gttttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
     7981 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
     8041 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggttgcttct
     8101 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
     8161 aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat ttaaaaatac gcatgaagac
     8221 tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
     8281 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
     8341 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
     8401 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
     8461 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
     8521 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
     8581 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
     8641 caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
     8701 tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
     8761 gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc cggattggat
     8821 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
     8881 ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag ggctcattct
     8941 gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt agtgctggat
     9001 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
     9061 gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
     9121 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
     9181 tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga tttcttcctc
     9241 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
     9301 gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat actgtaaaca
     9361 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
     9421 gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt aaaacatttt
     9481 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
     9541 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
     9601 cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
     9661 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
     9721 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
     9781 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
     9841 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
     9901 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
     9961 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    10021 ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    10081 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    10141 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    10201 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    10261 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    10321 taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga gtattctata
    10381 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    10441 ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    10501 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    10561 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    10621 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    10681 atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag gctggtcttt
    10741 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    10801 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    10861 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata
    10921 gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    10981 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    11041 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    11101 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    11161 actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt tccgcaagct
    11221 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    11281 gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat tgctttcagt
    11341 tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac acctgtagcg
    11401 ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    11461 acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa atttccgcca
    11521 gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    11581 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg
    11641 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    11701 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    11761 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg attgattgat
    11821 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    11881 ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    11941 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    12001 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    12061 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    12121 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    12181 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    12241 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    12301 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    12361 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    12421 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    12481 taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac cagacagaac
    12541 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    12601 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    12661 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    12721 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    12781 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    12841 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    12901 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    12961 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    13021 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    13081 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    13141 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    13201 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    13261 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    13321 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    13381 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    13441 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    13501 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    13561 aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat
    13621 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    13681 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    13741 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    13801 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    13861 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    13921 ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa gtcctcctag
    13981 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    14041 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    14101 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    14161 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    14221 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    14281 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    14341 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    14401 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    14461 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    14521 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    14581 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    14641 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    14701 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    14761 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    14821 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    14881 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    14941 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    15001 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    15061 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    15121 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    15181 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    15241 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    15301 cctagtaagc ctgaatctgc taaaggattt cttgtcaccc cttgcatcaa tgcaccgctc
    15361 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    15421 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    15481 ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    15541 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    15601 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    15661 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    15721 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    15781 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    15841 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    15901 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    15961 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    16021 actcaattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    16081 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    16141 ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    16201 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    16261 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    16321 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    16381 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    16441 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    16501 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    16561 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    16621 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    16681 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    16741 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    16801 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    16861 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    16921 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    16981 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    17041 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    17101 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    17161 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    17221 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    17281 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    17341 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc taaaagcagt
    17401 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    17461 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    17521 agcatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    17581 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    17641 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    17701 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    17761 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    17821 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    17881 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    17941 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    18001 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    18061 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    18121 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    18181 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    18241 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    18301 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    18361 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    18421 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    18481 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    18541 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    18601 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    18661 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    18721 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    18781 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    18841 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    18901 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    18961 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    19021 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    19081 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    19141 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    19201 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    19261 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    19321 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    19381 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    19441 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    19501 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    19561 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    19621 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    19681 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    19741 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    19801 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    19861 tgattcatat aaatggatcc gtccatggaa gtttttcatt tttccgattt tacgtcctaa
    19921 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    19981 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    20041 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    20101 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    20161 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    20221 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    20281 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    20341 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    20401 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    20461 gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    20521 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    20581 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    20641 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    20701 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    20761 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    20821 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    20881 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    20941 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    21001 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    21061 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    21121 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    21181 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    21241 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    21301 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg
    21361 acctaaagat cgtgt
//
LOCUS       DANJJH010000045        21233 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000045 DANJJH010000000
VERSION     DANJJH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(37..684)
                     /locus_tag="PWS53_001565"
     CDS             complement(37..684)
                     /locus_tag="PWS53_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871795.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(863..1582)
                     /locus_tag="PWS53_001566"
     CDS             complement(863..1582)
                     /locus_tag="PWS53_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871796.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1713..2864)
                     /locus_tag="PWS53_001567"
     CDS             complement(1713..2864)
                     /locus_tag="PWS53_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL0871797.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3081..3821)
                     /locus_tag="PWS53_001568"
     CDS             complement(3081..3821)
                     /locus_tag="PWS53_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0871798.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3825..4148)
                     /gene="rsfS"
                     /locus_tag="PWS53_001569"
     CDS             complement(3825..4148)
                     /gene="rsfS"
                     /locus_tag="PWS53_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL0871799.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4205..4798)
                     /gene="yqeK"
                     /locus_tag="PWS53_001570"
     CDS             complement(4205..4798)
                     /gene="yqeK"
                     /locus_tag="PWS53_001570"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL0871800.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4782..5432)
                     /locus_tag="PWS53_001571"
     CDS             complement(4782..5432)
                     /locus_tag="PWS53_001571"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL0871801.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5442..5753)
                     /gene="yhbY"
                     /locus_tag="PWS53_001572"
     CDS             complement(5442..5753)
                     /gene="yhbY"
                     /locus_tag="PWS53_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL0871802.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5769..6878)
                     /gene="yqeH"
                     /locus_tag="PWS53_001573"
     CDS             complement(5769..6878)
                     /gene="yqeH"
                     /locus_tag="PWS53_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL0871803.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6875..7402)
                     /locus_tag="PWS53_001574"
     CDS             complement(6875..7402)
                     /locus_tag="PWS53_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL0871804.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7582..8367)
                     /locus_tag="PWS53_001575"
     CDS             complement(7582..8367)
                     /locus_tag="PWS53_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL0871805.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8360..9229)
                     /locus_tag="PWS53_001576"
     CDS             complement(8360..9229)
                     /locus_tag="PWS53_001576"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL0871806.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9230..10609)
                     /locus_tag="PWS53_001577"
     CDS             complement(9230..10609)
                     /locus_tag="PWS53_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL0871807.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10617..11036)
                     /gene="fabZ"
                     /locus_tag="PWS53_001578"
     CDS             complement(10617..11036)
                     /gene="fabZ"
                     /locus_tag="PWS53_001578"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0871808.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11061..11537)
                     /gene="accB"
                     /locus_tag="PWS53_001579"
     CDS             complement(11061..11537)
                     /gene="accB"
                     /locus_tag="PWS53_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL0871809.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11540..12775)
                     /gene="fabF"
                     /locus_tag="PWS53_001580"
     CDS             complement(11540..12775)
                     /gene="fabF"
                     /locus_tag="PWS53_001580"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL0871810.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12794..13531)
                     /gene="fabG"
                     /locus_tag="PWS53_001581"
     CDS             complement(12794..13531)
                     /gene="fabG"
                     /locus_tag="PWS53_001581"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL0871811.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13543..14460)
                     /gene="fabD"
                     /locus_tag="PWS53_001582"
     CDS             complement(13543..14460)
                     /gene="fabD"
                     /locus_tag="PWS53_001582"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0871812.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14567..14791)
                     /locus_tag="PWS53_001583"
     CDS             complement(14567..14791)
                     /locus_tag="PWS53_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0871813.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14844..15806)
                     /locus_tag="PWS53_001584"
     CDS             complement(14844..15806)
                     /locus_tag="PWS53_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL0871814.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15816..16256)
                     /locus_tag="PWS53_001585"
     CDS             complement(15816..16256)
                     /locus_tag="PWS53_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0871815.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16562..17527
                     /locus_tag="PWS53_001586"
     CDS             16562..17527
                     /locus_tag="PWS53_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0871816.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17584..17814)
                     /locus_tag="PWS53_001587"
     CDS             complement(17584..17814)
                     /locus_tag="PWS53_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL0871817.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17900..18787)
                     /locus_tag="PWS53_001588"
     CDS             complement(17900..18787)
                     /locus_tag="PWS53_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0871818.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18876..20099)
                     /locus_tag="PWS53_001589"
     CDS             complement(18876..20099)
                     /locus_tag="PWS53_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0871819.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20116..21162)
                     /locus_tag="PWS53_001590"
     CDS             complement(20116..21162)
                     /locus_tag="PWS53_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0871820.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         5753 a         4707 c         3594 g         7179 t
ORIGIN      
        1 aattgcagaa aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc
       61 tgtttcagga tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc
      121 tggatattct gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg
      181 gtaaattcga ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc
      241 gccacaatcc aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt
      301 ttttttcgtg tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa
      361 gctccactct aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa
      421 ggctgaatca gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat
      481 tggtgcgcgg tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt
      541 tcctaatcga tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc
      601 agttacgttc acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg
      661 ttctaaagtg atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca
      721 tttcacatga tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc
      781 acacaaaaaa gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat
      841 acttttcgtt aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta
      901 catcttctag atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag
      961 atagtgttac ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc
     1021 ctttttcttt taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga
     1081 tttgtccttc ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa
     1141 ggatctcgtc agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag
     1201 aaaccgctcc ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg
     1261 ctgctgttcg attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc
     1321 caaatccttc atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga
     1381 ttgctcggtc aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac
     1441 gtagtttttg gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa
     1501 tcccaaattt tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga
     1561 tatttgccca tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg
     1621 caaaaacgtc ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc
     1681 ttccttcatt cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa
     1741 ttctgttccg gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc
     1801 gcttcttgtg cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt
     1861 aagaatcttc ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg
     1921 acaggaatct cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt
     1981 gtccatgtat atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt
     2041 gcagctgctg ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt
     2101 tccagcaaag tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc
     2161 tgcagcgaac aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt
     2221 tttccttctt ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg
     2281 attttttcat gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca
     2341 tacttggcat tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca
     2401 agcgtaatat cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac
     2461 gtttgatcag acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag
     2521 cgtccgaaac tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca
     2581 cagtccagtg tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc
     2641 gcccattcga cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg
     2701 cgatgccatt tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca
     2761 gcaatgacga cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca
     2821 ttgtgaaaag gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat
     2881 agttatcgat aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc
     2941 cgaactatat aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa
     3001 gagcaatgcc tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt
     3061 aactcgagca ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc
     3121 tctgatgctg cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt
     3181 tccagcattc ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca
     3241 cgaataaatc gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg
     3301 ctgtgtttct cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa
     3361 tgatagctat attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa
     3421 aatcggccat tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt
     3481 ctatttttca tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt
     3541 tctgataaat cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct
     3601 cgctggctgg caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca
     3661 tcttttgcaa aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt
     3721 gtgtcttctt gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc
     3781 atcacttcat cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca
     3841 ctaagatcga ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct
     3901 ggcgcatgga aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct
     3961 tcttttcctt caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct
     4021 tctgtaattg cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc
     4081 aacaaggaaa catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct
     4141 gcttcaatcg caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag
     4201 cagcttattt ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt
     4261 gttcttgcgc aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta
     4321 gatcagccca tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa
     4381 tatagtctgc tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat
     4441 gcacccggat tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg
     4501 aagcgccaac aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac
     4561 cgtctcttac gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg
     4621 tcaatgcagc aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg
     4681 ctgtttcttc tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt
     4741 tttgcatcag gttttctcta gaataagcag tatattcatt actatagtcc atattcatac
     4801 aacccttcat tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag
     4861 ccttctttga tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc
     4921 caaattacag gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg
     4981 accatagaag tcagctcatc gattttatac cattttggca aatattctac catatcccca
     5041 ccgataataa agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca
     5101 taggtgtaac ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg
     5161 tcttccaccg ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca
     5221 tcaacatgag gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg
     5281 ccagcttgtt cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag
     5341 atccctactt gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa
     5401 cgaacaaatg tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct
     5461 tttgatagcc tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca
     5521 atgatttgga cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca
     5581 atctcatctg tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct
     5641 tcaatttgaa cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga
     5701 agatgatgcg ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc
     5761 cttcttcctt aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc
     5821 gcaattacta ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt
     5881 ttttctttca cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga
     5941 cgaggaggct gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt
     6001 ttcgttcgat gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg
     6061 ctgataaaat caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga
     6121 taaactttag gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag
     6181 tagtgcgcca tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca
     6241 tctaaaggaa tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca
     6301 tcttgtactc ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt
     6361 gttactccga cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt
     6421 tccgtcatct catgagattt cttagcacta gttaataata catctactgg acgaattcct
     6481 gcttcgtaag cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt
     6541 aatatatcca ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt
     6601 cctgggatta gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct
     6661 ttttgcccta attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg
     6721 gtctcattat aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa
     6781 cctttttcca aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata
     6841 acggcaccac agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca
     6901 tatcagggtg ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt
     6961 gtgtcttcca actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac
     7021 gattggctcc acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg
     7081 ctaaatccaa ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg
     7141 caacatacaa aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg
     7201 aaacaacaac aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt
     7261 ctgttccatc tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt
     7321 tgatgcctag attctttaat tgggcaggtg taatctgata gatggcttct accatccatg
     7381 ttggtttgta atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa
     7441 agtactcctc aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt
     7501 tcgtctttca gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa
     7561 aaatttagaa acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa
     7621 tttatgctga tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat
     7681 gtgtgcgact ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc
     7741 aacgattcct aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc
     7801 ggcgcgacta ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata
     7861 aatcgtttct tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact
     7921 tccaccttca ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag
     7981 atttctggca atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc
     8041 tgctgtattg atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg
     8101 taaagctttt cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg
     8161 cagatcccgt cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat
     8221 tccaccaaca atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga
     8281 atcgaagatc tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg
     8341 aacgacatca tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa
     8401 gaagcgtatg gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat
     8461 gagataataa gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt
     8521 cgatgacgcg tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc
     8581 cttccattgc aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat
     8641 aaaaaagacc ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta
     8701 gagaaaagat tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa
     8761 gactttcctt tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca
     8821 tacttcccat gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg
     8881 ttgctttacc agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt
     8941 tttccgtata ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt
     9001 caaaactttt ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt
     9061 atccgcaatg agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc
     9121 aatttggaca ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct
     9181 ctctattagg attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc
     9241 ctatctccgt ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt
     9301 aatcacctgc tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga
     9361 tgattccttc tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac
     9421 gcgtatttcc atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat
     9481 atcccgggta aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt
     9541 tttggatctt ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc
     9601 attcgattgc gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg
     9661 ctgctatctc cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat
     9721 gttccacttg gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca
     9781 gaaactcgat cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt
     9841 ctcctagtgc ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt
     9901 tttgattgtt gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc
     9961 gatcacctaa gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca
    10021 tatcatcatt tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga
    10081 gctcttcttt tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca
    10141 gcatcactgg ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata
    10201 caccagtact tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga
    10261 tcttatttcc cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact
    10321 cttcacacat ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg
    10381 cttcggcttt tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa
    10441 cggcttttgc cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg
    10501 catctgcttc tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga
    10561 tacgtacagc gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat
    10621 ccaatcataa aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct
    10681 ttgcctattc cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct
    10741 cccggtgtaa ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt
    10801 cccttgaatt gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca
    10861 atcaataccc ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg
    10921 atcgttacat ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca
    10981 atcaaaagca ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt
    11041 ttccctcttt cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg
    11101 acaactgact cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc
    11161 tcattcatca gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca
    11221 cctacttttt tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt
    11281 ggcgaaacaa cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga
    11341 gcagttgttg cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt
    11401 gtttgaacca tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga
    11461 ccttcacgca aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg
    11521 atttcattga tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc
    11581 cgccaaaccc taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt
    11641 tgacaactgt caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa
    11701 attgatgttc taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata
    11761 aatgtccagt catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg
    11821 cgatttggat tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat
    11881 tgatatatcc gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt
    11941 tagatgcacc agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac
    12001 cgtatccaac gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt
    12061 caagaactag aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg
    12121 gaatcgaacc acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag
    12181 caatccccat ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc
    12241 catgtttgat gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta
    12301 cgatagctgt acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt
    12361 tggcaatcgc cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac
    12421 gaataacttg gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa
    12481 accggtcttg attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag
    12541 cgtagattcc atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt
    12601 tatcgtaagg gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa
    12661 atttagtgat cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg
    12721 cgtcatttcc aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt
    12781 tgctctcctt ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg
    12841 tgatatatgg attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga
    12901 aacgctgcat cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg
    12961 tcatatcagt tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag
    13021 cttctcgtgc cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg
    13081 cttgaccaat atttccaatc aacccagata cactagagag attaatgatg gccccttctc
    13141 gctgcttcat catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga
    13201 tatccagaca ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga
    13261 ttccggcatt gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca
    13321 tttgtcccgc tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga
    13381 atgcttcgat ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat
    13441 ttgctccagc ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg
    13501 tgataaatac attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt
    13561 ttcatcaaat gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt
    13621 cataaatccg cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt
    13681 ttttaactta tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag
    13741 tcccgcgatt gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga
    13801 aggttcagag aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa
    13861 aatagctgta tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc
    13921 ttttaatagt aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg
    13981 aggtgtatta taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc
    14041 ctcgatcgtt tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc
    14101 agcttctgtc atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag
    14161 cgcattacta gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg
    14221 gatgattcca tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg
    14281 ctgtgtatat tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc
    14341 gtagccgaga atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa
    14401 atcttttccc ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat
    14461 gtttttctcc aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac
    14521 attcctattt caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt
    14581 tttctacgta agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca
    14641 cgtcaaattc atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta
    14701 agtcttcttg gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg
    14761 cgatgatttc ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct
    14821 ttttattttt ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct
    14881 ccgccatagc ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa
    14941 actgcttcat ccaataagat agggatgctt gcagcagatg tattcccata tttatccata
    15001 ttggtcagga acttttcttg cggcatcttt acttttcggg caattttctc aataattctg
    15061 aggtttgcct gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata
    15121 ttgtttgcaa cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa
    15181 tacgaacctt ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc
    15241 aatgagctac ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga
    15301 ttcgcttcta agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca
    15361 gaccaatcga ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg
    15421 cagcggatca ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg
    15481 atatcaaaag cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat
    15541 ggtgtcgtgt aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt
    15601 agatttttct gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga
    15661 tttgttacac atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta
    15721 tccatcatct tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt
    15781 gcagtaatca ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg
    15841 tgcaagtttt tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag
    15901 atgttcttga ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt
    15961 ttcgttagtc ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag
    16021 ccttttttta ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct
    16081 ttggctactt ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc
    16141 tctgtgattg acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa
    16201 atatcattga agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc
    16261 caccgtccac taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt
    16321 ttttcaaaat gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt
    16381 actcatttaa aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct
    16441 atctcacata ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa
    16501 acttgtccat tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc
    16561 tatgttaaaa gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga
    16621 ccgagatggt aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag
    16681 ctattttcat cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac
    16741 agattttgat ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt
    16801 gatccaacat taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta
    16861 tgtacatatt accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg
    16921 gccttttcgt ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca
    16981 aatgccttgg ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca
    17041 ggaaatgact tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa
    17101 aaagctactg acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga
    17161 aaaagccggt atgcttcaaa aagatatcga attagttact tattttatta gacggatcca
    17221 ggaacaaaag cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga
    17281 tgagagtcag aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac
    17341 ctctggtgac gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa
    17401 acgtcaattg tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa
    17461 attagaaaaa gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact
    17521 aaattagttg cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa
    17581 aatttattgg attaagtcat agatttccat cgctacgata tcgatattat caaattcgta
    17641 ttgctgatga gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac
    17701 tgtacacttc tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga
    17761 ctcgaccata gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg
    17821 tcctcctttt cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt
    17881 ctctaaaaaa actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa
    17941 gtttgatcaa agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata
    18001 gtttcaatgc ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg
    18061 caatctttaa atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta
    18121 aaatacgagg agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct
    18181 caatcacttt ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc
    18241 ctgtcatcgt acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag
    18301 cagaaccatg caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat
    18361 aaacggcttc ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac
    18421 caccggatac cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt
    18481 tttctgccag gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg
    18541 cagaaaaaat ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca
    18601 ttgtaaagag aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc
    18661 cttctgttac taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc
    18721 ctgcttctag tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa
    18781 gtttcatttt ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta
    18841 gaaacaagga ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt
    18901 attttgattc gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa
    18961 acaaccatgt cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca
    19021 taaagtttag cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata
    19081 agtgctggtg caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac
    19141 aatcggttca aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct
    19201 gtataagtaa gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta
    19261 taataaagtg ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac
    19321 tgcagttctg agatcaattc ctctaactct tctacataag gtccttttcc tacgataact
    19381 aatctggcgt taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata
    19441 tttttctcat aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct
    19501 agtgactccc ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca
    19561 atgtcgatac cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta
    19621 tcaataacac gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac
    19681 aagtatttta catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct
    19741 tcatacatcg tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc
    19801 ccaagtatcc ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg
    19861 gcaaccaaat aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata
    19921 aatggtacac ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc
    19981 gttgtaaaaa tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc
    20041 gatgtagcaa ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg
    20101 tttttcgcct ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt
    20161 ttcattcagt acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg
    20221 ctcttttgct gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg
    20281 ttggatcagt cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc
    20341 taaccagcca ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc
    20401 ttctaaaaca acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc
    20461 tgaatacgca ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg
    20521 atgatcttct tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc
    20581 aaaccaaata aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc
    20641 tttacgttcg aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa
    20701 atattctctg aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc
    20761 attggaaata gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata
    20821 aggtgttggc gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac
    20881 gataggagaa agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg
    20941 cgcatgatag acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt
    21001 gattccgtat gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga
    21061 gataccgact tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt
    21121 tgcaagcatc ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact
    21181 tatatagtat aacatagcct acaaactgtc actcaaactt aagccctatt ttt
//
LOCUS       DANJJH010000046        21114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000046 DANJJH010000000
VERSION     DANJJH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(43..1506)
                     /locus_tag="PWS53_001591"
     CDS             complement(43..1506)
                     /locus_tag="PWS53_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0871821.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1570..4587)
                     /locus_tag="PWS53_001592"
     CDS             complement(1570..4587)
                     /locus_tag="PWS53_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0871822.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4738..5577)
                     /locus_tag="PWS53_001593"
     CDS             complement(4738..5577)
                     /locus_tag="PWS53_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0871823.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5722..5967
                     /locus_tag="PWS53_001594"
     CDS             5722..5967
                     /locus_tag="PWS53_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871824.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6030..6452
                     /locus_tag="PWS53_001595"
     CDS             6030..6452
                     /locus_tag="PWS53_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871825.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6546..6863)
                     /locus_tag="PWS53_001596"
     CDS             complement(6546..6863)
                     /locus_tag="PWS53_001596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871826.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6890..7096)
                     /locus_tag="PWS53_001597"
     CDS             complement(6890..7096)
                     /locus_tag="PWS53_001597"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871827.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7077..7259)
                     /locus_tag="PWS53_001598"
     CDS             complement(7077..7259)
                     /locus_tag="PWS53_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871828.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7273..7623)
                     /locus_tag="PWS53_001599"
     CDS             complement(7273..7623)
                     /locus_tag="PWS53_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871829.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7649..7951)
                     /locus_tag="PWS53_001600"
     CDS             complement(7649..7951)
                     /locus_tag="PWS53_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871830.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(7954..8187)
                     /locus_tag="PWS53_001601"
     CDS             complement(7954..8187)
                     /locus_tag="PWS53_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871831.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9173..9495)
                     /locus_tag="PWS53_001602"
                     /pseudo
     CDS             complement(9173..9495)
                     /locus_tag="PWS53_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9618..10733)
                     /locus_tag="PWS53_001603"
     CDS             complement(9618..10733)
                     /locus_tag="PWS53_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0871832.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10739..11137)
                     /locus_tag="PWS53_001604"
     CDS             complement(10739..11137)
                     /locus_tag="PWS53_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0871833.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12166..12552)
                     /locus_tag="PWS53_001605"
     CDS             complement(12166..12552)
                     /locus_tag="PWS53_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0871834.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12549..13070)
                     /locus_tag="PWS53_001606"
     CDS             complement(12549..13070)
                     /locus_tag="PWS53_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0871835.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13072..14106)
                     /gene="sppA"
                     /locus_tag="PWS53_001607"
     CDS             complement(13072..14106)
                     /gene="sppA"
                     /locus_tag="PWS53_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL0871836.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14139..15554)
                     /locus_tag="PWS53_001608"
     CDS             complement(14139..15554)
                     /locus_tag="PWS53_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDL0871837.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15523..17676)
                     /locus_tag="PWS53_001609"
     CDS             complement(15523..17676)
                     /locus_tag="PWS53_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDL0871838.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            17934..18170
                     /locus_tag="PWS53_001610"
     CDS             17934..18170
                     /locus_tag="PWS53_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDL0871839.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18189..18404
                     /locus_tag="PWS53_001611"
     CDS             18189..18404
                     /locus_tag="PWS53_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDL0871840.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19107..19859)
                     /locus_tag="PWS53_001612"
     CDS             complement(19107..19859)
                     /locus_tag="PWS53_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871841.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(19874..>21114)
                     /locus_tag="PWS53_001613"
     CDS             complement(19874..>21114)
                     /locus_tag="PWS53_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0871842.1"
                     /translation="GNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVSAAI
                     PIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGYVVF
                     QIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQVFD
                     HFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQEKF
                     ARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKNEYL
                     KSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRE
                     LSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPN
                     MFVNYQNDPYQFTTQVILLF"
BASE COUNT         6185 a         4055 c         3121 g         7753 t
ORIGIN      
        1 cacattttct ataccactcc ttatcagaaa cagccttatt ctttagaata agactgtttc
       61 tgggttgatt aatttgtctt ttgttaaact accatctaag gcaagttcta aatgtaaatg
      121 tggtcctgta ctatttcctg ttgaacccac ataaccaata atttgtcctt gttcaactgt
      181 ttgtcctacg ttcacgcaat attcctgttg gtgcgcataa agagccacac ttccgtcttc
      241 atgctcaatt gtgacataat ttcctgacaa agtatgatac gaagcttcta tgaccgttcc
      301 tgccttgctc gcatagattg gttctccttg attggctgcc atgtctaatc ctctatgaaa
      361 ttcttctcct cgccaaccga aggaactaga aattgaataa ttttttagag gtaccatata
      421 gccttctttg cttagttgtg gaccagctac ttctttatca tatttgatta agtcataggt
      481 ttcgattaac ccatttaatt ttttatcgta gctagtgtct gttgcgtatc ttcctgttaa
      541 aaatttagtc gcttcctgat aggtttttgc cttacttttc caaacaccat cataaaaaga
      601 ggtatttcca actaagcctt cttttaacag tttggcatag tcttttaagc tttcttcata
      661 gctctcatat tgccgaaaag ttgcttgggt cgtataaagt gtcccattgc ctaaatcttc
      721 ttgtgtcgca aaagagacgc cctttccgtt atgtgtgcct ttgattccaa atagattgta
      781 gtttggtgcc tgagccaatt gactttgtcc gcttgcggat tctaaaatgg cttgcgcgat
      841 cataacagaa gcgtataaat cattttcttg tccaatttta cgagcagatt caccgatttt
      901 acggataaac gtttctacag acgcgtcttt ttcaaaatga atagtccctt cgttatcttg
      961 tgtgctggaa gggactaatt cttgtatcgg tgcgcttggt gcttggtggt agttttttgt
     1021 ctcttctgaa ccgattgatt gtgcctgcgg tacgtcattc ataggtacag tcacactctg
     1081 ttcaggtgcc ttttgtgggc gttccgtttt tgttggcggt tccgcttctg tttcggtact
     1141 tggtttttga ggagtctgtg agctttcatc agaaggttgc tcatttggtt cttctgacgg
     1201 ttcctcctca ggttgttgat tgtcttctgg tttgtctggt tgattcggtg tttctggttg
     1261 ttcttcagaa cctgttggtg gagcgttcat ttctggatta tctggtgtat ctattgtatc
     1321 ttcagtcggt gtttctggtt gattttcttc tccagtggaa tttgttgtcg tttcttcagt
     1381 gatcgttgca gattgcgaat ttgtcagttg attgttgcct tgttcattag ccaaaaccgc
     1441 tggcgtatac ccaaatactt gacctactaa taacaaagca gataaacatt tactcgtttt
     1501 tttcacaaga ttgcctccat tcttcaatca atcatctaga gacagctgat tgctgtctct
     1561 agtcatactt taattgcttt gtttcatttt acgacgaacc agaacagccc ccacaccaac
     1621 aaaaagaaac agtcccaatg caattaacac atcgttcact gtttctcctg tttttggtaa
     1681 gctttgtttt ggtgtgctcg tcggcgtttg tttgacaggg gttgtcggcg tttctttttt
     1741 aatgcctttt gctgtcaatg tttgtttttc attcttcaaa tcaaagtgac gtgcttcttc
     1801 ctctttatgg tctttatcct tatagacaat atgagttgcg accaatttct ccccgtcttt
     1861 taacgtgcct gctttgtagt caaaggatac tttaaactct tcagacgctt tagaaacttt
     1921 acgtgcagtt tctaccgtac caacgactgt atcttttcca tttttatcaa tcttatggaa
     1981 ttgagtcacc cagtatttgg tttcccctac cgtagattct ggaaattctt gcgatacatg
     2041 atcgatcaac tcttggtctt tgtctggatc aaatgtttgg tcgccattca ctgtaataaa
     2101 gcgtgtttcg atttttggtt ctttcgcagt cactgtttga tctttattgg ttaaatcaaa
     2161 atgacgagcg aattcttctt cctcttcttt gtcgttatag acaatgtgcg ttgcgacaat
     2221 cgtgtcaccg tctgaaacca tgtctgcttt gtagttgaac gtcacatcaa atgtttcttt
     2281 ttcatcggtc acttttcgtg tttcttttac cgtatctaat acagtgactt ttccatcggc
     2341 agtaatttta tggaattgcg tcacccaata tttctctgtc ccaaccgcag atttagggaa
     2401 tacttgggac actttatcca ctaattcttg gtcttttttc gggttgaaat cttttccacc
     2461 tgtcgttgtc acaaacaaag tagaaatttc tggttggtct tcttctggaa ccacgatttc
     2521 ttcgtttttc acagtaattg gttgtacttc tccgtctttg accgttacta aaatcgtttc
     2581 tgtttgtcct gacgcttgcc cataatgctc agggaacaca atttccgtaa atttatacgt
     2641 accgtctggt aattttagtt tttctggttc ttcttttgtt tcatacacaa aggacacatt
     2701 tttattatgt gcagtcatac cttcaatcag gaatttcgct cctggtaatt gtagatgatt
     2761 gattgaatcg actttattga agtctacttc acccgttttt aacaagttgt caatcacatc
     2821 taattcaaac gtaagcacag gcgtcgtgtt atcgccatag gccacagaga acgtggtttg
     2881 ttcttcagaa ggttgacggt agttgtctgg cacatttttt tcaatcaatt ggtatttgtt
     2941 ttgatcgcca ttgtagaatt ttttgtttgc cttccagtgt ccgtctttgt cagtttttac
     3001 tgaatccaag acttctcctt ttttcacaag gactgtttct ttatctggcg cagtaatatc
     3061 tgttttggcg ataatatcaa attcgatatt ttctgcagga acggtatcat aaacaggttt
     3121 gccttgattc caggattctt ccgcattata gacttctttg actttctttc cttctggaat
     3181 gaactgttgt aagtcatttt ttgcagttaa cgtcacttct ttgtttggtt caatcgtaat
     3241 cgtatgagtt tgtttgtcta gaatataccc agctggtgct ttgacttctt tgacatccaa
     3301 tttattttga acatattttc cgtctttttc atagaatgca gggatttctt ttaaggcgat
     3361 ttcccctttg gcatccgttg tatattgttt tgtttctccc gtatcatgac gtttgatttc
     3421 atacgtcaca tttggaactt ttttccctgt gacagcgtct agtttggtta gtttgaagtc
     3481 gccatttttt agaactttaa tagttagtcc agctctatta ggatctggca cataaagctt
     3541 tcctagattt tgagcaacag gatctacata ggcaaaactt gaacctaccc ctctagaatt
     3601 tggatccagt gaagaaacag aaattcgtcc gtcatttgag ttttgatttg cttttataaa
     3661 caaactattt cctgattttc ttacttgaac gttagctgtg ttttcaacaa ccgacccatc
     3721 ccattgagaa aacacacctt ttgtatcctt aacttctact tcttccccta ctttaaccgt
     3781 cactgtttgg ctatcaaaag aaggatcttc aagtaaattg tttatttttc tatctacagt
     3841 ctgtttgaaa gcttcataat tagattgtcc aatagttcca taattaacaa atgaaccacc
     3901 taaaacttcc caaatatatg tttgtgctaa tacatagtta tccattgaac cactagaata
     3961 gtagccaaaa taaccaatta aagaaatctt ttttctaata gcacttgtcg taattaaatt
     4021 ttcgagtggt gtcacatcat attgaatacc cgcactcgtg attgcgcctt cttgaatgca
     4081 aaaaacatct tctccgtcaa tttgccagcg aaccatgtaa tcatgagtat acattgttcc
     4141 ttcaggagta attcgagtca tattcacatc tttacgacca ttttcagtca atgttagatt
     4201 ggtagcacgt gtctgtgcat tcttttccaa gattgcttgc cattcttcag attcttgcgc
     4261 caatcgttct tgttcagaaa aagaagtcgc tttttgaata tcttcaatgg ttataaagta
     4321 tttctcataa ccttcaggga cctcttgagt cattacattg ggcggagaaa catcttctgt
     4381 cgtgtcctgc gtagttgtct cttctgttgt ttcacttgtg gtttcagtac tgatcgcttc
     4441 ttcactactt tttgattctt cagtcttcat tacttcttca gtagaatttg attcatttat
     4501 cgtttctgca aatgccgtta cagatcctaa catgggattg agaaatgtaa cgaaaattgc
     4561 caaccaagtt gccattcttt taaacatatt tttttcgttc ctttcatttt atttttcagt
     4621 cttctaaagt aatttcttat ggacttcgac accgaaacat ttttatcctc ctcaaaacaa
     4681 aaaaagacta gaacttataa ttctagtctt catcaaccac ttacttttct tttattttta
     4741 gtagaaatct acagtgtgtt tttgtgaccc gtcactgtaa ataataacat ccaccataaa
     4801 acctttgttg taccactgcg aatttggatc gctaacctct gcattagccc aatcataggc
     4861 ttcatctaca gtgttaaata cctttccact attaccaatc tctccaacta caattgttgg
     4921 ctcttcaggt gtcgttgtgc caccatttcc gccttgatcg cctccaccag gattgcttgg
     4981 ttcttcaggc gttgttgttc ctccacctgt gcttgggtta gaaggagttg tttctcctcc
     5041 agtatttcca ccattacctg gttgtgtagg tgtttgagta gtgccaccat tgttttctgg
     5101 tgtacttggc gttgttgtac caccagttgt tcctgtttga tctccagctg gtgttgttgg
     5161 tgtcgttgta tttggtgtcg tttgtgtatt tgaagtcgtt ccttgtccta ctggttgatt
     5221 cggatcaact ttgtctttct cttgaatcac tgtttgagcc acagtttcac ctgcgctgtt
     5281 ttgtacactt gctacattgc cattaaatac taatttattt cctgcataga ttaaatccac
     5341 atttgtaatc ttattcatgt ccgctaattt ttggacagta atatttgttg cttgactgat
     5401 tgcgcttagg gtatctcccc agacaattgt gtattcattc cctttaattt ctgctttgat
     5461 ttgttccgcc gaacgaggtg tccaaacttt gttttctgct gcttcagcat tcgttcccac
     5521 agcaagacta actcctccga ctacgattcc tagtgccaat actttcaaca ttttcattgt
     5581 ttttcgcctc cgatttgaat aaatttattt acaaaaacat agtaccaaac ttatgtctat
     5641 cagtcgatat attttttgta gattttttta ttttttgata tactaacttt ggtactaaaa
     5701 taactaaaag gaatgtcaca catgcaactt aataatttaa atgaaataca tgattgggct
     5761 caaaataatt tacttaatcg taaacaagct gccaaaatca ctggacaaaa ctatgatgct
     5821 ttttctagct ctttacgtag aaataatatc aaaccatttc tagaaatagg cgacgaacct
     5881 ggtccttcca tcattcgctt atatctaaga gaagatattg aaaaatacgc aaaacagctc
     5941 gaagaaaaaa gacagaaacg tcaatgaggc gttttctgtc ttggatttac aagtaaaaca
     6001 ataataaaca cttttacaac ggaggttcta tgaataagaa atacattgtt tcagttggtt
     6061 caatcgtact gattgtactc gctattattg ctttttctgt atccaacaaa acagaaacac
     6121 agccacaaaa tacggttcaa tcgactacta caagtcaatc tagcgatcat tccgtctatc
     6181 cttcactaaa tagtttaaaa gaaacgcatg gacaagattt aattgatctt ggttcttctc
     6241 aaattactac tgtcttattt gaaaagaaaa atattgcgtt tattactcct aaagaaggaa
     6301 ctgaccttga attttctatc aaagtaacaa ataccaaaga aaatttattc tccattcctg
     6361 acattcaata tacccctaac aagttaaaga ttggggatac gtttggtttg gctaaagtaa
     6421 atgaacaata ctttgcttac tcactgaaat aagtttctaa tagcaagagg aaaaaccctc
     6481 ttgttatttt tatcacataa tcattttata aaaacgcttc gaaaatatca acatttttga
     6541 agaaactatt tttctaattt ctcaatttct tccaagggat tttcgctaat gacccataca
     6601 ggcacagatt gctctcgagg ctccccttct tctaaccacc aacctgcata atgaacttct
     6661 tcctcctcag cattaccatg ataccaacca acaaaggatt catctacaac gaatgaaatt
     6721 tcttcttctg gatcaaataa ttcatttttc catgcttcaa ttgcttgttc tttggtgtac
     6781 ttttgtttgt taaatacacc acaaatttcg tcatctacat aaaaaatttc aaaatcaaaa
     6841 ttactcgttc cttttactgg catcttaact tttcctcctt tgactcactt caatagatat
     6901 accactcatg ttcattccat tgtttactag ttaattggag ttttatctta gggctttgtg
     6961 cactctttcc aaaaacttga gcgtcgtttt tttcttctaa ccaaaaaagg taatttctta
     7021 cattcggtac taaatgtttt tcctgcattt cttcatgaaa aacatataat tctgcttcat
     7081 ttttcaacca attcattatt cattccctca ttcacttctt cataccgcca ttcagatagt
     7141 gagatttctt tttgtttctc cacttgtcct ataaaaatcg ctgtattcaa caagctgatc
     7201 acacttaata ataaagcaat gaaactaatg gttacactta ctctttcaca ttttgtcatt
     7261 ttgtttcctt ctttactcgt ttgttatttc ttgtaatagg tctaataact ccttcaccga
     7321 aatttgcaaa tggttcgcaa aaatttcaat aaaagcactc gcacacatta ccagaccttc
     7381 aactgatata tcactgcgac aataaaattc tggagaaaca ctgatttctg attctttctt
     7441 acaattgaac gaaaaaacta tttttgaatc aaattccggt aattcttcca caactatgtt
     7501 ttgggattct accaagaaag ggagtgcctg ttctaatgct tcttctggtg tttgagcaga
     7561 tagaataatt gcttcttgtt cttttatttc attgttctca aatactttat aacttttttt
     7621 cattgttagt tcctctttca ttcctctctc aattagctaa gccgttactg gtacgccatt
     7681 ctctaaattt agtatcgcta atccttaatg ctcgtcgaat atcaatcatt cgataaccta
     7741 ttgcacgaag ttctttatat cttttagcag ttagcgtctc taacgtcatt tcaggcaatg
     7801 ggttcgaatc aaaaatagaa ttcgaaccga ttatttgggc caattgttgt acttgtaaca
     7861 gctcttctga acttaattca gtgattgctc gattgcctat cattgcatac attttctgtc
     7921 gaatatactt cttttcttgt ttagacaaca tttctatcca ctcctatttt cattatcatt
     7981 tgttacctcc acttcagtaa atccacaaat aaatgaattc atattccaag taccattccc
     8041 ttcaactaca acttttctcc tgtcctcttt cggatattca agaatcagac cgtttaccaa
     8101 tatgtttttc actactacta atttgtttgt ataccgtgga attgctgttc cgatatactt
     8161 cactttattt cctacgctaa ttgtcatttc ctcactccta ttctttttta tccaaaactt
     8221 tttcctgtcc tattcctgcc aacgaatata cttcactgca tgcttaaaca aaacaaaatg
     8281 ttgccctgtt ttatttccgt cttttacttg ttccacatac agttcatatt tggtcacacc
     8341 ttttaaaacg ccagatactt tttttcatcc attgtcacaa ttagtttttt catcaatcga
     8401 ttgatactgc actgctcatt tttcttgttc atatagtatt cctttctttt tcaaaactag
     8461 cctcttttgt ttactagttt tcatcaatca tcaacgattt aacagatttt ttctttcgca
     8521 gggtttctag cgtcagcaac cccaaactct aaaaaagtgc ctttgctccc ttgaaaaacg
     8581 agcggacgat tgtactcgtc atcatatgtt tttcatacta cctgatccac gcaaataacc
     8641 tttttttgtt ctgtgccaca cccatccata cgtatcgctt gagattcacc atactttttt
     8701 aggtggttta tccttcctaa gtccctgtta gacacgctga ttaacttaga aagtcctgtt
     8761 tgtatccaat tttcaaagat ctcccaaaag aaataagtag gtttacgtgt atcgcttctg
     8821 ttcaaagtac ctgtttgtac ttgagtattt ctactcccct cgttcaaggg attccttcca
     8881 ccagctagct tttcctttca tcatcaaatg ttcctgaaaa gcatttacgt ttcgtgtccg
     8941 tgtgcttact tattcttttt tcgatagcga ttatccgctc gctactcgct attttatact
     9001 ttgtttgtta gctcaaataa aaactaacaa acaaaaaagg ctagtttctt tccgttttat
     9061 ttttttaact gtcgataaat agttgttagt ttaattccaa gttctaaaac caatacgccc
     9121 aacaaaacca caggaaaaac aattggggca aacaagtaaa atcggatagt tttcatgtct
     9181 tccctctttc tttttggtgt cgctgtggtc gttcttgccg ttcaatactt cgatacattt
     9241 tttattcatt atttgctcgt tttgttttcc tctggactgc ttttcttgct ttttgaatac
     9301 tttatacgct tttcgtgcct gaattaatag gtcttgtcgg cgtttcattc gtgagaaaac
     9361 aatatttgaa accatcaaat cctggttcga acaaccacga ttcgcaataa ttttcccatc
     9421 aaaaaaaata gttttcactt gagaaaaaaa cagttggaaa tcttgttttg ttgctaattt
     9481 ttttgtcaaa atcactgtgc ttttccctgt tgaatctaca atgattcttc gaacaatccg
     9541 attcgataac ttccaacgtt gccgattaaa caatgttact tcacaacaaa tatgacgtaa
     9601 gtgattaggg gtcattttca tgtgtccaat tcctccaacc aattatgcat cgaaattttg
     9661 ggcaattgtg acagcccata aatctgcgtt tccaggtctt cataactaca agaacgcttg
     9721 gcttgaagta atgaatattc tgcttgatct gatagttttg ttgccattgg atctagattc
     9781 aattctctta cacaacattc ccaaaaattc aatagcgctc taaaataaaa tccttttcga
     9841 ttctgaattg cttttccttc atgacgagca attttttcat tttgaataca ccgttgatac
     9901 gttttgctta aatcttctgc aaatgtttcc cctaaaattc gatactcgct tccatcagat
     9961 aaaccatgtg tttgattata ctttcgttgc cgcttttcta taattcgttt actgtcgtat
    10021 atcatgttta gctgctcttt cgcttcacgc cattcagctg aactgcgaac aatccatgtc
    10081 aaaacatctt tcggaacaaa cgtactttcg ctgtaatgag caataaactc tttttgccaa
    10141 tcatcagcca tttcaacagg actagtcgta tcgatttttg tatctagatt ttctgtatct
    10201 agattgttag aagaattatt aaataattgt gcagaatttt cacatggatt tttttcgtca
    10261 aaatcatcga aaatcgctaa attatcaaca gattcctcct cttcttttcc ttgtgcagaa
    10321 tctacactgg ttgagtcaac aggacctaat gaatacacat cattttctgt cgtatctaat
    10381 tctcctagat acaaccgagc tttgtttctt tttcctgttt gttgatcaaa tcccatatcc
    10441 tctatttcta gtagctcata ttctttcaat tcttttttga ttgtattaac agaacgtctg
    10501 caacaattca atgcattggc taattcttgt tccgtaaatt caaaataaac aaaaccttga
    10561 ctatctactt gatttcgttg aatggctaaa tctaagcgtg accgaaaaac catatacgcg
    10621 atttttgctt tatcagacaa tgagcgatat ttttcacttg taataaatac ctttggaaat
    10681 tgatagtata attcattgta tttacgagtt gcggtaaatc tttttgttgc catcgttatt
    10741 acacctcatc ttgtccatat attaagaaag aattttggtg ttggtagaca cgaacatctt
    10801 ccattgtagc aatcaattct tttagataaa ttcgaccgaa ttcaattgtt agtaagtcgt
    10861 aatcttctaa ttcatttttg atttcttcaa tttcttcttc agacacattg agtaaaatgg
    10921 ctaattcttg attggtataa gaaatataga catgtttctt ttcatcaatc cgtttttcaa
    10981 ttaaggattc ttctaagtac tcattcagta agctataggc gtattttgct tgtgcagtca
    11041 tcgcataata ttttgacgat tttataaaga atagttttgg catatgaaaa gttgcttgtg
    11101 tcagcacatc tgttgcgcga aatttgtttt ttaccatatt ttttcctcct agaaaaacac
    11161 gcaaaaaaag ctctgtataa atcgaattac acagagtttc ttcttgttga tatttaattt
    11221 ctaaaatacg tttcgggtaa aaactaaaca cttactaata tttagattgt agaatcttcg
    11281 ccttcattta ttcattttaa agagaaaaag atcaagtgaa atgtgcccaa aagtttgatt
    11341 ctattaattt ttaaacacca ttcataatat atttcctata atgtaaaaag cgtcgatact
    11401 tattaaagca tcgacgttat ttttatagta aatttatcaa aatttttgcc aacatttttt
    11461 ccgatttttt tccctatttc tatctttcct tatctttctt agttaatatt atttgatgtt
    11521 aatatatcac tactaatatt aaaaaacatt gatttatcat aggataaaga gtgatagttt
    11581 aatacttaaa gagacatatt aattttattt aaaaattgac attaatgact cttaatcagc
    11641 gggtcgcggg ttcgagcccc tcacggccca ttgggtgcca aacccacgga acaggtaaat
    11701 gttggtgcga aagcatttcg aaaacattta cgctgttctt tttttatttt tcaaaataac
    11761 tagggctttt tatttttaac tcaaagtcca ctgtagccct ttggactttg agttaaaaaa
    11821 gaagcataat tatgaacagt aaagtttcta atataacaat aattcgagaa gctttgcaaa
    11881 atcatgctgc caatgaactc tctaaacata taggattaaa tctaagtact ataaaaggtt
    11941 aaaaagtgaa gaatatttaa tagaaaaact aaatttactt gctgctttcc ccttttccat
    12001 taatacaaca atcagagagt gtcttttttt cgaaaaaatt agaataaata aaagacacgt
    12061 agttttttta tctacatgtc ttccatcgac tacaagttat catttctcaa aacgtattta
    12121 gcttttaatt gattaatcgt ttcacgttgt ctacacattg cctaattaag attttcttat
    12181 tagaagcatc aatcccgcaa taatacctaa aactccagga attacccaca ataatgttaa
    12241 aagaaaaaat ggaataatag ataaaatgag taaactaata ccatagacca ctgttttttg
    12301 attttttctt actaaaaaac ctattatgaa aatcattatc gaaaaaacta gggtaattcc
    12361 tccaaataaa acaacattcc cgcttagatt gactaacata tctatatcct tactggttag
    12421 ttctgtagta tttaaagtag ttgaatcaat agaagataga aacataccac ccatcaatgc
    12481 taaaagacta cataaaatcc ctgttattcc actaattaaa atcaaaataa actctgtttt
    12541 tctcttattc atttaattct ctccagatta agtgttttag aattagaaat tttttgacca
    12601 tagttaatag cttctaatga acgaataaca accactgacg tgttagaaaa ataatcccca
    12661 atatgttgtt tcttgggcgt gaaagcagcc accaaatacc caatttgaaa tacaaaattt
    12721 tttaatataa acctacatat acactcacga ataaagatag tttgccaact tagctctttt
    12781 tcctcaaagc tcactactct tatcccaaaa atcattttcc caattgtttg tcctgaattc
    12841 agcttagtca aaagtacaaa ataggttaaa taaatagcta atgacgctat accataaaaa
    12901 gtaataatgg atttgtcaga ttccagtccc atgattttgt aaaagagacc tatagtagca
    12961 tgggtgatga tgctaataca aattaaatca ataagaaaag caaaaaatcg taaccaaaat
    13021 cccgcaaata cataattagg caaagtttca atgccactct tcttattcat tttatagccc
    13081 tccatagtaa tacattggtt ttggagtatc agaaaatcca agtttttcaa aaactgaaat
    13141 tacacgatct gatgtagttg cctttagccc ttgccattca gcaatcttat tacctaacca
    13201 agaagaagca aagccagttg tgtcattttt atattctata accgtcgcat cagttaattt
    13261 cttctctttt tttagggaat ctaatgcatc ttcagagtaa ccaatttgat ccaccaaccc
    13321 attttctact gcttggttac catcataaat tcttccatcc gcaagttctt tcactgcatt
    13381 agtatccata tttcttcctt ttgctacaac attgacaaaa cgatcataag cactcatcac
    13441 aaattcctgt ataactttat tctcttcctc tgaaggtttg tgttgaggtg acatcatatc
    13501 ttttaatgca ccacttttat acgtcgcatc agttacgcca agtttttcta ataagccaga
    13561 ataatttaaa ccagaaataa tgacaccaat tgatccagtt gttgtttctt ccgtagcaaa
    13621 tattttatct gaaccagcag aaatgtagta ccctccactt gccgctgtat ttttaaatgc
    13681 agtatagata ggaatatcta gctttttaat ttttgccatt tctttagcaa tctcagcact
    13741 ttcatatatt cctcccccag gtgaatttac ttctaataac actcccttaa ccgctttgtc
    13801 ttcttgaatt ttctttagct gtgttaaaaa attctgatgg ttatattgtt ccctactaaa
    13861 aagattgctt tcacctgtat ctgcaataac tccattgacg gatagtttga caatcttttt
    13921 atttgatgcg ccttcttcta acgtttcttc tgtgatttcg ttagatccgt aaaaaatctt
    13981 atttattcct gttagggttg tatttgatgc ttcctcttta tcttttcctg gaattagcgc
    14041 gacgataatg gatagtaaca ataatccgca cgctactccc acagcaatcc atcttctttt
    14101 attcatataa actcctccta tattcttagt aaaaataatt attttttatt tagaaacaca
    14161 tcgacaattt gttgccaata tgttttttct cctacaatta aagacaactc tccagttaat
    14221 ccataacgaa acggaaacga ttgagttgtt tttaacgttc ctttcactgc ataaaaaaca
    14281 cctttctcag tggcagtaga attttctgaa atttcatcta aagttccgtc gatcgtaatc
    14341 ggagccccac ctttttgatc caatttaaaa tgaaccttca tcccttcttt aatataggta
    14401 ctttcattcg caggtaaaag tgctgtaaat ttcatttttg atttttcgtg gatcggataa
    14461 atttctgcaa gcactgttcc tttggctagt tctgcttgtc cctttgtttc ttcatttaaa
    14521 tgaaccgttc cagatacagg agcttttagt attccttttg aaagttgatc gtccaaactt
    14581 ttgataactg actggttctt ctcttgtttt tcatttagct ctgtaactgt ttgttttaca
    14641 gttgttattg cttgttctag ctcttgcttt cctttttccg tttgactaat tctgtcattc
    14701 tcagaagtag ttggtttaac taatttagct ttttctgact gatactgttc tacttctttt
    14761 tttagttgct caatttgttc atttatgctt gctgaaattg tcagtttcac ttgatttttt
    14821 tgatcatcag aaacattatt tagttgttct tgccaattct catagtttgc catcatttct
    14881 tttgaaaaat cttttaatga ttgattatta ctccaagcta cctgtacttg ttgccattct
    14941 tgtaatttac tatctgaact ctcaatttgt ttttctagtt gtctttttgt cttttcgtat
    15001 acttctaatt gcttttgatc atttaattca ctttgcttca aagcgtagcg gagtgattct
    15061 ttttctgaca acatgctctt taattgatta ctgtagccaa aactgtcatc cgttgaaaac
    15121 aaattttttc ctttacttat actttcaata aatagctgag ccatctttat ttgttgagta
    15181 atacgttggt tttcttgttc tatctgagcc ttttcattct ctattaaaga acaatccaat
    15241 ctaaccaaca catcgtcctt atgaacaaat tgattttctt ttaggaagtt ttccattatt
    15301 ttagcttcta atggcacttg taacttctct attttttcgg ggttcaattg agccgttgtc
    15361 cgaataacaa cctctttttt agcaaaggtt aaaaacaatc caataataca taaaaaaaga
    15421 attacagggt ataacaccca acggtagaag ctatgatgtt gtttagtata gatactagaa
    15481 tgttcgaacc aattattatt ctgcataaat tgtttccctc tcctagattt cccataattt
    15541 ttgatagata ccatcggcaa aacgaagttc atcgtgcgtt cctttttcta ccaaatgccc
    15601 ttcatggaga actaaaattt gatcacatgc ttttgctatt ggtaaatgat gcgcaataaa
    15661 aataatcgtt ttttctttta aattgaggag ataattcata attgcatgtt ctaacaaggt
    15721 atctaatcca ctagttgctt catctaaaat taatatgtcc ggatttttta ataaagcccg
    15781 tgctaaggct aatcgttgtc gttgccctcc tgaaagatta tttccacctt cttccaacaa
    15841 tgtctcgtaa cgtaatggct gttgattaat aaattcattt agttggacta tctcacaaac
    15901 ttctattatt tgttcgaaac ttggtgggct atctaaaccg aatataagat tctcaatgat
    15961 tgttccacta aagaaaaatg actcttgagg caaatatgtc actcgatcgc gtaatatctg
    16021 aaaaggaatg tctaagtgat taatgtttcc ataatggatc gatccttctg atgcttcata
    16081 aaaacgaaca aataactttg caagagttga ttttccagaa ccactaactc caactatagc
    16141 cgttttactg tgcgaaggaa ttgtacatga aatttcgttc aacgttgggg ccttcatgct
    16201 atacgagaat gaaacgtttt ttacagtgat atcttgttga aataattgtt cagaaagcat
    16261 atattcaaaa gactggttcg atttttctga atcaatcgcg aaaatttcat ttaaacgatt
    16321 atttgcaact tgagccgttt gcattttcac ttgtaaatta ataatgtttt gtaaaggatc
    16381 tgtcaaaaat actagtaaag cattgtatgt gataagttga cctagactaa ttctattatc
    16441 cataacaaaa taagaaccta gccataaaat taatgcactg ctaattaatt gaatcaaatg
    16501 tttaattcct tgttgaatat tatcaagcgt aaccgtacga aatgattttt tcattaattt
    16561 aacaaattca tggtctatac ggttatatac tttctcttca ccgctatatg ctttaattgt
    16621 ttctattcct ttaagacttt ctatgatact agaatttaca gctgcaccgg ctgccatttc
    16681 ttccctgttc gatttttcat agcttttcac gaaagcgaat attgcaacta cataaaaagg
    16741 caaagagcat agagtgatta aaaacaacgt actattctga atagctaacg taaccccaac
    16801 aagcaacacc ataccaatat ctaaaaatat tgataacgta gcacttgcta aagcatcaat
    16861 aatcttactg gcatcaagaa aacgagaaat aatttcccct gattttctcg tagcaaaaaa
    16921 attcattggt aaagttaaca catgtttgaa atactgaagc ataattgcca tactcattcg
    16981 ctgtcctaaa ataataagaa gataactacg actatattca aacaataccc gaaataaata
    17041 gacagcgatc aagccaatgg aaattatatt taaagttgaa cgtgcttgat tagggatgaa
    17101 aaaatcaaga ataccttgaa aataatatga gcttcctatg ccaaaaaacg taatgaaaat
    17161 cgatgctaaa acgatgtgga atactaatcc tttttgtttc caaattatag gcaaaaaaga
    17221 acttaatcca gctacttttt ctttaatagg aatataagtt tctctaggca taggtaataa
    17281 aagaatacct gtccattctt tttgaaacgc ttcaatcgtt tttctatttt ttccattagc
    17341 gggatctgca atccatagta cgtttccctt cgttttataa attacaacgt agtgcatata
    17401 gctgttatca accaatatat gtgcaattaa tggtaagata agatctcttt ctttccagat
    17461 ctcactatct gcttgaatag ccatacaatc aaaagacaat ttttcaaatg tctttttcaa
    17521 tccaaatgct gaagtaccct ctaaatcagt cccagatagg tcccgtaatt tatgaatcgg
    17581 gatatcagtt ttaaaatatt ttaaaatcat cgaaaggcaa gcaacaccac aatctttttc
    17641 atcttgttgc tgcacaaaag gaaatgattt tatcacttat ctaacacaac tccttctaaa
    17701 aaatttttat agaaaaattc tcaatatagc tttaatacag tgaaaatatt gtctcataat
    17761 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
    17821 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
    17881 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
    17941 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
    18001 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
    18061 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
    18121 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
    18181 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
    18241 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
    18301 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
    18361 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
    18421 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
    18481 gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat aattatatta
    18541 aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg gtagagatat
    18601 ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat ttcggccgtt
    18661 acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc tgcaagttaa
    18721 atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca taggattgca
    18781 cttgacagga caaacttttt aactaaaaaa tgaaaaacac tttctaaaaa ttagtagaat
    18841 ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt tatggtactt
    18901 aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata
    18961 ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt
    19021 acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa
    19081 atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt
    19141 ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt
    19201 tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt
    19261 gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca
    19321 tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca
    19381 tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc
    19441 tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga
    19501 tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt
    19561 gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat
    19621 tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag
    19681 aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt
    19741 tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt
    19801 cgttctaact gatttaattg tataatttga tcttcacata atattattgg ataactcata
    19861 aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt
    19921 gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta
    19981 acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct atagagagta
    20041 gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag
    20101 ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa
    20161 ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac
    20221 attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct atcaacaaac
    20281 ttttcaaata ttcatttttt atatttttta aataacgata ttcaaactta ttggttagat
    20341 aagaattaga atagatctct aattctttta tttctttttc caatatcgaa tcatggtttt
    20401 gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta aatctggcaa
    20461 atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc aattcttttc
    20521 ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata aataaaaata
    20581 gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa tgatcaaata
    20641 cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa taaatcatga
    20701 atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct aattttctat
    20761 ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat actacataac
    20821 catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa gtaataatag
    20881 atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact atattagttt
    20941 tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg gaaacatcag
    21001 taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga tattttcttt
    21061 tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt ccca
//
LOCUS       DANJJH010000047        20860 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000047 DANJJH010000000
VERSION     DANJJH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..562
                     /locus_tag="PWS53_001614"
     CDS             <1..562
                     /locus_tag="PWS53_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0871843.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="PWS53_001615"
     CDS             762..1076
                     /locus_tag="PWS53_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0871844.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="PWS53_001616"
     CDS             1097..1474
                     /locus_tag="PWS53_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0871845.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="PWS53_001617"
     CDS             1484..2257
                     /locus_tag="PWS53_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871846.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="PWS53_001618"
     CDS             2279..3682
                     /locus_tag="PWS53_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0871847.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="PWS53_001619"
     CDS             3864..5048
                     /locus_tag="PWS53_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0871848.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="PWS53_001620"
     CDS             5045..5335
                     /locus_tag="PWS53_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871849.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="PWS53_001621"
     CDS             5332..5553
                     /locus_tag="PWS53_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871850.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="PWS53_001622"
     CDS             5595..6374
                     /locus_tag="PWS53_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL0871851.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="PWS53_001623"
     CDS             6441..6935
                     /locus_tag="PWS53_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0871852.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="PWS53_001624"
     CDS             6991..7629
                     /locus_tag="PWS53_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871853.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="PWS53_001625"
     CDS             7701..8096
                     /locus_tag="PWS53_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0871854.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="PWS53_001626"
     CDS             8080..10533
                     /locus_tag="PWS53_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0871855.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="PWS53_001627"
     CDS             10530..12557
                     /locus_tag="PWS53_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871856.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="PWS53_001628"
     CDS             12554..13576
                     /locus_tag="PWS53_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0871857.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="PWS53_001629"
     CDS             13593..14522
                     /locus_tag="PWS53_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0871858.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="PWS53_001630"
     CDS             14767..14883
                     /locus_tag="PWS53_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL0871859.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="PWS53_001631"
     CDS             14899..15702
                     /locus_tag="PWS53_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0871860.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="PWS53_001632"
     CDS             15937..16173
                     /locus_tag="PWS53_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871861.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="PWS53_001633"
     CDS             16180..18132
                     /locus_tag="PWS53_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0871862.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="PWS53_001634"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="PWS53_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0871863.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="PWS53_001635"
     CDS             19063..20103
                     /locus_tag="PWS53_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0871864.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="PWS53_001636"
     CDS             20201..20389
                     /locus_tag="PWS53_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL0871865.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="PWS53_001637"
     CDS             complement(20447..20800)
                     /locus_tag="PWS53_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0871866.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         7128 a         3446 c         4014 g         6272 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac
//
LOCUS       DANJJH010000048        20485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000048 DANJJH010000000
VERSION     DANJJH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="PWS53_001638"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="PWS53_001638"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL0871867.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="PWS53_001639"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="PWS53_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL0871868.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="PWS53_001640"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="PWS53_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL0871869.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="PWS53_001641"
     CDS             2857..3732
                     /locus_tag="PWS53_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL0871870.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWS53_001642"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="PWS53_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL0871871.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="PWS53_001643"
     CDS             4629..5063
                     /locus_tag="PWS53_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL0871872.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="PWS53_001644"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="PWS53_001644"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL0871873.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="PWS53_001645"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="PWS53_001645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL0871874.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="PWS53_001646"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="PWS53_001646"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL0871875.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="PWS53_001647"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="PWS53_001647"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL0871876.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="PWS53_001648"
     CDS             13334..14260
                     /locus_tag="PWS53_001648"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL0871877.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="PWS53_001649"
     CDS             14442..15335
                     /locus_tag="PWS53_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL0871878.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="PWS53_001650"
     CDS             15469..15594
                     /locus_tag="PWS53_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871879.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="PWS53_001651"
     CDS             complement(15728..16360)
                     /locus_tag="PWS53_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871880.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="PWS53_001652"
     CDS             complement(16508..17050)
                     /locus_tag="PWS53_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL0871881.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="PWS53_001653"
     CDS             17185..17574
                     /locus_tag="PWS53_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871882.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="PWS53_001654"
     CDS             17578..18342
                     /locus_tag="PWS53_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0871883.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="PWS53_001655"
     CDS             18471..19418
                     /locus_tag="PWS53_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871884.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="PWS53_001656"
     CDS             19548..20288
                     /locus_tag="PWS53_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871885.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DANJJH010000049        19887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000049 DANJJH010000000
VERSION     DANJJH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            20..574
                     /locus_tag="PWS53_001657"
     CDS             20..574
                     /locus_tag="PWS53_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0871886.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(747..1220)
                     /locus_tag="PWS53_001658"
     CDS             complement(747..1220)
                     /locus_tag="PWS53_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0871887.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1232..1768
                     /locus_tag="PWS53_001659"
     CDS             1232..1768
                     /locus_tag="PWS53_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0871888.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1808..2179
                     /locus_tag="PWS53_001660"
     CDS             1808..2179
                     /locus_tag="PWS53_001660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0871889.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2463..3734
                     /locus_tag="PWS53_001661"
     CDS             2463..3734
                     /locus_tag="PWS53_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0871890.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3979..5004
                     /locus_tag="PWS53_001662"
     CDS             3979..5004
                     /locus_tag="PWS53_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0871891.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5298..6407
                     /locus_tag="PWS53_001663"
     CDS             5298..6407
                     /locus_tag="PWS53_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL0871892.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6490..7386
                     /locus_tag="PWS53_001664"
                     /pseudo
     CDS             6490..7386
                     /locus_tag="PWS53_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7402..8226
                     /locus_tag="PWS53_001665"
     CDS             7402..8226
                     /locus_tag="PWS53_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871893.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8258..9568
                     /locus_tag="PWS53_001666"
     CDS             8258..9568
                     /locus_tag="PWS53_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL0871894.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9716..10975
                     /locus_tag="PWS53_001667"
     CDS             9716..10975
                     /locus_tag="PWS53_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0871895.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10985..11857
                     /locus_tag="PWS53_001668"
     CDS             10985..11857
                     /locus_tag="PWS53_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0871896.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11869..12699
                     /locus_tag="PWS53_001669"
     CDS             11869..12699
                     /locus_tag="PWS53_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0871897.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12739..14922
                     /locus_tag="PWS53_001670"
     CDS             12739..14922
                     /locus_tag="PWS53_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL0871898.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14983..16203
                     /locus_tag="PWS53_001671"
     CDS             14983..16203
                     /locus_tag="PWS53_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL0871899.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16200..17660
                     /gene="gtfA"
                     /locus_tag="PWS53_001672"
     CDS             16200..17660
                     /gene="gtfA"
                     /locus_tag="PWS53_001672"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL0871900.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17737..18843
                     /locus_tag="PWS53_001673"
                     /pseudo
     CDS             17737..18843
                     /locus_tag="PWS53_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19165..19374
                     /locus_tag="PWS53_001674"
     CDS             19165..19374
                     /locus_tag="PWS53_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0871901.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19367..19516
                     /locus_tag="PWS53_001675"
     CDS             19367..19516
                     /locus_tag="PWS53_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL0871902.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19670..>19887
                     /locus_tag="PWS53_001676"
     CDS             19670..>19887
                     /locus_tag="PWS53_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0871903.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEE"
BASE COUNT         6805 a         2987 c         3881 g         6214 t
ORIGIN      
        1 tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa atgaagagat
       61 acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata gttttttatt
      121 tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa aagttggaga
      181 tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc gaaaaccgat
      241 ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag aagaaaatga
      301 ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg tctatcagat
      361 atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata cattaagaaa
      421 aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa tgtttatttt
      481 taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag atgaaatagg
      541 agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata aacagtaaag
      601 acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt cgcggaataa
      661 aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt aattaaataa
      721 catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg
      781 caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact atctaatagg
      841 gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact tccttgcttt
      901 tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt ctgctttttc
      961 gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc aagtcgaaca
     1021 tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt atgacgaaaa
     1081 ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa attgtgattt
     1141 acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg ttcaccatct
     1201 tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc acatttctat
     1261 tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg gtttaaaatt
     1321 actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt tttcagataa
     1381 tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga acaaccagtt
     1441 ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag catcattgca
     1501 tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg aatcattttc
     1561 tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt tagttccctt
     1621 acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga tccctacaga
     1681 gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg atgcgactga
     1741 acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct tttcgggtca
     1801 tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag ttcagattat
     1861 tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc atgttgcgtt
     1921 tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag agcgcaattt
     1981 tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc gtgacccaaa
     2041 tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa
     2101 tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa atttcattcg
     2161 tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag aggttgactc
     2221 ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt gatttgatgc
     2281 ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga gtcagcctct
     2341 ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc aaaaataccg
     2401 tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa tgaacgagta
     2461 tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa
     2521 ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt gtttttgata
     2581 ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt acccgtttat
     2641 ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct acggcgattg
     2701 tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg attactaaca
     2761 tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta ggatatgcaa
     2821 atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga
     2881 cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct gattttgtat
     2941 tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca aagaatcatg
     3001 gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact attcagagaa
     3061 attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt ttggaattaa
     3121 ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa gagacattaa
     3181 gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac caagctagta
     3241 cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt ctttcttttg
     3301 actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat atcgatacat
     3361 taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct gaatttgagc
     3421 gaatttatca agaattagct gaatttactg caattgttgt tactggagat tatttagcgg
     3481 ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa ttatcgatta
     3541 ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca acaatacgac
     3601 taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg gccaaacaag
     3661 aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga gactctgttc
     3721 acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa tatccgaacg
     3781 atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag aattagcatt
     3841 atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac agtgcctatt
     3901 tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac actttcaaat
     3961 atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc
     4021 attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac cgatgaaacc
     4081 aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag tcccgttgca
     4141 aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga gttggaagat
     4201 ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag caaagtcaat
     4261 ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt gctaaaatgt
     4321 caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt
     4381 aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga tgcagtttat
     4441 actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa aggacaccga
     4501 aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa taaacacgtg
     4561 tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa aggattagaa
     4621 aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca ggcagcagat
     4681 ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt aggaaatgat
     4741 cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat tccagaagat
     4801 atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc tgcattaaat
     4861 acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt taatgaacga
     4921 ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat tattgttaga
     4981 gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt
     5041 gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt
     5101 gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa
     5161 tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt ggtgggggag
     5221 atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat actgaccaag
     5281 ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag
     5341 aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca acaacgggag
     5401 gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat atgtcacaga
     5461 atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg ggaaacttta
     5521 ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc cctaaagatg
     5581 cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt gtcgattatc
     5641 cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta acagatgaat
     5701 caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac tggccattta
     5761 tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt ttgaataccc
     5821 cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat catgcttcac
     5881 caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca ggtagattag
     5941 caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac tatctagttg
     6001 aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt acaaatggac
     6061 ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa ctaatggcat
     6121 ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca atcccagcac
     6181 taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg agtgcttttg
     6241 gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta cgagctgaac
     6301 aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt gaagatggaa
     6361 ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt agttaattat
     6421 acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa taccaaaaag
     6481 ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt gatatggggc
     6541 tatattttta tttttccaac ggcattagga ttaattttat taaatatatg gccagcaatt
     6601 caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga atgggtcgga
     6661 ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt attaaacaca
     6721 ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt ggtggcagtt
     6781 ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt tctaccagtc
     6841 gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa tgattatgga
     6901 ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt aacggatcca
     6961 tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg ttataacatg
     7021 gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc agcggaaatt
     7081 gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt tacaccaaca
     7141 ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga tgttattttt
     7201 atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc ctatttattc
     7261 tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt tatgttcctt
     7321 ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac
     7381 tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc aatccatatc
     7441 atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat tttaacttca
     7501 gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga gaaatttcag
     7561 ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta tatcaataca
     7621 tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat ggctgcatat
     7681 gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt acttattcaa
     7741 atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca aaaattgggt
     7801 attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt tggtacgttt
     7861 ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc ctatttagaa
     7921 ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa atcaggattg
     7981 gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg gccattgatt
     8041 gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt acaaggacaa
     8101 tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg gccaatagtt
     8161 gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac aggtagtaaa
     8221 ttataaaact attaattagt gattggagag taagtacatg acaaaaatta catttattgg
     8281 tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa ctccaagtct
     8341 acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt
     8401 aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg tttcttatta
     8461 tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc aagtgggtgg
     8521 ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt tgcgacaaac
     8581 tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac taccagtaat
     8641 gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat tgaactatac
     8701 aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta agactgttgg
     8761 tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag gaatagccga
     8821 tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata tggcattctt
     8881 gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt tagctgcaga
     8941 aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt ttggttacta
     9001 tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta aatcaaaata
     9061 cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc gttgtgaaca
     9121 acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg agttaacaca
     9181 tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag gaactccgtc
     9241 agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga cagatgtttg
     9301 tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt
     9361 gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac tgacagtaga
     9421 agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag atccacatct
     9481 atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa tcgaagcaca
     9541 tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga attttcaatt
     9601 ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta gtaaacagtt
     9661 acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc ataaaatgaa
     9721 gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct ctttgactgc
     9781 ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa
     9841 aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa aaaaccctga
     9901 tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct
     9961 tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat tgaaagaatg
    10021 ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga atatcaaaaa
    10081 tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat taaccgctaa
    10141 tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc aagcaccaga
    10201 aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt
    10261 tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag ctatagcgac
    10321 tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat
    10381 taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt taagagaacc
    10441 aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag ttacttttac
    10501 aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga ttcattcaca
    10561 aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag gtcaaagtct
    10621 aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac ataaagaaga
    10681 agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat attatgatgt
    10741 agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt caccattaag
    10801 tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca aagattggaa
    10861 tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca atcaaaaagc
    10921 catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa
    10981 taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg tagcaattcc
    11041 aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt tttacagttt
    11101 aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca attataagat
    11161 tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg tgacgctttg
    11221 catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga acgcaaatat
    11281 taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat cgggcgtggt
    11341 tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg gtagtatgtt
    11401 aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga tttttgcagt
    11461 aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct tagcgggttt
    11521 tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga atgataagca
    11581 aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg tatttattat
    11641 ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg gtggtggacc
    11701 aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta aaaacaatca
    11761 gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga ttgtttcact
    11821 ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat gaaaacatca
    11881 aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat gattttagtt
    11941 cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat gaataatttc
    12001 tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc ggatggaatt
    12061 ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc tgtgacagca
    12121 attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa agcatacgga
    12181 ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat tatgattccg
    12241 attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat cttactctat
    12301 ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa actaaatgtg
    12361 cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac aacttatttc
    12421 aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct taatgctcta
    12481 tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc taatatgtgg
    12541 acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta tggtccaagt
    12601 tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc tttccaaaga
    12661 agtattatag ctggaatgag caatggagca gtgaaataac caataaatta ttctcattat
    12721 aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt tcatttacaa
    12781 aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac gcatctttat
    12841 tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac agatcgttcc
    12901 ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac attaccatta
    12961 gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat taaattagga
    13021 aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa aggaaagccc
    13081 agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga aacgctgatt
    13141 attcaattga aagacgcgat tagtggattg actttacagt tgttctacac aatttatcaa
    13201 aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga aacagtggag
    13261 attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga gttaattcat
    13321 ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca tacagggata
    13381 aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt tgttgcttta
    13441 gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt agtttatagc
    13501 ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt cattatggga
    13561 attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca aacgccagaa
    13621 gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta tcatgaattg
    13681 tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac gttaatcaac
    13741 aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat
    13801 gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa
    13861 aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg
    13921 gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg gtttgaaccg
    13981 gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc attgggtatt
    14041 ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc aagacaagat
    14101 gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc gattgattat
    14161 gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg
    14221 atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt agaaaagtta
    14281 acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg tcgttttgat
    14341 gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga tgccgtggct
    14401 cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat gggtgcgcat
    14461 gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat tcgtggcaat
    14521 gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac ttctgaagaa
    14581 aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact tttgcaatat
    14641 ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg gatgtttgtc
    14701 tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga agctgcgcat
    14761 ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca aatggaagat
    14821 gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc agaactacat
    14881 ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga atttacaata
    14941 taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta tttacacgca
    15001 tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga agaaatctat
    15061 gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga tgcgatatta
    15121 aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga tgggttagcc
    15181 ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa agaaatgaga
    15241 gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt tcctaatgaa
    15301 cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga ttttagttat
    15361 acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg gacatggacg
    15421 gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg ttggggcgcc
    15481 ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc agaagaatgg
    15541 atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag aggtacccct
    15601 tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc attagaagat
    15661 tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg attggcacca
    15721 gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac acctatgcaa
    15781 tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt aaaagtaaat
    15841 cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc ggtattcaat
    15901 tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt gctaatctac
    15961 ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga acgccaatta
    16021 gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga atattatgta
    16081 gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt agatcaaagt
    16141 cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg aggacaatta
    16201 tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga aaaaacatgg
    16261 gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt gtacatctgt
    16321 tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat acgacagttg
    16381 atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat tatttgatgt
    16441 ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa
    16501 accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa
    16561 atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa gcgccgtttc
    16621 aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt ggggaagaac
    16681 aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa accatcaaag
    16741 atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat gcgattaaaa
    16801 aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta gacgaagtgc
    16861 gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag cattattcca
    16921 ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg ccaatggtca
    16981 ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg aaaatgagtc
    17041 cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg gacgctcgtg
    17101 atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa gtcggggcga
    17161 atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat caaattaaca
    17221 gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca cgtgcgattc
    17281 aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca ggaaaaaatg
    17341 atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat tactatgatt
    17401 tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt gcacttttaa
    17461 aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta gcaacgccat
    17521 cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa
    17581 cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa attttcacta
    17641 gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata ctagcacttg
    17701 gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa aacaaataca
    17761 gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa
    17821 tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc
    17881 tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac agctcttatc
    17941 gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca attgtctcga
    18001 gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact gttagaaaaa
    18061 gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga ttccagtacg
    18121 ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc
    18181 aagttacact taaactattg ttatatggac gatactacga tgtatccaac tgattttacc
    18241 ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa tcaaccagag
    18301 gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca gatgcattgg
    18361 gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt gatagatact
    18421 ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt aggatctaaa
    18481 acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg acgtgtgttt
    18541 caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat gtatcacgct
    18601 cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat taaaaccttc
    18661 ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat ttccgctctc
    18721 ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca aatcaaacga
    18781 ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt aattcccaca
    18841 tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca gataaaaaat
    18901 tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa
    18961 atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt ttagaattag
    19021 atttctaatg tgcatttttt gagtattaag gagcaagtat acaaagattt ataaagctga
    19081 ttaaatttga ataaaaatat gcaggaacgc tactattttt agtagtgttc ttttttatat
    19141 ttgaataaaa aatgtggtgg ttaaatggtg gttataacaa aaaatgaatt aatgaaatta
    19201 ggttatggga agtatcagtc tgagagaatc atacgacagg caaaagcaat tatggtttca
    19261 aaaagctata cctattacga aaatagaaga ttaggactag tacctgttga atctgtagaa
    19321 gaagttttag gtatgaagat agaggtagag gaggattttc agaatcatgg ttaattttaa
    19381 aaaagataaa aatgggaaat atcattttac tgctagtttg ggattcgaca aacgaacagg
    19441 gaaaagagta caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa aaagcgtata
    19501 ccgaacttgt ttctaattat ggaaaagaag cagaaacttc tcaaagtact atgttgtttg
    19561 aggagttttt ttataaatta ttcctacctt attatcaagg gaaagtagaa gaaagaacat
    19621 ataaaaatcg gttatctaca atcaaaaaaa cttttcttat ttctttaaaa tggagttaag
    19681 agaaatacgt ccaattgaca ttcaaaagtg gcaaaataat ttattgagca aatataaaaa
    19741 ttcttatgta agaaacatgt atgggatttt tcagatgtca ttagatcgag cagttgtttt
    19801 gggactactt gaaaaaaatc ctgcaaaagt tattggaaat gtgaagaaaa ggaaaactac
    19861 agttgatttt tggacaaaag aagaatt
//
LOCUS       DANJJH010000050        19787 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000050 DANJJH010000000
VERSION     DANJJH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..161
                     /locus_tag="PWS53_001677"
     CDS             <1..161
                     /locus_tag="PWS53_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871904.1"
                     /translation="KTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINY
                     QPLAETHA"
     gene            complement(805..1758)
                     /locus_tag="PWS53_001678"
     CDS             complement(805..1758)
                     /locus_tag="PWS53_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871905.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1781..2536)
                     /locus_tag="PWS53_001679"
     CDS             complement(1781..2536)
                     /locus_tag="PWS53_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0871906.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2533..3492)
                     /locus_tag="PWS53_001680"
     CDS             complement(2533..3492)
                     /locus_tag="PWS53_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0871907.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3485..4435)
                     /locus_tag="PWS53_001681"
     CDS             complement(3485..4435)
                     /locus_tag="PWS53_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0871908.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4585..5037
                     /locus_tag="PWS53_001682"
     CDS             4585..5037
                     /locus_tag="PWS53_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL0871909.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5126..7393)
                     /gene="gshAB"
                     /locus_tag="PWS53_001683"
     CDS             complement(5126..7393)
                     /gene="gshAB"
                     /locus_tag="PWS53_001683"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL0871910.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7580..8578)
                     /gene="ftsY"
                     /locus_tag="PWS53_001684"
     CDS             complement(7580..8578)
                     /gene="ftsY"
                     /locus_tag="PWS53_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL0871911.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8589..9398)
                     /locus_tag="PWS53_001685"
     CDS             complement(8589..9398)
                     /locus_tag="PWS53_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0871912.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9407..12988)
                     /gene="smc"
                     /locus_tag="PWS53_001686"
     CDS             complement(9407..12988)
                     /gene="smc"
                     /locus_tag="PWS53_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL0871913.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13008..13694)
                     /locus_tag="PWS53_001687"
     CDS             complement(13008..13694)
                     /locus_tag="PWS53_001687"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL0871914.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14042..15823)
                     /locus_tag="PWS53_001688"
     CDS             complement(14042..15823)
                     /locus_tag="PWS53_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0871915.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15849..16763)
                     /locus_tag="PWS53_001689"
     CDS             complement(15849..16763)
                     /locus_tag="PWS53_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871916.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16790..17752)
                     /locus_tag="PWS53_001690"
     CDS             complement(16790..17752)
                     /locus_tag="PWS53_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871917.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17752..18696)
                     /locus_tag="PWS53_001691"
     CDS             complement(17752..18696)
                     /locus_tag="PWS53_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871918.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18697..19701)
                     /locus_tag="PWS53_001692"
     CDS             complement(18697..19701)
                     /locus_tag="PWS53_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0871919.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5477 a         4257 c         3524 g         6529 t
ORIGIN      
        1 aaaaaacgaa tagttttgga ttgatattaa gcgagtataa aatcaaagca gaagagagtt
       61 atttctatca attattaaaa cggtggagta agcttcccgc cgatttttat cgaaaaatgc
      121 agatcatcaa ctatcaaccg cttgctgaga cacatgccta aatagaggtt gtgaaatcag
      181 tgctttgagc taataccgaa ggtcagctga ttgaacattg tttagaagag attgtgacaa
      241 ctgcgatttg ctttgagtaa taagaacgct ggacagaaaa tagcttctca tattttctag
      301 tctagtgggc ttattgccga gaagcagcag atggaacatc gtttagaaga gattgtgaca
      361 actgcgattt gctttgagta ataagaacgc tggacagaaa atagcttctc atattttcta
      421 gtctagtgga cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt
      481 acaaaataaa aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca
      541 aatcaaagga ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc
      601 attctagaat caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa
      661 ataaataaaa accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt
      721 ctttgaggat aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga
      781 gagtttaatc ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag
      841 tttcattgat tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat
      901 ctgctgattt ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt
      961 gctgtcgtct ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt
     1021 ttccagcaca tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc
     1081 tgcttgttca aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc
     1141 tgataattgc ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt
     1201 ctttttcgtc tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg
     1261 cttaccaaag atctccccta aagcagtgac attttgctgg aaagattccc atggattttt
     1321 cgcatctaaa gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct
     1381 ttgtcggcca gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg
     1441 ttcttttatt ccacctgctg attctacttt tttataggat gaaagatact caggaaggct
     1501 gcttgtagct gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga
     1561 accattgtcg aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt
     1621 actatctgtt atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg
     1681 actggtgttg ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc
     1741 tactactaat tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc
     1801 tgcggccttc aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt
     1861 tttttatcat ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa
     1921 tttcgtctgc ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc
     1981 cgatttcttg tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta
     2041 gattgttcaa aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa
     2101 tatacgcacg ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt
     2161 ctaacccaag gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc
     2221 ctttcgtata aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta
     2281 tttcattcga ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct
     2341 ttacttcgtt atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca
     2401 tcgacaacac cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt
     2461 caggaatcgt taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct
     2521 tttgtaaatc aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat
     2581 tcctccgaca aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac
     2641 tgcgagctgt cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg
     2701 accatgacga taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa
     2761 agtaactgga cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat
     2821 caaaaattca aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg
     2881 gaaaacgtct agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa
     2941 agaagcaggc aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg
     3001 aagcaaatca tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa
     3061 aagcgtacct aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt
     3121 caaaagaaag aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa
     3181 aaaaactgta cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt
     3241 ttggacaagg acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg
     3301 cgtaattgtc tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc
     3361 aaaaattttc ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt
     3421 caagtacatt cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt
     3481 ttttttaagc atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga
     3541 ttccaacgat cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat
     3601 cgcaaaatac taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt
     3661 gatctccata tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc
     3721 ctaccatgat caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca
     3781 tcttctggta atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa
     3841 atcggtaagc aaatagccaa accaagagca acaatggaac aacagcatac aataactcat
     3901 aatttcctcg tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca
     3961 actggaattg ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc
     4021 caatcaacgg aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa
     4081 gcaatgttcc tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac
     4141 caggtaggaa aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag
     4201 tcgttggtga aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac
     4261 tggtcgctcc cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt
     4321 gtaagctttg cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga
     4381 acaatgaaat actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat
     4441 ccacctttct aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc
     4501 aaccgctttt tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca
     4561 aagagaagaa ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag
     4621 ggactttatg gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc
     4681 agggaacgct gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa
     4741 aactttttaa aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta
     4801 cccctttctg tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact
     4861 gtagtcaacg attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa
     4921 gaagcagtaa atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg
     4981 ccagctgaaa aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac
     5041 gaaaaaacac ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag
     5101 gacaatctgt caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat
     5161 tcatcgtcaa tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat
     5221 tgaaatttgc ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat
     5281 ctggtataat caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg
     5341 cgattttttt gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac
     5401 tgatatttga gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg
     5461 tcaacccttg ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag
     5521 gtgcacgatg attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca
     5581 ctgttcggat actgtcacca ataacattag cagggactcg caacatgatg gcttggactt
     5641 ctccgtcgat cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg
     5701 aactgtcttc acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt
     5761 ccttgaaaat cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct
     5821 gctcggcaaa tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc
     5881 ctggtacccg gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga
     5941 taagtgggac gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat
     6001 ggtcttggaa ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac
     6061 ctttttggat ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg
     6121 ccaactgata gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa
     6181 cttcttgctg actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag
     6241 gatgatccat ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt
     6301 caaccagcat ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt
     6361 gatctgatgg ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt
     6421 ggtcacagtc ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc
     6481 gaacttgctc tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt
     6541 caatataacg aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa
     6601 gcggtgtata aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat
     6661 ctgctaaata ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc
     6721 ccagcgcact gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt
     6781 agttcttctc actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca
     6841 taaagtttct cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt
     6901 cttcctgatg gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa
     6961 taccgcttac catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga
     7021 catcttcgaa attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag
     7081 gtggcatact taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat
     7141 aaacagcagc gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct
     7201 ctgtttgtgt ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga
     7261 ttgctggatg atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt
     7321 ctataccaaa tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct
     7381 taaaattcat cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta
     7441 agaaaaagaa cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca
     7501 aaaacacagc tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct
     7561 cctgcttttt agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa
     7621 aatcatttgg atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca
     7681 ctggtaaatg caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta
     7741 attttgtcaa aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca
     7801 tcgcgttttg accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta
     7861 attcccgctg gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt
     7921 gcagtcggcc tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca
     7981 aagcatcgaa tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac
     8041 cagcgcgttc gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg
     8101 ctgccatcaa tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg
     8161 ttgttttccc tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga
     8221 tatttagttc atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga
     8281 tgatcgcatt ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta
     8341 atgcttcggt aattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt
     8401 ctaattcttc aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc
     8461 gttcaccaaa tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa
     8521 cttcttctgt tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt
     8581 gactccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     8641 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     8701 cctagtccag catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt
     8761 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     8821 ccttttggca taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg
     8881 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     8941 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     9001 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     9061 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     9121 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     9181 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     9241 agataatgcg ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     9301 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     9361 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     9421 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     9481 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     9541 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     9601 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     9661 agacacggat aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     9721 gcaaacttag actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     9781 tcagcaaatc agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg
     9841 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     9901 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     9961 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
    10021 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
    10081 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
    10141 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
    10201 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
    10261 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
    10321 actcggcttt taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
    10381 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
    10441 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
    10501 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
    10561 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
    10621 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
    10681 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
    10741 tttctttttc aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt
    10801 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
    10861 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
    10921 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
    10981 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
    11041 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
    11101 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
    11161 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
    11221 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
    11281 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
    11341 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
    11401 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
    11461 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
    11521 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
    11581 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
    11641 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
    11701 atcggtctac tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg
    11761 tttcttgtat atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt
    11821 gcatcaaatc aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt
    11881 atttttctaa ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt
    11941 ctttttctgc tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt
    12001 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
    12061 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
    12121 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
    12181 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
    12241 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca
    12301 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
    12361 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
    12421 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
    12481 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
    12541 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat
    12601 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
    12661 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
    12721 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
    12781 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
    12841 ctcctaatac ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa
    12901 ccactgctgt gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac
    12961 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
    13021 aatgcatttt ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca
    13081 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
    13141 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
    13201 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
    13261 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
    13321 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
    13381 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
    13441 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
    13501 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
    13561 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
    13621 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
    13681 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
    13741 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
    13801 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
    13861 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
    13921 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
    13981 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
    14041 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
    14101 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
    14161 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
    14221 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
    14281 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
    14341 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt
    14401 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa
    14461 tttctttcca ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
    14521 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
    14581 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
    14641 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
    14701 tgcttctcaa acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca
    14761 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
    14821 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
    14881 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
    14941 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
    15001 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    15061 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    15121 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    15181 gtgctgcata tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat
    15241 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    15301 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    15361 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    15421 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    15481 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    15541 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    15601 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    15661 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    15721 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    15781 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    15841 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    15901 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    15961 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    16021 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    16081 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    16141 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    16201 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    16261 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    16321 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    16381 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    16441 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    16501 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    16561 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    16621 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    16681 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    16741 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    16801 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    16861 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    16921 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    16981 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    17041 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    17101 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    17161 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    17221 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    17281 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    17341 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    17401 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    17461 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    17521 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    17581 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    17641 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    17701 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    17761 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    17821 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    17881 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    17941 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    18001 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    18061 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    18121 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    18181 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    18241 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    18301 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    18361 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    18421 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    18481 gatttgtccg gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    18541 agagccagac tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    18601 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    18661 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    18721 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    18781 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    18841 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    18901 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    18961 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    19021 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    19081 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    19141 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    19201 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    19261 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    19321 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    19381 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    19441 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    19501 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    19561 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    19621 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    19681 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    19741 taataaaaaa ggacattaca cagaataaat cgttttataa caaaggt
//
LOCUS       DANJJH010000051        19768 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000051 DANJJH010000000
VERSION     DANJJH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            89..283
                     /locus_tag="PWS53_001693"
     CDS             89..283
                     /locus_tag="PWS53_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871920.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            552..851
                     /locus_tag="PWS53_001694"
     CDS             552..851
                     /locus_tag="PWS53_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871921.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(948..1382)
                     /locus_tag="PWS53_001695"
     CDS             complement(948..1382)
                     /locus_tag="PWS53_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871922.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1614..2324)
                     /locus_tag="PWS53_001696"
     CDS             complement(1614..2324)
                     /locus_tag="PWS53_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0871923.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2314..3174)
                     /locus_tag="PWS53_001697"
     CDS             complement(2314..3174)
                     /locus_tag="PWS53_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL0871924.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3179..3817)
                     /locus_tag="PWS53_001698"
     CDS             complement(3179..3817)
                     /locus_tag="PWS53_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL0871925.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3832..4131)
                     /locus_tag="PWS53_001699"
     CDS             complement(3832..4131)
                     /locus_tag="PWS53_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0871926.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4174..5658)
                     /locus_tag="PWS53_001700"
     CDS             complement(4174..5658)
                     /locus_tag="PWS53_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0871927.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5678..6139)
                     /locus_tag="PWS53_001701"
     CDS             complement(5678..6139)
                     /locus_tag="PWS53_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0871928.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="PWS53_001702"
     CDS             complement(6157..7224)
                     /gene="ulaG"
                     /locus_tag="PWS53_001702"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL0871929.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7442..8212
                     /locus_tag="PWS53_001703"
     CDS             7442..8212
                     /locus_tag="PWS53_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0871930.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8485..9312)
                     /locus_tag="PWS53_001704"
     CDS             complement(8485..9312)
                     /locus_tag="PWS53_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0871931.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9880..11115
                     /locus_tag="PWS53_001705"
     CDS             9880..11115
                     /locus_tag="PWS53_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0871932.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12114..12374)
                     /locus_tag="PWS53_001706"
     CDS             complement(12114..12374)
                     /locus_tag="PWS53_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0871933.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12401..13702)
                     /locus_tag="PWS53_001707"
     CDS             complement(12401..13702)
                     /locus_tag="PWS53_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0871934.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(13994..14452)
                     /locus_tag="PWS53_001708"
     CDS             complement(13994..14452)
                     /locus_tag="PWS53_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0871935.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14465..15796)
                     /locus_tag="PWS53_001709"
     CDS             complement(14465..15796)
                     /locus_tag="PWS53_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL0871936.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15807..17249)
                     /locus_tag="PWS53_001710"
     CDS             complement(15807..17249)
                     /locus_tag="PWS53_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0871937.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17236..18000)
                     /locus_tag="PWS53_001711"
     CDS             complement(17236..18000)
                     /locus_tag="PWS53_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL0871938.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18104..18979)
                     /locus_tag="PWS53_001712"
     CDS             complement(18104..18979)
                     /locus_tag="PWS53_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0871939.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19234..19318)
                     /locus_tag="PWS53_001713"
     tRNA            complement(19234..19318)
                     /locus_tag="PWS53_001713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19282..19284),aa:Leu,seq:caa)
     gene            complement(19336..19407)
                     /locus_tag="PWS53_001714"
     tRNA            complement(19336..19407)
                     /locus_tag="PWS53_001714"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19373..19375),aa:Gln,seq:ttg)
     gene            complement(19417..19489)
                     /locus_tag="PWS53_001715"
     tRNA            complement(19417..19489)
                     /locus_tag="PWS53_001715"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19454..19456),aa:His,seq:gtg)
     gene            complement(19551..19635)
                     /locus_tag="PWS53_001716"
     tRNA            complement(19551..19635)
                     /locus_tag="PWS53_001716"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19599..19601),aa:Leu,seq:caa)
     gene            complement(19656..19726)
                     /locus_tag="PWS53_001717"
     tRNA            complement(19656..19726)
                     /locus_tag="PWS53_001717"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19692..19694),aa:Cys,seq:gca)
BASE COUNT         6275 a         4089 c         3076 g         6328 t
ORIGIN      
        1 tatttatcat aaagatatag atacataatc attttcactt acatatattt gaatattaac
       61 taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa caaaaggcgt
      121 caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg gttcatggaa
      181 agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc tagaaaaaga
      241 actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat gtatagaaag
      301 gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta gaagaagaag
      361 acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt atttcaatat
      421 gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata tacttgtcat
      481 tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc agtatagaaa
      541 ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag caatttccac
      601 aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag tatttggagc
      661 ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata tacgagatga
      721 aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa aatcatgtgt
      781 tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag gtgagtaaaa
      841 aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat cctcttgttt
      901 ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta tttgtcatta
      961 ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc catttccttt
     1021 acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc atctgtcaat
     1081 aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt caaaagcact
     1141 ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat aaatgcgatc
     1201 gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc attacgcttt
     1261 atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt tgtgtaattc
     1321 gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga atcaatattc
     1381 atttttttct tcctttctta tacaattctc cctctaataa tacagttctt tgactattta
     1441 atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt cctctttcct
     1501 tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt cacaacctct
     1561 gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca tctctattgc
     1621 ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa atattgcggg
     1681 atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca aatttcatct
     1741 aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa cggtccatgt
     1801 ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt aaacgtctcg
     1861 acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt cgttaattgg
     1921 cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata agctggcaaa
     1981 tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt aatagcagtt
     2041 atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga tggttttaag
     2101 ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc tgcttgcatg
     2161 cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg gtccacttca
     2221 ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag atttgcttca
     2281 aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc cagcctcctt
     2341 caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt cttggctttt
     2401 ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca aatgtcttaa
     2461 taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact ggcctgaata
     2521 ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat cgatcccttt
     2581 ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta ggtctggata
     2641 tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg aaatggagtt
     2701 cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag aagcttcctt
     2761 tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt tctcttcata
     2821 ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg ccaaatggat
     2881 tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag aaccaaacgg
     2941 aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc tgaacatctc
     3001 ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc gctcatccgt
     3061 ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg aaaaacgttc
     3121 tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag ccatttcctt
     3181 acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc ggatcatttg
     3241 ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca aataagtgta
     3301 atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca atcagttttt
     3361 tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca tcacggcttt
     3421 gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt tcaaaagtcc
     3481 aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc tccatagttg
     3541 gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca acattactcg
     3601 ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta tctgggaaca
     3661 tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt gtgcccactt
     3721 caataatatc tactacatca ccaactttca taacatctcc taatgcatgt tctaagtcat
     3781 tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc tttacttgtt
     3841 tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg acatcacatt
     3901 tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat tcaaaaaatt
     3961 gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg aaatggcaca
     4021 atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca cacttctcat
     4081 catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca tcttaatccc
     4141 tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt ctgcatatgt
     4201 gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt gtaactgcgg
     4261 aatagccaat aaaccaatga caactacggc aattccagca taacctagga ctttcatgat
     4321 tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac caccgtatgt
     4381 tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga ttcctgaaag
     4441 aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa ctgctataac
     4501 ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg atttcaataa
     4561 aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc caatcgttac
     4621 cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa cagctcctgg
     4681 taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga atgtccgaac
     4741 acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca taatgtagaa
     4801 taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt tgcctagtac
     4861 aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca tattctcatt
     4921 gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt gcttgattga
     4981 tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa atataaagat
     5041 tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt cctgcgtgat
     5101 tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc ccataaaaat
     5161 caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa aagcggtagc
     5221 tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg tatatttctg
     5281 aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca tcacatctcc
     5341 aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc caaaataagg
     5401 atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga ttggccgaaa
     5461 attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg ttgcctttaa
     5521 gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta ataacacgat
     5581 aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg tccaaatgct
     5641 taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct gccatcacaa
     5701 cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca tctgtcatta
     5761 ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt gctgcgagtg
     5821 taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa cggattggct
     5881 gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt tctgaagaat
     5941 gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct acacaatgga
     6001 tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca agacagcttt
     6061 cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc aattgatggt
     6121 caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg gaaacggtgt
     6181 atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact caagatcatc
     6241 tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt taaatccata
     6301 cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat cagccataaa
     6361 attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg actccgccat
     6421 gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt caccataggc
     6481 ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat tggagtaatg
     6541 tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt ttactgcaat
     6601 ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt cacaagtgac
     6661 caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga ttttgactgc
     6721 atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt gccacgtgcg
     6781 aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa ctgctgcggc
     6841 tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt ctacattttt
     6901 tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca tattttgaac
     6961 gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat gactacgttt
     7021 tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt tcagccaaat
     7081 tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt ctgtttcgat
     7141 ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact tttcttttgt
     7201 tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt ccaacttaca
     7261 agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg tttgtttttt
     7321 atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag atatgaatgt
     7381 ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg aggcaaaagt
     7441 catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg aactgcttca
     7501 gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat ctattagtac
     7561 gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca attacggtta
     7621 ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg aaaggattaa
     7681 acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc tgtttatcaa
     7741 ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt tgacagtcat
     7801 tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt atattttaac
     7861 tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat taaatacgat
     7921 ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg tagtacatgg
     7981 ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca acaagacaat
     8041 caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa agttcaagat
     8101 tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg aaaaagtatt
     8161 agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt aagaaccagg
     8221 acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg ataaatgaaa
     8281 aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa tcgataccat
     8341 cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat caaacaatat
     8401 cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg gtattcaaat
     8461 cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct ttaccgcgta
     8521 atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta aataatcatc
     8581 ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca atgcataagt
     8641 cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg ctttatcata
     8701 atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga ccatcagatt
     8761 atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt tcgcaaaatc
     8821 taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta agtgatggat
     8881 atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg catctggttt
     8941 tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta aaagatcaga
     9001 attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt ggtattttga
     9061 tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt cttctgtttc
     9121 tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta agatattgat
     9181 gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac aaatgccttc
     9241 tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta atgctccatc
     9301 tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta tatcatacac
     9361 aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt aattttaaaa
     9421 taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt gttatctgaa
     9481 taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa agtctaagaa
     9541 tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc acacctatca
     9601 attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga cacaacttct
     9661 cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt tttttgaaaa
     9721 ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca gcgagagaca
     9781 gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt atactagtaa
     9841 gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat tctttttgct
     9901 cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa agctatgaaa
     9961 gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt gatcacagaa
    10021 agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga agaacaaatc
    10081 ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat ggttgcctct
    10141 cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat tgtgacaggt
    10201 tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt atatgttaaa
    10261 ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc ccacgagtta
    10321 cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg tatagtaaat
    10381 acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct tcctttacca
    10441 aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc taaactattt
    10501 ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt tgctaagctc
    10561 agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca agaaagaaaa
    10621 cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg caaaattttg
    10681 ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta tctgcttgat
    10741 gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag tcatcttttg
    10801 catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca aactgcctgt
    10861 gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg taatattgac
    10921 tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga aagaaaactg
    10981 cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg cacgctgcaa
    11041 gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac cttcctgagc
    11101 aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca tcagtaattt
    11161 cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat tgaaaagtta
    11221 gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata aaagatctga
    11281 cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat tctttgacac
    11341 ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat aattaggcaa
    11401 atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag agatttgtgc
    11461 cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta tctttgataa
    11521 tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact cctatataaa
    11581 ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt cgtcagcaac
    11641 gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa ttatttctat
    11701 acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca tacaccatac
    11761 tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga tcactttggt
    11821 agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc ctccttcttt
    11881 ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg acaaaagcct
    11941 tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc ggtaataagt
    12001 tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat tttcgtaaat
    12061 ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa catttaaagc
    12121 ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa aattccacaa
    12181 agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat ctttggaaga
    12241 atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat atgatatgta
    12301 gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac aaaaaataaa
    12361 atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc tggacttcgt
    12421 tttctccacg actttgttca tctgacagca tctttttatc ataagctttt acaaatggat
    12481 aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca cgccagtcat
    12541 ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc aaaacagtaa
    12601 atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa actaaaggaa
    12661 ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca aaaattatgg
    12721 gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct ttcaagaatt
    12781 ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct gcaccaccaa
    12841 ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg gtggcagctg
    12901 taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt ggacgaacaa
    12961 cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg atcaaaaaca
    13021 ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc tcgaataacg
    13081 ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa gtaattgtta
    13141 aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt ggcacttgtt
    13201 ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc actgcaaaaa
    13261 tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata ggtaagaccc
    13321 agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac ataaagaacg
    13381 ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta gctagatccg
    13441 aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta aagggtaaaa
    13501 caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt aaattgataa
    13561 agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc ccattacgaa
    13621 ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt ttctcttcga
    13681 cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg aaccattcat
    13741 tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt ttatttcaat
    13801 atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt ttctaaatca
    13861 taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat ctagtgattg
    13921 aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc tatttaactt
    13981 ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct tggttttgtt
    14041 ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta tctttttgtg
    14101 cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt acctcctggt
    14161 tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct aaacccatat
    14221 ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg gtaggaaata
    14281 tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc gttatagcgt
    14341 atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt aatggaataa
    14401 tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc actttttatc
    14461 acctctactt taatgctggc caatattttt tgtttacttt gatcatttca tctagtattt
    14521 tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt aaagcttttt
    14581 gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact tgtgcctcca
    14641 tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc acaccagttg
    14701 caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga atcgcccctt
    14761 gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa atagctatat
    14821 cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc acaataccgc
    14881 ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg accatattag
    14941 cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag taatactcta
    15001 agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc atttgaaagg
    15061 ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct tttgttatta
    15121 gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa atcgatgtat
    15181 accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga tcataaccgt
    15241 aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta atcattttta
    15301 cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt gtataattca
    15361 aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat aaacctttga
    15421 ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct actaatccat
    15481 gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca cgaatttgtg
    15541 agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca cgaatgcgaa
    15601 tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata tccatatttc
    15661 tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt tcattgttca
    15721 aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata gtgataatct
    15781 gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa ttctgccata
    15841 acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg tttaatgatc
    15901 ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc cactacctct
    15961 acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc tccaaacgct
    16021 tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa ctctgtttct
    16081 tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt ctgaatgttt
    16141 ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa aattccgatt
    16201 ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc aacaatacat
    16261 gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc tgctaaaccg
    16321 gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact aaattcaaca
    16381 ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat tcctttgacc
    16441 atctctttat tttctttata agctgcttta tagatagcta gacatatagc tggtatacca
    16501 aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc tccgttgata
    16561 aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct tcctaaatcg
    16621 taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa acgattcaaa
    16681 aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc aaaattatca
    16741 atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat tgtttgtaga
    16801 atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac tactggtaat
    16861 ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat aattccacca
    16921 aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa ggtagcaatc
    16981 gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa accgattgca
    17041 actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc tgcaccaatt
    17101 actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc taatggcatc
    17161 gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt aacattcatt
    17221 tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag gatcattttt
    17281 cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac gaggatacac
    17341 aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca tgacttcgat
    17401 cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca cactcgtatt
    17461 gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct caaaataatc
    17521 gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat catatttttt
    17581 tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat gatgactgtc
    17641 taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat tccattcctg
    17701 atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg atctgaactc
    17761 tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg tcaatgttaa
    17821 atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat gctcgaaacc
    17881 cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag catccataat
    17941 cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca cttttcccat
    18001 ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata gtccatttac
    18061 tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag attcttgcta
    18121 gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat tgctgttcga
    18181 tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat gtttcagtaa
    18241 aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta accaggaatt
    18301 tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg tttgaatagg
    18361 atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag ctctttaaac
    18421 tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct acttcatttg
    18481 accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact gctatatcat
    18541 attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca tctctatctt
    18601 ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag atatttgaat
    18661 ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca aaatccataa
    18721 attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct ttagaatcta
    18781 atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta gcagaaaagc
    18841 caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat acagcattat
    18901 gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat aaaacaaaac
    18961 aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag acaaaaaacg
    19021 acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc ctacataatt
    19081 tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac tcgttttatg
    19141 ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa aatatagcaa
    19201 ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc gaaccggcac
    19261 gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
    19321 atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc
    19381 cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga atcgaaccca
    19441 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atcataaaag
    19501 atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa taccggcggc
    19561 cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg cgtctgccaa
    19621 ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat ttgaaccggt
    19681 gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta tactattatc
    19741 aatctataaa ataaagaata aattatag
//
LOCUS       DANJJH010000052        19475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000052 DANJJH010000000
VERSION     DANJJH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(21..995)
                     /locus_tag="PWS53_001718"
     CDS             complement(21..995)
                     /locus_tag="PWS53_001718"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0871940.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="PWS53_001719"
     CDS             complement(1075..1950)
                     /locus_tag="PWS53_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL0871941.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="PWS53_001720"
     CDS             2080..3987
                     /locus_tag="PWS53_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL0871942.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="PWS53_001721"
     CDS             complement(4528..7806)
                     /locus_tag="PWS53_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL0871943.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="PWS53_001722"
     CDS             complement(8077..8622)
                     /locus_tag="PWS53_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0871944.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="PWS53_001723"
     CDS             complement(8634..9326)
                     /locus_tag="PWS53_001723"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL0871945.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="PWS53_001724"
     CDS             complement(9345..9629)
                     /locus_tag="PWS53_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871946.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="PWS53_001725"
     CDS             complement(9642..10205)
                     /locus_tag="PWS53_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0871947.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="PWS53_001726"
     CDS             10433..11095
                     /locus_tag="PWS53_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL0871948.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="PWS53_001727"
     CDS             11219..12415
                     /locus_tag="PWS53_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL0871949.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="PWS53_001728"
     CDS             complement(12516..13892)
                     /locus_tag="PWS53_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0871950.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="PWS53_001729"
     CDS             complement(13929..14795)
                     /locus_tag="PWS53_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL0871951.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="PWS53_001730"
     CDS             complement(14884..15909)
                     /locus_tag="PWS53_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0871952.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="PWS53_001731"
     CDS             complement(15927..16601)
                     /locus_tag="PWS53_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871953.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="PWS53_001732"
     CDS             complement(16785..17678)
                     /locus_tag="PWS53_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0871954.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="PWS53_001733"
     CDS             complement(17752..19449)
                     /locus_tag="PWS53_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0871955.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DANJJH010000053        19197 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000053 DANJJH010000000
VERSION     DANJJH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            99..2540
                     /locus_tag="PWS53_001734"
     CDS             99..2540
                     /locus_tag="PWS53_001734"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL0871956.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2870..3574)
                     /locus_tag="PWS53_001735"
     CDS             complement(2870..3574)
                     /locus_tag="PWS53_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871957.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3761..4006)
                     /locus_tag="PWS53_001736"
     CDS             complement(3761..4006)
                     /locus_tag="PWS53_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0871958.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4023..4874)
                     /locus_tag="PWS53_001737"
     CDS             complement(4023..4874)
                     /locus_tag="PWS53_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0871959.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5040..6044
                     /locus_tag="PWS53_001738"
     CDS             5040..6044
                     /locus_tag="PWS53_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL0871960.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6285..6641)
                     /locus_tag="PWS53_001739"
     CDS             complement(6285..6641)
                     /locus_tag="PWS53_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0871961.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7009..8196
                     /gene="tuf"
                     /locus_tag="PWS53_001740"
     CDS             7009..8196
                     /gene="tuf"
                     /locus_tag="PWS53_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0871962.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8384..10975)
                     /gene="mprF"
                     /locus_tag="PWS53_001741"
     CDS             complement(8384..10975)
                     /gene="mprF"
                     /locus_tag="PWS53_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0871963.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11001..11762)
                     /locus_tag="PWS53_001742"
     CDS             complement(11001..11762)
                     /locus_tag="PWS53_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0871964.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12040..12561
                     /locus_tag="PWS53_001743"
     CDS             12040..12561
                     /locus_tag="PWS53_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL0871965.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12620..13480
                     /locus_tag="PWS53_001744"
     CDS             12620..13480
                     /locus_tag="PWS53_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL0871966.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13665..15980
                     /locus_tag="PWS53_001745"
     CDS             13665..15980
                     /locus_tag="PWS53_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0871967.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16162..18003
                     /locus_tag="PWS53_001746"
     CDS             16162..18003
                     /locus_tag="PWS53_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0871968.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18027..19136
                     /gene="rpoD"
                     /locus_tag="PWS53_001747"
     CDS             18027..19136
                     /gene="rpoD"
                     /locus_tag="PWS53_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL0871969.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6226 a         3392 c         4028 g         5551 t
ORIGIN      
        1 aaggattgtt aaaatttaat cttagaaaaa aataactgga acaagagcta tttgttttta
       61 cagcaaagaa tggtatattt ttgtaggagg tggcttactt gaaagaagtc gttatgatag
      121 atgctgcaag aacgccgata ggaaagtatc gcggaagttt aagtccattt actgcagttg
      181 aattaggcac acttgtgact aaaggattat tagataaaac aaaactgaaa aaagataaaa
      241 tcgatcaagt aatatttgga aatgtcctgc aagctggtaa tggtcaaaat gttgccagac
      301 agattgcttt gaacagcggt ctccctgtag atgtcccagc aatgacgatc aatgaagttt
      361 gcggatctgg tatgaaagca gtgatcttag cacgccagct gatccaatta ggtgaagcag
      421 aacttgtgat agccggcgga acagagagta tgtctcaagc acctatgctg aaacagtatc
      481 agtcagaaac aaatgaatat ggtgaaccaa tttccagtat ggtcaacgac ggattgactg
      541 acgcattttc aaatgcacat atgggattaa ccgcagagaa tgttgcaaca caattttctg
      601 tgagcagaga agagcaggat cgctatgcct tgtcgtccca gttgaaagca gcacatgctg
      661 tcgaagccgg tgtattttct gaggagatca tcccagtcaa gatttctgat gaagacgtgt
      721 tatctgagga tgaagcagtt cgtggaaata gtacattgga aaaactgggc acgttacgta
      781 cagtattctc agaagaagga actgtaacag caggaaatgc ttccccgttg aatgacggtg
      841 cctctgtggt gatccttgca tccaaagaat acgcagaaaa taataatctg ccttatttag
      901 caaccatcaa agaagtagcg gaggtcggta ttgacccaag tatcatggga atcgctccaa
      961 tcaaagccat tcaaaaattg acagatagat caggaatgaa tttatcaaca atcgatcttt
     1021 ttgagatcaa tgaagcattt gcagcttctt ctatcgtagt tagccaagaa ctgcagttag
     1081 atgaagaaaa ggtcaatata tatggaggag caatcgcatt aggccatcca attggtgcaa
     1141 gcggtgcacg tatcttgact acattagctt acggattgct tcgtgaacag aagagatacg
     1201 gtattgcttc attatgtatt ggtggaggtt taggcttggc agtcttgtta gaagcgaata
     1261 tggagcaaac acataaggat gtgcaaaaaa aaaagtttta tcagcttacg ccatctgaac
     1321 ggcgcagtca attaatagaa aaaaatgtat tgacacaaga aactgcgttg atttttcagg
     1381 aacaaacact ttctgaagag ctctccgatc acatgatcga aaaccaagtg agtgaagtgg
     1441 aaatcccaat ggggatcgcg cagaattttc aaatcaatgg caagaaaaaa tggattccga
     1501 tggcaacaga ggaaccatct gtcattgcag ctgcaagtaa tggtgcaaaa atctgtggaa
     1561 atatctgcgc agaaacacct cagcggctga tgcgagggca aatcgttcta tcaggaaaat
     1621 cggagtatca agcagtgatc aatgcagtta atcatcgaaa agaagagctg attctttgtg
     1681 caaatgaaag ttatccttca atcgtgaaac gcgggggcgg tgttcaagat atctctactc
     1741 gggaattcat ggggagtttc catgcatact tatctattga ttttttagtg gatgtaaaag
     1801 atgcaatggg cgctaacatg atcaactcga ttcttgagag tgtggcaaac aagttacggg
     1861 agtggttccc tgaagaagag attctattca gtattttaag taattttgct acagaatcat
     1921 tggcttctgc ctgttgtgaa atcccattcg aacgcttagg gagaaacaaa gaaatcggtg
     1981 aacaaattgc caaaaaaatc caacaggcag gggaatatgc gaagcttgac ccttaccgtg
     2041 cagcaacgca caacaaaggg atcatgaatg gtatagaagc tgtagtggca gctacaggaa
     2101 atgatacacg tgcggttagc gcttcgatcc acgcctacgc ggcaagaaat ggattatacc
     2161 aaggattaac agactggcag atcaaagggg ataaactagt tggaaaacta acggtgcctt
     2221 tggctgttgc cacagtaggc ggagcttcca atatcctgcc aaaagcaaaa gcatctttag
     2281 ctatgttaga tattgattca gccaaggaac tagcacaagt aattgctgca gtagggttag
     2341 ctcaaaacct ggctgctttg cgtgcattag ttacagaagg aatccaaaaa ggccacatgg
     2401 gacttcaagc tcgttcatta gcgatctcaa ttggggctat tggtgaggaa atcgagcaag
     2461 ttgcgaaaaa attacgtgaa gcagaaaaga tgaaccagca gacagctata caaatattgg
     2521 aaaaaatccg ggaaaaataa aaaagcaata tctttagaag aataaagcaa gatttacagt
     2581 tattttattt actgcagatc ttgctttttt ctctatcaga aaatatgttt attattagaa
     2641 ttttttatag ttaaggttag gcatcttaag aaatcaggca actccaagat atcttcaggt
     2701 agaagagaag tcgcctaaca ttgtaggatc ataggtgatt tgttggacag gtcaagctga
     2761 tatttgagcc tgatgttaga aggaatttct ttttacttgc tgtagaaatt attatatgac
     2821 taaaaaggct gggattttaa atcccagcct tttatgacaa atagaatttt tatcgatcag
     2881 taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa agtcaatttg actttgtatt
     2941 tccgttcgag tgctttttcg ataacgaact cattttcagt agtcatagaa atagttgcta
     3001 atttacgagg ttcatcagca tttttttcat taataactac ttcatgttca ttttgttctg
     3061 tgtaaaattt aggatactca gcaacggctt ttgaaaaatc agcaaaactt aaagcggcat
     3121 tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt tggtttttcc gttttttctt
     3181 ctggcacact gactgtttct tctgtaactg gtgtcgcaac agcttgcgca gttggttcat
     3241 cgtttttcaa tcgttgatca ttccagtaag acacgatttc aataagaaga ttgtcacgga
     3301 atcgttcgaa aatcccgttt tctcctgaac gaattctctt taatacagta ttgaagttgt
     3361 catcgattgg cacgttgatc cgtgtatctt catttgtttt cccagctaat ccgatcgtta
     3421 agatttgacg gtttttggga tcaagcatga cttgtaacgg caaaccataa cgtcgcaagc
     3481 tctcttcttc tagagaacgg aataattgtc ctcttacacc atccaatgaa ggacggaatt
     3541 gatttaataa tttttgtttc tgttttttct tcatatgttc tctccttcgc tcataccctt
     3601 tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat gcaaaaaaac ttgcgtatac
     3661 gcaaattaca acagaaaaat ttgcgtattt tcccgttttt tgaaagaaaa ataaaaaatg
     3721 gaacggatta tgtatatata cataatccgt tccattcttt ctattttttc ggtatcaagg
     3781 caaaaacagg tagttcaggt gaataaatga cttcatgttc gtgccagggt ttgaccatta
     3841 gcgaatgatc gaaccgtttt ttcagttctt cgatgatttg atcatgtgtc cagtttcttg
     3901 caggaaaatg ttggattttc tctgcatcga tcaaaaagac aaaatcggaa tgaaaagtct
     3961 gggtaacttc ttccgagaga gaattcaagc gttcgattgg atccataaaa tcacctttcg
     4021 acttaaaatt gggtagtatc tgggtctttt gctttgccga ctacaccatc taactgatcg
     4081 atttgtttca tttcttcttc tgttaattca aaatcaaata attcagtatt ttctttgatt
     4141 ctgcttggtg tcactgactt aggaagagga aggaattcgt gttgcagcga ccaacgcaaa
     4201 gcaacttgtg caatcgtctt gtcatgtgct tcagcgattt gtttcatttc aggcacatcg
     4261 aagattttac cagtgcctaa tggactatat gcttcgagga tcatatcatg tttttttgca
     4321 tactcaacta cagttgtctg taattcgcct ggtgcaagga aaacttgatt gaccatcggc
     4381 ataatttttg ctgtttttgc tagtgtatct agatggtggg gtaagaaatt gctcacaccg
     4441 attgctttga ttttacctgc ttcatacagt tcttcaaatg ctcgccatgt atctgcattt
     4501 gcttgttccc aattatcacg aaaagcaaca gggtttggcc agtggatcaa aaacagatct
     4561 aaataatctg tttggagctt cttcaatgat tcttcaaaag aagacatcac aagttcatag
     4621 ctgtggtttg cattccataa ttttgttgtc aagaatatct cttctctagg aattccactt
     4681 tccttaatcg cttggccgac gctttcttca ttcttgtatc cttgggcagt atcgatatgg
     4741 cgataacctg cagccagcgc ttctttaaca gaggaaacag cgacatcccc atcaggtgtt
     4801 tgccatgtgc cgaatcctac taccgggatt tcgtaaccat ttgatagacg ataagtgctt
     4861 gttaatgaat tcatactttc agctcctttt ttattcgttc aactataagt gtagcgcatt
     4921 tttcgaaaaa aagaatccat tttgtttcta ctcttgaaat ttttgtttaa aagaaaatga
     4981 ttttgattct ggtatttttg attttttttc cgtaaactaa gacagaaggg aatgagaaaa
     5041 tggatatcta tttaaaaaaa gcaattctgc atattattga cagagaaact ggttctccag
     5101 tcttttcaca aaaagagctg gatctgacaa aagaatatat tcgtgatttt cttcaaaaga
     5161 agatccaaaa aatctcttct gctcaaacaa aaactggaca actagcagaa gaaagcatga
     5221 tcgttgaatc acttaaaatc gctcaagaaa attttgtgga agcaagtgaa gcaatcgttc
     5281 agcggtggtt cgatatctac caagaaagtg aagaagcacc tagtgcagat gtatttgttg
     5341 tcttgtatga attagataca gtgatgtact tatctttatt gaaagtcaat tatcgtgagg
     5401 cttacactca ctttgttgaa gcagacgaag cagggatcga caaccgcttg attttgaatc
     5461 gagcaattct aggggcgaaa acacaaaaag cagatgaagc attcgctgtg aatttgactg
     5521 attggaccta cgaattaatt gaaaaaaaat atgagttttc tggcaaaaaa gagctttatt
     5581 tttctggaag agtgatcgaa agtgttcctg caccttcttt agaagaaaat gtaaaagtga
     5641 tccaaaaagt agccaagcat cttggaaaaa aatttgagac agaagaattt gatatcatgg
     5701 ctgatttgaa agaagctgtc tatgatacga tagaagaaaa aggtcggttg gatcatgaga
     5761 tgattgcgga aaaagtcttc aaggaaaaca tcacggcaaa aatggctttt aaagaagaag
     5821 tacaagagca aggatttgtc ccagaagcac ctcctgtaaa agaagtacaa gaaatttctg
     5881 agaaaaaatt tggaaaacaa aaattcaagc tatcaaacgg gatcgaactg atcgtaccag
     5941 tagatgtata tcgcaaccca gatttgattg aatttgtgaa taatccggat ggtacgatat
     6001 ccgtgatgat caaaaatgtg gatgaagtat tgaatcgttt ataaaaatga ctagaatatt
     6061 aggtgttcaa gtaccagtga tcagttaaat gaattgtgtc aagagaagga tcggaaaaga
     6121 cgaatataac aaaaaaagag cgtgagacaa aagtaaagaa cacttttgtc tcacgctcta
     6181 aacacgtata aacggcggaa gcagaagcaa ctccttctta tttcttggag ttaaacgctt
     6241 ctgtcccagc ctctttttat ctaataaaat tttaagacca tttactaatt atcgattttt
     6301 tctgccgcat ttttaggcac ttcagaagcc tttatttctt cccagcttaa tacaccttta
     6361 cgaggattga ctttgagttt aagatacgca ttcatcttta atggatgttc tctttcttcg
     6421 cgaagcgtca cctgtttttt ttcaccatct ttattataag ccgcttgctt gtaggtataa
     6481 gtcgtgaatt tctctccgtt atctgcttgg gtagctgaca tttcgccatt cgtcgtgatt
     6541 tttgtataat aagcttcgcc gccgtaaaaa taatgataag ctgcaaaact tccgccgaga
     6601 aatatgccta aaacgacaag tacgccaatt aattttttca tgtacttcac ctctttcctt
     6661 attattaacg aaatagaaga tttcgtctat tgaaagttgt tggcaacagc tgaattttaa
     6721 acttttcctc attttgttct tattgcttca aaagaaagtt aaggactatc ttgaagtttt
     6781 tctcaaaaaa cacatttttt tatttataaa aggtactttt ttcgcgtata agacagacga
     6841 gtgctcttag ttgactttga ccaatattga cgtataataa gcttagttcg aatcttacaa
     6901 aaagtaagtc ggacgtattc tttttgattt agtcaaaaaa agactataat aaaaaggtga
     6961 gagctgaaat ttttcagtga tctatattat ttaggaggtt tgtaaattat ggcaaaagaa
     7021 cattatgaca gaagtaaacc acacgttaac attggtacga ttggacacgt tgaccatggg
     7081 aaaactactt taactgctgc gatcactaca gtattaggca aaaaaggcct agcaaatcct
     7141 caagactatg ctagtattga tgcagctcca gaagaacgtg aacgtgggat tacgatcaat
     7201 accgcacacg tagaatatga aacagaaaaa cgccactacg cgcatattga tgcaccagga
     7261 cacgcggact atgtgaaaaa catgattaca ggggcagcac aaatggatgg tgcaatctta
     7321 gttgtttctg caactgacgg tccgatgcct caaacacgtg aacacatttt attgtcacgc
     7381 caagttggtg taaaatacct gattgttttc ttgaacaaag ttgatttagt cgatgatgaa
     7441 gaattgatcg atttggtaga aatggaagtt cgcgagttat tgagcgaata tggtttccca
     7501 ggcgatgaca ctcctgtgat caaaggttcc gcattaaaag cattgcaagg cgatccagat
     7561 gctgaagctg ctattatgga attgatggat acagtagatg aatatatccc aacaccagaa
     7621 cgtgatacag ataaaccatt actattgcca gtggaagacg tcttctcaat tacaggtcga
     7681 ggaactgttg cctcaggtcg tattgatcgt ggtgctgttc gtgtcggtga tgaggtagag
     7741 atcgtaggga tcaaacctga aacacaaaaa gcagttgtaa caggtgtaga aatgttccgt
     7801 aaaacgttag attacgggga agctggggat aacgtaggcg tgttgttacg ggggatccaa
     7861 cgtgacgata tcgaacgtgg acaagtactt gctaaaccag gttccattac tccacataca
     7921 aaattcaaag cagaagtgta cgtgttgaca aaagaagaag gtggacgtca tactccattc
     7981 ttcaacaact atcgtccaca gttctacttc cgcacaactg atgttacagg aacaatcaca
     8041 ttgccagaag atacagaaat ggtcatgcct ggtgacaacg tgacgattga tgttgacttg
     8101 atccatccaa tcgctgttga aaacggaacg acattctcaa ttcgtgaagg cggacggaca
     8161 gtaggttcag gtatcgttac agagatcgaa gcataagaaa cgcgactcga atattttggt
     8221 aatgaaaaat gtttaaaata aaagagcggt ccaaacagga tttctgtttg gaccgctctt
     8281 tgttcttcaa ttgtataaga agagattgta gaagcatgaa ctttcgtttt acttcagtct
     8341 cttttattca tcatgagagt gttcatctat cggtatgcag tttttaatac tttcttcgta
     8401 tccatttttt caaaccactt attctacctt gtgtctgctc aactggcgca ttatcaataa
     8461 tcagcaaagc aatcataaca tatgcaatcc aactatctct agaatataga gtataacgtg
     8521 agacccagtt tgtagcgtac ttgcttttat attctcttaa tccatggaaa gaataaaaat
     8581 gagaaccaaa ctcgtaaacc aagtatgcaa tcctttcctg taaaaaactc tttctggaag
     8641 ttccgacatt tgaaagaggg gccatcccta aattgaacca ttggatatgt tcttctttca
     8701 tataggcaaa caaatgcaag aacaagaaat ccatactgcc agaaggtgct tttttagaat
     8761 catatcgcat cagatcaatc gttcctactt tattgttcgt gtaagtaggc atgatcgttg
     8821 caaaagcgac aatttcttct tgggtatttt tgacaactgc aataggcgct ctttgtagat
     8881 aatcttcaga gaaaaagcct aaagaaaacc ctttttcctt tcgtgaaccc aaccaattat
     8941 cagagatatt tttgaaagta ctcatttgct cagtagaaaa tggtggttgt aagacatcaa
     9001 atgtaaatcc ttctcgttcg atccgattca agacagcacg agtgcccttc atttttttcc
     9061 ctgaagttgt aaaactattc aggtctacat aagcttcttc ccccatctta atgaaatcgt
     9121 agccgaattc atgcaaaatc ataacaatct cttcgcttgt ttcgtagaaa actggtaaat
     9181 agcatagtcg atctgtttct tcgataaaag cttcgattgc ttctggaaaa tcttctttct
     9241 taccagaagg atcacccatt acaatacatt tattattgaa gcaagcgaat tggagaagaa
     9301 cagttggttc accgtctttt tcataggcaa acatccgctt atcttttaag aagatcaatt
     9361 gactatcgga gtttcctcca taagttgtaa ggatcttcag ggccttttct tcattaaagg
     9421 cttcgccaat ctgtttcttt tctccttgaa ggaaatgaac aaatagaacg atcatgaaac
     9481 taacagcgat gatggccaac aatcccgaaa accaaatctt ttcagaagga aataagaaaa
     9541 aggaaataaa atggtgacgc cgatgaggga aatccggcag attgtatacc ccaataacga
     9601 tgtaaagcaa ggacagtgca ccaataatga ttccatcgat cgtacgccac tcccatgaat
     9661 agatcaattg ttcccgaaat aattcgtttt tagaagcaag aatgatcaga agcagaatag
     9721 acaagaaaac caccggtgtg aaactaaaat cacttaataa aacataaagt aaagctagaa
     9781 caatcaagaa gattgtaggc aaatatgctc gtttcacacg tgctgcgatc cctctgccca
     9841 taatgattaa aagaaatccc aatagtatac taggaaattg gataataaaa tgaaatttca
     9901 atgggttcaa actatgaagc cagcgaaact ctgtaaaagc ttgcgggata gtggcagaaa
     9961 gtactagcat gattccagaa aagtataata agacaaccac gattttatgc gcaatttctg
    10021 ttgctaattg tttagggata ccagaataac gctgatcaaa agtggaaccg atatttttga
    10081 agaaaaagac gattccaatc aaaaacggaa tgatgtagta gaacaggcga tagagcaaga
    10141 tccacacaac gactatttct cttggcacac caatagcgga caacccgata atcatcatta
    10201 catcgaaact cccaatttct cctggaatca tagacacgat tcctatcact gaagcagcaa
    10261 tgaataatgg aattgtttgc cacagatcaa tccggatttc cattaaataa ccaacagaaa
    10321 ggaaactaaa caggacaccg gtccattcca acaatgaaac tagcaatagc tgtccttgag
    10381 ttttaagtga ggtaccacca aggaatcctt tctttttgaa agtagtaaat aaaaagacga
    10441 caggaaaata tagaccgccg ccgatcaacc atatccaata ttgcttcaaa aaaggagcaa
    10501 ctggcgtgaa gactacaagt aaaaaagaaa gcagactgta aatagaaagt ccagccatca
    10561 aaaacaagaa aactttcgat aatgcttgga tcacgtttct gctctctttc ttttgtccat
    10621 atagttcaga gcgaagcccc acactaacaa acccgccgaa accggcaata ttgttgatcg
    10681 tattgatcag ccagcttgtc tcaaacaggt aacgaggatt ctgcttttgt tcaagcatcc
    10741 gattcaaaat aatatcataa ccaaccatag gtaacacaga gataagacca atcaatagca
    10801 ttagtccgct tttccataca ggtatagtgg caaacgtttc tcctaattgc tggatagata
    10861 gagttttacc gatggacatc aattcaccga caataatgac aatgacagaa atcaaaaaga
    10921 ttgttttgaa cagtcctagg tggtttttca gccattgtat ggtatttttt aacaaataaa
    10981 tcaccttcta ttcctaatag ttatgtttca gaaagaaatt gaagaacttc tcgattgaag
    11041 cgttcaggat cttttctagc taattcatgt ccttgttctt tcacaagcac aaaggaagct
    11101 tttggtatag ttttagctat atacaaagaa tgttttagct tgattacatc tttttttccc
    11161 acgatgatca aagtggggga gttgattttt tttaaatcat tttctgtaag cccgatgtca
    11221 tgcagcaaaa gtttgataac taaaagattt ttgcgaagac ttgggcgaaa taaagagagt
    11281 aaccataccc atgcataatg aatattactg atgactctgg cagaaaaacg aacgccttta
    11341 acaagcgtgt ttccagaatt taaaatcaat cgatgaactt ttttagggaa gctgctggca
    11401 aataccagtg ctagatttgc tccatcacta aatccaagaa aatctgcctg atcgattttt
    11461 tccagcaaca tgatcgtatt cagatcctcg gccattaact gaaaattcaa catagatgct
    11521 tcgtttgtcg aacgaccatg tcctcggcta tctactagat acacggtata gtagcgttcg
    11581 agtacaggga cctgttctga aaaaaagcgg ccgctgccgt cattgccatg cagtaaaaag
    11641 agagggaagc cttgaccgct cttttcatag taaatttcgc tcccatctgg catttttgca
    11701 tatttctttt cttttttcac ataatcacct actgtgtaaa gtataacgat tatatcagtc
    11761 attgggcaat tgtttttact gaaaaacaac gatcattttc gtaagatgtc aaaaaataca
    11821 ggaaaattca ttttttctta aggcgtacac agaaatcaag gaagaaatag ccaagaagat
    11881 aaagaaatag taagataaaa aaagatgtca aaataactag gtgatgaaat gaagcgaaaa
    11941 cgattatcta ttatccgtat tcttttttac atgatcattc tgacggttat tggtgcggtg
    12001 ttgtttttat gaggaagaac ttatcgttcg gtaaaacgag tggaaagcta tcaaatagaa
    12061 ataacaacag ctgcaaaaaa ttatcatatg gaaaactacg agaatttgat caaggcgata
    12121 atattgaccg aatcaaaagg aaaagggaca gatctcatgc agagctcgga aagtctgcat
    12181 ggagaagctg agatcattga taatccagct gacagtattg atcaaggtgt caaatatttg
    12241 gcggaaatga tagtagaagc aaagaaacaa ggatgcgatt tatggacagc tgtccaagca
    12301 tataattttg ggaaagatta tattgcctat gtaaaaaatc atggcggaga aaacacagta
    12361 gaattagcag aagcgtattc taaagatgtg ctgagcccac ttttaggaaa tgaggaaggg
    12421 aaacaatacc gatattggcg gatccagtcc ttgctttaca atggcggcta tctctatcat
    12481 aatggaggta acatgtttta tcctgatatt gtcaaaatga atgaaaaaat aatagagacc
    12541 tatgaaaaag tctttaagta aaatagcagg aaatgttcaa tcatgaataa aattaaatga
    12601 aagaaggagt gcgcaaatag tggaaaaaag gcaaaaaatg gaaaatcaag aagtggggta
    12661 cacaaccaaa gactggatgt tacggtttgt gaagggaatg tttatcggtt caggatttat
    12721 cttgccagga gtcagcggtg gtgcattagc agctgtcttt ggcatgtatg agcggctgat
    12781 ttctttttta gcccacctta caaaaaattt caaagaaaat gttttgtttt ttctgcctgt
    12841 tggtctaggc ggagtgacag gcatctttct actttctttt ttagtcagtt ttttattagg
    12901 tgcgtatgaa acaacgatat tatggttttt tgtcggatgt atcgctggaa ctgttcctgc
    12961 gttatggaaa gaagcaggga aaaaaggaag agatacttct gatgtgatca tcatggttgt
    13021 tacttttgtt ctggcttatt tgtttttatt atacggcgct caattatttg acgggcaagt
    13081 cgatcagaat tttggaacat ggctgattgc cggcggattg attggtttgg gtatgatcgt
    13141 cccaggattg agcccttcta attttttggt gtatatggga ttgtataaag cgatggctga
    13201 cggatttaaa gaagtacgac ttgaagtgat tgtgccgatc ggaattggtg ggattctttg
    13261 tgttcttgga ttgtcaaaaa tcatggatta cattttctca aaggcatata gcaagctatt
    13321 ccatttcatt ttaggtgtag tttttgcttc aacgattatg atcattccta ccgattacgc
    13381 gggattggga gttgctgggg tactagcttg tcctgttctt tttatagccg gtacagcact
    13441 aggttggtgg atgagccgtt tggaggaaca atataagtaa agatagattg ataataaaaa
    13501 cagcacacgg gtgattcaaa caaaaaggtt ggcatctttt ttgtaaaatc attcgtgtgt
    13561 ttttctataa attttataaa aacaagttgt ttttaatctt ggaacacgct agacttaaac
    13621 acgtgattat ttttgaaagt tggcggaaaa tggagaaaaa gaaaatgaat ggacttacac
    13681 agcaagaggt agttcagaaa atggcttctg gccagcaaaa tgactaccag gaagatgcat
    13741 caaaaagtac aaaacaaata ttcagcgata atatattgac gttattcaat tttctgaact
    13801 ttggtattgg gatttgtctg ttatttgtag gtgcgtattc caacatggcg tatttagcaa
    13861 ttattgtcgt caatatcgtt attggaattt atcaagaaat acatgcaaga aatcttgtac
    13921 ggaaattatc gattgtttcc caatcagaag tactagtgat ccgggacggt aaagaacaat
    13981 cgatttctac taaagagctg gtattaggag atttggtaaa aatcggagca ggagaacaga
    14041 taccagccga tttacaggtt atatctggac gggcagaagc aaatgaagct cttttgacag
    14101 gcgaatctga tttgattgtc aaagaaactg gagctgaatt gctttctgga agctttttga
    14161 caagcggttc tgtcatggct gaagttatcc gagtaggtgc agataattat gctgtccgct
    14221 tgactagcga ggcaaagact cacaaagcta ttcgttctga acttgtagat tcgattcgca
    14281 aagtatccaa attcactagt ttcgtgatta ttcctttggg gatcatacta tttttggaag
    14341 cactgttcat ccgcgatgct tctatcaaaa tttctgttat tgcatctgca gcagctttgt
    14401 taggcatgct tccaaagggt ttggtattgc taataagtat tgcattaacg acaggggtaa
    14461 ccaaactggc aaaaaaacga atccttgttc aagacatgta ttcaattgaa acgctggccc
    14521 atgtcgatac attatgcttg gataaaacag ggaccattac tgaagggaaa atgagtatcc
    14581 agcgggtaga agcactacat ccgctgtacg agaaagccat cccagaaatt atggggacgt
    14641 atctggcaga aagcagtgat aacaacatca ctatgcgggc tatgagagat tattttgagc
    14701 tttctaatcg gtatgatgtg acaaacaaag tcgcattttc ttctgatcgc aaatggggcg
    14761 cgatggaatt tccagaactg ggagtcgtgt acgtaggagc gcctgaacgt ttggtggaag
    14821 aagatcggct tccggacagt ctaaaaacag cccaagcgaa tggctatcgc gtacttatgt
    14881 tggggatcgc tccaaatcag caattgaacg aagaaaaacc agcatttgtt gagccggttg
    14941 ctctgtttga aatcgatgat ccaatccgaa aaaacgctca agaaacacta gcttatcttc
    15001 atgaggaagg tgtggatctg aaagtgattt ctggagacaa tccttttact gtctccaata
    15061 tcgctagacg tgcaggatta ccgaattatg atgcgtatgt cgatctttct caaattactg
    15121 aagaaatgga agtgcgagaa gctgctcatc gctatacggt ttttggacga gtgagtccgc
    15181 agcaaaagaa attgctggta aatgaattga aagaaagcgg cagaacggtt gcaatgacag
    15241 gcgacggtgt gaatgatgtt ttggcgttaa gagaagcaga ttgcagcatt gctatggcag
    15301 aaggtgatgg agcaacacgt cagattgcga atctggtatt gttggactct gattttacta
    15361 cattgccaga agtcctgttt gaaggacgcc gagtggtcaa caatgtgacg aaagtctcag
    15421 gcatcttttt tatcaaaacg atttattcat ttattttatc gattatatgt gccgttacag
    15481 caattggatt tccgtttatc cctatccaaa tcacgctgat tgatttagcg attgaagggt
    15541 atccttcctt ctttttatct tttgaacaag ataaaagaaa aatcacttac cgttatttgc
    15601 ctacagcact tattaatgct ttaccaaatg ccttgcttgt agtagtgaat atcattgcgg
    15661 tgtatctact tggaaaaaca ttcgcatggg gaaatgctga aactactact ttgatgtatt
    15721 acttgctgat tgggatcagt tgcatggcag ttgtaaaagc ttgttatccc ttcaacccat
    15781 tgcgcatctt cctagcagta acgacgatcg tagggatata tgtagctgca atgctgttcc
    15841 atggaatttt ggaagtaaac ttagggttgg gagagagcgt aggatacttc tttgtattca
    15901 tgctcgtaaa tattgttctt cgagtattgt actggaaggc tgatattcaa aatcagttac
    15961 tgaaaagagt agcaaattga tgcttaatga gatagaaatg tgttatactt gaaaaagcaa
    16021 tgaaatatga tacaatcaaa catgtataat tgtgtccttt aaaatcgcac tgatttcagt
    16081 tagtgcgatt ttttaccggt tattctcctg aaatatcttc tgttaaaccc aaaagatatc
    16141 aatagagcag ggggtgaaaa aatggctcaa cggattcctc aagaggttat cgaaacagta
    16201 cgcagtcaaa cgaatatcgt tgaggtgatt ggacaatatg tccagttgaa aaagtctggt
    16261 aaaaattact tggggctgtg tccttttcat gaagaacgta caccgtcttt ttctgttgca
    16321 gaagacaagc agatttttca ttgctttggt tgtggtaaag gcggaaacgt gtttactttt
    16381 cttcaagaac tagaaggtct aagttttcca gaagcagtgg taaaagtggc agacttcgaa
    16441 cagataccgt tagaagatca atggcgtcag cgtactatag ctgtccaaaa tcctgaatct
    16501 gctacgggga aactgattgc tgctcatgaa aaagcagcgg aaatctatca tcacatgcta
    16561 ttgcatacaa aagctggaca gccagcttta gaatatttgc aaagtcgtgg gttgtcttta
    16621 gaattaattg aagaattcaa cataggtttt gcacctaatg agcgttcttt tcttcagcaa
    16681 gtattcaaga atgaatcaat ttcaaatgaa ttgttagaac gctctggatt gtttgtcacg
    16741 catgatgatg gccgtatgtc tgatcgtttt tatcaaagag tgatgtttcc acttcgtaat
    16801 tctcaaggcc agacgatcgg tttttcagga cgctggcttg cacctgaaaa agggcaggaa
    16861 aaagatcagc ccaaatactt gaatagtcca gaaacagaac tgttcaataa aagacaagtt
    16921 ttatttaatc ttgataaagc aagaagcgat atccggaaaa acggagaaat tttgttgttc
    16981 gaagggttta tggatgtgat tgctgcatgg caggctggta tcaaaactgg attagcttct
    17041 atggggacaa gtctaacagc tcaacagatc caacaaatgg aaaagctgac gaaagaattg
    17101 gtcttttgct atgacggaga caacgcaggt tttgaagcaa ccaatcgagg gattgaattg
    17161 cttcggcaaa acagtcgatt agcactttct gtcgtagtcg ttccagaaaa gcttgatcct
    17221 gatgaatatc tccgtaagta tggagaagca tctttccaag aattagtggc tcatgggcgc
    17281 gaaacagtct ttacttttaa atcacgttat ttaaaacaag ggaaaaattt agataatgaa
    17341 aaagacaaga tcgaatacct cgaagaatta ctagttgaac ttagccgagt tgtttcacca
    17401 atcgaacaag atatgtatct atctcaactg tcaacagagt ttcagatcgg cagagagacg
    17461 atacaaaaac aactgcgcga tcttcgaagg acaaatcagc aaaatcgtca agaacctcag
    17521 gaaagtcaac acactcaggt tacgagacaa gaaactgcac ggaaaaagag acctttgacg
    17581 caagtagaaa aggcagagca gattctatta tatcgtgcat ttaaagaagt ttctgtccgt
    17641 aatattttaa aagaacgaga atttcagttt attcatgatg catatgcaga agtttatttt
    17701 ttattcgatg catatgtctc acaacattat gaattcaacc ttgcagagtt tttagacttt
    17761 ttgcaagatg aaaatttaag gcggttggtg gtggaaatcg aatatttacc ggtggcagaa
    17821 gaaagttcgc cacgagaaat ccaagacttg ctagatgtga tcagcaaatc tggcttagct
    17881 gatgaaataa cattaaaaaa acaaatgcag caagaagcca gacgaactgg aaacaaacag
    17941 cttgagcttg aattgacgat tgaaatcatc aatttaacga aacagttaaa acaagccaaa
    18001 tagctactca aggggggcct tcttttatgg caaatggaac agataaaaaa acgtatgaag
    18061 cagcagtagc agctttcatc aaagaaaaca aacccaaacg acaagttgta tatgatgatt
    18121 tatccaatca gcttgctaca ccattcactt tgaatgcaga tgaaatggac aaattgatcc
    18181 aaaagattga agacgcaggt atcagtgtgg tggatgaaaa tggtgatcca tcaattcaca
    18241 gcttgaagag tgctgagaaa aaagctgaac aagcaaaaac agaagatctt tctgcgccaa
    18301 ccggtgtaaa aatcaatgac ccggttcgta tgtatctgaa agaaattggc cgtgtctccc
    18361 ttttaactgc ggaagaagaa gtagcgttag cattgaaaat cgaagaaggc gaccaagaag
    18421 cgaaacagcg tctggcagaa gcaaaccttc gtttagtagt aagtatcgct aaacgttatg
    18481 taggacgagg catgcagttc cttgacttga tccaagaagg aaacatggga ctgatgaagg
    18541 cagttgaaaa atttgactac cgcaaaggat ttaagttttc aacctatgct acatggtgga
    18601 ttcgtcaagc catcactcga gcaatcgctg accaagcacg tacgatccgt attccagttc
    18661 atatggttga aacaattaat aaattgatac gtatccaacg tcaattgctg caagaccttg
    18721 gaagagaacc tacgccagaa gaaatcggtg cggaaatgga tcttccaaca gaaaaagtcc
    18781 gagaaatctt gaaaatcgcg caagaacctg tttcccttga aacgccaatc ggtgaagaag
    18841 atgactctca cttaggtgat ttcatagaag accaggaagc aacaagtcca gcagaacacg
    18901 cagcttatga gttactaaaa gaacaattgg aagatgtact agatacattg actgatcggg
    18961 aagagaatgt ccttcgctta cgttttggtt tagatgatgg ccggacgaga acattggaag
    19021 aagtagggaa agtatttggt gttactcggg aacgtattcg tcagatcgag gctaaagcat
    19081 taagaaaatt gcgccatcct tctcgttcaa aacaattaaa agatttctta gaataagcgt
    19141 ggactattga ccagaagaga aatcagtaaa actgtttggt ttattttaaa agaaaaa
//
LOCUS       DANJJH010000054        19130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000054 DANJJH010000000
VERSION     DANJJH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..703)
                     /locus_tag="PWS53_001748"
     CDS             complement(<1..703)
                     /locus_tag="PWS53_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0871970.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="PWS53_001749"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="PWS53_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="PWS53_001750"
     CDS             1758..2837
                     /locus_tag="PWS53_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0871971.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="PWS53_001751"
     CDS             2869..3462
                     /locus_tag="PWS53_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0871972.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="PWS53_001752"
     CDS             complement(3572..3799)
                     /locus_tag="PWS53_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0871973.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="PWS53_001753"
     CDS             complement(3902..6490)
                     /locus_tag="PWS53_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL0871974.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="PWS53_001754"
     CDS             complement(6510..7169)
                     /locus_tag="PWS53_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0871975.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="PWS53_001755"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="PWS53_001755"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL0871976.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="PWS53_001756"
     CDS             complement(7942..8808)
                     /locus_tag="PWS53_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0871977.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="PWS53_001757"
     CDS             8917..9558
                     /locus_tag="PWS53_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL0871978.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="PWS53_001758"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="PWS53_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL0871979.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="PWS53_001759"
     CDS             complement(10999..11898)
                     /locus_tag="PWS53_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0871980.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="PWS53_001760"
     CDS             complement(11900..12697)
                     /locus_tag="PWS53_001760"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL0871981.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="PWS53_001761"
     CDS             complement(12675..13379)
                     /locus_tag="PWS53_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL0871982.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="PWS53_001762"
     CDS             13510..14085
                     /locus_tag="PWS53_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL0871983.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="PWS53_001763"
     CDS             14203..14880
                     /locus_tag="PWS53_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0871984.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="PWS53_001764"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="PWS53_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL0871985.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="PWS53_001765"
     CDS             complement(16943..17941)
                     /locus_tag="PWS53_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL0871986.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="PWS53_001766"
     CDS             complement(18167..18820)
                     /locus_tag="PWS53_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL0871987.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DANJJH010000055        18206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000055 DANJJH010000000
VERSION     DANJJH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..152
                     /locus_tag="PWS53_001767"
     CDS             <1..152
                     /locus_tag="PWS53_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0871988.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="PWS53_001768"
     CDS             complement(230..763)
                     /locus_tag="PWS53_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL0871989.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWS53_001769"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="PWS53_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL0871990.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWS53_001770"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="PWS53_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL0871991.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="PWS53_001771"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="PWS53_001771"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0871992.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="PWS53_001772"
     CDS             complement(4488..5066)
                     /locus_tag="PWS53_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL0871993.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="PWS53_001773"
     CDS             5388..5858
                     /locus_tag="PWS53_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL0871994.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="PWS53_001774"
     CDS             complement(5966..7192)
                     /locus_tag="PWS53_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0871995.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="PWS53_001775"
     CDS             complement(7946..8146)
                     /locus_tag="PWS53_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0871996.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="PWS53_001776"
     CDS             complement(8280..8888)
                     /locus_tag="PWS53_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL0871997.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="PWS53_001777"
     CDS             complement(8973..9443)
                     /locus_tag="PWS53_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL0871998.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="PWS53_001778"
     CDS             complement(9491..10405)
                     /locus_tag="PWS53_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0871999.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="PWS53_001779"
     CDS             10551..11354
                     /locus_tag="PWS53_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL0872000.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="PWS53_001780"
     CDS             11532..12479
                     /locus_tag="PWS53_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL0872001.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="PWS53_001781"
     CDS             complement(12547..12840)
                     /locus_tag="PWS53_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL0872002.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="PWS53_001782"
     CDS             complement(12868..13533)
                     /locus_tag="PWS53_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL0872003.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="PWS53_001783"
     CDS             complement(13673..14305)
                     /locus_tag="PWS53_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0872004.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="PWS53_001784"
     CDS             complement(14312..15379)
                     /locus_tag="PWS53_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0872005.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="PWS53_001785"
     CDS             complement(15376..16107)
                     /locus_tag="PWS53_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL0872006.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWS53_001786"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="PWS53_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL0872007.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWS53_001787"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="PWS53_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL0872008.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5409 a         3688 c         2909 g         6200 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatc
//
LOCUS       DANJJH010000056        18090 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000056 DANJJH010000000
VERSION     DANJJH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            37..360
                     /locus_tag="PWS53_001788"
     CDS             37..360
                     /locus_tag="PWS53_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL0872009.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            542..1912
                     /locus_tag="PWS53_001789"
     CDS             542..1912
                     /locus_tag="PWS53_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL0872010.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2042..3343)
                     /locus_tag="PWS53_001790"
     CDS             complement(2042..3343)
                     /locus_tag="PWS53_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0872011.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(3697..5655)
                     /locus_tag="PWS53_001791"
     CDS             complement(3697..5655)
                     /locus_tag="PWS53_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL0872012.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5832..6098)
                     /locus_tag="PWS53_001792"
     CDS             complement(5832..6098)
                     /locus_tag="PWS53_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0872013.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6608..8734)
                     /gene="pnp"
                     /locus_tag="PWS53_001793"
     CDS             complement(6608..8734)
                     /gene="pnp"
                     /locus_tag="PWS53_001793"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL0872014.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(8922..9191)
                     /gene="rpsO"
                     /locus_tag="PWS53_001794"
     CDS             complement(8922..9191)
                     /gene="rpsO"
                     /locus_tag="PWS53_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL0872015.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9442..10005
                     /locus_tag="PWS53_001795"
     CDS             9442..10005
                     /locus_tag="PWS53_001795"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0872016.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10258..10779)
                     /locus_tag="PWS53_001796"
     CDS             complement(10258..10779)
                     /locus_tag="PWS53_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL0872017.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10782..11624)
                     /locus_tag="PWS53_001797"
     CDS             complement(10782..11624)
                     /locus_tag="PWS53_001797"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL0872018.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11617..12342)
                     /locus_tag="PWS53_001798"
     CDS             complement(11617..12342)
                     /locus_tag="PWS53_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL0872019.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12518..12814
                     /locus_tag="PWS53_001799"
     CDS             12518..12814
                     /locus_tag="PWS53_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872020.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            13382..14974
                     /locus_tag="PWS53_001800"
     CDS             13382..14974
                     /locus_tag="PWS53_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0872021.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            14987..15727
                     /locus_tag="PWS53_001801"
     CDS             14987..15727
                     /locus_tag="PWS53_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0872022.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            15895..17244
                     /locus_tag="PWS53_001802"
     CDS             15895..17244
                     /locus_tag="PWS53_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0872023.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(17372..17962)
                     /locus_tag="PWS53_001803"
     CDS             complement(17372..17962)
                     /locus_tag="PWS53_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0872024.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         5199 a         3685 c         3482 g         5724 t
ORIGIN      
        1 aataaaacat tcgttgattc gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc
       61 cgcaaccgtt acgcttcagt tggtgtcgta tcaagtttgc cagatgaatt gattgacagt
      121 atatggttta ttattgacct agatcttaag ggagtcattc cattagaaaa tatattaacc
      181 tttgatttga tcgaaaatcg tggaaaagtg acgatgcatt tttctcaagc aggaagcaat
      241 gtggaaatgg atattgattt gccgtttgcc tattcgaacc aatttcctgc tcaagtatac
      301 gcctatgatg acggtacgag agaaacgatc cttttaccaa gtgagattag acaatattaa
      361 ggtgatagat aaacgtggat gtcccttatt ttacagacca ggccaagttg ttgattcttt
      421 gactcaattc ttggcttctt tttatggagt ttcataaatt atcattgatt tctaattggt
      481 attctcagga taaacgctat atactactaa ctaaatttta aagatataag gagtttaaaa
      541 gatgcgcgat ttaacaaaag gaagccccgc taaactgatt ttgatgttca ctgttccttt
      601 actgatcgga aatgtttttc agcagttcta caatatgatc gatatgatca tcgtgggtca
      661 gacgctaggt aaaaatgcgc tggcagcggt tggtgcaact ggaagcctga catttttgat
      721 cattggcttt gcccaaggtt taacagctgg tttagccatt attaccgcac aacgttacgg
      781 tgcaaaagat tatcggggtc tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt
      841 cgtcactgtg attttaactg tcctaagtct cctgtttatt cgtccgatgc ttcaattgat
      901 gcaaactcct cctgaaatac ttgatcaagc acaaacattt atttcaatta ttttattagg
      961 gatctttgca tcagtcagtt tcaatttgct ttctaatgtc attcgcgcac ttggtgacag
     1021 ccgcacacca ctattctttc tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat
     1081 ctttattatc tattttggca tgggtgtgga aggtgcagct attgctacag taatcgcaca
     1141 agtttcttcc agcgtactgt gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt
     1201 acgcaaaaaa gatttcaaat ttgaaaaaga agagtttgct gtacacctaa atgctgcttt
     1261 gccaatggcg tttcaatctt cgatcatcgc tgttggtgct atcgtgttac aagcagcact
     1321 aaatagtcta gggacagatg tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt
     1381 tgcaaatcag ccgatgatgt catttggtat tgcaatggca actttcactg cacaaaacta
     1441 tggtgcgaaa gaatatggcc gaatcttgca aggggtacgt caaactttac tgatgagtgt
     1501 cggatttagt ctcttagcag gtgctatcgt cattttcttc ggtcatttct ttgttcaatt
     1561 gtttgttagc ccatcagaaa cacgtgtatt tgaattagca caaacctact tcaatatcaa
     1621 tgggtctctt tactggatct tagcagtcct gttcatttta cgctacacac ttcaagggct
     1681 tggacaaagc aagatcccta cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc
     1741 agctatcgtt cttacaggaa tgtggggata cccaggagca gcagcagctt ctcctttagc
     1801 ttggatcggt tctgttgccg tgttgcttta ctcttatatg cgttcgatga aacaattgaa
     1861 acacatgggt gaagaggaac atttcacatt agaacatgca gaagcaagat agccttgatt
     1921 aaaagaaatc gttgcggcta aaaatgaaaa cttttattga aaatatcttc ttcgtttacc
     1981 gaaaacaggt tttttagata acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct
     2041 tttattctgt ttttctttcc gtggactcta ctttgaaata atcgatatgt gcatgctggt
     2101 attctaatac cgtgaaacgg aatgatgcaa tctcaatgat tgttcctgct tttatttctt
     2161 tatatcggct agaagcaaag cccgcaagtg tagtaaaatt cgtttcttca aactctggaa
     2221 tctctacatg aaaataacgc tggaaatcat ctaatgttag tttgccggag atgatataag
     2281 agccatctga ctgttccgct atcaatgctt cgtccaaatc tgtctcatct cggatttccc
     2341 cgacgatttc ttctaaaata tcttcaatcg tcactaagcc gcttgtccct ccgtattcat
     2401 cttttaagat agcaatatgc ttaccctttt ttcgcatgat cgtcaataat ttttttatag
     2461 ggatcgtttc agttgttacg atcggctctt caatcaactg ttctactgtc agctggtcat
     2521 ctttataaga ttgcttgatc aaatctggca aggtgacata acctaaaaca ttgtcttttg
     2581 attctaagat aaccggatag cgagtatgtc cttgttcaat cgaagctttt atcgcttctt
     2641 tgacggtttc tgaagcttcg aatacttgca tggacgtacg gtctacttgt atatcttttg
     2701 ccaaggtatc atcaaattca aacacgtttt ccaaataata atattcttct ttggtcaact
     2761 ctcctttttc ataagaattc aatgcgacta atgtcagctc ttcttgagac aatgtctctt
     2821 ctccttcaga tacgagacgc acacctaata gcttccctag ccctgcagct gaatgattca
     2881 atacccaaat aaaaggaaac attactttat aaaaataatg aagtggttta actacgaaca
     2941 agacgacctt ctccgtattg acgatactat agctcttagg aagcagttcg ccaataacca
     3001 catcgatata cgtaactaaa ataaatccca gcgccacaga gattgtactg ctaatagccg
     3061 aactaaaagg tagtttaccg atcactggtt ctagtaaagc cgcgacagat ccttcaccga
     3121 cccacccgat gatcaaaccg gttagcgtaa ttcctaattg actagctgat aaatagttat
     3181 ctaaatggtg tgtaacatga agagctagct tggcgttctt tacattttcc ttttctagtt
     3241 gttccaatct tgatcgtctg attttcacta aagcaaattc acttgctaca aaaaatgaag
     3301 taattgcaat catcaaaaat aaaataatga aattgataac catattctcc cgcctttctc
     3361 tttttctatt gtaccctttt tctacaaaaa gattgaaaat aatgcgtatt catatttagg
     3421 aataaaaaag gagatgcggc atttttcagc cacatctcct tatcacttca aaatcattgc
     3481 gttacttgtt gctaaacagc tttttcatga cctctgataa aaacggtatt caaaagcttg
     3541 catctacggc attaagccca aacagatgaa aaacatgaga agctgttttt atctgctcgt
     3601 tcttactgct tgttgcagta cagctttttc atgacctctg ataaacggtg ttcaagtagc
     3661 tgacattcga cattagttca ggtcaaagcg ctactttcac aaccgctctt tcaaccatgt
     3721 tttccgagta tttccaatga taagcggaac agtttcatgt tttacttcgc tgtactctag
     3781 cccaaaccat gtttctggcg aggtagcgat ttttttgatt gccagttttg cctgcatgat
     3841 ttgacgttgc cattttccta gagttgtcac attggaaact acttcatcaa tcaggacaaa
     3901 ttggaaatcg ccaacttctc gccctggtgt cagagagtat ctttgtggct gttttggcaa
     3961 acggccttgt ttcatcaaat catggatgac ttggcgaaca tatacgttga tttcctgttg
     4021 gaccctgaaa cctaagaaca gattcacttg gacaataaaa tcagtgtcca tcatatctac
     4081 ttgatattct ttcgtatatg gctcatccgt cacttccaca tgaacaaacc aatagactcg
     4141 tgctctttta ggccgtttat ctaaaatcga atagatgaac tgtcggccaa ctttatcttc
     4201 agccatatca ggaaccaaga agaccacatt tgtctgatag attggtaaag aagtatcatc
     4261 acgtaattct ctaagctgcg ggatataatc tctcaaagac acatcctcgg ttgacgattc
     4321 gcgaataatg ttcccttgtt cccaaataaa catcacgact aagatgatca aaccaatcag
     4381 aacggctaca aaaccgcctc ggaagaattt gacaacactt gagataaaga aaacgacttc
     4441 tatcgcaccg aagaaaaagg taactaaata tgcaagccaa cgagaatgcc cattttggat
     4501 aagataaaag taaagcagtg ctgttgtcat caacatcgtc acagtgatcg atagaccgta
     4561 agcagcttcc atatgatgag acgttcggaa tgtgacaacc actagagaac aggcgatcca
     4621 taaaatcaga ttgactgctg ggatatacat ttgcccgata ctgttcccag gatacatgat
     4681 ctttaaccga ggcagcagtt tcaacttgat tgcttctgaa actagcgtaa acgaacctgt
     4741 aatcaatgct tgcgaagcaa tgactgcagc gactgttgca aaagcaacgc cgatcacgat
     4801 ccaactttga ggcatcattt ggaagaatgg attcaggttc ttgatttgct gtgcttcagg
     4861 gttttgataa acattcaaca accacgcagc ctgcccaaaa taattgataa ccaaacaaat
     4921 tttgacgtaa ggccagctca gacgaatatt tcgtttgccg acatgtccaa gatcggaata
     4981 aagcgcctct gcacctgtcg tagccaggaa aatatttcca agaatgaaca agcctaattt
     5041 gttatctgca ctaaaaagca gctggatagc ataataagga ttcagtgcac ggataactgt
     5101 ccagtcttga ctgaagttca ttaatcccat tacgcctaaa aaagtaaacc aagcgaacat
     5161 gattggtccg aatgcttttc cgacaatatc tgtaccaaag cgttggacca tgaataaaac
     5221 aaaaataata actaaagtga taatgacgat cacgttctgg ttattaccga accggtcaaa
     5281 aaactgcgga attcctcgca agccttcaat cgcagtcgtt accgtcacag caggggtcaa
     5341 taccccatct gctagtaagg cagctcctcc gatcattgca ggaatgatca aatatttccc
     5401 accttttcgg acaagtgtgt agagtgagaa aattccacct tcaccatggt tatctgcatt
     5461 caatgcaatc acgacatatt tgattgttgt cagtagagtc aatgtccaaa aaatcaatga
     5521 gactgatccg ataacaaaat caggattgat tcctgacaat ccgccattat cttcaacaat
     5581 cgatttcata acgtaaagcg gacttgtccc gatgtctccg tagacgactc ccatcgctac
     5641 aagcaggcca gtcattgata tctttttggt attgtgcgtt gttgccacgt ttttccctca
     5701 tttcttgtta tatggatatc ctttaggtaa gcttgaatga tagatgatat tcttatccat
     5761 gacacccgct ttcctaaatt gcttctaatt atccaagtaa cacactgaag tgtcaactta
     5821 tttgaataaa attattcttt ctttggacgt tctctgatac gtcttgccac aaatgaataa
     5881 agagcaaggc aaaaaattgc acccaataat gctggtatca aataaaaacc gccgagttcc
     5941 ggtcccagtg gtcctagtaa aaattcgccg agccaactac ctaaaaaacc gataatgata
     6001 tttccagata cgcctcccgg gacttccttt cctaaaattg cgccagctat caaacctaat
     6061 ataccgccga taaataacga ccataaaaaa ttcagcatct cctttccctc ctttatccat
     6121 atttctcact tttattttac gctttattca gaaggactcc aaaaatatgc gccaagaaac
     6181 tccagaaaaa aagaagttgt aacaaaagtg ttcagcttat ttccgtagaa gctgcttctg
     6241 gaacaccgtg aatcagaggt tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa
     6301 cggaaaaata gttcctcata ttttgccgtt ttttgagcta atatcgaggg acagctgatt
     6361 gaacatcgtt tattggagag gttgtaaaaa agctgttctg catcaagtaa taagaacagc
     6421 caaataaaaa cagctcctaa tgtttttaac ttggctggac ttattaccgc agatgcaagc
     6481 ttttgaacac cgtttattcg aaaaaaaagg actgtgacac ggcttttgtc acagtcccct
     6541 tttttcgaat aattattgtc cccctgtcac cttcacgatc acaagtacat tattcctatg
     6601 aattaaatta tttttcttca tttttcgttg aaccatcttc gttcaacatt gcttttcttg
     6661 acagatttac gcgtccttgt ttatcgattt cagttacttt cacaaggatc tcatcaccta
     6721 gtttaacaac atcttcgact ttattgacac gatcgtttgc taattgggaa atatggacaa
     6781 gtccatcttt tcctttgatc aagttgacga atgcaccgaa tttttcgata cggacaactt
     6841 tccctagata aacttcacca actttgactt ctttggtcaa atcttcgatg atcttgatag
     6901 cttttttgat catttctgca tcagctgaag caatacttac ttttccatct tgatcgatat
     6961 cgattttcac accagtttca tcgatgattc cgttgatcgt atcgccgcct ttaccgataa
     7021 catctttgat tttagcaggt tcgatctgga tcatttcgat ttttggtgca tattggctta
     7081 attcttcacg tggagcagca atcgttgaag tcaattcttc taagatttcc atacgtgctt
     7141 gttttgcttg agttaatgct tcagttagga tttgttctgt gattccttga atcttgatat
     7201 ccatctgtaa agcagtgata ccatcttttg taccggcaac tttaaagtcc atatcgccaa
     7261 gatgatcttc cagcccttgg atatcagtta ggatcgtata gttttcacca tcagaaacaa
     7321 gtcccatcgc aataccggcg actggcgctt tgattggtac acctgcatcc atcaatgcta
     7381 atgtgccagc acagatactt gcttgggaag aagaaccatt tgattccaat acttctgcta
     7441 ctaaacggat cgtataagga aattcttctt ctgaagggat tacttgtgct aacgcacgtt
     7501 cacctaatgc gccatgtccg atttcccgac gccctggaga gcctgcacgt cctgtagaac
     7561 caacagaaaa ttgagggaaa ttgtagtggt ggatgaatcg tttgctgact tccaccccta
     7621 aaccatcaat gatttggtgc tctcctagag gtgcgagtgt acaagctgat aaagcttgtg
     7681 tttgtcctcg tgtgaacaaa cctgatccat gtacacgtgg caataagctg acctctgatg
     7741 acaatggacg gatttcatcc aatttacgcc cgtcaggacg gatcttatcg atggtgatca
     7801 attcacgtac gacatctttt tcaagatctt ctgtgatttg tttaacttct ttcaacaagt
     7861 gatcttcatc ttcatgacct gcaaatttct cagcataagc ttcgcggata tcgatcttca
     7921 cctgctcgat attatcttcg cgagcaagtt tttcttcagt cattactgct gctttcattg
     7981 tagcgtaaga agcatcaaag atttcttttt tcaatacttc atcaacttgc agtaaagtca
     8041 cttccatttt ttctttccct actgcttgaa cgatttcttc ttggaaagca actaactctt
     8101 tgatagcgtc aaaaccaaac agcaatgcac caagcatgtc ttcttctgaa acttctttcg
     8161 cgccgctttc aaccatattg atggcttgtt tcgttccggc aacagtcaat tcgatatctg
     8221 tttgttctgc ttgttctact gttggattca agacgtactc gccatttaca cgaccaactt
     8281 ctacgcctgc gattgggccg tcaaatggga tatctgagat tgatagtgct aaagaagaac
     8341 ctagcattgc tgccatagct ggcgagcagt ctgtttctac actcatcacg atgttcgtta
     8401 cttggacttc attacggaag ccttctgcaa acatcgggcg aattggacgg tcgatcaaac
     8461 gggcagtcaa tgttgcttcc gtacttggac ggccttctcg tttgatgaaa cctccaggga
     8521 ttttccctgc tgcatacatt ttttcttcat aattgatcgt taatgggaaa aagtctgtat
     8581 cttttgcttc tttagaggcc acagctgcac ttaagactac tgtatctcca taacggacta
     8641 aaacggctcc gtttgcttgt tttgctagct gaccgacttc gatttgtaag ggacggccgc
     8701 cccatgtcgt tttgaatact tgtttttctg acataacttc tccttttcct tctcgtgaaa
     8761 gcaagacgct actaaacaaa tgaaaactgc cgaaagccgt cttttgagca gctttcattt
     8821 ggttagtagg agtccatcta gcttgatcac ggaattttac ttaattgata tacgagtaaa
     8881 aaaagtgaga ttcatgtgaa tcccactctt tttgactaaa attaacgacg caagcctaaa
     8941 cgtttgatca attcgcggta acgttgaacg tcagttttac gtaagtaagc taacaagtta
     9001 cggcggtgac caactttttt cattaggccg cggtatgaat gatggtcttt tttgtgcacg
     9061 cgtgcatgtt cattcaagtg gttgatttct tcagttaata cagcgatttg tacttctgga
     9121 gaaccagtat ccccttcatg gcgagcgtat tcgttgatga tttcattctt gcgttctttt
     9181 gaaattgcca tttctttcac ctctttcgat tattcccctg cgactgagta aaacgttggt
     9241 gattcgctta accaagtggc aggtcatgtg cctagagcac acagctatac tttacctgaa
     9301 accgggttga aaatcaagta gttaatcatt aagaaaaggt agttttcaaa caatcttact
     9361 actacttttc catttttgtg gtaaactata atgcgaaaat agacttgaaa cacagtcttt
     9421 taagaaaggg gtacatattc catgattaca atggatgata tcattcgtga aggaaatccg
     9481 accttacgcg aagttgcaaa agaagtttct cttccattaa gcgaagaaga tatctcttta
     9541 ggaaaagaga tgcttgagtt cttaaaaaac agccaagatc caatcaaagc tgaagaacta
     9601 cacttacgag gcggtgttgg cttagctgct cctcaattag atatttctaa aagaatcatt
     9661 gctgttcacg taccaagtcc tgacccagag gcagatggac cttctctaag tactgttatg
     9721 tataatccta aaattttgag ccattctgtt caagatgctt gtttagggga aggtgaaggc
     9781 tgtttatctg ttgatcgaga agtaccagga tatgttgttc gccatgccaa aatcactgtg
     9841 tcttattatg atatgaatgg tgaaaaacac aagattcgtt tgaagaatta tgaatcgatc
     9901 gtcgttcaac atgaaatcga ccatatcaac ggtgtgatgt tctatgacca catcaacgat
     9961 caaaatccat ttgcactaaa agaaggcgta ttggtgatcg agtagagaat tggcaggttc
    10021 cttcgatact atgctacaag aaagctttat aaaaaagggg agtgctcaaa agctgcactt
    10081 ccctttttta tgtttctcca ctaatcgaaa tttagataga catctagaaa tcgcaattcc
    10141 aaaaaattct gtgtattgga tacgcatctt ttcttatttt ttcttatttc ttccatatgg
    10201 gtgtttatct cagagatgat agaatagttt ctaggataaa aagtagcagc tacgatgtta
    10261 tccttcgtag aggattcctg cttcagctag tttttgtttg atgtttttca gcatatgctt
    10321 ttctgggtag cggatcgtat gcagatggat gccgcctgtt aattcggata acaaagtagc
    10381 attacttttt tgataacgtc tcataaagga atcgatatct tgtggcgttt tgatgtgcaa
    10441 agttcctgct aactcaccgt aaaggggatg ttccacaatt acatcaatga tttcaccacc
    10501 attttcaacg attagtctca gttcttcttc tgtttgatct tttccatgct ggacagcgat
    10561 tttactgatg tgcccagtat tggtttcttc tgtttcgagg atataacctc ttgctgttgc
    10621 cacgatactt ttacctgcag ctcgtagtaa agcaatatcg cctacaatga tctgacggct
    10681 gactccaaaa gttttcgcaa acttgctagc actcactggt ttttctgtat ttgccaaggt
    10741 ttgcatgatt tgttttcgcc gtgtctctcc ttcgatcaca cttattcctc cttcattttg
    10801 ttttggtatt cgcgcgagtg tttcatcaaa tcaacatacg tgtctgttat tgtttcctta
    10861 tatcccttat ttttcagcag actaacaact ttatccaata cttgctgctc tctttttgca
    10921 tcataaactg cttggttttc ttcttttttc acttgtccta ttttagttac cgtatccatt
    10981 cttttttcta ataaagcaac caactctcga tcgatcatat cgatcttatg acgtaatgtt
    11041 tcaagagact cttctgttag ttcttcctct actgtcttga tgatacgagc aggatttcct
    11101 gcaatcacta cattgtcggg aaaagatttg gtcacaaccg aacctgcgcc gactactacg
    11161 ttgtccccta gagtgacacc cggcgtgacg ataactcctc cgcctaacca aacattatta
    11221 ccgattttaa tgggttttgc gtactccaga ccgctgttac gtttcactgg atgtagtgga
    11281 tgcgtcgccg tgtaaagctg gacattcgga gcgaacatgc agttatcacc gatttcaatc
    11341 gtgctgacat ctaaaaatgt acaattgaaa tttgcataaa aattctcccc aacaaatata
    11401 ttatatccgt aatcaaaatt gataactggc tccatataga tttttttccc tgtacggcca
    11461 aaagtctctt tcaacaattg ggatcgcagt tccgctgatt ctgcttggtt gatgatttgg
    11521 ctttgacttc ttgcaaacat acggtcggca accagctcag gatcattggc aaaataaagc
    11581 tcgcctgcaa tcattttttc tttttcggtt ttgaattcag tcataatctg ctcctaattc
    11641 ttgtattttt ttgaaaacaa ccgctccgtc ttcttgaata aacgtataag gctccgctaa
    11701 ataaacaggc gtttgattga ttactaagat tttggctgac ggctggcgaa actgtaccag
    11761 atcacaaaac ggatgaactt gaaaactcgt tccaacaatg acgatcagct ctgcttggct
    11821 gacggcataa gccgcctgct ccatgactcc atcttgaaac ccttcaccat ataacacgat
    11881 gtctgggcgt atttgtcctc cgcatgcttg atgtttgtcg ctgtgaagat attctttcca
    11941 gtctaccgtt tgttgacatt ttcgacaatg acattgatac agactcccat gaaaattgac
    12001 gagttgcggg cttttagcct ttgcgtgcaa accgtcgatg ttttgggaaa caacccaaac
    12061 atttttttct ttcgctagat gtgctatttc caaatgaatg atattgggtt ttgcttcagg
    12121 atgataaatc tttttgacaa attgatagaa tttatccggt tcattcacca ttgcttgatg
    12181 actcagcaag tattccggct gctccatccc gtggtagatt cctgacaaag atcgatagtc
    12241 tggcactcca gaaggtgtgg atacccctgc accggtcaaa aatgtaattt gctgataagc
    12301 taatatctct tttgctgctt cgtctactgt taattgtttc atgttttgtc acctcacttt
    12361 ccattatagc ccatactcga acaaatttat tgttgaaaag tcagaaaaat gggaataaaa
    12421 aagtgttatg ggcatttgag caatttatca caatgttcac tgacaaaaga atgaaaacgt
    12481 gttacaatac tgctgtataa aagtgaggtg aatcgatatg ttaccattct ttatttattg
    12541 gggcgttatt ctggcttgta tcgcctggtt agcgttaagc gtttatttct ctgtttttta
    12601 tcttgtacgc aaagaaaatg gaaatctttg ggcttttgct ttattcaacg tgattgccgc
    12661 aattgtttta gccattacat tagccgtata tcgaacatgg ggctggggaa tcacccaata
    12721 ctcaagtttg atttatctaa tattagccat ttatggcgtc gttgttcttt tgcaagcgat
    12781 cctaggacgt gagccaaaga aagcggcagc ttaaatgatc gagatggaac tggacacagg
    12841 aaattttctg ttctagttct ttttttattg tattacatac atttttgtgt ataattatac
    12901 tatcgtttgc aatggataga tgattcacat tttgattgtt ttttcggtaa aagtaaagat
    12961 ttctatattt atcagtattt tttcaaaaac cagcagttat agattgacaa agcctctcga
    13021 gtatcgtaaa ctttttttgt ataaataact gaatacgaga tgcctcaatc aatgaggtag
    13081 aggtcgcgag aatcattaac cctgtggagt gaagcaaaca tggagaagca ggaataggga
    13141 ggatcgccga aatgtgcact tttttgcttg gagtgtatgt tgggacgcaa gttaagagct
    13201 tgcggactgt cttagcagtg atgctaagtt gcgctatgtt ttgtgagagg tttatcacct
    13261 tatggaattt tagcggcctt tttatatcta taaatcggtc gctttttatt ttttcagata
    13321 aagctttacc gcatttcatc tagaccgctc gtgtatcata taatcagaaa gggagaaaaa
    13381 aatgaaaaaa aatatggttt cagttgccgt actatttgct tttgtcttct cactattcag
    13441 tgtgggatct ctggcaaatg cagatgaaaa aacaccgagc gatgagtttc gcgtaggtat
    13501 ggaggctggg tatgcaccct tcaactggtc tcaacaaaca gatgagaata atgccttgcc
    13561 aattcaaggc caaaatagtt acgccggcgg ttatgatgtt caaatcgcta aaaaagtagc
    13621 agatggttta gggaaaaaat tagtcatcgt ccaaacgaaa tgggatggtc tggctcctgc
    13681 ccttcaatca ggcaagatcg acgcgatcat cgccggaatg agccctactg ctgaacgacg
    13741 aaaagaaatt gattttaccg atccttatta cgaatcacaa ttagtagtag tagttcaaaa
    13801 agatagcaaa tatgcaaaag caaaaagttt agcagatctt tctggagcaa agatcactgc
    13861 tcagttaaat acattccact atactgtgat cgatcagata ccagatgtgc agaaacaaca
    13921 agcaatggat aatttctcag caatgagaac agctttagct tctggaatga tcgatggtta
    13981 tgtcagcgaa cgtccggaag gtgtaacggc aactagtgtg aacaaagact tggaaatgct
    14041 tgaattcagc ggtgcagatg gcttccagac aaacccggaa gatgttcaga tttctgttgg
    14101 tatgcgtaaa ggcgacccgg atatccaaaa agtaaaccag attctttctg gcatctctaa
    14161 cgatgaacga accaaaatta tggaccaagc agtcaaagat cagcctgctg caactgatag
    14221 tgaagaaaaa cctggcatca tttctgattt cactacgatt tggaaacaat acggcaatat
    14281 gttcttacga ggtgctggac ttaccttgtt catcgcattg atcgggacag ttgtcggtac
    14341 tacacttgga ttactgattg gtgtcttccg ttcggtacct gaatcaggaa atgcactgac
    14401 tcgcttcttc caaaaaatcg gaaatgcact tctttctatc tatgtcgaag tattccgcgg
    14461 aacaccgatg atggtacaag caatggtcat cttctacggt ctggcactcg cttttggcat
    14521 ttcattgaac cgaacgattg ccgctttgtt tattgtctct ctcaatactg gagcttacat
    14581 gacagagatc gttcgtgggg ggatttttgc agtagatgaa gggcagttcg aagctgcgca
    14641 agccattggt atgacccatg gccaaacgat gagaaaagtt gttattccac aagttttacg
    14701 gaatatcttg ccagcaacag gaaatgagtt tgttatcaat atcaaagata cagcagtttt
    14761 aagtgtaatc ggcgtagctg atttgttctt ccaaggaaac tctgcttccg gtgcgaattt
    14821 ccaattcttc caaacattta cgatcgtcgg gatcatctac ttagtcatga cctttacgat
    14881 caccagaatc ctgcgggcgg tagaaaagaa aatcgatggt ccttcagctt ataccaagct
    14941 tgaagaaatc gacaattcaa atttgcagga ataaggagga agaatcatga atgaaaaaat
    15001 tattgaaatc aaacatctaa gaaaaagctt cggtgaaaat gaagtattga aagatatcag
    15061 cgtaacagtc aataaaggtg aagttgtgac gatcatcggt tcttccggtt ctggtaaatc
    15121 tactttcttg cgctgtatca atttactgga aaaaccaaca ggcggcgaga tcatttataa
    15181 tggagaaaat gtattagctc ctaaatataa tttacctaaa tatcgaacga atctaggtat
    15241 ggttttccaa tccttcaacc tgttcaacaa tatgaatgtc ttggaaaact gtatgtctgg
    15301 tcaaacaact gtcctgaaac gagataaaga aactgccaaa aaagtagcaa tcgaaaatct
    15361 ggaaaaagtt ggtatggatc gttatatcga agccaaacca gcacaattat ctggtggaca
    15421 gaaacagcgt gtcgcaattg ctcgggcatt atcaatgaat ccagatgtat tgctattcga
    15481 cgaacctact tcggcgttag accctgaaat ggttggcgaa gtgcttaata cgatcaaaga
    15541 cttagcacat actggcttga caatgcttat cgtgacgcat gagatggaat ttgcaaaaga
    15601 tgtatctgac cgtgtcattt ttatggataa aggtgtgatt gcagaagaag gtacaccaga
    15661 agatatcttt gtccatccga aagaagaacg tacaaaagaa ttcctttcac gtattttaaa
    15721 tgcttaggat caatagctga ttgggacaaa gagctgcggt gtttaccgca gttctttttt
    15781 atgaattact cataaaaggt aaatttctga aaattttgga atcttagttg tgtttttcag
    15841 aaaactctct taatatggaa gtaattaaac ttttttagtt aggatgattt aaaaatgaat
    15901 gaagagcaag aactgcagcg tggactgaaa aatagacatg tccaactgat ttctattggc
    15961 ggagcgattg gaacggggtt gtttttaggt tccggaaagt ctatccagct agctggccct
    16021 tcgatattgc tcgcctatct tattactgga tgcatctgtt ttttcattat gcgtgcgtta
    16081 ggcgaacttc tgttatcaaa caccaataac cattcttttc tggattttgt tgcagaatat
    16141 cttggcaaaa aagcggcttt catcactggc tggacgtatt ggttctgctg ggtatcgatt
    16201 gcaatggccg atttaacagc catcggaatg tatgtacgct actgggcgcc tggtgttcct
    16261 cagtggcttc ctgaactgat tgcattaggt cttcttcttt gcttgaatat ggtggctgtc
    16321 tcattgtttg gtgagttaga gttttggttt gcattgatca aagttgtagc gatcattgcc
    16381 tttatcctcg taggagctta tatgatcctc acccactata cgacagatat tggcacagcc
    16441 agtatcacga atctatggtc tcatggcgga ttctttccaa ctggtgcaaa aggatttatc
    16501 ttagctttcc aaatggtaac ctttgctttt gctgggatag aattagttgg attagtcgct
    16561 ggagaaacag aaaatcctga aaaagtgttg ccggaagcta tcaataatat tcctatcagg
    16621 atcatcttat tttacttagg atctcttttt gtcattatgg caatctatcc atggaacagc
    16681 ttgaatccag ataacagtcc atttgtagaa gtattttcgg aaattgggat cacgattgct
    16741 gcttccttga tcaatttagt cgtattaagt gcagcagctt ctgcctgcaa cagtgcaata
    16801 tatagtacag gacgtatgct tcgttcttta gcgcaagaag gcagtgcccc taaaaaattc
    16861 aaaaagttaa caacacatca tgtgccaggg aatgctttga cattttctac tatcgtcatt
    16921 ttcatttctg ttatcttgaa ttacgtgatg cctagtgaag tattcacact agtaagcagt
    16981 attgccacta cttgtttctt gtttatctgg agtatgcttg tctacacgca catgaaatat
    17041 cgtaaaagta tcctcggcaa aaaagctcat tcatttaaaa tgccgttgta cccatttagt
    17101 aattatctgg tatttcttta catggccttt gtgtgtgtcg tcttgttcct aggaaaagat
    17161 acgagaatcg ctttattact aacacctgtg tggttcttgc ttctattact aatctatcat
    17221 atgaaatatg aacgtaagaa gtaagaaagt agttgtgaca gaagtggtca gcttcgagaa
    17281 ataagaagga atttacgaaa attgcttctt aaatttttgt gaaatttcgg cttatttctt
    17341 ggagataaaa gcttctgtcc cagcctcttt tctatttccc cggaaacaac cgctgataat
    17401 aggcatctgc ttttttatag gaaaaaatcc aaataccaga tacaaaaaaa cgtccgaata
    17461 acccgatcaa cggtacaaaa aagacaccgg caatcaagca taagttaatc atcatggaga
    17521 taagacttcg acggtccttt gacaaaactc tcacgacatg ggaatctggt ttatttgctt
    17581 tcatgatttg cctcatcaag ataccaaaag aaaaactcca catcgtgtat actaagacat
    17641 aagccatctc aggccaaaaa gcttcagggt tacgcccgac ccaactagta acagaaggac
    17701 ataaagtcag ccaaaagagc aaaaagatat tcgaccagac cactcggtaa ttaattcgtt
    17761 ctgccatctg aaatatacga tgatggttca cccagatcac cgcaacaaat ataaagctga
    17821 tggtataagc taataatgta tttcggatga cccataaaga atgaaagcta gatgtattag
    17881 ggatcttgat ctctaataca ataactctca aaacaatggc taccactcca tcagtaaatg
    17941 cttctaatct cgactttgtc acgatgattc ctcctcagat gattcagcgc ttttatttta
    18001 tcacttacgt tattaaataa ccatcgtttt atatagggaa ttttctgaaa attgttgcat
    18061 atttttcgaa attttgttac ttttaaaaaa
//
LOCUS       DANJJH010000057        18043 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000057 DANJJH010000000
VERSION     DANJJH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(344..565)
                     /locus_tag="PWS53_001804"
     CDS             complement(344..565)
                     /locus_tag="PWS53_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872025.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(727..799)
                     /locus_tag="PWS53_001805"
     tRNA            complement(727..799)
                     /locus_tag="PWS53_001805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(764..766),aa:Lys,seq:ctt)
     gene            875..1105
                     /locus_tag="PWS53_001806"
     CDS             875..1105
                     /locus_tag="PWS53_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872026.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1149..2318
                     /locus_tag="PWS53_001807"
     CDS             1149..2318
                     /locus_tag="PWS53_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872027.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            2892..3461
                     /locus_tag="PWS53_001808"
     CDS             2892..3461
                     /locus_tag="PWS53_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL0872028.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            3448..4308
                     /locus_tag="PWS53_001809"
     CDS             3448..4308
                     /locus_tag="PWS53_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0872029.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            4498..5202
                     /locus_tag="PWS53_001810"
     CDS             4498..5202
                     /locus_tag="PWS53_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0872030.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5207..7042
                     /gene="walK"
                     /locus_tag="PWS53_001811"
     CDS             5207..7042
                     /gene="walK"
                     /locus_tag="PWS53_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL0872031.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7039..8355
                     /locus_tag="PWS53_001812"
     CDS             7039..8355
                     /locus_tag="PWS53_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872032.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            8356..9225
                     /locus_tag="PWS53_001813"
     CDS             8356..9225
                     /locus_tag="PWS53_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL0872033.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9280..10089
                     /locus_tag="PWS53_001814"
     CDS             9280..10089
                     /locus_tag="PWS53_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0872034.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10192..10821)
                     /locus_tag="PWS53_001815"
     CDS             complement(10192..10821)
                     /locus_tag="PWS53_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL0872035.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10991..12283
                     /locus_tag="PWS53_001816"
     CDS             10991..12283
                     /locus_tag="PWS53_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0872036.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(12359..13021)
                     /locus_tag="PWS53_001817"
     CDS             complement(12359..13021)
                     /locus_tag="PWS53_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0872037.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13246..13530
                     /gene="groES"
                     /locus_tag="PWS53_001818"
     CDS             13246..13530
                     /gene="groES"
                     /locus_tag="PWS53_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL0872038.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            13581..15206
                     /gene="groL"
                     /locus_tag="PWS53_001819"
     CDS             13581..15206
                     /gene="groL"
                     /locus_tag="PWS53_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL0872039.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(15295..16443)
                     /locus_tag="PWS53_001820"
     CDS             complement(15295..16443)
                     /locus_tag="PWS53_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872040.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(16545..17129)
                     /locus_tag="PWS53_001821"
     CDS             complement(16545..17129)
                     /locus_tag="PWS53_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0872041.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17256..17687)
                     /locus_tag="PWS53_001822"
     CDS             complement(17256..17687)
                     /locus_tag="PWS53_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0872042.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(17705..18025)
                     /locus_tag="PWS53_001823"
     CDS             complement(17705..18025)
                     /locus_tag="PWS53_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872043.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5987 a         3023 c         3601 g         5432 t
ORIGIN      
        1 taaaatttaa aattaactaa ctaaaaagtc ccctttccac ttattctgtt taaaagaatc
       61 ttctaatagt taaaggatta gtagattctt ttaaacagaa aacctattga tcattgaaat
      121 ttaaatacat tctatcagta cgttaacact tcaaggagca tattttagtt gcgccatgat
      181 ttcttactgt aaagaaggag cgataaacat tctaaaataa tttttatagt caataaaaag
      241 cgatgatttg aagttgggat aatgatactt ccgtaaggct cgccgtaaac ttggagagag
      301 gtgaaattcc tatgtgcttt tgacaaaagc agctgattaa gaattatttc catatgttga
      361 tttcaacatt ttttctatta tttttaagac caaattcagt gaggcaaata gcatcatgta
      421 aatttagttt ttctattgaa cgttctccac tttttagttt ttttatagta ctcaaattta
      481 atcctgtatg tttagagatc tcatttgcag tatggttttg taaagtttct cggattattg
      541 ttatattagc aatttcatta cgcataatta tgttcctttc ttatcaaaaa tatcaatgac
      601 tacagtgggc ttttagtgaa aaaaaggggt actacagtct cttttgaaaa caaaaaaata
      661 cagtatatac gttttctaaa tgctaagcac caacgtttac actgtattta agggtttggc
      721 acccaatggg ctgtgagggg ctcgaacccg cgacccgatg attaagagtc atctgctcta
      781 ccaactgagc taacagccca aattaatact ttgttattct aacacctaaa ataaaaaagt
      841 gcaatactta ttatcagaat tgagagtgaa ttttatggat tttcaagatt atgaaaaaat
      901 atttgaaagt tatccatcga tattgaaaat tactgattta actaaaatgt taaatattgg
      961 aaaaaataaa gcttataatt tgctgaaaga tagatcaata gaaaatataa aaattggagc
     1021 aaggtacaaa atacctaaaa aagctgtcat tgagtatata ttgaaaatac aagatgaagg
     1081 taaaaatcat gctataataa aatagtcaaa atttttgccg atatttcagt ttaagaggag
     1141 agattgaatt gaaattaaga gcaaacataa ttgaaaaaag aggaacatta caagtagttg
     1201 tatcttataa agactctaac ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa
     1261 agaaaggaaa taaaaaacga gcagaaaaaa ttagagatga atttttacta gaattggaaa
     1321 gtcaattagc tatggaatgt gaatgtgcta atccaaattt attgttttat gactatttgc
     1381 ttaattattt ggaagtatca aaaaaacaag tagcatttaa cacatatgtt gggtataagc
     1441 attacgtcca taatcgaatt tacaagtttt tttatcctaa aaaaattaaa ctaaataggt
     1501 taaagcctta tcatttacaa gagttttatc aatatatgtt aaatgataac tgtacagcaa
     1561 atacggttat tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag
     1621 aattgataag tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt
     1681 ctcaagttta taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac
     1741 tctatttggt agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat
     1801 taaaatggtc tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag
     1861 aaaatccaga gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct
     1921 atcgtacatt acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc
     1981 aacaagataa tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat
     2041 ttactatgga agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt
     2101 tgattaagaa aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt
     2161 caataatgtt agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat
     2221 cgtattcgac aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag
     2281 cagaagcatt agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt
     2341 ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc
     2401 aatgaaaaaa ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa
     2461 atagagcatt tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa
     2521 gtacttaata atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca
     2581 gaaaaatctt tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt
     2641 atataagcat accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat
     2701 tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa
     2761 gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt
     2821 aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag
     2881 gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa
     2941 cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca
     3001 gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta
     3061 cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa
     3121 gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga
     3181 ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag
     3241 cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac
     3301 aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa
     3361 gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa
     3421 ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat
     3481 tcaggtacgg atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg
     3541 aaaattattt ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc
     3601 tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca
     3661 gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc
     3721 agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg
     3781 gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa
     3841 gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag
     3901 ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt
     3961 ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc
     4021 tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt
     4081 ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta
     4141 gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga
     4201 gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac
     4261 actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg
     4321 aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata
     4381 aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa
     4441 agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg
     4501 aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta
     4561 gccaaagaag gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt
     4621 gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta
     4681 gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa
     4741 gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag
     4801 ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg
     4861 gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc
     4921 catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa
     4981 ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta
     5041 ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta
     5101 cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt
     5161 cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt
     5221 ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga ttttattgat
     5281 ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac
     5341 atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt
     5401 gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga
     5461 aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg
     5521 aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat
     5581 caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat
     5641 caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa
     5701 aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc
     5761 ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac
     5821 acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg
     5881 gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt
     5941 agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag
     6001 cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac
     6061 gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc
     6121 gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc
     6181 aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg
     6241 agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt
     6301 gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt
     6361 ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga
     6421 aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac
     6481 agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa
     6541 tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg
     6601 gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca
     6661 aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat
     6721 gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac
     6781 gcataataac gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct
     6841 gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg
     6901 gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg
     6961 ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta
     7021 tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg
     7081 atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt
     7141 tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact
     7201 tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc
     7261 aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag
     7321 ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct
     7381 ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat
     7441 ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata
     7501 caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat
     7561 gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca
     7621 aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat
     7681 tcgatcaaac tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga
     7741 aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt
     7801 ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt
     7861 ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt
     7921 agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc
     7981 gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt
     8041 ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg
     8101 aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa
     8161 atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt
     8221 ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg
     8281 tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga
     8341 ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta
     8401 gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc
     8461 attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac
     8521 acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat
     8581 cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt
     8641 gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac
     8701 gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat
     8761 attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca
     8821 ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa
     8881 atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg
     8941 catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca
     9001 atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag
     9061 ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat
     9121 gtagcgatcg aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat
     9181 acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat
     9241 gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct
     9301 tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa
     9361 aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct
     9421 caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat
     9481 catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag
     9541 cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta
     9601 tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc
     9661 catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg
     9721 ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt
     9781 tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat
     9841 ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc
     9901 atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa
     9961 aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt
    10021 ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac tgagctattt
    10081 tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt
    10141 tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt attatcgact
    10201 gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat
    10261 cgcgtaatct gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc
    10321 acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat
    10381 ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa
    10441 agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc
    10501 tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc
    10561 aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc
    10621 gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa
    10681 atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt
    10741 cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc
    10801 tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt
    10861 tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg
    10921 ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag
    10981 gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt
    11041 gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat
    11101 tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat
    11161 agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat
    11221 agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg
    11281 cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac
    11341 tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta caactgggct
    11401 catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga
    11461 gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt
    11521 cacttttacg cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca
    11581 aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt
    11641 gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat
    11701 ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt
    11761 agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct
    11821 cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac
    11881 ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt
    11941 tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc
    12001 tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt
    12061 tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc
    12121 gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag
    12181 ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag
    12241 acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct
    12301 gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt
    12361 atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt
    12421 gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac
    12481 ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc
    12541 tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc
    12601 ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg
    12661 cttccatgat catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca
    12721 atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca
    12781 atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg
    12841 gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg
    12901 cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg
    12961 gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca
    13021 tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc
    13081 aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa
    13141 agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt
    13201 aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta
    13261 ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt
    13321 ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc
    13381 cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg
    13441 ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca
    13501 ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata
    13561 atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag
    13621 ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag
    13681 gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga
    13741 cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat
    13801 cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc
    13861 tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag
    13921 gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct
    13981 caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg
    14041 ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca
    14101 caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg
    14161 atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag
    14221 aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat
    14281 tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg
    14341 gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg
    14401 taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga
    14461 caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa
    14521 acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt
    14581 ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa
    14641 caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg
    14701 tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca
    14761 ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg
    14821 gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg
    14881 caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa
    14941 acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg
    15001 gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa
    15061 ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga
    15121 cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc
    15181 catcaatgat gggcggtatg atgtagtaat taagaaagaa ccctatttta acagggttct
    15241 ttcttctttt tttgttttat tttaagcaaa aggggcaaag aaggggcaaa aatatcaaag
    15301 gctatttagt ttatcaatta cttgttgttt ggctcttttt gttacatggt tataaataga
    15361 cagagttgtg tttgcatcag aatgccctac acgctccata atggctttaa gaggtacgcc
    15421 taattcagat aatagtgaaa cgtggctatg tctgaatata tgtgaggata gacttttttc
    15481 taattccaac tcttcctcta ctttatggag tatagcgtta aatgagtgca gcgcaagcgg
    15541 agtgccactt gtagatataa atatatattg atcgggatct gtgggtctac ctgcaagaat
    15601 attgtcagct atcacacttt caatcaattc ttttgcacga ttgggtaatt gcacttcacg
    15661 ttgcgaataa gtattttttg gagttgtttt tatagcatta tccattttca cagatgtgta
    15721 atctaaggtc ccattaatgg aaattttccc atcctcatag tccttcattt gcaaagctag
    15781 caattcccca tatctcaaac cagttaaata tagaaactca gctattatgc cgtgtagttt
    15841 tctgcgagga ttggagtata gctgtttcag tatttgatca atttcttctt tctccagata
    15901 ttttttattc atagaaagcc ttcttttttc ttcttccact tttttaggat ggattttaac
    15961 tgctagcgca gggtttcttt gaatgtattt tctatcgatt gcatagttta gcataacaga
    16021 tagagttgtt tttgtttgtt ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc
    16081 ttcaattatc ttattaatga gtgtctcatc aatgtttcta acgatcgtat catcgcttat
    16141 gtgcttagaa acatgtttca tcatcattgg aaccttcaaa tagctagtac gtttaacatg
    16201 ctgcttataa tattcatacc attctttata cagctcacca aaagtgatat ctgatttatt
    16261 gtaatcttcg agtgcttctt taattttttt atctagaatt ttctgagctt ttttccacgc
    16321 ttgtggtgaa ttacttgtaa gtgttgtaga tttttttcgt gttttttctg tataaggatc
    16381 tacatatctt tcaataaact tgaattggcc atctttggtc tgttcaaccc acacttttaa
    16441 catctcctat catttgctat aataggcata acaaatagac ctatataggt ttgttttcta
    16501 aaagcacgct cttactctgg acggtggggc gtgtttttta tttattatac agttgcttta
    16561 aaaatagctg ctttttcaaa tgatactaaa ggagagaagt ggatttctat ttctcccttt
    16621 tggtttaacg tgaaatgagc agttacatcc atttgttttc ctggagcaac agatcccatt
    16681 gtattttcgt tggcatatgt ctctgatttt ttgtcatctg gtccatatac ttccacatct
    16741 gtacctacag ggatatctga atcgccatca ttttttacgg tataagtaat ttttactact
    16801 tgtgcaggtt ggttttcttc aaattgattt ctttcatcag ttagttctac actatttagt
    16861 gtatattcag catcaccaac ttttacagtg tcaccaatct tatagaaggt atcgcttttt
    16921 tcttctttag aagatgaagc agaggttgaa gatttagtta ctttttcgcc accattatca
    16981 cttgctttat tactatctga tccaccattt aaagcagaac caataattat aattaaaatt
    17041 actgctaata cccaaaacca tacttttttg taaaaaggtt tactaacttt atacattttt
    17101 ccgtcttgac ccataacttt ttttgccatt taaatattcc tcacttcttg ttataatata
    17161 tttgcgatct cagaaatgag gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt
    17221 gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa
    17281 tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga
    17341 atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc
    17401 tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt
    17461 gagtgaaaag ttgtagaagt atcgtacaaa actgaatatt caaaatgaga agcggtgaaa
    17521 tgcgcaagtt catgagataa atgaaaggct tctgcagttt cgccatatag attttcattc
    17581 aaaaaaatga ttctaggctt cggataatag aagccaggct ctttcatttc catgtaaact
    17641 actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca acccatcact
    17701 ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct
    17761 tagatatttc ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt
    17821 taggggtttc tctttcttct ctacctagaa ggtagtctac agagactcca aagtaatcag
    17881 ctaaccgagc taactcaata gaattaggag tttgtctttt ccaacttcct aaatatccat
    17941 tcgaataccc gaaattcatt tcgagctgtc taatggataa tcctttttct tttgctaact
    18001 cctttattat ttcgtaagta ttcattgata aatcaacctt tct
//
LOCUS       DANJJH010000058        17889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000058 DANJJH010000000
VERSION     DANJJH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(45..1511)
                     /locus_tag="PWS53_001824"
     CDS             complement(45..1511)
                     /locus_tag="PWS53_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL0872044.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="PWS53_001825"
     CDS             1898..2494
                     /locus_tag="PWS53_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0872045.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="PWS53_001826"
     CDS             complement(2552..3034)
                     /locus_tag="PWS53_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872046.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="PWS53_001827"
     CDS             complement(3140..3337)
                     /locus_tag="PWS53_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872047.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="PWS53_001828"
     CDS             3632..4390
                     /locus_tag="PWS53_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0872048.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="PWS53_001829"
     CDS             4450..4878
                     /locus_tag="PWS53_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872049.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="PWS53_001830"
     tRNA            4924..4996
                     /locus_tag="PWS53_001830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="PWS53_001831"
     CDS             complement(5146..5517)
                     /locus_tag="PWS53_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0872050.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="PWS53_001832"
     CDS             5732..5917
                     /locus_tag="PWS53_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872051.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="PWS53_001833"
     CDS             complement(6000..6188)
                     /locus_tag="PWS53_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872052.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="PWS53_001834"
     CDS             complement(6212..6475)
                     /locus_tag="PWS53_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872053.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="PWS53_001835"
     CDS             6633..6890
                     /locus_tag="PWS53_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL0872054.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="PWS53_001836"
     CDS             complement(6961..7152)
                     /locus_tag="PWS53_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872055.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="PWS53_001837"
     CDS             complement(7293..7613)
                     /locus_tag="PWS53_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872056.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="PWS53_001838"
     CDS             complement(7636..8070)
                     /locus_tag="PWS53_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0872057.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..9043
                     /locus_tag="PWS53_001839"
     CDS             8405..9043
                     /locus_tag="PWS53_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0872058.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9367..9450)
                     /locus_tag="PWS53_001840"
     tRNA            complement(9367..9450)
                     /locus_tag="PWS53_001840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9414..9416),aa:Leu,seq:cag)
     gene            9978..10478
                     /locus_tag="PWS53_001841"
     CDS             9978..10478
                     /locus_tag="PWS53_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872059.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10668..11012)
                     /locus_tag="PWS53_001842"
     CDS             complement(10668..11012)
                     /locus_tag="PWS53_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL0872060.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11166..12086)
                     /locus_tag="PWS53_001843"
     CDS             complement(11166..12086)
                     /locus_tag="PWS53_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0872061.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12273..12764
                     /locus_tag="PWS53_001844"
     CDS             12273..12764
                     /locus_tag="PWS53_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL0872062.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12788..15163)
                     /locus_tag="PWS53_001845"
     CDS             complement(12788..15163)
                     /locus_tag="PWS53_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL0872063.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15489..16115
                     /locus_tag="PWS53_001846"
     CDS             15489..16115
                     /locus_tag="PWS53_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0872064.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16138..16842
                     /locus_tag="PWS53_001847"
     CDS             16138..16842
                     /locus_tag="PWS53_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0872065.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17034..17867
                     /locus_tag="PWS53_001848"
     CDS             17034..17867
                     /locus_tag="PWS53_001848"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL0872066.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5553 a         3489 c         3015 g         5832 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     8821 aagtacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     8881 aacgaacgga cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta
     8941 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     9001 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg
     9061 tttaaacaca ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt
     9121 tttgagttta agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag
     9181 aatctataaa tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac
     9241 aaattttttt tgatttattt gaatctggtt ccgttgttta aaatttttcc tatattcttt
     9301 tagataatat gttattttct ttctcatcgc tccaggtgta ccttctcttt ataaaaaaac
     9361 aaattatgcg gctaagaaga ctcgaacttc cacgaggtca ccctcactag cgcctgaagc
     9421 tagcgcgtct gccaattccg ccatagccgc atcacatctt ttagtttagg attacatgtg
     9481 ttttttgtca agaacatatt tttgttcttt ttcatttctg tacgccctcc cttatgccat
     9541 ctacacaaat aattcatccg agcaactcca tcatctattg agggttggtg aaaaaatctg
     9601 ttttatccat atgatcgatt tcaatgttaa acgaacgact ctgggattag taactatttt
     9661 atttttttct tcatttcttt tttcctcttt ttctcgattc agctatcttc tttcatttgg
     9721 atattgatta tttcttctgt tgattactat ccttctggaa ttctagacgc tgaagtaatt
     9781 cacgttttct ttcttctaga gatcataagt aacgaatgaa cgaacttcaa ataatttttt
     9841 tattgtaatt catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa
     9901 aaataggcat ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac
     9961 ttaaagaggt gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt
    10021 atcagctaca aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct
    10081 atacatttgc tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg
    10141 atcttcctga gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt
    10201 atgtaggaat ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc
    10261 atattggtta cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag
    10321 cattagtact acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata
    10381 aaaaaaatct tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag
    10441 atgagaaaga atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa
    10501 agggatttga atgtccttta tcaatagaac acatttattt aattaactta taatatagca
    10561 gacaaaattt tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc
    10621 ttttttgttt ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc
    10681 aacgcaatcc gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt
    10741 ttgacttctg ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt
    10801 aaaatatctg ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca
    10861 ttttcaggat tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat
    10921 tcaggatcaa tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct
    10981 tgtccagacc gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta
    11041 tttatcatac tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt
    11101 aaatttataa aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg
    11161 attcctcatt ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac
    11221 attttttgtt cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg
    11281 ataaatgcat cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg
    11341 gtgatgacca tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt
    11401 aatgtcattc cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg
    11461 gtaacgatca ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc
    11521 attgcataaa tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg
    11581 atgactacaa tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt
    11641 tgtgaaagta caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat
    11701 aatcctacgc caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca
    11761 gtgatctgtc cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa
    11821 ctaaggatcg ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc
    11881 aaaagaaaaa taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag
    11941 gaaatgattt gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa
    12001 taatttaaag cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat
    12061 ctttcatttt tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg
    12121 tattccaaat gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga
    12181 gaagaatttt agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg
    12241 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
    12301 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
    12361 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
    12421 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
    12481 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
    12541 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
    12601 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
    12661 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
    12721 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
    12781 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
    12841 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
    12901 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
    12961 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
    13021 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
    13081 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
    13141 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
    13201 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
    13261 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
    13321 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
    13381 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
    13441 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
    13501 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
    13561 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
    13621 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
    13681 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
    13741 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
    13801 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
    13861 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
    13921 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
    13981 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
    14041 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
    14101 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
    14161 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
    14221 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
    14281 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
    14341 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
    14401 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
    14461 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
    14521 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
    14581 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
    14641 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
    14701 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
    14761 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
    14821 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
    14881 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
    14941 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
    15001 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
    15061 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
    15121 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
    15181 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
    15241 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
    15301 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
    15361 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
    15421 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
    15481 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
    15541 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
    15601 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
    15661 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
    15721 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
    15781 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
    15841 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
    15901 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
    15961 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
    16021 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
    16081 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
    16141 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
    16201 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
    16261 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
    16321 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
    16381 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
    16441 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
    16501 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
    16561 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
    16621 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
    16681 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
    16741 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
    16801 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
    16861 taccagagaa tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg
    16921 tgtacttatc tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct
    16981 caaaaaataa cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt
    17041 ttgcaaatat tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc
    17101 ccatcagtag tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt
    17161 cacaatttat gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg
    17221 ttctttattt tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag
    17281 atacgtggac actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt
    17341 tgcctttgga tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt
    17401 tgatcgttta cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc
    17461 gttggacttc tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag
    17521 tcttggcact tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga
    17581 ttggtgcttc gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt
    17641 ttggcgcaag cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg
    17701 aagaaactgt tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta
    17761 tcaaattctt actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta
    17821 ttatccttgg tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac
    17881 acttctctt
//
LOCUS       DANJJH010000059        17700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000059 DANJJH010000000
VERSION     DANJJH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            67..1023
                     /locus_tag="PWS53_001849"
     CDS             67..1023
                     /locus_tag="PWS53_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0872067.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1096..1212
                     /locus_tag="PWS53_001850"
     CDS             1096..1212
                     /locus_tag="PWS53_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL0872068.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1267..2949)
                     /gene="recN"
                     /locus_tag="PWS53_001851"
     CDS             complement(1267..2949)
                     /gene="recN"
                     /locus_tag="PWS53_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL0872069.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2967..3416)
                     /locus_tag="PWS53_001852"
     CDS             complement(2967..3416)
                     /locus_tag="PWS53_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0872070.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3523..4341)
                     /locus_tag="PWS53_001853"
     CDS             complement(3523..4341)
                     /locus_tag="PWS53_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL0872071.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4350..5240)
                     /locus_tag="PWS53_001854"
     CDS             complement(4350..5240)
                     /locus_tag="PWS53_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0872072.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5242..5466)
                     /locus_tag="PWS53_001855"
     CDS             complement(5242..5466)
                     /locus_tag="PWS53_001855"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL0872073.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5471..6808)
                     /locus_tag="PWS53_001856"
     CDS             complement(5471..6808)
                     /locus_tag="PWS53_001856"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL0872074.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6809..7663)
                     /locus_tag="PWS53_001857"
     CDS             complement(6809..7663)
                     /locus_tag="PWS53_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL0872075.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7760..8209)
                     /gene="nusB"
                     /locus_tag="PWS53_001858"
     CDS             complement(7760..8209)
                     /gene="nusB"
                     /locus_tag="PWS53_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL0872076.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8202..8627)
                     /locus_tag="PWS53_001859"
     CDS             complement(8202..8627)
                     /locus_tag="PWS53_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0872077.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8873..9937)
                     /locus_tag="PWS53_001860"
     CDS             complement(8873..9937)
                     /locus_tag="PWS53_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0872078.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10109..10354)
                     /locus_tag="PWS53_001861"
     CDS             complement(10109..10354)
                     /locus_tag="PWS53_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0872079.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10493..10867)
                     /locus_tag="PWS53_001862"
     CDS             complement(10493..10867)
                     /locus_tag="PWS53_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL0872080.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10950..11864)
                     /locus_tag="PWS53_001863"
     CDS             complement(10950..11864)
                     /locus_tag="PWS53_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL0872081.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11885..12688)
                     /locus_tag="PWS53_001864"
     CDS             complement(11885..12688)
                     /locus_tag="PWS53_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL0872082.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12721..13710)
                     /gene="manX"
                     /locus_tag="PWS53_001865"
     CDS             complement(12721..13710)
                     /gene="manX"
                     /locus_tag="PWS53_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL0872083.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13813..14304)
                     /locus_tag="PWS53_001866"
     CDS             complement(13813..14304)
                     /locus_tag="PWS53_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872084.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14495..17302)
                     /locus_tag="PWS53_001867"
     CDS             complement(14495..17302)
                     /locus_tag="PWS53_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0872085.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
BASE COUNT         5039 a         3923 c         2924 g         5814 t
ORIGIN      
        1 acacattcca tgtattccaa ttccctatcc gttataataa aagaaacagg caacaggagg
       61 aaaatcatga tcaaatattt tacattagat aatgagaaac tgcgtaatac cggaaaggaa
      121 actgaccaaa cgatctggta ttgtgtagaa cgtcctaatc aagaagagat tcatcagctg
      181 acacatcaat ttgaaattcc gtcagattac atcacttcca tattggacga tgcagaaaat
      241 tcccgtacag aagaattcaa tcaatcaaag ttgacaaagc cagcgttatt attgttacaa
      301 tatccttttg ctaaaacaag tcctagcggt tattttcaag tggatacata cccgctctct
      361 attattttga cgcccaaaaa gaagcttatc acaattacga atcatgaacc actgttttta
      421 aagaaagtct ttagccagac ctttccaaaa agtgatattt cgcctaatct caatatcttc
      481 tttcaattca tttggcaggt cacactctct tacaacagtt atttagctac gcttcaatct
      541 caatgcgata ctttggaagc caaacttcaa gtatccactg aaaacaaaca actttatcaa
      601 atcatggata tccaaaaaag cctcgtttat ttcagagaag cgacttctgc taatttagat
      661 gcattgaaag aactagcgca aaataaactt ttccaagaaa atcatgcttt gaaaaatcat
      721 ttacgtgatg tattggtgga aacagagcag gcaatgacta cttctcgcat tcaattaaaa
      781 ctagtagaac aaatgaatca gactttttca gcaatcgtat cgaataatct gaataatatt
      841 atgaaaattt taacttcttt aacgattatt ttaactatac cgacaatcgt cggcagtatc
      901 tatggaatga acattaagtt acctattgct gaaagggatg atgcctttat atggctagtt
      961 ctattaacat tatttatttg tatcgtaact acctattatt taaaaaaagg gaagttttta
     1021 taaaaaagcc ctcttgtgga aatggaaaaa agattgtaca cttgaatcaa ctaattaatg
     1081 acgaggttga aaacaatgcg tgaaaaaaaa caaacaagta cttttgctaa agtaacaaaa
     1141 tttgtcattt ggacaatgct gattttaacg attggatctg ttgtattgac tgctgtaatg
     1201 agtgtcatgt aaaaataaaa tatagctatc agctgctgaa aaggacctga accactgcga
     1261 ggtggctcag gtcctttttg cgagtttcaa tagttctttt gcatgttcga gtgtgagttt
     1321 tgtaatctca cttccagcca gcatacgtgc aatttcttct tctctttctt ttgttttcaa
     1381 ctctgaaaca gaagtttgcg tacgtccatc aatcactgct ttcacaatat aatattgata
     1441 gtcggcgact gcagcaactt gaggcagatg agtgatgcac aaaacttgag agtatttcga
     1501 tatttgagaa attttatctg cgatggcctg tgcaactctt ccgctcacac ctgtatccac
     1561 ttcatcaaaa ataatacttg tcacaccttg ctcagaagaa aaaatagttt tcaaagcaag
     1621 caacatccga gacagttcgc caccagaagc gactttcacc aaaggcttca gtggctctcc
     1681 tggattggtc gtaatgtaaa attcgatttg gtcaaatcca tcatgattca attgtctatt
     1741 ttcttctttt aagaaacgta cctcaaactc agtgttttcc atatacagac ttttcaattc
     1801 atgcagaatg gatttttcca attcttttgc gattttttta cgcgcttcat gaagatctgc
     1861 ggcttgttgc tgaagtagaa gctctttttg ctctagttcc tctttcattt tgtccaattg
     1921 accttctgaa aaatcagaag aagctagctc ttcggtgatt tcgtcatagt aagccaatat
     1981 tgactcaatg gattcgccgt atttacgttt caactgtcgg attaattcta aacgttgcgt
     2041 aacttcttct aaacgactct catctaactc caataaatca atctgacgac tcatatcgcc
     2101 gattgcatct tgcagcaaat agtaagcact ttggatattt tcataaattg cttcatactc
     2161 cacatctaga tgggcgatcc cttgcagttc tgaaacggcc aagccaacac catctacact
     2221 gttttgttca ttattcgtca gtgctccgta acctgcagct aatccatcag caattttttg
     2281 ataattgcta agtttttctc tttcttcttt taatttctct tcttctccga cttgcaaatc
     2341 agcagcagct atttcttctt gctggaaatg gagcatatct attctctgaa cataggattt
     2401 ttcattttgc tgcacttttc gtaccctttt ttccagctca cgataagaga tatattcttg
     2461 ctgataactc tttttcttct gttggaaagc tacatctcca aaacgatcca ataaagcaag
     2521 atgtgattca ggctggagca gttcctgatg ttcgttttgt ccttggatat ctactaaata
     2581 actgccgatt cttctcagat tagctaatgt gataatatga ccattcactc gacaaacatt
     2641 ttttccagtt aaggacatat cccgtcgaac aatcagattg tcctcatctg tttcgatccc
     2701 taattcaacc atcagttcag agaatccttc ttgttttggt agttcaaaca acccttcaag
     2761 cacacatttt tctgctcctt gacgtatata atcgcttgaa cctcttcctc ctgctaacag
     2821 acctaaagca tcgataataa tggatttacc cgcaccagtc tctccggtca gcgcggtcat
     2881 tccttcgtga aaagaaagcc gcaattccgg aataatcgca aagttagtaa tactgatttc
     2941 ttgtagcatc acattcgctc ctcaacttaa aggtaatgca ggaagtcttt cttcaataaa
     3001 actgcctggt cttcagttcg tgtaatcatc aaaacattat cgtcatcatt gataatcgaa
     3061 aataattctt tcttgtaacg tttatcaatc aaatttgctg ctgccgaagc atttcccggc
     3121 aatgttttca aaataacaaa tttttccatt tggtcaactg ccacgaaagc gtcttttaat
     3181 agtttttcca atttagtatt tacatcttcg tttgtttcag ctggtatgct ataacggtag
     3241 ccgccatctg aagccggtac cttgatcagt ttcagttcct ttatatctct ggaaatcgta
     3301 gcttgagtaa cagatattcc tttgcttttc aggatttcaa caaattcttc ctgctttcgg
     3361 atatcttgct cactcaacaa tcgagtaatc aaacgatgcc gatctttttt tctcattacg
     3421 tcttcgctcc ttagcatatt cttcttaatc aatgatagct tatttccttg gtataataaa
     3481 gaagaatccg catacttctc acttgatttt cacagttaca atttatttat ggtcaaactg
     3541 ttcgtgtgca tttgaaacca cttctgccat attaatagct gaattgattg ttccgctttc
     3601 gggtactttc ctcaagtgag ccaagaactc gatatttcct tctccccccg tgatcggaga
     3661 ataacttaat tccagcacat catatccacc ttgtgccgca aatgacaaaa tgtcttctaa
     3721 aactttttga tgggtctgag ggtcacgaac aattccgttt ttccctacag cttctctgcc
     3781 tgcttcgaat tggggtttga ttaaagcaat cacttctccc tcttctttta aaatagcatg
     3841 taatggaggc agcatcagtt ttagtgaaat aaaagaaaca tcgataacag caacttctgg
     3901 aattcctaag gtaaagtctt cgggttttgc ataacgaaaa ttcactcttt ccattacgac
     3961 gactctagga tcttgtctta ttttccaagc tagctgattg tagccaacgt ctaaagcata
     4021 actcattttt gcgccatttt gcaaagctgc atcagtaaat ccgccagtgg atgcaccaat
     4081 atctaacaat gtttttcctt caacagatag atcaaatgct ttgagtgctt tttctaattt
     4141 taatccccct cgagaaacat aaggaagttt tttacctttg actcttaatt cactactcac
     4201 tggaattttt tctcccggtt tatcaaaccg ctcgtttttt tcgttgtaaa tcaaaccagc
     4261 catcactgaa cgtttggctt gttctcttgt ttcaaataat ccttgtttga ccgctaatac
     4321 atctacgcgt tctttttcca ttctttccct catttctcca catcaaattg cttgatcatc
     4381 tggcgcatca gcccgccatc aaaagaaaaa acgtcctctt ctaatttgtc tatgatcttg
     4441 ttggcggata acagctcaat ctccaacgcc tcacgtgtct tttctaggcc taataaacgc
     4501 gggtaagtat ttttttccat acgttcatct ttaccagctg ttttccctaa tgttttcgtt
     4561 gtaccaatca catcgagtag atcatctctt atctgaaaag ctaaacctaa atgttcggct
     4621 aatcgttgaa gtagcaataa gatttcttca ggttgcttcg ctaatatgcc accagccagc
     4681 aaggcataac gaatcaaatg ccctgtcttt ctttcatgaa taaatgctaa ttcttctaaa
     4741 gaaagctttt tcgattcgcc ctcgatatct gcagcttgcc cagcaaccat accttgtgat
     4801 cccgcacaaa cagctaattg ctgaagcagc agaagcttag gagaattacc taagtgtgcc
     4861 atactgatta gctggaatgc tcctgtaagc aaaccatctc cggccaatat agctaatgct
     4921 tcgccgaata ctttatgatt ggtaggtttt cctctacgaa gatcatcatt gtccatcgca
     4981 ggcaaatcat cgtggatcaa ggaatacgta tgcaccattt ctaaagcagc tgctacttga
     5041 tagacgggaa catcaatagg ctcatcaaag gaagcaacgg ccgttaaaag taataaggga
     5101 cgtattcttt tcccgcctgc tttcactgaa taactcatcg catcatagag tcctctatcc
     5161 gttgtatgct catctaaaaa agacaagatt tctttttcta cagcaggtag atgaatagct
     5221 gaaaagtcag ttaatctcat ctcagctttc ctcgtttccg tcaaaatcaa cttcttcatt
     5281 tgattctgtc atcattttcg tcaaggtctc ttctgcttta gctaatgtat cttggcaatg
     5341 tttgcttaat tccattcctc gtttaaacga atctagagct gattctaatg gaacgtctcc
     5401 ttgttctaac tgcatgacaa ttttttctaa ttcttgcagt gactcttcaa aagttggatt
     5461 ttccatattt ttattctcct ttgtctatct cttgtacttg tcctttaacg attccatctt
     5521 gataatggat cgtcagcaaa tccccttgat gaatctcggt gacacttttt actacttgat
     5581 catttaacgt cgtatagctg tagcctcttc ccatgatttt caatggactc aacaaatcaa
     5641 gagattgaac agcttgagca aaacgctgct gctgttgttt catatattgt tggattttct
     5701 cttctaagcg tttttctaga taagcagttt cttggcgagc tgctttaact cgataaagcg
     5761 gagccacttg ttccaattgg tggcttaatg cgatcgtttg tttttctttc gcatgataaa
     5821 tttgctgtgt agcttgatat aatcgctgtt tcaactggtc taatttgatg gtctgacctt
     5881 catacaatct ttcaggctgc cgaaatacat aggaacgtcg agcacgctcg aaccgttctt
     5941 gtttgcgctg gatctggcgt acaaacgcct gctgcaatct agcttgccgt tcttgaatgc
     6001 gcatcaactc ttctgacaaa acaggaacag ctaattctgc agctgctgtt ggagtggcag
     6061 cccgaacatc tgctactaaa tcagcgatcg tggtatctgt ttcatgtcct acagatgaaa
     6121 tcaccggcgt attagcttga taaatcgccc gagcaacccg ctcttcatta aacggccata
     6181 aatcctcaat cgaaccaccc ccacgaccga tgatcattgt atcgaaattg cccatctctt
     6241 ctactctctg gatattcctg acaacattgt ccgctgcttg gtttccttgt acaactgttg
     6301 ggaacaaaac caattggaca attggatatc ttcttttcac cgtcgtgatg atatcgcgga
     6361 taaccgctcc gcttggactg gtaaggaccg cgattcgctt tgggaaacgt ggcagcattt
     6421 ttttaggccc ttgaaaaagg ccttccttca tcagcttttc ttttcgttct tccaattctt
     6481 gatataaagc acctattcca tcaggctcca tgtgctctac atagatttga taagaacctc
     6541 ccgcttcata taatgaaata cgcccaacga caaggacttt cattccttct tttggctgga
     6601 atcgtaattt ttggaatgct cctttgaaca taatagcaga tatttttgca tggtcatctt
     6661 ttaagctgaa atattgatgg gcattggcac gtaaacggaa attcgatatt tcgcctgtta
     6721 aatatacgcg ttctaaataa ggatcggcat caaattttct tttcaaatac ttcgttaatg
     6781 tagtgacagt taaatattct tgtgccatct attcacctgc tccaaaagct tcggcagatt
     6841 ttacggtttg atacatcagc atcgtgatag tcattggccc tacacccttt ggcacaggag
     6901 tgatccagcc tgcaataggc tccacttcat caaatttcac atcaccgatc agcttaccgt
     6961 tagcatcacg attcatccct acatctatca ctacagcacc tggttttacg aattcttttg
     7021 tgacaaaatg tccacgtcct attgctacga ctaaaatatc tgcttcttgt gccagttcag
     7081 ccagattttc ggttttagaa tgagcaacag taacagttgc atctgccatc aagagtagct
     7141 gagccatcgg tttaccgacg atattgcttc gtccgataac tactgctcgt ttacccgccg
     7201 gatcgataga ataagcttca aacattttca tgatgccgta tggagtacaa ggaatcttgt
     7261 ccggtttacc cgcaaggagt ctgcctaaat tcatcggatg aaaaccgtct acgtcttttt
     7321 cagggcggat agctaacagg atcttttctt catctatatg ctttggcaat ggtaattgga
     7381 ccaatatacc atgaaaacta ggatcttgat tgtatttttc aatttctgca agtagttcgt
     7441 cttcagaaat cgtctcagga taacgctcta ccaatgaatg gattccgatt tcttttgcta
     7501 cacgttcttt atttcttaca tagatttggc tggctggatt ttcccctacc aataaaacga
     7561 ctaatccggg atggatacca ttatctttca atttttggat tttttcagca atctctgcct
     7621 gcattttttc agctaattct tttccgttaa tcaattctgc catctaacca ctcccagata
     7681 ttttttgtgt tttcattcta ccatagaatt ttcccgattt ataacaggtt tatgatgatt
     7741 catagaaaaa agctaggact tacgccccag cttctaattc tttcaataca ttggataaaa
     7801 caccgttgac gaatttacgt gaacggtcat cactaaatgt ttttgccaac tcgattgctt
     7861 catttaaagc aacagccgct ggtacatctg tcacataagt catttcaaaa atcgcgatac
     7921 gcaagatcac caaatccatt tttgagatcc gattgatgtg ccaattgttt tttaaatgtt
     7981 tttcaatcac tttatccaat tcatcttttt ttgcacagac accgccaact agcgtatcaa
     8041 gataaacagg aacaaactct gattcatctt cgttgatcat gtcgcggtgg tctaattcga
     8101 ttgcgttgaa aatcgcatct tcttttgtta ggtctgcatt gaagtctaat ggaaataaag
     8161 cctgcaatgc catttcccga atctcgtgtc ttgataattc tttattcatt ctcttcctct
     8221 tcgtcttcaa aaagttcttc gattgttggt tgttctaatt tttcaggaac aacagctact
     8281 acatggatat tgacttcaga taaatcgata tccgtcatga aaaggacttg ctggctgacc
     8341 cgttcctgca tttccattgc tactttagga actgaaacac catattctag ataacagtaa
     8401 agatctactt tcaagccatc ttcctcgttt gctaagtaaa caccttttcc gtgagctgca
     8461 cgtcctaata attcagtgac attgctggca aaagttccgc gcattccgta tacgccttct
     8521 actttagaag ctgcaatccc gataattact tcgatgactt caggagcgat cacgatctct
     8581 cctaaatctt ggtttgcgtt taaaatcaaa tttttttcat ctgccattta tttattcctc
     8641 cctttgccta agcacgagat acatccgaat tctctatgat gcggcatagt gatcacgtgg
     8701 taagaaaact gccgccgatc agctgcaaac tgctatgaat tggagaaaac acccaacttt
     8761 attatctctt acagcttaaa agttcttcta tagtgtatca ttaaatcgag tgattttcta
     8821 taacaaagtc tgtgaacact gatttttacg cttacttctt ctattgatga aactataaga
     8881 tgatcaattc ttttggtgaa cgagtcaaca cacgatttcc atcttctgtg atcaacaagt
     8941 catcctcaat acgtactcca ccgattcctg gtaagtagat ccctggttca tcagtgatca
     9001 catttcctgg tacaaattgc ttttctgcac gagaagaaac gtttggtcct tcgtggatct
     9061 ctaaaccgat cccatgtcct gtagaatgtc caaatgcttc cccgtaacca tgttttgaaa
     9121 tataatctct agcaactgca tctaattgga caccagtcat tcctggtttt gcagcatcaa
     9181 taactgctaa ttgagcatct aagactattt gataaatttc ttttaatttt tccccaggat
     9241 caccaatagc gaatgtacgt gtcatatcgg aaacgtaccc ttcgtaatag caaccaaagt
     9301 ccaatgtgat caagtcacct tgttcgatga tttttttgct tgctacacca tgcggcatag
     9361 ccgaacgcaa tccgctagca acgatcgtat caaaagaaac acctgaagcg ccaagagaac
     9421 gcatataaaa gtctagttga tttgcgactt caatctcagt catgcccggt tggatcattt
     9481 tcaggatgtg atcatatgcc atatccgcaa tatgacatgc tttttcgata atagcgattt
     9541 cttcttcatc tttcacttct ctgagttctt cgatcatctg tgaaacaggt actaattccg
     9601 catcgatgat ttcttctaaa accgaatatt ctaaaaatga gactgttgtt tcctcaaaag
     9661 ctaatgagtg cagttcttct ttgcgtacta agtttgctac ctcatcaaaa ataggtccga
     9721 catttttaat aatttcaaac ccttgagcct gcgctgccgc ctgctcggta taacgaaaat
     9781 cagtaataaa aaatgctttt tccaaagtaa taacagctaa tcctgtcgta cctgtaaagt
     9841 tcgttaagta acgaagattg tacggactgg taaccaaaaa tgaatccaaa ttttcttctt
     9901 gcattttttt gcgcaatttt tctacacgta gcatcataat gaaactccct ctttctttgc
     9961 acgctttttt ccattatagc aaacttttct tcgtacacga cagttaaata aaaatttccg
    10021 gaaaacttct ttttatttta aataaataaa gccaaaaagg agtcacagtc tcgctgcaac
    10081 tccttttagt ttactatttt gtaaaaactt atgatcgttt tccaaagaaa aatgagacaa
    10141 ctgctaccaa aatcactgcg cctaaaatag atggaataag tgccatacct gcagcttttg
    10201 gtccccattc gccgaaaatc gcttgtccaa tacttgaacc gactagtcca gcaatgatat
    10261 tcgttacaca gcccatcgaa tttcctttac tggtgatcgc acctgcaatt gctccgatca
    10321 cggctccaac aatcaatgtc cataaccaac tcataatata acctccttct ttgctaatca
    10381 attttagcat atgggattta aaggggacaa ataattggtt ttgctatttc atattttctt
    10441 ctgcaaagta aaaaaataaa gaaaacctcc tcttagaagt tttctttatt ttttacttgc
    10501 gtttaaacat actacgaata ccacgtttca ctacatcaaa gaaactcaat gattgtacca
    10561 ttctatcttc atctacatat tctctgatcg cacgaagcac ttttttaggt tctttggaag
    10621 aaaatgtgta tgtaccattt tttttcgttt gaatggcata tcgaggaatc cattttccct
    10681 ttagcatgac tgatgctagt acataatcca cttcttccca tggaatttgg atatatttgc
    10741 gtctatctcg cgcatggtaa aactcaaaac cattatcacc tatcatgatt tgcccgtaat
    10801 ctgtcagacc cgtaaacgaa gtagcatcaa ttaccaagtc tacttttgta ttgattgatt
    10861 gaaccatttt ctccctccag atatttgttt atgaacagta tactatataa aaaaagctcg
    10921 ggacaacgcc cgagctatat ttttatggat tataaaagtc cgatgacgtg tccaacaaca
    10981 ccgactacga ataaaccaag gatgataacg attggagaaa cctttttctt aagcaaccac
    11041 atacataaca atgttaatcc taatgcagct aaaccaggaa tcaattggtc taagttgtct
    11101 tgtaatgttg ttaccttttc aggagaaagg gctaagccat tattaacttg ttcaaatgca
    11161 ctcttgattc cttcaccacc tgaaggcaat ttatcccatt cgatgtaagc acctttatct
    11221 aattttacag ttgatacaac tggtaagaat ttgattgata cccatctttg tacaagggca
    11281 gctaatacga acatccctaa aattgaagca ccttttgtta cgtcttgtaa caatccgcca
    11341 gaaaggtcat cagtaatttt agaaccagct ttgtagccaa attcttgtgt ataccacatg
    11401 aatgcccaac gaataatgtt ccaacctaag aagaagatga ttggtccaag gatatttcct
    11461 cccatagcta atgaagcacc tagtgcacct agcattggac gtactgtaaa ccagaacact
    11521 gggtcaccaa caccagctaa aggacccatc atacctactt taaccccttg aatcgctaca
    11581 tcatcaacag gcgcgccatt cgcacgttct tcttccagag ccaatgtaac ccctaaaatt
    11641 ggagaagcaa tgtatgggtg tgtattaaag aattccaagt gacgttttaa tgctgcagaa
    11701 cgttcttctt ttgttttgta taattttttg attgctggaa tcatagaaaa tgcccagcca
    11761 ccgttttgca tacgttcgta gttccatgaa ccttgaatga aggtagagcg ccacgcaaca
    11821 gctaaacgat ctttttttga taatttgatt ttttcttctg ccattttatt ttcctccttc
    11881 aagattaata gtcattcaaa atgtcgccta aagggtcacc agagttgctt ccgcctccat
    11941 tatttgatga accacccatt ttagaaaggt tcaagtaaat aagagctaat gcaacaccta
    12001 aagcacctag agcaatcaat gttaattgag aaattgcagc tacaacgaaa ccaataacga
    12061 agaatggcca tacttctttt gtagccatca tgttgattac caaagcgtaa cctacagcta
    12121 cgaccattcc cccaccgata gccataccat ctgttaacca tgctggcatt gattcaagga
    12181 atgattgcac agtttgtgct gggataaata gcaacgctgc tgctgggacc gcgatacgaa
    12241 ccccttgtaa acatacagca atgatgtgcc acatttcaac cttacggatg tttccttctt
    12301 cagcagccgc atccatcaaa tgaacgattg gaacagcgat tgtacgtacg atcattgtta
    12361 agaacaaccc tgcaacagcc aaaggaacgg caatcgcgat cgcagaagga acacctttaa
    12421 caccttgtcc acctaaaact aaaataattg ctgatgctac tgatgctaat gcagcatctg
    12481 gtgctacagc ggcaccgata tttgcccaac ctaaagcgat catctgaagc gtaccgccaa
    12541 gtacgatacc agcttcgagg ttaccggtaa ctaaaccgat caatgtacat gccactagtg
    12601 gttgatgaaa ttgaaattca tcaagaatac cttccatacc agctagaaag gcaactaata
    12661 cgactaaaat tattgaaata atagacatga taagcctcct ataaatttta atattattta
    12721 ttacgctttt gctaattcag cttttgcttt tttcaaaata tcatccatgt tatcgcgtga
    12781 atcattaggt actttacgta catcgaattt cacacctttt tgttcaagct tctcaaacgc
    12841 ttcaacgtct tcttgtccca tagacaatac tttacttaca acgactttac cgaccgaatg
    12901 cgccattgac ccaacattta attctttgat gtctacgcca ccttcaacag ctgtcaatac
    12961 gtcttcagga ttttcaaata aaagcaatgc tttggtattt ccaaaacgag ggtcttttgc
    13021 tacttcgatc attttgctaa taggaataac atttgctttt acacctggag gagcagcttg
    13081 ttcgattagt ctcttacgca gatcatcctt tgatactgca tctgaaacaa cgataatacg
    13141 gtttggttgc actgcttttg tccaagctgt cgcaacttgt ccatgtaaca gacgtgagtc
    13201 aacacgtgct aatacatatt tgatttttcc gtctccgacc acagttcctt ctggaagtgc
    13261 tcctttaggt tgtgcgtctt caactgctgc tttcggtgct tctgccggtt ctaattcttc
    13321 cggttttact tttacaccgt cttttgctgt ttcaatgatg tgtgctgcaa tttcatgagc
    13381 agattccatt gaaaagcgtg atgcgtaggc ttcaatcaac attggtaaat tcaaaccgct
    13441 gacgattgcc catttatctt tgtgctcttc gaataatgtg tttgattgat tgaacggtgt
    13501 tccgccccat aaatcaacta agaataacac ttcgtcttga ttatcaaaag aagcaaccgc
    13561 ttcttccaat ttggctctca agtcatctgg accttcactt ggtttcaaaa tcacggcttt
    13621 gacgttttct tgttcgccaa agatcattga accggattgt aagattcctt cagcaaattg
    13681 tccatgactt gctaggataa tccctaccat atttatacct cctatttact tttgttgtaa
    13741 caaattcaaa aatattaata attgctaacc attacaacag aattatttga ccttttccaa
    13801 caaccattct tgctacacct ttttgatttt cgctagtagt tccatcaaaa ggattttcct
    13861 gtcagcaggt acttttcgaa tctctaactc cacttcttga ctgttcaagt aacgaaagga
    13921 atcaatatct tgttgattta ctgaaacaaa attggtaatc attgatttgc cttcagtaaa
    13981 actcataccg ccgatattca acgacttcaa ctgtacaccg cagcggacaa ccgcttcaac
    14041 atcttgggga ttggtgaata aaagcatcac ttttgctcgt gataataatc cactatgata
    14101 gatttgaatc attttctgca cggttattac atttgctgtg atccccggcg gtgcagcttc
    14161 ttttagtaga aactttctca atttatcttg agcgactaca tcgctgatca caatgatacg
    14221 ttcaacacct gtttgcttcg accaaactgt tgcgacttgc ccgtggatta aacgatcatc
    14281 tatacgtgcc aatcgaatat ccatctgcaa aactccttct tcctcactac ttcacagaaa
    14341 cccctatcat ttctgtgtga acaaccacct cctagcacac actactataa gcaaaaagcg
    14401 tgccaacttt tttacgatac actaattgaa tttaaaggat tcttaaccaa tacacaaaaa
    14461 cgatacacta taaaagtgta tcgtttttgt gtattcatgg cacagtgtat tcttttttat
    14521 ctaactgatt atggactaga cgtagcagat aatacatttc ttctatcggc acttttactt
    14581 ttatctgatc ttccaacaga acaactgagg ctgccaattg gctataaaga ggatcattcg
    14641 ttgtatttgt caattcgttt tcttccacag tcaatggttc attccgaata atacgttcga
    14701 tcattcctgc tgtgtgcatc actaggttga ttttgaatcc atattcttca tcgccgattc
    14761 ccgtcgtctg agagatttgt gtagaaaatt gccacattgg atcgattaac ttactaccat
    14821 taataaagat gaagttatca gagataaact caacacacgt atttttagct gactcttcat
    14881 ctagggagat gaattcagtt tcctccaatt ctgattccat cagcaactga tccaaaatga
    14941 cttcgatatt ttgatcaata aaacgttcta aaggaataaa tggggcttgg atattcaaat
    15001 cagtaatccc agtagtagct atgatctgat attctttttg gataactggc agttcttctt
    15061 tcacttccac aatcgacaaa gctagaactt ctaattctgt ttcttgacgc ttgtttaccg
    15121 ctcgttcgat caattctttg attctttgtg ctgtaccttc gccagatgcg cagatagcca
    15181 aaacagcttt tttcttatga ataggttttg cttcctgaac agattctaca gaagatattt
    15241 ctgcgtaacc tcgaaaattt ttcaatgatt catgcaacat atctagatct gtccctaata
    15301 ctgagacctt tcgtacagtt tccaaaacta gagaagttgt taccatatca acagtacgta
    15361 ctggaattcc agtgtctctt tgaatttgcg aattaaacga cgcaagggac cccatatcta
    15421 ctaagagtat gaccccgctt ccttcatcta cttcttgtac attgcgttca attcggtcca
    15481 atgctgtttt cggatccata tccaaaggca tgtctactgc tcgtacgttc tctgcatcta
    15541 acaactgttg gacaacttgg accatactgc ttgctgtgct gtttccgtga gctgcaacga
    15601 ctacaccgat tctgccgctt gcttgatttg cccgtaacga gacgagtaga acggtcaagt
    15661 aatagtcttc actttctgga atctccactt gaaaatacgt ttcgatataa ctacgcactt
    15721 ctttcgcaac agcatattca tctggaaaat caaaagccat ctcacggatg ttatcattcg
    15781 tatgccgttc ttctcctaag ttgatttttt tcaaaaaaga actgatatgc aagctcatag
    15841 cataaataaa gttttgctga aactcatatg gtaaagatct tttgaccatt acataaatct
    15901 gattggtcac ttctatgatt ttcggatcta caaattctgc taatttgctg tctgcattaa
    15961 aagaaaaacc atggtcttta tagaaagact tcagatggat gttgatatct gtagaaatga
    16021 aatgattgat tgcttcttga tccaaaccat ccgacttcag taaagctgct ttatctccga
    16081 tgatatcata tagattataa ggtaactcat aggagtccga ttggattttc atgatcgtat
    16141 catttggtgc tacagttatc tttggctcca atattttaga aagctctgcc attgcactgc
    16201 ggttgcttgc taattgaatc aatccgctac gaatcccttc tgtcaaatca tcgattgtga
    16261 tcgagatttc gggtgattgc atatgattta gaaatcctcg tgcacatact aactggatat
    16321 ttgacttcaa ttgtcctata tttccatagg tcacactgcc gatcaatgct tttgctacat
    16381 cttctgtcaa agaaatcttt cgctgcgtgc ggtttgcttc gtgggcaatc atgattttta
    16441 ctaaatcgac tttctcattt gcaggacgtt tttcaaaagc cggaagtttg atattgattg
    16501 ggatacgccg aacaaaagtt tctaatagag aagaacctgg gtcttctgtg gtcgcaccta
    16561 cgatccgtac atttgcttca tgggatttcg ttgtttcccc aagacgactg taagttccat
    16621 gatccataaa gtaaaagatc atttcttgac cttcaggcgg caaccggtga atctcatcca
    16681 aaaacagcat accgccatcc gcttgatcga taatccccat tttttcttca tctgcgcctg
    16741 taaaagcacc ttttgcgtac ccaaataaat gactcatcaa aagctcaggg ttattggcat
    16801 aatcagcaca attgaaaaca atcagttccg tttcctcatc tattacatga tgcgcttttg
    16861 caaaatgata catcgcgtgt gcaaaataag tctttcctga tccggtaggt cctgtaatca
    16921 aacaatttaa tcctcttggc ggatacaaaa tcgcagcttt tgcctgttcc actgaatttt
    16981 tcatgctgcc gttagctcca atgatttttg caaagatatc cttagtatct atttggagtg
    17041 tatttttttc ttcagagacc ggcgtttctt tgtaactgac tacatgtttt gtaactggct
    17101 ttacgtgcgt aacgagtgtt tgatatatgt aacgtactgg tcgtccgtct gtctttatca
    17161 gttttccttc gcgcactagc tgattcagat ctttactgct atttgttcgc tgaatcccta
    17221 acacttcagc aatttcctgt gttgttactc cttgatcata ttctgccttt gttaaatgag
    17281 cggttttttc tttgacatac atataaattc ggtcaatgcg ttttgccaat ggtatcgctc
    17341 cttttctata gactattgta cttaggcttc aaaacataat actatgtatt tatttctgtt
    17401 tcgtcaacta tttacctgat tttgttttgt tgataacacg cggatagata gaaatgttca
    17461 atttaaaaga acgaaaaatc ccgatacgat ttttataatg gaaacaagaa aaagaacact
    17521 gtttaccata taattatttc actgatagca aacaatatag cttccatcaa ttgaaaacga
    17581 tagttatcaa aacatcctga ttccaaattg gcaaaaaaat attaagtgta aaaagacaaa
    17641 gtaaacttcg tagcctccac tacaactttt atttttataa acaaaaaagg ctgtgacaaa
//
LOCUS       DANJJH010000060        17129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000060 DANJJH010000000
VERSION     DANJJH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..626)
                     /locus_tag="PWS53_001868"
     CDS             complement(<1..626)
                     /locus_tag="PWS53_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0872086.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="PWS53_001869"
     CDS             899..3370
                     /locus_tag="PWS53_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0872087.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="PWS53_001870"
     CDS             complement(3473..5209)
                     /locus_tag="PWS53_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL0872088.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="PWS53_001871"
     CDS             complement(5206..5910)
                     /locus_tag="PWS53_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0872089.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="PWS53_001872"
     CDS             6041..6544
                     /locus_tag="PWS53_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL0872090.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="PWS53_001873"
     CDS             6504..6842
                     /locus_tag="PWS53_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL0872091.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="PWS53_001874"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="PWS53_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL0872092.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="PWS53_001875"
     CDS             8393..8971
                     /locus_tag="PWS53_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL0872093.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="PWS53_001876"
     CDS             8975..9496
                     /locus_tag="PWS53_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0872094.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="PWS53_001877"
     CDS             complement(9575..10129)
                     /locus_tag="PWS53_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0872095.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="PWS53_001878"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="PWS53_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL0872096.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="PWS53_001879"
     CDS             10669..10920
                     /locus_tag="PWS53_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL0872097.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="PWS53_001880"
     CDS             11045..11836
                     /locus_tag="PWS53_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872098.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="PWS53_001881"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="PWS53_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL0872099.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="PWS53_001882"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="PWS53_001882"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL0872100.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="PWS53_001883"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="PWS53_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL0872101.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="PWS53_001884"
     CDS             complement(13893..15029)
                     /locus_tag="PWS53_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872102.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="PWS53_001885"
     CDS             complement(15145..16059)
                     /locus_tag="PWS53_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0872103.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="PWS53_001886"
     CDS             complement(16150..16578)
                     /locus_tag="PWS53_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0872104.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="PWS53_001887"
     CDS             complement(16583..16957)
                     /locus_tag="PWS53_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872105.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5510 a         3098 c         3361 g         5160 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcat
//
LOCUS       DANJJH010000061        16665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000061 DANJJH010000000
VERSION     DANJJH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(157..864)
                     /locus_tag="PWS53_001888"
     CDS             complement(157..864)
                     /locus_tag="PWS53_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL0872106.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="PWS53_001889"
     CDS             complement(943..1281)
                     /locus_tag="PWS53_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL0872107.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="PWS53_001890"
     CDS             complement(1392..2354)
                     /locus_tag="PWS53_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL0872108.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="PWS53_001891"
     CDS             complement(2369..3700)
                     /locus_tag="PWS53_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0872109.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="PWS53_001892"
     CDS             complement(3708..4388)
                     /locus_tag="PWS53_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0872110.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="PWS53_001893"
     CDS             complement(4607..5086)
                     /locus_tag="PWS53_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL0872111.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="PWS53_001894"
     CDS             complement(5213..6892)
                     /locus_tag="PWS53_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0872112.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="PWS53_001895"
     CDS             7440..8942
                     /locus_tag="PWS53_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872113.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="PWS53_001896"
     CDS             8955..9617
                     /locus_tag="PWS53_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872114.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="PWS53_001897"
     CDS             complement(9718..9921)
                     /locus_tag="PWS53_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0872115.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="PWS53_001898"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="PWS53_001898"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL0872116.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="PWS53_001899"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="PWS53_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0872117.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="PWS53_001900"
     CDS             complement(13885..14499)
                     /locus_tag="PWS53_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0872118.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="PWS53_001901"
     CDS             complement(15105..15482)
                     /locus_tag="PWS53_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872119.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="PWS53_001902"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="PWS53_001902"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0872120.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3592 c         2846 g         5398 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cactt
//
LOCUS       DANJJH010000062        16637 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000062 DANJJH010000000
VERSION     DANJJH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(29..727)
                     /locus_tag="PWS53_001903"
     CDS             complement(29..727)
                     /locus_tag="PWS53_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL0872121.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="PWS53_001904"
     CDS             complement(720..2072)
                     /locus_tag="PWS53_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL0872122.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="PWS53_001905"
     CDS             complement(2295..3695)
                     /locus_tag="PWS53_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL0872123.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="PWS53_001906"
     CDS             3856..4593
                     /locus_tag="PWS53_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL0872124.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="PWS53_001907"
     CDS             4718..5197
                     /locus_tag="PWS53_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL0872125.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="PWS53_001908"
     CDS             5194..5961
                     /locus_tag="PWS53_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872126.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="PWS53_001909"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="PWS53_001909"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0872127.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="PWS53_001910"
     CDS             complement(6864..7466)
                     /locus_tag="PWS53_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0872128.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="PWS53_001911"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="PWS53_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="PWS53_001912"
     CDS             8147..8731
                     /locus_tag="PWS53_001912"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0872129.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="PWS53_001913"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="PWS53_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL0872130.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="PWS53_001914"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="PWS53_001914"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL0872131.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="PWS53_001915"
     CDS             10632..10811
                     /locus_tag="PWS53_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872132.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="PWS53_001916"
     CDS             10833..11858
                     /locus_tag="PWS53_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL0872133.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="PWS53_001917"
     CDS             12033..13277
                     /locus_tag="PWS53_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL0872134.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="PWS53_001918"
     CDS             13430..14377
                     /locus_tag="PWS53_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0872135.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="PWS53_001919"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="PWS53_001919"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL0872136.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="PWS53_001920"
     CDS             complement(15279..15521)
                     /locus_tag="PWS53_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872137.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="PWS53_001921"
     tRNA            15642..15714
                     /locus_tag="PWS53_001921"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="PWS53_001922"
     CDS             complement(15833..16471)
                     /locus_tag="PWS53_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0872138.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5672 a         2959 c         3336 g         4670 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtg
//
LOCUS       DANJJH010000063        16492 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000063 DANJJH010000000
VERSION     DANJJH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            1..1452
                     /locus_tag="PWS53_001923"
     CDS             1..1452
                     /locus_tag="PWS53_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0872139.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="PWS53_001924"
     CDS             1801..2433
                     /locus_tag="PWS53_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL0872140.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="PWS53_001925"
     CDS             3021..4460
                     /locus_tag="PWS53_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0872141.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="PWS53_001926"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="PWS53_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="PWS53_001927"
     CDS             5222..5437
                     /locus_tag="PWS53_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872142.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="PWS53_001928"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="PWS53_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="PWS53_001929"
     CDS             complement(6097..7008)
                     /locus_tag="PWS53_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872143.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="PWS53_001930"
     CDS             complement(7027..8031)
                     /locus_tag="PWS53_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0872144.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="PWS53_001931"
     CDS             complement(8028..10151)
                     /locus_tag="PWS53_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0872145.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="PWS53_001932"
     CDS             complement(10156..12603)
                     /locus_tag="PWS53_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0872146.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="PWS53_001933"
     CDS             complement(12590..12979)
                     /locus_tag="PWS53_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872147.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="PWS53_001934"
     CDS             complement(13039..13542)
                     /locus_tag="PWS53_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0872148.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13779)
                     /locus_tag="PWS53_001935"
     CDS             complement(13555..13779)
                     /locus_tag="PWS53_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872149.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="PWS53_001936"
     CDS             complement(13883..15793)
                     /gene="ltrA"
                     /locus_tag="PWS53_001936"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL0872150.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         4913 a         3271 c         2939 g         5369 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    13741 taaaaaccat tgatatatgg ataggtaagt tcctgatatt ataaatatct caaacttttc
    13801 cccccattca cacggagcgt gcgactttca ccgcactccg cgttccatcg tgtttatatt
    13861 gaaaagtatt tatgtagtag acctatatca agtttaattc ttgattgcca gctaatactc
    13921 tatatttatt tagtcttatg agctggactt tatttagctt caatagagct atatattttt
    13981 gtattgtttc ttctgtttta gcgtgtatta gacggtgtac cgcctctttt accaaaataa
    14041 gatttttata atcgtcattt ccaccagctc cagttggtac aatatgatga caatgtattt
    14101 cattagcttg ttgaaattct tccagagtta tagcacattt tccatgttga gcagaaaata
    14161 gcgaaattcg attgtctgcg tattctattg tgcgattttc caaagataat ttcattaact
    14221 ggtacatcag taatttatca acacttagat tggtgtggat aaattttctt ccctctagag
    14281 tgtagcaatt cgctaaatac acctttgcca ttggttttct gtgttggata tatccaacag
    14341 ggaaaatagg ttcgccctcg ccagcaacat accgaagcat tttagattta ccatatcttg
    14401 ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa tctgcccctt ttgtttgtac
    14461 ctaatctgtt agtgaagata gccattacag ctctattcag tttcatacaa tccagattta
    14521 cattcgttgc aatctgataa taattttgca tacctaatac cattgaatta taaagtcttg
    14581 tttcttctaa ttccgttttc ccttttgagg gagaagcaat tttctttgcc tgtgatacca
    14641 gcttgtcttt ttggtttttg aattgtttat cacttatatg tgatttaaca actaacttct
    14701 ttcttctcgg tatcatttta atcttgaaac caagaaagtc ggagtatctt tttctaacat
    14761 ttacaattct tgttttttct tgtgatactt ccaattttaa tcgttgttca atccattgag
    14821 tgattgctat tttggctttt tcagcacttt ccttgtagcg gcagaaaact ctaaaatcat
    14881 ctgcataacg aaccatatac atttccttta actttgtttt cttcatttct ttgtaagcgt
    14941 tgcttttaca tgggctaccg cttttattga tatgaaccac atagtttttt gtaacaggat
    15001 tttcctgcca ttggctttct acccaatggt ctaactcatt aagaacaatg tttgctagta
    15061 gtggggatat tattccccct tgcggagttc ctttctctgg atatgttatt gtaccgttag
    15121 tcattttgat tggagctttt aatatccttt ttaaaatgaa tatcaagtgc ttgtcccgta
    15181 ttcccatagc ccatatctgt tttatcagct tggaatggtt cacgttgtca aagaaaccct
    15241 taatatcaaa ttcaatcaca taatgtagct ggctgatttg taggcggtta taggttgctt
    15301 ttattgcatt ttcaactgac ctattaggtc taaaaccata gctgttttca ctaaatttag
    15361 cttcgcagat tggttctaat acttgtttta tacattgttg tataagcctg tcccacatac
    15421 aaggaatacc tagaggtctg gttttaccat ttggttttgg tatttctttt cgcctcactg
    15481 gcttaggtcg atacccatgt ggactgcctg caacaatgta tcttactttt ttgacaactg
    15541 tttctgctgg caatttgcca atatctttaa ttattgtacc gtctgttcct gctgttttac
    15601 tgcctgcatt tgctttgata tttcgatatg ccagtaatat attttctctc gataggataa
    15661 tatccataag atgagtgaaa atatctccat tcttactttt ggcatataaa tcatcaaagg
    15721 tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc tacacacaat gtttcatttt
    15781 tatttttagg cataaggctt cactccttta cttgaaattg agtgagcttt tttatcttac
    15841 tcgtaatcct tattgaaaca acataatact tttctaatat atacacgact tgagggcatt
    15901 cctccattct cattacaaga atttcttagg ttcgccctct gcttttcagc attagttcaa
    15961 ctgcttattg ttcttcgtca gtttattctc ttgatgagaa cccaatacct ttcctcgttc
    16021 cgataatctt atctatacat atatatcctt aggtcactag ctataagcct gctggcttga
    16081 taccgcctgt aacgttatat ggcatttcat aacctatatt cttactttgc cacaccagct
    16141 acgatttctc gtacctttgc atttctacaa agttcaaatt tagacccgta cattcgctgt
    16201 ttcgtcagtt ccttatagaa cattctaacc atagatattt tatagacccc cgacctgttg
    16261 gatacgctac ccacctctgg atagttttcg atagaagata tatgttcatc aactattacg
    16321 gtatctttca gccgtcttta ccgagcttca aacattctga gtttcctcat acaatgcttg
    16381 tcggaggctc agggcaggcg tttcagagcg ttacttcatc attccacctg tcagattatc
    16441 agttctccta ataaccaaac tttttcgttc ttttattagt gccttagctt tt
//
LOCUS       DANJJH010000064        15955 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000064 DANJJH010000000
VERSION     DANJJH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(391..813)
                     /locus_tag="PWS53_001937"
     CDS             complement(391..813)
                     /locus_tag="PWS53_001937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL0872151.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(827..2233)
                     /gene="atpD"
                     /locus_tag="PWS53_001938"
     CDS             complement(827..2233)
                     /gene="atpD"
                     /locus_tag="PWS53_001938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL0872152.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2256..3158)
                     /locus_tag="PWS53_001939"
     CDS             complement(2256..3158)
                     /locus_tag="PWS53_001939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL0872153.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3172..4728)
                     /locus_tag="PWS53_001940"
     CDS             complement(3172..4728)
                     /locus_tag="PWS53_001940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL0872154.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4751..5293)
                     /locus_tag="PWS53_001941"
     CDS             complement(4751..5293)
                     /locus_tag="PWS53_001941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL0872155.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5280..5804)
                     /gene="atpF"
                     /locus_tag="PWS53_001942"
     CDS             complement(5280..5804)
                     /gene="atpF"
                     /locus_tag="PWS53_001942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL0872156.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5895..6110)
                     /gene="atpE"
                     /locus_tag="PWS53_001943"
     CDS             complement(5895..6110)
                     /gene="atpE"
                     /locus_tag="PWS53_001943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL0872157.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6163..6882)
                     /gene="atpB"
                     /locus_tag="PWS53_001944"
     CDS             complement(6163..6882)
                     /gene="atpB"
                     /locus_tag="PWS53_001944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL0872158.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7184..10027)
                     /gene="rnr"
                     /locus_tag="PWS53_001945"
                     /pseudo
     CDS             complement(7184..10027)
                     /gene="rnr"
                     /locus_tag="PWS53_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10030..10785)
                     /locus_tag="PWS53_001946"
     CDS             complement(10030..10785)
                     /locus_tag="PWS53_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0872159.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10888..11124)
                     /gene="secG"
                     /locus_tag="PWS53_001947"
     CDS             complement(10888..11124)
                     /gene="secG"
                     /locus_tag="PWS53_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL0872160.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11242..11520)
                     /locus_tag="PWS53_001948"
     CDS             complement(11242..11520)
                     /locus_tag="PWS53_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0872161.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11633..12601)
                     /locus_tag="PWS53_001949"
     CDS             complement(11633..12601)
                     /locus_tag="PWS53_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0872162.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12816..12860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12884..13858
                     /locus_tag="PWS53_001950"
     CDS             12884..13858
                     /locus_tag="PWS53_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDL0872163.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13863..14438
                     /locus_tag="PWS53_001951"
     CDS             13863..14438
                     /locus_tag="PWS53_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0872164.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14505..15644)
                     /locus_tag="PWS53_001952"
     CDS             complement(14505..15644)
                     /locus_tag="PWS53_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL0872165.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4360 a         3443 c         2856 g         5296 t
ORIGIN      
        1 aaatctgagc cagaatgtgg ttcattctga ctcagatttg attttgcaca actcatcagt
       61 ccttattgac aaagagccca aaagtcttgt cgcagtccct tattccaaat aaacggtgtt
      121 caatcatctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttcca
      181 tttgtttctc taatactcag gacaaaacgc tgatttcaca acctccgata tacggtgttc
      241 cagaagtagc ttcttcgaaa aataagctga gatttcacaa aaatttgaga agcaattttc
      301 gtaaattcct tcttatgtct tgaagctgac cacttctgtc ataacctctt tttactgttc
      361 ttaaacacgg ataaacatcc gcatctcatt ttatgagtgc ttagatactt tgatacgatt
      421 gattgctcgg tgcaacgcaa cagttgcccg tttcaattcg ttcgtatctt gttttgcttt
      481 tgcttcttcg atttggcgtt ctgcgcgttg ttttgcacgt tccgcacgag atacatcgat
      541 atcacgttca cgttccgcac tatcagcaac gattgaaaca acattatcac gaacttccat
      601 gattccgcca ttaacagcga tccaatcgac gtgggtatct gaatctgttc gttttacacg
      661 aacttcatca atcgtcaatg gaacgatgat tggtgcatgt ttaggcaaaa tcccaagctc
      721 accagctgtc gtacgtgcaa cgacgatagc tgcatggtga tcatagacca aaccggcagg
      781 agttaccaca tttactgtta aatattgatc catggggcac ccctttctag tagcctagtg
      841 ttttcgcttt ttctacgacg tcttcgattc ggccaacact tcggaaagct tcttcaggaa
      901 gatcatcgta tttgccttcc aagatttcgc ggaatccttt cactgtttct tctacaggga
      961 catatgaacc tggaagacca gtaaattgtt cagccacgtt aaagttttgt gacaagaaga
     1021 attggactct gcgggcacga gaaactaaca ctttttcttg atcggataat tcatccatac
     1081 ctaagatagc aatgatatct tgtaattcac gatatcgttg caatacgtgt tggacttctg
     1141 ttgctacttt ataatgttct tctcccacga tttcaggtgc taaagcactt gaagaagaag
     1201 ccaatggatc taccgctgga taaatccctt gttctgttaa tttacgttcc aagttggttg
     1261 tcgcatccaa atgggcgaat gctgttgccg gcgccgggtc agtgtagtca tcggctggca
     1321 catagattgc ttggatcgat gtgatcgaac cttttttcgt agatgtgata cgttcttgca
     1381 attgacccat ttctgttgcc aatgttggtt gataaccaac cgcagaaggc atccgtccta
     1441 acaaggctga tacttcagat ccagcttgag tgaaacggaa aatgttgtcg ataaacaaca
     1501 gtacatcttg accttctaca tcacggaaat attccgcaat cgtcaaacca gtcaaagcaa
     1561 cacgcatacg tgcacctggt ggttcgttca tttgtccgaa caccatggct gttttttcga
     1621 taactcctga atctttcatt tcataataca agtcattacc ttcacgagta cgttcaccta
     1681 caccggtaaa tacagaaata ccaccatgtt cttgggcgat attatggatc agttcttgga
     1741 tcagtacggt tttaccaaca ccggcaccac cgaatagtcc gactttccca ccttttaaat
     1801 atggggcaag caaatcgata actttgatcc ctgtttccaa aatttctgta cttgtactta
     1861 attcgtcaaa ggctggtgct tttttatgaa tttcgcttct ttccgcatct tctgggaaag
     1921 gtgtttctaa atcgatcgta tctcctagta cgttgaatac gcgacctaat gtttctttac
     1981 ctacaggaac agagattgct ttgcctgtat caatgacttc cattcctctt tgtaaaccat
     2041 ctgttgattc catcgcaatg gtccggatca ctccgtcccc taattctaac gctgcttcaa
     2101 gaacaacttt tgatttattt tcgtcatttt tataaacaac taatgcgttg ttgatatctg
     2161 gtaaggattg gtctaaagaa aattccacgt cgacaacggg accgattact tgaacaatct
     2221 tgcctgaact catctttttt cctccaatct caatactatt ctagtgccga agctccggca
     2281 acaatttcgg taatttcttg ggtaatagcc ccttgtcttg cacggttgta agaaaccgtc
     2341 agctcatcaa tgatattagc agcattatca gttgctgttt tcattgctgt cataccagca
     2401 gcatgttctg ctgttttggc gtcaacgatc gcaccgtaga tcaaactttc agcatactga
     2461 ggcaataact gagacaagat ttcttctttt gaaggttcaa aaatatattc ttgttcgaaa
     2521 gtagttgctt cttctggatc taagtctgag attggcagca ttttttctac acgaaactga
     2581 ctagttagcg aattgatatg atggttataa catacgtata attcatcgaa tacttcgttt
     2641 tggtacatag ttgttgccat atttacgatt ttgcggactt catcaaagct tggctgatca
     2701 gatagattgc gcagctcata tgctagattg ataccgcgtg ctttaaagaa gtctgcacca
     2761 gttccgccaa ttgcgatcat cacgtaatca tcgggtgatt ggtggtcttc ttctaaaata
     2821 gacatcgttt gtttcaatat cgagctgtta tatccaccga ctaaaccgcc atctgcagtg
     2881 atgacgatat aacccgtttt cttaacagga cggctgagca acatgctgtt gtaattgata
     2941 tcccctctag gattttctga ggcaatatca tttaactgag tagcagtcaa atgcgtaacg
     3001 atttctctta cttttgttgc ataaacctga aacttctgtg aagaagcttc agattttgtc
     3061 aacttagaag cagacaccat ctgcattgcc cgcgtgattt ggctcgtttt tttcgttgaa
     3121 gcgatacgtg tcttgatttc atttaatgaa gcacccattc atctcacctc actatgcatt
     3181 ttgaatggat ttcaatttat cttctgtaga ggaattattt ccttttgtag ctaagaacat
     3241 gtctttatat tcctgaatcg cactgttcaa cgcttcttct tctggaagat cttttgttgt
     3301 acggattgtt tcaaagatat ctgaatgatt ggtatccaga tattcgaata attcatgttc
     3361 gaagtctaaa atactgtcta cagggatact atctaagaat ccatgtgtca aagcatacaa
     3421 aatcactact tgtttttcaa cagctagcgg tgcatgcaat ttttgtttca agatttctac
     3481 tgtacggcgt ccacgattta gttttgcttg tgttgctgca tccaagtcag aaccgaattg
     3541 tgtaaatgct tctaattcac ggtaacttgc taagtctaga cgtaaagtcc cagcaacttt
     3601 tttcattgct ttgatttgcg cagaaccccc tacacgagat acagatagtc cggcatcaac
     3661 cgctggacga gtacctgcgt agaacaagtc gctttctaag aagatttgtc catcagtgat
     3721 cgaaataacg tttgttggga tataagcaga gatatctcca gcttgtgttt caacaaatgg
     3781 taaggcagtc attgaaccgc cacctaaatc atcacttaat tttgctgcac gttccaataa
     3841 gcgtgagtgt aagtagaaaa catcccctgg ataagcttca cgacctggtg gacggcgaag
     3901 cagcaaggaa agttcacgat aagcaacagc ttgttttgaa agatcatcaa aaatgatcaa
     3961 gacatgtttc ccgttgtaca tgaactcttc acccattgca gtaccagcat atggtgcgat
     4021 ataaagcaac ggcgctggtt gagacgcacc ggcattcaca acgattgtgt aatccattgc
     4081 gccatatttt ttcaatgttt caacttgtgt acgaactgta gaatcttttt gtccgattgc
     4141 tacatagata cagatcatat cttgaccttt ttggttgatg atcgtatcga tcgcaatgga
     4201 agttttccct gttttacggt caccgataac taattcacgt tggccgcgtc cgattggtac
     4261 aagagcgtcg attgctttta gacctgtttg cattggttcg ttaacagatt tacgttgcat
     4321 aacgcctggt gccattgctt ctactggacg agctttgtca gtaacgattt cacctagtcc
     4381 gtcaattggc tgacctagcg gattaactac ccgtccaatc aaggcctctc caactggaac
     4441 ttccatgatc tttcctgttc gttttacttt atctccttca cgaatggact cgaaatcgcc
     4501 aaggataatg atacctacat catttgattc taagttttgc gccattccgt atgagccatt
     4561 tgaaaattca agcaactcac cactcatcgc gttttctaaa ccatgtgcac gagcgatccc
     4621 gtcacctaca taagttacgg taccgatttc ttcgactgaa agcacgtttt gataattttc
     4681 aatttgttct ttaatcaagg cactaatttc ttctgctttg atggccattc gattcaccta
     4741 cctctttgta ctatctattt aattgattac gcatattttc taattgcgtg cgaatgcttc
     4801 cgtcgattac tcgatggtta gcttcaacaa tcgcgccacc aataatagaa gaatcaacga
     4861 tttgttttaa ttcgactgat tgatagttca ttgtttttgc aacattcttt tctaattgta
     4921 ttcgttgttc gtctgaaagt ggaactgctg ttgtgacact tcccaacagc aaaccttgat
     4981 gttcatcata tcgacgttca tattcttcga tcatgaacaa aagatcatcc attcggttat
     5041 acgcataaac aacttctaaa aagtttttaa cgattccatc atagccacta acaagcttgt
     5101 ccatgatttc tcgtttttca tgaggttcta gacgaacatc acttaacata ttacctaatc
     5161 caggtacttg ttcatagatt tgacgtaagc ttacgagatc ttgataaatt tcttctgctt
     5221 tatttgaatc gatcgccagt tcaaataaag ctttcccgta acgtcttcct actgtatatt
     5281 tatctagttt catttgaaga acctagacct tcgatatatt gattgattaa tgattcgtgc
     5341 atttctggag ataattcttt gttcaagatt ttttccgcga tttgaagaga tagttctgca
     5401 acatcgtctt ttacagagtt cagcgctgaa tcacgttcta cagtgatatc agcttgggct
     5461 ttacttttta gacgagctgc ttcttcttga gcatccttca aaatattttg gcggcttaat
     5521 tctccacttt ctttcgcatt tttgatgata tcagctgcat cagaacgaga ggctaacaat
     5581 tgttgttcgc gttcttgttc cattttcgct gagttgatgc gagattgttc tgcagaatcc
     5641 aaatcattgg cgatcttgtc ttcacgtttt ttcaaaatat cgctgattgg tccccaagca
     5701 aaatgcttca agagagccag tagtatcaaa aatgagccgc tgacaacgat gacatttcct
     5761 aacatcgcat tgccaacttc tgcaattgcc aattgattca gcataggcga gccttccttt
     5821 ctttgaagta cttctagttt tcatgtaaaa aagcagtaac aaaccaaatt gtttctgcct
     5881 taatactgat tcaattaaac accaaaaact agaatcaagg cgacaactac ccctaagata
     5941 ggaacagctt cgactagagc taccccgata aacattgttg tacggaattg accagacatt
     6001 tcaggttggc gagccattga ttcgattgtt ttagagataa cttgaccgtt accataaccg
     6061 gctccgattg ctgctcccat gattgcgatt gctgctgcga tataattcat aattgttttc
     6121 ctcctaaatt agataaatag tagtttgttt ttaaaacgaa tattattctt ccacttcaat
     6181 cttatggctc atataaacca tagataaagt aacgaagaca aatgcttgga tgcttccgat
     6241 aaacaaggag aatgcgatcc agaccatctc taatggaatt gctaacggca atgagaacca
     6301 gcctaagctt attaccattt ttgcaatcaa ggttagcaaa acttcccccg cataaatatt
     6361 cccataaaga cgtaaagcaa gtgttaataa gtttgtaaat tcttcaatca gtttcatcgg
     6421 aaacataaaa ctatatggtt tcaagaacga atttacgaaa tatcctttga agcctaactt
     6481 tttcacccca aataagtgag taagtaagat cattataaat gccaaagtta aagtaacaaa
     6541 cgggtctgct gtcgggcttt tccaaagtgt tgtttcgtta ggtaaaacga tttttgttac
     6601 taaaccgatg ttgtttgcaa caaaaacaaa taaaaacaat gtaaatgcca acaaatggaa
     6661 attgctcaat tcttttctag gcagattatc tgttacgatt cctcgactaa aatcaatgac
     6721 ccattctatc gcattttgtt tgcctgttgg tttcaactta agatttcttg taaaaaagta
     6781 agctaagaag aaaacaatca aacaggtcag taacaccatt atacaaactg taccatcaaa
     6841 ccaaattggt ccaatgtgga acgttagcga gcgttcatcc aatccatttc acctcttttc
     6901 cccgaaattc acataaaatc aattcatgtg cgtatctggt tttttccaat cggtttctaa
     6961 ccacacggta tcataccacc atgaaacaga aaattcaaaa cattttcatg aaaattagac
     7021 acttttttat ttcagtcaaa aacgaagcga atttttcttt atacacggtg ctcctgatct
     7081 aagaaaaaac ctatgataat catgttttat cagattaaat gctaacaaat tttatcatta
     7141 agtaaaagca aaagcaccga atatgcgaaa aaggataatg aaattatcta gagaaatttt
     7201 ttaatgttcg atcaatttgt cgatcgatat cttttcgttt caaatcttcc cgtttatcat
     7261 aagatttttt ccctttcgcc agaccaatca ataccttggc atagccgtta cgaatataga
     7321 cttttaaagg aacaacagtg attccagtat ttttcaattc attttcaagc cggatgattt
     7381 gttttttgtg taataataac tttctcgtgc gcaacggatc atgattaaaa atatttcctt
     7441 gttcataagg actgatatga acattatgca agaaagcttc tccgttgcgg acgcgaataa
     7501 atccatcttt tagattgatc cggctgtttc gtatcgactt gatctcggtt ccttgcaaga
     7561 ccatccctgc ttccatcgtg tcgatgatcg aataatcatg gcgagctttc ttgttttgtg
     7621 caattaattt tccctcgcct tttggcatag ctgtttcctc caactcattt tattttttct
     7681 ttttcttctt tttaacacct ttgtaaaatg gcttcttccc ttttttctta ccgaacgttt
     7741 gcttactatc gctatatcgt ttgttcttct tgccatttct ttctgggcgt tttcctgctt
     7801 gttttccttc gcgttttcct gattttccat ttcgtgtttt tggccgttcc aacgctgtaa
     7861 cctcttctgc ggaaacaagt tcaaaatcta ctgctcgttc atctggatca gctttgacta
     7921 cttttactcg cactttctgt ccgattttaa aagtcagacc agttctttca cctacaagag
     7981 ctaaatgatt ttcaacaaaa tggaagtagt cctgtttcaa ttcattgata tggatcaatc
     8041 cctctactgt attcggcagt tcgataaaca ggccgaattt cacaacagag ctgatgatcc
     8101 catcgaattc ttcccccact ttgtccatca tgaattctgc tttcttcatg gaatctactt
     8161 cacgttctgt ttctactgca cgacgttcca tcttagaact atgatctgca atttcaggaa
     8221 gttccgtagc ccacgaactt tgattgcttt cacttttatc ttcactgtaa gaacggatca
     8281 gacgatggac gattagatcg ggatagcgtc gaataggaga tgtaaagtgt gtgtagtaat
     8341 ctgccgctaa gccataatgc ccaaaattgt cttctgaata gcgtgcttgt tgcatgcttc
     8401 ttagcaacat agtattgata actgcagctt ctggtttgtc ttctactcct tcgatcacat
     8461 gctgtaaatc ttttggtgtg atcgttcctt tcgttcctcg gaccagaatg cccaaagctg
     8521 aagcaaaatc aaagaagcgc tgcatttttt cttcttttgg ctgttcatgg attcggtaaa
     8581 taaacggcag atttaattta ttataatgtt cagctaccgt ttcattggcc gcaagcatga
     8641 aagattcgat caagcgttca ccaattcctc tgcttcttag ctggatatca tttgggtgtc
     8701 cttcagcatc aacaaggatt tttgcttcgc gatcctcaaa agaaatcgcg ccacgtttga
     8761 ttcgcatcgt ttcaaggatt cgatgcaatt cacccatttc ttggaacata ggtacgagct
     8821 gagcatagcg ttgcatcgtt tcttcgtctt gatcttctaa gatttgattg acagctgtgt
     8881 aggtcatccg ttctgttgtt tggatcacac ttgggaaaat atcatgagag actacttgtc
     8941 catttggatc aatctccatt tcgcaactca tggtcagcct tggaacatgg ggattcagtg
     9001 aacagatacc attcgataaa cgttgcggga tcatcggaat cactcgatca gtcaaatata
     9061 cacttgtccc tctttcatat gcttctctgt ccaattgact gccttgtgtc acatagtaag
     9121 aaacatctgc aatgtgtact ccaagaaaat aattaccgtt atctaatttt ttaaccgtaa
     9181 ctgcatcatc tagatctttt gcatcttctc catcaatcgt aacaatgagc tgatttctta
     9241 aatctctacg tccagttata tccttttcgc tgatttgatc tggaacttca tctgcttcag
     9301 acaatacatc atcaggaaat ttcgtaggga taccattagc cacgacaatt gagaggatat
     9361 ccatgccagg gtcattctta tgaccaatca cttgcttgat tatcccttct aaactagtcg
     9421 catacccttt ttcagggtaa tgggtcagtt cgatcagcac aatacttccg tcaactggac
     9481 gaactccctc tgctgctgca tatactttaa attggttgag ctttctatct ttcgggataa
     9541 caacgccgta aagatccgtt tcttgtattt cttcttctga ataagcaacg aattcccctg
     9601 ctaactgagt agtcgcccgg tctcgaatct cgactacttt cccctctgct ccgcgatcca
     9661 tatctgtatt tgctcgcttc atgatatcga ttgctacggt gtctccatcc attgcaaaac
     9721 cagtcgcttc ttttgggata tagatgtcat cttcttcggg atcaatcgtt acaaatccaa
     9781 atcctcgttc atttgctcga aatgttcctt cgatcaagat tggttttaaa ggaagtttca
     9841 cttttccttt tttattgaaa actatactct gttctctttc catttgagca atcgtttgta
     9901 ctagtaattt aaaatcatcg cttttttgga accctaaacc ttcagcgatt tcttccatag
     9961 aaaaactgcg tttgcgactg ctttccatga aaaagagaat tttctctttt atcgtttctt
    10021 ttgtcatatt tattcctcat tccaaggtaa tttttctaaa aattccaaga cgtctttttc
    10081 taattcgcgt cttgctgtac caactgtaat cacatgacca ctttcaggat accatcgaag
    10141 tgtcactcgc tgacggctca acttgcgtgc agtttcaaat acgccattag ggtcgatcat
    10201 ttcatctttc ccagcttgag ctaaaaatat cggctgttgg atatctgaca acctactttc
    10261 tacgattgct gcctgatctt gtatatccat taattgttgt tcagctaacg agcggtaagc
    10321 aactgctttt tcattgattt ctttctctga tttacctgct gtcttcagca cttgacgtac
    10381 gtatttttca aagttttcag gcacgtttgt ttttacagga gacaaaggcg aacagaaaaa
    10441 accgccgcca atcactgatt cttcttctag tgctcgtaca gcaaagatgc cgcccattga
    10501 taatcctaat acggcaatat cagaaaactg ctcagactgt aaaaaatgga tggctttttt
    10561 agtgtctgcc caccagcttt ctgctttttc ttgcaaaata tcatatggct ccattgttcc
    10621 gtgtccttta aaaagcggcg cataaactgt ataatctgct ttttccaaaa atcgtgcgag
    10681 cattcgaaca tcgtttggac ttcctgagta agcgtgaagc agcaaaaccg ctcgctttcc
    10741 atgttttgca taaaggggtt ttggtaattg tctcactcgt ctcacctctt tcgcctcatt
    10801 ttaccatagt ttctttcata acttcttctt aagcgttctc tttgcaataa aaaaagccgc
    10861 ccgccgtttt gcgagaggct tattcaacta cttcgaagat aaatatgcta ggacaaataa
    10921 taagatcatc cagatagctc ccataactgc agttgatcgc tgcatgacag cttcaaagcc
    10981 gcgagctttt tgtttgccga atagttgatc cgcaccgccg gtaaatgcac tcgctgcact
    11041 attttgcttg cttggctgca tcataatcgc aatgaccata acaatcgata aaacgatcac
    11101 gattcccaaa attaaattat acatatcgca gcctccttcc caaaagatat cgtctcttta
    11161 actttatcat agataaaagt ccgtgacaac tctatataaa tggaataaca gctgaaagtt
    11221 caaaactttc agctgttatt cttatgactg acgtaagacg tcttgatatt ctggtttctt
    11281 ttcaaactct ttttttgcat aagggcataa aggaatgatc tttttcccgt ctcgtcttgc
    11341 taattctact ccagtataca ctagcttttc agctaacttt tgaccacgat atgctggatc
    11401 tacaaatgta tggtcaatga tcatgatgtc tgggcccgca tctgaccatg tcatttctcc
    11461 gatctcttgt tgttcatcat tgaataaagc aagacgattt ttttcttctt tgatttccat
    11521 atgccgcact cctcaaataa ctgatatttt tttattccta ccttatttta gcagtctcct
    11581 gatctataaa caattattta tcctcgaatt acttacaaaa aagaagagaa aattaattca
    11641 tcgttattct ttgttcaata aatgaaaaaa tcactgtcca ataggttttc ggatctttca
    11701 tcgatgcttc caagtgttct gcttctggaa caagcagcag tgctttatcc cctgccgtag
    11761 cattttgaat cgtgtacgct gcttctgtag ggacaaaatg atctctttca ccatgaatga
    11821 ccaataaagg cagatggttg ctgccaagct gtctcgtagc tgatgcttgc tttaaggagt
    11881 aaccggcgta tttctgggca taatgattcg catatctcat gaccattttt ttaggaaaga
    11941 cagtgatttt acttaacata tatcggaatt ctgcataaac agaaacatat ccgctgtcta
    12001 aaataaatcc tttgaccgct gatggaagtt tttcaccact tgccatcatg atcgtagaag
    12061 ctcccattga tccgccgtgt aaaataatgg acgcattcgg ctgttcatct aagatgagtt
    12121 ggatccaggc aattaaatct agccgatcta gccatcccat tccgatgatt tctccttcac
    12181 tttctccgtg cgcacgtaaa tcaggtacaa gaacgttgaa tcctttctct gcatatcttt
    12241 tacctatatg agacatgtct cttttccctg tgcttcggta atcatgtacg caaatgaccc
    12301 atttattctg cttatttgga tggatgaaca tctgaccagc tagtttcaac ccgtcataac
    12361 ttttgattgt tttgggtgtt ccctgctccc aaaagctttc acactcttct tctaattttt
    12421 gtcgtttttt ttctactgac gtgactgcag cagggtccat caaacgatga ccagtgtctt
    12481 cataccagtc atatcctctc tgtactcccg ctcttactaa tttctttgca taaaaataag
    12541 aaagaaaatg gtaactgatc cctataatta tcagaataag aatgaaataa aaaataacca
    12601 ttgtacaccc gccgctttct gccaaactat aatctaacta ttatccaatg taccgatcca
    12661 taggattctt tattttcggc acacgtaaat tccatttctt tttcattgta tcatttactt
    12721 aaagttctcc tcaatagatt tacttgtaag agagcattta cattcgcaat aaaagccagt
    12781 atactaattt cgtgcattat aatcaagacg ctcgtcttgg actggttcgg aaacttccca
    12841 gaataaaaaa ctaagtatct ctaaagaaag gagattggaa ataatgcgaa aagtaattat
    12901 tgattgcgat ccagggatcg acgacacttt agctttatca ttagctgtca aatcccctga
    12961 tatcgaagta gttgctatta ccgtagtttg tgggaatgtc cctgcagata taggtacgca
    13021 gaatgtgctg aaatgcctgg aacgctgtga tcgtttagac attcctgttt atcaaggaat
    13081 ggaaaaacct ttgaagcaac ctttcatcag tgcacaagac actcatggta tggatggttt
    13141 aggggaaaca aatttcccga tgatcttgag aaaacaggca gaacctctcc atgccgttga
    13201 ttttttggct gattatttca aagaaaagac aaatacatca gtcattgctt tgggtccttt
    13261 gaccaatatc gcttttgctt tgaaagtcaa cccaaatatt gggaaacata tggatcgttt
    13321 tgtctcgatg ggtggaactt acaaaagcca tggtaactgt tcgccagttg ctgaatacaa
    13381 ttactggtgt gatcctgaag cagcttctta tgtattcgag aatctcaaac aaacgataga
    13441 aatggttggg ttagatgtga caagagaaat cgtcttgaca ccgactatcc tcgaatactg
    13501 ctgtcaaatg aatcctgaag agggagagta tctaaaagcg atcactcgtt tttattttga
    13561 cttccactgg aaacaagaaa gaattttagg atgtgtcatc aatgatccgt tagctgttgc
    13621 ttattttatc gagggggcca tctgtgaagg tttcaaaagc ttcactgctg tggaaactca
    13681 aggaataagt cgtggacaaa cattggttga tcgttatgaa ttttggcaga aacctgctaa
    13741 cagtaaaatt atgacaaaag tagatactcg tctttttttc cgaaagtttt taaccgttct
    13801 tttaaatgca caagaagaaa cgattatgaa agatttggaa aggttaaaga tgggctaaca
    13861 aaatgaataa aaacaaaatg gcaactcgcg tacttacagt gatggctttg tgtattggga
    13921 tcaattatat tggaggaacg attgctttat ggcttcgttt gccaatctat ctcgattcta
    13981 tcggaacgat ttttgctggt gcattgcttg ggcctgtacc tggaatgctg acaggattgt
    14041 ccagcggtat tttaagtgga gtgactacag atattttttc tctgtattat tcgccaatcc
    14101 aaattctcac aggcttgctt gctggattga ttttgccaag atatctcggc aaagataaaa
    14161 acagcttgaa actgttatta ctcgctttga ttttgtcggt acctggaacg atcgtttctt
    14221 cgatcattac cgttcagtta tttggcggga tcacctcctc aggatccagt atgatcgttc
    14281 agctattaca tgggttaggt ttaagtcaga caacaagtgt gatcttggta caggcaggta
    14341 cagactacct tgatcgtttg ttgtctatcg tcgttgtttc attagtcatc actatgttgc
    14401 cacaacgctt gatatattca aaaagaaaaa taggctgaat aaacagagct ggattagcaa
    14461 aataatgcta atctggctct gttttttcat ttattaaaaa aatcttacgc taagtaggat
    14521 aaaataattc cttgatattt ttcaatcata tccaaataat tatcgatttc aacatactca
    14581 tctacttgat gaggcaaagt aacgacacca ggcccaaaga cgacaaagtc gaaggaatgg
    14641 ttagctttcg taaattcggc tgcatccgtt gttgctgccg ctccaactaa aggtaatggc
    14701 tggctgaatt gttgctgaat gcaatgaatc aaaggagaat ctggatcagc tttgacaggt
    14761 attttgttgt aatcgatcat caattccaaa tgatagtctg tctcttgatt caattcattg
    14821 acgattgatt gcagcaatgc aatgatttta tcatttgggt attctggaat cgatcgaatg
    14881 ttcccttgga gctgcgcatg gcttgggatg ctgttgactt gattcccgcc actgatcaat
    14941 gtaacattat ggattgtttt tcctaggaca ggattctcga ttgtttcagc gaggtgattc
    15001 atttcagcat ttgctttcgt aatgaattca tttaggtgat tgattgcatt ataaccttgg
    15061 tctggcatag agctatgcgc ttcttttccg tgcgaggtga ctgtatagtt gattgatccc
    15121 atgtgcgtat acatcaaact gtaattagtt ggctctccga tgatcaaggc atctaagtca
    15181 tctacatagc ctgctttcgt gagctgttcc ccacctagtt cacccacttc ttctccgaca
    15241 gtggctaata atttgactgt tccgttaaat ggttttcctg actctttcag ttcgatcatg
    15301 gcaatcacca ttgctgccaa gccactcttc atatctgtag caccacgtcc gtacaaacgg
    15361 ttcccatgga tttctgctgc aaagggtgca taggtccaag acgattcatc accagccgcc
    15421 acgacatcca tatgcccaga taggcctaac acttttcctg attggccctt ttggtaacga
    15481 gcaatcaaat tatctctacc atctctgtaa gaaacaatct ctcctgttat gtcatgcttt
    15541 gccaaaagct tattcaaata ggctgctact tcaccttcgt ttccattcac tgatttaatt
    15601 cggatgatct cttgtaaaat cgcgattttt tcttcttttt tcatctaatc ctctcccttg
    15661 ccatttcttt ttattgtatt tgagtgtgct cgaattttca aataggagca accaaagtaa
    15721 aaatgaagaa agagagccac tttatgaaaa atatgctgct acttccctcc tattttattt
    15781 atacgagagg acttgctcat agattcctat tctactgttc tatccttaaa agctcttata
    15841 tgatgctagt tattgttagc tttccttttt caaggatgat cttttcttgt accattcttt
    15901 gtaaacatca tatacagaag ctagttgcaa aaaaaactct ctacctgctg atatt
//
LOCUS       DANJJH010000065        15804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000065 DANJJH010000000
VERSION     DANJJH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            28..1320
                     /locus_tag="PWS53_001953"
     CDS             28..1320
                     /locus_tag="PWS53_001953"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL0872166.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2056..2919
                     /locus_tag="PWS53_001954"
     CDS             2056..2919
                     /locus_tag="PWS53_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0872167.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3048..4670)
                     /locus_tag="PWS53_001955"
     CDS             complement(3048..4670)
                     /locus_tag="PWS53_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0872168.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            4919..5440
                     /locus_tag="PWS53_001956"
     CDS             4919..5440
                     /locus_tag="PWS53_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0872169.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5626..7356)
                     /gene="spxB"
                     /locus_tag="PWS53_001957"
     CDS             complement(5626..7356)
                     /gene="spxB"
                     /locus_tag="PWS53_001957"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL0872170.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7521..8339
                     /locus_tag="PWS53_001958"
     CDS             7521..8339
                     /locus_tag="PWS53_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL0872171.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8442..8639)
                     /locus_tag="PWS53_001959"
     CDS             complement(8442..8639)
                     /locus_tag="PWS53_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872172.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            8808..9386
                     /locus_tag="PWS53_001960"
     CDS             8808..9386
                     /locus_tag="PWS53_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0872173.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            9481..9618
                     /locus_tag="PWS53_001961"
     CDS             9481..9618
                     /locus_tag="PWS53_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872174.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(10431..10934)
                     /locus_tag="PWS53_001962"
     CDS             complement(10431..10934)
                     /locus_tag="PWS53_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0872175.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            11293..12318
                     /locus_tag="PWS53_001963"
     CDS             11293..12318
                     /locus_tag="PWS53_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0872176.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            12641..13519
                     /locus_tag="PWS53_001964"
     CDS             12641..13519
                     /locus_tag="PWS53_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL0872177.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            13995..14111
                     /locus_tag="PWS53_001965"
     CDS             13995..14111
                     /locus_tag="PWS53_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0872178.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            15039..15377
                     /locus_tag="PWS53_001966"
     CDS             15039..15377
                     /locus_tag="PWS53_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872179.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            15471..15701
                     /locus_tag="PWS53_001967"
     CDS             15471..15701
                     /locus_tag="PWS53_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872180.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         5024 a         2665 c         3069 g         5046 t
ORIGIN      
        1 ttaaataata tagaacgagg tgttcacatg tcatctgtag tagtagtagg tacgcaatgg
       61 ggcgatgaag ggaaaggaaa aatcacagac tttctcagtg aaaatgctga agtgattgca
      121 cggtaccaag gtggcgacaa tgcagggcac acaattaagt ttaacggtgt gacttataaa
      181 ctgcatttga ttccttctgg gattttttat aaagacaaga tcagtgtgat cggaaacggc
      241 gttgtagtca atcctaaatc attggttaaa gaattagctt atttacatga aaaaggtgta
      301 gaaacaagta atttgcgtat ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa
      361 ttggaccaat tacaagaaga tgctaagggc gaaaataaga ttggtacaac gattaaagga
      421 atcggacctg cctatatgga taaagcagct cgtgtaggta tccgtattgc agacttatta
      481 gataaagaaa tttttgaaga acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt
      541 gtaaaaatgt ttgattcaga accacttgat tttgacgata tttttgaaga atattatgaa
      601 tacggacaac aaatcaaaca atacgtcaca gatacttccg ttatcttgaa tgatgctttg
      661 gatgcaggca aacgcgtgtt gtttgaaggc gcacaaggtg taatgctgga tatcgaccag
      721 ggaacttatc cttttgttac ttcttctaac ccagtagccg gtggggtaac aatcggaagc
      781 ggtgtagggc cttcaaaaat caataaagtt gtcggcgtct gcaaagccta tacgtcacgt
      841 gtcggtgatg gaccatttcc aactgaatta tttgatgaaa caggagaaca aatccgcgaa
      901 gtaggacgag aatacggtac gacaactggt cgtccgcgtc gtgtaggctg gtttgattct
      961 gtagttatgc gccattctaa acgagtttca ggtattacca acctatcttt aaactcgatc
     1021 gatgtattaa gtggattgga aacagtaaaa atttgtacgg cttatgaatt agatggcgaa
     1081 ctaatctacc actatccagc aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa
     1141 gaattgccag gctggtcaga agacattaca ggatgcaaaa cacttgcaga actgccagaa
     1201 aacgcacgta actatgtacg tcgtatctca gagcttgttg gtgtacgtat ctcaacattt
     1261 tctgtcggac ctgatagaac acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa
     1321 ggataaaaac gacgactgaa tcaggacgtt cttaccagag cgagtcgaag tgtttatcta
     1381 tacgataata ataacttgaa cactgtttat tcagaataag ggactgtgac aaaaaccgtg
     1441 tcacagtccc tttttccgaa caaacggtgt tcaaaagctt gcatctacgg cactaagccc
     1501 aatcagatga aaaatatgga gtgctatttt tatctgattg ttcttactgc ttgatgcaga
     1561 gcagcttttt cacaacctct ccactaaacg gtgttctcct gccaactcct cggtcacaag
     1621 tcacaatatt caacaaacat gagaagctgt tttttgaaat tgttccttgt gactcggagc
     1681 taaacggcag gctcacaacc tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta
     1741 gctcaaaaaa cggcaaaata tgaggaacta ttttttccgt ttttttctct aatactcagg
     1801 tcaaaacgct gatttcacaa cctctgattc acggtgttcc agaagtagct tctacggaaa
     1861 taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
     1921 aagctgacca cttctgtcac aacctcttat tttgattaga acttgcgaaa ataaaccggt
     1981 taaagtgtgt ctagcgcaag agatttgcta taatagacga taagggtaaa atgtaacgag
     2041 aaggggttga aaaatgtgta tcaaattgta acagattctt gttgtgatct gccataccat
     2101 gtattagcaa aagaaaaagt tgatttttta tcaatgaatc tccaattgaa tgatcaagag
     2161 ttagtagatg acctcggaaa gacattcaat tatgaccgct acatgaatca gctgaaaaat
     2221 ggtgcgatgc ctacgacatc acaaatcaat gttggccgat atttagaatt tttccgttct
     2281 tatgcggaaa aacagattcc tgttttatat gtggcttttt ccagtggatt aagcggctct
     2341 tacagtagtg cgcttcaagc ggttgaattg ctgaaggaag aatatgaaga ccctgagatc
     2401 tatgtttttg atacaaaagc tgcaagtatt ggggaagggc ttttagtatt ggaagctgta
     2461 agattgaaaa aagaaggtaa aagcatcgaa gaaaccctag agtggctgga aagtaattat
     2521 ttgaaagtcc attcatgggt aacagtagac gatctgaaat atctagaacg aggcggtagg
     2581 atttccaaag caagtgctac tcttggcagt ctattgagtg taaagcctat tattacggtg
     2641 gataaagaag gaaggctgac gaatgttgac aaagttcgag gaagaaacaa gtcgatccag
     2701 acaatcgtca atgagacggt aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg
     2761 gcttacgctg gggatctaga gagcgcggag aaggcaaaac ggcttttgga agagcagata
     2821 aagatgaaag atgtgaagct ttatccctta ggaccaacga tcgccagtca cacaggctat
     2881 ggctgtatcg cgattttttc gatgggaatc agtcgataaa agcagtaact cattctgtaa
     2941 aaaagcccag aaattgattg taacaaaaac agcgactctt aaaagataga tgagcatcct
     3001 atctcttaga ggtcgctgtt tttttatttt ttaagctaaa taggccatta tgcccataac
     3061 tcgttcaatt gtttttgaat atctgcattt tccaaaaatt catcatatgt cgtatcagct
     3121 cgatcgatca cacctttatc cgaaacggca atgatgcggt ttgccaatgt ttggataaat
     3181 tgatggtcat gtgacgcaaa taagattgag ccagtaaatg ccatcaagcc gtcattcaat
     3241 gctgtgattg attctaagtc taaatggttc gttggatcat ctaatacaag tacgttagct
     3301 ttcgaaagca tcatttttga aagcatgcaa cggactttct ctccaccaga taaaacattc
     3361 actggtttca atacttcttc accagaaaac aacattcttc ctaagaagct tcttaagaag
     3421 gtgttatcat cttcttcttt acttgcatat tgacgcaacc agtctaaaat cgttagatca
     3481 gtatcgaatt ctttcgttgt atcctttggt aaataagaat tacttgttgt cacaccccaa
     3541 cgtactgttc cagtatctgg cgtaatttca cccatgataa ctttgaatag cgttgttgtt
     3601 gttatatcat tttctgcgac gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata
     3661 tcatctaaaa ttttctttcc atcaatcgtt acacttacat tttctacctg caataagtcg
     3721 tttccaatct cacgttcagg tttgaatcca acgaatggat aacgtctaga agaaggttgg
     3781 atatcgtcta aagtaatttt ttcaagcatt tttttacgtg aagttgcttg ttttgatttt
     3841 gatgcattcg cactaaagcg ggcaataaat tcttggagtt ctttgatttg ttcttctttt
     3901 ttggcatttg cgtttgcttg aagtttagcc gctagttgac tagattccaa ccaaaaatca
     3961 taatttccta cataaagttt gattttacca aaatctagat ccgccatatg tgtacatact
     4021 ttgttcaaaa agtgacggtc atgggataca acgatcacag tattttcaaa attgatcaaa
     4081 aattcttcca gccaattgat cgattggata tccagaccgt ttgttggttc gtctagcaaa
     4141 agaacatcag gtttaccaaa tagtgtttgt gctaataaca cttttacctt ttgtccacca
     4201 gttaattcac tcatttgctg atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa
     4261 acagctgctt ctggttcagc ttcccatcca ttaagctcag caaattctcc ttctaattca
     4321 gctgctcgaa taccatcttc gtcagtgaaa tcctctttca tgtaaatcgc atctttttct
     4381 ttcagcactt catatagacg cttgtgtccc atgatgactg tttcaagtac agtatagtct
     4441 tcatagtcgt aatggttctg tttcaatacg gccatccgtt catctggtcc catagtaacg
     4501 gaacctgtgg aaggctgcag atcccctgat aacactttaa ggaacgttga tttacctgca
     4561 ccatttgcac caatcaagcc atagcagttt ccgggggtaa atttaatatt cacatcatca
     4621 aatagtttgc ggtctgaaaa gtgcaaactc acatcattta cagtaatcaa tttttttcct
     4681 cacttttatc attattagtt ttgacaagtc ttatagaatt atatagggga aaggtaaaag
     4741 attcaaggca atcgaacatt ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat
     4801 aaaagcggag cgaaaatagc tactatagga tttattagtt tgcaacagca taatttttga
     4861 cgttatttat aagagggagt actataaaac tgtaaaagaa atgaaagaag gattgaaaat
     4921 ggatgaaaaa agaaccatcg actggggctc attgattttg ggaattttat ttgtacttgt
     4981 ctcattgtta tcatttcaag accctgtcgg aaatttagtt gcaattgtag tagtctttgc
     5041 gatttttgcc tttattaaag ggatatttga actgtttgta cggaacagat taaaagagct
     5101 aacaggatat aaaggaaaga tgcccttaat aatcgggatc atcgatattc tagttggcgt
     5161 ctttttctta ttcaatattg gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg
     5221 gtttattgct gattcagttt tagcgttatt tactgctgac ttggcaagag gagtaagtga
     5281 aggatattat tggtttacga tcatcgtcaa tattttagga attttattag gaattttctt
     5341 attgtttaat ccaatttctt ctgcgttgac attaagcttt ttagttggat tctattttat
     5401 gttatttggt atcacacata tcgtctatgc atttagataa gtaaaatcaa acaaataaaa
     5461 aagccggctt ccgattacag ttttactgtt gtaattggaa gccggctttt ttatcatgga
     5521 caaatatgca cgaggtgatt tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc
     5581 tatatacaca aattttatct taatactcga agcatagcgc agttgtcaca acctcttttc
     5641 atataattca ttcaatactg gcaaatcatg gacttcatat ttttctttga atgcttggat
     5701 ttcttcttca gaatattttc ttggatctaa atgcagatct tctactggca atgggcgctt
     5761 gttattgatc ttcacatcga ttactacagg acgatctgat tttgcagctg catcaaaggc
     5821 aaatcgaagt tgcgagtatt ctgtgactgt aaagctattt gccccgagcg cttctccaac
     5881 tttaccgaaa tctgcatctt ctaaaaatac accaaatttc ttttgttccg tatcttcttg
     5941 ttctgcttcg atgaaaccaa aagaatcatt cgaaaagaca acattaataa ttggaagctg
     6001 atatttgact tgtgtaatga tatcttgcat gaccatagca aacccgccat caccacttaa
     6061 ggtaaatact tgtttttctg ggtagcttag ttgtgcggca attcctcctg gcacaccatt
     6121 acccatagtt gcgaaccatc cagatgtcgt atgtttttgt ttaccgttca tattcaataa
     6181 acgaatagaa tggatagtcg tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc
     6241 aatacgattg atttctttat agacagcctc tggacgaatc ggttcttcct ctcgatcttc
     6301 aaaagatttc aaccaattta cccatttttc tttattttct tgatttgcta ggtaccagct
     6361 atcagcaggg cgagcatccc ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc
     6421 gaggatcgac aaagatgttt catggcgtcg tccaaatttt gacgcatcga tatcgatttg
     6481 gataaattca gcagttggat taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa
     6541 taagatcaga tcggcttctg ccaatgcttc atttccgggt ttagtagcga cacgtccagc
     6601 aaaaccaaga aagttttcat aatcatcagg aataattcct tttgctagaa cggaagcgac
     6661 aacaggaact gaaaaatgtt ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc
     6721 ttgaccaaca taaagaactg gtttttttgc tttttcaaga taaggcaaag cagctagcaa
     6781 atctttttct tctggtaata tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg
     6841 ttcgtcaatt tcttcaaaac caagatcaac agggatagtc acgatagcca cgcctttatg
     6901 ctcataggcg gctttaatgg cttcatctac tacatgcgga agactttgtg gtgtcatgac
     6961 tgtacgattg tatacgctaa catctgcgaa aataggattt tcatttagtt cctggaaaga
     7021 gttatagttc atggatgtag aagcaacttg tcctaagaac gctactagag gcacatggtc
     7081 catctgtgca tcatacaaac cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa
     7141 gacagcgccg actttccccg ttaattttgc atctgcagcg gctgcgagtg cgccgacttc
     7201 ttcatgacga acttgaatgt agttgatttc ggttttttct tgatatagcg catccataat
     7261 tgaattgaaa gatcctccgg gaatgccgta aatatggtcg ataccccagc tttcaagaac
     7321 ttttaccatt gcgattcctg cattgatttt tgacatggtg tttcactcct ttagcaatga
     7381 tgatacataa attttttgta tctagagtat agaacttttt atcgaaaatc ccaactaatt
     7441 cgtcttttca caaaaatgta gtcatgtttt tgggaatatg ctagagtagt acatagatag
     7501 attcaagaga ggggaatggc atggttgaat ggctattgag acttgaacct tggcagcaag
     7561 cacttgctgg gacagctttt acttatttta tgacagctct tggtgccggg ttggtttttt
     7621 tctttaaaga aatcaaaaaa gaagtgctaa acttgatgtt aggatttgct tctggtgtca
     7681 tgattgctgc aagtttttgg tctttgttag atccggctat tacaaaagca gaagaaaatg
     7741 gagatattgc ttggctagtt gtaagtattg ggtttggttt aggcggtgtg tttttatata
     7801 tggcagataa aacactgccc catatgcatt ttggaccaca acatgaagca gaaggattgc
     7861 ctacccattt aaaacggacg atcttgcttg ttttttctat tacattgcat aacattcctg
     7921 aaggtttagc cgtgggggta gcattcggtg cagcagcaac tgcggataat ccgacagccg
     7981 ctatattagc cgctgtttcg gtggcgctag gtattggtat ccaaaacttc ccagaaggag
     8041 cagctgtatc aatcccgttg cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg
     8101 gacaagcttc tggaatcgtt gaaccaattg ctggagtgat tggagcgcta ctagtaacga
     8161 aagtggagct gctgcttcct tatgcacttg cttttgccgc aggagcaatg atttatgtcg
     8221 tggtggaaga attgatccct gaagcacagc agacattgtc cagcaaacgt cattttgcag
     8281 tttttggcgt catgagtgga tttatcatta tgatgatttt ggatgtggca ttaggataaa
     8341 taaaaaaaat atatatgata tatcaaatag ggccgcgaac ttatatcgcg accctatttt
     8401 tttagtaagt tatatcataa tgaagtgaat ggtatcatac attaataata gttttttcta
     8461 tgtgtatttc ctttgatcca gcgaacaagc cagatgataa gtgcgattgg gattagaact
     8521 ttgaccgcaa accataaaag cccgcttagg acactgacaa aaagactgat gacgaagcta
     8581 gctgcaatta gcactaaaag aaccatgaag atatttaaga acatattttt ttgacgcata
     8641 caaaaccctc ctcttgttca ttctttacta tagaaaaagt ataacaggga ttgtttaaaa
     8701 acacctccaa ctgaagactg atttttactt catctactta aatgatcaaa caattgcttt
     8761 ttctctggtt tatcatctaa aatagagcgg aaaaggagga gaatatcatg cctaaaaatc
     8821 gtcttgaagc atttacagat gctgtaatcg cgattatcat gactatttta gttttagaac
     8881 ttcatgaacc taacggagac acgtgggagg cgctcgctgc attaggttac cgatttttta
     8941 tctatattgt cagttttatc attttagcga tttattggaa caatcatcat catttatttc
     9001 aagttgtaca taagataaat ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc
     9061 tttcgctttt tccgtttgca acttcatgga tcagtgagta catcaatagc ctggctccag
     9121 aaatgactta tggttttgtt accttgaccg ctaatattgc gtattatatc ttggcaaaag
     9181 aacttgtcaa agcaaataaa ggccacgaga aagcattgaa ggcgatgttg aataattaca
     9241 agaaatcgta tctatcgatt ggtttgaatg tgttaggaat tgtcttaggg tatttagtag
     9301 cgccagtagc cgtgctgatc gcaaacatcg tgattctagc tgcttggatc atcccaagtc
     9361 gtaccatcga atcacaatat cggtaaaatg atccgtgttt aaaatttaag agaaaaatat
     9421 agaaaaaata aataaacgat caaatatccg agtgatacta gtattcaaag agggatgcag
     9481 atggatcaaa aaagaattca tgtaggactg attactagtt ttgtcacgat gcttatttat
     9541 ttggatcaag gtcatttgac ttggacagta gttggtgtcg taggattttt gacattattc
     9601 acagcaataa aaaaataaac atctttatgt aatctgatgt tctatctatt taagaaaagc
     9661 atgtttacag cagtatgatt ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt
     9721 tagaaaagtg tatcgaaaaa tagatagtta gtttttccac agtggataac ttcgtaagag
     9781 ataaaaataa aaaattagtc aaagaagggc gtatagattt cttttgatag gagatataca
     9841 acagaaatga ataaatatta acgtgttttc aaacagtata aaacggtgtg gtttttggaa
     9901 taaaaaaata acatatctca ttttacgctt ttgttatttt atacttaaaa aataacgaca
     9961 cagaaaatac cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt
    10021 gaaatgcctt caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag
    10081 cctaaataag gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt
    10141 atagaaaaat aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga
    10201 gatattgact aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt
    10261 gttaaataag gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat
    10321 atagtagatt agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc
    10381 atagatacac attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga
    10441 cggacagttc gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc
    10501 aaaataatgg ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag
    10561 gcccataaac ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg
    10621 ccaataccga ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct
    10681 aattttttag aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa
    10741 aagacaatca agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta
    10801 gaaaaaggga cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat
    10861 gatagcttga ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt
    10921 gatacatacc gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa
    10981 aatcataaaa aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct
    11041 tttttttgtt gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag
    11101 ttgctatctt ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca
    11161 agttgtttac tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt
    11221 aatgctgaca aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga
    11281 aggagggata gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt
    11341 cgtatcatga atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat
    11401 gattttttga tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac
    11461 ataccgatga aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct
    11521 ttcgttttgg ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg
    11581 gttgcttttt ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg
    11641 ttttttgcga tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg
    11701 atcgtctcac tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca
    11761 aaattactgg attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt
    11821 cacgaagagc ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc
    11881 gtttatgtca gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca
    11941 tcgattttgc tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt
    12001 ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg
    12061 cagaccattt ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat
    12121 tatgcagttg ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt
    12181 ggagcatact tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc
    12241 atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt
    12301 cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt
    12361 tgtcacaacc tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca
    12421 cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt
    12481 gtttatgatg gtaactgaat aaactttcct gagatggtca atgctttcct tgaaagtctt
    12541 acagatattc tttcttcata gaaagtttaa gttttatagt tagtgtacaa taaaagtatg
    12601 tttataccat atggtgtccg gataatcagt catttaaaca atgaggttca aagatatgaa
    12661 aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa attctggcag aaactttaac
    12721 tcttcccaaa gtcgttatag agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt
    12781 gtttgagcaa tatagtcatg aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa
    12841 aatagacaaa gtctatgaag aggaaccttt tgcagcatta gctatttatc tatatgcagc
    12901 ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt ccaagaacta tctatttggc
    12961 aacaatgaat gcttttacta gatttattca tgaacatttc caagtaacag gaagctatcg
    13021 ctttgaccgt ggtttttgga tttggcatta cactagtggc ttgatctttc ggattggtga
    13081 attggagtac gaaatgacat attttccgaa agggtacacg gatctaagat tagaaggaaa
    13141 gcagtgcctt tctcttcata ttccttccga tgcagattta tctcagaata agataagaga
    13201 tagttacgaa agtgccattc gtttttttga actttatttt cctgattacc attaccaagt
    13261 gatgtacaca gatacttggt tactatcacc caatcttact aaatggttga aaaaagagtc
    13321 aaaaatctgt ctttttgcag ccgattatcg attattatca gttgatgaac aggacgatag
    13381 tggagtccct tggatctttg gacgagtaga tgcccaaata catgattatc cagaaagtac
    13441 gagtttacag cgacaagcaa aagagcaatt gcttgcaggc gagcacattg gctctggctt
    13501 aggtattctt acttcataag tttagtactt cgaaatagtt taaaagagcg tgagacaaaa
    13561 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact
    13621 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct
    13681 tatttcttgg agttaaacgc ttctgtccca gcctcttttt tagtgttcta tcgaacacac
    13741 agtacgttca ataggtaaac tagctataac aatagatatt aaagttaacg gacaagaggg
    13801 ccttttttag gtagctaaaa cgtaataatg aaaatttatt tccagagata ggagtcaatc
    13861 agctagtctg atcgactgtt ttaacaagtt gactttcatt ttaacttttg ataccattta
    13921 aaacacaaat cgtaattgtt tcgatttgtg ttttttagca tgactacaat caaagaagaa
    13981 agtgggtcgt ccatatggaa aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc
    14041 caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg
    14101 ggaaaaggta agaagaagga aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta
    14161 ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat tttccatttt
    14221 taacatatga taaattggta gaatctttct tttcaaattg ataccattcc ctaattccag
    14281 ttatatagtt aactacagaa ccacaaagca cgtacgttta taaaagtatt atctgtagtt
    14341 atttttcaat tccctttcaa cctcttattt tgaccagtag tattgatcac tttacctttt
    14401 aacacctttg ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa ctacataatc
    14461 aaaggttttg cgaaatattt catgaaatat ttcatgtaga agcgagcaat acggttgcaa
    14521 ataaccatta tcgtttaata agcagttacg aaagctccca atatctctaa cgttcagctc
    14581 actccatttc ttttcttcaa aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa
    14641 gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt
    14701 gctagaataa catcaaggaa taataagttt tggttttcag ataatagtcg ataagttgtg
    14761 tattggaatt ttagatacag taataaagta tttttcatga aattttgtga gaatcactat
    14821 tacaagtatc tttaataaaa ttaatggtta aggaggaact taaagtagaa tgtaaatgaa
    14881 tagttgtagt cagtcgattg tgatactaga ctcaaagttg caggaataag tttttcaatt
    14941 tgtgattgga agataaaaaa tgaaaaagaa gggctttttt ttatactttg ttgattgtag
    15001 tgtttatttt ccctctgttt agccaagtaa cattttctat gactcaacca gtttcaagtg
    15061 aggaacatga taaccttttt cgtgctacag aagagtttaa gaaatatcag gaaaatattg
    15121 ttccggagac tccattagct agtgagccaa ttactgatat tgatggaaac caaataggat
    15181 ggtatataca gtacgaagtt atggtggtaa ataatgactc aatagtaaaa aatacagtag
    15241 gcttagagct aacaccgatt gaaaaggtga gttcattgtt aacgtatatt tatactgatg
    15301 aaagtgacga gactgagatc attttaacgg attactcaaa aattgctact gataaaaagt
    15361 atatattgtg gatgtagaca ctagtgaaat actttatgat atagaaattg atgaaaattt
    15421 actagatgta gcacaagaaa tcgatcatca aaagcaggaa gctattcgat atgctggtag
    15481 aaaagggaaa agaaaatatt tattacgtga atgtagcaaa agttagagaa gatgaaaatg
    15541 agtggaaaga attcaagagt cgctactcaa ttaactcgac tccgacattt acagtttaca
    15601 gagaaggatc gatagaaaaa actgtttttt ggactaaaga aagtggaatg tctttagctg
    15661 aagtagaaga gtttttggat tatgttagta tgcaacagta agggagttct tattatgaaa
    15721 aattttgaca catattatgt tattgttttt tcgatatctt tgctgttaat attactaact
    15781 ttaatgcagt ataaagagta tcca
//
LOCUS       DANJJH010000066        15700 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000066 DANJJH010000000
VERSION     DANJJH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(12..1535)
                     /locus_tag="PWS53_001968"
     CDS             complement(12..1535)
                     /locus_tag="PWS53_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0872181.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="PWS53_001969"
     CDS             1787..2968
                     /locus_tag="PWS53_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0872182.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWS53_001970"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="PWS53_001970"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL0872183.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="PWS53_001971"
     CDS             complement(5053..7764)
                     /locus_tag="PWS53_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872184.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="PWS53_001972"
     CDS             complement(7774..8475)
                     /locus_tag="PWS53_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872185.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="PWS53_001973"
     CDS             8661..9260
                     /locus_tag="PWS53_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0872186.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="PWS53_001974"
     CDS             9341..10354
                     /locus_tag="PWS53_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0872187.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="PWS53_001975"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="PWS53_001975"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL0872188.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="PWS53_001976"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="PWS53_001976"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL0872189.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="PWS53_001977"
     CDS             11802..13175
                     /locus_tag="PWS53_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0872190.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="PWS53_001978"
     CDS             13532..15676
                     /locus_tag="PWS53_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0872191.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4619 a         3134 c         2918 g         5029 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac
//
LOCUS       DANJJH010000067        15518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000067 DANJJH010000000
VERSION     DANJJH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            138..1475
                     /locus_tag="PWS53_001979"
     CDS             138..1475
                     /locus_tag="PWS53_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL0872192.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="PWS53_001980"
     CDS             1523..3652
                     /locus_tag="PWS53_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL0872193.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="PWS53_001981"
     CDS             3627..4316
                     /locus_tag="PWS53_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872194.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="PWS53_001982"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="PWS53_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="PWS53_001983"
     CDS             complement(5546..5740)
                     /locus_tag="PWS53_001983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872195.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="PWS53_001984"
     CDS             complement(5790..6230)
                     /locus_tag="PWS53_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0872196.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="PWS53_001985"
     CDS             complement(6234..7079)
                     /locus_tag="PWS53_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL0872197.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="PWS53_001986"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="PWS53_001986"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL0872198.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="PWS53_001987"
     CDS             complement(8703..9650)
                     /locus_tag="PWS53_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0872199.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="PWS53_001988"
     CDS             complement(9873..10223)
                     /locus_tag="PWS53_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0872200.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="PWS53_001989"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="PWS53_001989"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL0872201.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="PWS53_001990"
     CDS             11646..11966
                     /locus_tag="PWS53_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0872202.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="PWS53_001991"
     CDS             11988..12452
                     /locus_tag="PWS53_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0872203.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="PWS53_001992"
     CDS             12657..13262
                     /locus_tag="PWS53_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL0872204.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="PWS53_001993"
     CDS             complement(13301..14590)
                     /locus_tag="PWS53_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL0872205.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="PWS53_001994"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="PWS53_001994"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL0872206.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4846 a         2899 c         3182 g         4591 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaa
//
LOCUS       DANJJH010000068        15505 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000068 DANJJH010000000
VERSION     DANJJH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            88..438
                     /locus_tag="PWS53_001995"
     CDS             88..438
                     /locus_tag="PWS53_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL0872207.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            537..1067
                     /locus_tag="PWS53_001996"
     CDS             537..1067
                     /locus_tag="PWS53_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0872208.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1221..2372
                     /locus_tag="PWS53_001997"
     CDS             1221..2372
                     /locus_tag="PWS53_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0872209.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2481..4520)
                     /locus_tag="PWS53_001998"
     CDS             complement(2481..4520)
                     /locus_tag="PWS53_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL0872210.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4578..5135)
                     /locus_tag="PWS53_001999"
                     /pseudo
     CDS             complement(4578..5135)
                     /locus_tag="PWS53_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5674..5880)
                     /locus_tag="PWS53_002000"
     CDS             complement(5674..5880)
                     /locus_tag="PWS53_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872211.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5896..6510)
                     /locus_tag="PWS53_002001"
     CDS             complement(5896..6510)
                     /locus_tag="PWS53_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL0872212.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6657..7340)
                     /locus_tag="PWS53_002002"
     CDS             complement(6657..7340)
                     /locus_tag="PWS53_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0872213.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7449..7907)
                     /locus_tag="PWS53_002003"
     CDS             complement(7449..7907)
                     /locus_tag="PWS53_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL0872214.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(7991..9754)
                     /locus_tag="PWS53_002004"
     CDS             complement(7991..9754)
                     /locus_tag="PWS53_002004"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL0872215.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9975..11435)
                     /locus_tag="PWS53_002005"
     CDS             complement(9975..11435)
                     /locus_tag="PWS53_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872216.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="PWS53_002006"
     CDS             complement(11643..12569)
                     /gene="glsA"
                     /locus_tag="PWS53_002006"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL0872217.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12592..14019)
                     /locus_tag="PWS53_002007"
     CDS             complement(12592..14019)
                     /locus_tag="PWS53_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0872218.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14050..15264)
                     /locus_tag="PWS53_002008"
     CDS             complement(14050..15264)
                     /locus_tag="PWS53_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL0872219.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4435 a         3094 c         2664 g         5312 t
ORIGIN      
        1 ttgttttttt gaataatcgc tttttcttct ctttttctta tatactataa gtagtaaata
       61 atttgaaaaa aagaaaggtg actgattatg gcgcaaaatc gtatttttac gacaagtttt
      121 gcaagcgttt atcctatgta tgtagcaaaa gcggagagaa aagatcgtat aaaagaggaa
      181 gtagatgaaa tcatttgttg gctgacgggt tattcccaaa cagaactaga atatcaacta
      241 caaaaacaag tgactttcga acaatttttt gcggaagccc ctgtccccaa tccttcacgt
      301 tccttgatta caggtgttgt ttgtggtgta cgagtagaag acatcgagga taacttgatg
      361 aaagaaattc gttatttaga taaaatgatc gatgagctag caaaaggcaa aaaaatggaa
      421 aagatcctca gaagttgatt tttccagaga tattcttctc cttcgctcat tttgtaacat
      481 ttaatttggt aaaatgacta gttcacgtta aaataagtca aaggaggttt tcttatatgt
      541 ttgagacgat taaacaaaat attcaaaaat acgccttatt aagagctgct gtttatatta
      601 tttctgggat tgcgattgtg ctaaaccctt ctgctgtttt tcattttatc ggttatctga
      661 tcaccgccta tcttgttttg cttgggttgt tgaatctttt tgaggcttac cgttttaaaa
      721 ggaaaacagg ttcttggagt atcggtctgt tcagtggtct ttttttcctg atcttagctc
      781 tgatcgtcta tgcttttgca cctgcaatcg tttctatcct gccgattctt cttggattag
      841 ctgttatcat caacagcttg ttccagttgt tcttcagcat gaatacaaaa gctaaaagtt
      901 ggagtgttta tagtagtatt ctgttgttga ttggcggatt cgtcttattg ttcaatccat
      961 tcaaaagttt gatgcttcta ttccaaatat tcggctttat tttgattttc atgggcatcc
     1021 aggagattat caatttcatc agaataagaa aaatgactat acgttaatca tttttagtta
     1081 ttaaaataga ccgtataaaa accaacaatc caaaaaaatc ttttggattg ttggtttttt
     1141 tgtgagtata ttttcctatt ttttcaataa tgtatgttat gctaagttcg ggctatattt
     1201 tatacaaagg aatgatgaga atggaattta ttggtatttt atgcctgata ttgatttcaa
     1261 caacgattgg ctcacatatc tcgcggagat taggtatccc agcagtgatt ggacaattac
     1321 ttgttggtgt tttgctaggc caagctggat tgaattgggt caaacctgat attctagtcc
     1381 atgacttttc ggagattggt gtgatcttat taatgttttt agctggtctg gaaagtgatt
     1441 tatctctttt gaaaaaatat ttccgacctg gtatgttcgt tgctttgctt gggatcatct
     1501 tcccggttgt ttttggctgg ttgacaggca aagcattcca agtttcggga aatgaagcga
     1561 ttttctttgg gattattctt gcggcaactt ctgtcagtat ttcagtagaa gtattaaaag
     1621 aactaaacgt agtgaataca aaagaaggat caacaatttt gggtgcttct gtggtagatg
     1681 acattttagt cgttttagtt ctaagtttca gcctatcttt tctaacgggg gaagcttcta
     1741 gcaatgcttc tttaccgctt gttttgattg aacagctaat ttattttgtt tttattttct
     1801 tattagtcaa atggattgcg cctttcctac tttcatttgc agaaaaagtg tacgcaaatt
     1861 cttctattat catcgtatcg ctagttattt gtttaggaat gtcttacgta gcggatcttg
     1921 ttgggcttag ctctgtcatt ggtgcttttt ttgctgggat agctgtcagt cagacaaagg
     1981 ttaaagaaga agtataccac aatgtagagg ctttaggata tgcagtattt ataccagtat
     2041 tttttgtcag tgtaggatta gaagttgatt tttctgactt tagtgaacaa ctactcttta
     2101 tttctatatt gacgcttgta gcgattttga ccaaattgct tggaggttat tttggtgctc
     2161 agattgccgg tttttctcaa catagttcat tgatggtcgg tgctggtatg atttcgcgtg
     2221 gagaaatggc tttgatcatt ttacaaattg gacagcaaag tcaattgatc gaaccacagt
     2281 actattcacc tttagttatc gtgattttat taagtacact tatttctcca cttgttctca
     2341 aatattttac aaaaaaagtt tatgaagtgt gagtaaatca aaaagaagtt gtgaaagaga
     2401 catcgtgaaa accgtttatt tgaaaaaagg actgtgacga taacgccaca gtccttttac
     2461 tgtacttaaa aagcaatttt ttattgataa ttttggttga ggtattgcaa tagttccgat
     2521 gctcgtttgg ttgctgaatc atcatcttct ttattttcca acatgctgac catcatataa
     2581 ccttggttat caagattgaa agcaaacaag aaactgttct cttgcccctt tacatcctgt
     2641 ttttctttga tttccgctgt accagttttc gctgccaacg gaatccctaa agcagaaaga
     2701 gaatgtgctg taccatttac atcttgcaca acttctctca gatctggcac gatcgtttgt
     2761 actgctgttt cgctgatgac attcttctta tcttttgtct ctttatctgc aatcaattta
     2821 ggatagacaa gtgtgccatt gttggtaaaa acagaataca ttgctgcttg ctggatagga
     2881 gtaattagaa gttcgccctg tccatatcca gtatcagcta gcaagatatc tgagttaaag
     2941 ctatcttcat tagaaatttg tgctggattc atactaattg gcaaatcaag gtcttcacca
     3001 aaaatgaatt tatctaagcc tgtacgaaat tttttctcac ccattttcaa cgtttcttgt
     3061 gccgcatata tattatcgga atagatcaaa gcagttttta agtctacttg tgatacatca
     3121 ctactgacac gcgttgtttt aaaagatccc caagaactgt ctttttgcca tttaagcccg
     3181 ttgatcgtca acacttcatt tggatcgata gtgccgttgt cgagaccgat tgctgctgta
     3241 atcattttaa acgtagatcc aggagcataa cctgtcgcaa atcggctgat gaatggatgt
     3301 tcaggatttt cttcataagc tttgtaatct tcttgtgaga tcccgtttgt cattttgttt
     3361 ggatcatagc ttggagagct agcaagcgca agaagatcac cggtttttgg cgttgtcgca
     3421 acagttgatg cagcttttcc tcctagactg tcaaaagcta ttttttgtgc cttcgcatcg
     3481 attgtcaatt tgatatcttt gccattttga acttcatgtt cgatcagaac ttttttctct
     3541 acaccgtctg catctgtaat gcttaatttc ccacctgttg tcccacgaag atccttatca
     3601 aaagccatct ccaaacctga gcgaccgatt tttccattac tgcttaattc tggattctta
     3661 tcaatatctt ctgctgtgat atctcccacg taaccaatca gctgagcagc tgcttcaccc
     3721 aaaggataat atctgccgtc tacttcttga atggtagctc cagctggaag ttctggcgtt
     3781 gctccatcaa tgattttcaa tgggacaaag taatcgggtt gtacccagct ttgtgaaatc
     3841 gcctgattga tagcatcttc tgttaagtcg aaggaggaag caatcgcttt gatattggct
     3901 gttttttcgc ctccatctcc aagtttactt ggaacgactc ctaattgttt tagtttgcct
     3961 gttgttgcta atggttcacc atttcgatct ataatattcc ctcttgctgc ttcttgtgtg
     4021 gtcaggctta ctttgtcatt tccttccatt tctggaaaaa ccaagttggg ttgccagttg
     4081 atcgtggttt gaccatcatt tctgtctaat gttcctttat aagaaagatc tttcaattca
     4141 cctaatgagg tattcatctt tgctttatat gaaaaactgt aagtagaatc gtctttttta
     4201 tctactttta gattcgatat ctgaagtcct ttgacatcag cagcaccgta tatattctgg
     4261 tatttatcta agatctcttt ttcagataat gcacttttat ttgccgcctt ttttgatgtc
     4321 atttctgcag ctttgttata atctcccttg ttcaaagctt ggacaaattg ctcaactgtc
     4381 ttttctccag cttctactgc ttgggtttct tgatagtgct ggtagtaaaa ataaccgcca
     4441 cccgcagctg ctattaagat cactgcgccg gcaatataag cgcctgttcg atttttgccg
     4501 tgcttgtcac ttcttttcat tttttcctcc ttctcttgct tgtcattcct ttatttcact
     4561 atttatacct gtttattcta acatatgtct ttgaggaaat ccataccaca atccagaagc
     4621 acactttctg gaaggttcgt ttggatacta ccaagtaatt tcccaaccgc tttgggtagc
     4681 ttgaatattg aagttacgtc tttcagctgc tgagagatag ctttgatcac ttgctgctgc
     4741 cttctgatcc tcccgaagtc tccttctttg tcttcccgaa atcgagcata ctggagtaaa
     4801 gtattaccat ccatcacttg ctctcctttg tttatatata cgccatctaa atctagagat
     4861 ttttctgcat cgatcttgac tccaccagga aacagttcat taacgcaatc gataaaatct
     4921 tgaaatgtga tacttgcata atagggagct tcaaatttga aattttcctt taatgtttga
     4981 tttaataaat caactccccc ataagagtaa gctgcattta tcttattcat tccgtaacct
     5041 ggaatatcga catatgaatc tctcatgata gagatcagtt ttgcttgttg tgttttctgg
     5101 ttgtattgtg caaccagaag tgaatccgag cgacctccac caatcgatct tttttcaccg
     5161 catctcctcc tcacacagca atccacttgt caggtgagga gcgagtttgt ctcatcaaat
     5221 aagtcagtca caaaaatata aagaaaaggt cagaaacctt ttcgaacaag aattctgacc
     5281 tagcatcatc tcatcgtccg agttttagca ctatacaacg taaagaaaac attcttagct
     5341 attggaaaaa gccttaatcc aacagtttct gttttaccca ttggcgactt tcgacgtttc
     5401 tgggcaacag cctatgtttg tataggagcc tcacctaaca aggttattac tatattctat
     5461 ttacgagttc atgatagact tttcctaatg attaggcaat ggaatttgaa atgaaatagt
     5521 gaaatttaac aatctttaat attttttaga aaaaaataac aaataagttg aaagaatccc
     5581 gttgcaaatt ctttcagttg caccaggatt ctttgataat tttttagttt tatgtgtttt
     5641 tttatatgtt ttttcacttt tcggaataaa tatttatttg ttaaagtaat taaacaatcc
     5701 gctaatcgtt tgatctggca tttctatttc cttaccatct aaagtaacag tagtaacgac
     5761 aaaatggtct tgcaatggcg gaacttctaa gtactgccct tgcagaaaat cattcactgt
     5821 ttcatattct tttttcattt cttttgaatc tgtcgatggt ttatagctta tttttaacat
     5881 aactagtgct cctttttatt cgggacgtgc aatcgtgaaa atttctccta ttgctgcata
     5941 atcatttcct gccaaacggc ttacagcatt caattttctt ggatcgatcc ttcctgtctt
     6001 atcatcatag actgtttcat ttaaatgata tttctgtact tctagcaaaa ataaatcagc
     6061 tgttacttta tttttattcc gtatctcgac atgttcatat aaggatgttt caaaacgaac
     6121 ttttgcttct gtcaatgctg gtactgaaat gattactgaa tcagttaaag tcaagtcagt
     6181 caggtacagt tcgctttcct cattaggcag attggctgct gttttattta cttcttctac
     6241 gttgtcctca tcaaggatat ggataacagc ttctcttgtc tcaagaatat tctttgctgt
     6301 gtcttttact gcatcttgtt ttctctggat cgccaaggat aagatgggtc gattcgaact
     6361 gacgatattg aagtaactga agggtgcgat attcactata tcttttgctg atttagtcgt
     6421 tactactgct actggtctag ggatgactga accaatcagc aacttgtaat tttcacgttc
     6481 tgtcaattct gaaggactcc gttcaaacat ggtttcgcct tctttctata aaattcgtta
     6541 aaaagcagat cttgaagaca aaatgaaaag taatgctttt ttcatcttct tagaatctgc
     6601 tttaaactat ctatcaattt ttcttttttg tcaagaatct gtgccgtgcg tgttttttag
     6661 ttatttgctt tacttgcggc ttcgacatat cttttagtga tcaattgcgg acgggtgata
     6721 atagatccaa ctacgactga aaaagcacct aattcatatg ctttctctac ttgttcagga
     6781 gagtcagtat gtccttccat aattactggg atatcgactt gttctatgat ttgattcaat
     6841 aaatcaaaat caggtgtctg cgtctttcgt gaatattctg tatagcctgc taaagtcgta
     6901 gacaaaagat caaaaccatc ttcttgcgct tgaagcgctt cctcaaaagt cgaaatatct
     6961 gccatagcta accgaccgtg ttcgtgaatc atttggacaa gttcagcagt tgttttttct
     7021 gtcggacgtt tacgagaagt tgcatccagt gcaatgattt ctgcttgcgt ctccaaaagt
     7081 tcttccactt ctttttctgt cgcagtaata aacacttctg acccatcgta atctcttttg
     7141 ataatcccta ttaccggtaa atcaacgatt cgcttgattt cattgatatc ttctgtcgta
     7201 tttgctcgaa taccaactgc tccgccttct tttgcagcca aagccattct ccccataata
     7261 aaagaactgt gtagcggctc gttttccaaa gcttgacaag aaacgacgac tccgcctttg
     7321 atttgttcca gtgtatccac aatatctcct ccagtaatgt tatataaaaa tacagcttca
     7381 tataaaaatg taaccctttt ctattcagga gacaataatg agctatttct ttcagcagac
     7441 ctttcttact aaaagaaaat tacttcaaat tcttcgtata cgaccagctt ctcttctgta
     7501 aattctatgt tcaacgcttc tttataagca tgtttaaaaa actcagcatg gactttccgc
     7561 caataagcta atgtccgatc cccttctcct tctttccaag catgctcttt cgtgacttca
     7621 ctaaaaggca agatggtcac atttatattt ttgatgatac aaactggttc attttttcca
     7681 ttcaggacca cgtctaattg accaactttt ggtaaaggct cattctcttg tttatacaga
     7741 tcgtaaccac ttgatgtacc agtctttatc ttttttacaa ctagttcgct caactcgtct
     7801 gccatttttt ctgtatcccc aaatgaaaag aaacctaatt tttctgattg ataactagga
     7861 tatttttctt ggaacttttt gacatattct tctacttgct gatacataac atccacccct
     7921 ttgcttttta taatgaaaaa accatccaga tactagtata aacaattatc tggatggtga
     7981 gcataatgaa ttattgaata ttcaagacac gcagactatc ttcaacgatt tgttttggtg
     8041 ttaaatgata acgttcataa agctgcccta caggaacgct atccgtgaat tctttctctg
     8101 caccaaaatt taatacgttc attgaagaat caccataata acgactgata ttttcgccaa
     8161 atcctcctga caaactacca tcttctaatg tgatgactaa ttcgtgtgtt tcttttaatt
     8221 tatctaatgt ttctacgtca ttttctgtga tcgttcctgg attgatcaac gttgcttcga
     8281 ttcctttttt tctaaaagct tttactgttt cttttcctaa ttcaagaaaa ccgcctaaag
     8341 ctaaaattgc tacttttcct ccattattta ctgtttgata ggctggaata ctataatcat
     8401 ttgcgatagc tgtaccatgt tctacaccat gtgtaggaat tcggattgct acaggctgcg
     8461 ctgtttgttc gaacgcccaa tctagcatag agatcatatc ctcttttgtt gctggagcaa
     8521 gatatttgat gttcgggata cttgataagt aaggaatgtc aaatgttcct tggtgtgttc
     8581 ggtcactagc tgagatcgtt ccgccaccga ctaaaataat cactggattc tcgttgatcg
     8641 ctaaatcatg tgataattga tcatatgccc gttgcaagaa agtacttgat tggaagacga
     8701 ctggacgtac tccctgagaa gccaaagcag aagcgaaagt gatagagtgc tgttcagcga
     8761 tccccacgtc aaaataatga tctggatgtt tttcttggaa tgttttcagt ccaaaggcgc
     8821 ctggtatcgc tgcattgact gcggcaatag gagctgtact tgtttgcatt ttcttatcca
     8881 aataatccat gatgatcata ttaaatgttt ctccagaagt tacttgcttt gcctccccgg
     8941 tggacagatc aaatggtaca tgccaatgat atttcatttt attatcgatt gccggctgat
     9001 aaccgcgacc tttttccgta tggacgtgaa gtacaatcgg atgatcaata tcttttactt
     9061 cttcaaataa acggatcacc gtttccagat cgtttccatt atccacgtaa cgataatcaa
     9121 atcccattgc tttaaatagg ttattgctgc ttttaccgtt ggtttgacga agctcagcta
     9181 agtttttata taaactgcca tgattctcat caatagacat ctcattatcg tttactaata
     9241 cgattagatt ggattgtaaa acaccaccat tgttcaatcc ttcaaaagct aagccaccac
     9301 ttagggagcc gtcaccgata aatgctacca catttcctga ttctttcttc agatctctcg
     9361 ctttagctaa acctgttgct aagctgatag atgtagatgt gtgccccact gtaaaatagt
     9421 catgtttact ttcatgttgg ttggaatatc cgctcacttc acggtaatgt cctttttcaa
     9481 aaccattttt tcgaccagtt aaaagcttat gaccataaga ctgatgggaa acatcccaaa
     9541 cgattttatc ctttggtgaa tcaaatacat aatgaaacgc aatcgttaat tctactacac
     9601 ctagattcgg accaacgtgc ccacctacat cgctgtcctt ttgcaagatt ttttggcgca
     9661 tctcttctgc caactgtttc atttcctcaa cagataattt tttgacatct gcggggctat
     9721 tgattgcatc caatacatgt aatactgtac tcataataca ttctctcctc cgtactatct
     9781 tcttttaatg aaaatagatt tgttacactt tatctattct actacttaga gttgactcta
     9841 agtcaatagc attttaaaaa aatttcagag aaagcttgtt atataagtag atcttcgcac
     9901 gaatcaggac tcaaataaaa accttctaca gacaattaaa aagctttgta gaaggtctcc
     9961 taattaaggt ctcatcaaga aatatgtttt tttaataggt gaagttcctg agcaatatga
    10021 tacaaatgga tgtttgcacc ttttaatgag attggataga tctttgtctt ttcagtttct
    10081 aatccatttt gatagctagc agcatcgcac aaaaccactc tttttttcgc acatagacat
    10141 tcatcaggca attcttctaa tgaacgaaat aaaataggat ttatttcagc gatgccttct
    10201 tccgcataca aatgaaattg ggttcgataa aaaaattgtt tgactgaact ttctgaaaca
    10261 acaaaaattt ctatttcctg tctccccgct tgttcactgt tgatcaaaga aagacttttg
    10321 acgaattttc gaatgaaatt ttcattccat cttagttgga tttgattttt ctttttccac
    10381 tcttccaata tttctacaat ttttccgtac agaagttgtt ctttttctga tagaagatag
    10441 atcctttccg gtaaaaatac ttgtccatct gcccacaacg tacggacaaa ttgtaaaaag
    10501 gattgcttga aaataaaatt ttctgcatcg tcgatttcag cgatactaat aatctgtaaa
    10561 atcaattcat tcagtgatag gtcattgaga acaaaatttt tataaacaac ttcgaaatct
    10621 tttcttaaca gctccatgtt ctcattggta taatttctac tgttgaacaa agaaaaaata
    10681 taatagacat cttcttcttg taagtctaga attccttttt gctcttccaa tccttgacag
    10741 actaattgaa ataacggctt cgtttctgcg cttttcttat cttcttcttt cagtccaagg
    10801 ggaaatgcct ttcttccgat tgcgatctct attcctcgta tgataaagga cttttcatct
    10861 tccgaatatc ttcttttaaa aaaatgagtc tctacataat caatcaactc gttacaagct
    10921 tcttctacat acggattttt attttcatag ccttcccaat tgacataacg tactaaagct
    10981 agcaagatgt tccgcgagtc aaattctggg ataatgattt ctttattttt caaataatca
    11041 ttttctttaa aaaaatccaa tacgattttt ttgattgtat atactttgct caaagaaata
    11101 aattcaagat cagccagttc tgtactcgtg aatataccat taaaataata acttaaaaac
    11161 tttaaaaaat aagattgacc gtacagaaac tgtgtgagct cagaaagagc aataccatgt
    11221 ttgaatacga gtttatactt atgcttctct cttacttgtt cttttataca atattctaga
    11281 cactcggcta gactttctag atcgtgaagg atcgttggtg tagtcaccct tagagaaata
    11341 gctgtttgtt cgatgtctat ttcttggaat tcaaataatt gttcacacag atacactttt
    11401 ctaaatatat ttcgctcgat gtaattttcc agcattcctg atacacgcct ccagtttttc
    11461 ttatgatttt atttaatcat atctgtaaat cgtatttttg tttccttttt atttagaaaa
    11521 gccctttcaa aaaccaataa aaaaagaaac tgggacaaaa gtcaaacaag ctcacaattt
    11581 cagcctcatt gtaggtagct ttgagccgtt tggactttta ccccagcctc tttcaagaat
    11641 ggttattcaa agatgtcagc gtctagttct ttgactacat ctttcaacaa ttggatacca
    11701 gctgcgctgt taccaaatgg atctagtgcc gggctgaaga cacctatgcc gtaacgattt
    11761 ggcacagctg ccatcaaccc gccgcctaca ccgctttttg caggtacacc aacatggacg
    11821 gaaaactctc ctgattcatc atacaaacca gcagttgtca taattgattt gacgattgta
    11881 gcactttctt cagtaatgat ttgtttgcca ttccaaggag caattccttt atttgccagc
    11941 acagcagcta attttgctat gcctgtagcg gtgacattga gggagcattg tctgaaataa
    12001 gcatcgagta tctccggtac gtctccttcg atcatttgat tacctttcat atagtaagca
    12061 agagagcgat tgatatcacc tgttcttgat tcagaatgat agatttcttc attcaatgtg
    12121 atattaggat catcacagat ctctttcata aatgacaaga ttcgctcaaa tttttcatct
    12181 gcatctttac cggcaatcaa cgaggttgtt gcaatcgctc ctgcattaac aaaaggattc
    12241 attggatgtt tgcgatgatt gatttccata ttcatgatag aattgaaggc aaaaccggtt
    12301 ggttccgtgt tgattgtttg aaagactttt tctattccgt tgtctttgat tgctaataac
    12361 aatacgggaa cttttgacat gctttcgatg gcaaagcgca catcggcgtc tcctccctca
    12421 atcagttgtt ttgtatccaa atcataaatc gccataccta attgtttggg atccacttca
    12481 gctaatgctg gaatgtaatc cgccacttgt cctgtttgat aatagtggaa attttttcca
    12541 atcaattgtt ctaattcttt tacttccatt tgaataacca gctttcattt tttatttttc
    12601 tgcatgctgg aatttatgac ggcatgcgta gatcgtaaat ggaataacta agacgataag
    12661 ataagaaaca atcagaatcg tttcataaat cccatgctgt ccagctgcga tactagcagg
    12721 tggaacaaat gagatacaaa aagcaaaaag tgaagtcaaa agacctaaac aagcaacgat
    12781 tacttttaca acttttccgc ctggaacctg ataggctctt tcaagactgt catctttaaa
    12841 aaccagcttc aaatagcccg cgaaaaacat aaagtacaac atcaggtaga ttacaacagt
    12901 caatgacatc gctgcaagaa atgaaagatt attccccccg ccgcttaatg taaagacggc
    12961 tgcccagatt gttgcaacga tcccatttgc gatcatcaaa tgagcaggaa caccgtgtgt
    13021 gttttctttt gcaaaatatt caggaagtaa tccttcttct gctgctacgt gaagtgcttt
    13081 tgaaggagag gttacccaag aagcaatttc tccgatgaca cctaaacaaa caagaatcgc
    13141 aagaattttc gcaatccatg caagatttgg atcaatgtgt cttaatagga attctaacga
    13201 ttgaatcacc cctgtattaa gagacaaacc atgttgaggt actgtagtag cgacggtcaa
    13261 tccgccaaaa gtatctaaaa tgattgccag aataaccaat acaaacattg ctaatggata
    13321 atccttttta gggttttcca tttcatttgc atgactggca gatgtttcta cgcccatata
    13381 tgctaaaata aaggaaacaa aaacaaccag cgtaggcaat ttcgaaaaat caggaatcaa
    13441 tgttttcata gacagtatcg tttgtagtgg agcgcctgaa gcgacataat ggatccctaa
    13501 gccaaacaag actaatgctg gcaacaagat accagctaca aaaccgatcc gagcaatcaa
    13561 tgaagtgtat ttggttcctt ttaactgtga gagtaaaata gcccagaaaa taatcaatag
    13621 gattgccaat ttcagatatt tattgttttg tatagcacca atacccgttg cataggataa
    13681 tgcaccagtt aagaaataca gcatggtcaa gtagccaact gttatctcaa accattggaa
    13741 aaagattgcg gcaaaaccga atcgttttcc caatgtgcga ctgacccaag tatagacacc
    13801 gcctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
    13861 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
    13921 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaacgt
    13981 taattttttt gcaggaattt tcgttttgct ttgttgcatg aatcttcctc ttttctattt
    14041 gaatttgttt caattgagtt tgttgccaaa gaagaaaaag tccaatcctt cttcttgttc
    14101 ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat tttaaaatca ctgttgtcag
    14161 cacataggca tacacggcag taatgatgac accaatcact tgaatcccta agaagtacca
    14221 gctgcttgga ttcgttagta aatggctgtt agatgcaaag atccctatca gaatcgtacc
    14281 tgtgaatccg cccatgccat gaacacccca cacatcaagt gcatcatccc attgtttctt
    14341 tttacgaaaa tcaacagcag catgacagac gatacccgca atgatcccaa tcagcatggc
    14401 actttttgct tctacataac cggcacaagg cgtaatggct gccaatccag caacggatcc
    14461 tgttagtacg tcgacaaaat caaagccatt tccttttact tttgcaatga tcagccaagt
    14521 aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat gcattgactg cttgttcatc
    14581 tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca aaccaaagca gtcctgtacc
    14641 gatagcagct gccataagac tgctatgctg acttctttct cctaaaggaa tctttctttg
    14701 accaagtgtg aggatacatg ctaaagagcc gaatccagct gtcgtgtgga tgactgctcc
    14761 accagcgaaa tctctaaatc ccatctgatc caaaaatccg ccaccccata tccagtggca
    14821 aacagggaaa taaatcagca gattccaaaa aataacaagt ataatatagc cttttaaatt
    14881 caatctccct gcaaaagctc ctgtcatcaa aggaacggta atcacgataa acatcagttg
    14941 gtaaagaaag aacattaaaa atggaatcgt tgcaccatga aacatgttgg ggaagcttgt
    15001 aacatgtctc atcaaaaaga aatctgccgg gtttccaata acaccgccga tatcccgtcc
    15061 aaaggccaat ccgaatccac caaataccca cataatcgtt gttatcccaa cggagagaaa
    15121 agattgagac atgatcgtta gtatattatt cttccgtgct aatccgccgt aaaaaaaagc
    15181 tagaccagga gtcatcaggc aaaccattgc tgtacataaa atcatgaatg ctgtatctcc
    15241 tgtattgata ggtaatgtat tcattttttt cgcctccttt ctacgattac gagtataaaa
    15301 gaaggtgtag ttgttttttt cttctgtttt tttgaattaa tacttgcatt tataaaagac
    15361 agacaattaa taaaagcttg atgtgttgtg ctctttttcg tttttcacac aaataaagac
    15421 aaaaaataaa cggtcttctt tttatagaaa accgtttatt ttcgtctttt tttattttta
    15481 aagatatttt ttcataagga ataaa
//
LOCUS       DANJJH010000069        15239 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000069 DANJJH010000000
VERSION     DANJJH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS53_002009"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS53_002009"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL0872220.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS53_002010"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS53_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL0872221.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS53_002011"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS53_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL0872222.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS53_002012"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS53_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL0872223.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS53_002013"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS53_002013"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL0872224.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS53_002014"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS53_002014"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL0872225.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PWS53_002015"
     CDS             complement(8053..8523)
                     /locus_tag="PWS53_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL0872226.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PWS53_002016"
     CDS             complement(8569..8994)
                     /locus_tag="PWS53_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL0872227.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PWS53_002017"
     CDS             complement(9061..9330)
                     /locus_tag="PWS53_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL0872228.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PWS53_002018"
     CDS             complement(9341..10936)
                     /locus_tag="PWS53_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0872229.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS53_002019"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS53_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL0872230.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PWS53_002020"
     CDS             complement(14489..15049)
                     /locus_tag="PWS53_002020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL0872231.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3954 a         3442 c         2699 g         5144 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaa
//
LOCUS       DANJJH010000070        15072 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000070 DANJJH010000000
VERSION     DANJJH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..170
                     /locus_tag="PWS53_002021"
     CDS             <1..170
                     /locus_tag="PWS53_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0872232.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="PWS53_002022"
     CDS             complement(553..1281)
                     /locus_tag="PWS53_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL0872233.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="PWS53_002023"
     CDS             complement(1259..2833)
                     /locus_tag="PWS53_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0872234.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="PWS53_002024"
     CDS             3062..4000
                     /locus_tag="PWS53_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0872235.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="PWS53_002025"
     CDS             4012..4923
                     /locus_tag="PWS53_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0872236.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="PWS53_002026"
     CDS             4947..6419
                     /locus_tag="PWS53_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0872237.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="PWS53_002027"
     CDS             6470..7081
                     /locus_tag="PWS53_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0872238.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="PWS53_002028"
     CDS             7111..9024
                     /locus_tag="PWS53_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL0872239.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="PWS53_002029"
     CDS             9152..12133
                     /locus_tag="PWS53_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL0872240.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="PWS53_002030"
     CDS             12130..13791
                     /locus_tag="PWS53_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL0872241.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="PWS53_002031"
     CDS             13831..14763
                     /locus_tag="PWS53_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL0872242.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DANJJH010000071        14396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000071 DANJJH010000000
VERSION     DANJJH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            292..624
                     /locus_tag="PWS53_002032"
     CDS             292..624
                     /locus_tag="PWS53_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0872243.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            920..1075
                     /locus_tag="PWS53_002033"
     CDS             920..1075
                     /locus_tag="PWS53_002033"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL0872244.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1351..2691
                     /locus_tag="PWS53_002034"
                     /pseudo
     CDS             1351..2691
                     /locus_tag="PWS53_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2932..4548)
                     /locus_tag="PWS53_002035"
     CDS             complement(2932..4548)
                     /locus_tag="PWS53_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0872245.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4666..4884
                     /locus_tag="PWS53_002036"
     CDS             4666..4884
                     /locus_tag="PWS53_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872246.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5079..5540
                     /locus_tag="PWS53_002037"
     CDS             5079..5540
                     /locus_tag="PWS53_002037"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0872247.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5733..5972)
                     /locus_tag="PWS53_002038"
     CDS             complement(5733..5972)
                     /locus_tag="PWS53_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872248.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(5996..7519)
                     /locus_tag="PWS53_002039"
     CDS             complement(5996..7519)
                     /locus_tag="PWS53_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0872249.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7537..7659)
                     /locus_tag="PWS53_002040"
     CDS             complement(7537..7659)
                     /locus_tag="PWS53_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0872250.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7689..8672)
                     /locus_tag="PWS53_002041"
     CDS             complement(7689..8672)
                     /locus_tag="PWS53_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0872251.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8696..8845)
                     /gene="rpmG"
                     /locus_tag="PWS53_002042"
     CDS             complement(8696..8845)
                     /gene="rpmG"
                     /locus_tag="PWS53_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0872252.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8866..9243)
                     /locus_tag="PWS53_002043"
     CDS             complement(8866..9243)
                     /locus_tag="PWS53_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872253.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9275..9454)
                     /gene="rpmF"
                     /locus_tag="PWS53_002044"
     CDS             complement(9275..9454)
                     /gene="rpmF"
                     /locus_tag="PWS53_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0872254.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9469..9738)
                     /gene="rpsN"
                     /locus_tag="PWS53_002045"
     CDS             complement(9469..9738)
                     /gene="rpsN"
                     /locus_tag="PWS53_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0872255.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10261..12027)
                     /locus_tag="PWS53_002046"
     CDS             complement(10261..12027)
                     /locus_tag="PWS53_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872256.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(11987..13740)
                     /locus_tag="PWS53_002047"
                     /pseudo
     CDS             complement(11987..13740)
                     /locus_tag="PWS53_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(13850..>14396)
                     /locus_tag="PWS53_002048"
     CDS             complement(13850..>14396)
                     /locus_tag="PWS53_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL0872257.1"
                     /translation="IGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVY
                     ISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQ
                     VASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLV
                     VASLSMIGVLIGRKVLKKYKK"
BASE COUNT         4598 a         2807 c         2208 g         4783 t
ORIGIN      
        1 cgtatgaagt gggcaggagg tcgaatccag aactgggcta tggtacttca acgaggactt
       61 ctcctctaga attgaaaagc atgcgattta ccttccgtaa cgacggtaga tttattcaac
      121 tatgtgtcca atcaaaattt gtctgatttc aaaaatagtc aagagaattt tggttgttaa
      181 aaactcttat ctatcaactt ccacagttta gttgacaaac cctgatacta agttgggcta
      241 tgatacactt ttcttgacta tcagttacgt taccggaagg aatgaactct attgagaaaa
      301 acatatgatc gtaaatttaa actaaagatt tcacaggata tccttgagaa aaaagtaact
      361 accaagaaaa ttgctgaaga atacaatatc tctcgtccaa caatttcccg atgggtttca
      421 gaatatcgtc gatacgggaa aaatgctttt gctggacaag gaaaaagatt acctgataaa
      481 gcagatttct acattcttga acaggaaaat aaacgactaa aagaggaaga cgagtgttct
      541 acgaacacaa agatcgatac ggatctccag ggatcactca aaaactttac gatgaaggaa
      601 tcgaaatcaa taaacgtgtt gtagctgttc ttatgcgtga aatgaattta tgcacaagag
      661 gatttcatcg acgcaaatct tctcatggta gagaaaaaac aatcaaagaa atggtaaaaa
      721 aatgttgaat cgataatttg aacagaacca aattgatgct gtttgggtga tggatattac
      781 ttacattcca tgtaatgacg gtcgcctcta tttgtccacc tatattgact tagcaactcg
      841 tattctacgt tactatatgg tagattcaca catgaaaaaa gagattgtcg tccgtccatt
      901 acaaatttat taaggagaat tgcctacggt aattcattcc gatagaggta gccagcatcg
      961 gtcttttatt tatcaagagc tgttgaacga ccaccacatc acacattcag tgagccaaac
     1021 tgggactcct gttgacaatg cagtaattga agcgtttcat cgatcaatca aatgagaatt
     1081 aatcgactcc aacaaatata aaagtaaaat agagatgaag gtgttaatcc aagattattt
     1141 agaagactac tacccaaaca aaagaatcca tacaaaattt atgatgactc cttgacaatt
     1201 tgaagcgaag aaaataaggg atctataata tctagtgttg gtgacgcctt ctgttgccaa
     1261 tactttttct tttttctaat aaacgcaaac agacacctta aaatccttta gaataaagtc
     1321 gacgaaaacc attcaaagga gtgtttcaag atgtcctatt cagagtttac caaagagtta
     1381 ctagatattc tagacctaaa tcttatcttt catgaagatg cctttcgcaa agagcgaatc
     1441 aatgacgaaa catgttttgt ttttgatggc acattgacct atcaaccgga ggaatgcttt
     1501 catcgccact atcaaaatga acaaacgatc attaaatagg gctggaaaaa agtatccatc
     1561 ttattgaatg atgtcagtaa ctacaagacc attctgcgga ttaacaaaca gcgcttcaaa
     1621 tgtaaacact gtggaaagac ttttttagca gaagattcag ttagtgatcg tcgctgctcc
     1681 attgctcgca gagtcaaaca agccattctt gagctattaa gcgaacctct ttctatgtct
     1741 ttgatcgcac gtatgaagca catttctccg accactgtca ttcgaatact tcgcagcctt
     1801 cggcctaaaa ccatttcttt gagccagctg ttaccagaag tggtgtgttt cgatgaattc
     1861 aaatcagtga aaaatgtctc gggagccatg agctttgtca tgatggacgg caagacccat
     1921 caactgattg atattgtgga aaatcggcaa ctcaatcctt tacgcgacta tttcttgcgc
     1981 taccctataa aagtccgtga acaagtgcgt ctagtggttt cagatttcta tacaccctat
     2041 cgcacattgg tgaaagagtg ttttccgaat gcccgaatcg tcgccgatcg ctttcatatt
     2101 agccaacaca ttggtcgggc ttttaccaac caccgaattc aagtaatgaa gacctttaaa
     2161 aaaggggatc gtcggtcgaa gcacttaaag aaatactgga aactacttca aaagaatgcg
     2221 tgggaattga aaggtcagca tcgctactgg cgtccgtcat ttcgagatca tctgacagaa
     2281 gcagagatag tcgatcggct attatcttat gatgattcct taaagcgagg ctacgaagtc
     2341 taccaggatt ttctttccgt tatcagaaga caagatgttc ctgaattcgt cgcgctccta
     2401 aaagaagact acaaggagtt gccagagcac taccaaccag tgttcacgac cttcaagaaa
     2461 taccaaacag aaatcaaacg agcgttgcgc gttccctatt ccaacggccc tatagaatgt
     2521 ttgaataacc atatcaaagt actaaaacga attgcctatg gctatagaaa ctttcaaaac
     2581 taccgagaac gaatcttttt atatcgagga aaatatttta agaaaactaa gaatactacc
     2641 caactgacta aagccagaac aacaacacgg ctagacaaga tagctgtcta gccgtgtgag
     2701 tatctcgatt attctattga atttcttcac caacactcat tgacgaagag ccaaaataag
     2761 taatctaacc gactgttaac taaaccattg caggtcccgt taaagatact tgtgcctgtt
     2821 gtcaccgctt ctttttcttg tccacaagcg cttaacacac ttaataaagc ccctgctatt
     2881 attaataata atatcttttt cttattaaaa acctcctcat ctatttccag attagtaaat
     2941 gtagtaatct gtgctttctt ttttctcaat actaataact aacttatggg gtaaacaaat
     3001 actcgtttga ccgggttttg agatccatcc agttctaacc gcgatttgat caggactatt
     3061 atcttcttta actcgaattc ttccatcttt aatctcaaca atattatatt gtcccttcgc
     3121 aggatgataa ttcacgagtt tatgattgat cttatccaaa tcgaagcgat ccatttcctt
     3181 gccatcaatt cgaacgatgg catatttgat agcatcttca gttgtttcat tctccttttc
     3241 tatagagaat acaaataagg gaataaaaga ggtaataatc aaaatgccaa taattatgac
     3301 atcgcctatc ttgacaccag cttttcttaa aaatttcatt aacgatatct ggctaaacat
     3361 attagacttt ttccatggaa atggtgaaat ttttcggaaa ttatctgcat taagagaatc
     3421 agttaaacaa ccctcaagtt gttccttaat acattgttga tccatcttca taccaataaa
     3481 aactagttta ttaatgcgtt caccaaacaa cggatgccag gcttccttca attcgggatt
     3541 tttttgtata atcatttgac actcaatttt aggtaaacta gctacccaat aatttaatga
     3601 ttcaatactt ttatagctac ccgatgtttc aaagatcgtc gaaaaatcac tatgttgggc
     3661 aaaccaaaga aacccttttg ctcttgtaat attgccagga aaatttttat ccgtaagatg
     3721 ggcaaatttt tgtgcgtcaa ggagtaaccg atttcgatag ataaaacttg agataccata
     3781 ttcttctgtt tcaggaatat gattttgata gccaagctct aactctttga accagcccac
     3841 tgaataggaa gtctcatcta tattaaaaag atttgtattt agaattttcg ataagtctac
     3901 ttcaccttta attgtcctaa ttattttggc attggactgt aacttagcaa ttagaccgtt
     3961 aattttgtaa atcgtcttgg ttgacactaa gtcactatta tttaaaagta atatgttaca
     4021 acactcgaat tgatcaatta acaagccacg aatatcctgt tcttcttcaa ctacattacc
     4081 ttcagctttt ctttcctgaa tcttttcacc cgtttcgaaa ttatcccaaa aacgttttac
     4141 atccactact ataataatgg caccaatctc taatcgttgg gtcaaatcaa tgtctaaagg
     4201 gtttttagct aatataattg cttgaataat tggaattggt ttgctgattc ctgaagcttc
     4261 aataacaatt tgattcaagt cagaaaattg cactaattga tctagttcaa caatcaaatc
     4321 ttcgcgtaat gtacaacaaa tgcatccatt cggcatcgaa acaagttttt ctccagttct
     4381 tttaaaacct ttcttgtcga ctaatacccc atcaacattt acttccccta tgtcatttac
     4441 aatgacagca atttcctttc cgtgatcacc gtacagaata tgatttagaa gtatcgtttt
     4501 tcctgaacca agatagccag ttaagattgt aataggtatt tttttcatag ggaacctctt
     4561 tctatacctt gaatttacac tttaagtgca acacataaaa atgagtcata atcgatatcc
     4621 aactagtata agtttgtcga gaacactcct agaaggaaaa taattatgag ccagtatcgt
     4681 catcttacta tactcgaacg agaaagaatc tttctgcttc acgaagaagg attttctatt
     4741 agtataattg gcaacgacat tcagcatagc ccgtttacta ttttacatga actgagaaga
     4801 aacaagaaaa aaactattca ccttccaaag cggaaaaaaa ttatgcttat cgaagaaaaa
     4861 aatgcggacg ttatcacatt ctaaatgatc cgtgagtttg gtcaattgtt cgtcatttat
     4921 ttgtcgattt tcagtggtcg ccagaggaaa tctcgaatcg tttgaagcat gaacatatgg
     4981 acttttcaat tagttttatc acgatctaac gtgcaatttg caatgatctt ttcgatacag
     5041 aacccctctc acacggcaat aaaggcctga tccgttcatt gcaccatcga ggaaaaacaa
     5101 gacatacaaa aaatcatgtc gagtctcgtg gaaagatttg ccttactcat tcgatccatg
     5161 gccgtccaaa atccgcagat gatagaagtg attttggtca ttggaaagcc gatattgtag
     5221 ctggcaaaac tggtggtact tgtttagtga ctttgacaga cagaaaatca tgcttccttt
     5281 tagcaggaaa gaaggtcccc agaaagaaag ccgcttatgt gagcgagaaa atgattgaac
     5341 ttttaagcag tattccaaat gaggcggtca aaacgatcac tccagataga ggaaaagaat
     5401 tctccaatca tttcgcagtt acagaagcat tgaataatat gcccttttat tttccagatc
     5461 ctcatgccct tggcaacaat gaacaaatta aaatacgaat agactcattc gagaatactt
     5521 gccaaaatcg aaagagatag ctacggtaga taactcaacg ataaatcaga tgattgaaaa
     5581 actgaataac agacctagaa agtgtttaga ttggagaaca ccttatgaag tcttctttga
     5641 taaagtgttg cgcttgattt gacaattcaa gacataataa aaagcagatc aaatccaaat
     5701 aagggaaaaa tgatctactc tggtattatc aattaattgg ccaagctatt atgccgtttt
     5761 gctaaacgtc tacttcttcc agtattgacc ttgataaaac aacaatcagg atcccgtttt
     5821 aatactctat gaactgtgtc aatactaaca tgtaaagttg cagcaatatc agctaatgta
     5881 ctcccattta atctcatttg ctttgcaaat cgataacgcc gataagcatc aatattcttg
     5941 gaatccatag acaataatac ttttcttacc atgtatttcc tccaaaaatt gaattttaat
     6001 gagccatcat ttcttgagca atttcatgtt tagacaatga agctggatag aaagtaggcc
     6061 aattatgcaa ttcattatag agtttttctt gactttctcc accaaagaaa atatggaaat
     6121 gctctgcctt acttggtgca attccatgat cactaaattg aatatattta aaacgaccag
     6181 cacctgcatc atcagtttca aaattaaaac gaacaccacg gtttcctttt tcataattca
     6241 aaattttgta tccaacatat ttgtatgtgt attgatgatg ttcaccgttg actacaaaat
     6301 caatggtgtt gtctttaatg ttgatagtat caatatctgt cttatagcca gtagtataat
     6361 aatctttgta ctcggcagct gtcatgtctt tattgatttt tgctttgtaa tcgaaaactt
     6421 catccaaaat tccatctttt aacaacggat aaacagattg ccattcgcct gtataatcac
     6481 tcaattcgcg atccttcaca tcggcatctt taaagtaccc attagcaact gtcttttcat
     6541 ccttcgcttc tttttctgga actacctctt caccagcaac catagttgtt ttttctaacg
     6601 cttttagatt gtcttccatt actgaaacat aatcttcacc attatccatt tgttcttttg
     6661 taagactttc aagaggattc agtacttcca attttatccc agcttcgttg gccaaagttt
     6721 ttgcaatttt atcattagca ttcttttcaa agtaaatata attgatctta ttttttttaa
     6781 catactcttt taattcagct aatcgaccag aagatggctc ttcttcagga cttagtccag
     6841 caataggaac ttgtttcaat ccataatcca aagctaaata tccaaaggct gcgtgttgcg
     6901 tcacaaaact tttttgtttc gcttctttaa gactagtggt atagtcagca tccaaccgtt
     6961 tcagtttagt caaatacttt tcagcatttg tctcaaatac ttttgctttt ttcggataca
     7021 atttaactaa ctgatccttg atatttgaaa cttccttaat tgccattttg ggtgaaaccc
     7081 acgtatgagg atctagttca tgatggtgat cttcttctcc gtgatcatga tcttcttctt
     7141 cactacctgg caataaaatc atgtcttttg ttccttctac tacattaggc ccagctttct
     7201 tccaactttc tttagctttt ggtacccatg attccatgtt ttcgttatga tatacaaaaa
     7261 catcagcatc atgagctttt gccatatcct tagctgaggg ctcatagtca tgaggttctg
     7321 atcccgctgg aaccattaaa tccacgttcg cttcatcgcc gacgatgttt ttggtgaaat
     7381 catacattgg atagaatgtt gttacaattt ctaattttcc atctttatca ttcgatactt
     7441 ttgcatcatt agtgctggag caagctccca ataatcctag agctgctatc ccaataagtc
     7501 ctacactgat tttcttcatt ctttttcctt ccttaattat gctaatttct tcattttatt
     7561 tcgtttatgt tctttgataa tttttaatgc cctttttcta gttttaatat tcgggctttt
     7621 aagtcgtcga tatgcactag atatatccgt tttctccatt agaaattacc cccaatcact
     7681 acttatcatt atttgctttc cgccagtaat ttatcaaaac tctgtttgat cctttcttca
     7741 tccaacattt ttccgattag aataatagtg gtttggtctt tttgtcgagt ggtgtattga
     7801 aaattaacct gttcgtttac ctcttgcatg gcaactataa aatcctgctc ttgtaaagca
     7861 atcaatcctt taaaacgata caggctccct tggttggtta aaatcagcca gtcaatccat
     7921 tggagtaaaa aatctttttt tatgtcttga tctgaagtta agagaattga tttaaactca
     7981 tgtacttgat ggtgttgatg atagtatttg ccattatagt gattttctga tgaatcactt
     8041 tcaaaaacta agggatgatt aaattttttc aaattataaa aatccttttt ttgaatcgtt
     8101 tcactatgtg tgaacattcg aatatcggat aaagggttaa tacttttgat ttctctctgt
     8161 aattttttta gtgcagataa attcaagttt tgttttctag acacaaaaat gcgatctgcc
     8221 atgacaagtt gcttaagggc ttgtggttct ctttcaataa gttcaaaact gtcactatca
     8281 ataacagtca ataaactatc gatatatact cttcctctta gttgaggcgc tgttaaaagt
     8341 gtttgaatga ttggttgggg atctgctata cctgtagttt caatgatgat ttgtgaaatt
     8401 ggttgttgat gttcttctat tgtttccagt aaagaaatca atattgctaa taaatcactg
     8461 cgaagactgc aacacataca tccattattt agttgtaaca cccgttcctc gctatgaact
     8521 aataatttgt gatcaatccc aacttcacca aactcatttt caattataat aatttcttcc
     8581 ctggggtagg gtgagacttg caaggcttga ttgattagcg ttgtttttcc agcccctaaa
     8641 aacccagaaa cgattgtaac tggtatgccc attttgtcac ctttttacca cttagctatt
     8701 tcacttcttt aaaaattgcc cgtctgcgca attttggcga atactttttt aattctaacc
     8761 gttcagggtt attccgttta tttttgctgg tgagatacag acgttctcca gtttcgacac
     8821 attccaaaat aatattttga cgcataaatc ttcctccttc aatacttatc gatcttcaag
     8881 atatgcttga atttgttgtg catcttcaaa agttaactcg ccagattcca attgtgaaac
     8941 taaattttct ttcatatcaa caggtatctc ccaataacca tcgatacatt gaaataactc
     9001 ctcgtcatac tcttcaaaaa ttggatagat tcttaaaaaa tcatcaaatg ataagccttg
     9061 gtcctcatag taatctttga ctaatccatt ttcaattgcc tgttgggtcg gtaaacccgt
     9121 ttcagtaata attcctctct ctaccatcga acgataaaaa gtgcatttat ctagcgattg
     9181 ctgggtcgtt tcaagataat actttaatcc ttcttcaact aaaggtgggt aaataatttc
     9241 cattttgatt ccctcctaaa agttatatat taaactattt tgaagctcct ttaattactt
     9301 tctttccatt atagtaccct tttaaagata catgatggct acgtctataa tcaccattcg
     9361 cttcgtcaaa tgaaatttct ggttttacta cagtttgatg agttcttctt aaccgtttct
     9421 tcgccttaga tgtttttctt gctggtactg ccatgatatt tcctcctttt accaacttgc
     9481 tttttttact ccaggaatca acccttgatg ggcaagttct ctaaacttaa tacgggacat
     9541 accgaacttt cgcatatacc ctcttggacg accatccgtc tgatcacgaa gcctcagacg
     9601 attcggattt gaatctcttg gcaatttagc gagagcggtg cgatctcctg cggcttttaa
     9661 ttcctgacgt ttatctgcat acttttcaat catcgccatt tgtttctttg ctttagcaat
     9721 tttcgacttt tttgccatat acctttatac tccttttatt ttgatttaga cacctcaaat
     9781 cgtaatcgtt acgttttgac actgatactt taccaacaag agttgctctt gtcaacaata
     9841 aataacatga aataaaactc atatgaatag ataactattt gaaaacgaat ttatcggtac
     9901 atagagataa tcaaatcatt taaaagaata tgcagtttag gctctgaaaa ataagaattc
     9961 acttttatta gtccaatcaa acacaaatac cgttttaagt agcttttcct ctacttaaaa
    10021 cggcaatatg aaagaaacta taacttttta ttcaatcttt tatgtttaac tggtttatcg
    10081 ccaatttttt aaaactttga ttattcctta tattataaga atagacatta agtgaactaa
    10141 ttcaatcata ctgattcttt cgtgtattca aaattagatg ctgtttaata aaattattct
    10201 ttttgaaaaa gaattataac tatatcccaa aaattgtagg ttaatttaaa gcctatttca
    10261 tcatcgtgat tgagcttctt tttcatagac gaatttctca gatagtttag ttttctttat
    10321 gagatttttg tatatttctg accttcgata caactcatta tgagtacctt gatcaactag
    10381 ttttccttga tccatcacta caattttatt taccttttca atagacttca agcgatgcga
    10441 tataattagt actgtcttat cttgtattaa acgatttaaa ctttcctgaa tacgtttttc
    10501 attttccgca tctaaactgg cagtaatttc atctaaaatt aaaattggcg catttttcaa
    10561 aaaagcccgt gctatggata ggcgttgtcg ctctcctcca gaaagtgaac ttccgttttc
    10621 tccgaccaat gtatgataac cgtcaggtag tttttcaata aagtcttcgc agttagctaa
    10681 acgagctgct tttttcactt cctcatctga cgcttgacta tttccaatac gtatattttc
    10741 caaaatactt gtattaaaca aagttacctc ttgaaaaaca atagcgattt tgctaaacaa
    10801 agaagctgga gatacacgtt taatatcata cccatcaatt aaaatacagc cttcatcata
    10861 atcatacagt ctagacacta atttcaagat actagttttc ccggaaccac tagcacctac
    10921 taacgcagtc acttctcctt gtttagccgt aaaagagata tgatctaaaa ttggggtatt
    10981 gttatcatat gagaaagaga catctcgcaa ctcaatgtca aaagatttca gaggactatc
    11041 tgatccctct tggatactgg tttccttcat agctctaatt cgctgtattt taggagctaa
    11101 ataaaatatt tcaagcactg cttctttcat agaatcgaag gaatccttta tttttattgc
    11161 ggctagtaag taaccaacga cgtagagtat agtcacttca cccgttaaca gtaagtgaac
    11221 acctactaga atgactactg ctaaactaac atatgagaaa atagaggata aggccatgac
    11281 tgaaaaagta cttaattcta cctttaaatg aatcctttca ctctcttcca tttttttgaa
    11341 aagtctgtct tgaacttttt tagatagatt aaagctatta atctcttgct gcaattctat
    11401 agtttcttga aattcttccg agttttctct caaagtatcg taatatttag tattggcttt
    11461 cgtttgggat ttctttgata acaagattaa gacaaaacta aataacgttg gcaaaataac
    11521 agctaatccc attttgacat taccaaccaa aagcatcact gaaataaaag ggaaaaacag
    11581 agccataccg ccggctttag gtattgcatg actcatcgca tgctcaatac cttcaacgtc
    11641 agacatgata gtttgagata aatctgataa attatgtttt gaaaaatagg ataatggtaa
    11701 atttgacaaa tcgtctgcaa tttgcactct taaatgcgca ctttctttat aagtactatt
    11761 atataagttt tcatactccc tatctaacaa ccaatacatg aaaagcaaag taacaaacga
    11821 tacggcaaaa taaagccaac ggggctttgt attttctaaa actaattcgt ctatcagcat
    11881 cattataatg aatgctggta ccatattaat gcagtataca aaaaaactag aaatagaagc
    11941 tttagttaaa gcttctgcgc cctgatcact taaagcaaat ttgtttttat aaaaactttt
    12001 cattgtcaag cctccaatca tttgcattct cataattagt gaccaattgt ttatacactt
    12061 gatgtttagc cattaattca ttatgatttc cacgttctac tattttccct tctgacaaga
    12121 caagaatttc atctacagat ttaatgctgg ataatcgatg agctatcata ataactgttt
    12181 tttctttcat cagatgttga aaagcctttt gtagttcata ctcattatct ggatcaatag
    12241 atgcactggc ttcatccata ataacaatct ttgcatcttt taaaattgct ctggcaatag
    12301 ctatacgttg tttttctcct cctgacagat aaactccttt actaccaatt aaggtatgct
    12361 ctctttcggg aaatttttct aaaatcgatt cgcaaccggc taatcttagc gcgtcaaata
    12421 ctttacttgc tggcgcttga ggatttgcta tagaaacgtt atcgtaaata ctctgattga
    12481 atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct tttgaatagt
    12541 gatttaaagg cttaccacca attttaatct gtcccttatc cacattgtag aaacctgaaa
    12601 ttagtttagc tatcgtactc tttccgcttc ctgattttcc tactaatgca taaattcttt
    12661 tttgttctaa aactaagttg aaattttcta aaacacactc ttctttgtat gaaaagctga
    12721 cattttcaaa tgcaatgtca caatttttaa tctgtgtctc gttcccatat tttagttggt
    12781 cttgcttcat ttctaaataa agggagtcta gtgtttcgac tgcatatctt gcttcaaaca
    12841 agtgcatact tacatacatt attctcatga aagaaacaaa aataattcca cttaaaaaaa
    12901 acaacatcaa caagtcaaca gctaatatct tgggagtggt aagagaagtt ataaaaaagg
    12961 ttataggaac tatcaaaatg caaactatgc caaaaaatag ccattgaaaa agaacaaaag
    13021 gtgttttccc actcaaagaa tacttatatg caaacttagc ataatcgtta attgctttat
    13081 tcaaggcttt aaatgatgtt actttagcac caaatatctt tataacttga attccgcgga
    13141 tatattctac cgtttcagag cttaacacat ctaacgattc ttgatatttt tgaataaaat
    13201 ttgtattctc tgtcattttc tttaacaaga atacaccaat tactaacatg gcgattagaa
    13261 ccaatccaat tttaaagtta atgaaaaagc ctaaggcaac tgataaaaca ggtgttacta
    13321 tggcttgagc actatctgga atcaagtgag caattgccat atgtgtttta gccgcgttgt
    13381 catcaattgt ctttcgaata tatcccgaag aatttaaatc aaaaaaacga aaacttgctt
    13441 ctgttaaacc gctaattccc ttttttctca aattagtctc taagcgaaac ccaaggatat
    13501 gagaaaataa gccagaaaaa atataaaata aagctctaaa gttaaccaag caacagtttg
    13561 aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact gaagaaattt
    13621 gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa ataaagtggc
    13681 taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt atactttcat
    13741 tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta atgctttccg
    13801 taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc tattttttgt
    13861 atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt gctacaacta
    13921 acactgaaat gattccccat atacctaccg catagtggat cattgcatcc attgttgcag
    13981 cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac catataggaa
    14041 tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact tgaggaattg
    14101 ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata ctaatcgaac
    14161 tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat aaaacaattg
    14221 tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca aatatgattc
    14281 cagataataa tccaaaaatc ccgggggata tgctgtgacc aaacgcccca ccaatactac
    14341 taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa ccaata
//
LOCUS       DANJJH010000072        14378 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000072 DANJJH010000000
VERSION     DANJJH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(182..544)
                     /locus_tag="PWS53_002049"
     CDS             complement(182..544)
                     /locus_tag="PWS53_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872258.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(618..998)
                     /locus_tag="PWS53_002050"
     CDS             complement(618..998)
                     /locus_tag="PWS53_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL0872259.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1062..2255
                     /locus_tag="PWS53_002051"
     CDS             1062..2255
                     /locus_tag="PWS53_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0872260.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2332..2916)
                     /locus_tag="PWS53_002052"
     CDS             complement(2332..2916)
                     /locus_tag="PWS53_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0872261.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3117..4115
                     /locus_tag="PWS53_002053"
     CDS             3117..4115
                     /locus_tag="PWS53_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0872262.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4227..4736
                     /locus_tag="PWS53_002054"
     CDS             4227..4736
                     /locus_tag="PWS53_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL0872263.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4766..5425)
                     /locus_tag="PWS53_002055"
     CDS             complement(4766..5425)
                     /locus_tag="PWS53_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL0872264.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5427..6191)
                     /locus_tag="PWS53_002056"
     CDS             complement(5427..6191)
                     /locus_tag="PWS53_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL0872265.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6199..6876)
                     /locus_tag="PWS53_002057"
     CDS             complement(6199..6876)
                     /locus_tag="PWS53_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL0872266.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6913..8298)
                     /locus_tag="PWS53_002058"
     CDS             complement(6913..8298)
                     /locus_tag="PWS53_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0872267.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8508..8592)
                     /gene="ffs"
                     /locus_tag="PWS53_002059"
     ncRNA           complement(8508..8592)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWS53_002059"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8615..9130)
                     /gene="tadA"
                     /locus_tag="PWS53_002060"
     CDS             complement(8615..9130)
                     /gene="tadA"
                     /locus_tag="PWS53_002060"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL0872268.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9237..10094
                     /locus_tag="PWS53_002061"
     CDS             9237..10094
                     /locus_tag="PWS53_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872269.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10175..10510)
                     /locus_tag="PWS53_002062"
     CDS             complement(10175..10510)
                     /locus_tag="PWS53_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872270.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10577..11947)
                     /locus_tag="PWS53_002063"
     CDS             complement(10577..11947)
                     /locus_tag="PWS53_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL0872271.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12025..12561)
                     /locus_tag="PWS53_002064"
     CDS             complement(12025..12561)
                     /locus_tag="PWS53_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0872272.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12870..14351
                     /locus_tag="PWS53_002065"
     CDS             12870..14351
                     /locus_tag="PWS53_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872273.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4427 a         2868 c         2343 g         4740 t
ORIGIN      
        1 tgtgaaatga tttttcgatc aattattatt attcgttata caaccgtttt caacatattt
       61 tgaaaataaa atgcactaaa aaactatctt tagcagaaca tttcattgta ttttttggac
      121 caaacaataa aaaaagaagt ttgaaaaaaa ttcaaacttc tttgttcgct taatctgtat
      181 tttagaagaa aggcactccc tctacttcgt aagttccaag aacagagcgc atttgacctg
      241 gttcttccca aatatatcct tcgcctaatt cttctctttg attcttttgt tcagtttcaa
      301 agacaaacaa cggaacattt tcctctttaa tatgtgtatt tgtcaattcc atcaaactaa
      361 tcttgctaac atcgatatga actatttctt ttaagaaatt aagtatattc gctaaaccag
      421 tctttccttc agaaagttcc gctaacgcaa attccaactt gtcatcaatt gaatggacca
      481 aaaatttttt agtaccatcc tccaaatgca acataattgt accagcaact cttttgcttg
      541 ccaaaatttc cacctcgatc aacttgttct atggcttaca taaaaattct agcacatttt
      601 cagaaaaaat acacttttta tttcttattt tctttccgtt ctagttcaga taatgcttct
      661 tctatccaaa taggtaactg ctccgcaata gtggtaaatc cgatcttacg atttttattc
      721 gtgaaatacc tttttctgcg atatggaaca gctggatttg ttttttctaa cgttcttaaa
      781 gataaactga tttttttgga atattcatca atgtcgatga tctgaactgt tactggctgt
      841 ccgactttta acaatgaatg gatatttttc gtataaccgg attgtacttc tgaaacatga
      901 ataagtcctt gcgtctcgtt atcaagagaa acgaatgcgc cataaggctg tatccctgtc
      961 acttttcctt caattatttg tccaatttta taagtcatct acttccctct cttcgcattt
     1021 atcattatac tgtaaatagc tcaaaaaaag aagcgacgaa aatgactgga ggtcttaaga
     1081 tgatcgaatt tgataaacag atttcaagaa aagatacaga aagtgtaaaa tgggacgcaa
     1141 ttgctgagac atatcaaatg gatgacttac ttcctttgtg ggtagcagat atggacttct
     1201 tgtctcccgg tggggtagct aaagcgttta gtgaatacat caaacatgga atctttggtt
     1261 atgccactct gtctgataaa ctatatcagg ctatcattca ttgggaacgc caacaccatg
     1321 ctgtggagtt gacgaaagaa aatatcgttt ttacaagtgg tgtgttaacc agtttagctg
     1381 cagcagtaca aacttttaca aaaccaggcg attctatttt gattcatgac ccagtttatc
     1441 ctccattttc ctctattata gaaacaaatc aacgtcacat cgttcgaagc tctttgctag
     1501 aaaaaaataa tcatttcatc atggacttgt ctgacatgga gaaaaaaatc gaagaaaaca
     1561 atgtaaaagc aatgatttta tgcaatcctc acaatcctgg agggcgtgtt tggagcaaag
     1621 aagaattgaa acagttaagc gaattgtgtt taaaacatca agtgattctg tttagtgacg
     1681 agatccatca agatttaact ttattcgatc atacatttac ttccatgcta acgattgatc
     1741 ctaatttaga tgatctacta gttgctttta cttccgcaac caagactttc aatctagctg
     1801 caatcaaaaa ttcaatggta tttatcaaaa atccagagat gaaagctgat tttgaaaaac
     1861 atttagtgat gaaccaacag cacgaaatca acacttttgg actgattgga acccaagcgg
     1921 cttatgagac gggtggagaa tggttaagcc aactgattcc ttatctagaa aaaaatgttc
     1981 aaacagttaa tcagtttttt gaacagtatc ttccgaaagt gagaataatg aaccctgaag
     2041 gaacttactt aatgtggtta gattttaccg cctatatttc agacgaccaa gaattggaga
     2101 acactcttgt tcggaaagga aaagtcgtat taaatcctgg catcacgttt ggtccttccg
     2161 gtcatgggca tatgcgcttg aatattgctt gtccagaaga aactttactg gaaggactta
     2221 atcgaatcaa gtatgcttta gagacaatcg attaaatcat ttattcaaaa taaaaggacg
     2281 agaccttact aggtttcgtc ctttttcata attactccgt accataccaa actattctga
     2341 aatttcaatt gtttcaatca ctacatcatg tacaggtcgg tcttgtggac cacgttgtac
     2401 atttgcgatt tcatctactg tgtccatccc ttctagtaca tgtccaaaga ctgtatgtcg
     2461 gaaatctagc caaggagttc cgccaccttt atacgcttca atgatttctg atgggaatcc
     2521 agcaccttct agttgtccaa gcatattagc tggcacattt gtgttattta caataaagaa
     2581 ttggcttccg tttgtgtttg gacctgaatt tgccatagat aaagctccgc gaaggttgaa
     2641 cagttcacga ctgaattcat cttcaaaact ttcgccataa atactttcgc cacccattcc
     2701 tgttccagtt ggatcgccgc cttggatcat gaaatcagga ataacgcggt gaaaaatcac
     2761 gccatcataa taaccttttt tggaaagttc aatgaaattt tgaaccgtct tcggtgctag
     2821 ttcagggaat aattgaacag taatgtcacc acggtttgtt ttgataacag cttttggtcc
     2881 tttttcactt tttaaatcta gttgtgggaa tgccatattg tttcctccta atatttgcat
     2941 acttcataat aataataaca taatacctaa cctctcgtaa aaatactaga tttgttttct
     3001 tttatttttc atcactagat acattctata acttaccgtt tcgatttcga attcaaacat
     3061 ttttgagaag cattctcaat taaattctgc tatagtgtga aaagggagga atttttatgg
     3121 aaatctatga cattacaatc atcggtgcag gtcctgtagg gatgtttgcc gctttttacg
     3181 caggaatgcg ccaagctaaa acaaaaatca ttgacagtct acctcaactt ggtggtcaat
     3241 tagcaacttt gtatcccgaa aaatatattt acgatattcc tgggtatcca gcaataaaag
     3301 caagtgaatt aatcgatcaa ttagaaaaac aattaactac ttttaaccat acattccatt
     3361 taaaagaaga agttctctcg cttactcgtg aagacgaagt tatcgagatt actacaaata
     3421 aaggcatcca ttattccaaa gcagtcattt tagctttagg aaatggttca ttccagccaa
     3481 gaaaattgaa tttagataat gctgaatcat ttgaaaacca cggattggat tatttcgtga
     3541 atgatttgat gaagtatgct ggaaaaaaag tcgctattgc tggaggtggt gattctgcta
     3601 tcgattggac gttgatgtta gagcctattg caagcgaagt ttacttgatt catcgtcgcc
     3661 cggaatttcg tgcgcacgaa catagtgtat cacgcttgaa atcttcatct gtcaacttgc
     3721 ttactcccta tctcatcgat ggattatcag gcaataatgg ggaactgaca gatatccggt
     3781 taaaaaaagt gaaaagtgac gagacgattg atttgatgat tgattctctg atcgtcaatt
     3841 atggattctc ttctaattta gaacatcttt cttcttgggg acttgatagt accagaaatg
     3901 ccattactgt aaaatcagat atgtctactt ctattccagg cgtttatgca gctggtgaca
     3961 tctgtaccta tgaaggaaaa gtgaaactga ttgctactgg gctcggtgaa gcacctactg
     4021 cagtgaacaa cgctttacac tatattaatc caaaggaacg aactcagcct ggtcacagca
     4081 caagcttata cgataaaaat cttcgtccta gttgattttt tatatctctt tgcatttcaa
     4141 gttaaacccg aaagcagttt cttgcatttt tttcatgttc cgttaacatg aaatagagaa
     4201 agaaaaaata aagggggagc aataatatgg tcgtcaaaac tgagacttta gaagaaaagg
     4261 caagagaact attgaaggaa cgtggcgtaa ctgtagaaga tatcgcacaa cttgtcatgt
     4321 ttttacaaaa agactatatt tctgatttga ctttggcgga ttgtactgaa agtgtcaaca
     4381 gcgtgctaac aaagcgtgaa gtacataacg ccattatcac tggaatccaa ttagatatat
     4441 tagcagaaaa gaaaaaattg atgagtccgt tgcaacatat tatcgaagat gacgaaggcc
     4501 tttacggtat cgatgagatc ctagccttgt caatcgtgaa tgtttacggt acgatcggct
     4561 ttacgaatta tggttatatc gacaaggtga aacctggtat tttaaaagaa ctaaacagcc
     4621 acgatggcgt tcatgtccat accttcttag atgatatcgt cggagcgatc gccgctgctg
     4681 ctgccagtcg attagcacac aaagaaccag aaaaaagaag tcgccttact caataattca
     4741 acaaaaaaag aaagaaaacg tctatttaga cgagttcttt cttttttgtt tgtttcaagc
     4801 tattttttcc taagaaaaga aaaacagcaa aaaataattc taataataca aaaaataaga
     4861 taaagctatg catgaaaaat tcttctggaa gtacattgat gattggatca gtaacgggat
     4921 cgaataacca gtcatcgttg ttgaaaaacg tttcatgaaa aaggataaaa aagcgatcaa
     4981 atcctatcat catgaaaaaa ccgaaaacaa ctggcagtaa catgccgatt tgaaacggac
     5041 gaatcaaccg ccaaagccga tcattctttt gcaaatattt gataaacaaa atactcggga
     5101 tcaaagtgat gaaaaacaca gcataatcca ctaaaaacaa aattttcact tcataaaagt
     5161 gatgggctcc gctagcagaa actgggaaat ctggcaagct gaggatggtt ttgaagggat
     5221 tattgagata attcattagg tgatcaaaat ttttcagtaa cgtctcttga tctaaagaag
     5281 tataatccaa aatttgcaaa tgtccgatat caaatacgta tagtggtcga aagttgatcg
     5341 tcacaccaac tgccagactg atcagtgtca gaaataggct gaacaatcca gcatattcca
     5401 tccactgcca tctcgaattt ttcatattag aagctccatt catccagtga ttgtttcaca
     5461 taggtagctg ggataggcaa cccaggtaca tcttcttctt ttgtaaaacc tgataaaacg
     5521 agcaaagtat ctatattatt gcgtatccca gcttggatat ctgtttcata gttatctcca
     5581 accatgatta cttcttcttt ttgcagccct aatacttcaa cggctttatc cataatgatg
     5641 gcttctggtt ttccgatgta gatcggcggc gtttgggttg ctgtctctac aaaagagatg
     5701 actgaccctg ctccaggaag aaggccgcgt tcagtaggta tattcttatc tgggttcgtt
     5761 ccaatgaaag tagcaccttt ttgaatagca agtgttgctt taactacttt ttcataggtc
     5821 agataattat ctaagcctac cactacatag tcaggagcct cttcttccca tgtaaatccg
     5881 gcagctaaaa tcaagtcaat caatcctgct tctccgataa catagacttt tttccctttt
     5941 ccatctgctt tcataaaatc gattgttgcc aaagtagctg tgtagactgt ctctggagct
     6001 acatgaatgt caaactcatc tgctagacga ttagctactg tttctggtga cttagtcgtg
     6061 ttattggtaa caaacaagaa aggtaatttt cttttttgca attcttccac aaatcgttta
     6121 ccggccggta tgggttgtgt cccacgataa atcgttccgt ctaaatcaat taaatatcct
     6181 ttatagtcca ttcatccact actcttccct ttttctgatc gtaaatgctg tttttgtttt
     6241 ttctacttgt ttaggttgtt cttgtttttt tactttttca gaggtttttg ctgactctgt
     6301 ttttcgattt ttaataaccg gttttttgcg attagatgcg actggtcctt ttttctcatc
     6361 gatatacgca cgattatggt tttttttctt ttttcggcgc tgagttgtct tttctttttt
     6421 accgccgatt ctctcgatga caaaatacgc acaaccaaaa ttgcaatatt cgtaaagata
     6481 gtcttccaag gaatcgattc gctgatctgg atgcgcccgt cgattatccg cttggaaaaa
     6541 tccttttagg cgtagttgtt cataacccca atctcctacg atataatcat atcgggctag
     6601 tacatcactg taacgttctc caagcttttc tgcattaaat ccttcacgat aatcggacac
     6661 aagccgataa tgtctttcac ctatcaaaaa attactgtca tctaacagtg tcactagctc
     6721 gcctttgact ttttctgctt taggctcctc aacgatttct tccaaagcag ctgtcagttc
     6781 ttcggtgact gttgtttcct gttcagctga tgattttttc ttagcaattt ctgttttatg
     6841 ctcaacgttc tgctgttcat tttgttttgc agacatgcgt tcaccaccta tcttttttat
     6901 tataccttgt ttttatttga ttgggtagtg agctttcaag taatctccta ccacacttcg
     6961 gataaattca gggaacaaaa attcattttt tccagcaatc tcaatcgttg gaaaaaatgg
     7021 aacaaacatc aaatgatcta cggtagtaaa agtataattt ttattcagat cgattggctt
     7081 gttcttccaa aagacttgat ggttaataga atcatatgtg attccattat agaccatctg
     7141 cccaaaaatc ttaccgcgaa atcccattcc cttcatagga tagttacgta aaaagttacg
     7201 atttttttct atttctaaaa ctaagcggat cagatcattt ccacccaatg tgacatttaa
     7261 caagtgcatc ggatgcggca acgcagtgtg aagctgatcc tgattgaccc gcccttttgg
     7321 aatttcagtt aaaaacagac cactgtttaa tattgctgct tccgtctttc ctcgttcttt
     7381 caccgcctca aaggctactt ggataaacga atgctcattg aagatatcta gactcagaga
     7441 aaatggcaaa tctgctactt ctttttcttt taataattca tgcccacgtt ctagataact
     7501 atttatttct tgttcatctt ctgtaaaagc tgtcattgtt tcggtaggga tggctttggc
     7561 tgaatgctta aggatttttt tctcttcatc gatggttaaa tggacttcac cgacatactg
     7621 cccgtacttt ccagcggctg ctatttggac atcattgatt acgtgacctt ctctgaataa
     7681 atgatgggtg tgcgatccta aaataacatc aattgctggt atttcttttg caatttggaa
     7741 atcatcctga atcccgagat gactcatcaa aactaaaaca tccacttgtg aacgtaaatc
     7801 ttcgactaat tctggcaaaa tatcataagg attccgtatg tcccagccat tcggattata
     7861 agtaagcgga aatggagcag taaatgcaat tagtccgatt ttcgtctggt gtttcgttgt
     7921 aatgatttta tatggtttcg tccatgccgg tggctggagc gtatttttat cgaataagtt
     7981 atccaggatg atatcaaact cagcatgatc gtataaatga ttcaactctt ctttggaatt
     8041 tcctacgcct tcattgtttc ctatcgtcgc tgcatcataa ccaacttcat tcatcagcaa
     8101 gatattttcc tgtccattcg ttgcttctga caatggatgc caacgatcca caaaatcacc
     8161 tagatctact gcaatcgttg tcgttttttc tttcttttcg ttttctcgtt tacgttgatc
     8221 caaaaacctt ctgatccttg gccaattttc taaatgagaa tgcagatcat tcgtatgtaa
     8281 aatcactatt ttttccatgt accctctcgc ctctctttat ctttatcata ccataggcag
     8341 ctttttcttc cattgaaaca agctctattt cagagttttt cctgccttga agcaaattgt
     8401 ataagtacaa tattcgataa atcagtctgg ctaaaaaatc aggaacattc atttttacag
     8461 tactataaaa ataaaaaaaa cagacccgaa agtctgttgt cgtttcatta taatcagaca
     8521 catggcggaa cctgcttagt gctgcttcct tccagacctg acacgcttca caagcccacc
     8581 attgtcctag cctttcggca aagaatagta taccttattt ttcattcgtt gaccagtctt
     8641 tttttaattt cttttttcgc tggcggagct ttcggaaaaa gtcagtcaaa atcatcccac
     8701 attcgtcttc taaaatgcca ccttctacat atgcttggtg gttaaaccgc tcgtcctcca
     8761 aaagattcat caaggtaccg gctgttccgc cttttggatc atacgcgccg aaataaactt
     8821 ctgctacacg agacaaaatc atcgcgccgc tgcacatagg acaaggctct aaggtaacaa
     8881 aaagtgcagc atcttccaag cgccagcttc ccactttttc acaggcttcc ttgattgcaa
     8941 gcatttctgc atgtgtagta gcatcttgcg tggtttctct aaggttatat cctcttccaa
     9001 tcacttttcc atccaataca accactgctc caatcggaac ttcatgaagc atttctgctt
     9061 tctttgcttc tgccaatgca aggcgcatcc atttttcttt ttctactgca tcaagatttg
     9121 tatttaccaa ttacccttcc ctgcctttgt tttttcttgt tttactttag catatgataa
     9181 aaaggctgta actaactttt ttttctatgg taaaataaat acaagaggtg aacaagatga
     9241 aaatggataa attacttgct ctttacccac aagcacagaa aaaaaactct ccagcatcag
     9301 aagcggatat cctttctttt gcaattgatg atgcttttat ctggatcaaa caagattcat
     9361 tatctcaaca agaaaccagc cttttgaaag cgttattccc tgtaatcaac gatcaaaaaa
     9421 aacacccatg gtatcgctat ctttttaaag atgcttcttg tgttcaagaa aaaagctttc
     9481 gtttgatcca gcttcacgtc gaatcaaaag gagaattctt aaaaaaagaa tggcaagata
     9541 ctattttgga attatttcgc tattctgctg atttcttttt ttatacagac acagatgcgc
     9601 tcttagtcga acaaataaac cgtgattatc ttgatgctac tgaaatcaac ggtatttttc
     9661 tatctttaga tgctgatttt gatacgacaa cgtctgtatt cgttggttct ttccattcac
     9721 ctggcaccga cctcgtccct ttgtttgctg aagaaagaaa aattttttta acagagcaaa
     9781 acaatcttta tactcacagc cgtacttttt ctatgcaaga tgtggcttta cattactata
     9841 caaaagatac tgtaaaagat agtaagttga tcaaaagtta catagaactg atcaaacaaa
     9901 atgaaatgga agtgatcatt caagaattat ggaatgaatt aggcaatgtc agctctgctg
     9961 caaaaacact gtttcttcat cgtaatactc taaaatatcg aatcgaaaaa tttcaagaac
    10021 agtctggttt caatctaaaa aaagccaacg atttactgtt ttgctatctt ttattgcttc
    10081 atgaacataa ttaaagatag attaaagggg ttgtgacaat aattttgtca caacccctta
    10141 tttcgaatga atagtgttca aaagctagtt tctcttacat cattttttca acaaaatgca
    10201 ttagccggtc ttttacatct tcggttgaat ctttgattga atcaccataa acagagattt
    10261 tcttttttcc tgatttgatt tttgctaaca ttcccatcaa agaatccaag tcactatctg
    10321 aaagaccatc tacgatttct agtaattttt catttccatc aaatttgtca tagacgaatt
    10381 tttttacttt cgtccgagta gtcatatgat ggatcttttc cattactttt cccgatacaa
    10441 cgactgctgt cgctacactg gcagcggctg ctacgcttaa tccgattgtt acttttgttg
    10501 atgttttcat aagtatcagc tcctttttat ctatctccat catagcatag acaaaaaaat
    10561 aaacgaacta atccactcag tgaaatctca atcctttagg gaaaaagttc tttactgttc
    10621 cttttacttg tttcccaaat ccgacaggta tcttatctaa tactagcaaa acccatcctt
    10681 gattcccatc acgttgaaat gtttctcctg ctgtgtaaga ttgccattcc ttttgagtga
    10741 tcggcaagca aggtatattc tctatctttt ttgtagcaag cgctaatgca tagctaggtt
    10801 caaatcgatt ctttttgaaa tctcctaaat gcaaacctgt acgtacgact tttaaaccat
    10861 cgagagaagg ggctaactca ggaacttccc aaagatggtc gttgaacact aaaagtcttc
    10921 cagtagcttc ataatgaaaa tcatttgaaa actccgtcca gagtttttcc tgctcttttg
    10981 tcatttgcac tttacttttt tttcgtgtct tcttttcttt atgcatctga ttttgtccat
    11041 gaaaagtcag ctttgcaaca aaatgtcctt caccctgatc tttatgaggc catatacgta
    11101 ttgttttttc caaaccagct acgcttcccc attcagaacg tcctgaacta acggattgag
    11161 tcaaaggaat ctcctcaatc gttactggat aattttctac aagccagcta atgatctctt
    11221 cattttcttc tggtgcaaac gtacaagtcg aataaatcag ttgtccttta ttttttagca
    11281 tttttattgc tgaagacaaa atctcttgct gacgtttttg acagtatagc ggagattctt
    11341 ctgtccactc ttttattgca tttggatctt ttcggaacat tccttctcct gaacaaggag
    11401 catcaaccac gatccgatca aaaaagcccg aaaaatgagg gacaagttct gcgggtgcat
    11461 gatttgtcac tattgcgttc gaaactcccc atcgttctat attttctgat aaaatcttcg
    11521 cacgtttcgg aaagatctca ttcgtcacta ataacccttt ccccttcatt tgagcagcta
    11581 attgggttga tttaccaccg ggagccgcac acaaatccaa aactttttca ccgggttttg
    11641 ctgcagcagc tgttccaacg atcattgcac ttggttcttg gctatattca tacccagctt
    11701 gatggaggaa agactttcca ttgacagtgc caagaaatcc ctcattgctg tagggagcgg
    11761 gttgtaattc ttcgctatgg taagtttgga ccatgtccaa tcctgccggt tttagtggat
    11821 tccaccgaaa tccttttttc acgcttcctt gttcaagcgc agaaaaaaaa tcggatgctt
    11881 cttcgcctaa aagaagacga tattttttga taaattgctg tggtaacgtt gtcgcttcct
    11941 ccttcattcc aatgtttcgt tagacatttt acaatctttt ttacgaaaaa taaaggtggc
    12001 tgatctactt tttcatgttg ctgcctaagg tgactgttcc tttaagatat tttccaagta
    12061 ttcatccgtc acctcgtttt tatcccaaac agcgttcatc atttcaagaa aatcttgaga
    12121 agaatcatct gagcgcaaac ttcccaatgc attaagccat accaattcaa gcgacttttc
    12181 ttctttgatc ttcgtgtgac ctttgacata tgagaaaaat ggccgcttaa atagcgaatc
    12241 catgtctaaa tcagattttg caatgatttc acgaatactt tcatggatag aaaaaaaatc
    12301 tttcaataag tagacaatgc ttaaaacatc atcggaactg cattcagcta agtagttttc
    12361 aaaataaaca gtgatttctt ctaatatctt ctcttttaac ccgctgattg atttgaaatg
    12421 tgtataaatc gggtatgtag aacaaccgca gaactctgct aactctcttg cagtaattga
    12481 cttcattcca tattcttcca cgtataccat acttttgaac agcaaatatt ctttactata
    12541 atttgttact ctccccatca ttcttacctc cgtctctttt catctaccca taaataaata
    12601 acataaataa ttctaaaaat attattgata attatcaaaa catatttagt tatatccatt
    12661 aaaaatcaat ttttccgcct taaaaacaag aatatcttga caaaataccc taggaaataa
    12721 acaatagaat aaacaaggta acaaaaaaat taaataacgt tatataaaaa agaaggcgac
    12781 actctgtgag aataacacag actgaaatga ctttccactc acagtttttg tctaactata
    12841 tacaaaaaca gaataaaaag gaggtattta tgctaattga agcaagaaaa aaagagttac
    12901 ttgttctaca aatggtcatt caaaaaaata attgttcttt acaagaatta tccaatgact
    12961 tacatattcc aaaacggaca atcaaagaac tgatccgaaa aataaatgta tcactcaagc
    13021 aatttttatt gattgatgaa tttatttatt ctaatcataa aggagagatc caaaccaaag
    13081 aaaccgatca aactaaaaag ttagctgttt tttctaagtt aaaattatgc tacctaaaag
    13141 aatccaatcg ttttaactat ttgatgttac taatcaatta tccacaaact gctgtaccaa
    13201 aaaaatattt acttgaacaa ctttatatat cgccctccta tctcgaaaaa ctgacaaaac
    13261 agctcaatca ttccttaaaa aaattccatt tacggatcct atctgtaaaa aattgctgta
    13321 ctcttgaagg aaatgagtta tctatccgtc tctatcttta cttttttctt tcagatacat
    13381 ttggaggtaa cgattggcca ttttcctcac ttgaattaaa tgcactaaaa atgaacagta
    13441 cagatgagca aatagaaaaa atttcaaaac aagaaaaaaa tatctacttg ctgttaactt
    13501 tattcatatt acgaaacaaa caacaggcat attttccaaa aacttcttct gaaatcactg
    13561 aattgatgga cttattgcag catacacatg attattcaga atactttact tctttttcag
    13621 tgagtcgccc ttgtgaagag acagaaacag agagagccta ctttaattat tttttctatt
    13681 gtttttttcc aaatcaaatc tcttttcgac aaaaacagga gattggtttt ttattttcca
    13741 caagcaaaaa ccttatttgt cgacaagtcg gtcaattgat gacttactta actcaacagt
    13801 ttcctctcaa ccatttagaa aataaaatac caatttattt ttattttctt gtgttggcag
    13861 ttgttttttt cgaactgttg ggtgaatcca gcagtagttt ttctacactt tgctttccag
    13921 aagccacgtc caatcattct agttctttta tacaggaaaa gaaattaaga gaaaaaatcg
    13981 atacaatttt ttcaaaaaat ggggaaagaa accaaatcag tcaatacctt agtcaattga
    14041 tccaacactt actacaaaca gaaacgaaag agcagttact catccatttg cagataacca
    14101 aacagtttac aggaaattat catcttcaaa gccatttgca gcagttattt cgcaaagatg
    14161 ctgttaaaat taccgcatat tcagaacatg ctgatttgat cattacagat cagcatgaga
    14221 aaatttcaga tgaaacggag actttctatc tggatacaac gcacaataca gtgttatttg
    14281 gcaagttaat aaaagcggtt catgaactgt atatgaaaaa aggttatgag aacaaaacaa
    14341 agaggatgtg acaaaattct tgtcacatcc tcttattc
//
LOCUS       DANJJH010000073        14101 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000073 DANJJH010000000
VERSION     DANJJH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            1..248
                     /locus_tag="PWS53_002066"
                     /pseudo
     CDS             1..248
                     /locus_tag="PWS53_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            379..1329
                     /locus_tag="PWS53_002067"
     CDS             379..1329
                     /locus_tag="PWS53_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0872274.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1847..2302
                     /locus_tag="PWS53_002068"
     CDS             1847..2302
                     /locus_tag="PWS53_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0872275.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2643..3245
                     /locus_tag="PWS53_002069"
     CDS             2643..3245
                     /locus_tag="PWS53_002069"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872276.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3339..3671
                     /locus_tag="PWS53_002070"
     CDS             3339..3671
                     /locus_tag="PWS53_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HDL0872277.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3822..4801)
                     /locus_tag="PWS53_002071"
                     /pseudo
     CDS             complement(3822..4801)
                     /locus_tag="PWS53_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4806..5573)
                     /locus_tag="PWS53_002072"
     CDS             complement(4806..5573)
                     /locus_tag="PWS53_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0872278.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6063..6488
                     /gene="lacA"
                     /locus_tag="PWS53_002073"
     CDS             6063..6488
                     /gene="lacA"
                     /locus_tag="PWS53_002073"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL0872279.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6505..7020
                     /gene="lacB"
                     /locus_tag="PWS53_002074"
     CDS             6505..7020
                     /gene="lacB"
                     /locus_tag="PWS53_002074"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL0872280.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7031..7963
                     /gene="lacC"
                     /locus_tag="PWS53_002075"
     CDS             7031..7963
                     /gene="lacC"
                     /locus_tag="PWS53_002075"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL0872281.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7966..8946
                     /gene="lacD"
                     /locus_tag="PWS53_002076"
     CDS             7966..8946
                     /gene="lacD"
                     /locus_tag="PWS53_002076"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0872282.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8974..9291
                     /locus_tag="PWS53_002077"
     CDS             8974..9291
                     /locus_tag="PWS53_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0872283.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9291..10997
                     /locus_tag="PWS53_002078"
     CDS             9291..10997
                     /locus_tag="PWS53_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0872284.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11139..12545
                     /gene="lacG"
                     /locus_tag="PWS53_002079"
     CDS             11139..12545
                     /gene="lacG"
                     /locus_tag="PWS53_002079"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0872285.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12568..12840
                     /locus_tag="PWS53_002080"
     CDS             12568..12840
                     /locus_tag="PWS53_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDL0872286.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12861..13760
                     /locus_tag="PWS53_002081"
     CDS             12861..13760
                     /locus_tag="PWS53_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL0872287.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4603 a         2343 c         2732 g         4423 t
ORIGIN      
        1 atctaatttt agtgataact taattgaagt tcttacagaa ccagaaattc ggtctatgac
       61 ggatgaagaa caacagatta ttaaagctat gatttcggaa aataagaaag acgtagtagt
      121 tatcaaaaag attagagagt atacaaattt aggtgtttta caagccaaaa aagtatatga
      181 aaaaaaaaaa aagggtctat aattacaaat tagataatac attagaagct ggagacaaga
      241 ttgtctagat aatgtgtgag ataaaatttt tggcaggtta tcatacataa gacaaaatgt
      301 ttttaaaact gataacgtat ttattccaca acaattacgc atttatttct gagaaaaata
      361 taaagcagga ggattacaat gctgaaaact tattccttaa caaaagatac attattagtt
      421 tcgtcagaat caaatatcaa aaattcttct gttgtttatg tttatggtga cgaacgagaa
      481 tatatcgaat cttctgaaca taaatatgac tttaaaatag gcaatatact aacgtttgat
      541 gatcgagttg catatgacac gatgatagat aagaacgatg aaaaaagttt gctcgtaagt
      601 tttttgtttc ccgaggtaca tgaaggtggg catctagata ccctgacgac atctgttgtt
      661 ttcttggtat ttaaaaataa gttgataatt tttacaaaac aaaaattaaa attcattccc
      721 gaactcttat ccaatatggt tttacgtgat gtcaatcatt tcgtggagga agtactttta
      781 aagatcatgc aaaaagattt ctatatgatg cttaacgacc ttcgtggagt caaggaaaaa
      841 attgatgatt tagactcaga aatatcaacg tccggttcac tcaaaccaac gtttggtgat
      901 ttattgactc ttcaaaaata catgatagct ttgtcaacaa cttataaagc taaccataaa
      961 gcgcttgatt tcattaaaaa aaatttcaca actattgacg atattgattt tagtactaaa
     1021 aaaataattg atgatttata cgatacaagt gatacaatgg atagaatcat tttaagttac
     1081 agccaatatt tggacaaatt ggaaaatatg ataaacaata tgatttcgta tcagctaaac
     1141 atgattatga aaaccctaac cgagatatca attgttttga ctattccagc aattattttt
     1201 ggcttttggg gaatcaatgt ccatgtcccg tttgaaaagt cttcttatgg tgttttagcc
     1261 gtattgatta tctctgctat actaagtcta atttgctggc ttttgatgcg acgcaaaaca
     1321 tatttgtaaa gtatttgtga taccctaata tgatttaaag cagaatttct ttgttgactt
     1381 tttaaaaggc tgattccttt caataattta atagcttcag cggctgctca gaagtcaata
     1441 aattggacta tttttattca gttacttagg caacatttga gatacgattg cgattataaa
     1501 aattgatata ccaaccaatg gctgcttgga agactgacat attagcctcg atatcatcga
     1561 ataactaaat acagaaaaat acaataaata ttatcaaact agggactata ctagaaaaca
     1621 tcagtacatt ccttaactca gggtctataa ttagaaaatc gcccctttga aaactgatta
     1681 aaacagcccc cattatctag tgactagata atgggggctg tttttttgtt aagtgatata
     1741 ataaaccata aaagaagttt agaaatacgg tcctggtcat caataaataa acaatttaat
     1801 taattattta tttaattatt taatttcaga aaggtataca tgaatcatgg tgcaacaaat
     1861 tgtcctaccc atcaaagact ccagcgtctt aaagatggta caagatacac ttctcgatag
     1921 tttccgtgct ggtcgccgaa attacaccgt ttttcaagtt ggtaaggcca cgctactacg
     1981 tgtgagtgat gtcatgacat taaaaaaatc agatgtctat aatccagacg gctctgtcaa
     2041 aaatgcaacc tttattcatg ataaaaagac cggtaaagca aacacgttat atttaaagcc
     2101 tgttcagcaa gacttgttgc aatatcatga ttggctggcc caagaaaata tcaattctga
     2161 gtggttattt ccctcgacag cccattatga tcttcatatc actgagaagc agtttcataa
     2221 ggtgatggcg catgttggtg atctcttagg aatcaacttg gaagacaaag agaaaaaatt
     2281 gcgagtcaga aaaccgtttt gagatatgaa cagcatttgc cttcacgcct tagcgatttt
     2341 aaataatagc cttgaaagtg attatggaaa agccctctac gctcaatgca aaattgacgt
     2401 agaaactatt tttgatcgat tgaaaggtgt atttggtatg tgaagaaccc atgtcagagg
     2461 caagaacgtt gcacctactg acattggaat gatgcttatg agcatgaatc taaccaaatt
     2521 atcccttgag gctagaagaa aagcggaagc ttttcaacac aaagcagcca aaaataaaaa
     2581 acgcgacgaa gcaatcacat ttatgattat tcatcgcgtt ttttatattt agagctagtt
     2641 atttgctaga cccactattt acagatgaaa cgtttatcac ctttggtttt gggcatttag
     2701 tggaattagc agaaccaggt cattatgacg aaaagtggca aaattggaaa cttgaatctt
     2761 tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga aagcagttta
     2821 aaattgttgc agaacttctc aaaaaggcaa atacaattat tgttgcaaca gatagcgaca
     2881 gagaaggtga aaatatcgcc tggtcgatta tccataatgc aaatgccttt tcaaaagata
     2941 aaacatttaa aagactatgg atcaatagct tagaaaaaga tgtgatccga agcggttttc
     3001 aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa acacgccaaa
     3061 ttgccgattg gttgatcggc atgaacgcaa gccctttgta tacgttaaat ttacaacaga
     3121 agggcgtaca aggtacattt tcactaggac gtgttcaaac gcccacctta taccttattt
     3181 ttcagcgcca ggaagccata gagaatttta aaaaagaacc ttttttcgag gtggaagcta
     3241 agtaaaacaa gggtcattta agggcgttct aagccccaca cagcgtttta aaacccaaga
     3301 ggagcttttt agcttttgtt tcttctaaac aagctaaaat aggcaatcaa gaggggataa
     3361 ttgctgatgt tcaaaccaaa gagaagaaaa cgaatagtcc gagttcgttt tctttaagta
     3421 gtttgcaatc aaaagtcaat cagctttata aagcgacagc gagccaaact ttaaaagcta
     3481 tgcaaggact gtatgaagca aaattattga gttatccaag aacagataca ccatttatta
     3541 cagagaacga atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac
     3601 ttgatcttga aatgttgtta tcattgataa tgatacgttc aaacaacagc acccaaattt
     3661 tgacgaatta gtgaaactct atgaaaaaga cgtagtaaaa catgcaacat cctattctaa
     3721 tcagctggta aagcttaatt aggttatata agcttgaaaa taaagggttt gtccaatatg
     3781 gacaagcctt tttcttatgt tttgccacag atttgccaca cttattgtgt aagagtatca
     3841 aggataggta taatttgata ctttgttttt ttagtgatgt gcaaataaat ttctgttaca
     3901 cgactacctc tagaatgccc tacacggtct tgaattgctt ctaggggtat tccttactct
     3961 gctaagaaac taatatgcgt atgacggaat atatgggtcg taatatgttt attaaaacta
     4021 agtttattat caatatcaat tcctgctcgt tttggattac cttttcgatt taattttcca
     4081 ttttcaggtg agttgaggaa attgttaagg acactataat gaagaggtgt tccattatag
     4141 tcgggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
     4201 aatctcatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
     4261 gataattgga catttcggta actttgtaag ttttttacat taccttcata gctattttct
     4321 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
     4381 gcaccaatat tttggagttt cgactctgta agtcaacagg gccattagtt agaataccta
     4441 ttgggatgcg gtttttatgt agagccagca gtagttgatg tatctcagga atcatcgtaa
     4501 tctgatcgag ttcatattca tagtcagctt ggaagtctag agcttctgtc ctagaaatac
     4561 tatcgatgcc aaattcttcc atagtcttct gacagcgaaa aattctaagc tgctcaatac
     4621 tgatgagttt tttcgtgtat ttaggaaaag caacatcaga ccaatatctg aaacgtttgt
     4681 aaatgttatc tatttgttgg atatcaatcg ttggaaaaca tttctcaacg ctacgtttaa
     4741 aggggagttg atggtcataa agagtgtcat caaggtcaaa aacaatattt ttataagtca
     4801 taattctagt ccgcttttaa aatagtagtg tactggctaa attcctctaa gtgttgaggg
     4861 ctaatctggt tatctgtaat gatggtatcg agttgatcta gattatagaa gttaaagaaa
     4921 tcgtatcgat cgaatttagt actgtctact aataagaatt tttctacagc attgtttagg
     4981 gcaagttgtt gaatcacacc ttccttgtca ctatatgtag caatagaatt atgggtaaca
     5041 gcatttgcac taacaaaagc tttggcaaat ctaattgctt ttaaatttgt cgaagccatt
     5101 gaacctacaa aagctccagt tatttctcta tattcaccgc caagaagcaa taaatcaatg
     5161 gtttcgctat catttagaat caagaacaca gggagactat ttgtaatgac acgaatttta
     5221 taacctttac gacctttcaa ttctaatgcc agttgttcaa gtgtagttcc aggtccaata
     5281 aaaatagtat cgccatcttt gattaaagag caggcttttt tagcaatgtc aattttttct
     5341 tttgtattta gacttttctt ctcgatatgt gtcttttcaa gtggcttttt agaggaaagc
     5401 aattgtgcac caccatgagt acgtgttaaa agtccatcag cttctagagc atcaaggtct
     5461 ctacgggctg tcatatcgga aatatctagt tcatctatta tttcttttat ggttatggtc
     5521 ccatcaatct ttaacatatc taaaattttt tctaatcgtc gttttttgtt catatgaaga
     5581 cccttctttc ataaagtaat ttttttccaa agataattct cttttaattg tatcataaaa
     5641 gacaatattt tcaaggtaaa acaaacaatt tcaaacaaaa acaaatgtta gatgatgaaa
     5701 taagaacaga ggattgacgt atattagctt aggtcagatt ttgtataaga cgaaaataaa
     5761 gtaggacctc ttaatcagta agttatagaa agtaaaagac ttttgtaata cttgaataga
     5821 tatttcacgt ccattttgtg atggattaaa tgaacaaaaa tgaacaataa tttaacggtg
     5881 ttatctattt tttaaaaaaa caaataaaaa aaacaaaaaa taaacaaaaa aagtgttgct
     5941 ttttgtttga atgcttgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     6001 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     6061 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     6121 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     6181 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     6241 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     6301 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     6361 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     6421 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     6481 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     6541 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     6601 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     6661 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     6721 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     6781 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     6841 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     6901 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     6961 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     7021 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     7081 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     7141 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     7201 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     7261 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     7321 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     7381 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     7441 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     7501 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     7561 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     7621 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     7681 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     7741 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     7801 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     7861 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     7921 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     7981 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     8041 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     8101 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     8161 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     8221 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     8281 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     8341 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     8401 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     8461 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     8521 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     8581 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtagtttac
     8641 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     8701 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     8761 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     8821 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     8881 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     8941 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     9001 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     9061 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     9121 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     9181 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     9241 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     9301 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     9361 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     9421 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     9481 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     9541 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     9601 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     9661 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     9721 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     9781 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     9841 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     9901 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     9961 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
    10021 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
    10081 atcttatcca agctggacaa catgcagaca aagttatcac ttctggtact caaatgttta
    10141 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
    10201 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
    10261 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
    10321 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
    10381 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
    10441 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
    10501 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
    10561 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
    10621 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
    10681 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
    10741 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
    10801 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccacaggtcg
    10861 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
    10921 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
    10981 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcaatt
    11041 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
    11101 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
    11161 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
    11221 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
    11281 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
    11341 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
    11401 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
    11461 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
    11521 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
    11581 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
    11641 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
    11701 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
    11761 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
    11821 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
    11881 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
    11941 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
    12001 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
    12061 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
    12121 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
    12181 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgagt gaaaaatgat tatccaaact
    12241 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
    12301 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
    12361 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
    12421 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
    12481 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
    12541 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
    12601 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
    12661 cctgagcttg aagctacccc gttgctaaaa tgtggtaaat ggtatgtttc aactggaaaa
    12721 gagtggattt gccattctga tttagagtta tcagcatttg aatgggaatt tttacaatca
    12781 ctcgatgtag aaatacgtga gactattcac ttcgaggtaa attatttgcc tttccaataa
    12841 taaaaagaaa ggaattagtt gtgactatcg aattaaaaaa cgagtatctt actgttcagt
    12901 ttaaaacact tggtggtcaa ctgacttcga ttaaagacaa ggatgggata gagtatctct
    12961 ggcaagctga tccaaattat tggaatggac aggcgcctat tctatttcct atctgtggta
    13021 gtttacgaaa tgattgggct atttatagac ctcaagaaag accctttttt acaggtctta
    13081 ttcgtagaca tggttttgtt cgaaaagaag aatttattct ggaagaagtc aatgataata
    13141 gcgtgacctt tagtattaaa ccaaatgctg agatgcttga taattaccta tatcagtttg
    13201 aactgagagt tgtttatacc ttgaatggta aatcaattag aaccgaattt caggtaacga
    13261 acttggaaat ggaaaaaaca atgccttact ttattggagc ccatccagca ttcaatagtc
    13321 ctttagtaga aggtgaaaac tatgaggatt attatattga atttagtgag gttgagtctt
    13381 gctcgatacc aaagagtttt cctgagacag gattattaga tttgcaagat cggacacctt
    13441 ttttggaaaa tcaaaaaatt ctcgatttgg actactcact tttttcccat gatgccatta
    13501 cacttgacca gctcaagtct cgtagcgtaa cgcttcggtc aaggaagtct ggtaaaggtc
    13561 ttcgagtaga ttttgatgat tttccgaact tgattctgtg gtctacaact aataaaagtc
    13621 cattcattgc tttggaacct tggagtggtc tctccacttc acttgaagaa ggtaatttct
    13681 tagaagataa aagacaggta acgaaaattt cacctcaaga aacttctaga aaaatctatg
    13741 atattactat ccttaattaa aatttgttat tatttgagtc ttcaataaga tttgaatact
    13801 cttaccttgt gatgatagat tggtcttctc cgtgccagtc ttacatcgca aggtttttaa
    13861 gttttttgtg ggtctatgtc aactgtagtg ggtgacgaaa agctaatatc taaagacagt
    13921 ctggtagtaa aaaataagat tatttactac ttcttgattt aaaataatag tggagccatt
    13981 aatttgtggc aaatttaatt taatattttg cctcaaatta gccacacttt ttatagaact
    14041 aagaggaaaa atgaaatttt cttcctatta atatagttgt ttgaaatctt aaatatagcg
    14101 t
//
LOCUS       DANJJH010000074        14005 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000074 DANJJH010000000
VERSION     DANJJH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..352
                     /locus_tag="PWS53_002082"
     CDS             <1..352
                     /locus_tag="PWS53_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0872288.1"
                     /translation="LKPALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDA
                     IMRWYCMNVYEEHISNGNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNS
                     SVENTGTGFTPLVF"
     gene            372..1550
                     /locus_tag="PWS53_002083"
     CDS             372..1550
                     /locus_tag="PWS53_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0872289.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            1513..2199
                     /locus_tag="PWS53_002084"
     CDS             1513..2199
                     /locus_tag="PWS53_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0872290.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            2199..3359
                     /locus_tag="PWS53_002085"
     CDS             2199..3359
                     /locus_tag="PWS53_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0872291.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            3369..4244
                     /locus_tag="PWS53_002086"
     CDS             3369..4244
                     /locus_tag="PWS53_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872292.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            4241..4552
                     /locus_tag="PWS53_002087"
     CDS             4241..4552
                     /locus_tag="PWS53_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872293.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            4542..4895
                     /locus_tag="PWS53_002088"
     CDS             4542..4895
                     /locus_tag="PWS53_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0872294.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            4885..5286
                     /locus_tag="PWS53_002089"
     CDS             4885..5286
                     /locus_tag="PWS53_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872295.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            5279..5683
                     /locus_tag="PWS53_002090"
     CDS             5279..5683
                     /locus_tag="PWS53_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872296.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            5695..6303
                     /locus_tag="PWS53_002091"
     CDS             5695..6303
                     /locus_tag="PWS53_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0872297.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            6323..6685
                     /locus_tag="PWS53_002092"
     CDS             6323..6685
                     /locus_tag="PWS53_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872298.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            6688..6870
                     /locus_tag="PWS53_002093"
     CDS             6688..6870
                     /locus_tag="PWS53_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872299.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            6887..10306
                     /locus_tag="PWS53_002094"
     CDS             6887..10306
                     /locus_tag="PWS53_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0872300.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            10357..11094
                     /locus_tag="PWS53_002095"
     CDS             10357..11094
                     /locus_tag="PWS53_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0872301.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            11104..13395
                     /locus_tag="PWS53_002096"
     CDS             11104..13395
                     /locus_tag="PWS53_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0872302.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAQRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            13419..>14005
                     /locus_tag="PWS53_002097"
     CDS             13419..>14005
                     /locus_tag="PWS53_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872303.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         4936 a         2087 c         2957 g         4025 t
ORIGIN      
        1 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
       61 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
      121 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
      181 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
      241 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
      301 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
      361 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
      421 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
      481 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
      541 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
      601 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
      661 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
      721 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
      781 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
      841 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
      901 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
      961 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     1021 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     1081 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     1141 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     1201 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     1261 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     1321 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     1381 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     1441 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     1501 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     1561 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     1621 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     1681 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     1741 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     1801 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     1861 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     1921 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     1981 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     2041 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     2101 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     2161 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     2221 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     2281 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     2341 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     2401 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     2461 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     2521 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     2581 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     2641 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     2701 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     2761 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     2821 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     2881 ttacagcttt agatgctgta actatgggcc gtttgatggc gcgactatca aaattcaata
     2941 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     3001 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     3061 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     3121 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     3181 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     3241 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     3301 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     3361 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     3421 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     3481 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     3541 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     3601 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     3661 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     3721 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     3781 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     3841 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     3901 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     3961 aaatttggac agccgagatg tgggcacggc tttggaacat gatgtatttc aatattggtc
     4021 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     4081 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     4141 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     4201 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     4261 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     4321 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     4381 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     4441 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     4501 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     4561 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     4621 aactaggaaa aaagattggt gtagaagata cagaaatcac ttctttaaaa tttagaaatc
     4681 tttccatgag agatagtgat ataacagcta tggatgcgat gggatcgaaa ttaactaaga
     4741 aagtaaagac tccatttcat ccaatcgcca agaaatttaa taaagatcaa tattttatcg
     4801 taatcgatag tatgcgttac aacgttatct atgccgatta cgataatttt tatatctatt
     4861 tttatcttga aagtgtgggt gaatatggtg attgataatt ctaaagaaaa agaacgttta
     4921 aataagcaaa tttctgctat caaaacttcc ttagaagagc attttggcct caaactcttt
     4981 caagactccg taggcgagga tgagctacct gatgatttta attacttcat tctcgaaaca
     5041 ggagaaatag aaatgatcac tgagccaaaa tatagcgtgg gtcaaaatct atatctaact
     5101 ttctattcag aaaatagaga agatttaaca ggagactcac tagatattat ttcattgatt
     5161 caaaatcgtt cgattcgttt tcagagaatg gatcccaatc atttaaaact agagaaccaa
     5221 gatcgctata tcgatcaatt ggtatttacg tttagacgat tattgaagag tgattgtcat
     5281 ggctaaaaat agttgggagc taaaaataaa tggacatgat gaacttcttg tgcggatgga
     5341 acgctattca agcgagagcg aacgactgat taacgaagca ttgaaatcaa aaggttcggc
     5401 tattgcagtg gataggatta cagaaaaaat tcctgtttct gaagcagatt taagaagagg
     5461 gcaccaacac gcaaaaaata gtcgtccact taagactcaa tacattaatt tgggtttcat
     5521 cattagacct acaagaaaat ttgagtattt aaaatatcct gatttgggga taggtacttc
     5581 taaaagaaat cagccggacg aatttatgag aagaggatta ggtcttgcac ttgatccaat
     5641 tacagaactt ctgattcgtc aattcgataa attaaataaa tagggggaac aacaatggct
     5701 aaaacaaaaa ctgtagtaac aacgttcgat aacgtaagta tcaaacgaat tgcttttaat
     5761 tttaagaacg caggaaatgc aatcgcaacg aattgtaatg gacaattaga tggcgaaaca
     5821 gaaatgcaaa cggtggttaa aaaatgtgga gcgacagaag taaaatcaaa atctaaacca
     5881 atcaatatga cggtaacaat tactgcacat gtaccgatgg aagtttatcg acgtttcaat
     5941 gggttgaaac aagatggacg tattaaacca ggcatttact cttacggtcc tgattccgta
     6001 ggcgaagatt tctcacttgc tgcagagatc gtggatgact tcgaagaaaa tagaaagtta
     6061 gttggtatgt tagcatgcac ttcgaataca ggattaacat tctctattga aaatggtgcg
     6121 gatgaagtag ctgcgttaga actagaaaca aaagttatgc aagatgaatt tggtaaattt
     6181 tatcatgaag caattgttgc agaacttgaa gaagacttaa cagatcaatg gatgacgaat
     6241 ctatctgctg atgtgattaa aaaaacttca gttgtgacaa ctacggccac tcaatcacag
     6301 taaaaaaaac ggaggtagcg aaatgaacga agattactca aaaattgaac taaacgatgg
     6361 aacaattttg aatttagaac ctaaactgaa tatcaagaaa ttattgatga tcaatagaga
     6421 ttttaacaca gacgagtttg caaaaatgac tgtgggaaaa ggatccatgg atatttctgt
     6481 tattcaaggt gcaaaggctg tgtatattgc ttaccgccaa gcgaacatga ctgattatat
     6541 ttcattcgat gaatttatcg ataaatggga ttttgatatg gctactgcca gctatattta
     6601 tcaattgatg atgttcaaac aagcacgcga tgcttatcaa aaagaatttg aaaaagcaaa
     6661 taaggaaaaa aagcttcaaa agtaaaaatg ccaaagctct tagttgaaac gtgggtcgat
     6721 gtctattcga tgttgaccga cgttttttct atgccttcag atttggtttt aagcgatatc
     6781 tgtttagatg acattttgca aatggcttac aacaagagtg cttatgaagg atggaaaaac
     6841 tatgcaataa accaatctca gaaaaactaa agaaaggagg taaaaaatgg ctaaaaagag
     6901 aacagaagca gaagtaactt tcatagctaa cgatgacgga ttgaaatcta cgttaaaaga
     6961 aatcagtgct gaattaacta aaaatagagc agaattaaaa ctagaacaag ctcaattaca
     7021 acagactggt tctgaatcag acaagttagg aagtaaatta tcttctttag aaaagcagta
     7081 tgaattacaa agccaaaaag ttgaagtaac tagccaacgt ttagccaatg ccaaaaaata
     7141 ttatggagaa aattccaccg aagttcagaa acttgagaga gaactgatta accaacaaac
     7201 agcgcaacaa cgtttgtcaa acgaaattga taaaacgagt aatacactag ctcaagcaaa
     7261 aggcgaaata cagacgtacg agtctacaat gcaacagttg gatagtgaac aaaaaaatgt
     7321 tcaagctagt gcttctctga ttgaatccga atacaaaaaa tggcaagtaa ctgctggtca
     7381 atcagcttct gaatccgaga aattagcgaa agcccaagaa tatgtttctc aacaatctga
     7441 aaatgcagag aaaacgatag atatcctgag acgacagtta gaagctacac agtctgagtt
     7501 tggcgctaca tccacagaag caatgcagat ggaggcgaag cttaatgatg ctgaacgtga
     7561 atttgaagag ttaggacaag ctgctaaaaa tgtagataca actaacttgg acgatatcgg
     7621 aagcaaaata gatatgaata atttaatgga agcttctgac gttttaagcg acattggcga
     7681 taagcttaca gaattaggga aacaagcagt ggactctgct aacagtgtag gtagttccca
     7741 gagtaaaata caagctaatt ttggtttgac taaacaagag gctgaagaat taacgaatgt
     7801 agccagagac atttattata aaggttttgg agaatcgtta gatcagtcca cagatgcatt
     7861 gattttggta aagcgtaatt taggcgattt aaataatcaa gatttacaaa atattacgga
     7921 acaagctatg gtcctagaaa acaccatggg cgctgatatg gatgaaacgt tacgtggtgt
     7981 aaatggctta atggtcaact tcggattgag cgctcaagat gcaatggatt taatggtttc
     8041 gggtactcaa aacggtttag ataaaacgca cgaattaggc gacaatatgg cagaatatag
     8101 ccaattatgg agtcaaatgg gatattcagc tgatgaaacg ttcggaatgc ttcaaaatgg
     8161 tttagatgcg ggtgcttata accttgataa agtcaatgac ttagttaagg aaatgggaat
     8221 atcgttaaca gatggtcgat ttgagcaaaa catggatatg tttagtgaaa gtactagaaa
     8281 agcttttgaa gagtggaaaa atggtggagg aacacaaaaa gacgttatta attccatgat
     8341 tcaagatttt agcaatatgg atggtcaata cgaccaatta aataaagctt ctacaatttg
     8401 gtctgcactt ggcgaagata acgcgatgaa agttgtccaa tctttgactg atgttaacca
     8461 tacatttgat gatgttagtg gatctgcaca aaaaatgaat gaagattcta ctactccgtt
     8521 acaagaatta aacggaaaaa tagctgaatt aaaggattca ttagctccta taggcaacac
     8581 aatcatagat gcactcgaac cagtaattga ttttctagga aagatggctg atgcgtttaa
     8641 taatcttcca caaccagtac aggattatgc cgtagcgatt ggcggattga ctgctgcatt
     8701 tactttatta atgccaataa tagttggctt catggctcta ggtggtccta ctacattaat
     8761 aataggagca gttattactg ctattgctgg agttatagca attataaaaa actggggcgc
     8821 aattactgac tggtttaagg gaatatggag taaattcact gattggttgg gtggtacttg
     8881 ggaaagcata aaagaaggtg cctcatcagt ttgggatgga gtcaaagaaa cctggtctgg
     8941 atttgtagat tgggttcaag aaatttggca aggagtttct gattggtttg gagagttatg
     9001 gagcggatta gttgaaggag cttccaacat ctggcaagga gtccaagaga cttggcaagc
     9061 attcgttgat tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg
     9121 ggcagacatt gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc
     9181 tggtattaat attatcgtgg gtatctttga tgtagctgga cagttattag gcgcagcttg
     9241 gcaagctgtt tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca
     9301 atggataagc atcgcttgga atggaattgt aactacattc catactatat ttgatccagt
     9361 agtggcatgg tggaatggta tatggacatc tattagtact acggcttcaa atatttggaa
     9421 ctcaattagt gcaacagctt ctagtatttg gaatagtatc aagaatacaa tcactagctt
     9481 ggtacaagca gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg
     9541 tggaatttgg aattcaatca gctctacagc atcaaatatc tggagtagcg tgactagtag
     9601 tataagcaat gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc
     9661 ttcgtggatc agcggaattt ggaacggtat caaaaacact gctttgaatc tttggaatgg
     9721 aattacaagc actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg
     9781 gtccaatcta acaggtattg tttccgacat attcaataat gttaaaagca cgatagctaa
     9841 tatatgggaa ggtatcaaaa agactgttag cgctccgatt gattggatta gagataaaat
     9901 cagtagtgtc tttgataatt tgaatatttc tataccacat attccgttac cacattttaa
     9961 attgagcgga gaattcaatc cattgaaggg gaaaatccca acattgggtg ttgattggta
    10021 tgcgaaaggt agtgtgttta attctccaaa tattatcggt gtaggtgaat caggacctga
    10081 agcagtttta cctttgaaaa gatctgtgct gcaagaaatt ggtgatcgta tcttgagtag
    10141 cacctcagtt tcatctagga cacaagcggt tcaacctgtg aacaattacg aattcaattt
    10201 cacaattgat ggtaacgcag atgaagtcac tatgaagcaa acaactcaac aaatcattga
    10261 tagcattaca aaagttcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt
    10321 ctcctgtttt tttagtatta aaaaggatgt gaaaaaatga ctgattgtat acattctata
    10381 atcgatggat ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct
    10441 tctccaaaaa gacagagagt tgaaacttct gttttaggaa ggttaggtgg cttagtgcaa
    10501 gattactcgt ttgaagacat gtcgtttaca ttgcactata actatttaga ggatgtggaa
    10561 gaccatcaag cgttcaagca atcgttttat atcatgcgtc attggttaaa ctatgcaaag
    10621 aaattagaat tctctgacga tcccaacgtc tattacgtta tccagactat cgatattggg
    10681 gatgcagaaa acgatattgt tgaatgggga gagttcgatg taaatattac tgcgaaacca
    10741 ttcgcaagag ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg
    10801 ctgaataata gtttagaaga aagttttcca aagattatca tcactccttc agctacttca
    10861 tgccagttca tcttaaatga ttatgtgttt agtttcgaag gcttagtagc aggaactgat
    10921 gtagtcattg atagtgattt gatgctttgc tacgaagagc aatcggacgg agatatttta
    10981 gatcggtcca acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat
    11041 tttaattgca ctggtttgag caaaatacaa atttatcgta acgggttaag gtaggtgaaa
    11101 tagatgatcg ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa
    11161 catttatatg atacgcaagg tttaggcgct ttgtcagact ggttaacagc tactgttagc
    11221 aataaactaa acggagccga gatatttcag ggtacttatc caataagcgg aactaatgca
    11281 gatttgattg tagaaggacg tattattcag tgttatgtag atgaaaatcg agcaaaacag
    11341 cgtctacgga tttattatgc aaaaacttct gtaataggca atacgataga agtaaaagct
    11401 gaacctattt tcaatgatat aagaaaatcg gtgttgaata aatatgacag cggaacagaa
    11461 aaaatcactg ctactcaggc atggcaaaac gcaaaagctt tagcgaaacc agctatccct
    11521 tcgcagtttt ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaagcg
    11581 aattttttag aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggggaa
    11641 tttcttaaag ataataacac attacatcat gaaaaaaggc taggtacgga tcataaaatc
    11701 aaagcgattt atactaaaaa tttaactggt cttgacttag agatagatgc tcaaagcgtt
    11761 ttagttggag tttatccatt cattagcagt tcttcagaag gagaagacga gatcactcta
    11821 ccagaagaag tcattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt
    11881 gttgatttta aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg
    11941 ttgaaaacaa acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca
    12001 ttgaggcatc aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac
    12061 ggagtagatg tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat
    12121 acgtttaatg ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac
    12181 ttcttagaaa acacagagaa taatgtaagc aatttgatta atgatgccat tgatcaattg
    12241 aaaaatggtg gcgaaatcag tgatttaatc aatgatattg tagatcatca aactgatatg
    12301 attactggtc aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt
    12361 ttgattatgg acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct
    12421 ggtattggtt tttctaaaac tggcattaat ggaacatatg aaaccgcatg gacattagat
    12481 ggcggattca atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt
    12541 actacattaa ttagtgatgg cactgattat agaacaagta ttgctaatgg caaaatgact
    12601 tggtactcaa aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt
    12661 gctgatgccg gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat
    12721 ccacagggta acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct
    12781 tttggtgcgc ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt
    12841 tctttaagca ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact
    12901 atgaaattta ctagtgatgg tatgctaacg ttgccaggtt gtttttttgg ttcatgggaa
    12961 gatgggaaac ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt
    13021 agaggtacta aaaactcaac cgtaccaaca gaacattatg gacaacgact attaaacgct
    13081 tatgaaactc cagaatatta tttcgctgat tatggggaag ccgttacagg tgatgatggt
    13141 aaagttcgtg ttgatattga ccccatgttt gctgagacag taaatctaag tcgatatatg
    13201 acacatgtga cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat
    13261 ttcatcattg aaactagtaa gccaaacgta ttagttagat ggaatttagt ggcacaacgt
    13321 ctagggtatg aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac
    13381 caaaaacgtt tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt
    13441 tggctctaga tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcaaggtg
    13501 ataaagggat tacagtgact gttaaaccat ttttaaatgg cttgcagatg gatacgagtg
    13561 gcggaacatt tactttaaaa ggaacaacgc catctaaccg ttacgtagat agtgtggcaa
    13621 ctagtgtaac tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag
    13681 gatattataa acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag
    13741 atatcatttt tttctcacta ggagtgtctg acatttcgca aggccaagcc gatgaatacg
    13801 tttcgcaatt ggaagagttg attcaaaagt ataacgaaac ttttgatgct tttatggctg
    13861 aaatcgaagg tagagtggac agcttaaata aacagattac tgatttaact ggtcaagcta
    13921 aaacactaca agacaagtta gacgctctga aagaagaaat ttctaaatta ggtaacttgc
    13981 aggtgatgta cagtaacagc atcga
//
LOCUS       DANJJH010000075        13810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000075 DANJJH010000000
VERSION     DANJJH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(221..1423)
                     /locus_tag="PWS53_002098"
     CDS             complement(221..1423)
                     /locus_tag="PWS53_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872304.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="PWS53_002099"
     CDS             complement(1426..1620)
                     /locus_tag="PWS53_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0872305.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="PWS53_002100"
     CDS             complement(1667..2515)
                     /locus_tag="PWS53_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL0872306.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="PWS53_002101"
     CDS             complement(2720..3070)
                     /locus_tag="PWS53_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872307.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="PWS53_002102"
     CDS             3665..4111
                     /locus_tag="PWS53_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL0872308.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="PWS53_002103"
     CDS             4735..5727
                     /locus_tag="PWS53_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0872309.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="PWS53_002104"
     CDS             5743..6627
                     /locus_tag="PWS53_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0872310.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="PWS53_002105"
     CDS             6676..8283
                     /locus_tag="PWS53_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0872311.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="PWS53_002106"
     CDS             8284..9768
                     /locus_tag="PWS53_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL0872312.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="PWS53_002107"
     CDS             9945..10937
                     /locus_tag="PWS53_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0872313.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="PWS53_002108"
     CDS             10989..11858
                     /locus_tag="PWS53_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0872314.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWS53_002109"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="PWS53_002109"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0872315.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DANJJH010000076        13615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000076 DANJJH010000000
VERSION     DANJJH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            59..1252
                     /locus_tag="PWS53_002110"
     CDS             59..1252
                     /locus_tag="PWS53_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0872316.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(1418..1840)
                     /locus_tag="PWS53_002111"
     CDS             complement(1418..1840)
                     /locus_tag="PWS53_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872317.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            2423..2917
                     /locus_tag="PWS53_002112"
     CDS             2423..2917
                     /locus_tag="PWS53_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872318.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(3079..4251)
                     /locus_tag="PWS53_002113"
     CDS             complement(3079..4251)
                     /locus_tag="PWS53_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0872319.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(4457..5272)
                     /locus_tag="PWS53_002114"
     CDS             complement(4457..5272)
                     /locus_tag="PWS53_002114"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0872320.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(5422..5868)
                     /locus_tag="PWS53_002115"
     CDS             complement(5422..5868)
                     /locus_tag="PWS53_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0872321.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(5865..6869)
                     /locus_tag="PWS53_002116"
     CDS             complement(5865..6869)
                     /locus_tag="PWS53_002116"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0872322.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(6909..7238)
                     /locus_tag="PWS53_002117"
     CDS             complement(6909..7238)
                     /locus_tag="PWS53_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0872323.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(7364..9691)
                     /locus_tag="PWS53_002118"
     CDS             complement(7364..9691)
                     /locus_tag="PWS53_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0872324.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(10534..10827)
                     /locus_tag="PWS53_002119"
     CDS             complement(10534..10827)
                     /locus_tag="PWS53_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL0872325.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(11230..12408)
                     /locus_tag="PWS53_002120"
     CDS             complement(11230..12408)
                     /locus_tag="PWS53_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872326.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(12512..12826)
                     /locus_tag="PWS53_002121"
     CDS             complement(12512..12826)
                     /locus_tag="PWS53_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872327.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            12933..13151
                     /locus_tag="PWS53_002122"
     CDS             12933..13151
                     /locus_tag="PWS53_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872328.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(13146..13541)
                     /locus_tag="PWS53_002123"
                     /pseudo
     CDS             complement(13146..13541)
                     /locus_tag="PWS53_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3902 a         2603 c         2432 g         4678 t
ORIGIN      
        1 ttcttttttg gatatacttt taacaattta gggaaatttt aggaaaaggg gtataaaaat
       61 gacaacgtat cagcgaaagg tattagtatc gacatcggcg ggaattgctt tagagaatat
      121 ggatattatg tttttagctt tttcattatc ttcaatgatt acttcttttc atattagtgg
      181 tacgcaagcg ggtttcattt caacgatcac taatttgggg atgctcattg gaggtatttt
      241 ttttggcatc atagctgata aatatggacg tgtgaaagta ttttctcaaa cggtccttct
      301 gttttcgatt gcttctttat tgatgtattt tgcttcaaac atctacttgg tttatctctt
      361 tcgctttatc gcaggtgtag gagcgggagg agaatatggg gcgtgtatgt ccttgatttc
      421 cgaaagtttt tcaaaaaaac aaattggacg agcttcttcg gttgctggta ttggtgcgca
      481 ggtcggtgcg gctttggctg cgattttagc tgctgtgatc attccaacgt ttggttggaa
      541 gatgttgtat gtcgtaggtg tactgccggt gttgatggtc ttgttcattc gtcgcggctt
      601 gactgaacca aaggaattca aatctgtaca atctgccgaa aagaaaacca aacttagcag
      661 actgtttgaa tcaaaacatc ttgcttggca aacatgtggc cttagtatca tggtaactgt
      721 ccaaattgcc ggctattttg ggttaatgaa ttggctacct tcaattatgc aaaatcgatt
      781 agggctttcg gtttctgggt cttcattgtg gatggtcagt actattttag gcatgtcttt
      841 ggggatgatg acttttggca taatcatgga taaattgggg cctaaactag ccttttcttt
      901 gtttttaatt tgttcatcct tatctgtcta tctacttgtg ttagcccata gccagtggag
      961 tttggtagca gccgctgtga tagtcggcta ctttatcaat ggcatgtatg gtggatatgg
     1021 cgcagtgatt agtagtttgt atccaacaga aatccgcgcg acggcgaata atttcgttat
     1081 gaaccttgga cgagctgttg gtggtttttc ttctatcgtc atcggtttct tgatggatca
     1141 ttatagttta actgccgtaa tcttgttttt gagcggtatt tacctagtca gtctgctggt
     1201 cttgacaact cttactggtg ttacagcatt aagggaaagc ttgcagcatt gatagttgag
     1261 tctgtaaatg acaagaaaga ggtgtggaaa aagcttgcat ctgcggtaat aagaccagcc
     1321 aaatcaaaaa catgagacac tgtttccgtt tggctggtcg tattacttga tgcagggcag
     1381 ctttttcaac tgctaatttc cttctatctg gcggcgacta ttctttgacg tccgtcattt
     1441 caaagtactt tactaataac ttagcataac gaaaaactgt ggatacactc gtatattgag
     1501 cgtctgcata atcttccaaa tttttctcag gatgttgata catgaattca atcaaatgat
     1561 aattatatga ttttctgtag aaatgaagat aaataatgta gattgggaag ttctcctgct
     1621 tttccataga aaagcgccga ttattctcat tcttcaaaat gaccttatca ggatattgtt
     1681 ggaacaattc ttctaaatct gttagataat tcaagataga cttacttgat accttcaatt
     1741 cgttttgtag ttcttctatt gaacaccaac gttctgtttc aagcagtaaa tgaatcattt
     1801 taattttctt ttgagaaact tttgttaaaa aagtctgcat attttctcct cctgtatcta
     1861 ctttactata ctacatacaa gaaaaaaacg gatacaaata tcatattgtc acaccgtcaa
     1921 tagtgcataa acctgttatt atttctcttt ttttcctaag tattctattg atatgaatca
     1981 tttaatcgtt tattatataa atgtcaataa tctaataata aaataacgta catattttat
     2041 tattgcatac ttaataacag aactatagca aaaaatcccg tgtatcaaca cgggattaga
     2101 ttaagtttcc tcaatcacta ttcataacac aattattata acaagtaagt aaacaattga
     2161 aaaggacatt tattgaaaac ggttgttttt tacttaagga ggaaatttag tgaaaaaatt
     2221 agttgcgttg tttttggtcg caggaagttt gttgagtggg tgtggtgcta ctacgtctac
     2281 tactcaaagc acaagttcag ctcaggcttc cagcagcagt accacgatct ccgaaagtac
     2341 ggcagattca tcgaaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga
     2401 aaaagctgca ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc
     2461 aaatcctaca aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa
     2521 caaacaagag ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc
     2581 agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca
     2641 agaagcaatc gcacagcaac aagcagaaca ggcgaggcaa gaagcaatcg cacagcaaca
     2701 agcagaacag gcgcgacagc aagcagcagc tgaacaggct tctcagcagg cacaagccgc
     2761 agctcctgag caaaataatg aacaaactgt ctatgtcaca ccgaccggtt caaaatacca
     2821 tacacataaa tgtggaaatg gtacgtatac ccctgccact ttaagcgaag ctcaagctcg
     2881 tggtttaacc ccttgctcca aatgttatgg aaactaattc aaaagttata ctataataat
     2941 aaaattcttc tgaaacataa aaaaatagaa gctggcgttc cagatagtaa accaaaaata
     3001 gcagtttaca cgtccatttc gtcagcttct ctcttttttc atttagtctg ttccctctgc
     3061 ttttttggac atcttcattc attctttctc aatcaaatag tacacctctt tttcttttcg
     3121 cagtttatat cttccttttt tcaatttcat tgaaaactgt ttcgaatgtt tatgttttac
     3181 atggatttta agcaggagct cctcatgttg caagcgatag ttcctgcctt ttaaatcaga
     3241 aaatctcact agtccgtatt catcagaagc tttcacaaat ggaacttttt ctctagtaat
     3301 atagtgtttg ccgttgagcg tttttacaga aaagaccatt ccttcgattg gtcgtccgtt
     3361 tgtgttcttt acataaaaag ataaaggata gtttcgctta gcgatggcca aagaatctaa
     3421 ataagcgatg agtagccaaa ctaagccgat tgatccaagt agtataccga taaaccaaat
     3481 aaaaaatttc cattttttcc attgatcaat tccttggatc gctttttttg cctcttttgc
     3541 ttgataagga atacgatgcc ccgtcacaag cagtcgatgt gtattgacca tataaggagt
     3601 gcaggtaagc aaagtcacga gatctctgcc tttttggata cccaaagaat ctacttcgtc
     3661 aggtaagacc acttggattt tttctacttg atacgccaat gtctttccat tgatctggat
     3721 gaaaaactgg tctccttttt tcaacttagg caaatctgta aataattttg cttctgggag
     3781 tccacgatgc ccagaaagaa cggcatgggt gtttttcccg ccagtcggat aagaacttcc
     3841 ttccaataat gacgttcctt tttgcaaaaa tatttccgtt gtctgatcga atataggtaa
     3901 gctgacattg atttttggga tttcgatgac tccaagcata tgttccataa aaaaggcatt
     3961 actaggtaaa tcttttaatg cttttgcatc aactgcttgg ttgaaactgc ctatccctgg
     4021 tgatacgttt tgctctgcca actgttggtt tttctttgtc attttttcct gctgtttttt
     4081 tatttcctct gaattcttat tcgatgcttg cttttgataa tggatgatca tttgctgggc
     4141 aagaaaatca ttcagtgaat cttctacaaa aggataagaa aagatcccga tcccaacgac
     4201 taaaatgatt gccatgaaga tgtccagtat tcttattctg cttttattca tcatgtccac
     4261 ctattttcta atatgtatat gaagaggttg tgaaatcagc gccttgacct gagtattagc
     4321 gaagcaaacg gcaaaatagt tcctcctatt ttgccgtttg ttgagcgaat accaagagac
     4381 cgaccacttg tcacaacctt tctccccatt tgaatcacta tcgtttattt atcgtatgat
     4441 ttttgtttcc aatagtttaa gcttctgttt cttgatggcg ttttttgaaa tagatcactg
     4501 ctcctccaac taaagcagta cctaccaaga tgaatacgat gatccccata ccaccagttg
     4561 aaggcaagat tcctttaggt tggttatcga tcgttgtttc atcagaagtt gttggatcat
     4621 catctactgt aaattcagtg tacgcagctt cattcttagg taatgcgtag ccttctggtg
     4681 cttttgtttc ttgaaggcag tatgcccctt gtttcagccc ttcgattgaa aatttccctt
     4741 ctgaatcaga aatcaatttc acttcttccc agtcagaacc tgtgacttca tctgaccaag
     4801 catatgggta ttctcctgag gcaaggtttg ctattttttt cattaattta acagttggat
     4861 cttttgctct tagaatagaa aattctgcat cggctaattt ttcttgcgtg ttcacatctt
     4921 gtttgatgaa gtggaaatca ccgtagtttt ggacattttc tacagtaatc gtgttatccg
     4981 tagttgtatt ttcccgattg actacaccgt ttggattccc aagattggct tcatctgcgc
     5041 catggatgat accggttgtt tgttgcaatt ttccgttttt tactataaaa gtagtttctt
     5101 tttgtggatt ggttaattga taaccatctg gtgcttttgt ttcaactaaa gtataattgc
     5161 cgtctattaa tccattgaca ctgatggatc cttcagtatc agttgtgaat gtcaacgctg
     5221 actcagcagg atcatagaga gcgacaccag tgttttcatc aaaaccggtc acgaattgat
     5281 tctctgaatt tttgatctgg aattgcgccc ctgctaactt atctacccct gaaaagttcc
     5341 cagctttctg gattttgata ttgctttctt tgatgacatt tttagcatat aggtgaatcg
     5401 tgtctaaagg tttgtcaggg tctagggtaa gcttaacacg atatttggcg acgtttcgac
     5461 accgggcttt ggtgtttcta caaagacgta tacagcatct ttcccatcag atttacctgg
     5521 aagagtcagg ttagctaatc cttcaccaga agtcgttaca cttgcgaagg gtgtagcgtc
     5581 tgctggaact gctgtgtctt tacttggata gtacgtagca gtgatgtcat atacgttgaa
     5641 ttcaacgccg ttcaacggtt cgctattcgg aaatgtcaat tccgttccgt tgtttatttg
     5701 attttctggt gctgaagtga atttcaattt atgcaaatta accgtaacat tttctggtgt
     5761 aggtgatacg gctgcagaaa cctctgcagt tcctaggccg agaacgaaag gaagcagcat
     5821 gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt tatttccctc
     5881 ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga aaggactagc
     5941 aataacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg aaagcttcct
     6001 ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt tccattattc
     6061 gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag cggatcataa
     6121 ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc tttaggcggt
     6181 ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa tgttccatct
     6241 ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg atttatcccg
     6301 ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc tgctagtttt
     6361 tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat ggcaagatgt
     6421 gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt cgttttttca
     6481 ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt tttttccagc aaacgaacta
     6541 agatcttttc cagaatctga taagacaaaa ccatgtttta ttttttgtgt gtgaaatgta
     6601 tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca taactgagga
     6661 tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg aattgttacg
     6721 gtgatgagat aagggacttt atccccatct gcaaaatctg tctgcttatt ctctactgtt
     6781 tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa gtggatggtc
     6841 gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg tgctgatttt
     6901 tcttttactt aatctgaagt ctttatttcc gtaaatcggt aaacagcgtc acgaccttgc
     6961 tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac tgtttttgtc
     7021 acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct ttgtgcgtct
     7081 tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc atatatagcg
     7141 aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt tattccgtcg
     7201 ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa agtaaatgta
     7261 ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag taggataaaa
     7321 aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc tgttcacctg
     7381 cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc agtcctaaaa
     7441 agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc ggattgatcg
     7501 tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt ccttcgccta
     7561 aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt agggtataat
     7621 ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa atcgagtgac
     7681 tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta gctttataga
     7741 tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga tttgctgctt
     7801 tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca ttaaccatat
     7861 tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact tgtactggag
     7921 taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca cctgcttttc
     7981 catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta atcgtataga
     8041 catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt ttagctagtc
     8101 cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca gttacttcaa
     8161 ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga tcaatactga
     8221 gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat ttaacgatcg
     8281 gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat actgttgcat
     8341 ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc tttacgatta
     8401 catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc tttgtttcta
     8461 tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc gtcaggtctt
     8521 gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc tgtccaaaat
     8581 agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc gtatcgacgt
     8641 tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc gttactgctg
     8701 ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc ccgtacgttc
     8761 cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga ttcgcattga
     8821 cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta ccatcaacga
     8881 tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac tgtataccga
     8941 ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag ggtgtaaaag
     9001 aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg gtagccaccc
     9061 ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag ccaataaaca
     9121 gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg ccttgtaacg
     9181 cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct ttggcatata
     9241 tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc gcaacagtac
     9301 taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct tttgctgctg
     9361 tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag gttgtctcgt
     9421 tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt ggactatttg
     9481 tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt acataaatac
     9541 ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg ggatgtaagt
     9601 attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat atacgttgga
     9661 ttttattttg agttttctta atcattttca tccctcctct caataaaaaa tcatcaaata
     9721 atcctttaca cgttattcta aatttaattt ttattatttt attgatgtat agaaagtttg
     9781 gggctttttt tctcttttta ttctaaggtt attttagtct tgtcctttaa tacaaagatt
     9841 ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg
     9901 attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg
     9961 ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa
    10021 tgtgtcgact gcagtgttgt gtccctcatc acgaataaac ggtgttcaat tagctgatct
    10081 tgtattcact taaaaaatgg taaagtacga gttgctattc cccattttca attttctaac
    10141 gctcaggaca aaatactgtt ttcacaacct cattggaata aaagcatata tattgtttca
    10201 agaacgaaat cttgttatct ttttaaaggg catctgtcca aaatctaatt ttaggaggta
    10261 cgttttggta tttattcgta caaataaaag agaattatct ttattcatgt aacgcatcca
    10321 attgattttt tcatgtacgc tcctggaacg attttactga cgattgctta tttaactgcg
    10381 aatcgctcat tagcttttat catagctatc catattttga ataacgttct tggctttgta
    10441 ttatagatac agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat atcgcatact
    10501 tgaacttttt tcaatgagta taaattaaaa cttctaattt tcaattccca tcatctgata
    10561 tgcccgaccg tctgaacttc ttttgaaaaa gccaaatgtg attagataac gtctttgttc
    10621 gacataatcg ccactagaaa atacgttcag cagtatcgaa ttaacctctg tttctgtata
    10681 agtcactcca tggtcaaagc gtttcaagac ttctttcaat aggatgtatt tcttttttgt
    10741 tgaacttggt acttgtttta cttttccatc attcatgaat gtgtctaaaa ctttcatctt
    10801 ttcttgctcc gtataagtat aaaccatctt ctttctcctt ttgattctaa gaatactcca
    10861 tttatgttag aatattctgg attttttatt aaagcggtta ctcttaagaa agatattatc
    10921 atagtgctct tttttttact actacttata ggtagattta ttctaatcca ttattctccc
    10981 ctaccaataa ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag
    11041 aacccttgaa taccaagggt tctttctcag tctgataaca atgattaacg acggatttct
    11101 ttgatacttg tgaaaagtcg ttaaagtatt gatataaaag gatttttatt acctctgact
    11161 acatattgac taatttgtta aaaaatcgtt tttcaagaat cctgtaatac cagtgctttc
    11221 accgtttttt taactctcta aagaactaaa agcaatcaga ggttttacct tttcatttga
    11281 cgttaagtgg cgataaattt tcgaagttgt ttggtcagta tggcctaagc gatctttcaa
    11341 gacttcccag ctacagtgtt ccgctactaa gcttgccatt gtatgacgag ctaaatgagt
    11401 atgaaatggc ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat catttacata
    11461 ctctcgacga taaggaacac cacgtttact agaatcaaga tacacaaaca aatatgtttc
    11521 ttctataata aacctgtaat gttgcctcat ctcacccaaa gccttaatca actgttgtag
    11581 cttttccata actagaggag ttactagaac tcgtctagca gaactaactg tcttagtagt
    11641 ttcaattcga aaatccttac tcttaccttt aactgccgtt tgcttataga acagaatttc
    11701 atttcttttg aaatctatat ctccaggctg tagagcaagc gcctcgctga ctcggcagcc
    11761 agtgtaaaat agaacatgga aaagtacaga catcggtaat tcctcatgaa caatgccata
    11821 attcaaaaaa gttctcaatt catcgacagt aagaaacttg tcctcaatat tatttatggc
    11881 ttcttttaaa tcagttaccg ttaactgata ttttggcatt ctcacagact tcattggatt
    11941 ttcacttatc aaattctttc tttgacaata tttaaaaatc atatttagaa cttgaataag
    12001 tgattgtaaa ctatttttgg catagccaac atttgttcca ttaacgccac attcctttaa
    12061 ggtaaaaagc aattcttgaa ggcactctgg tgtcaaatca gacaactttt tatcaccaat
    12121 ccatcgaaca ataattttta attgttcctc tcgcttatca taggttattt ccttttgttc
    12181 atttaacgaa cggtaatatt ggaaccattg ctcacctaca acactaaact tgtcattgct
    12241 tgcaacaatt tttttcttaa gcctttgttg tttctccatt tccagataag cgagttgcgc
    12301 ctcacgtttt gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt accatgtgat
    12361 cctaccagta gaactgattt gcttcttgaa tcgatctgtt ttttccattt attgattcct
    12421 cctattttga aggaacccaa caagcagaaa ccctttggaa atctttctat ctatgtcatc
    12481 attgtaccgc atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt cgcaattttt
    12541 ctcgatagtc attcgaatca ctctttctca tccattctaa aacatcatca cgaaaatagt
    12601 gtcctcgaat cccgtaccaa cagggaggaa actcaccaag ttttactaaa cggttcagtg
    12661 tactatcact tatctgaaat agtcgcttta aatcagtatt tgttaatacc agaggtaaat
    12721 gactgactgc aagcaatttt tccgtgtaat cttccagttt tttttgtaaa attgaataga
    12781 actctatttc cagagaatta acatttgttt taacttccaa ctccatcacc cttctctctc
    12841 aaaatcaaaa cgaccaccac ggtacccctt aaaattgtaa aaaaacactc cagtactcca
    12901 tcaaaacgga ctagaaaaga ggaattttta atatgaaaca aggaattgca gacataaaaa
    12961 taattaaaga aattcttgaa aaatctacgg ctaatgcaat agcattcgga acaggaatca
    13021 acttgagtac agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa ttaaatcttg
    13081 ccgatgctat taaaatcaca gaatttggca tgaaaaatat gccaaccaaa atcgaaatat
    13141 ggaaatccta gctcttccaa agccatttgt cgttggtacc aaacactgcc gagacttttc
    13201 ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat aaaaatgatt
    13261 tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt ggaaataaag
    13321 attttcatac caaggacaat aaaagtacca ctactattca aatttggttc caaataatag
    13381 aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg ttggcgaaat
    13441 ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt acaaaaactg
    13501 tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc cccctcaaaa
    13561 aattcattca actcgtttta cttctatccc tttccattat tactacaatc tagga
//
LOCUS       DANJJH010000077        13395 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000077 DANJJH010000000
VERSION     DANJJH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(14..706)
                     /locus_tag="PWS53_002124"
     CDS             complement(14..706)
                     /locus_tag="PWS53_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0872329.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="PWS53_002125"
     CDS             complement(1143..1712)
                     /locus_tag="PWS53_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0872330.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="PWS53_002126"
     CDS             complement(1786..2109)
                     /locus_tag="PWS53_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872331.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="PWS53_002127"
     CDS             complement(2263..3075)
                     /locus_tag="PWS53_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872332.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="PWS53_002128"
     CDS             3429..4277
                     /locus_tag="PWS53_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL0872333.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="PWS53_002129"
     CDS             4422..5363
                     /locus_tag="PWS53_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL0872334.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="PWS53_002130"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="PWS53_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="PWS53_002131"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="PWS53_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL0872335.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="PWS53_002132"
     CDS             complement(9827..12013)
                     /locus_tag="PWS53_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL0872336.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="PWS53_002133"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="PWS53_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL0872337.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="PWS53_002134"
     CDS             complement(12235..12675)
                     /locus_tag="PWS53_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL0872338.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="PWS53_002135"
     CDS             complement(12749..13288)
                     /locus_tag="PWS53_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0872339.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3763 a         2807 c         2380 g         4445 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DANJJH010000078        13283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000078 DANJJH010000000
VERSION     DANJJH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            415..621
                     /locus_tag="PWS53_002136"
     CDS             415..621
                     /locus_tag="PWS53_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL0872340.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(823..1824)
                     /locus_tag="PWS53_002137"
     CDS             complement(823..1824)
                     /locus_tag="PWS53_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0872341.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1829..3742)
                     /locus_tag="PWS53_002138"
     CDS             complement(1829..3742)
                     /locus_tag="PWS53_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0872342.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3910..4416)
                     /locus_tag="PWS53_002139"
     CDS             complement(3910..4416)
                     /locus_tag="PWS53_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0872343.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4576..5016
                     /locus_tag="PWS53_002140"
     CDS             4576..5016
                     /locus_tag="PWS53_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0872344.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5175..6515
                     /locus_tag="PWS53_002141"
     CDS             5175..6515
                     /locus_tag="PWS53_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0872345.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6584..7741)
                     /locus_tag="PWS53_002142"
     CDS             complement(6584..7741)
                     /locus_tag="PWS53_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872346.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7744..8100)
                     /locus_tag="PWS53_002143"
     CDS             complement(7744..8100)
                     /locus_tag="PWS53_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872347.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="PWS53_002144"
     CDS             complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="PWS53_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL0872348.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9568..10407)
                     /locus_tag="PWS53_002145"
     CDS             complement(9568..10407)
                     /locus_tag="PWS53_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0872349.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10592..11479
                     /locus_tag="PWS53_002146"
     CDS             10592..11479
                     /locus_tag="PWS53_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0872350.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12063..12758)
                     /locus_tag="PWS53_002147"
     CDS             complement(12063..12758)
                     /locus_tag="PWS53_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDL0872351.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12742..13140)
                     /locus_tag="PWS53_002148"
     CDS             complement(12742..13140)
                     /locus_tag="PWS53_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0872352.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3980 a         2665 c         2055 g         4583 t
ORIGIN      
        1 attatttttg tttcttattt tctaaaaaat taaaaaaaat tttggtgaat taagtatttc
       61 cttgataaca ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt
      121 tgatatgatt gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc
      181 ttttgggggt atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga
      241 cgtatgatgg taaactttgt acctatctga ttgctgaatc attaattgct tatcattagc
      301 catctttgtt tcaagttcgc tagatgatca ttttattcac atcctaacaa acttgtcaca
      361 tttatagatt cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa
      421 gctatccata tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt
      481 tgttcccagc cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta
      541 ccactcggct ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa
      601 cgatcaaaac aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg
      661 tgtgatattc ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac
      721 aagttacaat attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg
      781 gagctaagtg acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat
      841 tactaacttt tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg
      901 gttcaaaaca gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc
      961 aaatacagaa gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa
     1021 gaatgtgttt tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc
     1081 tgattgttca aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct
     1141 agagatttta tcacttactt gagtaagtgc agaaggatcc aacagaccag acaaagaaat
     1201 aaacttcgta atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc
     1261 ttcattttga agtgctaaca aatattctcc actaatttgt gcacctgccg aatttccacc
     1321 aatcgaaata ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc
     1381 ttcgttcaat tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat
     1441 atttacgatg atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt
     1501 tttatcccct cctacatatg cgcctccatg catccagaaa agaatcggta attttttctt
     1561 ttccactttc ggtgtgtaga tatccatatt attatttttg tactttgaag gataggatat
     1621 ttcttttttt atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac
     1681 ttgttctttt tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt
     1741 gatttgatta atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat
     1801 cactattcgc ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga
     1861 taatcgtgct aacagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc
     1921 cagttttcgg caacattctt ttttttgcat ctgtcttttt ttgtactttc actgtcatct
     1981 gaatcggttg cgtatcatca cttgattgat cctctcgagg ttcattcttt cccttattgt
     2041 ctgcattctc tgtcggatct tgcttttttg gtggagctac tgggattttt ttgtaataga
     2101 aaataatagt tggtatcgtc tcattaaaaa tcccttcagt cggtccttca acatttgcta
     2161 attcgtatcc ttcgaatgtc ttttgctctg tcatataatt ttctccataa gctccgtaaa
     2221 gctggatttc gtcctggtct ttgagtagac ttttattgtt ttcatccaca tactttacgt
     2281 ttatctgatc atggaacact actttcaaaa agtccgttac atgtttcgtg taggcagcat
     2341 ggtcaacttc ctgtgccttt ccatgccctg ctccttctac taataaaaga tctttatacc
     2401 ctcctgataa ctcatataat ttttgcgcgt tagacacagg tactacatca tctgctgttc
     2461 catggataat caatttaggc aatgaatctg acattccaat cgtaggtaga gcagcttttg
     2521 tatccaaatg catctcctga tcaaaatagt attgtttcaa aaggcccata aataaatctt
     2581 catgctcttg atccccaatg atgtctaact gattttttat ggcatccggt agtgcggggg
     2641 ctactgtctg gctgtacgta aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt
     2701 caataattcc tttgacattg acaggaatat ctttcgctaa tacagacatc atcgtagctg
     2761 ctcccatcga accaccataa agtaaaatct tttgagatgg ccgctctcga acttcttgat
     2821 ttatccagta aaggacgtca tctgactcat attgtccata ggtcacataa tcgcctccgc
     2881 tattaccagt cgctctggaa tcaacaaata ataaattata cccggcctta taaaaatcat
     2941 tgtattcttc tgtaacgatc ccgttattcc aattccctcg aaatccgcca tggatcatga
     3001 ctgttttatc tgaaccttga tcgacataat atgcatatac atccatcatt ttgccatctt
     3061 gtttgtttag tacagtttga tgaagttgtt ttgtatttgg atagaagcct gtgttttctg
     3121 taggcgttgt cccatcatct gtacctccaa gcatcgacat caacacactg ttttttgcaa
     3181 aagcgatatc gtacaataga taagtagcaa tcagatgttt gataatctct ttatctacac
     3241 cataatcatt aacagcaagc tcagtgacta ataactctat taattttgac ttgatgtctt
     3301 ctgttggtcc attaagtatt tctgtcatct gtttaccgaa gtaattcaaa tattctttac
     3361 cattgaaatt ttcaccttta ccgatatcat cattttctgt cccattctct ttgtcactta
     3421 ctttagcaga agaggattcg tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt
     3481 ggttatctgt ggtagcggtc gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg
     3541 tttcagtatt tgtttctgtt tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag
     3601 tttctgtggt ttctacctcc cgagattccg attcggaaga aatgctctct ccatatactg
     3661 tccattgcgg gacgcttgta cttacagcaa ataagatgac caacgctccc tttaatactt
     3721 gttttgttct caagctattc actccttttt atgtttctag gagaattata tgtaaacgca
     3781 aacatttttt atacaaaaaa taaatggttt atcccataaa aatagaacag gattattttc
     3841 tcattttacg taataaagca gaatggataa accagtatcc attctgcttt ttttaaacga
     3901 agaaacaact tattgctcca aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa
     3961 agcgattgtt tcctttgctt tcataaaagg attatcataa gttgtcgttg ctcctctaca
     4021 catcataagc tggtatccta atccatgagc aaacttcaca gtcgtatcta tacaatactc
     4081 tgcttgtgcc ccacataatt cgatcgaatg acagtccaat tcagttaata aaacactcaa
     4141 attagtctta taaaatgaat tcgcatgggt ttttctcaca aaataactgt gctcaggaat
     4201 agataattcc gacaatagct cccatggttt tgtttgtggc actaattgag catcttcgtg
     4261 ttgaacgaaa ataactggtt tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa
     4321 tgctgtcact gtttctaatt cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa
     4381 atcgatcact actattgcgt cggctgtgtt tgacatactt gtgccttctt tcttttttct
     4441 tgatttaata gtatttctta tctagctagt tccattatac gtgacaacat tttcttattt
     4501 tacacctcta aatggcactt ttcgactatt tcttcatgtt attatttcat aaatacttag
     4561 ctattggagg aatgtatgat gattaaattg tatacaggtt ctggctgctc gtcttcaaga
     4621 cgcgcaaaac gttggcttat cgtccatgat ataccgttta tcgaacagaa cttaattcgt
     4681 caaccactaa ccaaaaatga atttatccac ttactaaaat tgacaaacaa cggcgtgacc
     4741 gaaatgctct ctactcattc tattagttat aaaaatcttg ttcacagaat agatgaactc
     4801 agtttgagtg aactatatat tgagattcgt acatcccctt ctttattgaa aaaaccgatt
     4861 atctacgacc aatctgcaaa acttttgact ggatttagcg aagataaaat caggatgttc
     4921 ctttctaaaa ctaaaagaaa gcaagagctc aacgaactct tgctttctgg tcatcttgtc
     4981 gattttaaag aaaataatct ttttgcaatg tcttaatttt ggcataaaca atctgctttt
     5041 ctggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     5101 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
     5161 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     5221 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
     5281 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
     5341 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
     5401 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
     5461 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
     5521 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
     5581 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
     5641 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
     5701 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
     5761 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
     5821 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
     5881 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
     5941 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
     6001 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
     6061 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     6121 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     6181 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     6241 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     6301 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     6361 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     6421 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     6481 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     6541 ttgaatttct tcaccaacac tcattgacga agagccgctt ttcttaatta acgaaatcca
     6601 atttttccaa tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac
     6661 gcgcccaatc attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat
     6721 catatgcctt tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg
     6781 atgcactata ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt
     6841 cctgtgtttt accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa
     6901 aaatatccct gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag
     6961 tattaagctc tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca
     7021 tcactggaaa tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct
     7081 tcttttttgt cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat
     7141 aatttttttc tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag
     7201 acgattcaat ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt
     7261 ccaaataggt gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga
     7321 tgtgattcat cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact
     7381 cttcttttgt tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg
     7441 agtggccttg tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat
     7501 ctatactacg cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt
     7561 ttatacttga taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa
     7621 aaaagtatcg gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag
     7681 ataaatgata gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca
     7741 aacttatata attttttgac taagattttc taaggattga taagtacctt tgtaaagtga
     7801 atccagtaaa atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa
     7861 aaactgagaa gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata
     7921 ttctggatac ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag
     7981 ggtgtagttt agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata
     8041 acaatagttc aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa
     8101 agtacatact cctttttatc tattatttag acttattccg acagctacat gaataacgta
     8161 caaatagatt cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt
     8221 attcttgctc ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt
     8281 aaacaaaaat agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga
     8341 aatattcact caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta
     8401 tttcactcga tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac
     8461 acccatccat gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt
     8521 tctattatct gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg
     8581 ctgttttagc acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat
     8641 gataggtacc aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct
     8701 tcctgtgcta ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt
     8761 actgctttcg ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     8821 ttcgctgctc gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc
     8881 gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac
     8941 aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac
     9001 aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat
     9061 tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac
     9121 tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta catcactagc
     9181 attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac
     9241 aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt
     9301 actgattcca atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc
     9361 tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa aggaattata
     9421 taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg
     9481 tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac
     9541 cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg
     9601 ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt
     9661 tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat
     9721 cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc
     9781 ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta
     9841 gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat
     9901 gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa
     9961 cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc
    10021 ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga
    10081 atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat
    10141 tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata
    10201 atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc
    10261 taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc
    10321 cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga
    10381 tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac
    10441 ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat
    10501 ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt
    10561 cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact
    10621 aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga
    10681 aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa
    10741 agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga
    10801 tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa
    10861 aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata
    10921 taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt
    10981 aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat
    11041 cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaagggga
    11101 aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg
    11161 aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt
    11221 taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca
    11281 gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa
    11341 atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat
    11401 ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt
    11461 aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag
    11521 tgatttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg
    11581 ggcctttttt tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa
    11641 tttaaaaaag ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca
    11701 tgcttcccta ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact
    11761 cgactgaaaa gttattttaa aaaagactaa aaatatccct tctggacttg ttcctcttat
    11821 tctgttatac tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat
    11881 gaaatttatt caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa
    11941 tacatcgttt aaatgagaaa taagtaatag tcacccaaac tttaaattaa tgaatattga
    12001 atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat ggagaaattg
    12061 attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg gaaagtttgc
    12121 acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc taatacttga
    12181 tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc tgtagtgact
    12241 tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt acgttcgatc
    12301 tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg catcctgtta
    12361 taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg taattctgtg
    12421 taaatagctg taccggtaat caaataacct tcttttttca actttttgat cgcttgaagg
    12481 cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc tatgataact
    12541 cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg gataggaatc
    12601 tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc tccttctttt
    12661 ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg acaagaataa
    12721 atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc cattctgatc
    12781 ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct tatcttctac
    12841 atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg tgatatgtcc
    12901 taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat acatttgttt
    12961 atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca attcaatctt
    13021 ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca ttattttttc
    13081 tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa agatcgtcat
    13141 ctctgttcct cctcgtaaaa tatcatttgt ctaatagttt ttattcaaaa tgtttctaga
    13201 ttataaaatt tattgaataa cacattccta acggaatttg tttcgatatt gtgtgatttc
    13261 tcagagtaca atggatgtat gga
//
LOCUS       DANJJH010000079        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000079 DANJJH010000000
VERSION     DANJJH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWS53_002149"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="PWS53_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0872353.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="PWS53_002150"
     CDS             complement(322..891)
                     /locus_tag="PWS53_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL0872354.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="PWS53_002151"
     CDS             1077..1979
                     /locus_tag="PWS53_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0872355.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWS53_002152"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="PWS53_002152"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL0872356.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWS53_002153"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="PWS53_002153"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL0872357.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWS53_002154"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="PWS53_002154"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL0872358.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="PWS53_002155"
     CDS             8547..8732
                     /locus_tag="PWS53_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL0872359.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="PWS53_002156"
     CDS             complement(8794..9990)
                     /locus_tag="PWS53_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0872360.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="PWS53_002157"
     CDS             complement(10160..11596)
                     /locus_tag="PWS53_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0872361.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="PWS53_002158"
     CDS             11889..12698
                     /locus_tag="PWS53_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0872362.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DANJJH010000080        12470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000080 DANJJH010000000
VERSION     DANJJH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            10..483
                     /locus_tag="PWS53_002159"
     CDS             10..483
                     /locus_tag="PWS53_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL0872363.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            492..719
                     /locus_tag="PWS53_002160"
     CDS             492..719
                     /locus_tag="PWS53_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872364.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1355..1726
                     /locus_tag="PWS53_002161"
     CDS             1355..1726
                     /locus_tag="PWS53_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872365.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1982..2224)
                     /locus_tag="PWS53_002162"
     CDS             complement(1982..2224)
                     /locus_tag="PWS53_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0872366.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2256..3158)
                     /locus_tag="PWS53_002163"
     CDS             complement(2256..3158)
                     /locus_tag="PWS53_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0872367.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3171..3359)
                     /locus_tag="PWS53_002164"
     CDS             complement(3171..3359)
                     /locus_tag="PWS53_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0872368.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3373..3936)
                     /gene="amaP"
                     /locus_tag="PWS53_002165"
     CDS             complement(3373..3936)
                     /gene="amaP"
                     /locus_tag="PWS53_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0872369.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3974..4867)
                     /locus_tag="PWS53_002166"
     CDS             complement(3974..4867)
                     /locus_tag="PWS53_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0872370.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4945..5868)
                     /locus_tag="PWS53_002167"
     CDS             complement(4945..5868)
                     /locus_tag="PWS53_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0872371.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5917..6267)
                     /locus_tag="PWS53_002168"
     CDS             complement(5917..6267)
                     /locus_tag="PWS53_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0872372.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6300..7202)
                     /locus_tag="PWS53_002169"
     CDS             complement(6300..7202)
                     /locus_tag="PWS53_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0872373.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7195..8052)
                     /locus_tag="PWS53_002170"
     CDS             complement(7195..8052)
                     /locus_tag="PWS53_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0872374.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8386..9195
                     /locus_tag="PWS53_002171"
     CDS             8386..9195
                     /locus_tag="PWS53_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0872375.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9235..9732
                     /locus_tag="PWS53_002172"
     CDS             9235..9732
                     /locus_tag="PWS53_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0872376.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10117..10190)
                     /locus_tag="PWS53_002173"
     tRNA            complement(10117..10190)
                     /locus_tag="PWS53_002173"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10154..10156),aa:Ile,seq:cat)
     gene            10377..10700
                     /locus_tag="PWS53_002174"
     CDS             10377..10700
                     /locus_tag="PWS53_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872377.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10864..11118
                     /locus_tag="PWS53_002175"
     CDS             10864..11118
                     /locus_tag="PWS53_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872378.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11188..11433
                     /locus_tag="PWS53_002176"
     CDS             11188..11433
                     /locus_tag="PWS53_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872379.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11509..11874
                     /locus_tag="PWS53_002177"
     CDS             11509..11874
                     /locus_tag="PWS53_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872380.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3591 a         2327 c         2131 g         4421 t
ORIGIN      
        1 gggataaaaa tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc
       61 gtatttattt tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc
      121 aattttgatt ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg
      181 atcgttattg cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt
      241 tccactcaaa gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc
      301 gccttttttg gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt
      361 ttgatgttgg taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc
      421 gtgctggaaa aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg
      481 tgaggtgcga catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga
      541 gtgtgactga attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa
      601 atggaaaagc aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt
      661 gtcaaccagg tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa
      721 ataaagtaga acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta
      781 gaagttattt acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata
      841 aataagatat gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt
      901 tcctgataca actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt
      961 attcattcca ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg
     1021 ccattctgtt tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact
     1081 cttactccat tcttttttag taagctccca tatatactgt aaagccttct atatattatg
     1141 tcatgttgct ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat
     1201 ggacaattct tttttatttt taacattatt ttgataataa tgattattta tttaaaaata
     1261 cattagataa catttgttac gacacgaaaa agcttcacaa acaaatatcg ttattttaaa
     1321 atgagggcga actcacacaa ttaggaggaa tagtatgcca tttattgttt acttttttct
     1381 ttcattactc gtgatctata ttccattacc aacatttttc ctgctcgtca atcgtctagc
     1441 agagcaacat gatacgattg ctgctcttac gaaactttgt ttacttctat ttgtttgtct
     1501 agattacttt acgagctatt attggctaaa acagacaaga gtaaaaaaga gatattattt
     1561 cttccttgtc tttttcaatc tctttgagtt tctgatccat tgctttttac ttcatcagta
     1621 tcatacgatc catttgtgga tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt
     1681 cacctttcct atctcaagaa aatttaggga ggcattgttt tattaaagaa gcttctctgt
     1741 atcaataaag acacatttaa ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat
     1801 aaataagagg ctgtgacaaa agtcttgtca cagcctctta ttccgtataa acagtgttca
     1861 aaagcttgca tcttcgataa tatgcccaac caaatgaaaa atatgagaag ctatttttgt
     1921 ttggctgttc ttattacttg atgaagaaca gctttttcac gacctctttt taaaagtctt
     1981 tttacagttt tcgagcaaag aatgatacga ttagtactaa aatgatggca ccaatcaatg
     2041 atggcacgat tgccattcct gcaatacttg gtccccaaga acctaataat gcttgaccaa
     2101 gtgctgatcc aactaaacct gctacgacat ttgcaataat ccccatcgaa gaacctttat
     2161 tcgtgattgc tcctgcaatt gctccgataa ttcctccgac gataagtgac caaataaatc
     2221 ccatgtttcg ttcctcctaa tgttttactt ccttattact gaacccgtgg ttctgtattt
     2281 tcttttactt tttgtgcgcc gcttccagct gctgatttta ctttatctgt ttgattcgaa
     2341 gcaaattcac cggttttttt cgctgcgtca gttactttgt cttgcaccgt ttcttgatct
     2401 ttttcaaatt cttcttttgt tttgatgtcc actacgttag cgttgacttc gatcacttct
     2461 aaatgtgtca tattttttac ttctttgccg attacttctt gcatttcttc aaagattttt
     2521 tcgatatctt tgccatattc cacaatgaca tccatatcaa ctgctacttg ttttttaccg
     2581 acttctgttt cgatcccagc cgttacatta tctgtattca caagttttcc tgccacatta
     2641 gagaagaaac caccgtttgc agataataaa ccgtctactt gttcaattgc gattccgatg
     2701 attttttgca caactttatc ttcataattc agctcaccgc gcattgaatc ttcctgttca
     2761 gaatcatgct ttccttggtt atttgtatga tcagaaccaa gttttccttg tccgtttgtg
     2821 tggtcagaac taagtttttc ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc
     2881 acaggtttag aaggatcgat tgcatcatgt tgttctggaa ctttagattc tggagttcct
     2941 ggatgatgag gtgcatgctt ttctgcatca tgtttcttct cttctactcc ttgttgaacg
     3001 ttagggacta ctggatctgg atgtgcagat actggtgctg ttttgttctt ttcatcgttc
     3061 acaccatttg attggtttgg tgtcgttggg tctggatgtg cagacactgg tttttggttg
     3121 ttcattttat tgttattatt tttgttattg ttattcatgg atgtttcctc ctaagattta
     3181 aaatagtgat ctaagatttt cgtttgtttc agataaaaac cgatagcgac gccgatcgct
     3241 gccatgatca agatcaataa ggtcttgaaa aagcccacgg tgatccataa aacggctaat
     3301 atcaatccaa gtaccccaaa gatgataggg agcttgtatg tcgaaaatag ttctttcatt
     3361 tttggtcccc ccctattcta ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa
     3421 gcctgtgtat ttcaccgata ctttcactgg ctcttttgtt cccaatatgt gttgtacttc
     3481 attttcgatt tctttcatca atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa
     3541 ctcccctttt acattgacgg cgatcttgtt tttcgttgct ttgacattta cttttggtga
     3601 atggatgaat tcatcttcat gcaagtgaga acgaaccatt ccttcaattg cttttttatc
     3661 caacgtcaac tccccatcac catgtttcag tagaaagttt ccgattgttt ttggataaaa
     3721 caggaaaaca agtaacgtga taattgtcaa aatagcaaat gcaacggcaa gccaaaatac
     3781 atattgttgc acgtatgaat tagtgatgga aaagaatcga aacttctcta gctgaaaagg
     3841 caatggatag gttgcactta atagtatcgc aaacaaaagc acagatagca aaagtaatgt
     3901 tccgatcact ccgatcgttt ttacaccttt attcattttc gcacctcctc ataggggtga
     3961 aacgatcaac ttcttataag ttgatcgctt ctccaccagt tactccgtaa atttgggctg
     4021 taacataact tgctttattc gatgctaaga aaacataaac tggtgctaat tctcctggct
     4081 gacctgcacg ttctagtaaa ctttgctgac caaattcagg gatcgaatca gaaggttgtc
     4141 cttcgtctag ttgtaatggt gtccagattg gaccaggtgc tacaccattt acacggattc
     4201 ctttaggtgc aagctgtttt gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg
     4261 ctgcgtaatc taataaatgt tcgcttggat tgaatgcttg aacagaagtt gttgttacga
     4321 ttgctcctcc tgctgggata tgcggaacag cttctttcac taaagcaaac atagcgatga
     4381 tgtttatcat aaatgtgtct ttgacttgct caagtggtaa ctcagcaata gactgacaag
     4441 aaatttgctg tcctgcgttt agtaccatcg tatctagccc gccaagttca ctcacggctt
     4501 tatctatgat ttctttaggt gcttgttcgt cacgaagatc aaaaggtagt aagacaatct
     4561 tacgtcctgc atctttcacg tattttgcta cttcctctgc atctttttct tctcctggga
     4621 agaattgaag agcgatatct gccccttctc tagcaaatgc aatcgctgct gcacgaccga
     4681 tccctgaatc tccaccagtg attagtacac ggcggttttc tagttgatta ttgccttcat
     4741 aggattcttc tccacaatca ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct
     4801 gatcttgttt aggaaatttc tccgtatggt acaagtttcg cgggtcgatt aattttggtt
     4861 ctgtcattgc tgtattcctc ctagttttta aattgtattg ccaaaacaaa tttctttgtt
     4921 tgatacctgt tttaaataga ttctttaatt tgtataatct ttccggtata aatggtaacc
     4981 taaaactact gctgctatca aagagccaaa gactacgagt ataaagccta gagcagaact
     5041 tttacccggt aatcctcctg tattcattcc ccaaatccct gcgaacaacg ccggaacgga
     5101 aatgattaat gcagctgaat ctagatattt catgatatga tttagattat tgtccatcat
     5161 cccgttgaat agatcaccaa tactctctaa taaatctcga tagatctcga tcattttttc
     5221 tgtttgtcgc tgtctcaaat ggacatcata gagtagctgt tcatcgtcta aacgtttgat
     5281 gaaagtttca gaattccata attgatccat tgtttcccgt tgatctctta aggtatgatc
     5341 gatatatacg atgtctcttt caagatctgc ctgtttatac agttcttcat tttccgttgt
     5401 tttacgagct gctttatcca aagcgtcaat tcgctttttc atctctctta gttctttcac
     5461 gtaatgggtg taaatatcta ataatgtata cgtgatgatc ttttcaaacg agtttaaatg
     5521 atttccatgc tttgtcagca gccgatccac aaattcggtt tttctatcga tacaagtaat
     5581 caagagatta tcggaaagta caaaagatat tggcgttaat tttttttcga tacgattatc
     5641 tgactgatct agattaatca ctaccaaaga aaatggatgc tctagtttcg tctcataaag
     5701 gcgctccatc cgagaaactt cttccggata ttcggttcct acgaaaatat catcaggaag
     5761 ctcgaattct tcgattgctt gctcaatctc ttcgggcttt gcactatcaa gtacgagcca
     5821 attatatttt ttttcatctg tttctattac attcttttct tcatccacaa agtagtatct
     5881 caatgtatac ctcctttttg gactttctcg aaactattag ctgaagaagc agttctttaa
     5941 tttgatatta taatcgagta ctgcgatttc attttctaat gaaaagctgt caagctgttt
     6001 gttgaagtcc atgttcaatc gtttaccgaa ataattttgc tctaatttag atgtatagat
     6061 gtccaataat ggatgctcga ttttagtcgc atattctcca aattccgaaa caaaaggaat
     6121 gatttgaaaa aagctgcctt ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc
     6181 attgataagc gcgagtgcga tttttgtatt ttccccaacc ttttcaatta acgtctctac
     6241 tgcattttta gacattgtaa tttccattcc tactccccct cttcagttgt tgtcttctat
     6301 tatttaacaa ctacatctgt ttttggttga acttttgcat attttggata tttagggaat
     6361 ttagctacac aacggaaagc cgtcatccct gcatattttt cgtaatattt cttcgttggc
     6421 ccttcaaaat ccttgctcca cggtttcaca tgtcctgaaa aatggataat gtaaggattg
     6481 ttgcgagttt cttcgtattc acgttcacct gctgctgttg gatgtttttt tgcttttgcc
     6541 atgatatagc cttgtgcatt ccaacgcgga tgaagtggta accaacggtc atgtaagata
     6601 gcatttaatg catcttggtc atggaatcgt aatttttctg gattatgttc gatgaaatct
     6661 aatacttttt gcgtaatatt ttcatctaac cattttttca cattgatcag catcaaacct
     6721 gagttgaaat aacgcattga tttagctgaa attttcattt tttccagtcg ttgatggaac
     6781 ccagcgtctt caacagctgc tacgattgaa tcaccgaagt caagacgcca taatttagag
     6841 atatcttgta aagcgatcat gtcgcaatcc atatataaga tacgttcaac ttctgtgccg
     6901 cggaacaatt ccggaatcgc aatacgataa taagctgttt ctggaatacg atcgctgatt
     6961 accacatttg taaagaattc tttattgatt ttcaagaatt ccacatctga gttcattcgt
     7021 gcgtttttca ctgagaagcg taaaagctgt ttactttcaa aatcgatatc atcgtcaatc
     7081 acatagaacc gaacaaattt agacgggttg caattatcta atactgagac aaataatgca
     7141 gctaaatgtg gtacgaattt tgtgttacaa cttgcgacta ctgctatttc ttttttattc
     7201 attgatctcg cctactttcg ttagagtact gtgtgctaat ttttttaagt ataaattggt
     7261 atacggatga tctttcaacg tattccaagg tttatcatgt cctgtaaagt gaacgatcga
     7321 aggtttttca tttccttctt tatactgacg ttcatatttt tcatttggtg ctggatgacg
     7381 ttcaaaaatc agcgaagtct gcatgttcca ttttggatga agttgttccc aatcttcata
     7441 aagtacagca ttcaaagcat cttggtcgtg atagatgatt cgatcgccat tttcactgag
     7501 gtaatggata gttttttctg taatttcttt tttattccat tgatcaatgt caatcaccat
     7561 cactcctgaa ttgaagtaca agtcatcgga atcgattcct aatctttcca aagctttcac
     7621 ttggcctgga tcgataatag cgccgattgt ttttccattt aacggctcat catatagttt
     7681 tacgatatcg tctagtacta acacatctga atctaagtaa aggactttct tataatcctc
     7741 tttagctaat aagtttggta aagaaattct taagtaagcg gtagttgtga tatggtcact
     7801 gactaggaag tcttcgtaga tatctttttc cactgtcagg aattggatac ttgcattact
     7861 tgaatattta tttaccgttt cttccaatcc ttctttacta tattctgata gaccatcgtc
     7921 aatcacataa aattttatgc gtcttgtttt attgcaattt tctaatgcag ttgcgatcat
     7981 cacgctcaag taaggagcat aattttcgtc tgaagcagtt accacaggaa ctacgccgta
     8041 tctcttctcc atcaaaatca ctcctttttc taatcttttt ctaacctgtt ctacatctag
     8101 aatattcctc atttggtttt aaagcaaaat atatgctttg ttttcattga atattttaaa
     8161 atttttttat cacaaaacgc atcggtaagc gctaactatt cgtttttcat ggctttttat
     8221 tcaaaggtat tgaccaaaaa ctactatata tagccacctt tttcttaaga atgttttttg
     8281 aaaaaattca taatgacaaa gcaagttagt ttcatttttt taaattctca tttaatctta
     8341 aggtaacagg aaaatagata tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg
     8401 attgatttag acggtacctt gcttgatcga aatggaaaga taccagaaga aaatatccaa
     8461 gcacttcgga aatttgatga aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc
     8521 atttctgcta aagatatttt ttccgaatta ggaatcggtg gctatttgat ttcttccaat
     8581 ggttcactga ttgctgaaat gaaggatgga aaaatggcga aagttctcag acgttcaaaa
     8641 atagatagcg atacactgag aaaagccttc ttctatgctc aagaagcgaa aatatccatc
     8701 attgctagcc gagaaaccca agatgatcag ctgacattcg atgaatcaaa tctagtcaaa
     8761 gatgaccctt attatgaaca tttcaattta cagaaccatt cttttgatca aattttaaat
     8821 atgttggaag atccttctct tagctatctt aaattagcat taacagataa gagtgaagaa
     8881 aaactacaga agctacaaag agatctgcaa gcagatggta tccaatcagt attctctgat
     8941 ccgcatttct tagaaatcac gcctacaggt atcactaaag ctcattcact tgtttttctg
     9001 gcggattacc ttggaatcga atcgagtgac atcatggctt ttggcgatca agaaaacgac
     9061 attgctatgt tgaattacag cgggttgagt attgcgatgg ggaatgcaca gcgacaagta
     9121 aaagaaattg caaaccaagt aacactgacg aatgaagaag ctggtgttgc ttatatttta
     9181 aaaaacttct attaaaaaga ataaaatgac gatatgaatg taggaggaaa gaaaatgtta
     9241 gttattattg atatgcaaaa tcatattctt gatccaaaca gcgaatttta tattccagag
     9301 tcagatcaat tggcagaacg tattgctcaa agattagcta aagcacgcca aaataatgaa
     9361 tatattctct tcacacggga tataccagtt gaactgaagg acaaagaaga agaacgagaa
     9421 gatttgcaat tgatccctct tctttctcca caagaaaatg aacgagtgat taaaaagtat
     9481 tactttacga ttccacctga aactttagct gaaatccagg aaactttatt tgaaagcaaa
     9541 gaagaacaaa aagagatcga agtagtcggg attgaaacaa acttgtgtgt actctctaat
     9601 ctgataggcc tgcagagtgc ttttcctgaa gcagactttt ttgttgatcc aatgttagtc
     9661 tccagtagaa aacacggaga atccgcttta gagctgttga aagatttcaa cgtctctatc
     9721 gtcgaatcat aaaaatatct atcttcattt acgatattta acacacaata aaaaaagcac
     9781 gcagaactgt tttagtccta cgtgcttttt ctatcaaagt agattggctt ttttatgaca
     9841 cagagtgaaa cggcaggctc acgacctctt tcttaaatgg tgttcaatca gctgaccctc
     9901 ggtattagct taacaaacgg caaaatatga ggaactattt ttccgtttgt taagctaata
     9961 ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtattcaaa agcttgcatc
    10021 tgcagtaata agaacagcca agtaaaaaca taagaagctg tttttacttg gctgttctta
    10081 ttacttgatg cagggcagct ttttcacaac ctcttatgta ccttgtagga ctcgaaccta
    10141 cgaccggacg gttatgagcc gtctgctcta accaactgag ctaaaggtac atcataaata
    10201 aaatttattt ttgttacttt tcaactatag cacgaatgac aaactctcac aagtttcaaa
    10261 atataagtaa aataatattg ttattcttat ttcttttact tgtgtcataa ctattttgtt
    10321 actttaattt ttatttgctt attttagaaa aaatgttatt cttttattta ttctcaatgt
    10381 taggagtgat tattttgcca tttattgcga ttttattcga tttagttgcg gcagcagctt
    10441 attttctcca attaaattat caaacctcag gaattctgtt tattggaatg atttttcaag
    10501 gtgtgattac ccttttgctt cttgtcatga tgctcagcta taaaggaaaa agatatgccc
    10561 gtattcaaac aaaagtattt gttaaatatg tttccattcg ctatgggatc atcattcttt
    10621 cctttttagt gaatgcgatc gtcctctttt tatatgtatt gaattactta gatatcaatc
    10681 cattgatttt ttctcgatag ataagacaca ttcagcggaa cagttcctgc ttatcagcag
    10741 aaaactgttc tgctttttca tttttcattg atctattctt cttttatttt cctagcattt
    10801 catttgtgat gtatgcaata acatgttatc ttttattata tgaatcgttg aggagtccgt
    10861 tacatgatta cgttggtgag agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt
    10921 attgtttatc ttatgaattg gaacaaagaa cggttttatt tagctatttt gactcttccg
    10981 gctatctact ttatgtggaa agtttttaat tacaactact ttgaacccga tagtgttttt
    11041 atagaagaat tatcaggact ggttttgtct ttattgatcg tcattcttta tttgatacgt
    11101 ttaaataaaa aacattgagt actattgatt gcatgtgtag ctttctatag tcattataac
    11161 tattctacta taatgaaagg tgattaaatg agtatcgaag aagctatttt actggatact
    11221 ttacgttgtc gtctgaattt cttaagaact ggccgatctt tatctcaagt agctgtttct
    11281 agcgcgttaa gtattccttt attcaaatat cgtaagtacg aagaaggaga agaaattcca
    11341 agcatcattg aattaatcta tatcgctgac ttttacgata tttccgttga ttacctaatt
    11401 ggtcgctcag aaacagcaag caaactgaag tgattttttc cagccttggt gctggttttt
    11461 tcatttaacg tctaaggtta acaatctata tataaaggag acatttctat gcgattatgg
    11521 catgaaaaat tgatttcaaa gcttcctcgt caacagcttt taggacaaca tcgggaatgc
    11581 tgtgcattaa gaggaaatgg ctggggaaag aaacatgcta ctgtggatta tgtttttcac
    11641 tattctcctt atagattatt ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt
    11701 ggttacagcc cagcaccaga gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca
    11761 ttttttgatt tacctactgt agagcgcaac gtgccgattt atccggaaca cgatgaagtt
    11821 tatctagcag aatgtttagc aaatttggag caaaaaggaa tttatcttac ctaatataac
    11881 tagaaaagcc ttctatctcc tcttacttca ttaaaaagct ttaaccatct tagatcaagc
    11941 taaaatgtcg taagttttag ggttatcttc atcctcacgt gcaaaactaa aagcgtactc
    12001 tagttttggt tcatcgccaa cttccctaga cataaatgaa catctcctga cgtggtttaa
    12061 aagtaccgca tcttcgacgt ctgtatacga tgcacagcac agattgcttc ttttgatctt
    12121 tcttttgatt tttctgttgc ttcttctaca ccttcttcga ttactacatc gatgcttaca
    12181 attgcttctg cctaatattc ccatgcatcg tcgatttaag cctctgtagt cactgtgatc
    12241 gtttccacat tttaactcct ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc
    12301 taagccagag tcagaacaac ctgccattgc tagcgttaat attgatagta aaaatagttt
    12361 ttttatcata aggttccccc cctgaaaata gactaaaata ataacgcttt ctttttttgt
    12421 tttcagacga aaattaataa tttctaaata ttctttaaaa aagcttataa
//
LOCUS       DANJJH010000081        12344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000081 DANJJH010000000
VERSION     DANJJH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..2115)
                     /locus_tag="PWS53_002178"
     CDS             complement(40..2115)
                     /locus_tag="PWS53_002178"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL0872381.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="PWS53_002179"
     CDS             complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="PWS53_002179"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL0872382.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3418..4230)
                     /gene="recO"
                     /locus_tag="PWS53_002180"
     CDS             complement(3418..4230)
                     /gene="recO"
                     /locus_tag="PWS53_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL0872383.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4373..5272)
                     /gene="era"
                     /locus_tag="PWS53_002181"
     CDS             complement(4373..5272)
                     /gene="era"
                     /locus_tag="PWS53_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL0872384.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5287..5682)
                     /locus_tag="PWS53_002182"
     CDS             complement(5287..5682)
                     /locus_tag="PWS53_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL0872385.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="PWS53_002183"
     CDS             complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="PWS53_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL0872386.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6159..8351)
                     /locus_tag="PWS53_002184"
     CDS             complement(6159..8351)
                     /locus_tag="PWS53_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL0872387.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8375..9346)
                     /locus_tag="PWS53_002185"
     CDS             complement(8375..9346)
                     /locus_tag="PWS53_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL0872388.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10052..11431
                     /gene="brnQ"
                     /locus_tag="PWS53_002186"
     CDS             10052..11431
                     /gene="brnQ"
                     /locus_tag="PWS53_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0872389.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11585..12031)
                     /locus_tag="PWS53_002187"
     CDS             complement(11585..12031)
                     /locus_tag="PWS53_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL0872390.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12058..12234)
                     /locus_tag="PWS53_002188"
     CDS             complement(12058..12234)
                     /locus_tag="PWS53_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL0872391.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT         3317 a         2616 c         2236 g         4175 t
ORIGIN      
        1 attttgtgcc tattctctgg tttatttaca tattagaaat tattttgtga tgatttgatc
       61 taaacttgct agcgacaaag ccatggcagc aatttgactt aattgcgtta atcggttctt
      121 acgtactgct ggttcttctg ccatgaccat cgtctgatca aaataatttt cgatcagtgg
      181 gcgaaggctt acaagctgat cataattttc gttgatcgtt tggtcagcaa agcttttttt
      241 cattgcctct actgctttgt gcagttcttt ctccgcttca ttttcgaaca gttcagggtc
      301 tacttctgat tgtttttcaa gctccgcttt tttcgctaag ttcacgacac gtgtcaaagc
      361 ttccatcgat ggtttgaagt cttcatctgc aagatgctgc ttgaacaatt gtgctgctgc
      421 aaatactttt gtcagatctt tttgttctgc attcaaaaca gcttcgatga catcatgacg
      481 aatattcttc gttgtcaata attggcgcag acggcctttg ataaagtcga tcacctgttg
      541 ttgcccttct gataaaccaa tgccgaaacg atccgtatct ttactgataa catcactaat
      601 cgtttcttgc agaacagaca aaggaaaggc ccattttttc gattcgatga tccgaacgac
      661 accgtatgct tggcgacgta aggcataagg atcgtttgac ccgcttggaa tcatcccaac
      721 tgcaaagaaa gaaaaaatgc tatctaattt atctgcaatc gctaaaacag aacctactgt
      781 agactgaggt agctctcctt cactggatat aggcatgtaa tgctcacgaa tcgctgttgc
      841 tacagcaggt ttctcgcctt tcagcagagc atatttttcg cccatgatcc cttgtagttc
      901 aggaaattcc cctaccatgt tggttactag atcaaattta tagatttcag cagctcgttg
      961 cagatccatc aattcttcct cagatagtcc tacttgttcg ccaatgatac gggcaatgac
     1021 ttgtacccgc tgcattttct catagatact accgattttt tcatggaaag taacagtttt
     1081 tagtttttca acgtattggt cgattgaagc tttcttgtct tcattataaa agaattccgc
     1141 atcttctaaa cgagccgtca atactttttg attgcctcga accacatttt ccaattttac
     1201 ccgatcacca ttacgagcag aaataaaatg aggaagcagc ataccttgat cgttccgtac
     1261 ctcgaaataa cgttgatgtt ctttcataga agtgactagt acttcttcag gaacagaaag
     1321 atatttttct tcgaagtcgc cagaaaagac agttggatat tcgactaaat tgttgacttc
     1381 ttctagtaaa tcttcgtcta gatcaataac ccagtgattt ttttcggcta attcatttgc
     1441 ttgtgtaacg atcatttctt gtcttttttg cggtgaagca ataacgaatt gttcattcaa
     1501 tttttcttca tattcttttg catgcgcaaa tgtcacatct tcgcctaaga aacgatgtcc
     1561 tcgagaggct ctgcctgttt tgacatctaa gatctcaaac gggattactt cgtcatctaa
     1621 caatgccacg atccagtgaa tagggcggat atattcaaaa tcgtattctg cccaatgcat
     1681 cgttactggg aaagtgaggc ttttaatgac atctgctaaa cctgtcaata cttctttcgc
     1741 ttctttgccg tgagtgtatt tcgttacata gacatattcc acgcctttta attctctaaa
     1801 agtgatagca tctgtcgtca gaccttgtcc gcggacaaat ccttcagctg ctttcgtcca
     1861 gtttccatct gcgtccaaag caattttttt ggctggtcct ttgacttctt cttcactgtc
     1921 tgcttgacgt tctggaatgt ttgttacttt gattgctaag cggcgcggtg tagaaaacgt
     1981 ttcgatcgta tcataagtca attggttttc atctaaaaat tttgctgttt tttcttctaa
     2041 ttgtttcatg cttggtgtga caacatgagc aggcatttct tccagcccga tttcaagtaa
     2101 taagtctttt gccatcttat tcagcctcct tcatgttttc tggatttgct gtgtttttat
     2161 tcaataaagg gaatcctaat ttttcgcgtt ctgccacaaa gattttggct acagcacgag
     2221 ccatgttgcg gatacgggaa agataacctg ctcgttctgt tacggatact gcaccgcgag
     2281 catctaataa attgaatgta tggctgcatt tcaaaatata atcatacgct ggatgaacaa
     2341 gattttcatc gatacagcgt ttagcttctt tttcaaaacg gtcaaagttg cttagtagca
     2401 agtcttggtc acttacttca aaagaatatt tggaatgttc atattctggt tgtttgaaga
     2461 tctcgccata tttcacacca tcagcccatt ccagatcata gacactttca acttcttgaa
     2521 tatatgaagc aagacgttct agtccataag tgatctcaga agtaactggt ttacaaggta
     2581 gtcctccgac ttgctggaag tacgtgaatt gtgtgatctc catcccatca agccatactt
     2641 cccaacccac accggcacac cccatcgatg ggttctccca gttgtcttca acaaaacgaa
     2701 tatcgtgttc taatggatca attcctaaaa gacgcaagct ttctaaataa agctcttgga
     2761 tattttcagg tgaaggtttc attacgactt gaaattgatg atgctgatac aaacggtttg
     2821 ggttttctcc ataacgcccg tcagctggtc ggcgggatgg ctctacataa gcagcattcc
     2881 atggttccgg tccgattgcc cgtaagaatg tatatgggct catcgtacct gctccttttt
     2941 ctgtgtcata agcttgcatc agcatgcagc cgtttgatga ccaaaatttt tgtaatgtta
     3001 aaatcatatc ttggacggtc aattttttac tcatctgttt tgcccctttc aatttttaag
     3061 cgttgctgtc aataagaata caggaaagaa aacgacaaaa agtccctatg tctgattaca
     3121 gacataggga cgaataattt cgcggttcca ccctacttcc gaataaagat tcggcagctt
     3181 catttatcgt actcgcaagt gcctcttcaa aaatcgtttg ggtccggctt ccactctccc
     3241 gaactcgcta aaccacgtgt tttttactga tcttggtcaa tgtacgtatt cattttgcag
     3301 ttttcttgtt tatataatag atcctctcag tgaacgatct atttaatcta cggttaataa
     3361 tagacggaag tccctgtatt tgtcaaatag ttttgatttt tttgttgtat ctttcattta
     3421 ttcttctttt tcaggtatct ctttttcagg catatttttc tctggcgtct ttaatacgtg
     3481 tgcccaagac ttcatttgat caatgaactt tttgcttttt agatgaatac cgacatattc
     3541 atcatacaac tggtcaatga ggtcacggat tgcctgcttc gtttcttctt tcaagtggat
     3601 tccactgatc ttatcataag aaatagccga aaacattcga acgaaataaa cagcgcgagg
     3661 atctgcatga taacggtgtt cgtccattgg ccagtgtttc tcgcatatca caccgccaga
     3721 tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacacagt gcgtccaaat
     3781 tggcgcgatt ccgaagcgtt gcagtatttg aacttcaaag atattcgtga tgatttctgc
     3841 atcatctcct tgatccatcc gctgcagagc ttgttgcgta aattggaata aatgcgggtc
     3901 atattgatga tcttcgattg ctgcatctac tagatttagc aaataggtac cataaccgct
     3961 caaaaagata tcttcttgga tgttccgaaa cgcatggacg tctttgacac tgcttaaaaa
     4021 cgacaaacct tcacttcgaa aattaccgat atagactgct tcggtaaagg ggagcaaagc
     4081 cggagctaat ggctgatttt gccgatggat tcctttgata taaaacatca ttttgccaga
     4141 agattccgta aagattttga ctaatttatc tttttctttg taattacggc tagataaaat
     4201 caagcctttc gattctgcaa gctggctcat attctccctc ctaaaaaata aatctggaaa
     4261 agcagattct gccgatccag attatttatg tttcgatctt ccgccgtttt tctcaacgtg
     4321 cggattttta tccagtttat ttctatactt aacctataag aagttcagaa tatcagtatt
     4381 cgtcttttcg gtagccaaag tcttgcagat agactttttt gtctcgccag tctttttgga
     4441 ctttgaccca aagttctaga aatactttgt ctcctaaaag attttcgata tcctttcttg
     4501 ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatt cctttttggc
     4561 tatctctttc gacgatgatc gtggcctgga tatggatttt atcattctcg tttcgcttca
     4621 tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa aacaagactt
     4681 tctcacggat caattcagaa acgataaagt attcaggatg atcagtcact tgatcatctg
     4741 gaaaatattg tgggccctct ggcatttcac tgactaatac atccattaag cgttcgacat
     4801 tgtttccttc tgtagcagag atcggaatca cttgtgcaaa aggcatctgc gtcgaataat
     4861 cttcgatgat tcccagcaac tcgtctggat ggatcttatc gattttattg ataatcagat
     4921 aaactggaga atgcacgttt tttagacgtt caataataaa atcgtctccc cggccgcgtt
     4981 tttgatcagc actgatcata aataagactg catccacttc tttcaacgca ctgtaagctg
     5041 cttctaccat aaaatctcct agacgatgct ttggtttatg gattcctggt gtatcgataa
     5101 aaatcagctg agcttctggt gtagtatata ctccttggat tttatttcta gttgtctgtg
     5161 ccttgtcact cataatggca attttttgac ccacgattcg gttcaataat gtggattttc
     5221 ctacattcgg gcggccgacg attgcgacaa accctgattt atgttctggc atatacgttc
     5281 tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca acccaatgat
     5341 gacagcaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga tttttttccc
     5401 aatcggatga aaatgaaaat ccgtaaacat gtccactaca ttttcaaaaa cagtattgat
     5461 gatctctact atccaaacga gaaaaacagc taaaagcagc cataaccact ctatccgatc
     5521 aagctggaaa atcacgccca taaataaagc aaggataccg aacgcaacat gtttcctcat
     5581 attgcgttct tcctcaaaaa cggttttgac tccttggaca gcaaattcca cagacatcag
     5641 gaaatgtttg tttttctcta cgtttttatc ttttgagtcc ataggcatcc aagatctctt
     5701 tctgtaaacc aaacatttct ttctcatctt ctggtgtcat atggtcatac ccgttgatat
     5761 gcaagaatcc atgtaccgct aaaaagccaa gttcccgatc atatgaatgg ccgtaagctt
     5821 ctgcttgttc agcagcacgt tcaatagaaa tcatgatgtc tcctagtaca cgaggcaatg
     5881 gattctctcc atcttcaaaa atcactggga tttcgtcttc gccttcttct tccagagcaa
     5941 aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt acttggattg
     6001 ccgcattgtc catgaaagta acagacattt ctgtttcctc tggcagcttt aaataagatg
     6061 ctgcaaattg cagcaagtca tcgatttctt tcattttctc ttctgagact tggtttgttt
     6121 catccataaa ggtaatatcc attatacatc gcttctttct attcttcttg ttcttccttt
     6181 atcttctcaa cttctgtttt cattttagga tatttgatcc gagaatggaa ggtgctactt
     6241 aaaccgctga tcaatgattt ttccactttt cggatttctt tcaaggtcag tccactatcg
     6301 tctaattgac catcggaaat acgctcttcg ataagattat ggacaaaact ttcgattttt
     6361 tcgctcgtcg gatgatccat tgcccgtact gcagcctcac aactgtcagc aatattgacg
     6421 atccctgctt cacgactttg cggttttggt ccaggataac ggaattgctc ttctgttact
     6481 tccggattgc gttctttcgc tttgatgtag aaataacgca ttagcgtggt tccatgatgc
     6541 tgtttgcaaa tatcgatcac catttgcggc atattataat cttccaatat tttggctcca
     6601 tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa attgtgcggg
     6661 ttttccgctc ctgaaggcaa gttctcaacg aaaaagctag catgtttgat cttgccgata
     6721 tcatgataat agcaagctac tcgagttagc aaagaacgtc cgccgatttc tgctacagcg
     6781 ttagcactta gattagctac catcatacta tgatgataag tacctggcgc ttcttccagc
     6841 aactgtttca ataaaggatg atttggattg ctgagttcat ttaatacaat tacactgtca
     6901 tcattgacca tcaattcaat atatgggtgt agaccgatcg tcagcaagaa tgagaagagt
     6961 gcaccagtga acccgcagac aagcattaac caagtcgttg catcaccaaa gctcattcct
     7021 tgatagacga tcaaaatgac atccatcatc acaggaaaaa taatgaccca catggcagca
     7081 gataatcctt gttctgagat ccgtttgcgt ctgacaatcg ttgccatgag cccagagaaa
     7141 acataagaca tcaagataat cgttagaaag ttcgtgccaa tcgaatcata aaaaatgaaa
     7201 agtgcagata ctgcttggaa aataccagcc atgatccctg ctcttcggtt caagaaaaag
     7261 cttaaaacaa gtggtgcaaa agcagcggga taaaacagcg ggatataagt agcttgttcc
     7321 gtttggaata attggaaaaa tttcatcagc actatgctga ctgacatagc tgtcacatag
     7381 aaaagcacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt ataaatcagt
     7441 acgccgattt gaagcaaaac agctaaaacc atggcaatta gcggaaaaat cgaagttgtt
     7501 tgattagtca gtcctagtaa ttccaatttt ttcatagctg aagcatcgat ttgattacct
     7561 tcacgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc tcgtgcggat
     7621 tgtttcaatt cttctgtctt tttctcattc aatacgtcat tcactacgat accttgatcc
     7681 actaagtagc gaatcatttg ttgttgtgct ggtgtgacat tcaaatattg gatttgctct
     7741 tctgcttctt gtttaaaagc ttccaattca ctttcacgaa cttgtttgct catctgttcg
     7801 tcaatgagct tcagactctc atccctcaca gtatggatct cagtttcagt catttcaaat
     7861 atcgtcgaat aaaaattgtt cggtagtttt tgataaaacg caacattttc tgcattcaca
     7921 ttttcaaaat ttttcttcaa cgcggcgatc cgctcttctg tagtaggctt tgggatattt
     7981 tcatcttcct ttgccttatc aatcttttcg tcataagatt tgttgatggc atcattcgtt
     8041 ttatcgatca attcaaatag ttgcttgatc cgattgttct ggtcatctgc aagatctttt
     8101 tggtaagtgt attctggagt gactgcttct gctgccagtt ttcttttttg ttcggtttct
     8161 tccgtgttct cgatcgtttt gttcgcacgg atactttctt ctgctacttg gccttctctg
     8221 aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcagataat gataaaaaac
     8281 aagaacagca ggaaaggcaa tgctgccttt ccaagttttt cttgtagttt ctgcagtgtt
     8341 ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatggaga tctgcctttt
     8401 cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt gcttcaaaat
     8461 taacaaaatc gattttcttg atttgcttta atgttcgttc tgcatgaaca agaccacttg
     8521 ttgtccctcg aggcagatca atctgactcg tatctccatt gacaatcatt tttgattgat
     8581 aaccaagacg tgtcaggaac attttcatct gagcaactgt cgtgttctgt gcttcatcca
     8641 aaataacgaa tgcatcatcc aatgtccgcc cccgcatgta agctaaagga gcaatttcga
     8701 tcacgcctcg ttccatcaat cggttcgtat gatctaatcc aaaaatttgg tataaagcat
     8761 cataaacagg acgcagataa ggatctactt tttcttttag atccccggga agaaagccta
     8821 gattttcccc tgcttccaca gcaggccgcg tcaaaatgat tttttgcact tcaccttttt
     8881 tcaaggcagc gattgctaat actactgcaa gaaaagtttt tcctgtcccg gctggtccta
     8941 cgccaaatac gatatcatgt ttggctaccg attcgacata tttcttttgg ccgctgtttt
     9001 ttacccgtat tggttttcca ttacggtctt tgacgatctc ttcctcatac atttcaacaa
     9061 aataatctaa tgtgcctttt tgccccattt tcagtgccgt cacgacatct ggtgtactta
     9121 catggattcc tcgtttgatc aattcctgca atgtccgaat gactgaggca gccaaggaaa
     9181 tctgggattg ttcgccaatg atctgaatga tttctccacg tgtatggatg gttgtttctg
     9241 ttgtttcttc aatcagtttt atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat
     9301 cagcagccgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat agacatcccc
     9361 tttttttcga ttcattcttt cctattttac catactcaga agaaactata tagcaaataa
     9421 gaaaaggttc taaaagagtt gcttaaccat aagtaccaag aaacgttaaa aaatagctgc
     9481 ttccattctc taaaggtctc gtttgaataa aaaaagctga tcatgtaagc tatgcataag
     9541 atagaggaca agtattccgt ctaacttagc aagtattctc tgggtatttt tcttgtttca
     9601 aaaaaagaaa acaaaatatt tgaaatattc ttgcatttca aaaaaaacag gtgtattata
     9661 acgtcaacaa ataaatatac agcaaagcgt cgaggaaaac agtaggctat tcagcgattt
     9721 attcccagtg agtcatgtaa gtgagaaatg acaataaatc gaatagacga acatcatttc
     9781 cgagtcaatg tcgaaaacgt agatcattgt ggctgcctcc attatcaggc tagcggataa
     9841 ctttatcgaa aagcgtccgt gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt
     9901 tctagaatct agagcccttt tcccaagtgg aaaagggctt ttttttatat tttaaggtgg
     9961 tggttttcat ggaatctgat attagtggca tttctagttt ctgcaacgtt tattttgtta
    10021 ctcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt attcatggaa
    10081 gcaattattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag gtaatctgat
    10141 ctttcctgtc cacttaggtc aaatggctgg atcaaactgg atcagcgctg gtgtaggatt
    10201 tgccatctca ggcgctcttt tcccattatt agctatctta gctgtagtag tgacaaaaag
    10261 tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct tgttcttgat
    10321 ccttgttcat ttaacgatcg gaccattctt tggtacaccg cggacagcag caactgcttt
    10381 tgaaatggct gctaaaccct ttttgcctga aaaatatgat tcattaggta tgtttttatt
    10441 ctctgctctt ttctttttac ttgcttattt ccttactgta catccaacac gtttggtcaa
    10501 atatgtcgga aagtatttaa actccttgtt tcttgcatta ttagctatca tttttgtcgt
    10561 ttctttattc caacccatgg ggaatctaaa ccaatctgct gcagattctt atcagacgaa
    10621 cgctttgaca aatggcgttt tagaaggata caacacggtt gatgcagtag ctttacttgc
    10681 tttaagtgtc acttttgtcc atgcagttcg tgatcttggt tttaaagata aaaaagtcac
    10741 agaattaaca gcaaaagctg gtagcctgag cgttgcgtta gaagtcatga tttattttgg
    10801 tctggtcctt ctcggtgcga tgagtttgaa taagatgcct ttatcagaaa atggcggaac
    10861 tgcactttct cagatcacta atttttattt aggtaacttc ggtgctagct tcttaggggt
    10921 aatggtcaca ttaggtgtct tcactaccgc tatgggactc gttgtttctt ttgcacaaga
    10981 tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa cgacttttgt
    11041 ctcttttgtc gttgcgaatg ccggattgga taatatcatc caatggtctt taccagtatt
    11101 gatgcttttg tatccattgt cacttgcgtt aatcttgctt tccttaacag caaaattctt
    11161 ccaaaagagt ccatttgtct atcaagtaac gatgttcttt tctgccgttc cagccgtatt
    11221 ggatatgttg gcaaattcgc ctgcattagt gagtcagcaa actgtcgtag caagcttact
    11281 aggattttat catcatcacg ttccttttgc tgcattagga cttggctgga tggtcccaac
    11341 gttacttggt tacgctggca gcttactctt ttattacgcc tatcgcctat cagggtataa
    11401 acaagaagca aatgaactac cagaagaata accaaatcaa acacaattta acataagaga
    11461 tattaaacag gaaaaaaaca gctagaagaa agaatcttat tctttcttct agctgttttt
    11521 tagttctttt tttacgaaca caaaaaactg cggacaatca ctgtccgcag tttaaatgat
    11581 aaaattaaga ttgtaatagt tctttcacaa tcgcattcac ttggtttcca tctgcttttc
    11641 ctttgacttt aggcataatt gcacccataa ctttgccaaa ttctttcatt gatgaagcac
    11701 cagtttgagt gattgcttct tgaacaagtt gtcgaatttc ttcgtcggaa agttgttccg
    11761 gcatatattt ttcgactatt acaatttcac tttttacttt ttcagctaaa tcgtcacgtc
    11821 ctgcttcttc aaactcatgc aaagaatcac gtcgctgttt catttcgcga gacaacaccg
    11881 ttagttcttc ttcttctgtc aagtcgtgac cttctttaat ctgctcgttt tgtatagatg
    11941 acttgatcat tcggatgact tgtaaagatt ccttatcttt cgctttcatc gctgctttca
    12001 tgtcatcgtt taatctactt agtagtgaca cactaccaac tccaattcat cacagaacta
    12061 gaattttttg cgttttctag ctgcttcaga ttttttcttg cgtcttacac ttggtttttc
    12121 gtagaattca cgtttacgag attcttgtaa agtacccgct tttgacacgg aacgtttgaa
    12181 gcgacgaaga gcatcgtcaa gtgattcgtt tttgcgaacg actgtttttg acatgttaat
    12241 tccctccctc cgagcttaaa aagtaaccgt aattgttatt gaaatatatc tcaaaaaaca
    12301 agactgtcct gtaaatatta tatctaatgc tgttttgacc gtca
//
LOCUS       DANJJH010000082        12169 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000082 DANJJH010000000
VERSION     DANJJH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(72..629)
                     /gene="frr"
                     /locus_tag="PWS53_002189"
     CDS             complement(72..629)
                     /gene="frr"
                     /locus_tag="PWS53_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL0872392.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(632..1354)
                     /locus_tag="PWS53_002190"
     CDS             complement(632..1354)
                     /locus_tag="PWS53_002190"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL0872393.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1490..2371)
                     /locus_tag="PWS53_002191"
     CDS             complement(1490..2371)
                     /locus_tag="PWS53_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL0872394.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2470..3252)
                     /gene="rpsB"
                     /locus_tag="PWS53_002192"
     CDS             complement(2470..3252)
                     /gene="rpsB"
                     /locus_tag="PWS53_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL0872395.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3611..4090
                     /locus_tag="PWS53_002193"
     CDS             3611..4090
                     /locus_tag="PWS53_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL0872396.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4307..5398
                     /gene="phnW"
                     /locus_tag="PWS53_002194"
     CDS             4307..5398
                     /gene="phnW"
                     /locus_tag="PWS53_002194"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL0872397.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5391..5519
                     /locus_tag="PWS53_002195"
     CDS             5391..5519
                     /locus_tag="PWS53_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0872398.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5523..6068
                     /locus_tag="PWS53_002196"
     CDS             5523..6068
                     /locus_tag="PWS53_002196"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0872399.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6522..8213)
                     /locus_tag="PWS53_002197"
     CDS             complement(6522..8213)
                     /locus_tag="PWS53_002197"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL0872400.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8633..9580)
                     /gene="arcC"
                     /locus_tag="PWS53_002198"
     CDS             complement(8633..9580)
                     /gene="arcC"
                     /locus_tag="PWS53_002198"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0872401.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9695..10714)
                     /gene="argF"
                     /locus_tag="PWS53_002199"
     CDS             complement(9695..10714)
                     /gene="argF"
                     /locus_tag="PWS53_002199"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0872402.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10805..12034)
                     /gene="arcA"
                     /locus_tag="PWS53_002200"
     CDS             complement(10805..12034)
                     /gene="arcA"
                     /locus_tag="PWS53_002200"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL0872403.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3333 a         2526 c         2201 g         4109 t
ORIGIN      
        1 ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc ccctctgttt
       61 ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat cttatctact
      121 tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg caaatcatct
      181 tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc acgacggatg
      241 ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc taattctttt
      301 ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc attggttgga
      361 ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat gctcttgtca
      421 aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat ttggttcaat
      481 ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc gttcgcacgg
      541 cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt cattttttct
      601 tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt gttccgatat
      661 tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag acaagcagtg
      721 gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt aatcctttgg
      781 caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct actttcggat
      841 cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct gcgccgattt
      901 ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg gtaccgcctg
      961 caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga atatatggct
     1021 ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc acgttttcca
     1081 acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg taatcagctt
     1141 gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt ccgccgccca
     1201 caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg atgatttctt
     1261 tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct ccactcaatt
     1321 ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata ttttctattg
     1381 ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt gttttgctta
     1441 aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat tattttttca
     1501 cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct tcgccaactt
     1561 cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt tcaacagtca
     1621 tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg aatttcttca
     1681 agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc aatgcttgtt
     1741 ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat tcgtttacat
     1801 aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt tcgtcagttg
     1861 ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat ccaccaaatg
     1921 cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt tctccgataa
     1981 cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg attttcattg
     2041 cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct tttactaaat
     2101 cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca acgattgctg
     2161 cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg tcatttttct
     2221 tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc atgtcgcctt
     2281 ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt tcgcgtagtt
     2341 cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg tttttctttt
     2401 taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa acagctccat
     2461 cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga tttcttcgat
     2521 tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt caccttggtt
     2581 tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac gaattgcatc
     2641 gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat caaccatagc
     2701 tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt tacgtgggtc
     2761 aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta agaatttttc
     2821 aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa cttcaaaagt
     2881 tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt ggatagtatc
     2941 ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc ctgcacgagt
     3001 tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata atgctacgcc
     3061 gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt taactgtttt
     3121 ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct tcatttttgg
     3181 attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt gtttcataga
     3241 aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct tatcttctac
     3301 cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg gatttgctga
     3361 tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag agcaaatata
     3421 cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa gaaaaacagt
     3481 agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg tataaaagac
     3541 gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc tttcgtaaag
     3601 gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca ttgaagaatt
     3661 cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg ttaccgccac
     3721 gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac ctgatgaaaa
     3781 tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta ggactgagga
     3841 agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac gagtccactt
     3901 tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg ttttagatga
     3961 gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca tggtcatctc
     4021 ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa ctgaagtatc
     4081 acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta ttttcaatat
     4141 tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt tattcaaatt
     4201 tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta acaagagcag
     4261 cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt ataaattatt
     4321 aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga tcgatcattg
     4381 tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat tattggatct
     4441 tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca gctccttcgg
     4501 ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga tttgttcaaa
     4561 cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga actataccgt
     4621 ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc tgattgaaga
     4681 gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg gtattttgaa
     4741 taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta tagtggacgc
     4801 gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg attttttgat
     4861 ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac ttgctaataa
     4921 agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc tatatgacca
     4981 gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta cccatacggt
     5041 tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg aaactcgata
     5101 ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga tggggttcca
     5161 gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc ctaaggactg
     5221 gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg tgatctatcc
     5281 cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga tctatccgga
     5341 agatattttg gaactgacag caattatcaa agactataag gagaacgtta atgattaaaa
     5401 ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct cctgtccacg
     5461 cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa atccgctaac
     5521 cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct tctgtcaaaa
     5581 aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag gaattgtatc
     5641 aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg aaacccgaca
     5701 cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc acgaatggat
     5761 ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg tattcaccag
     5821 acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct tatatgatcc
     5881 ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa gtcggagata
     5941 cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt gtaattgaag
     6001 gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg gggaaaaaga
     6061 tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata tttgtgtaaa
     6121 caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag aatgactttt
     6181 atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa cagccaagca
     6241 aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
     6301 acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac agtcccctta
     6361 tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc attcagctga
     6421 ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct tttgtttctc
     6481 taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt atctggtgca
     6541 tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata aactagagct
     6601 aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata gtatttgttg
     6661 aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta tttttcccca
     6721 gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca gctggtatcg
     6781 tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc caacaggctg
     6841 actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa tcgaacaact
     6901 tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg gaaaatgact
     6961 gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg gtttttctcg
     7021 ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa taagacgatc
     7081 tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa tggaatatgg
     7141 tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa ctgtttaaaa
     7201 tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta agtacgttta
     7261 cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc tgacttctta
     7321 atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc tttatcctcg
     7381 ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc attgtagaaa
     7441 gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt attgaattct
     7501 ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc gccttcttcc
     7561 agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc agctacttca
     7621 tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac agcttcttct
     7681 gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca gtcacctaaa
     7741 tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat cgaattaccg
     7801 ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg agaagacatg
     7861 tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc tttttctttc
     7921 actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa gttcaagtaa
     7981 ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc taaatctgca
     8041 gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg aaatgcaaca
     8101 tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc cagtgttaaa
     8161 tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt catctttatt
     8221 cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg gcatacttag
     8281 tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct aaaaaaggac
     8341 cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc caagtgcgct
     8401 tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca agaatgatac
     8461 aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt atatcagaaa
     8521 aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa aggagaagaa
     8581 ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca aattaatcag
     8641 ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct aaagaagtga
     8701 tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct actttcggaa
     8761 gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct gaaacagtaa
     8821 cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg tctactcctg
     8881 tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa gcaaaatctt
     8941 tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca ggaatcccac
     9001 cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg acacgtgctt
     9061 ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt ccagcatctt
     9121 ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct tccgtgtaga
     9181 atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc acaataactt
     9241 gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact tcgcccatgg
     9301 cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta tctaaaggca
     9361 ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta ccgacttgcg
     9421 gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc actaaatact
     9481 ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta gacaagatgg
     9541 cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc ctttcttcag
     9601 ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc cctcaatgct
     9661 gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata aacaagttac
     9721 ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct tggtcaaatt
     9781 gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg atgccaaagc
     9841 gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc aagcagtgca
     9901 agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg atttggtaag
     9961 gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat acccatacat
    10021 cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta atcatcaatt
    10081 gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct tcaggttgta
    10141 attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg actaataaag
    10201 agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct tctactgttc
    10261 caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc cattcatcag
    10321 tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc attttttgac
    10381 tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct gtatcttctg
    10441 tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga tgtgcaccta
    10501 gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt tcaaataaaa
    10561 gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc aagtctttca
    10621 agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct ttttctgcta
    10681 ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt tagattgttt
    10741 ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc cgtcaggaaa
    10801 caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga ccaccacgac
    10861 ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta cgaagcaatt
    10921 cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt gccaatgtgt
    10981 ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta ccagttggga
    11041 tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag ttatctgaat
    11101 gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc ttaccgtctt
    11161 tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta aatacggtat
    11221 ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt tcaaatcctg
    11281 aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta gttcgttgag
    11341 aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct tcgatatgat
    11401 ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt ggatgatgtt
    11461 tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc atcttattga
    11521 ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag tttggcattg
    11581 ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg ctagaacgta
    11641 cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct tgcgtttcca
    11701 ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata tgagattcat
    11761 ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct tctgccgcta
    11821 atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca aagttgtcat
    11881 gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga tccataatat
    11941 ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc tttaatttcc
    12001 ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt ctttttgatt
    12061 acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca ttcaaaatga
    12121 gacattcctc acattaatat tcgataatta aataattaaa cgtttttct
//
LOCUS       DANJJH010000083        11808 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000083 DANJJH010000000
VERSION     DANJJH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(37..216)
                     /locus_tag="PWS53_002201"
     CDS             complement(37..216)
                     /locus_tag="PWS53_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872404.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="PWS53_002202"
     CDS             complement(597..1304)
                     /locus_tag="PWS53_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872405.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="PWS53_002203"
     CDS             complement(1297..1734)
                     /locus_tag="PWS53_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872406.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="PWS53_002204"
     CDS             complement(1749..2000)
                     /locus_tag="PWS53_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872407.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="PWS53_002205"
     CDS             complement(2000..2206)
                     /locus_tag="PWS53_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL0872408.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="PWS53_002206"
     CDS             complement(2643..2903)
                     /locus_tag="PWS53_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872409.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="PWS53_002207"
     CDS             2978..3181
                     /locus_tag="PWS53_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDL0872410.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWS53_002208"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="PWS53_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0872411.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="PWS53_002209"
     CDS             complement(3810..4709)
                     /locus_tag="PWS53_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL0872412.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="PWS53_002210"
     CDS             complement(4722..5315)
                     /locus_tag="PWS53_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL0872413.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="PWS53_002211"
     CDS             complement(5391..5540)
                     /locus_tag="PWS53_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872414.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="PWS53_002212"
     CDS             complement(5556..7760)
                     /locus_tag="PWS53_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL0872415.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="PWS53_002213"
     CDS             complement(7771..9231)
                     /locus_tag="PWS53_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL0872416.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="PWS53_002214"
     CDS             complement(9292..11664)
                     /locus_tag="PWS53_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872417.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2935 a         2451 c         1978 g         4444 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatgga
//
LOCUS       DANJJH010000084        11539 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000084 DANJJH010000000
VERSION     DANJJH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            6..305
                     /locus_tag="PWS53_002215"
     CDS             6..305
                     /locus_tag="PWS53_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872418.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWS53_002216"
     CDS             378..1739
                     /locus_tag="PWS53_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL0872419.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWS53_002217"
     CDS             1761..2021
                     /locus_tag="PWS53_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872420.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWS53_002218"
     CDS             2035..2787
                     /locus_tag="PWS53_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL0872421.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWS53_002219"
     CDS             complement(2881..3486)
                     /locus_tag="PWS53_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0872422.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWS53_002220"
     CDS             complement(3539..4513)
                     /locus_tag="PWS53_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL0872423.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWS53_002221"
     CDS             complement(4891..5610)
                     /locus_tag="PWS53_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0872424.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWS53_002222"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWS53_002222"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0872425.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWS53_002223"
     CDS             7161..8558
                     /locus_tag="PWS53_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0872426.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWS53_002224"
     CDS             8639..9079
                     /locus_tag="PWS53_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0872427.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWS53_002225"
     CDS             9227..9553
                     /locus_tag="PWS53_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0872428.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWS53_002226"
     CDS             9554..10303
                     /locus_tag="PWS53_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872429.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWS53_002227"
     CDS             complement(10383..10838)
                     /locus_tag="PWS53_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0872430.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11516)
                     /locus_tag="PWS53_002228"
     CDS             complement(11265..11516)
                     /locus_tag="PWS53_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872431.1"
                     /translation="MLKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKGIIYFLHEE
                     GYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3646 a         2003 c         2348 g         3542 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttca
//
LOCUS       DANJJH010000085        11445 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000085 DANJJH010000000
VERSION     DANJJH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            71..256
                     /locus_tag="PWS53_002229"
     CDS             71..256
                     /locus_tag="PWS53_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872432.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            268..516
                     /locus_tag="PWS53_002230"
     CDS             268..516
                     /locus_tag="PWS53_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872433.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            547..726
                     /locus_tag="PWS53_002231"
     CDS             547..726
                     /locus_tag="PWS53_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872434.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(769..1239)
                     /locus_tag="PWS53_002232"
     CDS             complement(769..1239)
                     /locus_tag="PWS53_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872435.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1326..2024
                     /locus_tag="PWS53_002233"
     CDS             1326..2024
                     /locus_tag="PWS53_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0872436.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2202..2543
                     /locus_tag="PWS53_002234"
     CDS             2202..2543
                     /locus_tag="PWS53_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872437.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2536..3207
                     /locus_tag="PWS53_002235"
     CDS             2536..3207
                     /locus_tag="PWS53_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0872438.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3213..3899
                     /locus_tag="PWS53_002236"
     CDS             3213..3899
                     /locus_tag="PWS53_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872439.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3902..4651
                     /locus_tag="PWS53_002237"
     CDS             3902..4651
                     /locus_tag="PWS53_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872440.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            4663..4932
                     /locus_tag="PWS53_002238"
     CDS             4663..4932
                     /locus_tag="PWS53_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872441.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            4937..5101
                     /locus_tag="PWS53_002239"
     CDS             4937..5101
                     /locus_tag="PWS53_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872442.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            5094..5396
                     /locus_tag="PWS53_002240"
     CDS             5094..5396
                     /locus_tag="PWS53_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872443.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            5393..5554
                     /locus_tag="PWS53_002241"
     CDS             5393..5554
                     /locus_tag="PWS53_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0872444.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            5551..5856
                     /locus_tag="PWS53_002242"
     CDS             5551..5856
                     /locus_tag="PWS53_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872445.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5856..6212
                     /locus_tag="PWS53_002243"
     CDS             5856..6212
                     /locus_tag="PWS53_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL0872446.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            6199..6417
                     /locus_tag="PWS53_002244"
     CDS             6199..6417
                     /locus_tag="PWS53_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872447.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            6414..6833
                     /locus_tag="PWS53_002245"
     CDS             6414..6833
                     /locus_tag="PWS53_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL0872448.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            6830..7387
                     /locus_tag="PWS53_002246"
     CDS             6830..7387
                     /locus_tag="PWS53_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL0872449.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            7384..7680
                     /locus_tag="PWS53_002247"
     CDS             7384..7680
                     /locus_tag="PWS53_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872450.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            7757..8170
                     /locus_tag="PWS53_002248"
     CDS             7757..8170
                     /locus_tag="PWS53_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL0872451.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            8516..8890
                     /locus_tag="PWS53_002249"
     CDS             8516..8890
                     /locus_tag="PWS53_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872452.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            8927..9094
                     /locus_tag="PWS53_002250"
     CDS             8927..9094
                     /locus_tag="PWS53_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872453.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            9120..9464
                     /locus_tag="PWS53_002251"
     CDS             9120..9464
                     /locus_tag="PWS53_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0872454.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9469..9750
                     /locus_tag="PWS53_002252"
     CDS             9469..9750
                     /locus_tag="PWS53_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872455.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9853..10167
                     /locus_tag="PWS53_002253"
     CDS             9853..10167
                     /locus_tag="PWS53_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL0872456.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10145..>11445
                     /locus_tag="PWS53_002254"
     CDS             10145..>11445
                     /locus_tag="PWS53_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0872457.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNK"
BASE COUNT         4252 a         1674 c         2456 g         3063 t
ORIGIN      
        1 tagttattgt caacgaatat tagctaattt tttagcttac agattatttt tttagaaagg
       61 agctattttt atgtctgaga atttagactt aaaaattcga gcggagatga gaaaaagaag
      121 aatgactttc aaagaactag ctgcgcttat cggtatttca ggagcttatt tatcagatat
      181 tctaaacggc aatcgtgatg gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat
      241 attggacatc cgataggggg gtaggcgatg acaaaactaa aaaaacaaga ttttgtaaaa
      301 aaatacaatt attctccatc tacttatcaa cgtcgaatgt cggaactaaa aaatacagca
      361 attttctcag cggcgtatga acgggtcaca ggacaagaag tttggatcaa tacagaatta
      421 tacgataaat ttttgtcttt caaatcctat aacaggttac gcacaagaaa ggtaacacct
      481 aaagaattta tcgagaagca tttagttgat ttataaaaaa cagaacattt tgagaggtga
      541 aggttaatgg gtaaattcaa cagagcattg gtattcagcg caccgctaat catctacgct
      601 ttaggacttt ggggaagcag gcaagcgttg ataggaacga ttgtttacat ggtttggatt
      661 tttatggggc ttgatgaagc tgaagctgag tacagagcga aaaagccaac cgagggggct
      721 gactaaaaat gaatagaaaa gagaaactag aatgtatatt actattactc agtttaattc
      781 tttcactaat ttctctattg ggaagtttgt atttttgata tcaacaattt tgtggatttg
      841 ggttctattt agagtaaata tcatttttgt ttggtgatta aggagtttat taaaattaat
      901 tttccctact tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa tatttagcgg
      961 tacagcttca gagtatattc tattatcatc gactgatcct cctttagcaa ttaatttctt
     1021 aaatggagta gctgaccatc tgcggtttaa gttagcggat gaaagttcta aattaactaa
     1081 catagctggt tctgttgaat agttagaaat gattactttt gtataaatca tatctgcagc
     1141 gaagtaagaa gcattaagtt caacttctag ttgagggcgc attcctttta tttttatccc
     1201 tgttaaaaac gtactcaata taaaacttat tattgacatc cattgaaaaa ttgttaagtg
     1261 aaaaaaattc aaaataaaca ccaccagttt ttaactaaat tataccaaaa aggagagaag
     1321 aaataatgca agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag
     1381 tggcagaaag cttcgagaaa aaacatcaac atgttttaag agatattgat gcactaaaaa
     1441 aagatgtgtc caattttgga cagatgtttg tagaaggtaa tgaaccagat tcatatggca
     1501 gaaatcgacg agttttcttc attagtagag atggattttt cttgctggct atggggttta
     1561 caggaaagaa agctatctac ttcaaacaaa aatacattga agcattcaac gaaatggaag
     1621 atgttattcg caagaatact gttcctcaaa caattgaaga tatgatgatc tatcaattag
     1681 aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg
     1741 gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatggga gttctagggg
     1801 gaaaagaaag ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg
     1861 aatttaaacg taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg
     1921 atgctgtttc atttattgaa atgtggttgc cagaaactgc gatccgtatg gaaattgatc
     1981 aactgaacag acaacagaga ctttttggtg atgacaatga atagagctga agcgctaaga
     2041 atagggacgg caattgctaa ttgctggtgg aaatactata aaccaatcat cctaagccaa
     2101 caacatattg acaagcaaaa agcatggcaa caaataaaaa agtgactccg ccggcaagca
     2161 aagagtcaca aagaaaacac atcataagga gattttagca tatggaaaaa gaactttcca
     2221 ctctagatca atatttgatt gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg
     2281 gtcgaaaaat cagacgaaat cttttgacga atgaagaact agcttgggat caagatgatt
     2341 taggcaacca tgtaactatt tgggatcatg tttatcttat ccatctatcg aagcattcga
     2401 ataaacctga atatatttac gtcatcgaag atggcttgat tgatgcgcta gaagagtacg
     2461 acagagataa cttgattgat atctcttatt acggaccagg taagaaatac attgctgaaa
     2521 tggaggcaga atttgatgag tgaaatcaaa gggacaacga actttgaaaa actttttagt
     2581 cgtaagttaa ataaaattct caagaaaaaa ggaaattttg attatttatc ttgggctcac
     2641 gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg taactattaa tgagtacaaa
     2701 cactacaggg ttgtttctgg aactcatcaa gactttcttg ttgaggaata taaacctttt
     2761 cttatggatg aaactgggac ttatgtatct gtctcagtaa cggttaaagg acacacggaa
     2821 accgaattat ttcctgtttt agattatcga aaccaaccag ttgttaaacc aaatgcaatg
     2881 caaatcaata actcattgaa gcgatgcttt gtgaaagcat tggctctaca cggactggga
     2941 ttatatgtat ttcaagggga agatattcca acaccaccta gaatcgatac aaagaaatta
     3001 aacatgctag agacgattct agaagctttc aatgagcaga tgggtaaaga tatgaccaaa
     3061 accttaatcg aatatgttaa tgagcagaca gataaattag ggctcttagc tgataacgtt
     3121 gaaactattg aacagttaag ctatgagcaa tgtgccttga tggagcgagc aatagcagct
     3181 aagagaaaag aattagataa gaagtgatat gagtgtttaa accattaatc gattcatatt
     3241 cagcggttct gaaaaagttc aaaggaaaag acataagcgc aaccatcaat gaggaagtga
     3301 acattgatcg actaaagacg atgtatgacg gctacgatgg tgatcgagtc attgaaattc
     3361 gttttattga tcctagacgt ttcaccgtac agcaacgaaa cttcatctat gcgctgatag
     3421 gcgatatttt tatcgataca ggcatgccaa cggacttctg gaaggaattc ttctacttcc
     3481 gttttgaagg tgtcacaggg cgcgaaataa gcctcaaaga cgaatcgaat acaaccgtga
     3541 gtgatgccaa tatcttagcg aatatcatcc tagatttcat ctttgaacat catattcctt
     3601 tcaaagaagg atatgagatt ttaccagcga atcaagagta ttacttctac aaatgcatta
     3661 caaaaagagt ctgctgcatc tgtggcaaaa caggagctga catcgatcac tttgacaaag
     3721 cgctaggaag acgaaagcgc aaagaagttg atcatgcaga gtacacattt gcagcactct
     3781 gcagaatcca tcacacagag aagcacaaaa taggtgtgat taatttcaaa aataaatatc
     3841 aaatcaaagg gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt ggagggtaaa
     3901 aatgacagaa catcgaagtt attatgcgat tataccagcc aacgtaaggt acgacaaaag
     3961 acttaaacca aatactaagt tgttatacgg agagataacg gccttgtgta atgaaagagg
     4021 cttttgttgg gcaggcaatg agtactttgc agatttatat ggtgtgaata aagagaccat
     4081 atcgcgatgg gtaagtgatt tgattaagtt tggatacttg aatcgggaaa tcatttacaa
     4141 agagggtacc aatcaaataa tcaataggta cctacgaatt aatcaatacc ctattgacga
     4201 aaaacgcaat acccctattg acgaaaaagt caaagataat aatacatcta ttaataatac
     4261 atttaataat acaaaagaat atataagaga gttaccgcct tcgaaaaaat cgaaggctaa
     4321 gcccgtccgt cataaatacg gagagtataa aaatgttctt ttgtcagatg agcagatgga
     4381 gaaactcaaa acagaattcc ctaatgatta ccaagagcga atcgaacgac tgtcagagta
     4441 ttgtgaatca tctggtaaga cttataaaaa ctatttggca actattcgaa gttgggcaag
     4501 gaaagaaaaa agtgaaccta agaacgcaag cagtggatac aagcgcacag gaagacgaga
     4561 gaagcttcct gaatgggcaa tcgaccaaga agcctatctc aagaaaaaag cgctagaacg
     4621 agctaataga caatcaaaag caccattcta agaggtggaa aaatgaagat cgattatcta
     4681 gaactaatta atgaaatagc gaattataaa aagggcgagg aattagacgt cctgagagac
     4741 gtatatgatc aactcgaaga agctggaatt gaaggaatta agaatgatcg ttcgagttgg
     4801 agtaagctca gatactattt cgcactttat atcgatacaa cacaattaag aaatttagca
     4861 tataccaaat tactatttgt tgattgtgtt aaaggcttgc aaaaacatct taatgaactt
     4921 gagcaggtgt aatcagatgg acctaaagac atttacagca cagattgaac taatgcatca
     4981 agaagcttta agacaaagtg tatcgtacga agacaagtgg ctcaacacgt tccatggcgg
     5041 acgtgagagc gcacttgatc aagtactcaa attactgaaa ggagaatgtc gggatggata
     5101 agaaagcggc aatgcagcga attatcgaat tgacttattc agaagattgg caaaatgaca
     5161 aagaagctgc ttcagaagtg atgaggcttg gaagagcgat gtgggcagac aagagcaaca
     5221 agccaagacc acgaaaaatc gcaatttggc acggtgacaa acttctagtg atagggacag
     5281 ctgaacagtt agcaagtctc acaggcttgc acgagaaaat cgtgaggaaa agagcaaggt
     5341 gtggctacac agacgctaag aagagaacgt ttagatacgt ggaggaatca tcatgacaac
     5401 agaagaagtg attcaaatgc gtattcgaag ccttcagcgt gagattgacg atctggaacg
     5461 aacaaaggca gtgatggtca atgaaacggc gagaaaggca atcgatttgc acatagagaa
     5521 tttaagaagg gaaatccatc gattggagga atgagcgtgg ataagaaagc agcaatgaaa
     5581 agaattgctg aattaaccaa gtcagaatct tggcaagaag acaaagaaat agttgcagaa
     5641 gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaaa
     5701 attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca gttatctgaa
     5761 attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg gattgattca
     5821 aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca caatacagtg
     5881 acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca aaagaacgag
     5941 ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt agcaggcatt
     6001 ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga
     6061 tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat cgtttcaaat
     6121 ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca aatgataaaa
     6181 aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac actatctgtg
     6241 gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt gtgggtagtt
     6301 gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc ggttgagttt
     6361 gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag cgaatgatac
     6421 cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc gtcttgcatt
     6481 ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc gaattgaaat
     6541 catatcacgt gcgaaacatc gacctcatgc aatccacagg aatgaaagat aagaatggtg
     6601 tggatatatt tgaaggggat atagtattag tcagcgtgcg aaatggcttc gattacttag
     6661 ataataaagt ctgtattgtc aaaaattcaa tagattattc cggattagtt tgtgccactg
     6721 ttgatgaaga cttagagtat caaattttta acacagagct gtttgaagaa tacacgtacg
     6781 aagtcaccgg aaatatatac gagaatagcg agttattgga ggaacagcga tgaataaaca
     6841 ggaagcaatc aaaaagttag aaagtattaa agcgatagga aatgatgcaa tagctgcttg
     6901 ctataacgag agtataaatt caggtattac gttaatgaaa aaaatagacg aaccgcagaa
     6961 accagttatt ccacaactcg tggccggttg gcttgagaaa tctacggacc cttttacaaa
     7021 agctgaaaaa atagcgtatt taatcaaatc taaagatggt gattcatatt atttctgtga
     7081 ttggtttgta cgagatggca tagtgacgca agagcaagga gaagaattac tcgcttgggc
     7141 aacaagacaa tcatatgaaa cactattgag cctatacaac ggctacgagg ttgagaaaga
     7201 gccgttatgg gcaataaaga atgccgatgg aaactatctt actaaatgtg ctttatgggg
     7261 aaaagatgga gtaaattata gttttgaatg caatccatct catcgattgc ttttcactga
     7321 taaagcaaca gcggatgctg cagcattgtt ggtgaatgga acagtggaag aggtggtaga
     7381 aagatgaaac taaaagacgg attttacgct agcagtcacg gtatcggcgg tttaatgcta
     7441 gatatgccga caaagaaccc taaaacacgc aagaaatcaa aattcaaagt cggtgacatg
     7501 gtccgctgtg aagcagaaga gttcgtttat ccgttcagag gatacgttaa aaagatactg
     7561 tcaaactcag caatcattcg tattgaaaat acaatgaagt gtgatcgttc gactgcaaaa
     7621 cacaagcatt atttagctgt agcgagatta gttgacatgg aagtaatcaa gagcaaataa
     7681 aaaaagccgg atcgctccga ctgattcaat aaatccaaca catttattat atcacataaa
     7741 ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga caagatgtaa
     7801 tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc gctctttgat
     7861 agatattaag tcgccgatta ttacggatat gccgaaggca ccaaagcacg gcaataaggc
     7921 agaagacgcg atcattcaga tgatggatat agaagcggag agagatgcga ttctagcggc
     7981 tttgatggct cttagtctaa ttagtcgcca gatactctac tacagcttct gtgtgccaga
     8041 tagcttctca aactacagga ttagccgtga agtgggttat tcagaaagaa gtatacaacg
     8101 gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa gaataattgc
     8161 ttataaataa tttggcggtt ttttggcgga atgatggcgg tttttagcta tttaccagtg
     8221 atattatggt agtgtcgaaa gattagtgat aggtctaaga caaaataaaa tgtaagggag
     8281 gaaatctccc tcatcgttta attaagcttc gatagacagc agcaaataaa ctaaaggatg
     8341 tgggggtcag ctcctacagt tagttcatgt gttgctgtct attaattgca actctttcgg
     8401 ttttattgag tatttactca acttaaaaaa actgttgttt gtagatgtaa gctgttccta
     8461 tttgtacata gtaaaaaatg atataataaa aatattacga aaggggtgag agtgcatgga
     8521 agacaaaatg tatttaagac aaaaagtttt cacaaaaaaa gatattgaaa atcaaattaa
     8581 tggatttata cagaaattag aaaatgcaaa ttcttatttg ataaataagg aactaaataa
     8641 agcttatgac cagtggaaag aagtatatga tgagttgaaa ttgattcaaa atgaaacaaa
     8701 attagtacga acggagaaaa agaatgaaaa tagtttcttt tttgatggat atgctatatt
     8761 gatgttggaa actgtagcaa aacaaaatat aaaagctccc aaaaaagaac tatctgacaa
     8821 tattgataac gcattagccg agttaaggta ttacgttatg caaataaaag atgttaggat
     8881 cactcgttga gtggtctttt tattttgcac aaaggaggta acaacaatgt atagaccaca
     8941 atacttagaa cagaagtatg aagtaatcac tgtgcaaaat ggtaacggag agatagtacg
     9001 aaagtataga agaccaataa agagcgatac atataaacga aaggaaagca atgaagttat
     9061 tccattgtat ggcaaaagaa tagctaagca ttaaataaga ttgtgaaagg agacgggaca
     9121 tgaacgagga attctataga tggctattac agttgataag agaagatcgt ttggttaagt
     9181 tctatcagtc tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg
     9241 aatgccaaga gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa
     9301 aggaagtcaa ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg
     9361 ttgaacatca taatgaagtt catggcagat atcttacagc attggataaa caagagaaga
     9421 agatagaaag ctttgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat
     9481 gataatggca gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga
     9541 ttaattaaac gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac
     9601 tgttgtagta aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc
     9661 gaaagaataa aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc
     9721 gctgaagctt tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt
     9781 gggggtgatg aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa
     9841 aggagggcta aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc
     9901 cgctctttaa taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat
     9961 gttgatcgtt atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag
    10021 aacggcatta gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc
    10081 aacaaacaaa tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa
    10141 gcaaatgaat caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga
    10201 aggcactatc aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt
    10261 ctttactaga gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc
    10321 agcacaatac tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta
    10381 tggtgttcga tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg
    10441 acgtggtaat ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa
    10501 acacggtatt aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac
    10561 tagttttgat gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt
    10621 taaagctacg aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac
    10681 ggcaaatgct agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca
    10741 cgaaaatgaa gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga
    10801 ttatagagaa ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat
    10861 tatagaagaa tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga
    10921 attttctagt ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc
    10981 cgacatgtgg gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat
    11041 gtttcaagaa tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa
    11101 acgaatgaac agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct
    11161 gcatacgaaa gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt
    11221 tgctgataga cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta
    11281 ttttacacaa catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga
    11341 ggttatagat atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga
    11401 agctgattat attgtaggtt ggtttcttga aatgagtaat aaata
//
LOCUS       DANJJH010000086        11317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000086 DANJJH010000000
VERSION     DANJJH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(563..787)
                     /locus_tag="PWS53_002255"
     CDS             complement(563..787)
                     /locus_tag="PWS53_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872458.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            978..3755
                     /locus_tag="PWS53_002256"
     CDS             978..3755
                     /locus_tag="PWS53_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0872459.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3836..4255
                     /locus_tag="PWS53_002257"
     CDS             3836..4255
                     /locus_tag="PWS53_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL0872460.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4267..4737
                     /locus_tag="PWS53_002258"
     CDS             4267..4737
                     /locus_tag="PWS53_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872461.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4754..5533
                     /locus_tag="PWS53_002259"
     CDS             4754..5533
                     /locus_tag="PWS53_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0872462.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5520..6341
                     /locus_tag="PWS53_002260"
     CDS             5520..6341
                     /locus_tag="PWS53_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0872463.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6358..7407
                     /locus_tag="PWS53_002261"
     CDS             6358..7407
                     /locus_tag="PWS53_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0872464.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7420..8424
                     /locus_tag="PWS53_002262"
     CDS             7420..8424
                     /locus_tag="PWS53_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0872465.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9057..10076
                     /locus_tag="PWS53_002263"
     CDS             9057..10076
                     /locus_tag="PWS53_002263"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0872466.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(10242..>11317)
                     /locus_tag="PWS53_002264"
     CDS             complement(10242..>11317)
                     /locus_tag="PWS53_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0872467.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3729 a         1872 c         2340 g         3376 t
ORIGIN      
        1 atttttctat aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac
       61 tctatttact tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa
      121 aaagtgggtt gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat
      181 atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg
      241 gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa
      301 aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt
      361 ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa
      421 aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca
      481 ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca
      541 ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca
      601 agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac
      661 gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag
      721 atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg
      781 atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa
      841 ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct
      901 ataataagat atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt
      961 tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga
     1021 accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag
     1081 ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg
     1141 agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg
     1201 aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg
     1261 aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca
     1321 gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac
     1381 cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc
     1441 agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg
     1501 cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag
     1561 cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct
     1621 tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc
     1681 ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac
     1741 gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa
     1801 gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag
     1861 aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca
     1921 gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga
     1981 aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa
     2041 tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata
     2101 cagcacagca aggaatctat attacgcaag accagacctt agagcaatta atggagaagc
     2161 aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga
     2221 aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat
     2281 ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga
     2341 cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg
     2401 gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac
     2461 cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt
     2521 atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata
     2581 ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt
     2641 caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca
     2701 gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc
     2761 cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg
     2821 atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt
     2881 tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag
     2941 caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga
     3001 ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag
     3061 cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag
     3121 aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa
     3181 aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca
     3241 ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg
     3301 aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa
     3361 tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta
     3421 ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc
     3481 agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc
     3541 tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga
     3601 agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc
     3661 attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag
     3721 atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat
     3781 tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga
     3841 tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag attcattaga
     3901 gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt
     3961 tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt
     4021 tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga
     4081 tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt
     4141 ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat
     4201 gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga
     4261 agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt
     4321 tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg
     4381 aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc
     4441 aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta
     4501 ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc
     4561 aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg
     4621 gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa
     4681 gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa
     4741 aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca
     4801 atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac
     4861 aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc
     4921 atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat
     4981 tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg
     5041 tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc
     5101 aattatgaac cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca
     5161 tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc
     5221 ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca
     5281 aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg
     5341 tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta
     5401 cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt
     5461 caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga
     5521 tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt
     5581 ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg
     5641 tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag
     5701 ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag
     5761 gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga
     5821 tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt
     5881 ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata
     5941 ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc
     6001 tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg
     6061 gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa
     6121 tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg
     6181 tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg
     6241 gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag
     6301 ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg
     6361 tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag
     6421 aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg
     6481 ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt
     6541 actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc
     6601 gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa
     6661 atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt
     6721 gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt
     6781 acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg aattggaata
     6841 agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata
     6901 atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt
     6961 ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct
     7021 aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta
     7081 gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt
     7141 gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac
     7201 gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta
     7261 gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc
     7321 gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt
     7381 gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa
     7441 gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc
     7501 gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat
     7561 gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta
     7621 cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt
     7681 attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag
     7741 aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta
     7801 tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca
     7861 gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag
     7921 ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc
     7981 ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat
     8041 caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca
     8101 tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca
     8161 accccagtca cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct
     8221 aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta
     8281 gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg
     8341 acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc
     8401 tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag
     8461 agcagcgata actgctccct ttattttttt aaaatattgg catcaattac gttataaaac
     8521 acattttttg cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc
     8581 aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc
     8641 caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac
     8701 aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt
     8761 tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc
     8821 taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa
     8881 gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt
     8941 caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac
     9001 tttaagaaat gtatactata atggaaatta tcaattattt atttggagga atatccatga
     9061 aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg
     9121 cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca
     9181 ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg
     9241 gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa
     9301 caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca
     9361 ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg
     9421 ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg
     9481 ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag
     9541 cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg
     9601 atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg
     9661 ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg
     9721 cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga
     9781 gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg
     9841 gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg
     9901 ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga
     9961 tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag
    10021 caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag
    10081 atgatcaata gttaatcgtc gtttagtcta atggtgataa gtcaagatga aatttaagat
    10141 tttctttatt gagtaatatg ttttgaagcg cacgaaacta gacccgatca aaatcaaatt
    10201 tttttcgggt tgattcaagg atttgcgtca ataacggatt gttatttctc tatcacactc
    10261 cttggtggcg tatagagttg gtgtttacgt agtagcgtat ccaccagacg cgtaaatttt
    10321 cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg gaacctcctt atatttactt
    10381 tgataaaaag ctttgtattc aggaagataa ttttttacag agttggcagc ttcaactaag
    10441 tagtatctaa aatatcgatt tccttgtttt gtcagaggtg tattttgcga ctgatagttt
    10501 ccagattggt tcactttcca acttaatcca gcgtattttg ccaattttgt ctgatcttca
    10561 aatctttcga tttgaccaat ttcagcgatt aaaccggcag catagacttt accaacgcct
    10621 ggaatactgg ttaagcattg atattctgga atgacaacaa ttacttgttc gatggcttta
    10681 tctaattctt tgatattttt ttcaagtgct cgtatttctc gtacgagaac acttaaaaca
    10741 acattaattg attcttgtgc aagagcacct aggcggtaac tcatggtaat tgcacgttga
    10801 attgctttag cgattttttc tggttgcttg aaacggcctt ttcctttttc ttgaagtaaa
    10861 tcacagaaag cttctaaagg aagctcagca agttcatcca atgtgaaatc ttccgtcatg
    10921 agtgaaatga gggttgcact aaaaagactc gtcgattcgt ctcgcatttc tcgagcaagc
    10981 gtattacatt tgtacgttag attttcaaga aaatgttgct ttgtacgaat caattgctta
    11041 ataagttgat agcgagtacg tgttaaacgc tgtaaggcca tgtatttctc ttctttgata
    11101 ggggaagttg tgaatcgttg aatacgcaag aaatcagcga ttcttaatgc atcgtttcga
    11161 tcagttttat tctcatcaaa catgcgacta aattgtttca cccgaaaagg attttcaatc
    11221 gttacaagtg tgttgagctt ctttagtttc tcatcttcat gaaaaaacat ggaaggatgg
    11281 aagctgtaca tggacgttga ctccatgcca atcacaa
//
LOCUS       DANJJH010000087        11089 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000087 DANJJH010000000
VERSION     DANJJH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            51..1310
                     /gene="lysA"
                     /locus_tag="PWS53_002265"
     CDS             51..1310
                     /gene="lysA"
                     /locus_tag="PWS53_002265"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL0872468.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1297..2097
                     /gene="dapF"
                     /locus_tag="PWS53_002266"
     CDS             1297..2097
                     /gene="dapF"
                     /locus_tag="PWS53_002266"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL0872469.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2100..2981
                     /locus_tag="PWS53_002267"
     CDS             2100..2981
                     /locus_tag="PWS53_002267"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL0872470.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2978..3694
                     /locus_tag="PWS53_002268"
     CDS             2978..3694
                     /locus_tag="PWS53_002268"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL0872471.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3700..4395
                     /gene="dapD"
                     /locus_tag="PWS53_002269"
     CDS             3700..4395
                     /gene="dapD"
                     /locus_tag="PWS53_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL0872472.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4411..5412
                     /locus_tag="PWS53_002270"
     CDS             4411..5412
                     /locus_tag="PWS53_002270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL0872473.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5409..6770
                     /locus_tag="PWS53_002271"
     CDS             5409..6770
                     /locus_tag="PWS53_002271"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL0872474.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6730..7881
                     /locus_tag="PWS53_002272"
     CDS             6730..7881
                     /locus_tag="PWS53_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0872475.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7856..9025
                     /locus_tag="PWS53_002273"
     CDS             7856..9025
                     /locus_tag="PWS53_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0872476.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9200..10579
                     /locus_tag="PWS53_002274"
     CDS             9200..10579
                     /locus_tag="PWS53_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0872477.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10860..11089
                     /locus_tag="PWS53_002275"
                     /pseudo
     CDS             10860..11089
                     /locus_tag="PWS53_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3687 a         1941 c         2494 g         2967 t
ORIGIN      
        1 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
       61 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
      121 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
      181 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
      241 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
      301 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
      361 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
      421 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
      481 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
      541 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
      601 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
      661 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
      721 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
      781 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
      841 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
      901 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
      961 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     1021 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     1081 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     1141 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     1201 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     1261 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     1321 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     1381 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     1441 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     1501 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     1561 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     1621 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     1681 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     1741 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     1801 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     1861 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     1921 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     1981 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     2041 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     2101 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     2161 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     2221 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     2281 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     2341 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     2401 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     2461 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     2521 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     2581 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     2641 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     2701 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     2761 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     2821 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     2881 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     2941 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     3001 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     3061 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     3121 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     3181 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     3241 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     3301 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     3361 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     3421 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     3481 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     3541 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     3601 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     3661 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     3721 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     3781 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     3841 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     3901 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     3961 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     4021 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     4081 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     4141 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     4201 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     4261 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     4321 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     4381 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     4441 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     4501 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     4561 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     4621 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     4681 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     4741 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     4801 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     4861 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     4921 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     4981 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     5041 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     5101 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     5161 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     5221 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     5281 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     5341 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     5401 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     5461 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     5521 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     5581 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     5641 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     5701 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     5761 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     5821 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     5881 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     5941 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     6001 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     6061 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     6121 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     6181 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     6241 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     6301 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     6361 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     6421 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     6481 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     6541 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     6601 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     6661 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     6721 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     6781 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     6841 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     6901 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     6961 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     7021 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     7081 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     7141 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     7201 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     7261 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     7321 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     7381 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     7441 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     7501 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     7561 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     7621 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
     7681 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
     7741 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
     7801 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
     7861 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
     7921 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
     7981 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
     8041 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
     8101 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
     8161 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
     8221 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
     8281 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
     8341 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
     8401 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
     8461 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
     8521 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
     8581 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
     8641 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
     8701 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
     8761 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
     8821 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
     8881 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
     8941 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
     9001 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
     9061 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
     9121 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
     9181 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
     9241 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
     9301 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
     9361 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
     9421 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
     9481 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
     9541 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
     9601 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
     9661 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
     9721 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
     9781 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
     9841 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
     9901 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
     9961 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    10021 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    10081 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    10141 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    10201 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    10261 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    10321 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    10381 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    10441 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    10501 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    10561 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    10621 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    10681 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    10741 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    10801 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    10861 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    10921 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    10981 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    11041 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DANJJH010000088        10922 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000088 DANJJH010000000
VERSION     DANJJH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..281)
                     /locus_tag="PWS53_002276"
     CDS             complement(<1..281)
                     /locus_tag="PWS53_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872478.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="PWS53_002277"
     CDS             423..1541
                     /locus_tag="PWS53_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0872479.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="PWS53_002278"
     CDS             1669..3045
                     /locus_tag="PWS53_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0872480.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="PWS53_002279"
     CDS             3446..5026
                     /locus_tag="PWS53_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL0872481.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="PWS53_002280"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="PWS53_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="PWS53_002281"
     CDS             complement(5652..5972)
                     /locus_tag="PWS53_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL0872482.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="PWS53_002282"
     CDS             complement(6120..8354)
                     /locus_tag="PWS53_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0872483.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="PWS53_002283"
     CDS             8833..9099
                     /locus_tag="PWS53_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL0872484.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="PWS53_002284"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="PWS53_002284"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL0872485.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DANJJH010000089        10817 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000089 DANJJH010000000
VERSION     DANJJH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(25..162)
                     /locus_tag="PWS53_002285"
     CDS             complement(25..162)
                     /locus_tag="PWS53_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0872486.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="PWS53_002286"
     CDS             complement(159..1343)
                     /locus_tag="PWS53_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0872487.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="PWS53_002287"
     CDS             complement(1599..1853)
                     /locus_tag="PWS53_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872488.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="PWS53_002288"
     CDS             complement(1878..3020)
                     /locus_tag="PWS53_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL0872489.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="PWS53_002289"
     CDS             complement(3080..4432)
                     /locus_tag="PWS53_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0872490.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="PWS53_002290"
     CDS             complement(4503..4847)
                     /locus_tag="PWS53_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0872491.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="PWS53_002291"
     CDS             complement(4857..5231)
                     /locus_tag="PWS53_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0872492.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="PWS53_002292"
     CDS             complement(5244..5558)
                     /locus_tag="PWS53_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0872493.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWS53_002293"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="PWS53_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="PWS53_002294"
     CDS             complement(8976..9638)
                     /locus_tag="PWS53_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872494.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="PWS53_002295"
     CDS             10255..10485
                     /locus_tag="PWS53_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872495.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="PWS53_002296"
     CDS             10491..10790
                     /locus_tag="PWS53_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872496.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DANJJH010000090        10724 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000090 DANJJH010000000
VERSION     DANJJH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(241..1089)
                     /locus_tag="PWS53_002297"
     CDS             complement(241..1089)
                     /locus_tag="PWS53_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0872497.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="PWS53_002298"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="PWS53_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL0872498.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="PWS53_002299"
     CDS             2157..3158
                     /locus_tag="PWS53_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL0872499.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="PWS53_002300"
     CDS             3155..3439
                     /locus_tag="PWS53_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL0872500.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="PWS53_002301"
     CDS             3472..4269
                     /locus_tag="PWS53_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0872501.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="PWS53_002302"
     CDS             4283..5116
                     /locus_tag="PWS53_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0872502.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="PWS53_002303"
     CDS             5134..5991
                     /locus_tag="PWS53_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0872503.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="PWS53_002304"
     CDS             6013..6522
                     /locus_tag="PWS53_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872504.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="PWS53_002305"
     CDS             6559..6960
                     /locus_tag="PWS53_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872505.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="PWS53_002306"
     CDS             7072..7425
                     /locus_tag="PWS53_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872506.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="PWS53_002307"
     CDS             7459..7902
                     /locus_tag="PWS53_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0872507.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="PWS53_002308"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="PWS53_002308"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0872508.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="PWS53_002309"
     CDS             8997..9710
                     /locus_tag="PWS53_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0872509.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="PWS53_002310"
     CDS             complement(9917..10066)
                     /locus_tag="PWS53_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872510.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="PWS53_002311"
     CDS             10462..10662
                     /locus_tag="PWS53_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0872511.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DANJJH010000091        10555 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000091 DANJJH010000000
VERSION     DANJJH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(148..2301)
                     /locus_tag="PWS53_002312"
     CDS             complement(148..2301)
                     /locus_tag="PWS53_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL0872512.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2595..3443)
                     /locus_tag="PWS53_002313"
     CDS             complement(2595..3443)
                     /locus_tag="PWS53_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0872513.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="PWS53_002314"
     CDS             complement(3667..5607)
                     /gene="thrS"
                     /locus_tag="PWS53_002314"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL0872514.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5944..6123)
                     /locus_tag="PWS53_002315"
     CDS             complement(5944..6123)
                     /locus_tag="PWS53_002315"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL0872515.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="PWS53_002316"
     CDS             complement(6170..7993)
                     /gene="cadA"
                     /locus_tag="PWS53_002316"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0872516.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8271..10550
                     /locus_tag="PWS53_002317"
     CDS             8271..10550
                     /locus_tag="PWS53_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0872517.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         3022 a         2256 c         1888 g         3389 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc tttacctaat
     2401 ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat atccaactat
     2461 ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt cgtgaaacac
     2521 tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa cttatataaa
     2581 aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc tcgtgttttt
     2641 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
     2701 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
     2761 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
     2821 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
     2881 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
     2941 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
     3001 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
     3061 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
     3121 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
     3181 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
     3241 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
     3301 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
     3361 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
     3421 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
     3481 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
     3541 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
     3601 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     3661 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     3721 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     3781 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     3841 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     3901 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     3961 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     4021 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     4081 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     4141 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     4201 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     4261 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     4321 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     4381 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     4441 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     4501 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     4561 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     4621 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     4681 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     4741 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     4801 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     4861 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     4921 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     4981 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     5041 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     5101 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     5161 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     5221 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     5281 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     5341 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     5401 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     5461 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     5521 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     5581 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     5641 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     5701 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     5761 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     5821 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     5881 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     5941 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     6001 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     6061 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     6121 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     6181 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     6241 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     6301 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     6361 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     6421 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     6481 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     6541 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     6601 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     6661 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     6721 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     6781 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     6841 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     6901 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     6961 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     7021 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     7081 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     7141 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     7201 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     7261 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     7321 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     7381 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     7441 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     7501 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     7561 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     7621 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     7681 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     7741 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     7801 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     7861 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     7921 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     7981 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     8041 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     8101 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     8161 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     8221 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     8281 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     8341 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     8401 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     8461 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     8521 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     8581 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     8641 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     8701 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     8761 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     8821 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     8881 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     8941 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     9001 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     9061 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     9121 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     9181 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     9241 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     9301 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     9361 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     9421 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     9481 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     9541 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     9601 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     9661 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     9721 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     9781 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     9841 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     9901 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     9961 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
    10021 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
    10081 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
    10141 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
    10201 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
    10261 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
    10321 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
    10381 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
    10441 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
    10501 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaa
//
LOCUS       DANJJH010000092        10385 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000092 DANJJH010000000
VERSION     DANJJH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            134..283
                     /locus_tag="PWS53_002318"
     CDS             134..283
                     /locus_tag="PWS53_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL0872518.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            294..1808
                     /gene="dltA"
                     /locus_tag="PWS53_002319"
     CDS             294..1808
                     /gene="dltA"
                     /locus_tag="PWS53_002319"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL0872519.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1805..3010
                     /gene="dltB"
                     /locus_tag="PWS53_002320"
     CDS             1805..3010
                     /gene="dltB"
                     /locus_tag="PWS53_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL0872520.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3055..3288
                     /gene="dltC"
                     /locus_tag="PWS53_002321"
     CDS             3055..3288
                     /gene="dltC"
                     /locus_tag="PWS53_002321"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL0872521.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3291..4559
                     /gene="dltD"
                     /locus_tag="PWS53_002322"
     CDS             3291..4559
                     /gene="dltD"
                     /locus_tag="PWS53_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL0872522.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4700..5782
                     /locus_tag="PWS53_002323"
     CDS             4700..5782
                     /locus_tag="PWS53_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0872523.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5782..6585
                     /locus_tag="PWS53_002324"
     CDS             5782..6585
                     /locus_tag="PWS53_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0872524.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6655..7395
                     /locus_tag="PWS53_002325"
     CDS             6655..7395
                     /locus_tag="PWS53_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0872525.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7406..8410
                     /locus_tag="PWS53_002326"
     CDS             7406..8410
                     /locus_tag="PWS53_002326"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL0872526.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8455..10299)
                     /locus_tag="PWS53_002327"
     CDS             complement(8455..10299)
                     /locus_tag="PWS53_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872527.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3345 a         1710 c         2092 g         3238 t
ORIGIN      
        1 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
       61 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
      121 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
      181 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
      241 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
      301 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
      361 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
      421 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
      481 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
      541 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
      601 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
      661 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
      721 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
      781 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
      841 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
      901 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
      961 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
     1021 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
     1081 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
     1141 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
     1201 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
     1261 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
     1321 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
     1381 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
     1441 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
     1501 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
     1561 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
     1621 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
     1681 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
     1741 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
     1801 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
     1861 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
     1921 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
     1981 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
     2041 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
     2101 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
     2161 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
     2221 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
     2281 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
     2341 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
     2401 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
     2461 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
     2521 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
     2581 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
     2641 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
     2701 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
     2761 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
     2821 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
     2881 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
     2941 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
     3001 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
     3061 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
     3121 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
     3181 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
     3241 agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
     3301 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
     3361 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
     3421 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
     3481 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
     3541 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
     3601 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
     3661 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
     3721 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
     3781 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
     3841 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
     3901 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
     3961 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
     4021 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
     4081 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
     4141 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
     4201 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
     4261 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
     4321 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
     4381 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
     4441 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
     4501 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
     4561 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
     4621 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
     4681 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
     4741 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
     4801 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
     4861 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
     4921 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
     4981 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
     5041 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
     5101 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
     5161 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
     5221 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
     5281 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
     5341 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
     5401 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
     5461 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
     5521 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
     5581 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
     5641 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
     5701 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
     5761 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
     5821 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
     5881 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
     5941 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
     6001 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
     6061 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
     6121 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
     6181 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
     6241 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
     6301 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
     6361 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
     6421 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
     6481 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
     6541 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
     6601 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
     6661 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
     6721 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
     6781 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
     6841 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
     6901 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
     6961 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
     7021 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
     7081 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
     7141 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
     7201 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
     7261 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
     7321 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
     7381 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
     7441 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
     7501 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
     7561 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
     7621 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
     7681 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
     7741 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
     7801 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
     7861 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
     7921 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
     7981 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
     8041 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
     8101 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
     8161 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
     8221 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
     8281 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
     8341 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
     8401 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
     8461 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
     8521 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
     8581 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
     8641 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
     8701 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
     8761 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
     8821 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
     8881 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
     8941 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
     9001 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
     9061 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
     9121 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
     9181 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
     9241 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
     9301 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
     9361 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
     9421 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
     9481 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
     9541 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
     9601 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
     9661 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
     9721 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
     9781 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
     9841 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
     9901 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
     9961 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    10021 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    10081 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    10141 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    10201 tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac gtaaataaaa
    10261 agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc tttctaaaac
    10321 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
    10381 ataaa
//
LOCUS       DANJJH010000093        10334 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000093 DANJJH010000000
VERSION     DANJJH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            257..532
                     /locus_tag="PWS53_002328"
     CDS             257..532
                     /locus_tag="PWS53_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872528.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="PWS53_002329"
     CDS             545..2617
                     /locus_tag="PWS53_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL0872529.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="PWS53_002330"
     CDS             2782..4668
                     /locus_tag="PWS53_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0872530.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="PWS53_002331"
     CDS             4680..5627
                     /locus_tag="PWS53_002331"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL0872531.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="PWS53_002332"
     CDS             5647..6174
                     /locus_tag="PWS53_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0872532.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="PWS53_002333"
     CDS             complement(6237..6890)
                     /locus_tag="PWS53_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL0872533.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="PWS53_002334"
     CDS             7022..7864
                     /locus_tag="PWS53_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0872534.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="PWS53_002335"
     CDS             8022..8918
                     /locus_tag="PWS53_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0872535.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="PWS53_002336"
     CDS             8921..9634
                     /locus_tag="PWS53_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL0872536.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="PWS53_002337"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="PWS53_002337"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0872537.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..>10334
                     /locus_tag="PWS53_002338"
     CDS             10169..>10334
                     /locus_tag="PWS53_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL0872538.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYL"
BASE COUNT         3581 a         1766 c         2141 g         2846 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgc
//
LOCUS       DANJJH010000094        10193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000094 DANJJH010000000
VERSION     DANJJH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            120..626
                     /locus_tag="PWS53_002339"
     CDS             120..626
                     /locus_tag="PWS53_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL0872539.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="PWS53_002340"
     CDS             643..1542
                     /locus_tag="PWS53_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL0872540.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="PWS53_002341"
     CDS             1629..2513
                     /locus_tag="PWS53_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0872541.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="PWS53_002342"
     tRNA            complement(2705..2776)
                     /locus_tag="PWS53_002342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="PWS53_002343"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="PWS53_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL0872542.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="PWS53_002344"
     CDS             4319..5410
                     /locus_tag="PWS53_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL0872543.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="PWS53_002345"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="PWS53_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0872544.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="PWS53_002346"
     CDS             6562..7752
                     /locus_tag="PWS53_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL0872545.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="PWS53_002347"
     CDS             7856..8611
                     /locus_tag="PWS53_002347"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL0872546.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="PWS53_002348"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="PWS53_002348"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL0872547.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DANJJH010000095        10086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000095 DANJJH010000000
VERSION     DANJJH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(247..759)
                     /locus_tag="PWS53_002349"
     CDS             complement(247..759)
                     /locus_tag="PWS53_002349"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL0872548.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWS53_002350"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="PWS53_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL0872549.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="PWS53_002351"
     CDS             complement(3212..3655)
                     /locus_tag="PWS53_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL0872550.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="PWS53_002352"
     CDS             complement(3656..4444)
                     /locus_tag="PWS53_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0872551.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="PWS53_002353"
     CDS             complement(4441..5382)
                     /locus_tag="PWS53_002353"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL0872552.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="PWS53_002354"
     CDS             complement(5559..6146)
                     /locus_tag="PWS53_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL0872553.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWS53_002355"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="PWS53_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL0872554.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="PWS53_002356"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="PWS53_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0872555.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="PWS53_002357"
     CDS             complement(9063..9998)
                     /locus_tag="PWS53_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0872556.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DANJJH010000096        10084 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000096 DANJJH010000000
VERSION     DANJJH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            162..392
                     /locus_tag="PWS53_002358"
     CDS             162..392
                     /locus_tag="PWS53_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL0872557.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="PWS53_002359"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="PWS53_002359"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0872558.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="PWS53_002360"
     CDS             1803..1979
                     /locus_tag="PWS53_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0872559.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="PWS53_002361"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="PWS53_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL0872560.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="PWS53_002362"
     CDS             2474..2941
                     /locus_tag="PWS53_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872561.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="PWS53_002363"
     CDS             3026..3910
                     /locus_tag="PWS53_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0872562.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="PWS53_002364"
     CDS             4024..4260
                     /locus_tag="PWS53_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL0872563.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="PWS53_002365"
     tRNA            4348..4428
                     /locus_tag="PWS53_002365"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="PWS53_002366"
     tRNA            4435..4506
                     /locus_tag="PWS53_002366"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="PWS53_002367"
     CDS             4631..4801
                     /locus_tag="PWS53_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872564.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="PWS53_002368"
     CDS             5021..5728
                     /locus_tag="PWS53_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0872565.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="PWS53_002369"
     CDS             complement(5788..6432)
                     /locus_tag="PWS53_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL0872566.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="PWS53_002370"
     CDS             6559..6846
                     /locus_tag="PWS53_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872567.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="PWS53_002371"
     CDS             6954..7274
                     /locus_tag="PWS53_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872568.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="PWS53_002372"
     CDS             7355..8668
                     /locus_tag="PWS53_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0872569.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="PWS53_002373"
     CDS             8665..9384
                     /locus_tag="PWS53_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL0872570.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="PWS53_002374"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="PWS53_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL0872571.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DANJJH010000097        10051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000097 DANJJH010000000
VERSION     DANJJH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1738)
                     /locus_tag="PWS53_002375"
     CDS             complement(<1..1738)
                     /locus_tag="PWS53_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL0872572.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDA"
     gene            complement(1751..2545)
                     /locus_tag="PWS53_002376"
     CDS             complement(1751..2545)
                     /locus_tag="PWS53_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0872573.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(2556..3932)
                     /locus_tag="PWS53_002377"
     CDS             complement(2556..3932)
                     /locus_tag="PWS53_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0872574.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(4061..4708)
                     /locus_tag="PWS53_002378"
     CDS             complement(4061..4708)
                     /locus_tag="PWS53_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL0872575.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(4792..6219)
                     /gene="uxaC"
                     /locus_tag="PWS53_002379"
     CDS             complement(4792..6219)
                     /gene="uxaC"
                     /locus_tag="PWS53_002379"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0872576.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(6293..7372)
                     /gene="uxuA"
                     /locus_tag="PWS53_002380"
     CDS             complement(6293..7372)
                     /gene="uxuA"
                     /locus_tag="PWS53_002380"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0872577.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(7375..9006)
                     /locus_tag="PWS53_002381"
     CDS             complement(7375..9006)
                     /locus_tag="PWS53_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0872578.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9323..9994
                     /locus_tag="PWS53_002382"
     CDS             9323..9994
                     /locus_tag="PWS53_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0872579.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSRNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         3220 a         2000 c         1329 g         3502 t
ORIGIN      
        1 aagcatctat tggacttatt cccttaaatt cagaaaggcc cattagtttg tccactaata
       61 tttctaaagt ttcttgtttt ccatcataag tattgatata agtttttact tgaggcacat
      121 cagctaaatg aaacggacac tgtacagata caaaaattgt tggaatttca tgaatataaa
      181 atggaatatc cggagtacct ttacttgctg gccaaacaat tctttgaact gtacctgggt
      241 taacattagc tatattaata atcaagtcat atttttcagt taaatctttg attggtctct
      301 tagcagcata tattccagct atatttttaa ttttttcttc ttgcgataat cctttcaatt
      361 cttcttcagg tgaaatgtat ttttcaactt caaatccttt tgctgacaaa atctgtttca
      421 ataaatcaat ggcctgaggt ctatttccag caactacttc accaaaccca ccttttaccc
      481 catgtacatc taccagtaaa atccttttat atttttctgg tacaataggc caaatatctt
      541 gtttatcttt aactagtgta attgcttgat cagctacctc tttaaaaatt gatttatttt
      601 caattaaacc aattttttcc atagcttctt gtttcggtaa caaaatttta ttcttttctt
      661 ttttatgcaa acctagttta gcctttaagc ctaaaattct tcttaatgca tcacgtaaac
      721 gctcttctga aataattcct ttgtaataac cttccatcat ataattaaag tcttcatctg
      781 gatcattaaa aaataggaat agatcacatc cagccgcaat agaagtgggc aataattctg
      841 accgtttcat tgccccagtc attcctaaca tatgactagc atcagtaact actaatccat
      901 taaacccgag ttttcctcta agtaagtcag tgattaattc ttttgataaa gttgcaggaa
      961 gtaaatcttc atctttcaga tcaagattaa aatgttttga ataagctggc atatggatat
     1021 gacctgccat aattgaaggt aatccagctt ctattaagcc tgagtatact tttccaaaag
     1081 aattatccca ttcttctgta cttaaactat taatactaaa agatagatgt tgatctcgtt
     1141 cgtcaatacc gtcaccaggg aaatgtttag ctgcaggcaa aattccactc tcttgaatac
     1201 ctttcatata agctaaacta agttctaaaa ctttatcagg gctttgtcca aaagatcgac
     1261 tagaaatgat aggatttctc caatttctat taatatctac aataggagca aaactccagt
     1321 tacatccaat agccgatgct tcaattccag aaactctccc taattcataa gcatatttcg
     1381 catcatttgt tgcaccaatt tttacttcta caccaacttc ggtaccatct gtacaagctc
     1441 cgttaccacc tgcttctgta ttagcagcaa ttaataaagg aattttgctt tctgtttgta
     1501 ggatatagtt ttgatcatat acctcatcag cagttcctgg attatatcgt acagctccaa
     1561 tatggtactg attgaccata ctacttaagt aatttttatc acgactagat cccatattaa
     1621 caaataattg acctattttt tcttctaaag tcatagcatc aatcgtctct tcaatccaag
     1681 aaatagcatc ttcatctaaa tgatatggtt tttttcgtaa gtctactttt atagtcattt
     1741 tctttctcct ctacttatta gtaagatttt tccaaaaagt atctatatat ttttgttcaa
     1801 tatctatata ctctcccgat aaacatttaa ataaaatatc agttcttaag ttttcccatg
     1861 attctttttc tttattaacc aaaataggat aaaagaaaac tttattctta tttgcagctt
     1921 ctagatcacc aggagaatca ccaatcatta atatcttatc caaatcacaa ccattttcaa
     1981 ttacacttcg aataatgtct tctttttttc ctttatcttg acaacacagt tcatcaacta
     2041 aggataataa ttcatgtctt tcccattctt cttgaatagc ttccttatta gctgagctta
     2101 caataattat cttcccatat tttttcagag cttttatagt atctctagca cttaaaaagg
     2161 gtttatctaa tcctttagct tgactaatct gtcgattgac ttcttccgac cattttaaag
     2221 cttttaataa atcctctgac tgagagtatt ctatttccct cttcaaagat ggattagaca
     2281 attcttttgt tacagatacc cagtgcttta atcgattaat cgatggtaca tcattttttt
     2341 ctaatgtttt aactaaacct aaaaatctgt ttattccacg agtctctgaa tataaattaa
     2401 tattattcca atcctgtagg aatttttccc tatgatgaac atcaaaaatc tctgcagcaa
     2461 ttggcccgaa aaagagattg tgtttataag tcattgtatc catgacacaa ccatctgaat
     2521 caatacaaaa tatccaactg ttcatgtttt ttctcctagt tatgtttttg atttagcaaa
     2581 ttataatttt ctaatacttt ttctttagat aatggatata cgaaaaataa aatcaatcct
     2641 acaacaaaga aacaaactgc tggaatcaac gttgaagagt tatacagctt ttgtaaaact
     2701 tctccacttt gtacttttgc taacgaatca taccctatta tagaaagagt ccaacctccg
     2761 acaccgccag ctaaagcttg tccaattttt cgggcaaaag aatatactgc ataaatagtt
     2821 ccatcttctc tttggccaga tttcacttct tgatcatcga ttacatcagt aatattcgcc
     2881 caaatgactg tattaaaaat acctaacccc atatatccaa cagaagtaag aactaagaat
     2941 acatacatat tactaggctt tatcaaaaat aaaagtaaat atacaatagc agagaagaac
     3001 atccctactg acgccaattc ttttttaccg tattttttac tgaaaattaa acttacaggt
     3061 gaaacaagta ccaacatcaa aataggattg ataaagttca ttaaaactat tccctgtgca
     3121 ttattataat aatctggaaa tacataattg ttcatactca ttgttaaaag ctgagcaagc
     3181 aacaaaaaaa tagctgcagc tattatagaa agcaacgctc tatttttagc taatcctttt
     3241 aaagatttca acaatgaaac ttttaactgc gattcatcac ttttaacccg ttctacagtt
     3301 aatttataac atattacgta acaaataact gcacacactg aaaatactcc tgccacaatt
     3361 gtaaaagttg atccaccacg tacaatttga gctccagaac tatctcttgt ataaataaaa
     3421 attggtgtta acacgccaat gaacatacct gctaaggtag ctcctatact acgaaatgtt
     3481 gataacgctg tcctttcttc tgcaacagaa gtgatagctg atgccattga accgtaagga
     3541 atattaatag atgtataaaa aatacttccc catagtatat atgttacata catatatata
     3601 actttaatac tcatagaagc tgatgccatt gatgtttgat acattaaaaa acttgctaaa
     3661 gcgactggac cactcatccg taaaatccac ggtttaaatt tgccagtttt agatggtttt
     3721 gatccatcca caatacgccc cattgttatg tctgtaactg catcaataaa tcttgctact
     3781 aaaaataatg ttcctacggt agctccgctt atccccaata cttttgtata aaaaatcatt
     3841 aaaaaagaac tagcaaaaat gaaagtgaaa tcattcccaa aatctccaaa catatatcct
     3901 attttgtctc ttagaccaaa ttttctttcc atataatttg cgctccttcc tattgttaag
     3961 cgttttcatt ctgttgaact gccaacttac gtactatact ctgaagtata aagagttagt
     4021 atttattttt tacattttat agtattattt attttttctc ctaataattc tttatctcat
     4081 aaaacttatc tatatattta ctagctattc gagttacttc ttcaaaatca cctttttcaa
     4141 caggagccaa caaattccca cctacaccaa ctgcaacaac gcctgcttca aaccactctt
     4201 ctaagttatc tagacttact ccgccagtag gcattatatt tacatatggt aatggagctt
     4261 gatacgtttt tacaacagat ggaccaaaag cagaacctgg aaatagttta atgatttcta
     4321 ctccagcggt aagtgcctcc tttatctcag taattgtcat acaacctggt aaataaggaa
     4381 tttgatataa attacaaatc tctgctgttt gtcgatcaaa agatggactt acgatatatt
     4441 ctgctccagc cataattgca atacgagcag taaacgcatc taaaactgta ccggctccaa
     4501 ttattacatc tttttgatca gcatattttt tagcaatttc tttgattatt tcttcagcat
     4561 tcggaactgt aaaagttagt tcaatccctt taattccacc cgcagtaatt gcatttattg
     4621 ctttaatggc ctcatcttta ttttttccac gtacaaccgc aatgactcca gccgcttcta
     4681 acttacttaa aatttctact tttttcatat ctatctcctt cttttatgga tacatactct
     4741 agctagttgt actaacttac aataaataaa tttaagcttt tattttttgt attattttct
     4801 actttcgatt tcaaaaaaat taaaataatt atacgcatta cgataacaaa tattctccac
     4861 tatctgacta agatacattt catcatctgg aattcgacct gctttaaccc actcgcttag
     4921 taattgacat aatactcgac ggaaatactc atggcgagga tagcttagaa aacttcgaga
     4981 atcagtcaac atacctgtaa aattcgacaa caaactttgt tgcgccatcg tagtcaactg
     5041 ttgacgcatt ccttcagctg tatcattgaa ccaccaagca catcccaatt ggattttttg
     5101 aatcataccc ccttggaagc tttgcattgc tgttgctagt ggaagccaat cggttggatt
     5161 taatgaatat aaaattgttt ttggtaagca cccactcttt tcagcagtgt cgaacaatgc
     5221 tacaacattt tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga catctactcc
     5281 aatttgttcc tttaatatac ttgaattatt tcttaaagca tttaaatgca tttgcatcgt
     5341 ccaaccatat cttgtatact cagacatcaa atgtaattga atacctgtaa caaattgtgc
     5401 aatttctgat aaatggaaat ccgtttttcc tgctaaagct tttattaaca gattgtcgag
     5461 tattttttca tctgctattt cataaaaata agtattcaaa ccgtgatctg ctaatcgaca
     5521 gccaatttga tgaaaataat ccactcgttg gctcaatgcc tgcttcaacc cattaaagtt
     5581 tttgattggt atacccgctg cttgactaag ttttaaaata tagttcgaaa atcctgatga
     5641 tttgatactc cagaccttat ctggacggaa agtaggtaat accttgaaag aattatcctg
     5701 tttagaaagc tcagtatgat atcgcaagtc atctgtagga tcatccgttg tacaaatggc
     5761 tttcacatcc atcatcttca gcaattcttt tgggcgatag gaatccgttt ggattttttc
     5821 gttggcacgt cgccaaattt ctggtgcatt cttctcagaa attgtcaaat caatatcaaa
     5881 gtaacgttgt aattccaaat gagaccattc aaaaatagga ttacctggcg ccttctccac
     5941 tgtttttaca aaagctaaaa atttttcata atcattacct gcaccactga tcaactcttc
     6001 ttctatccca ttagctcgca tcaatcgcca tttataatga tctccagctc ctttatcatt
     6061 taaccatacc tgagtcaaat tcttgtattg cttattttct agcaaacatg ctgggtctaa
     6121 atgacaatga taatcgatga ttggttggtg ttctgcaaat ttatgaaaaa gtcttttagc
     6181 tatttcattc tctaataaaa agttcttatc taaaaacata tttaagcact cctttccttc
     6241 ccttagtttt tagaagagaa aatttttctt ctaaatagac tccattatat aattatttac
     6301 tcgccccctt ttcaagcgct tcccaaatac catttaaata aactgcacct agagctcggt
     6361 catataaccc ataaccaggt ctaccggttt ctccccaaat catccgacca tgatctggac
     6421 gaatatagcc attaaatcca ttatcgtaca ttgtttttaa tatcttatac atatctagag
     6481 aaccatattc tgatttatgc ggcgcttcat aaaaatcgag tccattatca cttaaatatt
     6541 tgatattacg aacatgtgca aaaggaatcc gatcccgttg acaaaattca gatagaattt
     6601 catagacatc attatctggt cgttctgcaa tacttccagt acataacgta ataccattag
     6661 aaggagaatc cactacatta cacaaccatt ctaaatcgtc acgattttta acaatacgag
     6721 gtaatccgaa aatagaatat ggtggatcat ctggatgaac tgccatttta atcccaactt
     6781 cttcacatgt cgggataatt gctttgagaa aatatgcaaa gttctctcgc aatttcgctt
     6841 catcaacttc tttataggct tctaataatt gcttgatttg tgataaccga tgaggttccc
     6901 aacccggcaa ttcaaaatta ttagtagaat tttctacttt tctaataatt tcctctggat
     6961 cagtaggaat attttttgaa atataggcta acgtagaaga cccatcatct aaatgataat
     7021 ccaaatctga ttttacccaa tcaaagactg gcataaaatt ataacagata acctcaatcc
     7081 caaattctgc caaattgcga atcgtctgtt tataattctc gatgtattga tctcgtgttg
     7141 gcaaaccaat tttgatatca tcatgaacat taacactctc aataactttc aaggtaaggc
     7201 cattggcttc gacttcatct tttaaagctt taattcgttc tctcggccag acctctccgg
     7261 ctggtatatc cattaatgtt ccaacaattc ctttcattgc tgggatctga cgaatttctt
     7321 gtagtgagat tttttcttca tttgccccat accatctaaa agtcatttcc atagttactt
     7381 tcctccaact tctgttatat atttattgat tgttttacga actgcttgag gacctactag
     7441 catttcgcaa aagatttttt caattttctt tcctatctca acttgatata aatctaaacc
     7501 aaatatttcc ttattttcta agatttcctg taccgcacaa tgaatctttg caggatcttg
     7561 ttcacctaat ttcaatactg agaatttttc ctgcaactct accaataaag gatcaggact
     7621 tggcacaaaa ttttttcctt gatcatcgac tgccaataaa taacgtaacc aagcagcaat
     7681 agcgatagga ataaatgtta gattttctgc atttccgtct ttttcgacat actttttaat
     7741 tgtttcacca tagcgaatcg ctactttttg agaagtatct gttgcaattc gttgcggtgt
     7801 atctggaata tagggattag gcaaacgttt attgattact tcatcaataa attgttttgg
     7861 atcaataacg cctggacttt caactactgg taagccttct acataaccta tccctttaat
     7921 taagccaacc aaatcatcat cttgcatttc tgcctgaatt gatttatacc ccaacaaaca
     7981 acctgttatt gctaatgccg tatggagcgg atttaaacat gtcgtaacct tcatgatatc
     8041 tgctttatca actgtttctc tatctgtcat aatcacacca gccgcttcca gtttaggtcg
     8101 accatttgga aaactatctt caatgactag ataatggact ttttcagtat tggtaaaagc
     8161 agcgatattg gtatttttcg cagtatgaat tatatctaag tcttcgaaac ccgccttcaa
     8221 taaatccatt ttgacacttt ccgaaggatt tggcgtaatt ctatcaatca tactccaagg
     8281 aaaagtcact ttttctttat cctttaaata agcaataaat ttttcagaaa cgtatccttt
     8341 tttttgccaa gcttctgcaa tcataaaaat gctatcttga aaacgttgac cattctgtga
     8401 gaaattatca gtagaaacca tcgcaatcgg taactctcca gcattaaacc tgctatacaa
     8461 caaggatgtc acaatactca tcgtatgttg aggattggta ggtccagatt ttatatctgc
     8521 ttccactacc gaaaaaaatt gtcctttaga atcttttaaa ctgtatcctt tttcagtaat
     8581 agtaaacgtt gtaaactgta atgtaggatc ttcaaaatat ttaaccactc tttgatagtc
     8641 cgttatattc tttggattgc aatataaact atcagctgtt actgctaaaa gtcttttttt
     8701 gagttcacca tcttcaccca taacaatctc caaaatatca ttgttgtagt catgataagc
     8761 tttttctact acttcttcat caaatgtctc acacacaact actccggatt ctaattcatt
     8821 ttgatctgct aagcattgag caatttctgc atgaaatcct cgataaagat ttcctccacc
     8881 aaaatgaagc cacactggat gtttctgacc ttcttgtttg accgcttcga cattaaaata
     8941 cggtagttga acttgcattt tgttcaatcc ttcgatagaa tcttgccagt ctttaatcaa
     9001 aaccatattt acccctccat actgatatgt tagtttccgt tacaattgta gtataaaatc
     9061 gaatgtaatc gctgtcaata agaaaaaact aaaaatattc ttttttctac catgtcagaa
     9121 ttgttgtgtc tttttttagt aaaagcacac caatatatat tttttagaac taacttaaat
     9181 taaagcttaa tttctctttg atcttatttg agtatttttt gtttttttaa tagctagtat
     9241 gttactttga aatttgatat tatataacga gcatagaact aaaatatcaa attcaaaaat
     9301 gaaattttga gagggattaa atatgacaat taaaactagt ctccaaaatc aagcttatca
     9361 atctattcgt cagcaaatta tctacgctga cttagaacct ggaaaaaaag tatcagaaaa
     9421 aaaattggaa gaaacgcttt ctatcggtcg tactccaata cgtgaagcat tgattcaact
     9481 ccgtcatcaa ggacttgttg atacaattcc tcaaagtaga aattatatct cattaatcga
     9541 cttagattta gcaaaaaatg ctcgttttgt cagagaacat ttagaacgac aaattatgct
     9601 agagtgttgt gccaagatga ataatcaaaa gaaacaagtt ttagaaacta taattgcaga
     9661 acaagaaaaa gctgttagtc aaaagaatgc tcgtagtttt tttcataatg ataatttatt
     9721 tcataaagct tgttttgaaa ttgcagaacg ggaagaagta tggcaatggt tggaagataa
     9781 taatacgcac ttggaacgat ttcgttggtt acgagtaaaa acggaaggat taaaatggga
     9841 aaaaattatg gaacaacatc atcaactatt taaagccttg accatgcaag accctgaaga
     9901 agctaacttc ttaacatcct tgcatcttca tttaatgctt gatgaacaaa atgttgtatt
     9961 gactactcac ccaaaatatt ttaaaaatat ataactgcta aatttctttt ttttaattta
    10021 tttacatttc tgattactac agaaaactaa c
//
LOCUS       DANJJH010000098         9707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000098 DANJJH010000000
VERSION     DANJJH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            278..787
                     /locus_tag="PWS53_002383"
     CDS             278..787
                     /locus_tag="PWS53_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0872580.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWS53_002384"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWS53_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0872581.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWS53_002385"
     CDS             2322..3302
                     /locus_tag="PWS53_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0872582.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWS53_002386"
     CDS             3424..4842
                     /locus_tag="PWS53_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL0872583.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWS53_002387"
     CDS             4913..6382
                     /locus_tag="PWS53_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0872584.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWS53_002388"
     CDS             6392..6859
                     /locus_tag="PWS53_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0872585.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWS53_002389"
     CDS             6895..7767
                     /locus_tag="PWS53_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0872586.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9707
                     /locus_tag="PWS53_002390"
     CDS             7848..>9707
                     /locus_tag="PWS53_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0872587.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYY"
BASE COUNT         3355 a         1417 c         1941 g         2994 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattat
//
LOCUS       DANJJH010000099         9615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000099 DANJJH010000000
VERSION     DANJJH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..626)
                     /locus_tag="PWS53_002391"
     CDS             complement(<1..626)
                     /locus_tag="PWS53_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0872588.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPR"
     gene            827..2041
                     /locus_tag="PWS53_002392"
                     /pseudo
     CDS             827..2041
                     /locus_tag="PWS53_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2220..2549)
                     /locus_tag="PWS53_002393"
     CDS             complement(2220..2549)
                     /locus_tag="PWS53_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0872589.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2539..2811)
                     /locus_tag="PWS53_002394"
     CDS             complement(2539..2811)
                     /locus_tag="PWS53_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0872590.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3262..4065)
                     /locus_tag="PWS53_002395"
     CDS             complement(3262..4065)
                     /locus_tag="PWS53_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0872591.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4043..5167)
                     /locus_tag="PWS53_002396"
     CDS             complement(4043..5167)
                     /locus_tag="PWS53_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872592.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5183..5998)
                     /locus_tag="PWS53_002397"
     CDS             complement(5183..5998)
                     /locus_tag="PWS53_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0872593.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5988..6860)
                     /locus_tag="PWS53_002398"
     CDS             complement(5988..6860)
                     /locus_tag="PWS53_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0872594.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6945..8234)
                     /locus_tag="PWS53_002399"
     CDS             complement(6945..8234)
                     /locus_tag="PWS53_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0872595.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8462..9106)
                     /locus_tag="PWS53_002400"
                     /pseudo
     CDS             complement(8462..9106)
                     /locus_tag="PWS53_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2971 a         2028 c         1557 g         3059 t
ORIGIN      
        1 tttctaggaa agtgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtgactgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaattgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaatatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaaggaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaagcaaaat caattctacc
      781 ttagatgttc ttatatcact ctatcagtcc tatttgacaa agagccaaga aaaagacgtt
      841 ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt
      901 ggtagatgga aacgggaaag caattatagt gaaaaatggg aagaaggaaa cgattgtccg
      961 ttttgaacaa tacaatcata tgcctttggt tacgcgccta aaaaagcaac gctatacctg
     1021 taaaaactgt cgtacccatt ggacggctca aagttatttt gtccgactca gccattcaat
     1081 cgcagatcat gttcgatata aaattgcttc tttactgact gaaaaagtat ctttgtcttt
     1141 tattgcgaaa agttgtcagg tatctttgac taccgttact cgtacattga aagagtttaa
     1201 aagctattta ccaaagcctt ctaagaagat tctccccaga gtattgatgg ttgatgaatt
     1261 tcgttcgcac gcttccatag aagataaaat gagctttatt tgcgcagatg gtgaaacagg
     1321 aaacttaatt gctgtcttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg
     1381 ctgcaccaat ccagaagaag tagaattctt agtcacagac atgaacgccg cttacttcca
     1441 gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt gatcggtttc atattgtcaa
     1501 acacatgaat caggcactca atgagttgcg tatccgtgaa atgaatgagc ttcgtaaagc
     1561 aggacagaaa agtcaggcag aaaaactgaa aaagaactgg cgctttctgc taaaaaatcg
     1621 tgcaagcatc aaccattatg agtacaaaac atggaaaagt ttccgagcac caaaataccc
     1681 atttctcact gaagcaatga tgattgatcg attgcttgag ttttctgcgc ctctaaagga
     1741 ggcgtatccc ttttttcatg aattagtcga agcctttcga gacaaagacc ctgacttatt
     1801 tttctcctta ttggcagaac ttcccgaaac gttagatgac ggctttcggg agaagcttca
     1861 aaaccttctg acctatgaag aaggtatcac cagcgcaatg atctatcctt attccaatgg
     1921 aaaaattgaa gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa
     1981 atcattcgag aacatgagaa ttagaatctt tttgatcaat caattaatca atgtaagata
     2041 acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg atttgatttt gcacaactca
     2101 tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa tgaggttcat tctgacccag
     2161 atttgatttt gtacaaccca tcagtcctta ttgacaaaga gcccttcttt ttagtattat
     2221 tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc ttggacttaa ttccccaatt
     2281 tttttccatt tgaccttttt gttgtttttg ggtaattcgt ttagagttcc tacgtcaatc
     2341 caagattctt tttttagtcc tgcctgtttc caatccctta tttcaacgta tttcagcttt
     2401 attttatcgg atttagtatg atattttgaa gtaattgaca gaaacgaaat tgtgtccagt
     2461 ccatttttta gaatatagac aggccggcgt ttaccgccct ctgcagctgt gaaagcaacg
     2521 tagacggttg ctatatcatt agtattcatc ttcattgaac cattcttcaa ttttttcgtc
     2581 agtgtctagt ttttggacag gcaatttttc cgctaatctt tgaatattat ggattcgttt
     2641 acttttttcc gacaattcaa tagaaaatgg gatttttcct tgctcaacaa tttgcttata
     2701 aaaaacggtg acggctgttt tggggctcat gcctagttca tcaataatgc tttctgcttc
     2761 atttttcaat ttttcgtcca tagaaatttg taacttagcc attttctcca cctctttctg
     2821 acaaacacat tatatcacgg aaagagttca aatggaatta atacaactac ttttgaactc
     2881 cttgaaatgc agtgtattta tctaaatata aggaaaggat atagtgctaa atggccttga
     2941 tagatagaaa agtttcaaaa tccgccacag cgataattga agcgaacact caacgacaca
     3001 cagaacagga aaagactcct aaaagaacca aattaactag gacgtgtctg aaaactccac
     3061 aaagatgtgg tatgctctgg taaaaaacga atggcacaac cacgaagacc gatatggttc
     3121 ttggaaaact ttctccaccc tttttgtaat tggagggata gtggtttatt gatagcgatt
     3181 ttccaagttc ttcaagtgga accagacatt gagcaccacg gctaaaaagt gaatgaagtc
     3241 tttattttta atcaataatt tttaatgatt accagtaatt ggttttttca tgcgccctat
     3301 ttctatttga tgtggagtta aataatgctg ataagtaaaa atttttccgt caattaaact
     3361 gccactagtg aatcctgttc tattggtaaa aagagaatag tctgggtatt catctacacc
     3421 aaatgataaa gattcagcca taaactgttt tttaccagca aattcagcac tgctaccttg
     3481 atggatatgt cctacaaaaa tacctaaaat atcactttcg gcaatcaatt cagcaaaacc
     3541 ttccggaact tcagttacaa tagatggatc ttcccacgac aatggatgat gaaccaataa
     3601 aatcgttccc ttttcagcag gtgtacccaa ccactcttgt aattgaatca tctgtcctat
     3661 cgatattttt ccttcgtgct gggtatcttg agcagaatcc aaggaaataa ttcgtaaccc
     3721 attttgattc gtacaatgga tatactctcc cgtttcattg ctttcaacac tcattccttc
     3781 aaagaaaaca ctgcgacgat catgattccc acgacaaaaa tagtaaggtg tggttgggaa
     3841 atattgatcc aacattttcc gtaagacttg ataatcagaa atcccccctt catgtactaa
     3901 atcacctgtt aatagaataa aatcgtacac tgttaacgag agttcttgta acaatttttc
     3961 caatgccaac tgtggatttt taattgttgg aaatagttca gcagtaaaca agttagtttg
     4021 atagttttct ctaaaatgga tatcagagag atgcaaaaag cgcatggcta ttccctcttt
     4081 cttcaataaa ataatcagga tatcctaaat tttcttgcgt ctgataatca aaaaaatgca
     4141 ttctttcagg ttctatgcgg agattgactg catcaccaat ctgccaagta ggtttttcat
     4201 taaggacaat catttctata ccatcgactt taacggtgta cgcataactt tgacctaata
     4261 gctcacgata cttcacttga ccgtgtaaat cgcttgtttc ttttgaaaag gttacgtgtt
     4321 ctggtcttat gcctaaatac aatgagccac taacctgaag aagttgtttt tgccaagctg
     4381 gattcaatgt taaaaattga ctggctacct ttaatgttcc ttgatcataa gtaactggca
     4441 taatattcat gcctggcgaa ccaataaaat ttgcagtaaa agtattttta ggtttcgtat
     4501 aaacattttt cggcgtatca atcatctgaa ttctaccttc attcaataaa acaatccggt
     4561 cagctaacgt catcgcttca atttgatcat gagtgacgta gacaaaagtt tgtttataac
     4621 gttgatgaat ttttaccaat tcttttcgtg catccaatcg taaacgcaca tccaaatttg
     4681 ataacggttc atctaataaa aaataattac tccgtttaac caccgcacga gccaatgcta
     4741 cacgctgccg ttgccctcct gacaaatctt taggaagtcg atcttggtat ggcgttaaac
     4801 ctaacatctt caaagcttct tccaaacggt gctgaatgtc agctgtctta attttattcg
     4861 cccttaatgc aaaaataata ttctccttta cagtcatgtg aggatataaa gcataatttt
     4921 gaaaaaccat tgcgatctct cgttctccac aaggtagctt atttgctagt tgtgtaccta
     4981 aaaaaagctc tccactcgta attttttcca aaccagcaat cattctcagc aaagtagatt
     5041 ttcctgaacc agatggtccc agtaacacaa ttcgttctcc agcggccaca gctaaatcaa
     5101 tttcttttaa aatggtatgt tctccatatt tttttccgat atttcttaat tcaagcgttt
     5161 ctcccatgat ttgtcctcca atttatttaa tacctgcaga aacaaaagtc ccgataatat
     5221 atttttgaaa aattgcatac aagactaacg gtacaaacat tgtaattaaa ctaatcgcca
     5281 ttgctacggt aaaatttgtc ccaccttcag aactaatata ggtctgcatc gccaacggca
     5341 aagtataatt atctactgtc cgtaaaatta ataccggcca aacatactca ttccaagagg
     5401 taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct agtaagagga aaaacaattt
     5461 tactcatgac ggttgtttgt ttgatgttat ccaatgccgc cacatctaga aggctcactg
     5521 gaatttcccg cattgtttgc gtcaataaaa aaatgcccat cgcatcagca aattgtggta
     5581 ttatcacacc ccaagtagta tcaattaaat taagttttgc aatcaacaaa taattaggaa
     5641 tcatcgcaac agtaaaagga atgaacatcg taccaatgat gataaagtac cagattttct
     5701 tccctttgat tcggcaataa gtaaaactat atgccgcaaa aaaagctacg cctaacttaa
     5761 caaccgttat aattgtagca ataaaaaaag tatttctcgt aattttgata attggaagat
     5821 tttcgaacaa atagcaaaaa ttttccaagg taaacggctg tggtatcaaa ctagtaatcg
     5881 tctgataggc atctttcaat ggcttaaaag aattggccat aataaacaat aatggataga
     5941 taataatgat tatgatgaac ataaataaga gatgtggcaa ccagcgttta attttcatag
     6001 taaaccccct tttccacaaa tttaaatatt acccctaaga aaacagcaaa tattaataaa
     6061 gttaaaactg aaattgctga agattctccg actttataca tttcaaaagc cttttgataa
     6121 gcataataga tcacgtttgc actaccatta ttcggtccac cacgagtgat gatcttaatc
     6181 ggagtaaaca catactgcag accttgaaca atggtcatca ccaataaata tagtcctgtc
     6241 gaactactca taggcaacac aattgtaaaa aaaatgcgcc agatgggtac acgatccaac
     6301 ttagctgctt cgatcatctc ttgatcaatt cccacaattg ctgcaaaaat gacaatgaaa
     6361 ttataaccaa aaactttcca agcagtaata cctgcaatca cagcaattac ccaaccttgt
     6421 aaattcatcc acataggcag tcttaaacca aaatttccca acaagacagc aattggtccc
     6481 gataccggat ttaatatcca tgtaaaaacc atccccgcca ctaccaaaga aatcacagct
     6541 ggaataaaaa ataatggttt atacaaattc tgcccttttt ctataactat tcctaataaa
     6601 aaggatgtaa tatatgggaa cacacagctc agcaagacaa atagaacggt ataggacaaa
     6661 gtattcttca acactttatg aaagactgga tcagtcagca cctttacata gttggcaaag
     6721 ccaacaaatt ctttagtggg ggccaccata ttccagtcaa aaaaactcaa atagatcgta
     6781 taaatcaatg gataaaaaac aaacacgccc aacaaaataa ttgctggcgc cagatacaaa
     6841 taacctacat agcgcttcat ttccgacagc ctcctcatcc ttatgtcaaa agcagacgac
     6901 cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct ttgcttattt gagtaactca
     6961 ttaatttcct tttgagtagc tttcatcgtt tcattgacgg aattacctga taaaatttta
     7021 tcccgcatat ccaacaattt ttgttcagct tctaatccag catctccagg aaatgaactc
     7081 caactgacca tcttatccat ctgatcaatt gctggtatca tcatctgatt ttcagctaag
     7141 aaagctttta atccattttg ggcgtctgca acatctttac gtggtggtaa ataaccagta
     7201 ccttctgtcc atttagctaa agattcaaca ctataaagat attttaagaa atcccaagtt
     7261 gcttcctttt tcttatccgt ttgtgatgta acagctaaca ttgcgccacc agcaggtagt
     7321 ttaacttttt tcccttccca acttggtgac tcaactgctt tagcagtaaa agtactattt
     7381 ttttgaatgt tgtccctttg agcaatagtg gtatagacca tcgcgacgtt accatcaata
     7441 aagctttgca caccttgttc ccacgtagtg tgtaatgccg tctggtcttt taccatcctt
     7501 tgatataatt cataagcagc gataccatct ttagaagcaa aggtagcttt gccatctttt
     7561 attacacttg ccccattact ttctaaaatt gcttgttgtg cccaattatc agcaggttct
     7621 tgaatataca cgccatactt tccagttttt tcttttatta tcttagaaaa agtttgaact
     7681 tcttcccaag tttttggacc attttcatcc aagcccgctg cttttaatat atcttgattt
     7741 aaatataaaa ccggattact aatggagtaa ggaataccca cttgtttacc atctttttca
     7801 gacagtgcca tgatattgtc taaaaaatga tctttaaaat aatcagcatt atccggatcc
     7861 acttcttcta ctaaagattg aggattagta tatttgaaat tttctgcaaa ataatcttta
     7921 aaagcccatc ccacttgtac tacgtccggt atttctttgg aggcaacaga agcttgtaaa
     7981 ttttgcatca agcctttata catatcagga ttatattttg ctacaacctt cactttgtct
     8041 tgagattgat tgtatttatc tactaattca ttcactgttt ttccacctag ggtatccgca
     8101 ttaacatgcc aatactctat cgttacacga ccctccgatt tttttgttgt cttggtattc
     8161 ccactaccac aagctgccaa agagatgaca cttaaaacag acacaacccc tataacaaga
     8221 tttttcttta acataacatt aacttcctcc taatttcttt atggccattt ttctaatcaa
     8281 gaaatattat gaactaccaa tgtaaatttc tagttaattt tctgtaaata attcgaaatt
     8341 tgtgcatttc ggggtacagg gtcactggac atatccatca agctaagaat aaagccccca
     8401 tatggtcatg tatcccatct ccaaatctct taactcctct gttttgatta taaaatagtt
     8461 tattctgtgc tcctttcttc cataaatagg cccatgcaca acgtgaggtt atattataaa
     8521 caccacagcg cgcaaggtac tcaatcgttt ctagttcatt gactgtccat acagaaaatg
     8581 tcaatcctag ctgctgcccc acttcacggg ccaacttagt taaccatcct tcatgaatat
     8641 tgacgatttg aacgctttga taggctaatt tctgtaaggt atcgatcaaa tctgcttcgg
     8701 taatatcccc aagttttttc ccttcgaact gattttcaat attataaaat atatgctttc
     8761 cccaaatcgc agcccaagat aattgtacat agccactaag aacaatttgt ggccagattt
     8821 gataggtttg tgccagcgaa aaaacggcca tttccaatcc ctcttctttt aaatcacagt
     8881 taatggtcat ggttgccttc tcattggcca ctgcttgaaa cacctgctca agggttaccg
     8941 aatctgggac aacttcttac ctgtcatggg ctagtattag cgtaccatct gtttgctgac
     9001 gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa gcgaagaaat gcccagctat
     9061 tctcctccgt attgtcacta ccagagtgtg cagtgatcat ggtcattttg acccctcctt
     9121 taattccgtg attttctccc acacgtccac tataccgatg aagcgttaat ttcttgttaa
     9181 tttcttgtaa attccctgcg ttttttcagt aaactagttg taaatcttgc atgctccttc
     9241 ctacgaactc atgaatcgtc gattttgccc ttctagctag tttcaccaat taagtagttc
     9301 tacattcccc gcttttaacc tactacacct ttttttgttt tcagctattc cttcttcttg
     9361 ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa ggtgttcaat ggcttattcc
     9421 cattgaatga ccgagactta ctatcagatt tctgctcgtt gaaccaagtc tttgatacag
     9481 gatttcttcc gtcatcttct cacaaatacc accttgcgtt tctctagcga ttggtcgatg
     9541 tgcaccctcc cagtggactg tcactacccc tttttgctat gcctagccca caaaaaaagc
     9601 tgtcctcaca gacag
//
LOCUS       DANJJH010000100         9612 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000100 DANJJH010000000
VERSION     DANJJH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(226..648)
                     /locus_tag="PWS53_002401"
     CDS             complement(226..648)
                     /locus_tag="PWS53_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0872596.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(638..2812)
                     /locus_tag="PWS53_002402"
     CDS             complement(638..2812)
                     /locus_tag="PWS53_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HDL0872597.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2828..5170)
                     /locus_tag="PWS53_002403"
     CDS             complement(2828..5170)
                     /locus_tag="PWS53_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872598.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5192..7735)
                     /locus_tag="PWS53_002404"
     CDS             complement(5192..7735)
                     /locus_tag="PWS53_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0872599.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7745..8143)
                     /locus_tag="PWS53_002405"
     CDS             complement(7745..8143)
                     /locus_tag="PWS53_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872600.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8224..8508)
                     /locus_tag="PWS53_002406"
     CDS             complement(8224..8508)
                     /locus_tag="PWS53_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872601.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8532..9530)
                     /locus_tag="PWS53_002407"
     CDS             complement(8532..9530)
                     /locus_tag="PWS53_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872602.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2753 a         2035 c         1492 g         3332 t
ORIGIN      
        1 gatttcaaac aaaatacgtt tattttttct attgatttta gctcaaaatc gattcaattc
       61 gagtgaaatt tgcttcaatt ttcaattggt ttttgttcaa attttgaaac atttttgctc
      121 aaaaaatgag tgaatttgtt tcaaaagaaa aacaaatacg atcgaaaaaa atttgaactc
      181 ataacaattt tttgtttttt tcaacgtatt ctctttcaat tttcactaaa aattaggaag
      241 aaattgattc aatgtctttt caaaaaagaa tgtttttctc tcaatttttt tgacgtttct
      301 atcatgaatc acgagaaaac ttgggacact tttaacatgg taacgttgta aaaaagtttg
      361 aagttcttct tgtcttggtt cttttcttgt ttcaattcga tatacattcc tagagaattt
      421 tttgtcgtat ctttccaaga aagattcaac ttcttggcat tttttacaat catttcttgc
      481 tagatacaca aaaaatgatt gattcgtttt ttgaagttct agtacttctt ctaaattact
      541 attgattagt tttccatttg attcttcgtt agtttttatc agtttaaaac aaccaaataa
      601 gaggaaacta acaataagta tcctaacaat aactttctta tttcgcatat tgtttctcca
      661 aatttggttt ccgaatatag acgactcgcg gtggttcctg gacgtcttca gctgttcgat
      721 acaccacaaa ttcgcctgtt ctcaatgctc gaatgcgatt cggattgaat ttaaagcggt
      781 ctacgtctcg tttcgtgcct ctttctcctg tccaatcaac ctttcccata tcagaataac
      841 ctgcttcttg tgtcgtcatt tccgttaatt caatgtcttc ttctgtcccc attaaatctg
      901 cccaataatc aatttctgtc gggtcatttg tcttcccaat aaagaacgta ttcacattcc
      961 caatcaactg ttttcgaagg acaggatcaa ttctatcaat gtccgcaagc gtttgagggg
     1021 catacgccgc atgcacccca gcgcctcgtc ccttattgac taagtctata aggttatcat
     1081 ttagataaga agctggttcg tcaaatatta ctaggacttt cgagcgattg gctgtttctc
     1141 ctgcttgttt atacacttct gacgcaaaga aattaatatc tcccacaagc atttgagcca
     1201 atgtccgaat aaactcacta taaatcaatc cgtttaacga gatataaaca atctttcctt
     1261 ctttaacaac ttcttgaata tctaattctc tttttcctcc agaagtatca aagagttcac
     1321 caagctcact atccaataac atattgatat tggtctgtaa attctcagaa gtcgcataca
     1381 aataaaacgg gttcttctta tgttcgtaac gaacaaacaa ccgtttaaac aacgtttgct
     1441 cgatttctgt catgagagaa atgttttctt ttaataatag ataatacgtt tctaagtcaa
     1501 cggttttacg atcatgttca acgattcgct cttcacttaa ctgttccaca tcggcaaaat
     1561 cacgttctgt tttgtccatc gcttcattct ttttcgtttt tttcttcgtg gttttcttct
     1621 tttctacgac cactttttct ttaaataaat ctggattttt caactgaata cgatccgcaa
     1681 acaaatgtaa gacgtttcgt ggcaataaat aatgttgtaa ccaaactaga tttctttcaa
     1741 ttttatggct cttttggaac tcgtctaaaa gctgaatcgt tacctgtaat aacgctttgg
     1801 ctgctgtcga ataatacacg ctttctgttt ctgctagatt ggttaattta tctcgaatgg
     1861 caatatcatt gccatttttt acaggattat aacgcaaatc gccaccgtcc acaaagactt
     1921 caatcgggcg attgtatgcc tctgccactt cttttaacgc ttgtaaagtc tctcctgctc
     1981 ctttcccatc gactaagata acggggatac gaaacttaca cgcatgttct gctagtaatt
     2041 ggatcaccgt cgtttttcct gaacctgtag tcgctggaat aatcacatgt tggttcaatt
     2101 cactaaagtt catcacataa gggagcccat actcatctgt tcctaataga atcactttgg
     2161 ctaatgaagc cagttgttta ttccgcaaag caacgtcttt tatgacaatt gctacctggt
     2221 acgcttgctg atactctgct gtatattttt gggactcctg aaatttattc ttccgacgtt
     2281 ccttgtattc tttactattt agcaaagctg tttgccgttc tttctttgaa acaacttttc
     2341 ttttttcaat aaaagacgca tacgcaatca aacaacttcc tgcacctgtc ccacctaaaa
     2401 ggagggcaaa aatcgtttgt ccattaaact gcaacgcatg agaaaacaag tttaatggaa
     2461 ataataagta acacgacaaa taccctctag gaagaacaaa aataaaacaa aatagattta
     2521 atagcgtaaa acctaacgca aaaataaaaa aaagagagct tcttcgtttt aagaagctct
     2581 catactcaaa gtcataaata tgtgcacgtg tctctttttt atctatcata aagacaatcg
     2641 ctaaaggaat actgcctaca ataaaaaatg gatatagaca aacacctaat aaagtgacga
     2701 taaaataaag ttgttgaaac cgactttttg gcgtttcttt ttcttgtttt cttttgcgat
     2761 cgacagaaaa catttgctca aaatgtgtcg gtcgttttgg tgcattcatc atactatcga
     2821 tttcctccta tcgttttcgt tgtttcattt ctcttcgata aatcggttgt ttttctcgtt
     2881 ttcgtttcat ttttgcttga gttgctcgaa actcactccg tgtcttcggt gcttgttttg
     2941 gtgattttgt ttgtttcatg gttgagtatg ctttagatac ggttgaaggc gctttggttt
     3001 gtgctgttac tcgattgact gctgacatat gttcccgaac cccctctgga cgtcgaacag
     3061 atttcggtgt ggtagaacct gttgctcgtg gtacattccc ttttcttagt ggtttttgta
     3121 ccgcatgttt ttgtcctgca actgtccggg ttttcatcga acttgttttt gttacgggag
     3181 taagtgcctc cttccgacga cgacttgttt gtccctgacg acgttttcga tcgttcaatt
     3241 ctgttctagg cgtatgcttc tggccataag acttgtgttt ttgctttgga ttcgctaatg
     3301 acaccaccat tgcagaaccc tgattgcgat acggactacg agacgcacct tttctacttc
     3361 tttctcttag actaggattg ccttgcaatt ctcgttgtaa taaatcggct gtttttcttg
     3421 cctgtttctc cattgctgtt cgttgcatac gttcttgact ttgctcttct gcttgtttcg
     3481 cttgatcatg caagtctctt gctttttgtg ccatacgttc catacgattt ggtgctttat
     3541 gacgaggttg ttgtgcggat tcacgccgaa aagcacttcg tgtggcttgt ttcgccgttt
     3601 gtggcatacg aacgtctgag ccacccccac gttttcgttg acgttcttgt ctcttccgtg
     3661 cgttttcttt ttctttttgt ttctgtgctt ttctcgcttc tttgcgttct ttcaaccgtt
     3721 gtttggcttc ccttctcaat tttgcttctg ccccaaatgg atttcccgca aaagctaacg
     3781 ctcctcgaac acccattctt ccagtaaata gactgcctaa aaagcctcgg aacaaataaa
     3841 ttcccaacgg cgcaaacaaa taaacaatga tcaagagaat tttttcccat ggatcgtctt
     3901 gtgtactact taattggaac ccgtaactaa gaaaagaaga aaaaaggata catgcaaaac
     3961 ctgtcattcc ttttaaaaac gtcaaaatac caaaaacttt ggcatagtta ccaaacacat
     4021 tcattgtgac aagaaacaat cctaaaaaaa gtaagaacgg caacatcggt actaacaata
     4081 gttgcatagt tccaataatc aatcgaaaca tacttaagac aaaataaaca acggtttgaa
     4141 tcagattcac gacaaaataa aacaaggcaa atgctgtatt tttccaacta ttgtaatatt
     4201 gtatgttctt gtttttcagt gtatctgctt catactctgt gactttgtca tgaattttct
     4261 cccctatttt tgtacctaaa tcattgtcca ataaaagatt gattcgatca tattcttttc
     4321 cagcatattt gactttcttc tcacgaattg tgtctacatt ggttgtgcca taatttaaca
     4381 acagatatgg ttcataaaca ttactataaa agacccgagc cgccatcaat ttgcctgccg
     4441 tttccacttt tttatccgct gttacctctt ctgtttcttt tgtttcataa tttgtttctt
     4501 cttcaggtgt taacactgga ttgaccttca cgaacgccgt ttccactgta gaatctaaag
     4561 aaaaaagaac atcaaaaaga ttcttatttg tcccagaatc ttttaatacg cttaacccag
     4621 taaaaataag gatcgtcata agaaagacac ggagtgcttg tttcattcga gcctctccag
     4681 catatttgat tgccatcata attgccgaaa aactgattcc aatagttcct gcaatactta
     4741 acatattgtc cgcaattttt tgtgttaagt tttgaatggg ttctcgaatg gcggaaataa
     4801 tatctaattc aaagagaaat tcaaccattg aagcattgaa ttctcctaac atttttacgc
     4861 cactccaaac aaaatttttc atcactcctg caacattgta cgatagcttt tttttaaggt
     4921 tcattgactc atagtcattg gtcatcaatt caaacgaatt ctttttgtaa tttttataca
     4981 attgatccac atcagtcgtt acttctttta cttctacttt atcgttggca ttctctcctt
     5041 cagaacgatc ggtttcagtg acacccgcac catcattcat ttgatcggca gaaacagtag
     5101 ccccttgtag acagaaaaaa agacccccta ataaaaggag cgtccaccat ttcagagatt
     5161 tttttcgcaa aaaaaccacc tcttttttgt tttatcgttg tttttcattt tctaacattg
     5221 cttgaatttt ttcatcttga ttttcagtag aagaaacagc caataaccaa ctatcaaaca
     5281 acacatcaat ggcaatcggt tgatttcttc ccaaatgatc ctggaataaa caggttcctg
     5341 tttgtaattg ttccatcatt tctaaattgg ctgaattcac ttccatatca aagaaggaca
     5401 atagtttctc tcgtgcttct ttttgtttcg gacgaaacgc aaatttgtaa gaaattaact
     5461 cttttttatc ttcttgatcg taatctttaa acgcttgcac aacaaggtaa atatccgtat
     5521 tattcgcacg tccttttcgc aagctatcat caatcaaata gcttccttgt gcggtatctt
     5581 catacccttt tgcctcgtca taaataatcg cagcgtcttc ttctttgttt gtagaaaaga
     5641 tatacgtaaa cttcataatg acttccataa ttgccattcc tgcaagttgt tcgctattta
     5701 atcgacccga atcaatctct ttttgtgtcg gaatgcgtat tccttgtgtc cctaacacgt
     5761 tgatttgtga atcaaaacgt atcggcgtac tgtgctcatt cccaatgaga atctttccta
     5821 aaccaacgct atgccctaaa atcaatttag ctagttcttt gtcacttttt cgaatttctt
     5881 cgataacttg acttaacgaa tgcttacctg gcatggtgta aactacatcg atggcgtctt
     5941 ggatcaccgt tttcttgtgg ctcgctgttt ctcgattggt atctacttct cctaacattc
     6001 ctaacacatt tttagcggtt tgaattgcct cttcttttgg taaaaacaat agaggatcaa
     6061 gcatccctcg acagctttca tctgaagata acgaaataaa attaattcgt tcatacatag
     6121 ccacaaactc tggtatgtgt ccgaattttt caagtgcctt taaaaagaac ttttgggttt
     6181 catttttcgg atcaatataa agaatttttt gacctaaaaa catcagccat aaaaaaatat
     6241 attttacaag gaccgatttt cctttacctg gtggaccaat aatcaacgta tttccattgg
     6301 tatgttccgc gccttcaata cttttctttg ttaaattggg ataataccat actagtttac
     6361 tagatagcgc taacgcttct ttcgcagatt taaacttttt tgcaataaca atccgtccta
     6421 aaggaaatcc tttcttattt cctacttctt tctttaagtc caagccataa tctgccacaa
     6481 aacctgtcgt agcgacctgc tcgtaactac gaaaacgttg cgtacttcca attaagcatt
     6541 gattaaacaa cgttagctga tccgctaccg atcgataaaa tttgaaatct gtagactcaa
     6601 tcgcaaattc aatatctttg actcgttctt ccaattcttc ttttgaagaa gcagatacaa
     6661 ccagcgtgac ggtagaataa cacaatcgtt tttctttgcg cttcagctta tcttttacat
     6721 ccattaaccg ttctgtacca aaatttaata catcgtctga atctaaaatg ccgtcaacgg
     6781 tctcatattc tttttcttgt tcataaatcc gtccagccat tttattaatt tttcgttcat
     6841 cagattttga atgctcaaat cgaagtcgaa tatgcgtatc aattggaaaa gataagctat
     6901 cctgaagatt ttgaataaag ctactcccat acatggctaa aggagcgtcc gtttgactta
     6961 aaaacgtcga ataatgcgtt tgattcaact gttcaatcgc aatgtatcct gcttcattcg
     7021 aaatgacgcc ctcgcaaatt tccattggcg taagctgtct ttcaggcaat ttttcttctg
     7081 atcgatggaa aaaataataa taaatttgtc caatctcttg ggcagataag cgctctatcg
     7141 gcttataatt ggacaattcc gtatagagct ccttttctaa tgtttcatac gttggtaaca
     7201 cactacttat aggaactcgt tgtcccgtaa tacgatagat aaacttttta aaaacatgct
     7261 taagcaattg aaaatattcg atcggatcag acactttcat ttctgtagtg aaacgcacaa
     7321 acagatacgt tcgatactcg tttaattgca cttcgtcttt taaaatcttt cctgcctgtt
     7381 caaagtacac tttcccaaga tctgcgcatt ctccctttac caaatctcga atggtttctt
     7441 caatgtgcgt atctaggtta aattgacgtg gaatattgat aaaatgatag tcatattcat
     7501 catgtccaag tatgccttcg ccatcttgaa gatagttctt gaaaaaatca atgtcattta
     7561 aaggaaatac ttgggaagaa agttgatagc ctacccacac atcatcattt ttggtcaaaa
     7621 caaaatttcg gataatggcg tctatcggaa attctaatcg aggaagcttc gtttttttct
     7681 ttttgaactg ccaaatcccg ataataaaga cgaccaaaag aaacaaaaac gccatttact
     7741 cgactcactc cttccatttg tgtagggaat acgtcacttt ttcatggaaa aacggcacat
     7801 acatttcatg ttcaaagcgt tgtttccgtt tctgataata ggacgttcgc ccaattaaat
     7861 actcgaaaaa gtgtttattg tcccatttca ttgaaaacaa tgtccaaacg agaacaccaa
     7921 tcccaaaaat cataatcaac caggactttc gaaacagtgt ctgcaagaaa ctaatttgat
     7981 tcacatacgc aatcaaaaat aggatcaaaa gaatgaagag aaatacccca cctatgacta
     8041 attgttccat gcgaatgcca ttagaaatcc agggaatttt ttgtccttta atcccgtaaa
     8101 ttttgtgagg gtgcttaaac tcctttgtat agttgtattc catgttcact ctacctcctt
     8161 ttacaaaaaa agaagcgacc agaagttcta atcttctgat cgcttttgca gtttgtcttt
     8221 ttcttatgat aattgtatcc agggcacaat catttgcgtt acccccacgt tctcttcact
     8281 atccttaata aagataatgt tctttagcca ttcaccaata tcaacggcat tcgataacaa
     8341 taagacgagc attatcaaaa taaagacgcc aataatggca aacgctgccc gcgcaaagcc
     8401 ttgggtcgca taagcaccaa ccattagaac aatcgcaacg attgccaata gccaaatgac
     8461 ttgtccagat acatcatcaa ataacgattt aagatcaaaa agtttcattt ccattctcct
     8521 ctaagttcat tttattcatt taacgtagta acaaaataac tatcgccact tgtttttact
     8581 tccattgaaa agtttgattg gaactcgttt cctttctcaa aatagaaacg atacttccct
     8641 tgcaaaaagt agtttccttc tttatttttt actgcatgat ccagttgtac attgatcaat
     8701 tcgccgcctt ctaacccttt ttcgttagaa atcaatgcca atttttcatc atttttgaca
     8761 tataactcaa aaaaccgttt cacaaatgct tccagcttga tttcttcgct ttcagtgacc
     8821 gcttgcccag aagaataaaa tcgattttcc tgatagttca cagaattggt attcttactc
     8881 tcagaaagat tcaaaaattg aacgccatca agtactttta aaacattgtt ttgataactg
     8941 actggaaaag aaagttgact gcttttccaa tgttctcctt ctgtaaaagt aacctcataa
     9001 aataggcgat acattgcttg tgtcaaatcg atttcttcta gtttaataaa gcgaacatct
     9061 ttcgctactc gattggtagc cgtcgttaca aagcctagat cagacgcttc caatccttgt
     9121 gctaaataat gattgacgga agcctcccat tcgtttcgtt tttctggcga ataagtaaat
     9181 aacctttcaa taaaatgttg tccctcgtac ttcaattgtt ccgtagaaga caaggtttgg
     9241 ttgacttcag ttaacaatgt ttctggattg acttgttttt tcactgctaa acgcgttaat
     9301 gtatccactc gtccaaagtt taggaggagc acgacaccca ttaacagaaa caaacaaaaa
     9361 aaagcgcctg aaaatacact catggcactt ttttgcgaaa attggatcat tcgatctgac
     9421 ggttctgttt tctttttttg gaacaaaaaa cgtttctttt gtttcttttc cttctgttgt
     9481 ttcggtggct tagtttggaa cgcttgtgga ttaaattttg cattactcat gagaaatgcc
     9541 ataccgttct aacaataatt tcggtatttt gtccacaatc actacttcgt ggacaaccac
     9601 acttgccccc tc
//
LOCUS       DANJJH010000101         9607 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000101 DANJJH010000000
VERSION     DANJJH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            23..646
                     /locus_tag="PWS53_002408"
     CDS             23..646
                     /locus_tag="PWS53_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872603.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PWS53_002409"
     CDS             1096..2145
                     /locus_tag="PWS53_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0872604.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PWS53_002410"
     CDS             2142..3023
                     /locus_tag="PWS53_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872605.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PWS53_002411"
     CDS             3026..3796
                     /locus_tag="PWS53_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0872606.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PWS53_002412"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PWS53_002412"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL0872607.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PWS53_002413"
     CDS             5107..6270
                     /locus_tag="PWS53_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0872608.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PWS53_002414"
     CDS             6387..6797
                     /locus_tag="PWS53_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0872609.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="PWS53_002415"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="PWS53_002415"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0872610.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..>9607
                     /locus_tag="PWS53_002416"
     CDS             9370..>9607
                     /locus_tag="PWS53_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872611.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIII"
BASE COUNT         3333 a         1632 c         2074 g         2568 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attattt
//
LOCUS       DANJJH010000102         9236 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000102 DANJJH010000000
VERSION     DANJJH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..635)
                     /locus_tag="PWS53_002417"
     CDS             complement(<1..635)
                     /locus_tag="PWS53_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0872612.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETF"
     gene            769..1056
                     /locus_tag="PWS53_002418"
     CDS             769..1056
                     /locus_tag="PWS53_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872613.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1472..3010
                     /locus_tag="PWS53_002419"
     CDS             1472..3010
                     /locus_tag="PWS53_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL0872614.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3007..3972
                     /locus_tag="PWS53_002420"
     CDS             3007..3972
                     /locus_tag="PWS53_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872615.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3959..5281
                     /locus_tag="PWS53_002421"
     CDS             3959..5281
                     /locus_tag="PWS53_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0872616.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5344..5448
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5522..6265
                     /locus_tag="PWS53_002422"
     CDS             5522..6265
                     /locus_tag="PWS53_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872617.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6280..7272
                     /locus_tag="PWS53_002423"
     CDS             6280..7272
                     /locus_tag="PWS53_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0872618.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7284..8021
                     /locus_tag="PWS53_002424"
     CDS             7284..8021
                     /locus_tag="PWS53_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872619.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8011..8733
                     /locus_tag="PWS53_002425"
     CDS             8011..8733
                     /locus_tag="PWS53_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0872620.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8817..>9236)
                     /locus_tag="PWS53_002426"
     CDS             complement(8817..>9236)
                     /locus_tag="PWS53_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL0872621.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3019 a         1597 c         1953 g         2667 t
ORIGIN      
        1 tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc aatttccaga
       61 tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt atacagaaaa
      121 tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat caagaaaaga
      181 cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat atctgttccg
      241 cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag aatacctgtc
      301 atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct ctcccataaa
      361 tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc gtgttttcca
      421 aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt gacagaagga
      481 gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc atctgccagc
      541 ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc taaaatgatt
      601 ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc ttttgaaaat
      661 tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa aatgaaaaaa
      721 aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat gcgagcagca
      781 gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg agtttttttg
      841 gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc aaatcaattt
      901 atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt tctttttttg
      961 atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat tcctctttat
     1021 ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa acaagacgtt
     1081 tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg atacagtata
     1141 atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac tttattggct
     1201 gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga ttccaagtga
     1261 caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa aaagttgatc
     1321 cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt tttctttttt
     1381 ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa agaaatgttt
     1441 atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa cattaagtga
     1501 aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc tcatttcttt
     1561 gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc ataaaaaaat
     1621 ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac ttgccttatt
     1681 aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg cacttgttat
     1741 ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa ttgacccgtc
     1801 tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc tgaaagtaga
     1861 gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag ttttaatcat
     1921 cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat ttattttgtt
     1981 aggtattgac cgaaatacac ctgttttgac attgattggt gcgatcagtt ctgccttgct
     2041 tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc gatattccat
     2101 cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc agtctgcctt
     2161 gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg aaatcctgat
     2221 ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat taaaaccgaa
     2281 ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg atatctatcc
     2341 tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa tgaaaagtgc
     2401 tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata agcaattcga
     2461 tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag tcaagagatc
     2521 ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct ctgatcagat
     2581 caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag gaatccaaga
     2641 agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc gttaccaagc
     2701 aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc agctcaagga
     2761 gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc aaggcgcacc
     2821 gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac tgaataaatt
     2881 aagcggaaaa ataacggagg atcaaatgat tgatatgaat taccaagtca atgtcgaaaa
     2941 gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag taaaggagga
     3001 agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga tggaaagtat
     3061 gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt ggttggtcct
     3121 tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa accatcagca
     3181 ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa aatgcggtgg
     3241 aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt caagcaaaat
     3301 attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga ccgtgtagat
     3361 gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat gcctagagag
     3421 ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc aagcccagat
     3481 gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa ctcattacaa
     3541 gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt tacgcacaat
     3601 atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg agaaattatc
     3661 caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt tcggcatttc
     3721 tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt caatggatat
     3781 ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac gcctatgaaa
     3841 gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa gcaaagaagt
     3901 attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga ggaaggaaat
     3961 gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg ctgcttatgg
     4021 attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg gcgggacttg
     4081 tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg cacgtaatcg
     4141 ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact gggaagaact
     4201 tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa aacaattagc
     4261 agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt catctattga
     4321 agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc aaagacctac
     4381 gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt tatcattgcc
     4441 tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg agctagctac
     4501 gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac ggccattaaa
     4561 agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat ctggcattca
     4621 gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct atcgtttagt
     4681 cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga gacagccgaa
     4741 tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg gaattgcagt
     4801 caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata ttgtttcaag
     4861 gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag ctaagcggat
     4921 aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg tctacgccag
     4981 tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag atcaagtggt
     5041 agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag ttgctaaggc
     5101 tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta gcgagcaagc
     5161 agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta aaaaagagtt
     5221 ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt tattgaaata
     5281 atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac tttaaaaaat
     5341 gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa cttagaccca
     5401 ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg aagcgggtta
     5461 ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag gaggtaacaa
     5521 aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta tttggcagat
     5581 tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg ctgtattttc
     5641 tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat taaatgaagc
     5701 agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg caatggatta
     5761 ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa tgttgccaat
     5821 catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc caaaagtgat
     5881 ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata cattgaggaa
     5941 agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag ggaagattta
     6001 tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa aagtggctgc
     6061 tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag caggcttagg
     6121 gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg ctgcgatcat
     6181 ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga aaaaagggta
     6241 ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt atggctttta
     6301 ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag tagcaaacaa
     6361 gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa tactaatcat
     6421 actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt aaatgtccaa
     6481 atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga caaagcacaa
     6541 tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca gaatatacca
     6601 gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac cctatcagac
     6661 agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg gcaaagtcca
     6721 agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt ttcaaaactg
     6781 acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc agatatccaa
     6841 tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga actaaactat
     6901 atcccattga aagaattaga tgaacggtta gattactata ctccggtaat tattacgaat
     6961 gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc cacgaaaaaa
     7021 ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca aaaatatgca
     7081 aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa aaattatacg
     7141 gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta tacagctttt
     7201 atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt cactaatcag
     7261 tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat gtagtcattg
     7321 aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc tttacatcga
     7381 ttgtcgcgcc aagcggagcc ggtaagtcaa cattgctgaa aactttaaca ggagtccagc
     7441 ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc catacagcat
     7501 ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat caaaacgtta
     7561 cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg gattatctgg
     7621 atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct ggcgggatga
     7681 agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg cttcttgatg
     7741 aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg atgttgaagc
     7801 tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa gaagcaattt
     7861 atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt gctgagattc
     7921 aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag ttgaagcaaa
     7981 cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg tcatttagat
     8041 gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat catcaattgt
     8101 gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac tcaagcctta
     8161 agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt tgagcctttt
     8221 ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac gaatgtaccg
     8281 atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat aaatgagaaa
     8341 cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat ccaagcttac
     8401 cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga ctatattgat
     8461 ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt ctggcaacta
     8521 gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca tgcagcagag
     8581 tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttga agaacagtta
     8641 aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa tacttatcaa
     8701 aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca actagtgtcg
     8761 cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa tgaatcttag
     8821 taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata acgtgaagta
     8881 acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt taagtaagaa
     8941 gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga accgcttgat
     9001 tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc agcagtttct
     9061 gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc cgtttgaacc
     9121 gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc tgcttgaggt
     9181 tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc tgagta
//
LOCUS       DANJJH010000103         8919 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000103 DANJJH010000000
VERSION     DANJJH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="PWS53_002427"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="PWS53_002427"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0872622.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWS53_002428"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="PWS53_002428"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL0872623.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4444)
                     /locus_tag="PWS53_002429"
     CDS             complement(3191..4444)
                     /locus_tag="PWS53_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0872624.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="PWS53_002430"
     CDS             complement(4450..5373)
                     /locus_tag="PWS53_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0872625.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="PWS53_002431"
     CDS             complement(5594..8086)
                     /locus_tag="PWS53_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0872626.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="PWS53_002432"
     CDS             complement(8093..8560)
                     /locus_tag="PWS53_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL0872627.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="PWS53_002433"
     tRNA            complement(8696..8769)
                     /locus_tag="PWS53_002433"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="PWS53_002434"
     tRNA            complement(8827..8900)
                     /locus_tag="PWS53_002434"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DANJJH010000104         8494 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000104 DANJJH010000000
VERSION     DANJJH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..173)
                     /locus_tag="PWS53_002435"
     CDS             complement(<1..173)
                     /locus_tag="PWS53_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872628.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWS53_002436"
     CDS             complement(593..1714)
                     /locus_tag="PWS53_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL0872629.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWS53_002437"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWS53_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL0872630.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWS53_002438"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWS53_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL0872631.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWS53_002439"
     CDS             complement(4304..5215)
                     /locus_tag="PWS53_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872632.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWS53_002440"
     CDS             complement(5232..6239)
                     /locus_tag="PWS53_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0872633.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWS53_002441"
     CDS             complement(6236..8365)
                     /locus_tag="PWS53_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0872634.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="PWS53_002442"
     CDS             complement(8370..>8494)
                     /locus_tag="PWS53_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0872635.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DANJJH010000105         8348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000105 DANJJH010000000
VERSION     DANJJH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            201..680
                     /locus_tag="PWS53_002443"
     CDS             201..680
                     /locus_tag="PWS53_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872636.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="PWS53_002444"
     CDS             713..2941
                     /locus_tag="PWS53_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0872637.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="PWS53_002445"
     CDS             3070..4713
                     /locus_tag="PWS53_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0872638.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="PWS53_002446"
     CDS             complement(4786..5331)
                     /locus_tag="PWS53_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL0872639.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="PWS53_002447"
     CDS             complement(5354..5839)
                     /locus_tag="PWS53_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0872640.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="PWS53_002448"
     CDS             complement(5829..6641)
                     /locus_tag="PWS53_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL0872641.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="PWS53_002449"
     CDS             complement(6780..8312)
                     /locus_tag="PWS53_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL0872642.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2815 a         1590 c         1643 g         2300 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaa
//
LOCUS       DANJJH010000106         8191 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000106 DANJJH010000000
VERSION     DANJJH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            19..549
                     /gene="lepB"
                     /locus_tag="PWS53_002450"
     CDS             19..549
                     /gene="lepB"
                     /locus_tag="PWS53_002450"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0872643.1"
                     /translation="MNRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSMENVLKQGD
                     MVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKVVKEPYLT
                     KNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTINKTDILG
                     KARFVYYPLDEIKWIQ"
     gene            complement(632..1132)
                     /locus_tag="PWS53_002451"
     CDS             complement(632..1132)
                     /locus_tag="PWS53_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0872644.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1411..1809)
                     /locus_tag="PWS53_002452"
     CDS             complement(1411..1809)
                     /locus_tag="PWS53_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0872645.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2069..2929
                     /gene="ylqF"
                     /locus_tag="PWS53_002453"
     CDS             2069..2929
                     /gene="ylqF"
                     /locus_tag="PWS53_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL0872646.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2922..3689
                     /locus_tag="PWS53_002454"
     CDS             2922..3689
                     /locus_tag="PWS53_002454"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL0872647.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3745..4602
                     /gene="dprA"
                     /locus_tag="PWS53_002455"
     CDS             3745..4602
                     /gene="dprA"
                     /locus_tag="PWS53_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL0872648.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4724..6802
                     /gene="topA"
                     /locus_tag="PWS53_002456"
     CDS             4724..6802
                     /gene="topA"
                     /locus_tag="PWS53_002456"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL0872649.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6771..8147
                     /gene="trmFO"
                     /locus_tag="PWS53_002457"
     CDS             6771..8147
                     /gene="trmFO"
                     /locus_tag="PWS53_002457"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL0872650.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2856 a         1445 c         1720 g         2170 t
ORIGIN      
        1 acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct
       61 gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat
      121 tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc
      181 agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta
      241 atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa
      301 gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat
      361 acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga agataattat
      421 tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat
      481 aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg
      541 atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc
      601 caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag
      661 cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat
      721 tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca
      781 gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg
      841 aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag
      901 ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt
      961 tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca
     1021 tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga
     1081 tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca
     1141 atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata
     1201 ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg
     1261 gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt
     1321 gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt
     1381 gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt
     1441 atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc
     1501 tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc
     1561 cgcgttttca acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc
     1621 tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc
     1681 atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag
     1741 cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat
     1801 actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa
     1861 cgctgtcaaa aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa
     1921 agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag atggaatttc
     1981 tttataataa aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata
     2041 ttccggtatg aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc
     2101 aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt
     2161 agacgcacga ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa
     2221 acctcgcctt gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg
     2281 gcaagaatat tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa
     2341 aggaattaaa ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga
     2401 tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt
     2461 tgggaaatct acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa
     2521 gcctggcgta acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga
     2581 cacaccaggg attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct
     2641 gacaggcgcg atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga
     2701 cttttttgcc cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca
     2761 agaagaaaaa ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga
     2821 agactatgat cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg
     2881 atacacactg gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa
     2941 gtatcaaaga acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa
     3001 gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa
     3061 aggaagcatt aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg
     3121 cacaaggtca ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac
     3181 ctgtcgtggc agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact
     3241 ccaaacaatt atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc
     3301 tggcaatcgg gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag
     3361 caagtaaatt agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt
     3421 tgatcgatgc gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg
     3481 cacgatctgt gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga
     3541 tgaaagaata tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa
     3601 caaaggaaca cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt
     3661 ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa
     3721 agagagacga aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct
     3781 tatacaaaag gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat
     3841 ccttataaag aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg
     3901 gagctgtttc gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa
     3961 cagtcgttta tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat
     4021 ccgccaattg tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc
     4081 gttgttggtg gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa
     4141 ccagtgatag aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac
     4201 ggtcaccaag cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata
     4261 gagcaagtct atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt
     4321 cttttgagtg aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat
     4381 cggattatcg cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc
     4441 tctctgataa cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga
     4501 aatattttaa cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc
     4561 acggtttcgg gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg
     4621 cttttcttga caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct
     4681 tttaatagta ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt
     4741 agtgattgta gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta
     4801 taaagtaatg gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga
     4861 ttttgaaaac aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa
     4921 aagcctgaaa gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag
     4981 agaaggagaa gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa
     5041 aaatcgggtg gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc
     5101 aagaacgatc gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat
     5161 cgttggttat tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg
     5221 acgtgtccaa tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt
     5281 tgttccagaa gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa
     5341 agcaaatttc tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa
     5401 agaagtcact gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga
     5461 gcgcaaaaga aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg
     5521 taagttaaac tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat
     5581 cactttgggt aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg
     5641 gattgccgat acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga
     5701 attttctaga catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga
     5761 agctgtgcgt ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa
     5821 agatcaattg aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc
     5881 agctatttta gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa
     5941 cggttcaaaa atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg
     6001 caaagaagag aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt
     6061 tgatattgaa ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt
     6121 gattcgtgca ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga
     6181 tacgatccaa agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact
     6241 aggagaaatt gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt
     6301 cacagcagaa atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa
     6361 agtggttgat cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat
     6421 cgaaaaaatt caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc
     6481 gatggtgata aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg
     6541 tcggaatacg aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg
     6601 acaagtcatt gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc
     6661 agaatgtgaa ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg
     6721 atatctggtc gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta
     6781 cgaagaagac atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg
     6841 ttggagcagg gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg
     6901 tccgtttata tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg
     6961 ctgaactagt atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat
     7021 tgaaagaaga aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag
     7081 tgccagctgg tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa
     7141 aagtcaaaga gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag
     7201 gaatcaccgt agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag
     7261 cattcaatgg ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag
     7321 ctacgatcga tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt
     7381 acttgaactg tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg
     7441 ccgaagtggc agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa
     7501 ttgaagtaat ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg
     7561 gattggaaga tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca
     7621 atgctgcagc ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc
     7681 aaaaacgtgt tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg
     7741 tgatgcaccg aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa
     7801 cgaaacgcga agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa
     7861 gtgcagcgag cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac
     7921 cagtcgagtt tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg
     7981 aaggaaaaca tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc
     8041 gcattcgaga caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg
     8101 aggcaataaa aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga
     8161 agagggtgag acggatagtc tcgctctttt t
//
LOCUS       DANJJH010000107         8134 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000107 DANJJH010000000
VERSION     DANJJH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(131..313)
                     /locus_tag="PWS53_002458"
                     /pseudo
     CDS             complement(131..313)
                     /locus_tag="PWS53_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            300..497
                     /locus_tag="PWS53_002459"
     CDS             300..497
                     /locus_tag="PWS53_002459"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872651.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(540..1115)
                     /locus_tag="PWS53_002460"
     CDS             complement(540..1115)
                     /locus_tag="PWS53_002460"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0872652.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1270..1796)
                     /locus_tag="PWS53_002461"
                     /pseudo
     CDS             complement(1270..1796)
                     /locus_tag="PWS53_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2253..3764
                     /gene="gadC"
                     /locus_tag="PWS53_002462"
     CDS             2253..3764
                     /gene="gadC"
                     /locus_tag="PWS53_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL0872653.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3777..5177
                     /locus_tag="PWS53_002463"
     CDS             3777..5177
                     /locus_tag="PWS53_002463"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL0872654.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5342..6782)
                     /locus_tag="PWS53_002464"
                     /pseudo
     CDS             complement(5342..6782)
                     /locus_tag="PWS53_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7175..7768
                     /locus_tag="PWS53_002465"
                     /pseudo
     CDS             7175..7768
                     /locus_tag="PWS53_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
     gene            complement(7752..>8134)
                     /locus_tag="PWS53_002466"
     CDS             complement(7752..>8134)
                     /locus_tag="PWS53_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0872655.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2543 a         1395 c         1481 g         2715 t
ORIGIN      
        1 ttttcaataa aaatatatta caataataat cgtaatagct aatgaaacaa tttctcaatc
       61 tttttaccac tccaaacagg gagcgtcaat aattttgtgt aaataactcg tccttctgca
      121 aaataatggg ttactcagta aacattgaag ctaatgtatc cgtcacttgt tggaagcctt
      181 tatggcttct atttaagaat ttttgattgt atgtatcaaa gatggatact aggaaacgct
      241 ctagtgattc ttcattttga aactgctctt ttctgcggct gtatttcttg atttgtttat
      301 tgaaagattc gattgtagaa ccagaaggaa tggagaacac ttttgaactt tacaacaaaa
      361 ctactgttga taaattaatt ccaattttag ttccaactga tttaaatggt catgatgttt
      421 tacttcgttt taacggattt ttaggtgagg attatagata cctaaaaatt tctgatcctc
      481 aacctttaac agaataataa aaaacgtgta atcaattatt tgattacacg ttttttattt
      541 caatcttcgt tgtagtagtg gttgcgacat accgaaaggt aatcttcatt acctcctact
      601 acaatctgtt cacctgaata aactggaata ccatcaacaa tacgagcatt cattacagcc
      661 tttttattgc aaaaatggca aatagtttta atttcctcaa ttttatcagc aaatgtcaat
      721 aatgcttcgg atcccttgaa gagattattt tgaaaatcat tcttcaatcc atagcaaatt
      781 actggtactt cctcattcat tacgataagt gatagatcaa taatattctc tctacttaaa
      841 aattgagact catcgaccaa aatacaatca acttttccgt actgatcaat aatctccact
      901 aattctccgt acacatccat ttcagaagta attgcaatag ctggtttcga aacacctata
      961 cgactctcaa cttttccaaa tcctgatcga gtatctattg cgcttgtcat tatgataact
     1021 ctcttccctt gttcttcata attatgagcc actttcagta gctcaatact ctttccacta
     1081 ttcattgttc catatctgta aaataattgt gccattaaat ttattccctt cttttacaat
     1141 tcaattattc cttaattcca tctgctttta tgcagctgct cgtttattcc ttgatatgtc
     1201 tataacatct actatcccta aaaccaattt ataagccatt agagatggta ttcctgaaca
     1261 tctctcatat tgttttttga gtggtatatc atctgaaaaa ctgaaggaat catgaaaata
     1321 gcgacatact tatcatttag agttctaact ttaaatgcat ttacttcttc agtcagcact
     1381 ttagtcatgt tggacatggt ttggggcgtg tagtgatgcc catacatttt ctcaatcaag
     1441 tctgcgattt ccgacatggt aatacctttt cggaagaggt gaatgacggt ctcctctaat
     1501 gtgtcattcg tccgtctata agcaggaaca gtctgttgct tgaactcgcc attgcggtcg
     1561 cgcggaatct ggagatgaag ttccccatac tcggtcttga ccgtacggtc ataggagccg
     1621 ttacgagaat tacccgtgtt aaaaccaatg cgatcgtact tttcgtaatc gaggaaagcc
     1681 gttaactccg ttttgagaag cgtattgaca gctttttcca aatggacacg gaataatcca
     1741 ttcaaatcgc ctttgttcgc tagagtcttt aggatttctg tagtaaaatc gttcatatgg
     1801 aagtcctctt ttctgtgaat gtgttgtggt aactctattc tacagaaggg acttcctttt
     1861 ttctatttac acaaaatatt ttacactctc aacaaaagta tcttcgatta atacttgctc
     1921 aagttatgtg cagctacgaa aaagtaggta ccgaggtact ttctttttcg cagctttttt
     1981 tggacttatg agaagtacat tcgctggaag aatacgatat aaaacgagct ttttcactaa
     2041 agatattaat agagtgaaag aaacaagctt ttgttgtcag ttttttcact tttctaaaaa
     2101 tgaaaataaa aaattctgga agtggagaag aattttgctt gctatacttt gtcttgtaga
     2161 aaagaggtgg acaaaataat gcaagctcaa ataagcgatt ttccataacc ttggtaggaa
     2221 tgacaaatta aataggaaag ggaagtaata atatgaataa aaaaaagtta tctctattcg
     2281 gtttttttgc cctgacagct tccatggttt tgactgttta tgagtatccg acctttgcaa
     2341 catcacaatt acatttggtt ttctttcttc ttttgggagg tattttatgg tttctaccag
     2401 ttgctttatg tgctgcagaa atggcaacag ttgaaggatg gaaagatggt gggattttca
     2461 gttgggttag tcaaacttta ggagaacgtt ttggttttgc agctattttt ttccaatggt
     2521 ttcaaattac agtaggtttt attacaatga tctacttcat actaggagcg ctgtcgtatg
     2581 taattaattt tccagagctt aatacagatc catttatcaa gtttattggt gtattagtca
     2641 ttttctgggt actgactttt tcacagttgg gcgggaccaa tcgtaccgct aaaattgcta
     2701 aagcaggatt tgtcattgga attgttattc cttcgatcat tctctttggt ttagcagcag
     2761 cctactttat cggcggtaat ccgattcaaa tggctattaa tgcgaaagct tttattcctg
     2821 acttttctca gctttcaact ttagtagtat ttgtatcttt tatattggct tatatgggcg
     2881 tagaagcttc agcatcacat attaatgagc tggaaaatcc ccaaagaaac tacccattgg
     2941 caatgttttt actagtgatt ttggccatcg ccttagatgc aatcggcggt ttctcagtag
     3001 cagcggttat tccacaacat gacttgtctc tcagtgctgg tgtaatccaa acatttgaaa
     3061 cgcttatttt acattttaac agtcatttac attggttggt aaaagtgatt gctctcatga
     3121 ttgcctgtgg cgttatgggt gaagttagtt cttgggtagt tggtccatca cgagggatgt
     3181 ttgcagctgc tcagcgaggt ctgttaccag ttttcttccg gaaaactaat aaacaagacg
     3241 ttccagttcc gttgattatg atacaaggaa ttatcgttac tatttgggca gcagtcttaa
     3301 cttttggtgg aggaggcaac aatttatcat tcttagtagc aatctcctta actgttgtca
     3361 tttatctcgt aggatattta ctatttttcc taggttactt tgtattagtt tttaagaaaa
     3421 aagacttaaa acgaacttac aatgtaccag gaaaaactgt tggcaaaacg attatcggtg
     3481 gaattggttt tattttatca gtgttcgcat tgcttatctc ttttgtacca ccagcttcaa
     3541 ttgcagctag tgagcaccga acataccaaa tcatactact tattagcttt gtagtgactg
     3601 ttcttttacc ttttatcatt tatgaagtac atgataaaag tgctcacaca actattgagg
     3661 aacctactca cgtgaaagct gaagacgtta atcccgctgt ttatccagct gctagaggag
     3721 agcaccacgt tacaaaaaat gatgaacata ttttaaaaca ttaggaggaa ttcattatgt
     3781 tatacggaaa agataatcaa gaagaaaaaa actatttgga accaattttt ggctctgcaa
     3841 gtgaggatgt tgacttgcct aaatataagt taaacaaaga atccattgaa ccacgaattg
     3901 cttatcaatt agtacaagac gagatgttgg atgaaggaaa tgcgcgatta aacttagcta
     3961 ctttttgtca aacgtatatg gaacctgaag cagtgaaatt gatgacccaa acgttagaaa
     4021 aaaatgcaat tgataaatca gaatacccac gaacaacgga aattgaaaac cgctgtgtaa
     4081 atatgattgc tgatttatgg catgctccaa ataatgaaaa attcatggga acttcaacga
     4141 tcggctcttc agaagcctgc atgctgggtg gtatggccat gaaatttgct tggcgtaaac
     4201 gtgctgaaaa attaggtctt gatattcaag caaaaaaacc taacctggtg atctcttctg
     4261 gttaccaagt ttgttgggaa aaattctgtg tatattggga tgtggaactg agagaagtcc
     4321 caatggatga aaaacatatg tcaattaatc tagatactgt catggattat gtggatgagt
     4381 acacaattgg tattgtaggt attatgggta ttacttacac tggtcgttat gatgatatca
     4441 agggtctgaa tgatttagtt gaagctcaca ataaacaaac tgactataaa gtatacattc
     4501 atgttgacgc tgcatcgggt ggcttttatg caccatttac tgaacctgat ctagtttggg
     4561 attttcaatt gaaaaatgtt atctcaatta attcttcagg tcacaaatat ggtttggtat
     4621 atccaggtgt gggttgggtc ttatggcgtg accaacaata cttaccagaa gaattagtat
     4681 ttaaagttag ttacttaggt ggagaaatgc caactatggc tatcaacttc tctcatagtg
     4741 cagcacaact aattgggcaa tactacaact ttgtacgcta tggctttgat ggttatcgtg
     4801 atattcacca aagaactcat gatgttgctg tttatttagc caaagagatc gaaaaaactg
     4861 gtatttttga aatcattaat gatggatcag aattgccagt tgtgtgctat aagctgaaag
     4921 aagatcccaa tcgcgaatgg acactatatg atttatctga tcgtctgtta atgaagggat
     4981 ggcaagtccc agcctaccca ttacctaaag acttggatca attaattatt caacgcttag
     5041 ttgttcgagc agactttgga atgaacatgg ctggtgatta tgtacaagat atgaaccaag
     5101 caattgaaga gttgaataaa gctcatattg tttatcataa aaaacaggat gtgaaaacgt
     5161 acggctttac tcactaaaat tcttcagaga aatataaaag aagttaatta actaataaaa
     5221 ggagttgtga ttaccacaca gctccttaaa tctatttgaa taaatatatg atattcattt
     5281 gttacactct caatcttagc tgttgttcta ttcatcatta ctcttattgt aagtagttca
     5341 gtcaaatttg cttgttcaat tgatcgataa cagtatgaat tgtttgaaaa gtaatcggat
     5401 atataattgt tttctcacta cctaagcctt cttggtataa agaactatcg caaagtataa
     5461 tccgctgagt acaaaagaaa gtgtcgtcta ctatttctct aagttgttta caaatgatat
     5521 tactaataga aaagtgttct cccaaaatgt cttctaactg ttgtcgatag tataaatacc
     5581 taaagtctga cggagctact atgaacactt caattggaga tttccgttta tgctcattta
     5641 aaagacttaa actaatggtt agtttacgaa tagaattctc attccaacgc agaaataaat
     5701 tgttttgttt tcgccattgt tcaagaattt catgaacttc taagtacaaa gcgcgttgct
     5761 cttcatttaa caagtaagtc ttatcaggtt gaaatatctg aatatcagcc caaagtgagc
     5821 ggataaacgg gagaagtgct ttatcaaaaa gtatctgatt ttcttcagat atattcagag
     5881 ataagataag tttttgactg agctcaatca ccaaagggtt atctgctaca tggttttgat
     5941 aaacaacgtt caagtctttt tgtaacaatt caaagttatc gcatagataa tttctagagt
     6001 tgaatagaaa gtagatataa aggcattcat cttcgtcatg acataaagag aaagaaagtt
     6061 gctctaatcc tttctgtatt agctgaaaca agggagtatg tcgggcaact cttttttctt
     6121 cctgactaaa atatattggg ttagtcttag atctctgcaa accgatagcg atacctaata
     6181 aaataaattg tttttcaact gtagggtaac ttcttgaaaa aaaatgtttt tctacataat
     6241 caatcaattg ttgaccgctt tgaataattg atttatttag ctggatcaac tctgtccgat
     6301 ttgtctcata gattatcgtt agtaacaaac ttcgataatc cttttcagga atttcaaaat
     6361 ggccatcagc atttaagtac tgcatttgtt taaaaaaatt aatcaactca tttcgtaagt
     6421 tgtaaacttt gcttaacgaa atgaaggtag tatcagctat attactccaa ttgtgttccc
     6481 ctgataaata atgtcctagt acattgaaaa agtcagagcg ctgatagatg agttgagtga
     6541 gtgccaacct cgaaaacgaa gagtcgaatg cgactgtaaa gctacttcta gatttctcca
     6601 ctgaaataat ttgctgatgg agtatgtcgg taatgcattc caagtcgtta gtgacagaca
     6661 gtgagtgggc aaacgttcaa ttgatctgct atttcgcgaa tctgaatggt ccctttttcg
     6721 tgtaattgct cacaaatgta aacttgacga aaaatacttt tctccagata ggtttccaac
     6781 atagtactcc tcctagtcat tttatacata actattataa ctctaccaat cggaatagtc
     6841 ctgtaatata ttgaatcaaa aatataaatt ataaaataat tagatggggt atttggttta
     6901 agtatccctt atatagtaat aaatttatag gtgtgattct cgaattaata cccgaaaatg
     6961 tgagagaatt tttatggatt ttttaggata caaattaatt atttttgctt ttatcctgat
     7021 taccagtcca gtactgtcgg tatcccattt tgcagcaaaa tcatgttgat aaagtgacaa
     7081 gaagactgat gagatatatt atccaaattg agaagataat taatcggatt cttgagtata
     7141 ccctactttt taagaaagta gggtatacaa gcgtatgaat aaagacataa ctttccctgt
     7201 gatggacgtt cggcttgttc caattaccaa aatacaggcg aacgattata atcccaatcg
     7261 agtagcgtct ccggaaatga acttattgga gctttctatc attgaagatg gctttactca
     7321 accattagtt tgttattaca aggaaaaaga agagaagtat atcttggttg atgtgcttca
     7381 taggtatcgt gttgctaagg aaagactcaa cttaaaagtt gtaccggtaa cagttattga
     7441 taaacccata taagaacgta tggcttcaac aattcgacac aatcgtgcaa gagggaccca
     7501 tgaaataaga ggaatggaaa ttgtcatcca aagattagta aaaaatggat ggtcagacaa
     7561 gagaatatcc aaagaattag gcatggacat tgaagaaatt tttcgcttta agcaagtgtc
     7621 tggtggaatg gcaacagttt ttttgacaaa ttttataagg tgcagaactt ctttccgtat
     7681 gctattccga ttgtccttga caatgagcct cctcggatgt gacgatcttc ggcaggagct
     7741 aacagttaaa gttagactgc ctcgctagcg ttagcacatc cgagctcatt atcaaggatt
     7801 cgctgcgcca atctctggtt acggtaacca accggtgtct cgtagccaag tgtaccgtgc
     7861 aaccgaaggt ggttccacca atttacatag tcaaataact ccaaatccaa ttgttgtaag
     7921 gtttcaaatg tgtattgata gacaaattct actttcaacg acttataagt tgattcagct
     7981 acggcattat caaaaggaca gcctttatga ctcaatgatc gattgatgtc aaaagttgtt
     8041 aataattcat caatagcttg gttatcaaac tcttttccac gatcagtatg aaaaatctca
     8101 acctctgtca gaggttgttt gatacggcta aatg
//
LOCUS       DANJJH010000108         8093 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000108 DANJJH010000000
VERSION     DANJJH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1050)
                     /locus_tag="PWS53_002467"
     CDS             complement(<1..1050)
                     /locus_tag="PWS53_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL0872656.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTA"
     gene            complement(1050..2186)
                     /locus_tag="PWS53_002468"
     CDS             complement(1050..2186)
                     /locus_tag="PWS53_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0872657.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2218..3669)
                     /locus_tag="PWS53_002469"
     CDS             complement(2218..3669)
                     /locus_tag="PWS53_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0872658.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3713..4477)
                     /locus_tag="PWS53_002470"
     CDS             complement(3713..4477)
                     /locus_tag="PWS53_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL0872659.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4505..5203)
                     /locus_tag="PWS53_002471"
     CDS             complement(4505..5203)
                     /locus_tag="PWS53_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL0872660.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5215..5994)
                     /locus_tag="PWS53_002472"
     CDS             complement(5215..5994)
                     /locus_tag="PWS53_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL0872661.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6010..6954)
                     /locus_tag="PWS53_002473"
     CDS             complement(6010..6954)
                     /locus_tag="PWS53_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0872662.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7000..7779)
                     /locus_tag="PWS53_002474"
     CDS             complement(7000..7779)
                     /locus_tag="PWS53_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872663.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2304 a         1698 c         1221 g         2870 t
ORIGIN      
        1 tgctgtttga atattataat ctgcctttat taaaatcgaa cgttctgaat ctcgatgaga
       61 gatatctaca tttgcaatct ctctcgccca ttcttttgcc gattgattta aacttatgaa
      121 attggtctta tcactgaaag caacttctgt aggaactttc tccgaaaaga aacacggtaa
      181 atctgcaaat tgagcttcaa tacccactaa cggaacccct tcaaataatg atggcaacat
      241 taatacgtca aatgcttgat aaaatttttc tatatcattt ctatttccta aaaatttaat
      301 atcatcatct atgccacttt tctttgcata attttttatt tcttcttcca tatcaccagt
      361 tccaataatt aataatacgg aattattctt aatagcgtga aattctttaa aaatttcaat
      421 caaaaaacga tgatttttag aatatgaaat tcttccaata tgtcccatta caaacgtctg
      481 acttgaaatt cctaatttca ttcgtacatc atttctaata ctttcatcat aataaaattt
      541 tttagcatcc actgcatttg gaatcaaagt gaatttatca tttcgaaaca aaaaatcacc
      601 tgctactttt ccacatgaaa attcatgtgt caaaacagac gttaacttac atcgataata
      661 ttgctttaaa agatatttaa aatctctatc tatcgatgta ttatggctat gtgctatccg
      721 tatcggaatc ccagcttttt ttgcagcttt taaaggtaaa taactcatcg catcaatatg
      781 agaatgaaca atctcatatt ctggatgatc cttaaaaaat tgttccatcc atttaaaata
      841 tttaggataa ttttgtggat aaagtctcgg tgcataatag acctttcctc caagacttat
      901 tatttcctca tcataatcac ttttatatgg acgatgtgtc aaaaaatcaa actgaatttt
      961 agttcgatct atattcctat aatagttcat tagcatcgtc tccaaccctg cacgatgcat
     1021 atcattcaca cactgtaata ttcgaatcat tatatatttc ccctatacaa atttaatgtt
     1081 tcttgtacga ttttatccca attatattta ctacaaataa actcagtagc ttgattcttc
     1141 aaaactttaa ccatttccga ttgattgcat gcctcttcta atcttatctt taaatcactt
     1201 acatccgatt tcttaaaaat catagccttg tcttctacta cttccgtaca ctctgagata
     1261 tttgaaacta tacaacagtt cccataactc attgcttcta acaaacttaa tggcattcct
     1321 tctaaatcac ttggaagtgt atagatatac gcattactat ataattcttc taattcctgt
     1381 ccttgtacaa atccagtaaa tataattcgc tcatcacctt ttgctaattc ctttagttca
     1441 tttgcaaatt catctgtatc agagcttcct ccggctatta taagtttttt atctgtctga
     1501 acatctttaa atgcctcaat tagataccga attccttttt cgggaaccaa tcgccccaaa
     1561 aacaaaatat aagaatcttt tgttaatcca tacttttctg taatcaatcc tgctgatttt
     1621 atttcaattt ttttaacgcc atttggtata aaccttgttt ttcttccata tatatcttca
     1681 aaataatttt ggacgctttc actaagtaca attatttcat ctgcaaattt tacagcacac
     1741 ttttccccta acatgatgta tgtactagca agcttgttcc atttttctct ttgatgatct
     1801 agaccatgaa ttgttgcaac acatcttttc ccaaaaagtt ttggcaacca caacatagca
     1861 catggtcctt ctgcatgaaa atgaacaaca tcatattttc cgaatgctgc tctaattcca
     1921 ccaaatactg aagaactcat tgctgcaatt ccttttatat ttaatgtaaa aatagtcttt
     1981 aattttacac ctttatattc tttattttta tgctggtcaa attcctttcc gctaacatga
     2041 tgaccacttc gattatagca agtcacttca tgcccaagtg caaccattcg cgttgccaat
     2101 tcttctacca ctatctcaat tccaccttct cttgatggaa ttcttttatg acctagcata
     2161 cagattttta atttttttct tgccatcacg ttttactcct tctatagtat ctttatctta
     2221 catggaaccg tttctatgaa gcacagtttt gactgtccta aacaaaattc taaaatctaa
     2281 tcccatactc caattcctga tatattctgc atcaagtttt acaacctctt cgaagtctgt
     2341 gatatcactt ctaccactca cctgccacat tccagtaatc ccaggtttca tcgcaattct
     2401 cgcacgatga tgaaaatcat acttctcata ttctgacttc aagcaaggtc taaaccccac
     2461 caggctcatt tttcccatta ggacattata aaattgtgga aactcatcca atgatgtaga
     2521 tcgaataaat tgtccaattc ctgtcttctt tgttccatca ggaagtattt tattgccaat
     2581 gattcttggg tcaaaatcca tcttaaacat gagattatta ctcatcttat tttctttcat
     2641 caactctgct ttgcgttctt ctgcatccat atacatacta cggaacttat acatcttaaa
     2701 tgtcttacca ttttgaccaa tacgttcttg tgcaaagaaa atcggccctg gtgatttgat
     2761 gtaaataact ggtgctacga agataaaaat aacacctgtt gcaatacatc caataattcc
     2821 agcaagaatg tcaatcgttc tctttataaa tgcttgcctc atcgtcataa cattaatact
     2881 agatgtaata acagtataat caccaactcg gccaataaat tgttttccac gtgtactatc
     2941 agacactttt gtaagattca tatgtacagc aagtcccatt tccgaacatc tggcgattaa
     3001 ttctttggga tatggaacac tttcgtcaac attgatgaat acttcatcta cccaattctg
     3061 taaaagatat tccgctgcat tatcagcatt tgcaactact gggacacctc cgatggtagt
     3121 tccatacata tctctgtcta ctacaatcac accactaagt ttatacatgc cataattctt
     3181 ctctttaaga ttacgaacta cattttttgc aatcttttga gatgttacaa taactaacga
     3241 tacatcttca ccgtttttca tgcggtgatg caacaaccct ttccaaagta tacgaattat
     3301 atatgttaat aatccataca ccacccccat tataaataaa gaaactctgg aataatcatt
     3361 tccatcctgc attgtaataa ggtataaact gcacacaagt aacagcataa aagattgctt
     3421 tatagaagct acagcctctt tataatatcc tctttttaaa acatttttat atgcctcaaa
     3481 taaaaatata atcgtaagat tctcaagttc tataaaaatt gccatgtggc gataaatagg
     3541 aatcacatac ggattccagt caccctgtcg cattacatat gatatataaa aagcaagctg
     3601 gacacaaaat aagtctaata aaataaaatc aatatgttta gaccaaccac ttgagttttt
     3661 tctatacatc tctcactctc tcctcatttc ctttttatct acgaataaag aactaaaata
     3721 aaccaaactt cttctttttt acctcttcat agattggata attgatctca tccccgttga
     3781 tcagatcctt tgcgacttgt ttcatctgta ctactttttc cttaccaaac tccttggcaa
     3841 tctgctcata gcactctttt aagtaaaagg tgcgcttaga gaccccgtgt gcatcagaag
     3901 cagccatctg cacgagatta tgtttgacca ttgttttggc tgttttctgg atatctttgc
     3961 caaatacacc aacataactt ggcgcagtaa gttgggccaa acatcccata tctaggaacg
     4021 gaagcaaatg atttgggtct tctcgaaaat gcgcgttgcg ttctggatgg acgatcactg
     4081 gtatctttcc tagttctaac aactcaaaaa aaagccgttc tgcatataac ggcacttcca
     4141 gtgtcggaaa ttcgatcaga agatatgtat catctaaatc tgtaaataaa atttcatctc
     4201 ggtggatgtc ttcgatgagc gttccggtaa tacgtacttc ttgtccttcc aataacgtca
     4261 aaggcaactg tcttttctct aattcggctt gtaacgaagc gactagagaa atgacttgtg
     4321 atttttcatt gctgtatttt ccattattgt ggtgtggtgt gcataaaata tgcgtaatgc
     4381 cttgctgaat cgctttttca gccattgcaa tacttgcttc taagttttct gccccgtcat
     4441 cgattcctgg taggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttctta
     4501 ttccttaatt tccgtagtaa taaccagcat ctttgctgtg ttctgctccg ttgtatacga
     4561 cacctaagac tcgtgcttgt accatatcta acagttcttt tgctttagtc aatgattctt
     4621 ttcgtgacgt attttcccgt acgaccaata aggtgccatc cgtttttgaa gacatgatct
     4681 gtgcatctgt tacggcaacg actggcggca tgtcgaagat cactacatca aatagttgtc
     4741 ttgcttctgc taaaatctga tccattcttg gcgaacttag tagttctgat ggatttggtg
     4801 gtttaggtcc acttggcaga atcgacaaat tgtcgactgg tgttcgttgg atctcatctg
     4861 ctacacttcc tgatgaacta agaacagtac ttaatccgct tgcgttgttc aacttgaacg
     4921 ttttatagat gacgggctta cgtaagtctg catcgactag cagcacccgt tgaccagatt
     4981 tggcaaaaac aacggcaatg tttgctgcag tggtcgattt cccttctcca ggtcctgatg
     5041 aagtcacaac aatcgttttg atttgctgac cagctgcgga ggcaaactgg atattcgtcc
     5101 gaatcgtacg atattgttca gcgattggtg aagagggatt ggtgagtgtg accaaactga
     5161 ctgcatgagt ttgggctgct ttttgtttct gtgttcgtgc cattttcttt cctcctagac
     5221 tcttgagcgg cttctgcgtg atggcgctgt tttatcgttt gtagcttttg gttgtgtctg
     5281 agctggtcct tttttacgga taccagcatt caattctttc gctgacattt ctggaactgt
     5341 tcctaagatt gggaatccta atgtatctgt cacatatttt tcatctttca ctgtccgatc
     5401 gagtaattcc aataagaatg ccagacctac acctaccatc attcctaaca ttaagccagt
     5461 cattaaattc aatttattat ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat
     5521 gatcgagatt ttatcaacat tcatcacatc ttttgcgttc tcttggaaaa cttgtgaagt
     5581 tgtgttagcg atctgttgag cagccactgc attcgtactt attgcctgaa tcgagaacat
     5641 ttgtgaattt tctgcctgat taactgagat ggcatctttg atttgttcag cagtcatttt
     5701 cacattgtct tctgtttcta aacgatcttt gacttcattg agcactaaat cactgacgat
     5761 catatcttta tacgtattga tcatttgcag gttcgtattg acatcattga catttgcagt
     5821 gtctgattgt ggcagtgtca cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat
     5881 gaaaaatgtt acgattcctg agatggccaa gccagcaaac attgagatga tgattattac
     5941 caagtgtttg cgtaatgttt caaaaatttc tttcaagctg attgtctctt ccattttttc
     6001 tccactttcc tatttattct ctagattcat ttgatctttt aattcttttt tcacacgatc
     6061 aagttcttga tcactgactt tttgatagga gatattatct tgcatgaagc cttctccttg
     6121 cagctgatct tgcttaatcg taccaaatgc ctcccgataa tctaaagcaa tttttttcat
     6181 ttcatcaaag ttcatgtctg ttttcatatt cgactctaga gcattcaaaa cggattgata
     6241 gttgctgaca ccgtttagac tcaatacttt tttcgctacg ccttctacga tctttctttg
     6301 gcgcccttgt ctaccgtaat caccatttgg gtcttcataa cgcatacgag aataagataa
     6361 tgcctgatcc ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa
     6421 agtgaggtca ttattgactt cgataccacc aactgcatca acgagttcct ttagcccttt
     6481 catattgata gagacatagt gatcgattgg gatgtctaca tatttttgta cggtatccat
     6541 cgccatggca gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc
     6601 tacgatgtcg acatacgtat cacgaggaat gctgacgatc gttgtctgtt tgtcttcagg
     6661 actgactgtg gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc
     6721 agtatcaatc cccatcaata gaacagagaa agattcttgt ttgtccagat caactggttt
     6781 cgttcgttct ccttgggttc gttccactga ttcgtaagtt ttctcaatag aatgtgagag
     6841 atccagatat aattttgctc ctactcctac aattgctaat actaatacga taagaactgc
     6901 taaaataatc agaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc
     6961 ctccgtttgc taaccttgct tgaatcaacg ttttaactgt caatagctta tattgctata
     7021 gcccatttgc tcagcaacag tttgtgcttt tgttgcgtat gctgcgctta ttttttcatt
     7081 ccggatctgc tctatttgac tgagcaccgt caaatattga tcgtaagtag cggtggcagt
     7141 cacttgacca tcctttaaca tcgtatcgaa taactgatct agttgcgtga cttgcgcgat
     7201 ctgaatatca gcgaatccta gatattgcat aacaaccgtt ttccaaggac tatctttaaa
     7261 aaagctcata ttttcacgta cacgagcgac atctgcactt gtcacctttt ctttgatgat
     7321 gacatcgtct acagcttgct gccagtttga tacattttta gtaaataaat cgttgacagc
     7381 tgtctggatc ttcagttttg attctgcact atctaactgt gtcacgattg ctttcttctc
     7441 atcttggatt gcaacagctt ctttaggcaa ttctgattct ttgatgtcgt aatcatctgc
     7501 tgaaagtttg acagaggata gcttatttga caaacttgtg attgtttctt ctgttatttc
     7561 agggtttaga aaaatttttt cttttgtact atacagcttg tctacttctt ttgataaaga
     7621 agtcaaggtg tctttttgat tggtgatggc agattctgca taattgatcc gttgctgatg
     7681 ttgccgctga ttattgatat ggtatccaaa caaacagatc gcaatagcac ctgcacctac
     7741 cgccacagca gtcaatgact tgacgaatgg cttttccata gattcactcc taagtattga
     7801 tgatagataa attaagatat aattcactta cataaaaatg acagcttttt atttaaaaaa
     7861 acataattaa actaatatat tcacagaaat atttgtaaac aagttttccc tatataatca
     7921 taccacaaac tgttttcttg actagggcaa tttacgattt aaaagtaaat aaaacattaa
     7981 taagataaaa attaatcgat ttcattttta tttccataat tttcataggt gtttttaaaa
     8041 gttacacaaa aaatagaata acaaaaaaat taacattgaa ttaataatag tta
//
LOCUS       DANJJH010000109         8051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000109 DANJJH010000000
VERSION     DANJJH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            94..1248
                     /locus_tag="PWS53_002475"
     CDS             94..1248
                     /locus_tag="PWS53_002475"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL0872664.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="PWS53_002476"
     CDS             complement(1270..2022)
                     /locus_tag="PWS53_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0872665.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="PWS53_002477"
     CDS             complement(2386..5052)
                     /locus_tag="PWS53_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0872666.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="PWS53_002478"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="PWS53_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0872667.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="PWS53_002479"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="PWS53_002479"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL0872668.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2332 a         1678 c         1443 g         2598 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata t
//
LOCUS       DANJJH010000110         7792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000110 DANJJH010000000
VERSION     DANJJH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..348
                     /locus_tag="PWS53_002480"
     CDS             <1..348
                     /locus_tag="PWS53_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872669.1"
                     /translation="FIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLK
                     PPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFP
                     NKNNNFYFYRNKL"
     gene            complement(474..1151)
                     /gene="nth"
                     /locus_tag="PWS53_002481"
     CDS             complement(474..1151)
                     /gene="nth"
                     /locus_tag="PWS53_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL0872670.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1158..1856)
                     /locus_tag="PWS53_002482"
     CDS             complement(1158..1856)
                     /locus_tag="PWS53_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0872671.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2124..3308
                     /locus_tag="PWS53_002483"
     CDS             2124..3308
                     /locus_tag="PWS53_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL0872672.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3314..3757)
                     /locus_tag="PWS53_002484"
     CDS             complement(3314..3757)
                     /locus_tag="PWS53_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0872673.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3983..4270)
                     /locus_tag="PWS53_002485"
     CDS             complement(3983..4270)
                     /locus_tag="PWS53_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872674.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4441..5793)
                     /locus_tag="PWS53_002486"
     CDS             complement(4441..5793)
                     /locus_tag="PWS53_002486"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL0872675.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6066..6512)
                     /locus_tag="PWS53_002487"
     CDS             complement(6066..6512)
                     /locus_tag="PWS53_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0872676.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6748..7674)
                     /gene="cysK"
                     /locus_tag="PWS53_002488"
     CDS             complement(6748..7674)
                     /gene="cysK"
                     /locus_tag="PWS53_002488"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL0872677.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2107 a         1583 c         1270 g         2832 t
ORIGIN      
        1 tttattattt tttctgtact tttaagcact actcttaaag gaaaatttat ggaaagaagc
       61 tctcatgcaa tgattctcac ttttttatgg ttgttgttca ccagaatcta tatttttctg
      121 atgagcctga aaccgcccaa atccattagt ttatgtttag ttataaatgg tattttttta
      181 ttatcactta tcatttttat tacaccttta gatagatacg tcacttattt atataatcct
      241 ttggaatact ggacttattt tataggtata ttggaaggca ttattttcta catcgggtat
      301 tttcccaata aaaataacaa cttttatttt tatcgcaata aattgtaata agttaccatc
      361 ctagctactg agtatgtaca tttacttttt catgcaaaaa agataaacga aacagctttt
      421 ctccataata acaagataat tcatacattt atttgcaatt aaagaaacca cgtctacagg
      481 cgtggtttct tttttagcgc cttttctttt agacgcatcc gatttttccc atcttgacat
      541 attgatagca aaggacatac ctcgcacttg ggatttcttg ccgtgcaatg atatctacca
      601 aaaaaaatca gtgtatgatg tgtctttacc cataattctt gtggtacttt ccgcatcagt
      661 gtttcttcta cttccatgac cgtagcatct agtttgcaga tcctcaggcg ttttgagaca
      721 cgttccacgt gggtatctac tgcgattgct ggtatcccga atgcatcccc caatacgaca
      781 tttgctgtct ttctgcctac accaggaaga gacatcaatt cttctcttga cgttggcact
      841 tgcccatcga aacgttcaat cagctgctgt gcacacgctt tgatgttttt tgctttattt
      901 cgatacaaac caatcgtttt tattttaaga atgatttcat cgattgaagc ttctgctaac
      961 gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg ctttattcac tgatacatct
     1021 gtcgcctggg cacttaagat cactgcaatc aataactcaa atggattatt gtgcttgagt
     1081 tctccatgtg cttcgggaaa cattccatac atcgtttcta gagcttccat tgttttttgt
     1141 ttgcttaaca tattccgcta ctcctccaac caatttttca gtgagacttt cggcaattct
     1201 tcttgttgag ctgactgttc tacttctctt tgaagaagct gctgttttct tcgtttttgg
     1261 tcttcttcca cttgttgttt tgtacgtaga ttttttcttt cccacgatag caaaatgcgg
     1321 tcaacgtaat taaaactata agcctgattt aatacggctt cccgcagtgc tagcttcaaa
     1381 atttccggct gataatggtc ttcttctagc cattgtccga tccgctggaa ctcgatcgaa
     1441 gaaagcgggc gtccaaattc ttgttcaaat aattgataca ctgactggat ttctttttct
     1501 tgtgtttttt cttcgtattt tttttcttgt gtttttaaat actcacctaa tagatcatag
     1561 atggggtaaa gattatatcg atctgctttt ttaccgttga caatcgtcgt ttctatcttg
     1621 ataaatccat ttgttacgag acgatccagt atttgaaaaa tctgatcttg tttgattccc
     1681 atatccatag caatcatctg caaatcaggg aaagtatctc cttcaagctg tgccatatgc
     1741 aactgtagaa caaataaaaa ttcttcagat tgtagaccga tcctatgata atttttaagt
     1801 aaaagattag atatgactgt ttgtccagcg cttaagtaat tttctaaatt caacatgatt
     1861 tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg gaagcaagaa caggtttctt
     1921 ttctatttac ttcactgatc ggtcagattt acaatgtcgt ctacttgatt tttgtttgtt
     1981 catgtttttg atgcgtatct tcacttttcg gaataaataa attaagtaga tttcgctgtt
     2041 ccttagaatg ttgacaagga aacaaaatta ctatacacta tttttgtttc caagttaaca
     2101 ttattgcata aaggagtttt tacatgtaca gtttcacaaa taaacattct aaagtttttg
     2161 gatacgtctc tgtcttattc actggattag gatttactat cgtcagtcct gtacttccgt
     2221 ttttaacgct gccttattct cactccatac atcagcaagc tttctatatc actttgctca
     2281 tgtctgttta tgctctagct gccttcttat ctgcccccat tctcggtagc ttaagtgatc
     2341 attttgggcg tcatccgatc cttattatca gtttattagg atcaagtatc ggatatttgc
     2401 tttttggttt aggaaattcg atatggatgt tgttccttgg tcgtatcatt gaaggattga
     2461 cggcaggaga aatctccacc ttatatgctt actttgccga catcacggaa cctaatgaaa
     2521 gaaccaaagt ttttggatgg atgggtgctt tagtcggtat aggaacaacg ctgggtccta
     2581 ttataggggg tctattagct gagttgggga attctgttcc tatatttatt ggagcactct
     2641 ttactttctt aaatgccgtt tatggctaca catttatgct agaaagcctt ccaataaaaa
     2701 aacgttcagt agatttaagt ttctcccatg taagaccttt tcaccaatta caacaattat
     2761 tcaagatttc atccgttatt cctttattga cagcaggttt tgctgtttgg ttggcagctg
     2821 gctctcttca atctatcttt tctcaatttt cgattgacgc ctttcagtgg aaggctgggt
     2881 tggttggttt aagcttctct ttgattggaa tcttggatac tgtctcgcaa ttgttcatta
     2941 tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac aagaataggg atgttcagtg
     3001 aaatcctcgc ctatctattc atcacattat ctggaattct tctactgccg attttgtttt
     3061 tatttgggat tatctgttat ggtttcggtg attctatttt tactcctgta ttcaatggcc
     3121 aactatccaa ttctgtctca gataaccagc aaggtcttgt aatgggcggt acccaaagta
     3181 tacaatctct atcgcgcgtc atcggcccgc taatcgcagg acagctttat gctgcagcac
     3241 catttcttcc gacggtattc ggatttttgc ttatgatttt tgcctattat cactacaata
     3301 aaaaatagta gcttcatttg ctacttcgta taaattctct gtatcgaccg ataaaacgaa
     3361 atactgtgtc aaattcttct tttgacatat tttcaaatac agtagcatct cgctgtaccc
     3421 atttttgatg gagttgctcg tgtctattcc ttattttttg tccgattggc gtcaattgaa
     3481 aatagatttc ttttttattc tcttcgcttt gataagtgtt tatgatccct ctagctatca
     3541 gtttttttgt tagcttactg atggctcctc tagtcatgta actagcattc gctaattttg
     3601 tcacgttagg atcagggatt ttcccgatat gttcaatcac ttctagttca tttagagtaa
     3661 atcctgcaaa ttctttttcc atttctggtt tattacgatg aataatctca ttagctaaat
     3721 ccagatattc ttctattagc agttgtcttt tatccatttc tacaccttct ttttctttca
     3781 ttatattgtt tttttgtttc ctattcaaca aagataaaaa tattttgtag gaaaacccaa
     3841 tctgaaacca agaaaaaaag gggttgacaa agcttgtcat aacccctttt tctgattaaa
     3901 agatagtaaa gagacagtca tcggtaattg tgatgatttt ttgtcatcac ttaaggatga
     3961 gcggcctttt caaaaactgt ctttaaagat catcatcagt ttctagcaca tatggctgac
     4021 cttcatatgt gtactctgta ttcaatgtgc cgtcattttc atctgagtag atgcgtctac
     4081 tgaaaggaaa acgatgagaa acattatctt tggcgtcatt tgctggcatc gtgtaatatt
     4141 cctcttgcgt ttcattcaac tgagagagta cttgatctgc caactcatca atattgatcg
     4201 ttaatttctt cgaagcactt ttataatctt ctaaaaatgc atcttgttgg acaaaatatt
     4261 ctttattcat cttctttctc acctttcttt tcaaaatcaa ataaagctgc ttgttctttt
     4321 atgtaacagc tttatctttg ctgcttattg tgacaaaaaa ttcgaagaaa gacaactaat
     4381 tgtacttttc acaaaaaaag acagagggtc gtgacaaaat tgtgtcacaa ccctttttct
     4441 ttattgtgca gtttcgattt cgcctagcgc tgcttgtgca agtaggttca agtaattcca
     4501 tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt gcgagatctt ctacagtcat
     4561 tttattttga acagctaaag acatggtatt tgctgattgt gtaatgtcgt atttggacat
     4621 gaactgagca cctacgatac gattcgttcc tttttcatag actagctcca tcattacttt
     4681 ttcagtagtt ggcatgaact ctggacgata attatcttca aatacagtcg cttggacttc
     4741 tagatcgttc aaagttgcgc tttctttggt cacacctgtc gaaccgatcg tccagccaaa
     4801 taggtaaagt cctgaagttc cttgagtacc acgatacgcc attttatgat ctgtcaagtt
     4861 atttcccaca agcaaacctt gacgaacggc atttgttgcc aaaggaatat agttttgcgt
     4921 gccacttgga ttgtagttga ctactgcaga gtctccagct gcaaaaatgt ctggattact
     4981 tgtctgcatg tattcattca ctttgattgc cccgtttggc agtgtatcta cttttccttc
     5041 gatcagtttt gtatttggac ggaagcccac acataaaatg accatatctg tatcaaatgt
     5101 atctgttggt gtagctactt gtttcacttt tccttcttca tctgtgataa attcagtaac
     5161 attttcgcct agcgcaagag taacaccgcg gtctgtcaat tctttttcta agatatctgt
     5221 gaatggttta tctagatatt tattcaagat gcgatctaat ccatcaatca atgtcacttg
     5281 ttttccagat tcgacaaaag cttctactaa ttcaataccg atatatccac caccaacaat
     5341 tactacacgt tttgcttctt tcgcttgtgc aataatttca tttgcttgat tgtagttttt
     5401 gcataggagt acatttttcg catcgattcc actaatcggt gggataatag gccatgaacc
     5461 agttgtcatc actaatttat cataagaaac cgtttctgtt tctcctgttt tcagatcttt
     5521 tgccgttact gttttatttt ctgtatcgac gtctgttaca tcgtgttcca ttttaactgt
     5581 tgctcctaat gaagataatt cttcaggatt tgagtagaac aagcctgctg ggtcctttac
     5641 tactccgccg acataaagtg cgataccgca agataagaaa gaaatattgt catttctttc
     5701 atatactgtc acttctgctt ctggatgatt ttttaaaata ctttttacag ctgcagtacc
     5761 tgcatgagta cagccaatta ctactacttt catttatggt ccctccgatt aacattttgt
     5821 ccttcgacaa aactactata acagaaataa aacggataca acagtttaat gcttgtgaat
     5881 tcacaaaggt ttgtttgtag tttcattcct tttcaagcat aagaacccaa tatctacaag
     5941 attcttattt taataatatt cacttttata tctaagattg tgaataaaag acaaaaaaat
     6001 aaaaaagcta gcgttttttt tacacagcta gcttttttta ggtataacac ttgtagaaac
     6061 tgtttttaca tttgcgcctt ttttgctgac tctttacaat ccggacagag tccatacaat
     6121 tccaaccgat gttcattaat ctcaaaatca gtcagtctgc ttgcagccat ttctatatct
     6181 tcaagccctg ggtaatgaaa atcaacaatc ttaccgcagt tttcacaaat tacatgataa
     6241 tgacgctttg aactgaaatc aaatcggcta gaagcatcgc cgtacttcat ttcttgaaca
     6301 aatccaatcg aagtaaataa gcgaaggtta ttgtaaacag tcgcaacact catatttgga
     6361 aaccgatgtt caagtgcttt ataaatctca tcagccgttg gatgggaatg atgttcaatc
     6421 aaatattcta aaaccgcgta ccgttgaggt gtgattcgaa tgttctcttt ttttaacttt
     6481 tcgataacct cttcgactaa cttattttcc atgtaaacac cccacgttgt tattctcgtc
     6541 ttatcatact atttttacat tttaaaatcc acttaacatg aaaagaaaat tcaattttat
     6601 tgtattttaa tgaaaaaaga cgtaaaacag tcgcgttcac tattctacgt ctctattcaa
     6661 ataatttttc tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata
     6721 aacctatctg gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata
     6781 acgttcacca ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc
     6841 gacttttagc gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt
     6901 tcgcccaact tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc
     6961 atacacagaa gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg
     7021 aggtcccgct tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc
     7081 gattttcgga tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc
     7141 gatacctgca acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc
     7201 agtcgtcttc tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta
     7261 gccgttttct tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc
     7321 aggagtcaaa atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat
     7381 cgtctcaggc atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc
     7441 gattcctgta tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc
     7501 gtgttctgct ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt
     7561 aaaaaactca agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt
     7621 gacgattggc gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa
     7681 ttcctccttg tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt
     7741 gattcgtctt tgactagcaa aaaaaagaca aagatatacc atatatggta at
//
LOCUS       DANJJH010000111         7610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000111 DANJJH010000000
VERSION     DANJJH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..179)
                     /locus_tag="PWS53_002489"
     CDS             complement(<1..179)
                     /locus_tag="PWS53_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0872678.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="PWS53_002490"
     CDS             complement(192..1295)
                     /locus_tag="PWS53_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL0872679.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="PWS53_002491"
     CDS             complement(1385..2341)
                     /locus_tag="PWS53_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL0872680.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="PWS53_002492"
     CDS             2622..4556
                     /locus_tag="PWS53_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0872681.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="PWS53_002493"
     CDS             5013..5429
                     /locus_tag="PWS53_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0872682.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="PWS53_002494"
     CDS             5443..6009
                     /locus_tag="PWS53_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL0872683.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="PWS53_002495"
     CDS             6032..7018
                     /locus_tag="PWS53_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0872684.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7610)
                     /gene="scm"
                     /locus_tag="PWS53_002496"
     CDS             complement(7173..>7610)
                     /gene="scm"
                     /locus_tag="PWS53_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0872685.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2184 a         1458 c         1567 g         2401 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgttgtggt agatgtatcc gtactgtcgc ttgaagctga cgtgtccgtg
     7561 ctatcacttg tggtagaggt agtatctgta ctgtcacttg aagctgatgt
//
LOCUS       DANJJH010000112         7602 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000112 DANJJH010000000
VERSION     DANJJH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(59..787)
                     /locus_tag="PWS53_002497"
     CDS             complement(59..787)
                     /locus_tag="PWS53_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0872686.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="PWS53_002498"
     CDS             complement(800..2080)
                     /locus_tag="PWS53_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0872687.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="PWS53_002499"
     CDS             complement(2221..2823)
                     /locus_tag="PWS53_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0872688.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="PWS53_002500"
     CDS             3068..3274
                     /locus_tag="PWS53_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872689.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="PWS53_002501"
     CDS             3586..4305
                     /locus_tag="PWS53_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0872690.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="PWS53_002502"
     CDS             complement(4386..5069)
                     /locus_tag="PWS53_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL0872691.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="PWS53_002503"
     CDS             5287..7473
                     /locus_tag="PWS53_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0872692.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2722 a         1283 c         1428 g         2169 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa cacagtttat tc
//
LOCUS       DANJJH010000113         7600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000113 DANJJH010000000
VERSION     DANJJH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(943..1356)
                     /locus_tag="PWS53_002504"
     CDS             complement(943..1356)
                     /locus_tag="PWS53_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL0872693.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PWS53_002505"
     CDS             complement(1812..2297)
                     /locus_tag="PWS53_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872694.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PWS53_002506"
     CDS             complement(2436..2651)
                     /locus_tag="PWS53_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL0872695.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PWS53_002507"
     CDS             2875..3675
                     /locus_tag="PWS53_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0872696.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PWS53_002508"
     CDS             3694..5562
                     /locus_tag="PWS53_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0872697.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PWS53_002509"
     CDS             5555..6046
                     /locus_tag="PWS53_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL0872698.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PWS53_002510"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PWS53_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL0872699.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="PWS53_002511"
     CDS             6606..>7600
                     /locus_tag="PWS53_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL0872700.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANJJH010000114         7520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000114 DANJJH010000000
VERSION     DANJJH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(583..2214)
                     /locus_tag="PWS53_002512"
     CDS             complement(583..2214)
                     /locus_tag="PWS53_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL0872701.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2228..3901)
                     /locus_tag="PWS53_002513"
     CDS             complement(2228..3901)
                     /locus_tag="PWS53_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL0872702.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3914..4231)
                     /locus_tag="PWS53_002514"
     CDS             complement(3914..4231)
                     /locus_tag="PWS53_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0872703.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4256..5656)
                     /gene="lacG"
                     /locus_tag="PWS53_002515"
     CDS             complement(4256..5656)
                     /gene="lacG"
                     /locus_tag="PWS53_002515"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0872704.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5893..6678
                     /locus_tag="PWS53_002516"
     CDS             5893..6678
                     /locus_tag="PWS53_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0872705.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6681..7373
                     /locus_tag="PWS53_002517"
     CDS             6681..7373
                     /locus_tag="PWS53_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0872706.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
BASE COUNT         2285 a         1678 c         1327 g         2230 t
ORIGIN      
        1 tagtgtttta ctgttctgta tattgtttta actcccttga tagtatcgca ttctgtttta
       61 tacactttac gttgaaaaca gagtgggaat aaagagccaa cataatatgg agggggaatc
      121 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
      181 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
      241 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
      301 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
      361 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
      421 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
      481 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
      541 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
      601 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
      661 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
      721 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
      781 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
      841 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
      901 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
      961 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
     1021 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
     1081 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
     1141 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
     1201 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
     1261 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
     1321 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
     1381 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
     1441 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
     1501 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
     1561 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
     1621 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
     1681 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
     1741 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
     1801 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
     1861 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
     1921 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
     1981 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
     2041 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
     2101 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
     2161 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
     2221 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
     2281 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
     2341 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
     2401 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
     2461 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
     2521 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
     2581 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
     2641 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
     2701 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
     2761 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
     2821 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
     2881 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
     2941 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
     3001 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
     3061 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
     3121 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
     3181 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
     3241 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
     3301 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
     3361 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
     3421 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
     3481 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
     3541 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
     3601 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
     3661 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
     3721 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
     3781 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
     3841 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
     3901 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
     3961 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
     4021 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
     4081 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
     4141 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
     4201 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
     4261 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
     4321 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
     4381 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
     4441 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
     4501 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
     4561 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
     4621 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
     4681 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
     4741 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
     4801 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
     4861 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
     4921 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
     4981 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
     5041 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
     5101 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
     5161 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
     5221 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
     5281 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
     5341 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
     5401 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
     5461 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
     5521 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
     5581 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
     5641 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
     5701 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
     5761 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
     5821 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
     5881 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
     5941 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
     6001 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
     6061 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
     6121 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
     6181 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
     6241 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
     6301 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
     6361 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
     6421 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
     6481 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
     6541 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
     6601 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
     6661 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
     6721 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
     6781 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
     6841 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
     6901 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
     6961 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
     7021 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
     7081 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
     7141 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
     7201 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
     7261 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
     7321 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
     7381 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
     7441 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
     7501 tttttcattc ttttaaccat
//
LOCUS       DANJJH010000115         6985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000115 DANJJH010000000
VERSION     DANJJH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1..1201)
                     /locus_tag="PWS53_002518"
                     /pseudo
     CDS             complement(1..1201)
                     /locus_tag="PWS53_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1444..1899
                     /gene="tnpA"
                     /locus_tag="PWS53_002519"
     CDS             1444..1899
                     /gene="tnpA"
                     /locus_tag="PWS53_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL0872707.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2164..2262
                     /locus_tag="PWS53_002520"
                     /pseudo
     CDS             2164..2262
                     /locus_tag="PWS53_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2945..3763)
                     /locus_tag="PWS53_002521"
     CDS             complement(2945..3763)
                     /locus_tag="PWS53_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0872708.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3924..4613
                     /locus_tag="PWS53_002522"
     CDS             3924..4613
                     /locus_tag="PWS53_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0872709.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4627..6129
                     /locus_tag="PWS53_002523"
     CDS             4627..6129
                     /locus_tag="PWS53_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0872710.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6142..6618
                     /locus_tag="PWS53_002524"
     CDS             6142..6618
                     /locus_tag="PWS53_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0872711.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6715..6825)
                     /locus_tag="PWS53_002525"
                     /pseudo
     CDS             complement(6715..6825)
                     /locus_tag="PWS53_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2264 a         1101 c         1271 g         2349 t
ORIGIN      
        1 aagattttgt actccttcaa tttgtttcta taagctttca tttgatacgc ccaaccttga
       61 tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa
      121 ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa aatctcacta
      181 ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat gcctttggca
      241 tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg gcagatactg
      301 gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc tcgataagaa
      361 ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt cttgatcagg
      421 cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt ggtcatccgt
      481 aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc aatctcttga
      541 tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt agggaacttt
      601 aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat gattcgtgcg
      661 attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat tcttttaaaa
      721 aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg cgttcacgtt
      781 ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt ttcttagaca
      841 agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca cggaattgac
      901 gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca acttcacgat
      961 aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga acagaatatt
     1021 ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag gcattgatcc
     1081 agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa tcacttccgc
     1141 ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta tacttagcca
     1201 taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg gtcggtacaa
     1261 aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt tgtgcgtaaa
     1321 gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt tagcgataaa
     1381 aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga aaggagtttt
     1441 ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa gtatcatatt
     1501 atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa agatcttgga
     1561 aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc acatgcaatg
     1621 ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc cagttttatg
     1681 gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa tctcaaatat
     1741 aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt cggattgaat
     1801 caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat tcgatatatt
     1861 ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag tgagtgaaga
     1921 tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt cgaccccttc
     1981 tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata gacttgattt
     2041 taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa tttacggcta
     2101 ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat tagaaaatgc
     2161 tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt ggaagtattt
     2221 ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag agtatattta
     2281 cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat aaataaaatc
     2341 tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt ttaacaagtg
     2401 atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg aagtattttg
     2461 caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa caagatgatt
     2521 gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa acattcgctt
     2581 agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc
     2641 gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga gaggatgatc
     2701 cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt tctttcaagt
     2761 tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg taggaactac
     2821 agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat caagtatgct
     2881 ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc taagtggggg
     2941 tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt aattcaactg
     3001 aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata taaagttgtg
     3061 atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt tgtaaaacta
     3121 agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata atttttgcgc
     3181 catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca gataaagaaa
     3241 aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga aaatgagggt
     3301 ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa tcaagactag
     3361 tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg atcattttaa
     3421 tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta
     3481 aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt ttttgactaa
     3541 agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca attttttgac
     3601 aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca ctcatcgtac
     3661 tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt tcagacttcg
     3721 ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact cctctacaat
     3781 ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata ctccattttt
     3841 atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc caaaaataat
     3901 aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt atttcaaaag
     3961 gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag gaagaaagtt
     4021 taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata cgtgcaaatt
     4081 ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt ggaattatta
     4141 aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa gtagatgaat
     4201 tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat cgacatgatg
     4261 gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat cagttattta
     4321 tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga atagattttg
     4381 taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg ccagatatag
     4441 atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga aggattcata
     4501 ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg
     4561 ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt tagggggaga
     4621 aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc aattgctata
     4681 ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga gagtgctata
     4741 aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat
     4801 gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat tgctgtagga
     4861 cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta tctagtatat
     4921 actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac actagataca
     4981 ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa tcgttataga
     5041 aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat tccgattgtt
     5101 tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc accaattcaa
     5161 aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg aactttttta
     5221 tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat ttaccctttg
     5281 ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat tgctggagcc
     5341 caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt tacttatggt
     5401 actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc agcatgttat
     5461 gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct ttattttagc
     5521 ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat gtttttattt
     5581 gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt ttattttgct
     5641 gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga ctttttatta
     5701 ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc ttttggtatt
     5761 gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt taaggtagct
     5821 attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa caaaaataca
     5881 tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga aaatattgag
     5941 tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga gattattcaa
     6001 aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa
     6061 gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat attaggaaaa
     6121 gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca agaaactttt
     6181 tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc tgtcttttca
     6241 caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat ttatgctcca
     6301 ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat aaaaagcaac
     6361 tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca aggagagcct
     6421 ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt ggttgatgtg
     6481 gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt ttttccggag
     6541 aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg gaattcatgt
     6601 ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct aaataggata
     6661 tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat ttttttattt
     6721 tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat aaaagcttcg
     6781 aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata aaatatagag
     6841 agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca acttaggaaa
     6901 tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc actatatacc
     6961 aaatatagta aaatgctgaa agatg
//
LOCUS       DANJJH010000116         6484 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000116 DANJJH010000000
VERSION     DANJJH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            41..493
                     /locus_tag="PWS53_002526"
     CDS             41..493
                     /locus_tag="PWS53_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872712.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            507..1196
                     /locus_tag="PWS53_002527"
     CDS             507..1196
                     /locus_tag="PWS53_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872713.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1321..3672
                     /locus_tag="PWS53_002528"
     CDS             1321..3672
                     /locus_tag="PWS53_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL0872714.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3752..4147
                     /locus_tag="PWS53_002529"
     CDS             3752..4147
                     /locus_tag="PWS53_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872715.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4472..5089
                     /locus_tag="PWS53_002530"
     CDS             4472..5089
                     /locus_tag="PWS53_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872716.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5130..5429
                     /locus_tag="PWS53_002531"
     CDS             5130..5429
                     /locus_tag="PWS53_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL0872717.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5463..5630
                     /locus_tag="PWS53_002532"
     CDS             5463..5630
                     /locus_tag="PWS53_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0872718.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5675..6280)
                     /locus_tag="PWS53_002533"
     CDS             complement(5675..6280)
                     /locus_tag="PWS53_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872719.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2241 a          894 c         1277 g         2072 t
ORIGIN      
        1 atatatatta taaatttaat aagaaagcga ggtgagaatc gtgaaaaaaa cgatactaag
       61 tattttttca gtatttacaa tattaattgg gggctatttt cttttgaatt tagattctgt
      121 taattcaaac gctcaaataa aagatcaaag agtagctgaa cataataata aaaaaatgag
      181 tttgaccaaa aatatctatt ctatgtcaga tgaagaattg tataaagaat ttggtgattt
      241 atccggggaa cagttagaag aaagtattcc aggaattagt gactcagata aaattgttgt
      301 cttgtctgct ggtgggcaat taatgggaga tggcaaatcc gaattagtta gttataaaca
      361 aaatcacaga cagtttattg atgttaatac tgattccaac gctatttcga ttaaggatct
      421 aaaagagctt ttaaatagta aaagaaatga attaaagcag ataaaagaaa ctaattcagt
      481 ggaattagag taaaggaaga ttcattatga aaaagatttt tggtgtttct gttgggctac
      541 ttatgatgtt aacatttgga aatgcagtat atgctgctga gaaagaagtt atttcagatc
      601 cagagcagta cgcctcacga tactttgctg attttgaaga agaagataaa gtacttgcat
      661 tgccagaagg tggtttttta aaaggggaag ctaaaattgt atctatggat aatccagata
      721 taattcaagg ttcatataat tcagaaactg atcctgcttc agtttcagtg acagaagcaa
      781 aaaaaattgt tgctgaaaaa ttcaaaaatg atattgaaac tactccaatt atgcctcgag
      841 gagcatttcc acccgatgtt ggtactagcg gacaccttct tgtactagca ggaggaggaa
      901 gctataaatc acagccattt agtggtgcgg gatggcgttt tggaggtatg ggtgtaaaac
      961 cagctgatgg tacaggcaac tttttacgtt ggaaatccta cattgacgat ggaagagtag
     1021 gaaatgtaaa tcaagccaac gcaactaaat ctggttctat tcaaggaatg gcattgtatg
     1081 ctcgtgaagg attcaaatac tttgcaccaa acgggcctgg ttggacaatg aatataggta
     1141 tttattacac ttataatcct gttgcaggta cgtattatgt tgttcaaaat atttaataaa
     1201 acagcacaaa gagatttctt tgtgctgttt tattattttt agttgttgac aaacactaaa
     1261 atataaaata taatcaacac ataaacgaac gtatgttcgg caagaaagca ggggattttt
     1321 atggatgaga tggttttaag tactcagaaa tggctaaaca agaaatacag taatgttaca
     1381 gggtttgata aggttccaga aaatgggcga acagggtggc ctacaatcta tggacttatt
     1441 gaaggactac aagtagaact tggtattact aatctagtag ctaattttgg acctaccact
     1501 gaaaaaatgt acgataatca ggttactcct aaatggggca aaaatctacc taaaaatatt
     1561 gtatttctta ttcaaggtgc cttttggtgt aaggggatta atccaggtgg cttcgatggg
     1621 gtatatactc catatttaga tactgctgta aaagagttgc agactgatgc agggtttaat
     1681 ggaagcgctg taactggagt tttggatgcg aaatgggcca aagcgttatt tgatatgagt
     1741 gcttttgttc ttgttcccgg cggagatagt aaaattagaa aaatgcagca atggttaaat
     1801 gtaaattatt tagaatacac gggaataatg ccttgtgatg gtatttatca aagaaattca
     1861 aatcaagcat taatttttgc attacaagct gaactaggat attctcccag tgaagcaaca
     1921 gggtcttatg gtaatgggac aactagtaaa acttatcctg tgtctgaagg taactcggga
     1981 aactatgtaa gaataattca atatggatta tatgttaatg gttattttga agacggtaca
     2041 ttcgatggta ttttcactaa atatatgggt ttagaagtac tggcattccg aaaatttatg
     2101 gtattgcctg aatatactga aatagcagat gttgttgtaa taaaaggact attgtctagc
     2161 gctggagata ttagaagatc tgcagatggc gcagatactt ctacgcaatt aactagatct
     2221 caaatccaaa cattggttga taatgatata aaaatagttg gtagatatct tactggtact
     2281 gttggaattg gaaaagatga acgaaataag tatctaacta cagaggaatt gaataatatt
     2341 ttttctaaaa atttatctgt gtttcctatc tatcaagatg gaggtgcagc attagcttat
     2401 tttacttatg atcgtggctt atcagatggt aagaaggcta tagatgcagc aaaaaatcta
     2461 aatataccat taacaacagt tatttatttt gctgttgatc tagacatgtt aggggaagaa
     2521 attcttgcat atgctggaga atattttaag ggggtatctg ctgtaatgtc atatagcggg
     2581 tatcaaacgg gtgtttacgg aacaagaaat gtttgttctc aaataatcaa taacgggtat
     2641 gcttcgtttg catttgtttc agatatgtcg actggttata gtgggaattt aggttttcct
     2701 atgcctagac aatggtcttt tgatcaattt gttgaattta ccataggaag tggcaacgga
     2761 gctgttggta tcgatttaat cgctacatcg ggtagagata gcggatttaa tgagttatca
     2821 aatgatacta ataatgataa ttacattgcc aaatacaatc agaaggttat aaatcagatg
     2881 gtacgtgcgt atcaatatct atcacaagct ggtcttgata atccttggaa ccctcatttg
     2941 acattttata gatatgtaaa ttattctggt ttgtcatggg acatcatttc ttctcctgta
     3001 acagagcatg atagaaaaat ttatgatgaa tataggaata aattaactaa tagcgagggc
     3061 ctgtataatt attttataga tcctaatact ggttcaataa taggtttgcc tcacttaatt
     3121 gtgacgttac aatcacagat gtttctagcc gacacaatca atgatagtgt ttctgacttt
     3181 gcaggttggt taggagattt aatgacttgc tggggtgaag ttaagaaatt aggaatacaa
     3241 atggagcaag gtgtatttgc attagtaggt acggctgcag gtggaacatt tagtctagaa
     3301 gatttatacc aagatgttga cgcggtaaat gtttatacct tagcaaaaag aaattctgat
     3361 tcaacagctt tatcggcttt aaaaacgtac tattgtgata agggtgctat tgatagattt
     3421 aatcaatttg ttaagaacag atttggttct tcagataaaa tcgcttcagt aacaaatagt
     3481 ttattagaaa atgacgaaac agttgatatc ttaggtgtag ggataatttc atttttcatt
     3541 gataaaatag ttaataattc acttgggaca gattttactt atcttgattt tattggagat
     3601 gaggggaaag ttgcaaatgg attttctgaa aaaattattc aatttcaaga aaatagaagt
     3661 aaacttaatt aagaatcgtt tactatttaa acagtgtcct tttccttttt aaatatatac
     3721 gataaaataa gtatatgaat tgtaaaagga catgatgatt atggaagaaa tagaatggaa
     3781 taatctgact caaaataata aaaataattc tggatgtgga attatcttta tgtttgttat
     3841 agttttggga atagtagtac ttttaataac tgtcaagata gtagattact ttgcgaatac
     3901 tcctgaagaa caaatacaga gaaaacttcc tgagtttgaa agagtaccac cttatgatcc
     3961 aagagataat tcatttcaag taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga
     4021 agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa
     4081 ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta gaaatatata gtgttgtaat
     4141 agaataatag tgttaaaaaa attgtaaaag ttaaagaagt caaattctga tgtgaaaaat
     4201 aaaatatacc atggaagaca taaaagaaaa actggataac tagtttattt aatggcagac
     4261 cgttaaggcc tgccattttt atgtatttta gcggaataaa tataatcatg cgggagtgta
     4321 aacttactgt ttcaaaaagt atactttttg acatgccttg attttctttg tttatgtatg
     4381 gcataataac cacataatac aatttgaaac gtttctgatt taatctgatt gttttgaaac
     4441 aattttagtg aaaaaagggt agataaaaac gatgagaaaa tttttttata tccgggtcag
     4501 tagttatgat caatcgatcg aacgacaact gatttccgcg aaggaattag gaatccctga
     4561 agaatatata tttgtagaaa aagccagcgg caaagatttt aaacgccctg aatatcaatt
     4621 aatgaaacgg atgtttcgaa aaggagattt actctatcta caatcattag atagactagg
     4681 cagaaataaa caaatgattt tagaagaatg gcgagaatta actaaggaaa agcagattga
     4741 tatttctgtg ttagacatgc ctctgttaga tactgctagt tacaagcaac tagatgggtt
     4801 agagacgctt atctctgact taattctgca attactcagc tatatggccg aagacgagcg
     4861 gaaacgaatt aatagccggc aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa
     4921 attcggcaga aagaaaattg ctttacctcc tgaatttcct gcgatctatt ctgactggaa
     4981 agcaggaaag ataactgcag tctatgctat ggatcaattg ggcatgaaac gaaatacctt
     5041 ttatcgacgg gtgaaagaat atgaacatac cttgcaccac ttttcttgat agatataaca
     5101 gctattagac gggaagggag agtttgaaaa tggacttaac gaatcaaagg gtcgttttta
     5161 ctggaacgct gcagcaaatg acacgaaccc aagccatcgg tttggttcac tctttaaacg
     5221 gccattgtca atcaagtgtt acttcaaaaa caaatttcct ggtttgtggt cagtattcta
     5281 agtcgttgtt tgatgattct ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg
     5341 cgttaggtca tgagattacc attttatcag aagtcgaatt ttatgcgtta attagtcaac
     5401 agttgaaaga atggcaacgg tatttataat cgttagtaaa atttacaagg aggaaaattt
     5461 ttatgaagcg aaaatcttat gatcgttggg gcattgaaat aactagtttt aatcgaaata
     5521 ttgcttttca cgtggagtgt gactgtgggg aattagcaaa aagtgtttat caaaaaaatt
     5581 acttttactg tcctaaatgc catcgccgat acggggtaca tggaacatag aaacgaaata
     5641 gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct ttctttattt
     5701 tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact ggaatgcctt
     5761 gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa
     5821 tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat aatactcctt
     5881 gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt agcagtgata
     5941 aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa tctaaaaact
     6001 gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact gtatttttta
     6061 tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac
     6121 ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca ctggcttttt
     6181 cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga tccaaatttt
     6241 gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc tttttaaaag
     6301 tgatataatt tacatgtaaa atttacttgt agttttactg ctatcatagc atttttaaaa
     6361 ataaatttta tgtagactta aaatttactt catggagggt ttaaatggat gaaatcaaaa
     6421 gtaacaaaaa agcatttggc atttatttag gagaacctag tacagaacaa ttaaaccaat
     6481 tttt
//
LOCUS       DANJJH010000117         6408 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000117 DANJJH010000000
VERSION     DANJJH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..2599
                     /locus_tag="PWS53_002534"
     CDS             <1..2599
                     /locus_tag="PWS53_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL0872720.1"
                     /translation="TFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYNPIK
                     QEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEKR
                     IESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYNP
                     SRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSRV
                     KSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSNG
                     SCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFKL
                     NQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQT
                     NNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDSF
                     GREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVTK
                     ANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGGV
                     PLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLENY
                     FNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITKIE
                     ATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSY
                     EQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVN
                     IFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLP
                     SSLKSYFRL"
     gene            2781..3020
                     /locus_tag="PWS53_002535"
     CDS             2781..3020
                     /locus_tag="PWS53_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872721.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3036..4409
                     /locus_tag="PWS53_002536"
     CDS             3036..4409
                     /locus_tag="PWS53_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872722.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4413..6047
                     /locus_tag="PWS53_002537"
     CDS             4413..6047
                     /locus_tag="PWS53_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872723.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6210..>6408
                     /locus_tag="PWS53_002538"
     CDS             6210..>6408
                     /locus_tag="PWS53_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872724.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2245 a         1049 c         1289 g         1825 t
ORIGIN      
        1 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
       61 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
      121 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
      181 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
      241 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
      301 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
      361 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
      421 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
      481 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
      541 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
      601 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
      661 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
      721 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
      781 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
      841 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
      901 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
      961 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     1021 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     1081 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     1141 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     1201 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     1261 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     1321 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     1381 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     1441 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     1501 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     1561 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     1621 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     1681 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     1741 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     1801 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     1861 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     1921 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     1981 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattacaaa
     2041 aattgaagca acttttgtcg aagctcaaaa aaaactatgg gctgttttac ctgaacattc
     2101 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagccc tcctaattca
     2161 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     2221 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     2281 aacattgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     2341 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     2401 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     2461 ttttaggcaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     2521 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     2581 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     2641 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atagcgagca
     2701 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     2761 tgaaagaaag gaaaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     2821 tgtatattca agaacatttt tcaaatcaag ccttttttac aaaaccaatt tacttggatt
     2881 tcgaaataag aggggtatct gctggatcta tcgggggtac tcttcaagcg ttgaagaacg
     2941 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tagaagagtc gtgaaaaaat
     3001 ggtatttagt tcattcatag agaggggaat caagcatgcg tgtggcttgt tatgatcgaa
     3061 cgtctgttta tgaagaagcg aaaattagta atgacgctca atatgatgca gtgaaagagt
     3121 atgttttaga gaaatatgaa caaattcccc aacgattttc agacaaagga gaatcagggg
     3181 aaaaagcgga gcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     3241 agggggtgat ctatgtttgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     3301 aatttattga gttgtgccaa caacatcagg ttcaagtgta ttcgatttct gaacctcttc
     3361 ctgcttcttt ctcacacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     3421 cagaacaaca acgaaacgtc attattgaga atgttcgtgc cggactttcc tataaaaagc
     3481 aacaacggca atatttgtct tctgcggtac catatgggta tcgtttagtg aatgggaaaa
     3541 tccaacaaga agtacacgaa gcaaggattg ttcgctatat ttttcaactt tatttgacca
     3601 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagctt ttcttccgcg
     3661 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     3721 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaag
     3781 caaccttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     3841 atctgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     3901 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     3961 gctgccaagg aatttttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     4021 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaatta acgcaacaat
     4081 tagccgaaaa gcgaaaagat gaaaaacgca aagtaatcca acaaaagaaa caagaagcac
     4141 aatggattaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacaactgc
     4201 aagctcttca aaagccgatg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     4261 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattatta tgtgactatg
     4321 tttgtcggat tgaggtaaca aaggataaaa cagtttgtgg gatttacttc cgacaactga
     4381 cacaaaatat tttagaaagg aacgaatgat ctatggaaat gatacaaacc aaacgagtcg
     4441 cattgtatat tcgcgtgagt acacaagaac aagctttaga aggacatagt attcaagcac
     4501 aagaacaaaa tggacgacag tttgccgaac ggatgggcta tgaagtggta gaagtttatg
     4561 ccgatgaagg aatcagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     4621 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     4681 gcaatatgtt agatgtctta caaacagtcg aggaattatt gcgatatgac gtaggattac
     4741 attcgatctc ggaacaattt gacgtgacca cttcgacagg taaattgatg ttacaactcc
     4801 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcaa atgtcaatgc
     4861 aaagcttagt gaaagataaa aagcgttatg caggaggccg acgtttaggc tatgtcagtg
     4921 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     4981 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     5041 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     5101 tcttacgcaa taaaatttat gcagggtatt tagaatatgc gcgctacatc aattgggaaa
     5161 ccaaacgacg gaaggggaag aatccgagac cgattttagt gaaaggaacc catgaaccga
     5221 ttatttcgga agaagattat caaaaagtac aagaacgcct tgctttagaa ggaaaacaac
     5281 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     5341 gcgcaccgat ggcagcgagt aatgtcacaa ataccctaaa ggatggcaca aagaaacgga
     5401 ttcgatatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     5461 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     5521 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     5581 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     5641 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     5701 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     5761 ggatctttca acaaggagag ccgattcaag tcaaagatgt ccaacggata ttgactaatt
     5821 tggatcgttt tcttgcccag agtgagaaga agcaaatcaa agccttgtat cgtaccttta
     5881 tagagaaaat aacctttgat cctaaccata aggagatgtt ggaaattacg atgaaattca
     5941 cgcatgccgt ggtacaacaa ttgaatgagc aatatcaaat agccgtatcc aaaacaaagg
     6001 atacggctat ttttgtgtta aaaacaccct ttacgttaac gatctagtct aggttatatc
     6061 aaacaacgtt agaggggcgc taacgcttct ctagcgattt gaaatcgtta gagaagtagt
     6121 tagggaggtc cttatcataa atagggatat ttattaatga atatttgaaa tataattgta
     6181 tataagaatt aaaaagagtg gggatagata tgaaaaagat gggtaagtgt attttatgtg
     6241 aaaaatacac tgaacttact aaagaacatg taccacctaa aaaatcaggg aatactggag
     6301 gtaaaaagac cagaactggt aatttagatg atttccttaa gagcgatttt acaaaaggtg
     6361 attttccgaa ggggattaaa aggaaacctc aaggaaatgt ttattata
//
LOCUS       DANJJH010000118         6303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000118 DANJJH010000000
VERSION     DANJJH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            334..714
                     /locus_tag="PWS53_002539"
     CDS             334..714
                     /locus_tag="PWS53_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL0872725.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="PWS53_002540"
     CDS             696..1610
                     /locus_tag="PWS53_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0872726.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="PWS53_002541"
     CDS             1855..2544
                     /locus_tag="PWS53_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872727.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="PWS53_002542"
     CDS             complement(2665..2751)
                     /locus_tag="PWS53_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0872728.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="PWS53_002543"
     CDS             3427..4377
                     /locus_tag="PWS53_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL0872729.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="PWS53_002544"
     CDS             4398..4931
                     /locus_tag="PWS53_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0872730.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="PWS53_002545"
     CDS             4947..5117
                     /locus_tag="PWS53_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872731.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="PWS53_002546"
     CDS             5567..6100
                     /locus_tag="PWS53_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872732.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DANJJH010000119         6272 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000119 DANJJH010000000
VERSION     DANJJH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..925)
                     /locus_tag="PWS53_002547"
     CDS             complement(<1..925)
                     /locus_tag="PWS53_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0872733.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDY"
     gene            complement(927..2366)
                     /locus_tag="PWS53_002548"
     CDS             complement(927..2366)
                     /locus_tag="PWS53_002548"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL0872734.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2551..4497
                     /locus_tag="PWS53_002549"
     CDS             2551..4497
                     /locus_tag="PWS53_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL0872735.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4761..5633
                     /locus_tag="PWS53_002550"
     CDS             4761..5633
                     /locus_tag="PWS53_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0872736.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            5929..>6272
                     /locus_tag="PWS53_002551"
     CDS             5929..>6272
                     /locus_tag="PWS53_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0872737.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         1992 a         1098 c         1124 g         2058 t
ORIGIN      
        1 tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca acaataagac
       61 ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat ttctcaataa
      121 aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt ttaagttgat
      181 gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata ccatctggtt
      241 tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac agattattag
      301 gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca cgtaattctg
      361 tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa ccattttttt
      421 gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca atggaaacat
      481 taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca taatcagcaa
      541 ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca agcatttcaa
      601 tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt ttatatccat
      661 gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata ttactaatat
      721 taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt gctaaattat
      781 ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt ttttctgata
      841 aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca gctaattcag
      901 cgacatcagt taattttgct accattttaa tattttaatt caaagtattt tccagtaggt
      961 tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg actagtaaac
     1021 actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa aatgttagca
     1081 gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt ggcatattga
     1141 atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac aatcaagcta
     1201 agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt tggaggaaaa
     1261 gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg ttcagaacta
     1321 gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc ttttattgaa
     1381 agttctttta caagactcat tgctccctga taatcatact tatcagttgg ataatcgata
     1441 tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt gaatccttga
     1501 gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt
     1561 actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat attcccataa
     1621 tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt tttgtctaca
     1681 aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa atcaaggttg
     1741 ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt gataaagcct
     1801 gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata attaaacatt
     1861 tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac attctcacct
     1921 ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc acgttcccaa
     1981 ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat ttcatatgca
     2041 gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt
     2101 acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa cccatgagca
     2161 gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca
     2221 ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg ccaaggtgat
     2281 cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga ataagcacga
     2341 tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt ttacaactag
     2401 tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata aactatatga
     2461 caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag tgaaccgttt
     2521 tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc ttccgacgta
     2581 atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac acgtcttcgt
     2641 ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg
     2701 aaaagcacat ttaaaattaa tggacaatat caagtgatta tcggtgcagg tgatgttaat
     2761 ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac agatgattta
     2821 aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat taaattatta
     2881 tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt aatggcatta
     2941 caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga aatgtatcct
     3001 gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt catgttctta
     3061 ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt tcttggtgct
     3121 gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata ttggaatatt
     3181 tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt tttagcagct
     3241 gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc atctattgac
     3301 tttacattca cgccattatt atcagtaatg attactggat ttttgacttt cacagttatt
     3361 ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg gctatataac
     3421 actactggat ttattggaat gggtattttt ggaggaacat attcacttat agtcatgact
     3481 ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg gacaaatggt
     3541 atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct
     3601 gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct tgcttcctct
     3661 gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt taacttaaaa
     3721 taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc agtagcagga
     3781 ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt tctttcaatt
     3841 aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt agttgctttt
     3901 gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc agaagaaatt
     3961 gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat atctactgat
     4021 gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat ttctggtaaa
     4081 gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat gggaaaaggg
     4141 attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac tattcaactc
     4201 acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga agttctaatt
     4261 catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa agttactact
     4321 aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat gattgagaaa
     4381 gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta ttcagaagta
     4441 aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat caaataaata
     4501 tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag attaatcaaa
     4561 tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa aaattattag
     4621 aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt
     4681 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
     4741 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
     4801 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
     4861 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
     4921 cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
     4981 ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc ggtaccatga
     5041 aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct gcctatggtg
     5101 agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg atcggtacgg
     5161 gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt catccagaaa
     5221 tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc tgtccatttc
     5281 atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaacgc ttagggaaaa
     5341 aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc tatattgctc
     5401 aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt ggcggtggtg
     5461 tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg
     5521 ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta gaagacaacg
     5581 ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca taaacgaaaa
     5641 aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt agtcataacc
     5701 agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc ttgaatgaga
     5761 taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg tattgtgcaa
     5821 tagtttttta cacgccgttt gtataattaa gcgagacaaa taaaaaagcc atttatgtta
     5881 cactcaaatc gtaatacccg ctaaagcata caaggagagt gaacataaat gacttatacc
     5941 catcttacac caaacgagct tgtaatgata gaagcatatt ttcatcaaga aactccggtt
     6001 gctatcgttg cgaagcagct taaacgtgga cgccaaacaa tttacaatgt ctataacttt
     6061 ctcaaatgtg gtggaacagc acttgaatgc tttgaacaat acaaagaaaa taagcgacgt
     6121 tgtgggagaa ccgaaatcat ttttcctgct gaggaaaaag aatacattgc aaaaagatca
     6181 actgaaggtt ggaccccaga cgtcattatt ggccgtgcag agcgaacctt ctcttgttca
     6241 gtaagtaccc tctatcgccg gtttaagacg gg
//
LOCUS       DANJJH010000120         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000120 DANJJH010000000
VERSION     DANJJH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            725..811
                     /locus_tag="PWS53_002552"
     CDS             725..811
                     /locus_tag="PWS53_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0872738.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWS53_002553"
     CDS             complement(932..1621)
                     /locus_tag="PWS53_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872739.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWS53_002554"
     CDS             complement(1628..1906)
                     /locus_tag="PWS53_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872740.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWS53_002555"
     CDS             complement(1866..2780)
                     /locus_tag="PWS53_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0872741.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWS53_002556"
     CDS             complement(2762..3142)
                     /locus_tag="PWS53_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL0872742.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="PWS53_002557"
     CDS             complement(3592..3879)
                     /locus_tag="PWS53_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0872743.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="PWS53_002558"
     CDS             complement(3899..4123)
                     /locus_tag="PWS53_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL0872744.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="PWS53_002559"
     CDS             complement(4578..4748)
                     /locus_tag="PWS53_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872745.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="PWS53_002560"
     CDS             complement(4763..5296)
                     /locus_tag="PWS53_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0872746.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="PWS53_002561"
     CDS             complement(5317..6159)
                     /locus_tag="PWS53_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872747.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DANJJH010000121         6126 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000121 DANJJH010000000
VERSION     DANJJH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1256)
                     /locus_tag="PWS53_002562"
     CDS             complement(<1..1256)
                     /locus_tag="PWS53_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0872748.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="PWS53_002563"
     CDS             complement(1270..2487)
                     /locus_tag="PWS53_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0872749.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="PWS53_002564"
     CDS             complement(2477..4072)
                     /locus_tag="PWS53_002564"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL0872750.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="PWS53_002565"
     CDS             4197..4982
                     /locus_tag="PWS53_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HDL0872751.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            5691..6050
                     /locus_tag="PWS53_002566"
                     /pseudo
     CDS             5691..6050
                     /locus_tag="PWS53_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1861 a         1227 c         1030 g         2008 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
     5221 ggaaaatatg cttctatcat tacaagctcg tttggtgtaa gatgggtata agtcatttat
     5281 gttcactctc cttgtatgct ttagcgggta ttacaatttg agtgtaacat aaatggcttt
     5341 tttatttgtc tcgcttaatt atacaaacgg cgaattataa ataactcata aaaaatattt
     5401 tgactttcaa aatcactttt gctaccagaa gttgaggcga agtgttttaa gcatagttgt
     5461 accctagttt gaccgttaaa aataaataac gatcgctaga gtgcaactat gtttgtttgc
     5521 cctaccgtca gcgtcggctt ggcctctcac atccattacc gtcgggatta tctccgttac
     5581 atacccttac aaaatacgcc ctgtaagcat tgcatgaaac caccgtgcat ttgcaccgta
     5641 tttccgccct agccgttcaa atcggtcgct accaccattc agaaacacgt gcggtaatgg
     5701 aaggtttgga ggaagctatc cagaccacta acgattgtcc ctatcgtcgt acgtttcact
     5761 cagatcaagg ttggtcgtat caaatgaaag cttatagaaa caaattgaag gagtacaaaa
     5821 tctttcaaag catgtctcgg aaagggaact gcttagataa ttcacttatg gagaattttt
     5881 ttggcttatt aaaacaagag atttttcacg ggaaagttta taactgtttc gaggaattaa
     5941 aatcagccat cgatagctat atctattact acaataatga acggataaaa cagaaattaa
     6001 actggcaaag tcccgtccaa tttcgtaaaa caaccgttac ggttgtgtaa aaatatcatt
     6061 ttagagcatg aaaaaaagag tggatttctc cactccaaaa aagtctaact tttgggggtc
     6121 actaca
//
LOCUS       DANJJH010000122         6001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000122 DANJJH010000000
VERSION     DANJJH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..395
                     /locus_tag="PWS53_002567"
     CDS             <1..395
                     /locus_tag="PWS53_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872752.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="PWS53_002568"
     CDS             398..1228
                     /locus_tag="PWS53_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL0872753.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="PWS53_002569"
     CDS             1244..2095
                     /locus_tag="PWS53_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0872754.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="PWS53_002570"
     CDS             2092..2451
                     /locus_tag="PWS53_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL0872755.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="PWS53_002571"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="PWS53_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="PWS53_002572"
     CDS             2624..2929
                     /locus_tag="PWS53_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872756.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="PWS53_002573"
     CDS             2929..3240
                     /locus_tag="PWS53_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL0872757.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="PWS53_002574"
     CDS             3237..3410
                     /locus_tag="PWS53_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872758.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="PWS53_002575"
     CDS             3407..3823
                     /locus_tag="PWS53_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL0872759.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="PWS53_002576"
     CDS             3823..4491
                     /locus_tag="PWS53_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL0872760.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="PWS53_002577"
     CDS             4488..4865
                     /locus_tag="PWS53_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872761.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="PWS53_002578"
     CDS             4862..5161
                     /locus_tag="PWS53_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872762.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="PWS53_002579"
     CDS             5151..5309
                     /locus_tag="PWS53_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872763.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="PWS53_002580"
     CDS             5403..5819
                     /locus_tag="PWS53_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL0872764.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DANJJH010000123         5809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000123 DANJJH010000000
VERSION     DANJJH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            450..1604
                     /locus_tag="PWS53_002581"
     CDS             450..1604
                     /locus_tag="PWS53_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL0872765.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="PWS53_002582"
     CDS             1598..2020
                     /locus_tag="PWS53_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872766.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="PWS53_002583"
     CDS             3143..4075
                     /locus_tag="PWS53_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872767.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="PWS53_002584"
     CDS             4092..4607
                     /locus_tag="PWS53_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0872768.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="PWS53_002585"
     CDS             4903..5184
                     /locus_tag="PWS53_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872769.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="PWS53_002586"
     CDS             5186..5602
                     /locus_tag="PWS53_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872770.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DANJJH010000124         5790 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000124 DANJJH010000000
VERSION     DANJJH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(99..1229)
                     /locus_tag="PWS53_002587"
     CDS             complement(99..1229)
                     /locus_tag="PWS53_002587"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL0872771.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PWS53_002588"
     CDS             complement(1877..3079)
                     /locus_tag="PWS53_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0872772.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PWS53_002589"
     CDS             complement(3139..3342)
                     /locus_tag="PWS53_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL0872773.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PWS53_002590"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PWS53_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PWS53_002591"
     CDS             4879..5691
                     /locus_tag="PWS53_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872774.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DANJJH010000125         5582 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000125 DANJJH010000000
VERSION     DANJJH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(59..2008)
                     /locus_tag="PWS53_002592"
     CDS             complement(59..2008)
                     /locus_tag="PWS53_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0872775.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="PWS53_002593"
     CDS             complement(2050..3108)
                     /locus_tag="PWS53_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL0872776.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="PWS53_002594"
     CDS             complement(3227..3682)
                     /locus_tag="PWS53_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872777.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="PWS53_002595"
     CDS             complement(4266..5069)
                     /locus_tag="PWS53_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872778.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1647 a         1101 c          773 g         2061 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 ga
//
LOCUS       DANJJH010000126         5550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000126 DANJJH010000000
VERSION     DANJJH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            86..457
                     /locus_tag="PWS53_002596"
     CDS             86..457
                     /locus_tag="PWS53_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872779.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(741..1826)
                     /gene="xerS"
                     /locus_tag="PWS53_002597"
     CDS             complement(741..1826)
                     /gene="xerS"
                     /locus_tag="PWS53_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL0872780.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2160..2393
                     /locus_tag="PWS53_002598"
     CDS             2160..2393
                     /locus_tag="PWS53_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL0872781.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2390..2797
                     /locus_tag="PWS53_002599"
     CDS             2390..2797
                     /locus_tag="PWS53_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0872782.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(3934..4452)
                     /locus_tag="PWS53_002600"
     CDS             complement(3934..4452)
                     /locus_tag="PWS53_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL0872783.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1851 a          902 c          878 g         1919 t
ORIGIN      
        1 agaagaattt acaaaagtta tttcaacttt ttatattgat gatttgtacc aacattttgg
       61 ttttatttgt atgtggttac tttttatgac cgttatgcgt ttgggagaag ccactgcatt
      121 aacttggaaa gatattgatt ttgaaaataa aaacttgcgt gtgaataaaa cactctatta
      181 taagagagcc aaaacctatg agtttgtaga acccgaaacg gcttctagta ttcgtacaat
      241 cgaactagac gacaatacaa ttaccttttt aaaagcttgg aaggagcgtc aagtttcaat
      301 aggaggcatg tcgtttgtac tttcttataa tggaacacct actcagaaat atacaatccg
      361 tcatattatg agaagacatg caacgatcgt tggtgttcat ataatccgca tacacggatt
      421 aaggcatagt catgcttctt tgcttatatc acaataaaaa gatgcacttc gaattaaaga
      481 aaggttaaaa catgctgatg tacaaacgac tttaggaact tatggtcatt tgtatctaaa
      541 ttcattgaga gagataacaa atgatcccac agattatatc caaactaatt tttctaaaat
      601 caaaccgaca cctatttacg ataataaaaa ataaatgata aacaaaagta ggatcacacc
      661 aaaagaaaca cactagaaaa actataatac aggcttttct agcgtgttgt ttcttatttc
      721 cgcctattta tacattatac tcataaagca tttaatgcat cgatcgtagc cgcattgtct
      781 atatgggtat ataaagttgt tgtattggta cttgaatgtc ccaattgttg ggcagttaat
      841 accaatgaat ttgtctgttg gtagagtcta gtagctaatg tatgacgcag tttgtgaggg
      901 ctgacttgaa ttctaaagcc ttttgaatat tttgctacca tccgttcgat tgcaataccg
      961 gatatacgct tggcttctcc ttggtaaatt gctagaaata aagctttttc gttttctgtt
     1021 actttatatc gttctttccg aatagctaga taattagcta aatattccat tgcgaatgct
     1081 gctatattga cagcatctct tttatttcct tttctagtta ctaccacatt attcttatct
     1141 aaagataggt catctaaatt aatatttata acctcggaga gtctcaatcc agatcctaaa
     1201 atcagtgcat tgatcgccag gtctctttct acatctcgtt gataatagat caaagctcgt
     1261 ttactcaaag tatttttata ttcattggca atataatcta gataattttg tgtatcctta
     1321 tctagaaata attttttctc aatggcattg gcacgtgcag ctaacgtagt tttatctgtg
     1381 atggttgcta cttgatccat aggattattg tctaagtatg gttttcctag aggattattg
     1441 gaacttttcg aaagatattt aaatagaacc tttaatgccg tgatatttct attgatagta
     1501 gaagtagata gacctaagtt attattatct ttgagcttag tttttgttgt ctcttttttt
     1561 acattttctt ttgtgcgacg tttcaaatga gatttgtaca agtttaggtc ataggcagtt
     1621 aactgtctta tcgaggtgat agaagtatca ggaacgatag actcactcag aagccattca
     1681 taaaagagct tataattttt tagatattct aaagtggttc gaggtgattg atctttatcc
     1741 tcacgatctt gaacaaaatc ttttatataa tcgggcatat gaagtatgat tgtatctatt
     1801 ttttgatata tttctgtatt tttcatgtca ttcatccttt caataagtag gggagaagag
     1861 tatctctata attggactat accaattata gaatgttttg tcaattggta tgctctaaaa
     1921 tttattaatt tgtttcaaaa aaagcatata aacaggtata cgtttaataa tttaaaataa
     1981 atggtataca taaataggga aggaaataga agggagcaat ctaaattttt tgaattttct
     2041 taattaatta tatagaaaga acaatctttc tatagagtta gacatgagag ggactatgct
     2101 tgatttaaaa tcaacaagcg tatatactgt atatacaaag aaaaaggagg gagaagacta
     2161 tggcaattaa agaaagaaaa tttagggaag tggggaactc agtagtatta actatttcaa
     2221 aggagtttct taaagaaaat gggttaaatc ccggagatac ggttttttta gatgaagaaa
     2281 aacttcgagc ggcaattaca aaaaaacaag ccttatctga tgaaattgat ttagcaatat
     2341 ctcaagcatt aactgaatat gatactactt ttagagagct tgtcgataaa tgagtattca
     2401 ttacctagaa gccaaagata ttataaaaat gaatgtaatc caaataaaaa aatactctcc
     2461 taatgaaccc cttggtgtaa aggatgcgaa tgctttagaa atgtgtgtag ggcaacttcg
     2521 agcctcagct tttggtgaag aggtttatcc ttcgatttat gaaaaagcag ctatcctact
     2581 tatacaattg ataaagaaac atccttttca caatgcaaat aaacgtactg ctttcttagc
     2641 tacttttgtc tttttgaaaa tcaatggaca gcttttagct attgatcaaa aagatgctgt
     2701 aaatttagcg gtatatattg ctacgtatga taaggatttc gatcaactaa aatatgaagt
     2761 gatcaacact ataaagacac atacgcagcc catttaagga gttactgtat gtgtctttat
     2821 agggttatta gctgctatag cgaaataatt gaagtaaatt acaaatagct ataattcaaa
     2881 tccaactata tagaaaacgt aaattttcta tatagttgga ttaagataac gactatttat
     2941 tttatcatat ttttactata ccctatactt ttacttggta tatttatgaa taatcgcaaa
     3001 tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag ttatttgttc
     3061 ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac gaaagaataa
     3121 aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag tgagttagtg
     3181 cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct tgcagaaatc
     3241 ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt gaacttctac
     3301 tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc aaatcccgta
     3361 tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg ccacataccc
     3421 tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta gcatattaac
     3481 aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa tttcaattct
     3541 tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca tgtgggttgt
     3601 tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa
     3661 aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat atggcaccat
     3721 aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat
     3781 ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc caataaaaac
     3841 cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata caagaggtct
     3901 cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct tccattttct
     3961 tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa atttatatac
     4021 ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag
     4081 atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac gattgagaat
     4141 ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact tatataactc
     4201 tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc catttctttc
     4261 atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta aattttcagc
     4321 aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa tagtttcaaa
     4381 ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt ctttattttt
     4441 atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat tcatctataa
     4501 tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat tgactgtcta
     4561 actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa tgttgatcgt
     4621 cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga ctggtttgca
     4681 tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact aataaaaact
     4741 accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc acaaaatcca
     4801 ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt acagcatcgc
     4861 tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga cgatgacacg
     4921 ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact cgaagaaaat
     4981 ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat taaaggtcaa
     5041 gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc acaatgcatt
     5101 tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt aatgaaacag
     5161 atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat
     5221 cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt gtttaaggtt
     5281 ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt taagttggtt
     5341 gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg acagtaccta
     5401 aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg ctcatttcaa
     5461 cttatatgtt ttagtgaact acccccactt agcttggata ggctacaatt aactagacag
     5521 aaaaattaag gtgtgtagac tagaagaaaa
//
LOCUS       DANJJH010000127         5532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000127 DANJJH010000000
VERSION     DANJJH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..985)
                     /locus_tag="PWS53_002601"
     CDS             complement(<1..985)
                     /locus_tag="PWS53_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL0872784.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="PWS53_002602"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="PWS53_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0872785.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="PWS53_002603"
     CDS             1810..2964
                     /locus_tag="PWS53_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0872786.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="PWS53_002604"
     CDS             3179..4147
                     /locus_tag="PWS53_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0872787.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="PWS53_002605"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="PWS53_002605"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0872788.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="PWS53_002606"
     CDS             5177..>5532
                     /locus_tag="PWS53_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL0872789.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DANJJH010000128         5211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000128 DANJJH010000000
VERSION     DANJJH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            8..1015
                     /locus_tag="PWS53_002607"
     CDS             8..1015
                     /locus_tag="PWS53_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0872790.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..2031
                     /gene="neuB"
                     /locus_tag="PWS53_002608"
     CDS             1027..2031
                     /gene="neuB"
                     /locus_tag="PWS53_002608"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL0872791.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2028..3176
                     /gene="neuC"
                     /locus_tag="PWS53_002609"
     CDS             2028..3176
                     /gene="neuC"
                     /locus_tag="PWS53_002609"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL0872792.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3149..3826
                     /locus_tag="PWS53_002610"
     CDS             3149..3826
                     /locus_tag="PWS53_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0872793.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3816..4958
                     /locus_tag="PWS53_002611"
     CDS             3816..4958
                     /locus_tag="PWS53_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0872794.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4971..>5211
                     /locus_tag="PWS53_002612"
     CDS             4971..>5211
                     /locus_tag="PWS53_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0872795.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKE"
BASE COUNT         1932 a          676 c         1026 g         1577 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
     1261 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
     1321 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
     1381 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
     1441 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
     1501 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
     1561 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
     1621 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
     1681 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
     1741 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
     1801 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
     1861 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
     1921 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
     1981 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
     2041 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
     2101 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
     2161 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
     2221 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
     2281 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
     2341 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
     2401 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
     2461 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
     2521 cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat aatagattac
     2581 ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt
     2641 tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt
     2701 tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg
     2761 ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata
     2821 ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg
     2881 gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta ataaatttag
     2941 gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa
     3001 atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa
     3061 atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc
     3121 aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata
     3181 tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac
     3241 cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca
     3301 agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa
     3361 aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa
     3421 attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa
     3481 agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc
     3541 tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat
     3601 taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt
     3661 aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg
     3721 tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg
     3781 caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa
     3841 gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga
     3901 tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt
     3961 ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac
     4021 tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag
     4081 taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat
     4141 ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat
     4201 tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt
     4261 tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt
     4321 ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg
     4381 gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg
     4441 ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata
     4501 gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa
     4561 tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag
     4621 ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc
     4681 gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa
     4741 taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc
     4801 aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa
     4861 agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag
     4921 aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag
     4981 gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa gaacacttta
     5041 atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa gttgaaaagg
     5101 aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt gaaataaatt
     5161 cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag g
//
LOCUS       DANJJH010000129         5061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000129 DANJJH010000000
VERSION     DANJJH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(205..660)
                     /locus_tag="PWS53_002613"
     CDS             complement(205..660)
                     /locus_tag="PWS53_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872796.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="PWS53_002614"
     CDS             complement(1063..1236)
                     /locus_tag="PWS53_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872797.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="PWS53_002615"
     CDS             complement(1917..2456)
                     /locus_tag="PWS53_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0872798.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="PWS53_002616"
     CDS             complement(2472..3395)
                     /locus_tag="PWS53_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872799.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="PWS53_002617"
     CDS             complement(4523..4678)
                     /locus_tag="PWS53_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872800.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="PWS53_002618"
     CDS             complement(4697..>5061)
                     /locus_tag="PWS53_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0872801.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DANJJH010000130         4998 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000130 DANJJH010000000
VERSION     DANJJH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            262..1452
                     /locus_tag="PWS53_002619"
     CDS             262..1452
                     /locus_tag="PWS53_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL0872802.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1586..3775
                     /locus_tag="PWS53_002620"
     CDS             1586..3775
                     /locus_tag="PWS53_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0872803.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3772..>4998
                     /locus_tag="PWS53_002621"
     CDS             3772..>4998
                     /locus_tag="PWS53_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0872804.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1793 a          763 c          897 g         1545 t
ORIGIN      
        1 gcaatttttg cacctaatca tactctgttt atatatgaaa actagattct gaatccaata
       61 tttggttaac gagaggcgtt cgaatttgaa cgtcttttat atattacttt caaataaata
      121 tgaggtagaa tactatctat tttcttttta acccaattgt tataatggag agtgtaaaat
      181 attttgtgta aatagaaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt
      241 cacagaagag aggacttccc tatgaacgat tttactacag aaattctaaa gactctagcg
      301 aacaaaggcg atttgaatga attattccgt gtccatttgg agaaagcagt caatacgctt
      361 ctcaaaacgg agttaacggc tttcctagat tacgaaaagt atgatcgcat tggttttaac
      421 acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca agaccgagta tggggaactt
      481 catctccaga ttccgcgcga ccgcaatggt gagttcaagc aacagactgt tcctgcttat
      541 agacggacga atgacacgtt agaggagacc gtcattcacc tcttccgaaa aggtattacc
      601 atgtcggaaa tcgcagactt gattgagaaa atgtatgggc attactacac gccccaaacc
      661 atgtccaata taacaaaatc atttacagaa gaggtaacgg cgtttaatgg gcgggagctt
      721 catgaccgtt atgctgctat ttatatggac gcaacgtata ttccgttaaa gcggaaaacc
      781 gtcgccaagg aagctattca tatcgcagtt ggcattcgcc cggacggatc aaaggaagta
      841 ttgagctatg cgattgcacc gactgaatcc atcacgattt gggaggaaat tttattggac
      901 cttcaagagc gcggtttgaa aaatgtcctc ctgttcatca cggatggctt aaaggggatg
      961 gtaggagcga ttagtcggtt ctatcccaaa gctcgttttc aacattgttg tgtacacgtt
     1021 tcccgtaata tcagtcataa agtgcgtgtc gatgatcgta aggaagtctg tgatgatttt
     1081 aaaatggtgt atcaagcctc atctaaagag gtggcgttgg aagcacgtgg tacttttgcg
     1141 gaaaaatgga aaaccagcta tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg
     1201 ctcactttct atgatttccc cttggccata cgcaagagta tttattctac gaacttgatt
     1261 gaatccttta ataaacaaat caagaaatac agccaccgca aggaacagtt ccaaaatgaa
     1321 gagtcgatgg aacgtttctt agtctcgtct tttgatactt acaaccaaaa attcctaggt
     1381 cgcagtcata aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg
     1441 attgagaatt agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct
     1501 cccttataat gagaactttt actaacataa tttatgataa acttactata taatcatatt
     1561 aattaaatag ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag
     1621 aaattatgaa tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa
     1681 tgattcaggg aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta
     1741 ctatagtagt aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca
     1801 agggcattgg attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa
     1861 tgagatttgt gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat
     1921 ttcaaatgca gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat
     1981 tagaaagcaa ttatttgata tttccactga attagactct catcaattta acgaatttag
     2041 aaattctatt cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt
     2101 ctgtatcaat gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa
     2161 tcctgaagaa gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa
     2221 gcatatttcg gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa
     2281 tcgaaaccca gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt
     2341 tgagtcatta aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat
     2401 taggaaaatt ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc
     2461 tcctaatctt cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct
     2521 ttcaatgaag ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat
     2581 gatttattta gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa
     2641 tattatgttg attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt
     2701 cgaaggatta aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca
     2761 tttagctgaa gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc
     2821 tatcgttatg aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa
     2881 caatctaaat ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct
     2941 tttttctaaa ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat
     3001 agtaaaaaaa ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg
     3061 tagtacttcg tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg
     3121 tgctattgta actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag
     3181 cactgcacaa gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa
     3241 caattgggtt aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa
     3301 taaagaaaat attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa
     3361 aacaataact gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc
     3421 gatgttaaaa ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa
     3481 aattgacttt ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca
     3541 ggaaatagta gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga
     3601 ggaaactaca gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt
     3661 gaatatagca ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct
     3721 aatcaatcag atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat
     3781 atcttcgaaa aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa
     3841 gataaccttt atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa
     3901 attgcttacc aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac
     3961 actaatagag ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat
     4021 attaatattt gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt
     4081 aaaatgaatc tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga
     4141 gcttacgtca gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac
     4201 aaaattgatt tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt
     4261 tctgaaatgt atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta
     4321 ctagtagcgt ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgctagtaat
     4381 ataagggcga tttatgtgga tgaatttcaa gatactagag aattacaata caatgtaata
     4441 gctaaattac ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa
     4501 gcaatttatg gatcattaga tggtatagct aaatcagttg aagaactaga aatgctaact
     4561 agtcacactt ttcaatcaaa gacgctacat ggctgttaca ggtctaatca gagattagta
     4621 gacttctact ctaattttca gagttgtcct tacgagattg aaagcagagg gaataataaa
     4681 aacgaccgtg gttttataag ttatgattgc aaagttacta aggaggattt acccaacata
     4741 ataaaaaata taataaagga aaagattgaa agcggcattc atgaaaaaga tatttgtgta
     4801 cttgctcctc aacactatcc tctgttttca ctgggtgagt atttaaagaa agaattaccg
     4861 tattgcaatt ttgatgctat aaatatatca ccaattaagg taaataatca tagtttattt
     4921 ttcaagttag caattttgta ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta
     4981 attgcaaacg atatttta
//
LOCUS       DANJJH010000131         4856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000131 DANJJH010000000
VERSION     DANJJH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(406..780)
                     /locus_tag="PWS53_002622"
     CDS             complement(406..780)
                     /locus_tag="PWS53_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0872805.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="PWS53_002623"
     CDS             complement(803..2203)
                     /locus_tag="PWS53_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL0872806.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="PWS53_002624"
     CDS             complement(2207..2617)
                     /locus_tag="PWS53_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0872807.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="PWS53_002625"
     CDS             complement(2635..3126)
                     /locus_tag="PWS53_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0872808.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="PWS53_002626"
     CDS             complement(3136..3927)
                     /locus_tag="PWS53_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0872809.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="PWS53_002627"
     CDS             complement(3920..4693)
                     /locus_tag="PWS53_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0872810.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1543 a          992 c          643 g         1678 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaa
//
LOCUS       DANJJH010000132         4844 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000132 DANJJH010000000
VERSION     DANJJH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..290)
                     /locus_tag="PWS53_002628"
     CDS             complement(<1..290)
                     /locus_tag="PWS53_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0872811.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="PWS53_002629"
     CDS             complement(283..624)
                     /locus_tag="PWS53_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872812.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="PWS53_002630"
     CDS             complement(798..1523)
                     /locus_tag="PWS53_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0872813.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="PWS53_002631"
     CDS             1576..2052
                     /locus_tag="PWS53_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872814.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="PWS53_002632"
     CDS             complement(2058..2240)
                     /locus_tag="PWS53_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872815.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="PWS53_002633"
     CDS             complement(2240..2539)
                     /locus_tag="PWS53_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872816.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="PWS53_002634"
     CDS             complement(2644..2847)
                     /locus_tag="PWS53_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872817.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="PWS53_002635"
     CDS             complement(2862..3608)
                     /locus_tag="PWS53_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL0872818.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="PWS53_002636"
     CDS             3686..3994
                     /locus_tag="PWS53_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872819.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="PWS53_002637"
     CDS             complement(3965..4222)
                     /locus_tag="PWS53_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HDL0872820.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="PWS53_002638"
     CDS             complement(4520..4660)
                     /locus_tag="PWS53_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872821.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT         1329 a          938 c          758 g         1819 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct tttt
//
LOCUS       DANJJH010000133         4815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000133 DANJJH010000000
VERSION     DANJJH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..258)
                     /locus_tag="PWS53_002639"
     CDS             complement(<1..258)
                     /locus_tag="PWS53_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872822.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="PWS53_002640"
     CDS             complement(274..924)
                     /locus_tag="PWS53_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872823.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="PWS53_002641"
     CDS             complement(935..2107)
                     /locus_tag="PWS53_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0872824.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="PWS53_002642"
     CDS             complement(2126..2998)
                     /locus_tag="PWS53_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0872825.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="PWS53_002643"
     CDS             complement(3020..3940)
                     /locus_tag="PWS53_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872826.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="PWS53_002644"
     CDS             complement(3940..4209)
                     /locus_tag="PWS53_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872827.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="PWS53_002645"
     CDS             complement(4206..4586)
                     /locus_tag="PWS53_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL0872828.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1261 a         1057 c          792 g         1705 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctcca
//
LOCUS       DANJJH010000134         4788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000134 DANJJH010000000
VERSION     DANJJH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            564..989
                     /locus_tag="PWS53_002646"
     CDS             564..989
                     /locus_tag="PWS53_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872829.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="PWS53_002647"
     CDS             1019..1183
                     /locus_tag="PWS53_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872830.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="PWS53_002648"
     CDS             2141..3118
                     /locus_tag="PWS53_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872831.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="PWS53_002649"
     CDS             3128..3625
                     /locus_tag="PWS53_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872832.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="PWS53_002650"
     CDS             4001..4282
                     /locus_tag="PWS53_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872833.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="PWS53_002651"
     CDS             4284..4700
                     /locus_tag="PWS53_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872834.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa atcagcgcct ggatcccttg
      361 gtacaggcgt tacttggtag tatacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DANJJH010000135         4689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000135 DANJJH010000000
VERSION     DANJJH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            229..573
                     /locus_tag="PWS53_002652"
     CDS             229..573
                     /locus_tag="PWS53_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL0872835.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            574..993
                     /locus_tag="PWS53_002653"
     CDS             574..993
                     /locus_tag="PWS53_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL0872836.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1085..1435)
                     /locus_tag="PWS53_002654"
     CDS             complement(1085..1435)
                     /locus_tag="PWS53_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872837.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1401..2621)
                     /locus_tag="PWS53_002655"
     CDS             complement(1401..2621)
                     /locus_tag="PWS53_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL0872838.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2987..3553
                     /locus_tag="PWS53_002656"
     CDS             2987..3553
                     /locus_tag="PWS53_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0872839.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3698..3785
                     /locus_tag="PWS53_002657"
     tRNA            3698..3785
                     /locus_tag="PWS53_002657"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3730..3732,aa:Ser,seq:cga)
     gene            4119..>4689
                     /locus_tag="PWS53_002658"
     CDS             4119..>4689
                     /locus_tag="PWS53_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL0872840.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1700 a          786 c          901 g         1302 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtaaaata
       61 aaaaaagcgt tagcaactat gcttaaaaag catatcgact gaataaacta agcattggac
      121 aggctgaaag actacattta aaatgaaatg cgaagaaaca atagaaaata aagttgcctt
      181 agaaccatag ttgaatcacc taacagtcgt gaagggagaa taagagaaat ggcagaaaaa
      241 caaaccgcag gacgagatca attaggagat tttgcaccaa aatttgcaga attgaatgat
      301 gatgttttat ttggacaagt atggtccaga gaagaattat ctgctcatca gcgtagtatc
      361 atcaccatat ctgcattaat ttcaggagga aattttgaac aattgccagc tcatctgaag
      421 attggcaagc aaaatggcat tactagtgaa gaaatagcag aaatcattac ccatttagct
      481 ttttatgtag gatggccaaa agcttggtca gcttttagtt tagcaaaaga aatctattta
      541 gaagaaaata gcgaagataa gaaagaggcg taaatgacga caaaacacga agaaatacac
      601 aatggcgtga tttttccagc aggtgaaaaa aacgaagctt acgctcaagt atttaccggg
      661 caaagctatc tgaatatgtt agtgaatgat cccgaagttt ctgttggtgt aggtaatgta
      721 atttttgaac caggttgtcg caatcactgg catgtccata aagacggcta tcaaatctta
      781 cttgtgaccg gaggagaagg ctggtatcag gaagaaggaa agccagcaag acgattaatg
      841 gctggagatg tcgtcgtaac gcacaaaggg attaaacatt ggcatggtgc gacaaaagac
      901 agttggtttg aacatttggc gatcactgct ggtaattcag agtttttaga acctgtaaca
      961 gatgaagtgt atgaaggatt ggaaaatcaa taatgtcaag cttgaacagc tcctgaaagt
     1021 agattttaat cgaactttca ggagctgctt ttttgattca gtcttttata aatagaaaat
     1081 agggttaata ataggaatga gaagcacgtc gaaagatctt aatattgata atcggctggg
     1141 catacatgat ttccacgtca tcaatcaaac tacctaacag atgataatct tcccgatcat
     1201 gtcggggatt acctaatagt tcttgatagt agatttccat actagggtcc cgaggattgt
     1261 tcagcaaatc agcaagtaca tctaaatctt ctggctgagt ttctgccgta ccctgtatct
     1321 ctatgaaaag cccttcctca gcaaattcta ctccaatacg tatttcagtt atttcatggg
     1381 aatataaata agtaagaagc tcatcgatga ttctttttga taattctttt tccaagtgtc
     1441 tttttttcct ttctgacagc ggtaaagtta tctagtatcg gtacaaggat acctgcaacg
     1501 aaaccagttg aaaaaccact gttatataga tttagtcctc cgtgcaaagt gctgacgtta
     1561 tggacaagag caatatgtag tacaccagct attacaccgt aaaaactgcc gtaaaaaccg
     1621 cttatgggcg cgagacctgt cccaaacaca gctgcaacaa gcatgccagt actggtgatc
     1681 tcatgaaaag ttccaaacaa agaagcaata aatattccga ctagtatcgg aaaactgttt
     1741 tttaaatgac aaccaaaagc actgaaaccc accgcagata aaatagcacc gatgacaggt
     1801 ccatttaact gaccgccaac cagcaaaaca taagaagaca gcatcagacc aactaagcct
     1861 atgttgatca aagtagcacc aataccagaa ttagcaatga aatcagttgt caattttcca
     1921 gatgaatcga aaatttctcg aatccccaaa agccgaaagg aattataata gaaaccaatt
     1981 ccgactgtca gcgaaaaaaa gccaaaaata atccaaatga gaggaaagtg atattcattg
     2041 aaaaccaatg tattttcgac gattgaatag ctgaataacc tcaaaacagc agtaaaagtc
     2101 atcgcaatca acccagaggt gaatccaata ttataaagtg tgaatccacg atggaaaact
     2161 aaagtttgag cagctaaggg cggaagaagt agaccaattg ctatgccagc taaattcccc
     2221 aataaaatac ctatcagtaa aggaaaacgc ataccaaaag tgatataact aacgaccggt
     2281 gataatgcgc taccaaacag tccgatcatg atgtactgag aaaaaggttt attgacaaat
     2341 acagcgtata gatacacacc taaaatgatt gagatagaat tataaaggtt tttgccaaaa
     2401 aaagagaaac cggtaactgt tagaatcgaa gcaaataagg gaccgtttaa aatcaccttg
     2461 tgccgataag ctaaaagcac agaaagtaag gtcaaaatcc ctacatttat gaatgcgctt
     2521 ccgaaaccgc ctaaagcaaa ataatctgtc aaaagattgc ttggcgaggt aagaatagtt
     2581 agcaatccac tccaattatc agtaggagta ttagaaagca agccaataaa aagaaaaaaa
     2641 cacctgtaaa accaagataa aaatactttc ttttctgtga aacgattcgt ttttgaggat
     2701 gaatataaat aacgtcagtt tcgttttcca ttacacacct tctttttcat tttctttaac
     2761 tataacttga aatgaaagat ttggacaact atcatttagg tcaaaactaa aaatgtaagg
     2821 gaacccattg aatttaacca aattttttta attttgcaaa aaaaagttaa agaagactga
     2881 aaaaaaattt aaaatataac gttttgagta aaatgtttgc aaaaaatgct aaaaataggg
     2941 tacaataaaa caacatatac tgcagtaaag aagtggggga gttttaatga gaagaaaagt
     3001 ctataccaag gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa
     3061 aggatttact gcgagaaata ttgctaaaaa gatggggatt tcgacacagc cgatttatct
     3121 ggaattcaaa aacatggaag atttaaaaaa tacgttgctg gattctatct ttgaacaact
     3181 aaaagaagat gttttcccta ttgaacgtac tggtgataaa atcattgatc tcggtttgaa
     3241 ctatatctac tttgcaaaaa agcatcatag tctttacatg gcattgtaca ttgatgaaca
     3301 tggggacgga aagaaaatgc atgatttttc atacaattat ttccgtgaaa tagttaaaga
     3361 agatgaaaga tacaatcaat tatcggatga atatgtcgat gctctgcata ctggtacatg
     3421 ggtagcagtg actggcttag cgtcattgat gtcatcaaac atcatgcatc caacagaaga
     3481 acaaatcatt gcactgatca aacggacaat tgactcgatt ttagcattag ataatcctga
     3541 aataatcaga tagtgttcta tcagtatgaa tttgatcggt aagtccgttg gctgcaaact
     3601 gtcaatggac ttttttgcta aaaaaagcga tttctgttat ttatctatta gaaataggct
     3661 tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga aggttggcag agtggtaatg
     3721 caccggactc gaaatccggc gaaccggcta ataccggcgc gcaggttcaa atcctgtacc
     3781 ttccttatat caacgattag aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg
     3841 gccaaacaaa aatagctttt cctatttttt cgtttgattg ggcttattac cgcagatgca
     3901 agcttttgaa caccgtttgt tcggaataag gggctgtgac aagacttttg tcacagcccc
     3961 ttattgttgt acttttaacc tacaatattt tttgattccc cctgagtact attttttata
     4021 aatgctagta aatcaatctt ctttggcctt tttttgataa aaaaaatact agatgtaaga
     4081 taaaagtgta aaaacgattt gttttaggag gggaaacgat gtgtaccgca atcacttatg
     4141 caacgaaaga ccattatttt ggaaggaatt ttgattatga gatgtcttat aatgaagtgg
     4201 tcactattac tccaagaaat tatcgtttcg attttagaaa ggtaaaaaat ttagataaac
     4261 attatgcaat gataggaata gcagctgggg tatccaacta tccactctat tatgaggcta
     4321 caaatgaaaa agggttgagt atggcaggac tgaattttcc tggcaatgct gattataaag
     4381 aattacaaga aggaaaagat aacgttgcac catttgagtt tattccatgg atactagggc
     4441 aatgctcaac catagacgaa gctaaagaat tattggctac tatcaatcta gtcaatatcg
     4501 attttagtga aaaactaccc ttgtcacctt tacactggtt attagctgat aaagaaaaat
     4561 cgatagtaat tgaaagtatg aaagatggac ttcatttata tgataatcct gtcggtgtgt
     4621 tgacaaataa tccgccattt gattatcaat tatttaatct gaacaattat cgctcgctat
     4681 caaatggaa
//
LOCUS       DANJJH010000136         4637 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000136 DANJJH010000000
VERSION     DANJJH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            636..1547
                     /locus_tag="PWS53_002659"
     CDS             636..1547
                     /locus_tag="PWS53_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HDL0872841.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2034..2300
                     /locus_tag="PWS53_002660"
     CDS             2034..2300
                     /locus_tag="PWS53_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872842.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2372..2713
                     /locus_tag="PWS53_002661"
     CDS             2372..2713
                     /locus_tag="PWS53_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872843.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2768..3187
                     /gene="ssb"
                     /locus_tag="PWS53_002662"
     CDS             2768..3187
                     /gene="ssb"
                     /locus_tag="PWS53_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0872844.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3264..3560
                     /locus_tag="PWS53_002663"
     CDS             3264..3560
                     /locus_tag="PWS53_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872845.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3575..4006
                     /locus_tag="PWS53_002664"
     CDS             3575..4006
                     /locus_tag="PWS53_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0872846.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4188..4493
                     /locus_tag="PWS53_002665"
     CDS             4188..4493
                     /locus_tag="PWS53_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872847.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1701 a          730 c          989 g         1217 t
ORIGIN      
        1 tggggggagg gctgtcggcg cttggtatga aaggcattct ccgacttcca ggtttttgac
       61 cattttctct caacgtggaa aaaccgccac ttatgagaga aaatggtcaa aaacctaagc
      121 gggaagtctc cgaataacat accaagcgcc gacagccaac agataaagaa tacactacta
      181 aaaatagtag tagcataaaa tttaaaaggg aaaaaatcaa cccctacccc agtcttgggg
      241 tctcaaaact aactcccccc taaccccctc tttcagcctc ttttgaaaga gggggagaat
      301 caaccccacg gccaacgccc cagtgttctt ggggctgtcc agaacagaaa cttgcgagag
      361 agtgggggta aaacaagtgt agaggaaact tgaagacttg ttcgaacaga aacttgcgag
      421 agagccggag taaaacaagt gtagaggaaa cttgaagact tgttcgaaca gaaacttgcg
      481 agagagttgg agtaaaacaa gtgtagagga aacttgaaga cttgttcgaa cacaaactta
      541 caagagaggg gagtaaagca gttgcagagg aatcaggaag atttgttcga acagaaactt
      601 gcaagaaagc attattaaat tgaaggtgat gaaatatgaa gtatttattg catggctatt
      661 ttattgatta ttctactcgc aatagtagta ctttagcgat tctatgtttg cttcatgaat
      721 tgagagtggt tactagaaaa cagctataca aactattgaa ttttgacaga tcaattagta
      781 aacaaggagt aacgaatgct ttagtgagat tatatgaaaa acagttaatc gatcgaaaaa
      841 aatatggaac agaaatgtat tattatttga cgaaagaggg acatcaaagc ataggtgggt
      901 attatacgtt gccgaaagtt cctgaatata atttgaatca ccatttacaa attaacaatt
      961 atttgattaa aggaatggca ttggcaaaag accacccaca tttaaaacgg attatttcag
     1021 aaagacgtac cgtctatgaa acgaaggatt taaatcctaa gacaaaagga agaaaatttt
     1081 ttgtgccaga ttttattttt cgttttcgag acaaagaaaa tcatgagatc aattggtcct
     1141 ttgaaattga gctaacgttg aaaacacgtc gtcgctaccg tgaagcgatt tttccgaaat
     1201 atattcgtga gttgcgaaca cgtcctgaat cacatttaat ttatgtgaca cctagtggcg
     1261 caattcaaga agaacttcat gcgtttaagc gtattttttt caagcgcgaa actgaaaaag
     1321 atgcgagtca acaagaattg tttgagcggt tgcatatttt ttcagcttta gagtttgaag
     1381 agggattaaa agcattatta gagaatgatc cagcagtcaa ttggcggaca gtagaacgag
     1441 aaaaaattta tccgaatggc gctgttcaaa gtcgtttgaa tttatttgaa gaatcaactg
     1501 aaaatgagga aaaaccaaaa gtgaagttta cgcctgaagt attgtaaaag agggtgttcg
     1561 cttcgctctt tttttgtgcc agtttgctgg caccgttcag acgcaagcgt ctgaaccggc
     1621 tcgtttttag gaaggactaa cttgtttgat ttatccgttg tcaaagaagt ggacgttcga
     1681 gagtccctaa tcgtatagca gtcattcggc gagtataacc taaaacataa cctgccaatt
     1741 gccaagggta aaatgcacgc tgcgcgccct tggcaatcgt cagaacatgt tttcgagccg
     1801 gttatgccga acgaccgcta tacgaaaagg gcctctaaac agaagtccac tttgatcctt
     1861 gacaactgaa acccaaaatc aaacaagcag gccttcgtct aaaaacgagc cgaatgaaga
     1921 ccttgggggt tgcaccccca aacccccgtg cccccgacac actaccgaaa aggtgggggt
     1981 gggtggcgaa aaaatcaaat aaaacattct attcaagaaa ggaagaaaca accatgacaa
     2041 ctaaaaacga gcaactgaaa gaacaggcaa gaaaagagtt gcaagaaaga ggtttgatta
     2101 ttgaggggag ctttgaagga gattttgaaa cctatattgg ttgttatgca agaccgataa
     2161 acaaacctac agccttagac ccaatgaacg aacaagaagt aaaagaacaa gaaaaacatg
     2221 cagtgaatgg ttttccgcaa gatttcactg aatggtatga gtgggaaatt aacaacggga
     2281 agcttgaaaa cttcttataa aagtcagatg acgtgcaaag agaatgattc tctttgtacg
     2341 gtaaaaatat aaagaaagaa ggaaaaagaa aatggcaaaa aacacacctt atcaagtagg
     2401 agacattttt tatagtagtt ggggctacga acaaacgaac gtgacttttt ggcaaattgt
     2461 gaagttaaca gaaaaaacag catggtttca gcctattcaa aagaaaacgg tcgagcatta
     2521 ttcgtccatg agtggaaaag ttgttccaat gaaagacaag tttatagagt atcctttagc
     2581 aaaaactaaa gacagtattg taaaaaaacg aattgatcca gaaacctatc ctgatatgtt
     2641 atgtggttat cgtagttggg acacgtttca tagatataaa aatcaagctg tttatgaaag
     2701 tagttattat taaggggata gtttcccctt tcaaaaataa taaatagaag aatggagaga
     2761 aaaaatcatg atgaattcag tgaatttagt aggacggtta acaaaagaag tggatttaaa
     2821 atacacacca aaaggcaatg cgacaggcac atttatttta gctgttaatc gaaattatac
     2881 gaatgcaaac ggcgagcgtg aagcagatta tatccgctgt gtcatttggc ggaaagctgc
     2941 ggaaacttta gcaaaatata ctggaaaagg aagtttgatt ggcattaatg gacgtcttca
     3001 aacgaggagc tatcagaatc agcaaggaca gaccatgtat gttacggaag ttctagtgac
     3061 agatttctat ttgttagaga gtaaagaaat caatgagcaa cggtcaaaat caatggcgaa
     3121 taatcaaaat aattcttcaa ttccaggaaa tgagattcaa atttcagata gttatttgcc
     3181 cttttaagtg aaaaataatt ttgttctata cctgatttct tcaggtatag aacaaaaaat
     3241 aaatagagat gaggcgattt tttatgaaaa tcattgacag ttacgatcaa cgaaccatgc
     3301 aatatttggg ctcgtttgat tatacagatg aaaatattat taattatgtt gcaagtttac
     3361 ctccttatca aagcgttcgc ttggtagatt ttgcaagtga tgaattggta ttaacaacga
     3421 tgggcaactt tttagattat gtcggagaca aaaaatggtt ggaaaaaatt ttgtctaaat
     3481 tgattgcgaa acaaacagga gaagaaccca taaatgcgat tcaattgttc aatcgatatg
     3541 aacaagaaaa caatgattaa agaggagaga aaaaatgaaa agattaaaac gaagtatttt
     3601 tattgtgcta gcattgattg attttgttgg gacaggttgt gaagcaattg acgttcaaaa
     3661 agctccctta tcggaaataa aacaagcaat aacaaaacaa gatgaagaac aaccgctaaa
     3721 tacacaagta gaaatagtga atcaaaataa accgagtttt tctgaagaag atttttcgtt
     3781 agaaaatgga accaggcaat ttttttcaga tttggatcgt ttaaatcggg taggtgtagc
     3841 aagtgcactt ttgcataaaa gcaggatgcc aacagaagaa cgagaccgat tatacattaa
     3901 gccaactggc tggaaacaaa agaaaatgaa gaatggcgat tatctttata atcgttgtca
     3961 tttgattggc tatcaacgtg cgacaagttt ccccataagt atgtaatacg aaacgggagg
     4021 attaatctta agattaatca caactaacct atttgacagt tggaatgaaa gctatcatgt
     4081 ctgctgaatc aactggtctg atactcctac atgcaataaa gtctactagg aagttattgt
     4141 atgaagctag gtaaaggcgg cagaacaatt tctaagtgtg gaatcatatg aagttggata
     4201 tgccgggacg ctataaatta ctcagggtga gactgttttt aattaaactg acgaacttgc
     4261 gaatgtacgg gtctataacc gacttatatt cgaaagatat aagattgcga atgcaaagaa
     4321 cattatcgaa aagtaagaat gttggtcatg aaattcgaga tggaagacta gcggtgtatc
     4381 tgtcaagagt tggtaaaaca tgtttgattg attcagatga gagtgggaat gcgacaaaac
     4441 gttttacaga agaaatagag caactagcag aactgaatat acgatatttt taaatacttt
     4501 agcggagaat aaaaaaatcc tctacagaaa actacggagg gcaacatgtg ctattgtttc
     4561 aacaatacat ttttaatttt taccataaca aaaaagtact tgaaaaaaaa cagaaatatg
     4621 ttaaattaaa aatgaaa
//
LOCUS       DANJJH010000137         4636 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000137 DANJJH010000000
VERSION     DANJJH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1383
                     /locus_tag="PWS53_002666"
     CDS             <1..1383
                     /locus_tag="PWS53_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872848.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
     gene            1397..1546
                     /locus_tag="PWS53_002667"
     CDS             1397..1546
                     /locus_tag="PWS53_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872849.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1543..1989
                     /locus_tag="PWS53_002668"
     CDS             1543..1989
                     /locus_tag="PWS53_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872850.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1991..2128
                     /locus_tag="PWS53_002669"
     CDS             1991..2128
                     /locus_tag="PWS53_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0872851.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            2165..2458
                     /locus_tag="PWS53_002670"
     CDS             2165..2458
                     /locus_tag="PWS53_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872852.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2455..2679
                     /locus_tag="PWS53_002671"
     CDS             2455..2679
                     /locus_tag="PWS53_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0872853.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2676..3695
                     /locus_tag="PWS53_002672"
     CDS             2676..3695
                     /locus_tag="PWS53_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL0872854.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3884..3957
                     /locus_tag="PWS53_002673"
     tRNA            3884..3957
                     /locus_tag="PWS53_002673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3918..3920,aa:Ile,seq:cat)
BASE COUNT         1605 a          806 c          933 g         1292 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taacttacta
      481 gatgaccctt actgggtagg taaaacatcg ttgggtgaga atattgcaaa taaaggtacg
      541 acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga agaagagctt
      601 ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      661 gtagcatata attattggaa cgttaatttt ggagaactaa aaccagttga gggattgaca
      721 gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc taaagacctt
      781 cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc aaattgactg gctcaaaatt
      841 gaaaaaggcg acacccgaac cccgaatatt agtcagttta aatactttgg tgaaggattg
      901 aaagatagca acaatccgaa cgactacagc tgggacatca cgcctgaata tactgaaaaa
      961 ggcttgaatg atacggttag tttgactgaa ccgcaatcca tcgaaggatt gaaaaatttc
     1021 gaagatggta ttcaatcaaa aggaaaatct gtattgacat cagacgacaa caaatatgaa
     1081 gtcgtaacct taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
     1141 aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca
     1201 acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc
     1261 tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt
     1321 aaatggcgaa gaaactcaag ttatggatca gcttattcat ttgttatcac ttattcaatt
     1381 taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca
     1441 aatcattgtg aatgacgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
     1501 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
     1561 tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct tgtatccgtt
     1621 ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc gctaacgatt
     1681 gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa cgcgttagat
     1741 cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga aaacggcaac
     1801 ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca ggaaaatact
     1861 gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat gcttgcttcg
     1921 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaagga
     1981 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
     2041 tgacgatcaa gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat
     2101 cattaataaa gataagagcg catcttaatt gatgcgtttt tatttaaggt aaaggagttg
     2161 tcacatgatt aatttagggg aatggggaat gatagcagga tcaataaccg ctatcgtttc
     2221 tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta ctgagactct
     2281 ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
     2341 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
     2401 aaaattggtg gaacacacgc aacaaatcaa aactttgttt agggagagaa gaaaatgaat
     2461 aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc actagctact
     2521 ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg tgttgtcatc
     2581 attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg aacctacaaa
     2641 atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt actattactg
     2701 cgatgagcct gttaacggct ctttttttat tgccaattaa cggatttgcc tatactatca
     2761 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtata
     2821 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
     2881 aacgttcatg gactagcgct tatactgctt atattgtggg agacggcgga attgtttacc
     2941 aagtcggaca acctggttat gtacagtacg gtgctggttc atatgctaat gccaatagtc
     3001 ctgtgcagat tgagttacaa cacacacatg ataaagcaac ttttgaaaaa aactataaag
     3061 catacgttga attggctaga gattcagcaa tgaaatatgg tattccatta acattagaca
     3121 cgccttataa ccaaccagga atcaaatcac atttatgggt aactcaaaac atttggggcg
     3181 atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa aaactggctt
     3241 acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac aagcctgtca
     3301 ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg aaccattctc
     3361 acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg
     3421 ctaactataa gtacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
     3481 gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat acttctggaa
     3541 atgttggcta tcatgtatct tttaacatgc gtaacttccc taataagaaa gtctatgtca
     3601 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
     3661 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcatt
     3721 acataactat attgacaact ataaaaatca ttcgataaaa tagtgatgtt atcgcatatc
     3781 ttcactatca cccataatag tcacactcca agctatgcga taacaggttt gttgccacac
     3841 attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt
     3901 ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacatta
     3961 acgtagccat ttgaatcgtt ctgtgttaga atttttttga agagtattat acaagctaaa
     4021 gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta tggattaatg
     4081 aaaggatgtt tcacatagtt atacttctgt atatttgaaa agttttactt tgatttttaa
     4141 atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta aagcgcaccc
     4201 caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga agccattaaa
     4261 aagcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga gtggtagtta
     4321 attagtgact tatttttgat tttatagcac tgatactata aaatatagat atcatcatat
     4381 tacacaatct taatactaac ttaaaaaata tctcctttca caagtatggt gataaaattc
     4441 gttccgggct acctttttag gtagcctact ttaatcttta tacctttctg gatcaacgaa
     4501 agtatacttt atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcat
     4561 gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata aaaaacacac
     4621 tggaggattt ttcatg
//
LOCUS       DANJJH010000138         4543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000138 DANJJH010000000
VERSION     DANJJH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1329
                     /locus_tag="PWS53_002674"
     CDS             <1..1329
                     /locus_tag="PWS53_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0872855.1"
                     /translation="YTSVLSTTEYGTYDLTISTATLLFPILTLNIVDAVMRFLMDKDK
                     NKKNIASIAIKVIFLSVIVFGSVMYLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQ
                     FAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVL
                     VRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLS
                     VAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIIL
                     TRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGAL
                     VNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFV
                     QAIIEIYFKNYVIEIIIMIILIIINISYIKQLCYRGRNIISMIKSREHKR"
     gene            1365..2081
                     /locus_tag="PWS53_002675"
     CDS             1365..2081
                     /locus_tag="PWS53_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0872856.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2100..3683
                     /locus_tag="PWS53_002676"
     CDS             2100..3683
                     /locus_tag="PWS53_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL0872857.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3818..4396
                     /locus_tag="PWS53_002677"
     CDS             3818..4396
                     /locus_tag="PWS53_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872858.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT         1660 a          511 c          837 g         1535 t
ORIGIN      
        1 tatacaagtg tattatccac aacagaatat ggcacatatg atttgacaat ttcaacagcc
       61 acattattgt ttccaatttt aaccttaaac attgtagatg ctgtgatgcg ctttttaatg
      121 gataaagata aaaataaaaa aaatatagca tctatagcga ttaaagttat atttttaagc
      181 gtgatagtct ttggatccgt aatgtatttg ttaaatatta caaaattatg gcaagatgtt
      241 tatggattag aaaaatatat ttttttgtat tatatatcct atgtatggaa tcaatttcta
      301 atacaatttg ctaaagggtt agaaaaagtt aaagctatgg gcatagcagg tattatcagc
      361 acaattgtaa caataattgc caatattttc ttcttgctta ttctgaaatg gggattaatt
      421 ggttttttca ttgcaaatat tttatcacag attatttcag ctacttatct gtcagtatta
      481 gtgagaatat gggaatatat cgattttgag gctaaagatc ctcagctaaa gagagaaatg
      541 ctaaattact cgattccatt aattgcaact gttgttggat ggtggattaa tagtacagct
      601 gataggtatg tggtttcctt tatagtaggt gtctcggcga atggcttatt gtctgtggca
      661 tataaaatac ctcaaataat taatactttg cagggaatat ttatacaggc ctggcaaatt
      721 tctgcaatta aagaatatgg agataatgag actgcaaaat tttatggaaa tacatttgtt
      781 atgattaatt tgttaatgtg cataacctgt tcaagtctta tcatattaac tagaccgttt
      841 tcacatattc tttatgcgaa agacttttat gaagcgtgga aatatgttcc atttttatta
      901 atagctaatg ttttaaattg tgcatcaggt ctgttaggac ctatcttatc agcaaaaaaa
      961 gattctaaat ccatgatgtg gtctgcaatc ataggggcat tagttaatgt gattatgagt
     1021 gtagtccttg tttataagat aggggttcaa ggtgccacaa tagccactgt tatatgttca
     1081 tatgtaatat atgttgttcg taaacgagct gtgggaaaag atatttttat aaagcattat
     1141 catattgttg tggaatcttg gggattattg tttgttcagg caatcataga aatatatttt
     1201 aaaaattatg taatagaaat tataattatg attattttaa ttattataaa tatcagttat
     1261 attaagcagt tgtgttatag aggaagaaac attatttcaa tgattaagag tcgagagcac
     1321 aaacggtaga cgatgttatt tttataaaaa gatataattt attaatggtc gataattatg
     1381 aaaggaaaaa tattcttaaa aaaaagagta taggatgtcg tatgaaaata gtcgctatag
     1441 taccaatgaa gttaaataac aaaagattgc cacaaaaaaa cacgaaagct tttaccaatg
     1501 gaaaaccatt gtgttattat atactttcaa cacttttaaa agtaaaacag attgataaag
     1561 tgtatgtcta ttgtagtaac ccagatatta aagaatttat accagaaggt gtaacatttt
     1621 taaagagatc tagttcctta gatcaggata caacgagtat tacacaggta ttagccggtt
     1681 tcacaaaaga agtagaggct gatgtatatg taatgacaca cacaacagca ccatttatta
     1741 gcgaaaaatc tattgaaaaa ggactagatg ccgtgctgac aggagaatat gattctgcat
     1801 ttgctgcaaa aaaattgcaa gatttcttat ggaaagacgg tgaaccattt aattatgaat
     1861 tggacaatat tcctagaaca caggatttgc cgcctttata cgaagaaact agtggatttt
     1921 atatttataa tcgcaatgtg atgaccgagt tgcatagaag aattgggaaa aaaccttgca
     1981 tagttgaagt aggtgagatt gagtcagttg atattgatga agccgaggat tttatgattg
     2041 ctgatgcgat ttataatcat cttattttac aaggtagtta agacaggggg aaggttagga
     2101 tgaacaatgt acagatttta gattgcacac ttagagatgg gggctattgt aacaattggg
     2161 aatttggatt tgataataca aaaaaaatta tttctggact tatagaatca ggaattgaga
     2221 ttatagaatg tgggtttctc acaaataaaa cagtatacaa taaaaatgtt acaaaatata
     2281 cgaccttaga agaattagca cagataattc ctgaaaaaag agaaggaaaa ttatttgtag
     2341 caatgatgaa ctatggcgaa tataatatag aggatcttcc agattatgat ggttcatcta
     2401 ttgatggaat taggattgcg tttcataaaa aaaattgcaa tgaagcgttg aaattatgtc
     2461 gtgagataaa gaaaaaaggc tacttagttt ttgttcaagc tatggtatct ctcaattata
     2521 cagatgaaga gtttttagca gtaataaaaa atgtgaatca gtttaaacca tacgcttttt
     2581 atattgttga tagttttgga atgatgaaag gaaaagattt aactcgtctt ttttacatga
     2641 ttgagcataa tctagaaaaa gatatacaga taggttttca ctcacataat aacatgcagc
     2701 ttgcatattc taatgcacaa aaacttataa ccgtacaaac aaatcgaagc ctgattattg
     2761 attcttcggt gtacggaatg ggaagaggag caggtaatct taacacagaa ctatttgtag
     2821 aatatttgaa tgataatgca ggaaaaaaat atcaattaaa accattatta tatataattg
     2881 atgaaatact aaatagtttc tatcagaaga gttattgggg atattcatta ccaaactata
     2941 tttcagcagt tcataatgct catcctaatt atgcaagcta tctgtcatat aagaatactt
     3001 taacaattga agcaatgaat gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt
     3061 atgataaaca atatatagaa gatttatact acaaatatat ggaaactggg aaaatacagg
     3121 aggaatatat ggaagaactg agaaaatttc tctcagacaa aaaaattcta ctgattgcac
     3181 caggaaaaag ttctgtagat gaggtagaaa gaataaaaga gtttgttacc aaagaagaag
     3241 tcgtaataat tagtgtaaat tttgaatatt ctcatataga tatagattac gtatttttaa
     3301 gtaatttaag acgttttcat gaattaccga aagaaagcag aactaaatgt attgttacat
     3361 caaatataca agcatcggat gtatatttac ggacgagtta taaaaagttg ttatctgaaa
     3421 gggaagttgt aagagacaat gcaggattaa tggcaattag atttttagcc gatatgaatg
     3481 tggatgaaat attcttagct ggatttgatg gatattcaca taatgaagat gagaattatg
     3541 gtgagcaatc catggaaatt ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa
     3601 tgagagatga attgtcagaa ttagctaaaa aagtaaaggt gacattttta acggatccac
     3661 gttgggttcg aattgataag tagtagtaat aatgttttta aaattttgat aaagagatag
     3721 tgagagaata aaaatgggta aagtcttaaa taaaaaagaa gaaaaatatt taagggacga
     3781 tggggcgaga tgaaactcta attttgaact tttaagaatc atttctatat tgtgtattgt
     3841 tgcttaccat gctaatttgc gtttaaatta taatgcagac ggaattatag tacatggttc
     3901 attaactgct aaccagattt tcttgaatta tattggtatt tggggtcagc taggagttaa
     3961 ttgttttata tttatttcgg catatttttt aagtgagaaa agaaacattc actttgaaaa
     4021 tgttttggga attgtattag aatgtatttt ttatagtgta ttagtttatg gaattctttg
     4081 tgcatgtgga atagaaacat tttcattgaa cagctttgta aaagaaatat tatcatttgc
     4141 tctaggtaga tattggttta ttacatccta tttagcattg tatattctaa gtccgtattt
     4201 aaatatcatg cttaacaatc tggaaaaact tcagttgcaa aaaataagta tgttattgtt
     4261 tataggtgta tgctgttatc gtatgttatt taaaacatct ttgtttgggg attgtgctgt
     4321 tttaattgca ctatatgtca tatgttatac actgaaaaaa acgataataa tatttttgag
     4381 agacacagaa tcataggaac aattgtatgt ataacagtta taccacttct catatccata
     4441 ggaatacttt tcaaatttat accaaatgca atggttaacc agatggttca tattggttct
     4501 ccattgatgg ttatagcaag tatattttta ttttatgtat tta
//
LOCUS       DANJJH010000139         4475 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000139 DANJJH010000000
VERSION     DANJJH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            323..1054
                     /locus_tag="PWS53_002678"
     CDS             323..1054
                     /locus_tag="PWS53_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0872859.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="PWS53_002679"
     CDS             1199..3226
                     /locus_tag="PWS53_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0872860.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="PWS53_002680"
     CDS             3334..4431
                     /locus_tag="PWS53_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0872861.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DANJJH010000140         4466 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000140 DANJJH010000000
VERSION     DANJJH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..563
                     /locus_tag="PWS53_002681"
     CDS             <1..563
                     /locus_tag="PWS53_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL0872862.1"
                     /translation="GDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENE
                     LAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANY
                     FSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFS
                     RVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            580..1014
                     /locus_tag="PWS53_002682"
     CDS             580..1014
                     /locus_tag="PWS53_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872863.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1631..>4466
                     /gene="esp"
                     /locus_tag="PWS53_002683"
     CDS             1631..>4466
                     /gene="esp"
                     /locus_tag="PWS53_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL0872864.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGE"
BASE COUNT         1644 a          647 c          873 g         1302 t
ORIGIN      
        1 gtggagatac gattcggtct gaaaaaaatt attctatcat tgtttttgaa aagttagcac
       61 aaacgtctat aacattagga atggatataa tagaagcgta tcaaagtaga gatgctttga
      121 tacaagaaaa cgaattagct gtatcgttac ctgaggtctt aaaagtgagg gattctggga
      181 ttgtttatta tactaaggaa atcggaaaaa ctaagataga gcatctttct cccttaattt
      241 cttctgtcgt tcaattcatc ggcttaaata tctataaaag aattactgta aaggaaatag
      301 ctaattattt ttctgtaagt gaaactaaat taagagaaag ctttaagaat gaaatgcata
      361 ttacgattta taattatatc tctaagcgaa aaatttctac ggctaaaata atgttgaaat
      421 caaaccatac tatcagtgaa gtttctttag gtttaggatt ttcagattct tcacactttt
      481 cgagagtatt caaaaagtac gcaggtgtat ctcctaagca atatcaatta ggtttagtgg
      541 acaatttcaa caacattagc tgagaggttt tattttatta tgagactata tcatttacgt
      601 agtttggagg agttgaggaa attggatttt aacaaaaaat ttttttggga aatttctgat
      661 ttgagttgtt tcgtagaatc tgagcgtcta agtaatggta ataatagtgt gttaaatgaa
      721 tatgacaaag tagtaattaa taaagtaaaa agtttatgtg aagaagctag agaatctatc
      781 agtcatatta atggtagcaa ttttaaacaa acattattta atctgattaa gctagatgct
      841 aaaatatcaa gttatttatt ctttttgata catgatgaat ttatggatta tcaaaaattg
      901 gatcagttaa ttgaaagaga aagctgggaa gattattacg tgggtctaac tttttcagaa
      961 aagttaaaca actacaagtt aatagattat aagtatttga gtgaaagcaa ttaattattc
     1021 atgtagagta ctagacactt taaatatgga caattcaatt ataaagaaaa agaatcaata
     1081 atttaatgag gctgtgacaa atgtcatttt ttgtttcgct aaaaacgagt gaaaaaatgt
     1141 tgataactcc aatcattcgt ttaagattct aggaatcatt cagaaatgct gtgaaaccga
     1201 tactttataa ttataaagaa ggtgatttca aattccgaag aaggaatttg aagtcacctt
     1261 tttacttatg tcacagtctc attcagtaat gcgggataag gctccttatt ccgttatttg
     1321 aatactctga agtttaatct aagaaagggt aatattttga aaacatgctt catcgcatag
     1381 aaatgaactt aatttcagaa agaatacata catatctttt tatatgtttt cagctgctga
     1441 atggttaaaa agcacaaata tccgaaaaaa acatgaagaa atgtactttt tttgttgtaa
     1501 tgttcatgaa attctctaag taaattagtt aacataaggt tagtaagatt cctattctgt
     1561 gaataatacg tatcagattt atcttttatt cttactagga aagtaattat taagggagtt
     1621 gatttgaata atggttagca agaataataa gagagtattt ctagaaaaaa caaagaaacg
     1681 agtattgaag tacagtatca aaaaattaag tgttggggtt gcatctgttt tgattggtgt
     1741 tggtcttgtc ttgggaacaa ctgaactagt taaagcacaa gatgaaattt ctccaagcag
     1801 cccacttgaa acagcgataa gctccgtaca agtaggtgac aaagtagctt cggggaatac
     1861 ttttcaggag aatcctggtt atacgaaaaa ttataacttt tcggatttac aatttagtcc
     1921 tcaagaacta actggagaca cactaaaagg caatacaatt ggatttgaag tttacggaaa
     1981 acataatatt gcagcatcaa ctaaaaactg ggaaatccgt cttcaattag atgaacgatt
     2041 agctaaatat gtagaaaaaa ttcaggtcga tccaaagaaa ggaattggat cttctagacg
     2101 gacctttgta agaattaatg attcgctggg tagacctaca aatatttgga aggtcaatta
     2161 tattcgtgca agtgatggat tatttgctgg ggcagaaaca accgatacgc aaaccgctcc
     2221 taatggggta attacgtttg aaaagagcct agatgaaatt tttaaagaga taggcataga
     2281 caatctcaag accgatcgtt taatgtatcg aatctacttg gtaagtcatc aagacgatga
     2341 taaaattgta ccgggtattg atagtacggg ctacttttta acagattcag atgatttcta
     2401 taatagctta gatgtatctg aaaataatcc tgaccagttt aaacatggat cagtaagtgc
     2461 aaaatatgag aaacctaata ctcaaacaaa agatggttca ggatccacag gtgctaatgg
     2521 agctataatc ttagatcata agttaacgaa aaattataat ttctcatatt catcttccgc
     2581 aaagggcacg ccttggtatg ctaattacaa aattgatgaa cgattagttc catatgtagc
     2641 gggtatacag atgcacatgg ttcaggctga caaagtaaca tatgatgttt cgtttgaatc
     2701 cggaacaaaa gtagctgatt tagcaatcga gcgacgggaa ggtcatgaaa attatggcat
     2761 aggttcaata actaataatg atttaactaa acttatcgat tttgctaatg caagtccacg
     2821 tccagtcgtt attcgatatg ttttacaatt gactaagcca ttagatgaaa tcttagaaga
     2881 tatgaaagca acagcacaag ttgaagaaaa taaaccattt ggtgaagatt tcatctttga
     2941 ttcttggttg tcggatacga ataaaaaatt aatccagaac acttatggaa caggttatta
     3001 ttatttgcaa gatattgatg gtgatggaaa ccctgacgat aaagaagaga gcggagacac
     3061 gaatccatat atcgggaaac ctgaattaga agaagtatat gatgttgaca caacagttaa
     3121 ggggaaagta ttcatccacg agttagcggg aacaggtcac aaagcccaac ttgttgataa
     3181 agaaggtact gtattagcag aaaaaactat cgctccaaat gaaaaagatg gggctccaat
     3241 ttcagatact gtagaatttg aatttacggg tgtagattca agtaaattaa tcgcgaaaga
     3301 tgaattaaaa atccaaatcg tttctccagg ttttgataaa ccagaagaag gttcaaccgt
     3361 tattaaggaa tcaccaaaag cggttgataa acaaaccgtg gtagttggat ttaaaccaga
     3421 tgctaaagaa tcaattcgga ataataaaaa cttacctgaa gatgcagagt attcatggaa
     3481 aacagagcct gatacttcta acgttactga tagtacgaaa ggtattgtaa ctgttaagat
     3541 cggaaatcga actttcgacg tggatgtaga gtttgctgta aaagcttctc aagctatgga
     3601 aaatgatgca acatacgtac ctataacaac aactccagaa acgacagttc aaagtggaaa
     3661 acctacattt gataaaccag atgttccttt agctaaagat gccttttcaa tttcagatgt
     3721 ctataataag tacttcggta atgcaagtgt tgatgcaaac actggtattg ttacattcac
     3781 tccagctaaa ggtgtaggag aatcggagcc gattactgga acaattccta ttaaaattgt
     3841 ttaccaagat ggttctgtag gcacgaccga tttagcagta actgtaagta aagatattta
     3901 tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca ccgcagggga
     3961 aggaacaagt attgaaagtg gaacaacagt ctttgcagtg aaagacggcg taagcttgcc
     4021 agaagataaa cttccagtgt tgaaagcaaa agatggttat acagatgcga aatggccaga
     4081 agaagcaact caaccaatta cagcagatga tacagaattt gtatcaagtg caacaaaact
     4141 ggatgatatc attgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
     4201 caccgcagga gaaggaacaa gtattgaaag tggaacaaca gtctttgcag tgaaagacgg
     4261 cgtaagcttg ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc
     4321 gaaatggcca gaagaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag
     4381 tgcaacaaaa ttggatgata tcattgaaaa cccaggggat aacattccgg ctggctatca
     4441 taaagtaacc tttactgcag gagaag
//
LOCUS       DANJJH010000141         4433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000141 DANJJH010000000
VERSION     DANJJH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(543..2048)
                     /locus_tag="PWS53_002684"
     CDS             complement(543..2048)
                     /locus_tag="PWS53_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0872865.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PWS53_002685"
     CDS             complement(2045..2731)
                     /locus_tag="PWS53_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0872866.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWS53_002686"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWS53_002686"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL0872867.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DANJJH010000142         4425 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000142 DANJJH010000000
VERSION     DANJJH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            690..1658
                     /locus_tag="PWS53_002687"
     CDS             690..1658
                     /locus_tag="PWS53_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0872868.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PWS53_002688"
     CDS             1669..2484
                     /locus_tag="PWS53_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL0872869.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PWS53_002689"
     CDS             2552..3262
                     /locus_tag="PWS53_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0872870.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PWS53_002690"
     CDS             3298..>4425
                     /locus_tag="PWS53_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL0872871.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DANJJH010000143         4396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000143 DANJJH010000000
VERSION     DANJJH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..93
                     /locus_tag="PWS53_002691"
     CDS             <1..93
                     /locus_tag="PWS53_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0872872.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="PWS53_002692"
     CDS             complement(151..1371)
                     /locus_tag="PWS53_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL0872873.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="PWS53_002693"
     CDS             complement(1391..2002)
                     /locus_tag="PWS53_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0872874.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="PWS53_002694"
     CDS             complement(2045..2977)
                     /locus_tag="PWS53_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL0872875.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="PWS53_002695"
     CDS             complement(3398..4258)
                     /locus_tag="PWS53_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0872876.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DANJJH010000144         4345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000144 DANJJH010000000
VERSION     DANJJH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(387..1853)
                     /locus_tag="PWS53_002696"
     CDS             complement(387..1853)
                     /locus_tag="PWS53_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0872877.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="PWS53_002697"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="PWS53_002697"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0872878.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DANJJH010000145         4324 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000145 DANJJH010000000
VERSION     DANJJH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..281)
                     /locus_tag="PWS53_002698"
     CDS             complement(<1..281)
                     /locus_tag="PWS53_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0872879.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="PWS53_002699"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="PWS53_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="PWS53_002700"
     CDS             complement(2359..2721)
                     /locus_tag="PWS53_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872880.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="PWS53_002701"
     CDS             complement(2725..3063)
                     /locus_tag="PWS53_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872881.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="PWS53_002702"
     CDS             complement(3042..3461)
                     /locus_tag="PWS53_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872882.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="PWS53_002703"
     CDS             complement(3454..3687)
                     /locus_tag="PWS53_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872883.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="PWS53_002704"
     CDS             complement(3684..>4324)
                     /locus_tag="PWS53_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0872884.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DANJJH010000146         4226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000146 DANJJH010000000
VERSION     DANJJH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..573
                     /locus_tag="PWS53_002705"
     CDS             <1..573
                     /locus_tag="PWS53_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HDL0872885.1"
                     /translation="ISKNQTSILELLDGYNSDLISEYKNEIETCVNEIRKLNWDIVSV
                     KKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPK
                     NLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELN
                     KSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            576..1472
                     /locus_tag="PWS53_002706"
     CDS             576..1472
                     /locus_tag="PWS53_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0872886.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1522..1911
                     /locus_tag="PWS53_002707"
     CDS             1522..1911
                     /locus_tag="PWS53_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0872887.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1898..>4226
                     /locus_tag="PWS53_002708"
     CDS             1898..>4226
                     /locus_tag="PWS53_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0872888.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1452 a          653 c          884 g         1237 t
ORIGIN      
        1 atttcaaaaa atcaaactag tatactagaa ttactagatg gatataactc tgatttgatt
       61 tcagagtata aaaatgagat tgaaacatgt gtaaacgaaa taagaaaatt aaattgggat
      121 attgtttctg tgaaaaaaga aatacaagat aaggcgaagg tagagcttga aattgaagat
      181 ttaaaaaaac aagtagaaaa gctatctcat aagagttata aagatgttta tgatgagtat
      241 tcaaaagaga cagatattta tagggaactg aaacgtgatg tggaggcact taaggaaatt
      301 cccaaaaatc taactgatag tgttgacaaa attgactttg gcaatggtat aaaagttaca
      361 gacgaaattg atgaacaccg cggagaattg attaagaatt tagtgaccaa tcgggctaag
      421 attcaaacaa taattaatga aatgtcagat gaaattgata gttatagagc agagctgaat
      481 aaatcagatt ggaaagttaa ctataatgag gtttcaaaga ggtatcaaag aggtatcaaa
      541 aacttagtga agagttatct caagatgaat taaaaatgtt acaaaacctt gattctttat
      601 atcaatcttt atcaaaaact gaagagcgat tggaagaaat tattcaacaa gaaataaagc
      661 ttaaagatta ccaagttgag gttgactcag caatgtcaag aatgaataaa tcatatcaaa
      721 atctctattc tgagagaagt agttcctcgc gcgaaatttt ttctgatatt acaggaatag
      781 aggctactat tcaaccaaat agggatttca ataattatgt taaagagctc agagcttgta
      841 ttaataagga ggacactttt gacagtgagt ttggatctct tattgataag ctgacaaaaa
      901 attccattac taatcaagat atatataatg acatacttga tataagagtt agtggggaaa
      961 gtagtatttt ttcagataaa aaattaatca attctataag aaaattgaat gatgcacaag
     1021 ttcttgaatt acaaaccctt aagccaaatg atttagtagt tatccgactt gaactaaatg
     1081 gaaaaaaagt tagtttatcg aatgctagtg ctggtcaaaa gacatctgca attctttcta
     1141 tgattttggc gtatggaaat tcaccactca tattggatca gcctgaagat gatttggata
     1201 gtcaattaat taataactta atcgtaagaa gtatcattac aaaaaaggaa aacagacaaa
     1261 ttttaattgt aactcataat gcaaatatcc cagttaatgg tgattcagaa tggttgatta
     1321 gcatgggaga ttcaaaagaa atatcggctg atcagtgcgg gtcggtagat gaacccaata
     1381 tcaagccaag aatatgtact gtgatggaag gtggcgagga tgcttttaca aatagagcga
     1441 aacgctatgg ttttaaacaa attgtagaat aacactagag ggtgagacat attattatgt
     1501 ccgccctttt taggaggaaa aatgaaaaaa ataaaaagct ataccagtat ctgggcagtt
     1561 gaaaaagtca tttacgcaat caatgatttt cagttgcctt ttcctgtgac ttttaaccaa
     1621 atggcttggt tcgttttatc tttactgttt gtcatcgtat ttgcccatgt gcctccacta
     1681 tctatgatag aggaggtctt tttaaaatac ttaggcatac cggtggctgt cacttggttt
     1741 atgtcgcaaa agacgtttga tggcaagaaa ccactgggat ttttacgatc ctttatcagc
     1801 tatcacttgc gatttaaggt gacttttgca ggaaagaaag tcaaagaaca gaagaaacga
     1861 tgggatgaac ccagtactct tgtaaggagt gtgaactatg tatccgatta agtatattga
     1921 aaataatctc gtctttaacc aagaaggcga gtgttttgcc tactatgaat tagtgcctta
     1981 caattactcc tttttgtcac ccgaacagaa atttcaagtg catgataact ttcgccaact
     2041 aattgcgcaa aatcgtgaag gcaagatcca tgcgttacaa attgccacag aaagtagtat
     2101 tcgcgccaca caagaacgat caaaaaaaga aattaccggt agactcaaag aagtagcgaa
     2161 acaacgaatt gatatacaga cagatgcatt agtatccatg attggcgata gtcagattga
     2221 ttatcgcttt tttattggct ttaaactaat tgcgacagat gaagaagtca atctaaagag
     2281 tctcaagaaa tctttctttt ctgggcttca agaatttgtc tatggagtga atcatcattt
     2341 gatgggcgat tttgtttcct tatccaatga agaaattcgc tgctattcta agcttgaaaa
     2401 attaatggaa agcaaattgg ctcgaagatt taaagtcagg cgagtcacac cgagtgacct
     2461 gacgtatttg attgaacata tttatggcga aaaagggata ccgtttgaag aatacgaatt
     2521 tcagctacca aagaaaaagc taaaatcaga aacactggtg aaacgttatg acttattacg
     2581 tccaaatcgt tgtttaattg aagaaaaacc aaggtgctta cggatggaac atgagaatca
     2641 tgagtcgtac gtagcgtatt taacaattaa tacaattgtg ggggagatgg aatttccttc
     2701 ctcagaactt ttctattacc agcaacaaca atttacattc ccaatcgaca caagtatgaa
     2761 tgtagagatt gtgacgaata aaaaggcttt agctaccgtt cgtaataaga aaaaagaact
     2821 gaaagattta gacaatcatg cttaccaatc tgacaatgaa acgaattcca atgtcttaga
     2881 tgctttggat tccgtggatg aattggaaac caccttggat cagtcaaaag aatctatgta
     2941 caaactgagt tatgtggttc gagtgagtgc cgaatcagtg gatgaactaa agcgacgctg
     3001 tgatgaagtg ctcgattttt atgatgatac caatgtaaaa ctagtccggc cctttggcga
     3061 tatgatgggg ctacatgaag aattcttacc ctcaagtaaa cgatatatga acgactatat
     3121 tcagtatgtt acgtctgatt ttttggcggg gcttggtttt ggggctactc agatgctagg
     3181 agaattagaa gggatttact ttggctacaa tgtggataca ggtcgtaatg tttacctgaa
     3241 accggcattg gcttcacaag gagtcaaggg ttcagtaacc aatgcgctgg ccgccgcttt
     3301 cctgggttct cttggtggag gtaaatcctt tagtaacaat ctcttagtct actatgctgt
     3361 tttgttcggt ggtcaagctg tgattgtcga tccaaaaggg gagcgaggag ggtggaaaga
     3421 aaccttgcca gaaattgcgg atgaaatcag tatcctcaac ttgacgagta agtcagaaaa
     3481 tcaaggtctg cttgatcctt atgtgattat gaagaaaaag aaagactcgg aaagtttagc
     3541 aattgatatt ctgacttttt taacaggaat ttcaagtcga gacggggaga agtttcctgt
     3601 gttacgtaaa gccattcgaa cggtgactca aagtgatcaa cgggggttat tacaagttat
     3661 taacgaattg cgaaaagacg ggtcaccaat cgcggaaaat atcgccgatc atatcgagtc
     3721 ctttaccgat tatgattttg ctcatttgct tttctcagac ggctcgatta cccaatcgat
     3781 tagtttagat aaacagctaa atatcattca agtggcggat ttggttttac cggatgcaga
     3841 aacgactttt gaagaatata ccaccatgga attgcttagt gtttcgatgc taatcgtcat
     3901 ttccactttt gccttggact ttatccatag taaccgagaa atcttcaaaa ttgtcgattt
     3961 ggatgaggct tggagttttc ttcaagtggc tcaaggaaaa acgttatcga ataagcttgt
     4021 tcgtgctggt cgggccatga atgctggggt ctatttcgtc actcaaaata gcgacgattt
     4081 attagatgaa cgtttaaaaa ataatatcgg gttgaagttt gcttttcgca gtaccgatat
     4141 tcatgaaatc aagaaaacct tggaattctt tggtttggat caggaggatc aaagcaatca
     4201 aaaacgcctg cgagatttgg aaaacg
//
LOCUS       DANJJH010000147         4148 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000147 DANJJH010000000
VERSION     DANJJH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1288)
                     /locus_tag="PWS53_002709"
     CDS             complement(<1..1288)
                     /locus_tag="PWS53_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872889.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHD"
     gene            complement(1299..1967)
                     /locus_tag="PWS53_002710"
     CDS             complement(1299..1967)
                     /locus_tag="PWS53_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0872890.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2097..2681)
                     /locus_tag="PWS53_002711"
     CDS             complement(2097..2681)
                     /locus_tag="PWS53_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0872891.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3012..3245)
                     /locus_tag="PWS53_002712"
     CDS             complement(3012..3245)
                     /locus_tag="PWS53_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL0872892.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3260..>4148)
                     /locus_tag="PWS53_002713"
     CDS             complement(3260..>4148)
                     /locus_tag="PWS53_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0872893.1"
                     /translation="GACSINSKDKDKVASNEKLKVVVTNSILADITENIAKDKIDLHS
                     IVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGGNAWFTKLVKNANKEENKDYF
                     AASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNIEKQLAEKDPDNKKFYKENLD
                     KYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGT
                     PDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIAEKGQDGD
                     SYYAMMKWNLDKIAEGLSK"
BASE COUNT         1358 a          734 c          525 g         1531 t
ORIGIN      
        1 gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa
       61 tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag
      121 tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa
      181 acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat
      241 ctttactaac tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt
      301 ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta
      361 tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca
      421 caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt
      481 ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga
      541 tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt
      601 cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt
      661 gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt
      721 tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg
      781 aagacggttc atccaatata attaatttag agtttagcga taaactacat ccaatagaaa
      841 ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat
      901 ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt
      961 tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat
     1021 cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt
     1081 tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat
     1141 tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac
     1201 tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg
     1261 aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa
     1321 aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa
     1381 gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca
     1441 gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc
     1501 tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca
     1561 gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt
     1621 aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac
     1681 catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat
     1741 atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa
     1801 taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta
     1861 gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga
     1921 aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca
     1981 aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag
     2041 tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt ttttaattaa
     2101 ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt taatagcata
     2161 gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa
     2221 atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc
     2281 tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat tttttcaaca
     2341 atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat acgtttattc
     2401 tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct
     2461 atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata catattaata
     2521 agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt
     2581 ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat cttagtttgc
     2641 tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg gtgagttata
     2701 atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac tttattatct
     2761 tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc cttagagcta
     2821 aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga attaatagac
     2881 tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc
     2941 agctattagg ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa
     3001 aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca
     3061 gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag catctcactt
     3121 acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt
     3181 ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt tttaatttgt
     3241 accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt
     3301 tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa
     3361 tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt ctatcatcca
     3421 cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt
     3481 ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca
     3541 cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat
     3601 cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa
     3661 tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact
     3721 gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa gcatgaggat
     3781 cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg
     3841 caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta aaccaagcat
     3901 ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt
     3961 gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt
     4021 gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc gaattagtaa
     4081 ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta tttattgaac
     4141 atgccccg
//
LOCUS       DANJJH010000148         4028 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000148 DANJJH010000000
VERSION     DANJJH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..299
                     /locus_tag="PWS53_002714"
     CDS             <1..299
                     /locus_tag="PWS53_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0872894.1"
                     /translation="ITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLL
                     RKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(477..2474)
                     /gene="tkt"
                     /locus_tag="PWS53_002715"
     CDS             complement(477..2474)
                     /gene="tkt"
                     /locus_tag="PWS53_002715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL0872895.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2576..2827)
                     /locus_tag="PWS53_002716"
     CDS             complement(2576..2827)
                     /locus_tag="PWS53_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL0872896.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2974..3597
                     /gene="lexA"
                     /locus_tag="PWS53_002717"
     CDS             2974..3597
                     /gene="lexA"
                     /locus_tag="PWS53_002717"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL0872897.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3777..3995
                     /locus_tag="PWS53_002718"
     CDS             3777..3995
                     /locus_tag="PWS53_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872898.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1141 a          857 c          738 g         1292 t
ORIGIN      
        1 caattacctt tgattgtgga aaagaattct ctaattggaa aagtattagt aatacgaatg
       61 atattgatat ttatttcgca gacccaggaa cgccttccca acggggatta aatgaacatt
      121 caaacggact cctccgaaaa gatggactac caaaagaaat ggacttcaac caagtcaatc
      181 aaggattcat ctcatccgtt gcgtccaaaa gaaaccatat ccctagaaaa tcactaaatt
      241 accaaacacc attagaagtt tttttgagtt acgtaaatgg aaagttttgt ctcgcttaat
      301 ttgacaaata atattctcaa gaaacacaaa ctaatccgaa gaattctttg ttttgtcatt
      361 taaatgtaac atagccgaaa acgaaaagta atattgattt attcttctaa cacataaaga
      421 acagctgtct ccaacttgca gacagctgtt cttatcagtc aaacaattgc ttggatctat
      481 gatagttgat tgtacgtatt cacgacattt tctactgtaa atccaaattc ttctaaaatt
      541 ttagtgccag gggcagatgc accgaaatga tcgattgtaa tcgttgcgcc ctctgttcct
      601 acgtagcgtt cccatccaaa tgatgcagca gcttcgatag ccacacgttt cttcactgat
      661 ttaggaagta cactttcttt atattcagaa gattgttttt caaagaggtc aaagcttggc
      721 atagaaacaa cagaaacatc tttgcctttt tcagccaatt ctttttgagc tttcacagcc
      781 aaatcaactt cagaacctgt tgcaatcaaa ataccttctg gtgtttctcc ttgtgatgga
      841 gaaagaacat acgcgccttt tttcaccatt tcatctgcta cttcttttgt agaaggcaat
      901 actggaaggt tttgacgact caatacaagg atagtaggcg catctgttgt ctccatcgct
      961 actttccaag cagcacgtgt ttcatttccg tctgccggac gaatcacttg aacaccaggc
     1021 atgcagcgaa cagatgctag ttgttcaatc ggttcgtgcg ttggtccgtc ttctccgaca
     1081 gcaaccgagt catgtgttaa gacgtaaaca acaggagtat gttggattgc tgctaaacga
     1141 actgctggtc ttagataatc gacaaagaca aagaatgttc caccgtagat acgagttccg
     1201 ccatgaagtt ggataccatt catagccgaa gccattgcaa attcgcggac accgaaccag
     1261 atgttgcgtc cttcataatg ttcaggagtg aaatctttat ctgcagccac cattgtatta
     1321 ttggaacctg acaagtccgc tgaaccaccc cagaagcttg gtatagcttt cgataattct
     1381 tggatgactt ctttacttga aacacggctt gcttgactgc ttccaacttc ataagttggc
     1441 aattctgcat cccagttttc tggtagtttg ccatcaaaag catcttcaaa ttgttgtgct
     1501 aattcaggat aagctttttt ataattggca aacatttcat tccatgcatc ttcagctttt
     1561 tgtccttctt ctatcatcgt ttgatggaag cgggcagcta cttcttctgg aacagtgaaa
     1621 tctggatact cccaaccata aacttcttta gccatcttga tgccatcttc accaagagga
     1681 gcaccatgaa cagcagatgt tccttcttta ggagcaccat agccgatgac tgtttttact
     1741 tcgatcaatg ttggtttgtc tgtttcagct tttgcttctt caatcgcttt agagattgct
     1801 tctaggtcat ttccgtcttt cactaagata tgttgccagc catatgcttc atagcgagca
     1861 ccaacgtttt ctgtgaaagc ttttgaagtt ggaccgtcta gtgaaatatc gtttgaatcg
     1921 tataaaacga tcaatttccc tagtttcata tgacctgcca tcgaacttgc ttcttgagat
     1981 accccttcca tcaagtctcc gtcaccgcat attgcatacg tatagtgatc catcacatta
     2041 aagttttctt tgttatacgt tgctgcaagg tgcgcttctg ccattgccat accgacagcc
     2101 attgcaatcc cttgtcctaa tggccctgta gtggcttcta caccatctgt atgatgtact
     2161 tcaggatgtc ctggtgtttt agaatcccat tgacggaatt gtttcaaatc atcgattgtc
     2221 acttgatagc cagctaaatg aagcaagcta tacaacattg ctgacccatg tcctgccgaa
     2281 agaacgaaac ggtcgcggtc agcccagttt tttgatgtcg ttggatttac tttcaggtgt
     2341 tttgtccata acgcatatgc cataggtgct gcgcccatcg gcaatccagg atgtcctgag
     2401 tttgcttttt ggactgcttc aatacttaat gtacgaattg tgttaacgcc aagttggtca
     2461 attttgtcaa acaaaataac ccctcctatt aatttaggtt ttttttacct ttatttctta
     2521 cactttcatt ctataagatg tcacacgaaa aagcaatctt tttcagtatt ttttactaaa
     2581 agttgtcatt tcggttgtgt aagccttttt tcttttggat ttcttttaat ttttccgggg
     2641 tcacgtcatt gccctcagga tcgacgactt tcattccttc gatatgatga cgcataccgg
     2701 aacgaaaagc ttccaaatac tctttgcgca agttgatttg ttcttgttct tcggcttttg
     2761 agagaccatt ttccttcttt ttacgtgcaa gttcattgat ccgctctatt ttttcaggtg
     2821 acagcatgct cagccctcct tttactctcc ttcatattat ataaaaactg aaaaaaaaac
     2881 aactagataa actaaataaa atacgaacat tcgtttgatt tagcgctttt tatatggtac
     2941 tatttattta tcaagtgaaa gaaggtgtac cttgtggccc gacaaactga atcaagacaa
     3001 atcgaagtct tacgatatat ccatgaacaa gtaacccaaa aaggataccc gccgactgtc
     3061 cgtgaaatcg gggaagctgt tcagctttct tctacttcta ctgttcacgg tcatcttgca
     3121 cggcttgaaa aaaaagggtt tatccaaaga gatccgacga aacctcgtgc aatcgagttg
     3181 acaaaacaag gtttagaaaa gattgggatt cgtccaacaa ctattccagt acttggcgtc
     3241 gtaacagctg gagaacccat tttagcagta gaggatgctt cagacttctt tccaatccct
     3301 cctaatcttt cttccgaaga aggcagtctc tttatgctga caattcgtgg agatagtatg
     3361 atcaatgctg ggatattgga tggtgacaac gtgatcgttc ggaagcaaga aactgctcaa
     3421 aatggcgata ttgtcattgc aatgactgcc gaggacgaag cgacttgcaa acgattctat
     3481 aaagaaaaaa actttttccg tctgcagcct gaaaacgact tgctcgaacc aatcatctta
     3541 gatcaagtat ctatattagg acgagtagta ggactttatc gtagtcatat ctattaattg
     3601 ctactaatta aaagaaaact ttttaaaaag agtttgagac gttggtctca aactcttttt
     3661 tcgcccgggt tcttcttctt tcaaattttt atcacttccc tctttacaaa ccgaacaaat
     3721 attcgtatac taaatatacg aacaattgtt cgcataaatt aaagaggtga gaattcatga
     3781 aatcaatgag acgtcttggc ttagtattaa ctgttttgtt tttaggtatt attattggta
     3841 atttcggcat acgtccagct ttacttgtgt ttgttccgtt atttactttg tggttcatgt
     3901 tgtgggatga aaagaagtac cgtcaatctg agcggcgcag aattcatgaa gatcatttct
     3961 atcatgagcc ctactacaga tccaggcaat cataataagg attgactgct aattattcaa
     4021 aagacaag
//
LOCUS       DANJJH010000149         3963 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000149 DANJJH010000000
VERSION     DANJJH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(588..1544)
                     /locus_tag="PWS53_002719"
     CDS             complement(588..1544)
                     /locus_tag="PWS53_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872899.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(1653..1898)
                     /locus_tag="PWS53_002720"
                     /pseudo
     CDS             complement(1653..1898)
                     /locus_tag="PWS53_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2043..2465)
                     /locus_tag="PWS53_002721"
     CDS             complement(2043..2465)
                     /locus_tag="PWS53_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872900.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2645..3016)
                     /locus_tag="PWS53_002722"
     CDS             complement(2645..3016)
                     /locus_tag="PWS53_002722"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0872901.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(3350..3613)
                     /locus_tag="PWS53_002723"
     CDS             complement(3350..3613)
                     /locus_tag="PWS53_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872902.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1202 a          825 c          621 g         1315 t
ORIGIN      
        1 gagattgaat gctgatttac ctatgtttta cgtaatggtc tttagttatt tttgctcata
       61 aaatgcataa aacacgcgtt tttatgtgtg cttgaaattt cttggctcta taatttctaa
      121 gactgataaa aatattctat cggttctttt tgcaaataaa aaaacatata gaagctccat
      181 tagattgaat caccacaaca caatcaaagg agcattccgt atgcttgatt ttcattctat
      241 aaaactactt ttaggaatca aagaaaatca caacggttca aactaaaagc aagaatatcc
      301 gaagtaccat tgtttcatcg atcatgagaa aagcattcac ctctgtctgc agatgttctt
      361 atatcgctct gtcagtccta ttaataaagt actgttttgt tttcaaaaaa actattacga
      421 gtacttttta agagaatgcc ttgcaaatat tcatcacaca ccgttatata agtcaaatag
      481 tttctttcag tcgtcaagaa aagattctcc tgtccttggc attatcagtt aagtagcaac
      541 ttaattctcg gacttgttct actctagcaa aatttatttt catcaactta cgtccaaatg
      601 tttttttgaa taattacttc atcgcgggtc tggaagactt gcctcattcg ataagtcact
      661 agcttttggc gtccttcagg atttctagta tacaccaact gacatttggg cagcgacgat
      721 tgatttaatt cgtttagaaa aaggttagcg atttgcttat ttcggccaaa gcgttctggt
      781 accgtcagaa ctatcttttt cccttcgatt aaataccgcg gattctccag cggtgctaaa
      841 agctccctca tagcttcagt aaaaagctgt ccttctcgat agccagcatt ctcaagatag
      901 catctaaatt cctgctgatc agccgctacg attacttttc tatcactaac ttcaccaagg
      961 cgctgcaacg tcgtttgaac gcatatggcc aagctttttt tgcgggcaca ttccttttcc
     1021 catagctttt gtcgcctacg aatttggaaa tattccagca agttaccttt aaatatagct
     1081 gaccagaagc ttagaggctc tatatttttt actactatcg gcaatgaagt ttcttgtttt
     1141 tgctttacct gatcgacttt cctaacgacg attggcatag ataggtgcca gccatcagct
     1201 aaagcagtac tccattgttc tttcaaccga cttcgttcac aagaaaaata gaacatcgac
     1261 tgagtataag tttccatcgt ggtggtcgta tattccagtg gtaaagcaaa gcgatcgcca
     1321 ccactctcaa tttttggtga agattcaaag gtcagacctg aaatattgcc caaacgccgt
     1381 agcattcttt tcaaatcagg ctgttcagac agaggaactc ccggtaatcc aatcgctaaa
     1441 tgccaaatgt tcgttacttt attttgtgca cctaggcgta agccccgccc tcgtagttgt
     1501 tgtgtttgca caaaagtgga agcttgatta gccaagatca acagattaat tgcaggacaa
     1561 tcccagcctt ctcctagcaa actaacggtt cctaccaaaa tattcaactt cttttcggtt
     1621 aataggcgag taactgcttg catcaatgct tggcggtttt tgtcatccac cgtcaaataa
     1681 aaataacccg acatataagg atgttcattc atacccgtcg ctagttctgc cagctcaaca
     1741 gttactagca ttttcggtaa cagtaagaac tctccacaga tggcaccgat ttgctgagtg
     1801 ttacctaata gacgaacaac ttcatcaaac aaaggaacca cgccataagc taaggtacca
     1861 tcactgcctg ctaatacctc cttgcaaata cgatccatca aaatgaccgc tgtcagttct
     1921 tgcttcagct gaacttcttc ttttaacaaa atttgacaaa ctgcttgctt tttctcagga
     1981 atcgcctgac taccaacagt gtctggatac tctggaaaca aatgcagcga ctcatcaaac
     2041 aatcatccag cttttttcaa ttgataaaaa ataggcaatt ccggttgatc ttcaaacatc
     2101 tgctgataaa tagtgatcag ctctttttta cctaaagatg gaactgaagc ttttttacct
     2161 tgctgcttag aactgcctaa tagctgccaa tgttcgggta tcaattcata gttatgatga
     2221 ttcaagtaaa ttaatgcact agtgaacagc tcaaaatttt cataaatttc cttcgaatgc
     2281 tcaagcggat gctgaacaaa ttctaaagaa agcaaataat ccaccgtctc ttggctttcc
     2341 attaacgctt ccaccagctc attttgtttc tctaaaaagg taaaataatg ctgagtagtt
     2401 tcctgattag ccggaatcaa atatagaaaa tcctgatgag gacatagaac ctcctccttt
     2461 accaactcgg gaatgctaat ttcttcgtca atcaggccat tttaactcaa tatattgctt
     2521 ccactccgtc tgagagctat caatcggagg cgtggctgtc aaggaaatcg tcaacagatt
     2581 cggttgtttt ttcttcaagt caatcaacaa ttcaccccag ctttttttta aatgatgagc
     2641 ttcatcaaaa aactaacagc tccagctgat caccataagt tggctcctcc ttcagtcggt
     2701 tgtaaatggt ctgatacgtt tccactgtaa tatgactagg atgttcaatc gccccagaaa
     2761 gatagcaact atctgctgga tttactaaga aatattcctc gattgttgtt aaccactgtt
     2821 tcttcaatat tagggaagga accaaaatta aggttttaca atccaaacgt cggatcatct
     2881 caattcctaa aaaggtcttg ccagcccctg gtggagccac tagatggact gaattgttat
     2941 ttaaataagt agcaaagtta gtcaatatac gcttctgata gctacgccac tgaatttttt
     3001 catcagatag ccacatccca ggtccccctc tcacgtattt tctctatcat aatctaaatg
     3061 aatacagcaa aaaaaagatt tcttcaagaa tatgtgtttt tatagtaata ttttacttac
     3121 tctaaactca aatgaaaagg taaaaaaact ggtagtacaa tcttttaaat tctcattgtt
     3181 ttgtacgttc tttactatca aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt
     3241 tttagctcta taatttctaa gactgataga aaatcattct gtttttgaag tcttttttca
     3301 taaatactac ttgatttatt gtgtatattg tataaaagat tgttcataat tattttttat
     3361 aattaaaatt tggtattgat gaccagcgat tccgtatata atgcgcaatc aaattaagct
     3421 ttctgtctct ggtaaatatt cctgttctgt tttcttgtac tcgtaagatt tcttcactta
     3481 tcgcaaaatt cataaaattc cacacttttt cataaacaaa atgtacacat ttatctatta
     3541 cttcataata gtctacttga tattcttctg taaacatggt tgctactgta tctgcaccat
     3601 atttgataaa catttgggct actcccttaa attgagaaaa gcccattagt ttatctacta
     3661 atacttctaa aatttcttgc tttcttcttt aacatcgtcc cgtaaggaat catgtagtat
     3721 gctggaggaa gttcattttc taaataattg tatcgcttca attgctcaaa ggctattctc
     3781 tacaattaat tacaccaatt actttatctt cacttatata agatttctaa acatttttcg
     3841 tcaaatgaag agaaaatatt tttttaaatg atcttgctca agatttgtca acgaatcttt
     3901 gaattcgttt tgaagttctg aggaaggtat catttaaaaa ctcaatctga ctttcatcat
     3961 ttc
//
LOCUS       DANJJH010000150         3941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000150 DANJJH010000000
VERSION     DANJJH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1466
                     /locus_tag="PWS53_002724"
     CDS             <1..1466
                     /locus_tag="PWS53_002724"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL0872903.1"
                     /translation="QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDIL
                     GYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTD
                     EQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSD
                     MMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAK
                     MNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRF
                     NRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSI
                     YAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKI
                     LETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQ
                     EQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1456..2673
                     /locus_tag="PWS53_002725"
     CDS             1456..2673
                     /locus_tag="PWS53_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL0872904.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2686..>3941
                     /locus_tag="PWS53_002726"
     CDS             2686..>3941
                     /locus_tag="PWS53_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0872905.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1364 a          674 c          821 g         1082 t
ORIGIN      
        1 aacaagtggt tcttttagca gatgaatcct tagaggaata tgacacagta agcaaacaaa
       61 caatgttgta tcgtgaatta ctttcagatg aagaatcaaa agaagatctg attgccacaa
      121 tcgttgatat tttgggatat gcaattgcac cagagtatct tttcaacgtc ttagcggatc
      181 aagcaaaaca agcaacgttc caattgaacg atctaaacaa agcctttgtg caattggcat
      241 ctacctacaa tcaatttaat gggttatttg atgatgtgga tctccaatca aaaaaactag
      301 gaacagatga gcaacaacga aatgtgacga ttaccgaagt catcaagaaa ttgaatgatg
      361 tagatgtttt agaacacgat ggggacgtga tcggggatgc gtatgagttt ctgatcagtc
      421 aatttgcttc agaagcaggg aagaaagccg gagaatttta cacacctcat atggtttctg
      481 atatgatggc gcaaatcgta acgctcgatc aaaaggaacg acgattcttt agtgtttttg
      541 atccaacgat gggttcaggt tcattgatgt taaacgtacg taactactta acacacccag
      601 ataacgttaa atatcatggg caagaactaa atacaacgac atacaatcta gcaaaaatga
      661 acttgatttt acatggtgtc gacgccgaag aaatgaatct ccgcaatggg gatacgttga
      721 ataaggactg gccaacagat gaaccttaca catttgatgc agtagtaatg aacccacctt
      781 attcggctaa ttggtcagca gatacgactt tcttagatga ttctcggttt aatcgttatg
      841 ggaaattagc gccaaaatcc aaagcagatt ttgcgtttct tcttcatggg ttctatcatt
      901 tgaaggaaac aggtaccatg gccatcgtct taccacacgg agttttattc cgtggcgctg
      961 cagaaggggt catccgtcaa aaattactag aagatggcag tatttatgct gtgatcggta
     1021 tgcctgcgaa cttgttcttt gggacatcga ttccaacgac ggtgattgtg ttgaagaaga
     1081 atcgtcaaac tcgagatgta ttattcattg atgctagtcg tgaatttgtt aaagggaaaa
     1141 atcaaaataa actatcggaa gaaaacattc aaaaaatcct cgaaacctac gctgaaagaa
     1201 aagatgtcga gaaatacgct catttggcaa cctttgatga aatcaaagaa aatgactata
     1261 acttaaatat cccacgctat gtagatactt tcgaagaaga agaaccaatt gatatggttc
     1321 atgtcggaaa tgacatcaag aagatacgac aagaacagca agtgctagaa aaaaaacttt
     1381 tagaagctat ctcttccctg caaacaacac ctgaaaatga agcgtggtta caaggtgcgc
     1441 tggaggtgtt caaacatgag caatgataca caaccagaaa tccgtttccc aggatttaca
     1501 gatgattggg aacagaggaa gttgggggac ttcgttgtag attatgttga aaagacaagt
     1561 gtacaaaatc aatttcctat gctaacttca tctcaacaaa aagggatagt tttgcaagaa
     1621 gactattttg caaatcggca agtaactaca gaaaataata ttggttactt tgtactacct
     1681 agaggatatt tcacgttccg tagtcgaagt gataatgatg tgtttgtgtt taaccgtaat
     1741 gacattattg atagagggat tataagctat ttttatcctg tatttacttt aaaatctgct
     1801 gattccgact tcttcttaag acgtattaac aatggaatcc aacgacaatt atctattcaa
     1861 gcagagggaa caggtcaaca tgttctgtct ctaaaaaagt ttaaaaatat agtagcaatg
     1921 tttcctagtg aggaagaaca acaaaaaatc ggcaccttct tcaaacaact tgacgacacg
     1981 atcgctcttc atcagcgtaa gttagatttg ctgaaggaaa cgaaaaaagg attcttacaa
     2041 aaaatgttcc ctgaaaacgg agcaaaagtg ccagaaatcc ggtttcctgg ttttacggaa
     2101 gattgggaag agcgtaagtt gggagaatta ggtaaaactc aatcgggaat tggatttcct
     2161 gatgccgagc aaggtggttc tgaaggtact ccatttttta aagtttccga catgaataac
     2221 attggtaatg agtatgaaat gagaaatgca aatcattatg tatctaatga gcaaattgag
     2281 cgtaaaaaat ggaagccaat aaaagatgtg ccagcgatta tatttgctaa agttggcgca
     2341 gcaataatgc ttaatagaaa acgtttggtc acaagccctt tcttaataga taacaatacc
     2401 atggcttatt tatttaataa tacttgggat atctattttg gtaaaatatt gtttgaaact
     2461 ataaatttgc caagatactc ccaagtaggg gcattgcctt cttataacag ttctgatatt
     2521 gagaatatca gtgttaaggt tcctgtaaag gatgaacaac aaaaaatcgg tactttcttc
     2581 aaacagctag atgatacgat cgctcttcat caacgtaagt tagatttact aaaagaaacg
     2641 aaaaaaagct tcttacaaaa gatgtttgtc taggagggaa gaagaatgaa aattagtcac
     2701 cgtgaggaag cagaagtcga agaacagttg attcgtgtac ttggagaagg gcataatcag
     2761 tggacgtatc gtccggatct aaaatcagaa gaagaccttt gggtcaattt acgccaaaaa
     2821 attatctcaa ataatcaagc agaactgaat gattttcctt tgaccgataa agaatttgaa
     2881 acgatcaaaa cggaattact gctgcgaacg aaaaccccat ttgatgctgc gaaatggtta
     2941 aaaggtgaaa atgggatggc tcggatcacg attgaacggg aagattcgca attgggttct
     3001 gtctcactta tcctttattc caatcaagac attggtggag gaatctctac ttatgaagtg
     3061 gtccatcaaa ttgcaaaacg tggaagtaac atagaagcac gggatcgacg ttttgacgtc
     3121 accttattaa tcaatggatt accgattgtc caaatcgagc tgaaacaagt aactgcaaaa
     3181 gatggtgttt atcaggcatt taaccaaatc aaaaaatatg cagaagaagg tatgtttcgg
     3241 aacaatatct tttcgacctt acagttgttt gtggtttcca atgaacaaac cactcggtat
     3301 tttgcaaatg ccttaccgaa agacttgcac cctaagtttt tatttagttg gcgaacgaaa
     3361 gacaatgaaa aagtagaaaa cttatatgag ttttgtaaac aagtgttaaa tatcccggat
     3421 gctcatcgtt tgattgcgga ttatacaatc gtcagtgaag atcaagacaa taaaaccctc
     3481 atggtgttac atccttatca agtacatgcg attcaagcct tgtttattgc cgcaaataaa
     3541 catcaatcag ggtatgtgtg gcatgcgact ggttcaggaa agacgttgac tagttttgta
     3601 tctactaagc ttttagcacg aaaatcgggg atcgatcgaa cgatcatgtt agtggaccgc
     3661 aaagatttag ataatcaaac caccacagag tttacaaaat ttgcttcgga atttaatact
     3721 gggatttctt ccgggaacgc caaagcgaat agcttgatcg ttggaacggg aagtgccaaa
     3781 gaattaagtg aaaccctgtt ggcagatgcc aatgcaaatg ttgtaatcat tacaacccgt
     3841 caaaaactag atgcggcgtt gaaatatgca aagaaacaag aagagaaaaa aggaaccaat
     3901 cgcttccaaa aattaatggg ccaacatatc gtgtttgtag t
//
LOCUS       DANJJH010000151         3737 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000151 DANJJH010000000
VERSION     DANJJH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWS53_002727"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWS53_002727"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL0872906.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWS53_002728"
     CDS             complement(1172..1867)
                     /locus_tag="PWS53_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL0872907.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWS53_002729"
     CDS             complement(1880..2596)
                     /locus_tag="PWS53_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL0872908.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWS53_002730"
     CDS             2926..3534
                     /locus_tag="PWS53_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL0872909.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
BASE COUNT         1072 a          766 c          664 g         1235 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaat
//
LOCUS       DANJJH010000152         3711 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000152 DANJJH010000000
VERSION     DANJJH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..333)
                     /gene="rpmA"
                     /locus_tag="PWS53_002731"
     CDS             complement(40..333)
                     /gene="rpmA"
                     /locus_tag="PWS53_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL0872910.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(346..690)
                     /locus_tag="PWS53_002732"
     CDS             complement(346..690)
                     /locus_tag="PWS53_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL0872911.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(717..1025)
                     /gene="rplU"
                     /locus_tag="PWS53_002733"
     CDS             complement(717..1025)
                     /gene="rplU"
                     /locus_tag="PWS53_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL0872912.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(1045..1121)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1307..1948
                     /locus_tag="PWS53_002734"
     CDS             1307..1948
                     /locus_tag="PWS53_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0872913.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(2010..3641)
                     /locus_tag="PWS53_002735"
     CDS             complement(2010..3641)
                     /locus_tag="PWS53_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL0872914.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1136 a          824 c          656 g         1095 t
ORIGIN      
        1 caaaagtctt gtcacaacct ctttaatcat aagattcaat taagcttctt gagctactgg
       61 ataaacagac acttgttttt tgttacggcc tttacgttcg aaacgtacta cgccatcaac
      121 ttttgcaaat aaagtgtcgt ctccaccgat acccacgttt acacctggat agatttttgt
      181 accgcgttga cggtaaagaa ttgatccacc tgtaactgtt tgtccatcag cacgtttagc
      241 acctagacgt tttgattctg agtcacggcc gttagatgta gaaccgccac cttttttgtg
      301 ggcaaataat tgtagattca tagataacaa catggtctgc acctcctatt tatcattaat
      361 tgttttaatt tggatatatt ctgagttttc agcttctatc gattgtaatc ctaataaaag
      421 attttctaat agaatctcag caatattgtt ttgttcttgt gtcattcctg aagtgacttc
      481 ggtataaaga taaccgcctt cttcttcatt catttcaaca atcggctcaa aaccggctaa
      541 tgaggcaatc ccgttgacag tgctgatcgc taacgcagaa acacccgcac aaacgatatc
      601 acttccataa ggaccagcat cagcatgccc ggtcaaagtg aatgaaacga tctgacctgc
      661 gtcatttcgc ttaaatgttc ctttgatcat atataggcct tctttctcaa acgctattaa
      721 gcgttgatcg cgtcgataac cacttttgta tatggttgac gatgaccttg tttacggtgt
      781 gaatgttttt taggtttgta tttgaaagta acgactttct tttgtttgcc gtgtttttca
      841 acagtacctt caacagttgc acctgcaaca gttggagcgc ctacttttgt agattctccg
      901 cctactaaga taacttcgtc aaaaacaact ttttctcctg cttcaacgtc aagtttttca
      961 acatagattg cttgtcccac ttcaactttt acttgtttac caccagtttt aataattgcg
     1021 tacatgctat aaagcacctc cttctgatat acttagactc gccatccgaa gtgacagcat
     1081 atgctgtgct tattacttgt tctgagcggt tgtagctgtg gagtccacaa ttacaacagt
     1141 actagcttac cagaacagca accgttagtc aactaaattt aaaatcaaaa agtaaaacaa
     1201 aaattcaagc aatacttctg cttttctatc ctcacagttc taccaaataa aatagtcgca
     1261 ttgtgtttct tttattctta taataaaaga aaaaggagga gcataaatgg aatacccaaa
     1321 agaactgata caagaagctt ctttaagaat gaacggccgt cctgtagaag gatttatcgc
     1381 tgggcaagga cctcttcatc ctaaactgat gctcgtggga gaagccccag ggaaaacaga
     1441 gattgatacg catgttcctt ttagcggtca agctggaaaa gaattgatgc aggcactttc
     1501 ttcaactggc ttaacacgag aagaggtgta tatcaccagt gctgtaagga gccgcccgta
     1561 tcgcgtgact catcggatca ataaacggac acaacagtca gaaactgttt atccaaatcg
     1621 gacacctact cgttccgaag tatttgcgca tgcaccaata ttggattacg aactgatgaa
     1681 ggtccagcca aaattaattg cgacacttgg aaacattggt cttcaacgtt tacttggcaa
     1741 agaatatacg gttaccaaaa atcatggtca actctataca gggcctgtct tacaattaac
     1801 agaacaaaag gatgcctacg aatggtcaga aaaaaactat cgaattcttc cactttttca
     1861 tccagcagct attttctaca atcgctccct tgtacctgtt atccatcaag actggcaagt
     1921 actagcttca cttttacagc aatattaaaa agaacagaca agaccataaa atcttcagtt
     1981 tacagtcttg tctgttcttt ttcattgttt taatggctaa ttgcagtata tttcgcatgt
     2041 aaagctgtca acgcatgagc aactgtttga tgttgatgcg tgaatgtatg ttcccacaag
     2101 tgagaaagtt gtttttctac ttctggctgc aaggaagtat agataatcgt ttgtcctctt
     2161 tgctctgctt tttcttgaaa ttcatcaaaa actgcacgtg ccgtcaaatc aatacttggt
     2221 acgcctcgca tggagaaaat gacaccatcg tgatgattta gttcttccaa tttatttttg
     2281 agtttttctg aagacataaa aaacagtgga ccactaatat acactactgt ccagttattc
     2341 agcttttccg tttctggcag ccccattctt tgccaatcga tcgtttctaa tgaaatcgtg
     2401 atagcagcgc ttttgacaac aaaaaataca cagccgccga tcaccccgat cacgatagca
     2461 atacttaaat caaagataac tgtacaaatc atcgtaagga taaacaatag aactgctgac
     2521 caatatttat tagtaaatag ctccttgatc gtttcccatt cattcatacg ccaagccgtc
     2581 acaatcaaaa cacctgcaag tgctggcatt ggaatctgtg acatcactgg cgcaaaaaca
     2641 agcatggaga gcagtaagac aactgcatgt atcataccag ctaatctagt ttgtgcacca
     2701 gatttgatgg ccacgcttgt acgggcgatg gccgcagtag caggaatccc gccaaagaaa
     2761 ggaagcaaca gatttccaat tccttgcgcc attaattctt gattgctatc taagggctta
     2821 tttgccattc ggccagccga agcaccacat agcaagcttt caatcattcc taataaagca
     2881 atgctaatag ctggaactgc cacttgttgc aaggcagaaa agctaaaatc tcccatattc
     2941 aaacgattac tactgatcaa tgtttgtgga atcttaccca cagttgctac tggtaaatgg
     3001 aacaatagca taactgctgt tgccaaaatg atagccagca gcgaactagg cattcgtttc
     3061 gcccattttt ttggatagat aaacatccct aaaatgacta gccctcccat gatcaaagag
     3121 gctgtgttta tcggaaatcc tatcgtttga taactactta gtttttcaat aacgtttgta
     3181 ccttccgagt gaacaccaaa taaattatcg atctgaccta atgcaataat gatagcaata
     3241 cctgaagtaa accccgtaat tacgggtgct gggataaaag aagtcagtgt tcctaatcgg
     3301 aaaagtcctg ctgcaagtaa aaatagaccc gctaaaaaag tagcaagcaa tacaccttgc
     3361 attccctttg ttgccgcaat agacatcaag atagctgcca tcgcacctgt cggacctgat
     3421 atttgataaa aaccgcctga taaaccgcca ataacgatac cggcaatgat agccgtaatc
     3481 aaacctgcgg ccgcatcagc tccgcttgat accccaaaag ccaaggctaa aggcaatgct
     3541 acagcagcta ctgtgatccc agccaaaaga tctttctgaa atcttgaaag atcataacct
     3601 gaaaattctt tttttagtga ggaaacataa tttttgaaca cctatctaac tcctttttca
     3661 tgaaattttc taaaatacca ttcattagaa tatcatattt catgagataa a
//
LOCUS       DANJJH010000153         3667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000153 DANJJH010000000
VERSION     DANJJH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            158..2257
                     /locus_tag="PWS53_002736"
     CDS             158..2257
                     /locus_tag="PWS53_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0872915.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3108
                     /locus_tag="PWS53_002737"
                     /pseudo
     CDS             2453..3108
                     /locus_tag="PWS53_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3213..>3667
                     /locus_tag="PWS53_002738"
     CDS             3213..>3667
                     /locus_tag="PWS53_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0872916.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1480 a          607 c          737 g          843 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat attagaacgt
     2941 tctgcagatc agcaaatcaa ctttacagat aaatcggatc cagaagaaat catgaaagct
     3001 tttggtatca gcaaaggagc atttaagcgc gcattaggta atcttttgaa acaaggattg
     3061 attacacaag aaaacggtgt gacaaaacta gtcaaaaaat ctaattgaga atcccttgca
     3121 tgtttttctt aactagctta taataatttt aattaacata gtgttatatc tttttttatt
     3181 tgtaattatt ataaattgtt ggggttgagt aaatggtcat gaatgctatt tcggcgatga
     3241 aaaaaacaaa acagcagcta catgaagcgg ggtttaagct gacaccgcaa agagaagcta
     3301 ctctgcttgt gcttttggaa aatgagaaag agcatttatc tgcagaagaa atcttctttt
     3361 tagtgaaaaa aaagagtccg gaaattggac ttgctactgt ttatcgaacc cttgaaatct
     3421 tgacagaatt gaaaattgtc gataaagtaa gttttaatga tggattagcc cgttatgatt
     3481 tgagaaaaga aggcgccaaa catttccatc atcatttact atgcttgaac tgcggcacga
     3541 tcgaggaagt agaagaggat cttttacttg aagtggagca agtagtagaa gaacgttatc
     3601 attttttagt aaaagatcat cggctaacgt tccatggaat ctgtcaggaa tgctatgaaa
     3661 aagaaaa
//
LOCUS       DANJJH010000154         3578 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000154 DANJJH010000000
VERSION     DANJJH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            159..1925
                     /locus_tag="PWS53_002739"
     CDS             159..1925
                     /locus_tag="PWS53_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL0872917.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1940..2377
                     /locus_tag="PWS53_002740"
     CDS             1940..2377
                     /locus_tag="PWS53_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0872918.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2390..3541
                     /locus_tag="PWS53_002741"
     CDS             2390..3541
                     /locus_tag="PWS53_002741"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL0872919.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1197 a          636 c          772 g          973 t
ORIGIN      
        1 cagttttatt taaaattgaa gttttctgat accgaaatct ttttattttg ctgacatact
       61 caaacctttg gcacaaagtt tcgtgccaaa gtacgactat ttctcttttt agtaagcgga
      121 tacaattaga gtatcaaaag gagagaggga gagacaaaat ggaacggacg attcccaaaa
      181 acaacatttc gctaaaagcg agggtacaaa aattaggcag tactttatca agcatggtca
      241 tgccaaatat tggagcactg attgcctggg gtgtattaac ggctctattt atcccagatg
      301 gttatttacc aaatgaggct ttggctacca tggtaagccc aatgctaact tatctgattc
      361 ctttattgat cggttatact ggcggaaaag taattgctgg tgaccgcgga tcagttgttg
      421 gggcaattgc aacgatgggt gtgattgtcg gaacagatat tccaatgatg ctaggcgcga
      481 tgatcatggg acctcttggt ggatatgcca tcaagaaatt tgatcagcta ttccaaaaac
      541 ggatcaaatc cggttttgaa atgctagtca ataatttttc agcaggtctt atcggttttg
      601 gattagcgtt attaggtttt tctgcgattg gtccagttgt cgatgcattg actcaagcaa
      661 tggcaaaagg tgtagagatc attttatctg cccatttgat tccattgaca agtattttta
      721 ttgaaccagc aaaaatctta tttttgaata atgcaatcaa tcatgggatt ttgacaccat
      781 tgggaactga acaagtagca gaggctggaa aatctattct attcttgctt gaagcaaatc
      841 ctggtccagg acttggcgta ttacttgctt ttactttgtt tggcaaagga gcagcaaaat
      901 cttcagcacc aggagccatg atcattcact ttttaggcgg gatccatgaa atctattttc
      961 cttacgttat gatgaaaccg ttattattct tatcagttat aggcggagga atgtcaggaa
     1021 gtttcatttt ccaattgatg gatgctggac taagagcgcc agcttctcca gggtcgatca
     1081 ttgcgattct agcgatgaca ccgttcaatt cttaccttcc tgttattttg ggcgtgattg
     1141 ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt gaaagcggat gcgaaagata
     1201 caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc agctaagcaa gcttccaaag
     1261 gtgcccaagg tattcctcaa ggagctacat tctctggcgt acaaaaaatc atttttgctt
     1321 gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat cttgagaaaa aaagtacagg
     1381 aagcagactt gcctcaaacg gtgaccaatc aagcaatcag ccaactgaca gatgactcga
     1441 atacattgat cgttactcag gtcgaactac aagaaagagc aaaacaaaaa gcaccgagcg
     1501 cgcgttttgt ctcagtagaa aactttttga attcacctcg atatgatgaa atcattcaag
     1561 aattaagcag caagcagaaa acatcagttt ctgaaattga gaatgagcca gcagaagagt
     1621 ttctttcagc aaacatcaaa caaatcaaag agattctact tgtccacgat gatcgttccg
     1681 gatcagcaac aatggggatg accgtgctca aagatatatt agacaagaac catttaacta
     1741 tcccgttaag aaaagtcaat atccaagatt taaaagatga acctgattca ctgatcgtag
     1801 ccaaagaaag tttagcggaa aaagtacaga tggctgcacc tcaatccatt catttatcag
     1861 tcaacagcat ggtcaatccg caaaaatacg aatcgattgc acaatatttg aaacagacag
     1921 cttaagggag gaaaacagca tgcaaagttt gccattcgac atgatcgagc tcaatcgatc
     1981 ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag atgcttgtag aagcagggtg
     2041 cgttgatgag gaatatatcg atgccatggt agatagagac aaaatgttat ctgtttatat
     2101 ggggaatttt atcgcgattc ctcatggaac agatgaagca aaaaaatatg tgaaaaaatc
     2161 cggcatctgt gtcgttcaag taccagatgg tgtagatttt ggtaccgaag aagaagaaaa
     2221 gattgcgact gttttgttcg gtattgcagg agtcggagat gagcatttgc aacttgtaca
     2281 gcaaatcgca ctttattgca gtgatatgga caatgttgtc cagctagcag acgcgctcag
     2341 caaagaagaa gtaacggaaa atctagcaat tgcttaagga gagagaaaaa tgagagcagt
     2401 acattttggt gcaggaaata tcggtcgagg atttattgga gaggttttgg cagctaatgg
     2461 atttgagatt actttcgtag atgtgaatca cacgatcatt gatgctttaa atgaaagaaa
     2521 agaatacgaa atcgaattag cagatgaatc aaaagaaaaa atcactgtaa aaaatgtcaa
     2581 aggaatcaac aatcaaacaa atccagaaaa agtaatccaa gaaatcgctg aagcagattt
     2641 agttactacc gccattggcc cgaatatctt accttttatt gctgagttga ttgcaaaagg
     2701 tatccaagaa agagaaaagg aaggaagcac aacatcttta gatatcattg cctgtgaaaa
     2761 tatgattggc ggttcagcat ttctagaaaa agaagtgtat aaatatcttc cggaaacatc
     2821 atttactgac aaatatatcg gctttccaaa tgcagcagtc gatcgaatcg tcccattaca
     2881 acaccatgaa gatcctttat tcgtccaagt ggaacctttc aaagaatggg tcattgacga
     2941 tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt ttatatgtag atgatctaga
     3001 accatatatc gaacgcaaac tatttagtgt caatactggt catgcgactg tcgcttatac
     3061 aggtgccttg ttaggttacc agaccattga tgaagcaatg caagatgcgt tagttgtggc
     3121 acaattaaaa tcagttttgc aggaaacagg aagtttgctg attgccaaat gggggtttga
     3181 tgcagagcag catcatgcgt atattgaaaa aatcattcat cgcttccaaa acaaaaatat
     3241 ctctgatgcg atcacacgtg tcgctcggac accattacgt aagttaggtt atcaagaaag
     3301 atttacacgt ccagtgagag aactacaaga acataacttg acttgcccac atttgacggc
     3361 aacaatgggg attattttta attactatga tcctgaagat gaacaaagcc gtcaattgca
     3421 tgaaatgaag atccatgaaa acttggaaca attgattcaa gaagtcacag gaatcaatga
     3481 tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt tatgccaagc aagtcgctta
     3541 aaataggata cgaaaaaggg gaatgtgacg aaattttt
//
LOCUS       DANJJH010000155         3540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000155 DANJJH010000000
VERSION     DANJJH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(133..717)
                     /locus_tag="PWS53_002742"
     CDS             complement(133..717)
                     /locus_tag="PWS53_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872920.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="PWS53_002743"
     CDS             complement(928..1131)
                     /locus_tag="PWS53_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872921.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="PWS53_002744"
     CDS             complement(1141..1563)
                     /locus_tag="PWS53_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872922.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="PWS53_002745"
     CDS             1801..2415
                     /locus_tag="PWS53_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0872923.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="PWS53_002746"
     CDS             complement(2580..3404)
                     /locus_tag="PWS53_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872924.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1170 a          619 c          510 g         1241 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
//
LOCUS       DANJJH010000156         3535 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000156 DANJJH010000000
VERSION     DANJJH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..2303)
                     /locus_tag="PWS53_002747"
     CDS             complement(<1..2303)
                     /locus_tag="PWS53_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872925.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="PWS53_002748"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="PWS53_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          986 a          786 c          560 g         1203 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacac ttttataaaa cgacgaaaaa taaatcacta ccagt
//
LOCUS       DANJJH010000157         3430 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000157 DANJJH010000000
VERSION     DANJJH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1008
                     /locus_tag="PWS53_002749"
     CDS             <1..1008
                     /locus_tag="PWS53_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872926.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="PWS53_002750"
     CDS             1025..1174
                     /locus_tag="PWS53_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872927.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="PWS53_002751"
     CDS             1171..1617
                     /locus_tag="PWS53_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872928.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="PWS53_002752"
     CDS             1619..1756
                     /locus_tag="PWS53_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0872929.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="PWS53_002753"
     CDS             1794..2087
                     /locus_tag="PWS53_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872930.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="PWS53_002754"
     CDS             2084..2308
                     /locus_tag="PWS53_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0872931.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="PWS53_002755"
     CDS             2305..3330
                     /locus_tag="PWS53_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0872932.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT         1213 a          587 c          710 g          920 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat
//
LOCUS       DANJJH010000158         3367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000158 DANJJH010000000
VERSION     DANJJH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            118..1158
                     /locus_tag="PWS53_002756"
     CDS             118..1158
                     /locus_tag="PWS53_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0872933.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1982..2314
                     /locus_tag="PWS53_002757"
     CDS             1982..2314
                     /locus_tag="PWS53_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL0872934.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2933..3274
                     /locus_tag="PWS53_002758"
     CDS             2933..3274
                     /locus_tag="PWS53_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL0872935.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1255 a          457 c          640 g         1015 t
ORIGIN      
        1 tagtatttaa ttaaaaaaca gttttaatgc gtttgcattt tttatttatc tttgctattt
       61 tcgaaaagag taggtaattt tacataaata aaacaacgtg aaagtagagg atctacaatg
      121 actgatttta aattttttaa ggcggataga gtttacaacg aattatttta tcaatttcca
      181 aaagtcttta ttgtttctga cgaatacaaa aaaatgaaag attcaactaa gattgcctat
      241 atgcttttaa aagcaagatt agagatcgca atcagcaaac ggcaaatcga tgaagaaggt
      301 aatgtttatt ttacttatac gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa
      361 aaagcgatag caatcaaaaa agagttggaa tcctttggtt tattattaca aaagcagatg
      421 ggatttaaca aacagttagg gaaaaataat cctaatagac tatatctagc agaattaaaa
      481 gtctcagaaa atgatatcta cttactcgaa aaatttgata gagagaatag ggaaaacgtt
      541 gataaatcag agggtatgaa aatcataccc accctcgacg aaaaatcaga cgctgaatcc
      601 cttggggctc aagagggtat gaaaatcata ccgtgccaaa acgttgataa atcagagggt
      661 atgaaaatca taccagaact taataataat atattagaca ctaatagaca caatatagac
      721 actgaaaaag accgcctaca agatcaattg ttgttagaca attttgagac aattatgaca
      781 aacgacagca ttgctacgtt tgtccctgaa cgagtattaa atttgataaa aacattttct
      841 tcaagttaca gtgaagctca aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct
      901 gaaatagaaa gtgatatttc gatagttttt gaagaactcg atagttatta tgtcaatgca
      961 gaacaagaat tatacacgac actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc
     1021 gaaaatatcc agaacctgat ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata
     1081 gctgctaaag tatcaagtga aaaacgtttg aattatgcaa gctccccaag cactattacg
     1141 aaagactggt tagagtgaaa ctttactgac aaagtgattg ttgcttcatg gagtaacatt
     1201 aaaattaaaa attttttatt attctaatta tattacgaat attacaaaat aatagctatt
     1261 ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc cttttacgtc
     1321 ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat gtttgcatac
     1381 tccttttttt tgagttataa taatacgcga agagagataa tttcattatt taaacaagct
     1441 aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta cattttttgt
     1501 ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca cttttggatg
     1561 ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg
     1621 taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc
     1681 agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag tgataccact
     1741 ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat
     1801 aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga
     1861 acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat
     1921 ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt
     1981 tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac
     2041 cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg
     2101 taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa
     2161 acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa
     2221 acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg
     2281 taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt
     2341 tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta
     2401 gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt
     2461 tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg
     2521 gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt tagaagttag
     2581 acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct agactttgac
     2641 gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt
     2701 gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga tcaatcgagc
     2761 atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt
     2821 atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa
     2881 aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa
     2941 ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga
     3001 ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc
     3061 attagacgag gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt
     3121 gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     3181 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga
     3241 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat
     3301 aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt
     3361 aaggaat
//
LOCUS       DANJJH010000159         3344 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000159 DANJJH010000000
VERSION     DANJJH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            161..373
                     /locus_tag="PWS53_002759"
     CDS             161..373
                     /locus_tag="PWS53_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872936.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(651..2450)
                     /locus_tag="PWS53_002760"
     CDS             complement(651..2450)
                     /locus_tag="PWS53_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0872937.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2574..3170)
                     /locus_tag="PWS53_002761"
     CDS             complement(2574..3170)
                     /locus_tag="PWS53_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0872938.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1217 a          601 c          588 g          938 t
ORIGIN      
        1 acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat actcaaaata
       61 aaaaataaaa aaatggataa aaacgttttc aaatacaagg taatttgtta ttataaaata
      121 aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag cagaagtagg
      181 aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct gtattgaacg
      241 tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag actatcaact
      301 tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg cacctgctga
      361 attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa tataaataaa
      421 gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat gggaattatt
      481 cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat gaaggaatca
      541 agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa cgcatatacc tgcggtggca
      601 ggttatgcgt tttttgttgt ataaagactt ttttcattaa cgatttttga ttagccgact
      661 cccaaaacaa aatttagcag tttatctgaa taagtgattt cttcatctgt ttgtttcaca
      721 ctttcattta gtagtcccat ctcatcggtt atgataccat ccacaccata aaacatcatg
      781 cgactcatag catctgaatc gttgattgtc catacatata cttcttttcc gtcttggtgg
      841 gcagaattaa tgaagttacg gttgatcgta ctatattcca ttgtcagaaa atcagcaggc
      901 gtcacagggg gcccaacaat attgaaaggt aagatgtagc ccacataaaa gtttggttct
      961 gcttctttta aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg atggttctct
     1021 aagataactt tgcggtattt atggacaaat cgttcgacaa ggtcatccga atctttttta
     1081 gaggtcttga tctcaataag taatttttgg tttagcttat gtgctttttc caaataagaa
     1141 tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt tgtcaattct
     1201 ttcaatgtaa gatcctgtgg tgttccattt acccctgtta agtgacgaag atttgcatca
     1261 tgcatcacta cgaaatgacc atcttttgtt tcctgaacat ccatctctac ataatcagga
     1321 tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc attttgggta
     1381 ctcacacctc tgtgagagac agtcactggt tttgtctgaa cagctgaagt cagataatcc
     1441 atattgtaaa gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa accagtgttt
     1501 ttcagcgcag agaaacgcaa gttgggggct tgtgggataa accacttagg gatctcaggc
     1561 aagaagcctt cgtcatccat gaaatcaaca atgatataaa aaatccctac ggtagacatc
     1621 acgatattga ataataaaat cccttgtaaa agcgtcatag caaacacagc gctgatcaat
     1681 gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca aaataacaac gatatatcct
     1741 aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa attggcccgt gatggccaaa
     1801 agacgcgatt tggtcaatga ccagctttcc cgaatggctg ctcgaaaagg acgatctcgt
     1861 agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag gaaaatataa
     1921 acgagtaaaa aggaactgac gatgatccaa cgattagcaa aaataaaatc cataatgaaa
     1981 gcggggattt ttatttttga cagtagatca gaattaaaac ttaatccgct gatcggtaga
     2041 atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt cttcagctga
     2101 agtatcgtca agtgtaagag ttgcttcaaa gagattggtt ctttcttttt aataaaaaaa
     2161 acactcatca aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt catcaaaact
     2221 acgattaacg agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat attgtcataa
     2281 gacaaatagt caattgcccc tcggtgtaaa ataaaacggg tcgtactgct caataagggt
     2341 aaacaaataa ataaaataaa gccatgcatc agcaagacat cacggaaata agcttgtgtt
     2401 ccttttaaaa agtcccaagc attacctaag ctacgtttca aataattcat cctatcacct
     2461 gctcttcaca ttgtagcgca aatctaatga aaaaggaaag ggtgcagtgt gttttgcaga
     2521 ccctttccca gatttaacat tgtcaatcaa tgactaattt tacaaaataa atattatcca
     2581 agaagaccac ttagatattg acctccataa atcaaggcca tactatataa gaaaatcgac
     2641 agtggtttag caatcaaaat gattagtgta tatcgtttca ctgacatgtt tgtcaatcca
     2701 gccatcaaac ataacgcatc gtcaggagca actggaagaa aaatagctag agcaaacagc
     2761 ttctcaaaac ggttttgatt atccagccag ccaatatatt tgttatatgt cttatcgcta
     2821 atcatggaaa gtatgaatgg ctttccataa cgacgcccta gcagaaaaat gataatcgaa
     2881 ccaatgcaga ttccaatata gttatagata aatccagcat aagggccaaa tatcaggaca
     2941 ccagctgcag tactgatccc tccaggaatg attggaataa ctacttgcag gatttggatg
     3001 aagacaaaaa taatcggtcc taaaatgatg gaatcaccta ctaatcctct taacgcattc
     3061 aaatctttga aaacacctaa attaataaaa taaatcgtca gcgcgattga taggcctaat
     3121 ccgataatgg atatgaaatt tatcagtttt cgagaggcag caatattcat tattcatcaa
     3181 ctccttagag gttctttgtt aactatactt tactagaaaa gcaactaact gcgccatttt
     3241 ttgatatgac agaaaacaaa caattttgta agttcatcat aagtataaaa gattgatctc
     3301 aaaaaaacat ccaactaaag acttatttaa taacttgttt tttt
//
LOCUS       DANJJH010000160         3249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000160 DANJJH010000000
VERSION     DANJJH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(25..162)
                     /locus_tag="PWS53_002762"
     CDS             complement(25..162)
                     /locus_tag="PWS53_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0872939.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="PWS53_002763"
     CDS             complement(159..1343)
                     /locus_tag="PWS53_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0872940.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="PWS53_002764"
     CDS             complement(1602..1892)
                     /locus_tag="PWS53_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872941.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="PWS53_002765"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="PWS53_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          878 a          771 c          559 g         1041 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattattaa tttgtcctac tattttttat ttaatccaac
     3121 taatttccgt tgtttttcat tggcctgatc ggctacatat ttaatcaccg ttttgaactc
     3181 ttttgcagta gcattttccg ataaatccgc aatgctctta ttgtatttct ttacaatttt
     3241 cgttacttg
//
LOCUS       DANJJH010000161         3246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000161 DANJJH010000000
VERSION     DANJJH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(1..354)
                     /locus_tag="PWS53_002766"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="PWS53_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="PWS53_002767"
     CDS             complement(614..1162)
                     /locus_tag="PWS53_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872942.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="PWS53_002768"
     CDS             complement(1163..2020)
                     /locus_tag="PWS53_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0872943.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="PWS53_002769"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="PWS53_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="PWS53_002770"
     CDS             complement(2582..2911)
                     /locus_tag="PWS53_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0872944.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3133..>3246
                     /locus_tag="PWS53_002771"
     CDS             3133..>3246
                     /locus_tag="PWS53_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0872945.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          963 a          646 c          421 g         1216 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaacg
     3061 gttctgttgc aaagttttcc aaaaaatcta ttttagtgta aaattgagaa aaaagacaga
     3121 gaggacagag taatgaatca ttttaaaggc aaacaattca aaaaagacgt cattattgtc
     3181 gctgttggtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     3241 gatcgt
//
LOCUS       DANJJH010000162         3199 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000162 DANJJH010000000
VERSION     DANJJH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            301..585
                     /locus_tag="PWS53_002772"
     CDS             301..585
                     /locus_tag="PWS53_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872946.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PWS53_002773"
     CDS             588..1004
                     /locus_tag="PWS53_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872947.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PWS53_002774"
     CDS             complement(1141..1860)
                     /locus_tag="PWS53_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0872948.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="PWS53_002775"
     CDS             2513..2608
                     /locus_tag="PWS53_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0872949.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="PWS53_002776"
     CDS             complement(2910..3155)
                     /locus_tag="PWS53_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872950.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          498 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attacaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DANJJH010000163         3185 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000163 DANJJH010000000
VERSION     DANJJH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..76)
                     /locus_tag="PWS53_002777"
     CDS             complement(<1..76)
                     /locus_tag="PWS53_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0872951.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVK"
     gene            431..787
                     /locus_tag="PWS53_002778"
     CDS             431..787
                     /locus_tag="PWS53_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0872952.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            797..1696
                     /locus_tag="PWS53_002779"
     CDS             797..1696
                     /locus_tag="PWS53_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL0872953.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(2093..3172)
                     /locus_tag="PWS53_002780"
     CDS             complement(2093..3172)
                     /locus_tag="PWS53_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0872954.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          872 a          679 c          719 g          915 t
ORIGIN      
        1 tttttacttc aatataagaa ccctcgatat tttgtgtttc ttcgataaaa tagatgtttt
       61 ttatatattc cttcatagtg attcctccaa agaagtcttt tatttgataa gtttactcta
      121 ccgaagagtt gttaataatc cgtgaacaaa aagtaattct ttttccaata gttgttaaag
      181 gttttttatt ttttatagta ttttttgtta tttaagaaag gaataaggtt aaacaaataa
      241 caggttgctt tttgggtctt tttccgttca agaaactgaa cgaagaaaag ggaaagttgt
      301 ctcttttgtg ataagagaaa tcataataaa cgaatgactt actttccaat aaattgagaa
      361 cagtcagaac cagacaaatt agttgcatct gatgaataaa gtgattattc tatgacatga
      421 ggtgatacag atgaaattag aagtgacaaa agaagcacaa gaagtattga agaataaatt
      481 agaagcagac gatcaacttt tattagatat cgaaaacggt gacggtccat ttgcagaaag
      541 ccaagtcagt tgccaattgg atacggcatt tcgattgatc attgtcaaaa aagatacaca
      601 aacagatcta agtctttatt ctgtagtggc tgatacacca gttggaccaa tcaaaatcaa
      661 agacagtgca atactttaca tggatgatcc atccacactt gctttagaac caacttataa
      721 tagtttacaa ttaaaaggac caagcggctt gagaaaaggc aacttgcaag ttgttcgaaa
      781 agactagagg gataaaatgg gaaaattggc gatcatcagc gacctacatg tggacatcaa
      841 taaattcggc gaatcagagc tgatgcagct ttggcaagtt ttgcaaaagc acaagatcac
      901 tcggcttcat ctagccggag atacggctaa taaagtagac cgttgcctag ctgttgtcga
      961 atttttcgtc aataaaggat tatctacgac atttcatttc ggcaatcacg agttggcaga
     1021 tgtgcccaat gaacagatga tggaccattt tccagaccca cattttttaa atgaacggta
     1081 tattgcttta aatgagcaga ccgttctttt agggatgaat ggctggtacg actatcagtt
     1141 ttccgaattg aaagattacg aggcaatcgt acgaatgaag cgtttgtatt ggtatgatcg
     1201 gttcatccag cgtgggaaaa cagatctcga ggtcaatgca gaaatgcttg cagctgcaaa
     1261 aaatttgcta gaccagctgg aagcaaaaaa acttgaggtg attttatcca ctcatttcgt
     1321 tcctaaacgg gcatttatca tgtatcaatc tgctccttat gagcgttgga acaaattaaa
     1381 tgcattttta ggatcagaat cattcggaga attacttgac cgttattcaa atatcaaaca
     1441 agtagttttt ggccatacgc atcgtcgctt tgaagataaa tccatcggtc acacgattta
     1501 cagctgcaga ccttttggtt attattatga atggcagctg acccgagatt ttgtcttgaa
     1561 agaacagttg ctagacagct atgatcctat gaaattacgt gtcctcttgc gtgcccatca
     1621 cccagctttt caggcctatc aaacacaaca tttgcaagag gaatttgaac aagcgatgac
     1681 aatcgttccg tattgagaca gcattagtaa tgtgtaaaag aaaatacctt gtaaaacata
     1741 aggataagta agagcagtag atccatttat cgtggatatg ctgctttttt tgcggaaaat
     1801 aatcagcgaa tgtttatttg atcggatgat ttgtaactcg cagagtacta gatgcaagcg
     1861 tgtattcttt ttttgaacga taatcacaaa aaaaaatata atcgtaaatt tcaagtttaa
     1921 gttagccacc catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg
     1981 agtgggccct tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag
     2041 ctttccagac acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg
     2101 tcaaccgttc ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa
     2161 tctttcgcgg gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt
     2221 tgccgattgg gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg
     2281 tcccgcgctc ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg
     2341 catcgtgcag tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc
     2401 cgaagtacgc accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat
     2461 ccttcccggc cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca
     2521 gcacggcatc cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg
     2581 ttttccgggt accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat
     2641 tcgggcggtt gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca
     2701 tattcttcgt tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga
     2761 taatggccgg gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc
     2821 atttttccaa cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata
     2881 agggccgtct gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt
     2941 cgtagacgtc atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag
     3001 taacggtccc gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg
     3061 caacctgacg gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt
     3121 aggttaggtg ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc
     3181 atcct
//
LOCUS       DANJJH010000164         3159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000164 DANJJH010000000
VERSION     DANJJH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..443)
                     /locus_tag="PWS53_002781"
     CDS             complement(<1..443)
                     /locus_tag="PWS53_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0872955.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="PWS53_002782"
     CDS             513..863
                     /locus_tag="PWS53_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872956.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="PWS53_002783"
     CDS             1277..2095
                     /locus_tag="PWS53_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0872957.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="PWS53_002784"
     CDS             complement(2168..2527)
                     /locus_tag="PWS53_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0872958.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT         1032 a          471 c          564 g         1092 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatatcta tttctcttag gagtatgttt agatgtttga
     2821 ataatagttg cccaacttta aaggttattc ttataatatt ttacttcatt ttccaataga
     2881 aaattcaaag gattactggt ttttctaaaa tagtttagtt taaatacata agtatcctcc
     2941 gacactgtat tagttcataa tcatctaaat acaaaatttt caaataaaaa tatttcaagc
     3001 tctatcgttg acgaatgtaa acgatactta taaagtaata gaaattgatt acatttgtaa
     3061 acttaaaatg gttaatttat ctcacataaa actaagaata aaataatgat taaaagtggt
     3121 tagtttttat acgatttagc ctatcggtaa aaatgaatt
//
LOCUS       DANJJH010000165         3128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000165 DANJJH010000000
VERSION     DANJJH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            103..1347
                     /locus_tag="PWS53_002785"
                     /pseudo
     CDS             103..1347
                     /locus_tag="PWS53_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="PWS53_002786"
     CDS             1360..1992
                     /locus_tag="PWS53_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0872959.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="PWS53_002787"
     CDS             2197..2424
                     /locus_tag="PWS53_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL0872960.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="PWS53_002788"
     CDS             2493..2759
                     /locus_tag="PWS53_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0872961.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="PWS53_002789"
     CDS             2749..3018
                     /locus_tag="PWS53_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0872962.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DANJJH010000166         3070 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000166 DANJJH010000000
VERSION     DANJJH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..153)
                     /locus_tag="PWS53_002790"
     CDS             complement(<1..153)
                     /locus_tag="PWS53_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0872963.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="PWS53_002791"
     CDS             complement(475..696)
                     /locus_tag="PWS53_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872964.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="PWS53_002792"
     CDS             complement(794..1039)
                     /locus_tag="PWS53_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872965.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="PWS53_002793"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="PWS53_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..2640)
                     /locus_tag="PWS53_002794"
                     /pseudo
     CDS             complement(2263..2640)
                     /locus_tag="PWS53_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
     gene            2716..3070
                     /locus_tag="PWS53_002795"
                     /pseudo
     CDS             2716..3070
                     /locus_tag="PWS53_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1061 a          547 c          442 g         1020 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa taaaaaacac
     2701 actggaggat ttttcatgcc aagacgtcaa cgaagaacct attcaaaaga attcaaacaa
     2761 caaatcgtcg atctctatct cgctggtaag cctcgcgcag aaattattcg agagtatgag
     2821 cttacgcctt cttctttcga taaatggatg aagcaagcac aatcaacggg ctcattcaaa
     2881 gaaagagaca acttaacacc agaacaagca gaattgatcg cactaagaaa gaaaaataag
     2941 caactcgaaa tggagaatga tattttaaag caagcggcgc tgatattcgg acgaaaagac
     3001 aagtaattga tgccaacaag cataaatatt ccatatcagc gatgtgcaaa attctaaata
     3061 tttctcgtca
//
LOCUS       DANJJH010000167         3037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000167 DANJJH010000000
VERSION     DANJJH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            208..2268
                     /locus_tag="PWS53_002796"
     CDS             208..2268
                     /locus_tag="PWS53_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL0872966.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2351..2920
                     /locus_tag="PWS53_002797"
     CDS             2351..2920
                     /locus_tag="PWS53_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0872967.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1104 a          542 c          570 g          821 t
ORIGIN      
        1 ctagatataa ctagatataa ctagatatat tacttaacaa aattaaaaag tacataagtt
       61 attattccga ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat
      121 cttcttttga ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta
      181 gacaagagta gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat
      241 aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt
      301 atgatgcgca cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag
      361 gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct
      421 atcactatta ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact
      481 tcctataata aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa
      541 acattatgga catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact
      601 gatcctacaa aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca
      661 gatataaagg acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag
      721 tttgaaagaa agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta
      781 gaaaggctga agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat
      841 gggaatatcg tggatacgac taatacagga acatctacta tttccttgtc ctattttgat
      901 ccaattgctt taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca
      961 gctatcaaag gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt
     1021 gttgaatatg taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac
     1081 gggacagtag atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg
     1141 aaaacatcaa atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca
     1201 tcgactgtca tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca
     1261 catatgagta gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga
     1321 gtgccaaata tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat
     1381 tggatgcaag caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt
     1441 cactcagaac tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt
     1501 tatataccag acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa
     1561 ataaacaaag aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta
     1621 aaggatgtat cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa
     1681 acaggtgatt ttacgattat gtatagttat catgacccgt attcactcca agaactccat
     1741 gctcagtcac tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa
     1801 agacgagaag aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca
     1861 gggaaagtaa aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa
     1921 tctaataaaa cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat
     1981 gaaattttag agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg
     2041 gaagatcaga aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt
     2101 gattccaccg atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca
     2161 cctggtggag gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca
     2221 tgggtatacg ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta
     2281 tctgttatag tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag
     2341 ggatagaaaa atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact
     2401 tcaacaaaat gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa
     2461 agaagatgaa tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga
     2521 tgaagcactt gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag
     2581 gatgtttttt gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa
     2641 aaaatcaaga gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca
     2701 aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca
     2761 tacggacgga ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta
     2821 taaaaaaatg ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta
     2881 tgaaaaagtt atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc
     2941 tcagtattgg gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata
     3001 ctatcggata actaatggca tgccatacat tcaaaaa
//
LOCUS       DANJJH010000168         3013 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000168 DANJJH010000000
VERSION     DANJJH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            177..359
                     /locus_tag="PWS53_002798"
     CDS             177..359
                     /locus_tag="PWS53_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0872968.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="PWS53_002799"
     CDS             462..746
                     /locus_tag="PWS53_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872969.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="PWS53_002800"
     CDS             762..998
                     /locus_tag="PWS53_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872970.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="PWS53_002801"
     CDS             1020..1274
                     /locus_tag="PWS53_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872971.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="PWS53_002802"
     CDS             1356..1541
                     /locus_tag="PWS53_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL0872972.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="PWS53_002803"
     CDS             1608..2003
                     /locus_tag="PWS53_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0872973.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="PWS53_002804"
     CDS             1993..2370
                     /locus_tag="PWS53_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872974.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="PWS53_002805"
     CDS             2392..2766
                     /locus_tag="PWS53_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872975.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DANJJH010000169         2989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000169 DANJJH010000000
VERSION     DANJJH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            157..951
                     /locus_tag="PWS53_002806"
     CDS             157..951
                     /locus_tag="PWS53_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872976.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            941..2983
                     /locus_tag="PWS53_002807"
     CDS             941..2983
                     /locus_tag="PWS53_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872977.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          957 a          482 c          604 g          946 t
ORIGIN      
        1 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
       61 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
      121 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
      181 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
      241 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
      301 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
      361 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
      421 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
      481 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
      541 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
      601 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
      661 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
      721 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
      781 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
      841 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
      901 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
      961 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
     1021 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
     1081 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
     1141 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
     1201 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
     1261 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
     1321 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
     1381 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
     1441 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
     1501 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
     1561 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
     1621 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
     1681 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
     1741 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
     1801 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
     1861 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
     1921 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
     1981 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
     2041 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
     2101 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
     2161 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
     2221 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
     2281 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
     2341 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
     2401 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
     2461 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
     2521 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
     2581 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
     2641 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
     2701 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
     2761 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
     2821 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
     2881 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
     2941 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacg
//
LOCUS       DANJJH010000170         2918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000170 DANJJH010000000
VERSION     DANJJH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(27..869)
                     /locus_tag="PWS53_002808"
     CDS             complement(27..869)
                     /locus_tag="PWS53_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0872978.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(871..1590)
                     /locus_tag="PWS53_002809"
     CDS             complement(871..1590)
                     /locus_tag="PWS53_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0872979.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            2282..2440
                     /locus_tag="PWS53_002810"
     CDS             2282..2440
                     /locus_tag="PWS53_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872980.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            2512..>2918
                     /locus_tag="PWS53_002811"
     CDS             2512..>2918
                     /locus_tag="PWS53_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0872981.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELT"
BASE COUNT         1078 a          490 c          393 g          957 t
ORIGIN      
        1 tcccagtact gctttccatt ttctcatcat aacgatctct ccttctttct taaaaatttt
       61 tgttttggtg agattagaaa actaattaca aataagaatg ccgctgttaa aacaatactt
      121 gatcctgctg ctatgttaaa actataacca ataaacaatc caagcaatga tgaacttgct
      181 ccgactgttg cagaaataaa tatcatagtt ttcaagctat ttgcatataa atatgctgta
      241 gctgctggtg taattaacat tgcaacaatt aatatagttc ccacactttg catagctgtt
      301 actgctacta aagttaatag aaccatcaat agataatgat aaaaactgac attcatccct
      361 attgcttttg ccattaatgg atcaaaagaa gtgattaata attctttaaa aaatagaact
      421 actataacca acactaaaac cgaaacgcca attgtaatcc acttatcaat atcttgtaca
      481 gctaaaatat ttccaaataa aatatgaaat aaatccgttg agctatttgc aactcctata
      541 aggataactc ctagtgctaa aaaagaactg aaagtaatac caattgctgt atctcctttt
      601 attacactat tgctttttat aaaagtaata atgatagaag ctaatagacc aaatacaatc
      661 gcaccaataa aaaaattaat acctaaaata taagaaattg ctactcctgg caaaacagca
      721 tgagaaatag catcacccat aagagacatt cctcttaaaa taataaaaca acctactgtt
      781 ccagaaacaa tccctataac tacagatgta attagagcat tctgtaaaaa gtgaaagtca
      841 tacagaccat taataaaatt ttgaagcata ttattccccc tctccaataa atatagtatc
      901 tccataggct ttttttaaat tttcttcatt aaatacagat tctacacttc cgtgagcaat
      961 tagatttcga ttcaaaagaa taatattatc aaaatacttc tttaccttgc ttaaatcatg
     1021 atgcacaatt agcactgttt ttccctgatg tttcaatttt tttaacgtat ccataattat
     1081 tcgttcacta actaagtcta tccctacaaa tggttcatct aaaaatatga aatccgcatt
     1141 ttgagcaaga catcttgcta aaagtacacg ttgaaattgt cctccagata attctcctat
     1201 ttgatgatta gaatattttc ccatatcgac cttttccaaa gcctttgaaa ctctttgcca
     1261 ttctgcattt ttaattcttt gaaaaacttt cattccggca tatgttccca tagatacaca
     1321 ttcttttact tttattggaa aagtaaaatc aatatcagct ttttgctcta catatgcaat
     1381 cttcaccagt tttttattga tatcttcatt attaatggag acctctccct gatgagggat
     1441 aatatttaac attgctttaa gcaatgtgga ttttccagca ccatttggac caataatacc
     1501 tgttaaagca ccttcttgta tttttaaaga aatctcgttc aatgctaaaa aatcgttata
     1561 acaaacatta agctttttca cttctaacat aactttctcc ttctaaatga taatgattat
     1621 cacgatcata tggtatacaa aaattatatt ttagtcaacc ctaaatttta tagtttagat
     1681 ttttttctgt tgctgaagat tagaatgaca aaactatttg aaactacgcg tatagtaagt
     1741 agctttcaaa atatataaaa aaggagcgtt ctcaaatgag aacatgctcc taaaactact
     1801 ttattggcgt atggaaaaat tatacttgaa gtggatagcg ggttgaataa gttgataagc
     1861 atcaaaacat ttataaaaat tattcaagac tatgtctgtt ccatatacaa atatgaaaat
     1921 cagtaaactc catttaactt ttttcgtagt gtgtcttttt atacatgttt atcgttatta
     1981 atagtgaata ggaaaaaaac tatttttgat cattgacaat taaatacaat ttttgaataa
     2041 cgatccctgc tattcgagct agaaactcaa gtacgccatg acctgattag cgacaaatac
     2101 ttagagattt ctttttgttc tgggaaaaca aaatgccatg acaatagcag caaataatga
     2161 tacttccgta aggctcgccg taaactgggg agaggtgaaa ttcctatgtg cttacccaaa
     2221 gcaattcaaa aatttcataa ctactatttt aggagatgat tttatagaat ccagagtcta
     2281 cctgaataaa aaacaaacct gtgcatttct aggaatcgat aataatactc tagataagtg
     2341 gattcgtata tataatttcc cagtaataag agttggtggt gtagttcgct ttgacgtaga
     2401 agatattaaa gactttatga atacatttaa aataacttga aattttcttc tcgccgcata
     2461 tactaaaatt agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca
     2521 aagctttcaa atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata
     2581 acaggaattg catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa
     2641 gccgaaaatg atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca
     2701 ttagaattaa aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga
     2761 tatgaattag gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat
     2821 acaaaagata tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat
     2881 ctaaatatca atactgagat aatatcagat gaattaac
//
LOCUS       DANJJH010000171         2855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000171 DANJJH010000000
VERSION     DANJJH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(125..862)
                     /gene="erm(B)"
                     /locus_tag="PWS53_002812"
     CDS             complement(125..862)
                     /gene="erm(B)"
                     /locus_tag="PWS53_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0872982.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(987..1070)
                     /locus_tag="PWS53_002813"
     CDS             complement(987..1070)
                     /locus_tag="PWS53_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL0872983.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(1119..1202)
                     /locus_tag="PWS53_002814"
                     /pseudo
     CDS             complement(1119..1202)
                     /locus_tag="PWS53_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            complement(1612..2406)
                     /locus_tag="PWS53_002815"
                     /note="aph(3')-IIIa"
     CDS             complement(1612..2406)
                     /locus_tag="PWS53_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0872984.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2499..2750)
                     /locus_tag="PWS53_002816"
                     /pseudo
     CDS             complement(2499..2750)
                     /locus_tag="PWS53_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          860 a          573 c          458 g          964 t
ORIGIN      
        1 ttttctaatt tcattttgag gtgaattaat tggtagttat tagaggtgcc ctatacctaa
       61 taatttatct acattccctt tagtaacgtg taactttcca aatttacaaa agcgactcat
      121 agaattattt cctcccgtta aataatagat aactattaaa aatagacaat acttgctcat
      181 aagtaacggt acttaaattg tttactttgg cgtgtttcat tgcttgatga aactgatttt
      241 tagtaaacag ttgacgatat tctcgattga cccattttga aacaaagtac gtatatagct
      301 tccaatattt atctggaaca tctgtggtat ggcgggtaag ttttattaag acactgttta
      361 cttttggttt aggatgaaag cattccgctg gcagcttaag caattgctga atcgagactt
      421 gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa ggtacgcttg tagaatcctt
      481 cttcaacaat cagatagatg tcagacgcac ggctttcaaa aaccactttt ttaataattt
      541 gtgtgcttaa atggtaagga atattcccaa caattttata cctctgtttg ttagggaatt
      601 gaaactgtag aatatcttgg tgaattaaag tgacacgagt attcagtttt aatttttctg
      661 acgataagtt gaatagatga ctgtctaatt caatagacgt tacctgttta cttattttag
      721 ccagtttcgt cgttaaatgc cctttacctg ttccaatttc gtaaacggta tcggtttctt
      781 ttaaattcaa ttgttttatt atttggttga gtactttttc actcgttaaa aagttttgag
      841 aatattttat atttttgttc atgttatcac tcctgaagtg attacatcta taaacaaata
      901 cagaagttaa acgatttgtt tgtaatttta gttatctgtt taaaaagtca taagattagt
      961 cactggtagg aattaatcta acgtatttat ttatctgcgt aatcactgtt tttagtctgt
     1021 ttcaaaacag tagatgtttt atctacataa cgcatttgga ataccaacat gacgaatccc
     1081 tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt atacaaaatt
     1141 ttaagataat gcactatcaa cacactctta agtttgcttc taagtcttat ttccataact
     1201 ttagggttaa ccatacgcaa gaccaatcac tctcggacaa tactcatgat tttaatgata
     1261 aaatccatgt taaacccata gataagaaat acacctgcaa taaccgttac ctgtttgtgc
     1321 caatttaaaa atgcaccact tttttataat taaaacggtt gaaaactgta ccactaataa
     1381 ctcacaatag agagatgtca ccgtcaagtt aaatgtacaa aataacagcg aaatttttaa
     1441 atctatttct tatcgataca aattcctcgt aggcgctcgg gacccctatc tagcgaactt
     1501 ttagaaaaga tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta
     1561 gatgtctaaa aagcttgtag ttaaagcttt ttagacatct aaatctaggt actaaaacaa
     1621 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
     1681 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
     1741 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
     1801 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
     1861 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
     1921 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
     1981 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
     2041 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
     2101 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
     2161 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
     2221 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
     2281 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
     2341 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
     2401 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
     2461 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
     2521 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
     2581 ttgaaaccac aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc
     2641 attagtccat gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg
     2701 cctatgcctt gccccctgaa atccttacat acggcgatat cttctatata aaagataacc
     2761 cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa
     2821 aaaaacgcat atagtagtct caaaactccc aagaa
//
LOCUS       DANJJH010000172         2676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000172 DANJJH010000000
VERSION     DANJJH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..1097)
                     /locus_tag="PWS53_002817"
     CDS             complement(<1..1097)
                     /locus_tag="PWS53_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0872985.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="PWS53_002818"
     CDS             complement(1227..2603)
                     /locus_tag="PWS53_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0872986.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..>2676)
                     /locus_tag="PWS53_002819"
     CDS             complement(2603..>2676)
                     /locus_tag="PWS53_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0872987.1"
                     /translation="ADIILELSGKKLKKVNKMNLEVE"
BASE COUNT          820 a          511 c          382 g          963 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcag
//
LOCUS       DANJJH010000173         2535 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000173 DANJJH010000000
VERSION     DANJJH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..174)
                     /locus_tag="PWS53_002820"
     CDS             complement(<1..174)
                     /locus_tag="PWS53_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0872988.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITY"
     gene            complement(168..569)
                     /locus_tag="PWS53_002821"
     CDS             complement(168..569)
                     /locus_tag="PWS53_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0872989.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(741..2534)
                     /gene="uidA"
                     /locus_tag="PWS53_002822"
     CDS             complement(741..2534)
                     /gene="uidA"
                     /locus_tag="PWS53_002822"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL0872990.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          757 a          496 c          333 g          949 t
ORIGIN      
        1 ataggtaata aattgccccg cctcattaaa tccatttgtc aaacataact tagctatata
       61 actatgataa tgtttgagta tcatcttgat tgaaattgga tcttcttcta gagcagattg
      121 gataatggat agaggaacta atggatactg ccgttttttg gggttgtcta ccataagtca
      181 taccccattt ttaattgttc aactgccttc tttcgttgat accagatacc acttgtactc
      241 atattgagga ttttaccgat ttcagtatca ttaaaacctg caaaataata aagtaggata
      301 acctttcttc ttatttccga taaatgatca atggctgccg ataacttttg atctgaaatt
      361 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggagtac
      421 ttaccttcat ttgaattcgt caatggttcc tcattatctg ttatgttaat gtttcgtttt
      481 cgaatagact tagtttcgtt tcgtaatact tttttacagt aggcatcaaa ttgatgttgt
      541 atagatttct cattgtaatt ttgttgcatg ataaatatgc tccttttttc ccctttccac
      601 taatacaaca atcagaaagc gccttttttt cgaaaaaact agaaaaaaat atccttcttt
      661 aaacagaaaa tctttttata aaaaaaagga gttggcaatt aagcagccac tcctttttta
      721 tattccttat aaaaggtaaa ttatttttta taactgaaat ttggtattga tgaccatcgt
      781 ttctttagat agtgagcaat cattttaggc tttctatctc tagtaaagat tcctttctta
      841 tttccttgca ctcttaaaat cccttgactt gtttcaaaat ctgcaaaatt ccacacttgt
      901 tcaccaacaa aattgtcaca tttatctatt acttcgtgat ttgcttcgta ataacccact
      961 tgatactctt ctgtaaacat tgttggtact gtatcatgaa gtccagctac tgtatctgct
     1021 ccatattctg taaacattat tggcgtatcg ggacatcttt ccatccatcc atctagctct
     1081 tttttcaaca gatattttgc atgttttagg tctccacctg aaacatacca accataatag
     1141 cgatttaaac ataaaacatc gacaatatct cctactttac aaatatctgg tgttgacttt
     1201 aagtaagtaa cgattgttac aggtctcttc tgagggtcaa gttctttagt caattcaact
     1261 aaaggttcaa aatattcttt agcgccttca gaatccgaat ctggttcatt tgcaatagac
     1321 cacattacga cactcggatg gttcttgtcg cgttctatca attccttaat cgccattttg
     1381 tgagcatttt ctgtccgtat ttctttccaa gtatcatgtt tttctacgtt gtcatctaat
     1441 aaagtagcca tgaaattcaa atgtaatcct actgcaggta cttcatcaat aactacaata
     1501 ccttctcgat ctgctaagcg cataatttct tctgaataag gataatgtga agtcctaaat
     1561 gaattagcac cgatccattt cattaaatta aaatctttga tatttactgc ttcattcaaa
     1621 ccacgaccat taatatacgt gtcctcatgc ttcccaaatc ccttaaagta aaaaggttta
     1681 ttattaatca aaaacttccc attcgaaacc tgaatactac gaatgccaaa ttcttctgtg
     1741 tacgtatcaa tcactttatt ttcgttaatt aaactaactt tcatactata taagtaagca
     1801 tttagaggct cccaaagtgt tgggttctta agttgaatca ctccttcttt ccccttagct
     1861 tctgctataa gttgagcttc ttcgtcatag attgctatta gaatttctcc ggtccattct
     1921 gtatcgatat taaattctac atttgcatct gaatcaatta tttttgtatt aatagtgaca
     1981 tcctttatat agtctttcgg agttgtatac aatttaacgg gtctattaat gcctgcataa
     2041 ttgaaaaagt caaaattaac caaattttct ttttttatgt ctccatcttc ctgtatcgtt
     2101 tctttcaaaa tccctacagg taaagtgcta tcgtcaataa cattgtttac agccactgta
     2161 attctgttac ttccatcttt tataaactga ttaatttctg attcaaatgg cgtaaaccct
     2221 cctttgtgtt gattaatcaa ttttccattc aaaaatactt ttgcttcatg tgttgcagat
     2281 ccaaaacgaa gaactaatct ttcttctaat aattttttag gaagaatcac ttctttttca
     2341 taccacaccc atccaacatg atttctaatg ttctctgttt caaataaatc attataagaa
     2401 gaaggaactg aaattggata actctctttt tcaaccattg gttctaaagc aagttcttct
     2461 tgtaagtcat catttgtatc taacttaaat ctccaaagac cgcttaaatc aattacttgc
     2521 ctcgtctctg tcata
//
LOCUS       DANJJH010000174         2533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000174 DANJJH010000000
VERSION     DANJJH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..379)
                     /locus_tag="PWS53_002823"
     CDS             complement(<1..379)
                     /locus_tag="PWS53_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL0872991.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="PWS53_002824"
     CDS             598..1968
                     /locus_tag="PWS53_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HDL0872992.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="PWS53_002825"
     CDS             1989..2273
                     /locus_tag="PWS53_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0872993.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DANJJH010000175         2407 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000175 DANJJH010000000
VERSION     DANJJH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            5..1150
                     /locus_tag="PWS53_002826"
     CDS             5..1150
                     /locus_tag="PWS53_002826"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL0872994.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="PWS53_002827"
     CDS             1321..1779
                     /locus_tag="PWS53_002827"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL0872995.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="PWS53_002828"
     CDS             1938..>2407
                     /locus_tag="PWS53_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL0872996.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DANJJH010000176         2392 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000176 DANJJH010000000
VERSION     DANJJH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            209..2071
                     /locus_tag="PWS53_002829"
     CDS             209..2071
                     /locus_tag="PWS53_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0872997.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..>2392
                     /locus_tag="PWS53_002830"
     CDS             2071..>2392
                     /locus_tag="PWS53_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0872998.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          860 a          353 c          466 g          713 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aa
//
LOCUS       DANJJH010000177         2311 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000177 DANJJH010000000
VERSION     DANJJH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(86..340)
                     /locus_tag="PWS53_002831"
     CDS             complement(86..340)
                     /locus_tag="PWS53_002831"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0872999.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="PWS53_002832"
     CDS             complement(451..705)
                     /locus_tag="PWS53_002832"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873000.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="PWS53_002833"
     CDS             complement(898..1173)
                     /locus_tag="PWS53_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL0873001.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="PWS53_002834"
     CDS             complement(1145..2098)
                     /locus_tag="PWS53_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0873002.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          706 a          411 c          333 g          861 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa a
//
LOCUS       DANJJH010000178         2296 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000178 DANJJH010000000
VERSION     DANJJH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            132..1745
                     /locus_tag="PWS53_002835"
     CDS             132..1745
                     /locus_tag="PWS53_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0873003.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          717 a          407 c          447 g          725 t
ORIGIN      
        1 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
       61 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
      121 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      181 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      241 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      301 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      361 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      421 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      481 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      541 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      601 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      661 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      721 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      781 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      841 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      901 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      961 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
     1021 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1081 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1141 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1201 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1261 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1321 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1381 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1441 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1501 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1561 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1621 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1681 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1741 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1801 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1861 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1921 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1981 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     2041 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2101 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2161 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2221 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2281 gttcctattt atttaa
//
LOCUS       DANJJH010000179         2268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000179 DANJJH010000000
VERSION     DANJJH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            367..1860
                     /locus_tag="PWS53_002836"
     CDS             367..1860
                     /locus_tag="PWS53_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL0873004.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            1995..>2268
                     /locus_tag="PWS53_002837"
     CDS             1995..>2268
                     /locus_tag="PWS53_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL0873005.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT          772 a          358 c          464 g          674 t
ORIGIN      
        1 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
       61 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
      121 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
      181 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
      241 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
      301 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
      361 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
      421 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
      481 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
      541 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
      601 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
      661 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
      721 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
      781 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
      841 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
      901 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
      961 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     1021 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     1081 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     1141 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     1201 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     1261 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     1321 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     1381 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     1441 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     1501 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     1561 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     1621 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     1681 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     1741 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     1801 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     1861 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     1921 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     1981 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     2041 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     2101 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     2161 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     2221 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DANJJH010000180         2242 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000180 DANJJH010000000
VERSION     DANJJH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            42..560
                     /locus_tag="PWS53_002838"
     CDS             42..560
                     /locus_tag="PWS53_002838"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0873006.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            688..1371
                     /locus_tag="PWS53_002839"
     CDS             688..1371
                     /locus_tag="PWS53_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL0873007.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1680..1753
                     /locus_tag="PWS53_002840"
     tRNA            1680..1753
                     /locus_tag="PWS53_002840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1714..1716,aa:Arg,seq:tct)
     gene            1912..>2242
                     /locus_tag="PWS53_002841"
     CDS             1912..>2242
                     /locus_tag="PWS53_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HDL0873008.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLA"
BASE COUNT          754 a          346 c          456 g          686 t
ORIGIN      
        1 tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat tacaaaaaaa
       61 taacttttat aagttctata acgttgatta tatttttaat tcttattctt ctcgtcgtct
      121 tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa atgagtccat
      181 attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag attttagtcg
      241 gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag
      301 ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact tatattgagc
      361 ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt ccatatattg
      421 gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt acgattgctt
      481 tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag aagcggagac
      541 gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag atccaacgac
      601 tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag tcagttgttc
      661 gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc
      721 aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc
      781 aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga aacagttgta
      841 tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat cggagcagct
      901 atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca tgttacgaca
      961 attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg tctaggactg
     1021 acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga gttgtctggt
     1081 ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt tttacctgaa
     1141 tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt ccaaggagga
     1201 acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca tcgtaaatta
     1261 aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat cttgccatta
     1321 ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt
     1381 atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga tgtaaaagtt
     1441 tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga gtagcatgaa
     1501 gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag aatgtctcgt
     1561 tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa ttaaataact
     1621 atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg ctgattcttg
     1681 tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg gggttcgaat
     1741 ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt ttctttgttt
     1801 tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc cttatcgagt
     1861 acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga tatgacgata
     1921 ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat gtatatcgaa
     1981 acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa agaagagtta
     2041 agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg actattagga
     2101 gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac aagtatgcta
     2161 ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg ttggatttta
     2221 ctcaaacaag gtgggctagc tc
//
LOCUS       DANJJH010000181         2222 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000181 DANJJH010000000
VERSION     DANJJH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..544
                     /locus_tag="PWS53_002842"
     CDS             <1..544
                     /locus_tag="PWS53_002842"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0873009.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="PWS53_002843"
     CDS             complement(900..1472)
                     /locus_tag="PWS53_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0873010.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="PWS53_002844"
     CDS             complement(1488..2093)
                     /locus_tag="PWS53_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL0873011.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          698 a          451 c          336 g          737 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tt
//
LOCUS       DANJJH010000182         2170 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000182 DANJJH010000000
VERSION     DANJJH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..914)
                     /locus_tag="PWS53_002845"
     CDS             complement(<1..914)
                     /locus_tag="PWS53_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL0873012.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="PWS53_002846"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="PWS53_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL0873013.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..>2170
                     /locus_tag="PWS53_002847"
     CDS             2006..>2170
                     /locus_tag="PWS53_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873014.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFH"
BASE COUNT          735 a          382 c          417 g          636 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat
//
LOCUS       DANJJH010000183         2103 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000183 DANJJH010000000
VERSION     DANJJH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            75..329
                     /locus_tag="PWS53_002848"
                     /pseudo
     CDS             75..329
                     /locus_tag="PWS53_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            399..911
                     /locus_tag="PWS53_002849"
     CDS             399..911
                     /locus_tag="PWS53_002849"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873015.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            908..1945
                     /locus_tag="PWS53_002850"
     CDS             908..1945
                     /locus_tag="PWS53_002850"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0873016.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          760 a          395 c          434 g          514 t
ORIGIN      
        1 tattcttcaa gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta
       61 aggagtcgct ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt
      121 acacaaaaaa accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta
      181 atccgaaatt gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta
      241 aaaagctatc cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa
      301 caaaatttaa agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat
      361 aataagttat aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac
      421 gttactctac cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg
      481 ggattcgcca attaagtcaa actttgggca ttccagaagc gactctttat tcttggcgta
      541 aaaaagctag aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt
      601 caagtgaaga taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg
      661 cggaatattg ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt
      721 gcttatctgc gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag
      781 aagagaaaaa acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag
      841 cggaagccgc tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag
      901 aggaagaatg atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa
      961 tcgcaatggg gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta
     1021 cgaacgatgg acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc
     1081 tgtacccaaa aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa
     1141 aaaagaattt atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat
     1201 ttatattgca tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca
     1261 ccgtggccga agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc
     1321 aaatgaagtt tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta
     1381 ccggttatat ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga
     1441 atcagaagaa gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat
     1501 ccaaggacag ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt
     1561 tcaagtctta ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa
     1621 tgacaatcct tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa
     1681 taacggcttt aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta
     1741 taacgattct catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg
     1801 acgacatctc gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca
     1861 tcctgaacgc tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa
     1921 tcctttaaaa gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc
     1981 tcgtcaccca ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc
     2041 atagggaggt tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa
     2101 cac
//
LOCUS       DANJJH010000184         2088 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000184 DANJJH010000000
VERSION     DANJJH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..905
                     /locus_tag="PWS53_002851"
     CDS             <1..905
                     /locus_tag="PWS53_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL0873017.1"
                     /translation="FMDNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCA
                     TLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSF
                     EKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGM
                     QNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRS
                     DGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKK
                     GHIKWLNEDIEIDILDEYKHDILI"
     gene            961..1179
                     /locus_tag="PWS53_002852"
     CDS             961..1179
                     /locus_tag="PWS53_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873018.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1250..2065
                     /locus_tag="PWS53_002853"
     CDS             1250..2065
                     /locus_tag="PWS53_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873019.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
BASE COUNT          798 a          242 c          366 g          682 t
ORIGIN      
        1 gttttatgga taatgagttg actgatgatg aactgttaga acttacaaaa gatatatgta
       61 atttaaagcc atataatatt tgtttctgtg gaggagaaac tttactgaga aaaaaaatag
      121 ttttatcttg tgccacttta ttaaaagagg caggtattcc taatattgct attgttacta
      181 atggatcatt agtaactcag gatgttgcaa aagaattatt caaagcaggt attaatcgat
      241 ttcaagtaag tttagatgga aaaaatgcta gtagccatga agcacttaga ttatatccaa
      301 acagttttga aaaggcaatt aatgctatca aattatttag agaattgtcc gaagaagtgg
      361 atgttgcttt ttgtcctact agttttaatt acaatcaatt acgagaagta tatcaactag
      421 taaatactct taaagtgaag tctttaagaa ttcaaccatt tatgcttagt ggtaggggaa
      481 tgcaaaatag ggaaatagtg ccaactgttt ctcaatatag aaaactagtt tctgatatta
      541 actatattaa aagaaatgaa gattatacca agatagattg gggagatcca attgatcata
      601 tcatccgaca cagaacttta ttgaaagatt tttcgaattt tgtttctatt aggtcagacg
      661 gaggattaat ggcatcacct tacttgccaa ttcaagttgg aaatattaaa tgtcacagtc
      721 tttctaaata ttggcgagca ggactttata aagttttttc tttagattta gttcaagata
      781 tagctactca agtttcttca atacaagata tgagtaaaga attaaaaaaa ggacatatta
      841 aatggcttaa tgaagatata gaaattgata ttctcgatga atacaaacat gatattttaa
      901 tttaggttta tcattggaaa gtttagataa acaaagtact tttagaaagg agggaaaaaa
      961 atgatttctg aacctcaaat gaataatatg tatgaaagcc caactataat gggagctggt
     1021 gaagctgcgc caagagcttg ttcagcaatt atggtgtgtg tgttattagc atctgcagga
     1081 gtaggtatcc aagcaggagc ggtgtatact tatgctgtag ttgctacagt agctgtagct
     1141 gcagccgcag tttggaaaac agtaggatta gttggttagg aattaatagg agactaactg
     1201 tctcctattg attccaatga ttaagctaag gaggaatttt aagaaaataa tgaaatacaa
     1261 taatttaaaa ccgaaattac ttaatgagaa catagaatat ataagagaag aaaattatgg
     1321 atatttaacc atagttccca aaaatggaac tgaattgaaa gataacgttt tcaatccaac
     1381 agtaagatat atattggcta actgtactgg aaaaaacagt gtaaaaaaaa tagcgaatgg
     1441 tttaaaaaag aagtttccta gtttagaata tgaaaaattc tatcaagatg ttgtaggaac
     1501 tattaaattt ttcaaacact ataatattat tgatatagga gattatagta tggaagaaaa
     1561 gaaaatatat aaaattgaac aaaatttaga attagaatta ctaaatgaat cacattatga
     1621 agaaatttat aattttatca cagatcatcc tgaaagtatg tattttcatg aagatattta
     1681 tcatcaagga aatagtaaag aattagaagt tgtccttaga tataatttat tttcgtttac
     1741 gagtgaattt tactttttaa gacataaaga gagaattgta ggaatggttt ctataattgt
     1801 taatccattt caaaacttat cctacagtat agtaaattac ctcatatgtg agcctgcata
     1861 tactacgact attttgacaa cagtattaga aaatattaac gaagtttcaa ttttagaagt
     1921 aaagaagcta gtgattcatt tgctaaattc aacagaagat gaatcactaa tcccctattt
     1981 aaaagattca cattttttta aagaagcggt actatctaaa gaatatagag agcatgatgt
     2041 tttgagatac gcttacattt attaaataaa atttctgata gtatttta
//
LOCUS       DANJJH010000185         2064 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000185 DANJJH010000000
VERSION     DANJJH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(604..894)
                     /locus_tag="PWS53_002854"
     CDS             complement(604..894)
                     /locus_tag="PWS53_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873020.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1102..1794)
                     /locus_tag="PWS53_002855"
     CDS             complement(1102..1794)
                     /locus_tag="PWS53_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0873021.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          580 a          386 c          309 g          789 t
ORIGIN      
        1 gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt
       61 tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga
      121 catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa
      181 tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt
      241 caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg
      301 aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc tctttttatg
      361 gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt
      421 taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata
      481 catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc
      541 atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt
      601 tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata
      661 ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct
      721 attattataa taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt
      781 caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct
      841 ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt
      901 cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc
      961 tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga
     1021 aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt
     1081 aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt
     1141 atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa
     1201 gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc
     1261 tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt
     1321 ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag
     1381 atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg
     1441 tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc
     1501 tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc
     1561 tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaag
     1621 cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc
     1681 aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc
     1741 aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat
     1801 agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag
     1861 cgtttttaaa ttacatgtgt aattttaata taaatactat caaatactca tttaatcctc
     1921 cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta
     1981 aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc
     2041 atttcataga ttactgtatt tatt
//
LOCUS       DANJJH010000186         2059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000186 DANJJH010000000
VERSION     DANJJH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..338)
                     /locus_tag="PWS53_002856"
     CDS             complement(<1..338)
                     /locus_tag="PWS53_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0873022.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(405..560)
                     /locus_tag="PWS53_002857"
     CDS             complement(405..560)
                     /locus_tag="PWS53_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0873023.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1177..1398
                     /locus_tag="PWS53_002858"
     CDS             1177..1398
                     /locus_tag="PWS53_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873024.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1399..1656
                     /locus_tag="PWS53_002859"
     CDS             1399..1656
                     /locus_tag="PWS53_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873025.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1792..>2059)
                     /locus_tag="PWS53_002860"
     CDS             complement(1792..>2059)
                     /locus_tag="PWS53_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0873026.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          463 c          314 g          604 t
ORIGIN      
        1 cttttagcat attgaaattt ttctggatta tccatatgag caaaatatcg caccatacct
       61 tttgcattag cacatttttg cgggtttgga gaccgaagct tttgagttat ctcacgtatt
      121 tgatcatacg tctttttacc gctaaacatt atcaaaacat gccaatgagg ctttttcaat
      181 tcaccgtcag gattcacatc tttatcatga agtggactct caacccaagg aacatgtaac
      241 tcatctaaaa actccctcca attttcagga gccgattcag gatagactac aaacgtccaa
      301 ttccttgtgc gttcatcttt agtttttcta ctcgtcatgc tataattacc ttaattgaac
      361 aacagtctaa cagcaaaagc agtgcgccaa cactaatgct tttgctattt tttttgctca
      421 ttttcctttc cgttagcatt aacccaaatt gtaataagac cagacttact aagacccatt
      481 tgtttcgcaa ttttatcaag attatctgcc acttccttag taagcgttac aacgactctc
      541 tttttttcat tactgatcat aattcgtatc ctccaacgac tttttcttaa aaaccatagt
      601 aacaaaaaag taactaaggt gcaacagttt agatactaat tagcgaataa taggccttaa
      661 acccttggca ttactgattt tgtcaaaaaa aaacgctgaa acccttggga gagtaaggct
      721 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      781 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      841 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      901 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      961 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
     1021 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1081 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1141 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1201 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1261 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1321 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1381 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1441 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1501 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1561 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1621 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1681 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1741 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1801 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1861 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1921 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1981 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     2041 atgagcgata atatcactt
//
LOCUS       DANJJH010000187         2040 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000187 DANJJH010000000
VERSION     DANJJH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1872
                     /locus_tag="PWS53_002861"
     CDS             <1..1872
                     /locus_tag="PWS53_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL0873027.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DANJJH010000188         2033 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000188 DANJJH010000000
VERSION     DANJJH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            498..1733
                     /locus_tag="PWS53_002862"
     CDS             498..1733
                     /locus_tag="PWS53_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873028.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1817..>2033
                     /locus_tag="PWS53_002863"
     CDS             1817..>2033
                     /locus_tag="PWS53_002863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873029.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYF"
BASE COUNT          709 a          195 c          353 g          776 t
ORIGIN      
        1 gagcgtaaaa acggctattt ttgccttaac aacgattttt ttgtatacaa atacgatatg
       61 ctcattaatt attggaattt gtagtgctgt ttaaattaat gaaggtgtaa ttatatgagt
      121 gatcattttg aaaaagtttg ttgagaagat gtgcttctag tcatgtgatg ggagaaaata
      181 atttaactgt tagagtgaat agttaaatta ttctggttat atgaaaaaag tagtttctaa
      241 aataatcttg cagggattaa cttaaagaaa ttgagagaat agtttctttg aaatttctat
      301 atacaatgag aagctgaaat ttacttttat ttgatgactg gaaatagaaa ttaaatatat
      361 tgtattgtta cattaaagaa aacttgtatg gaaaaatcag tacaatacca ttatttaatg
      421 ttcttcactt aaagcgcaaa aaatatcagt ttattaaact cgtatataga acaaatatat
      481 gtagacgggg cgaaacgatg agtgtaaaaa aaatgctatt tatggtaagt acaataattg
      541 tattaacatc ccgattttgg ggaattaata ctagtttatc tatgcgatat tttattatgg
      601 caatatggga tttatatttt atgttaagct atttttctta tcgtgagaaa acatataaaa
      661 aatacgttat caagaataac ttttggttga ctataatgcc gtttgtgata ttttctatct
      721 atactctaat aatatgggga attggttcaa atgcagaatt tgaaaatttt ataaagttat
      781 gttccacgtt gctttattta attcttgcat atggttacgc atgtacagca ttttacttat
      841 ttaaggcaga aggaattgat tttatttttt ggtcaggtgt tatttcatat atgagtggtt
      901 cagtttttta tttaattgta tcttatggtt taaatggttt aatttcttat ataaaaagtc
      961 ttataacagg tgaaacaaat actgcaaatt ttgctatgga ggtacatgat ttaacttttg
     1021 cgatgggggt ttttttcctt tattacgtat tttttgaaaa taaaaataca aaaaaacata
     1081 agataaaaat tatcgtatct ttaattttaa ttttcatggg attgaagaga attgaactgt
     1141 tagctttaag tggtgcaatt ctagtgtatt atgttttatt aaagtgggga aaaaagattc
     1201 aaatccgtgc attagtgtgt actatttgtg caactgttat ttcgttagtg tttatttaca
     1261 ttatagatac aggaattttg gcattgctta tgtcaaattt gggcattaca gatgacggtg
     1321 cgaggctttc attttatcta tattcatcta aattttatga acttggttta ggatatgtag
     1381 gaacaggatg gacatggttt tcaaaatact tttttaatct ttatttatcg cactttaggt
     1441 taaatggtgg aagaattgca gcaagcctac acagtgatat attaactatt tatatagaag
     1501 ttggatgtat agtttttatt atatggatgt tttggttatt ttttgtgaaa ccgataagac
     1561 taactaaaaa atttggattg aaaacagggg agtgtgtatt attattaact atatatatgt
     1621 ttatattata tttgacggat aatacgctca cttatccaga cactcaaatg atttttctct
     1681 tagtacctat gatagtttcg tgggttgata atcaacaacg aatatcggaa tagatagcta
     1741 aagtatattt taattatgaa tttataatta gttgtaaaag ttaatattta acggacagga
     1801 agtagggtga aaaaatttga aaaaaatttt agtgttagca aatacatatt atcaaataat
     1861 agtagcaata caaatgaaaa atacaatttt ttccgatgag tatgttgaaa ttattatttc
     1921 tgatcattct aaaaacacta aagaaattgc gaaaaaaata gaagaaaaaa aagtgtttga
     1981 tggctgtcat tatttagaaa gcaaatcttt tgatgataat attgggtatt ttc
//
LOCUS       DANJJH010000189         2003 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000189 DANJJH010000000
VERSION     DANJJH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..378
                     /locus_tag="PWS53_002864"
     CDS             <1..378
                     /locus_tag="PWS53_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL0873030.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PWS53_002865"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PWS53_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0873031.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="PWS53_002866"
     CDS             1859..>2003
                     /locus_tag="PWS53_002866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873032.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DANJJH010000190         1995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000190 DANJJH010000000
VERSION     DANJJH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..844)
                     /locus_tag="PWS53_002867"
     CDS             complement(<1..844)
                     /locus_tag="PWS53_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0873033.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYE"
     gene            complement(863..1267)
                     /locus_tag="PWS53_002868"
     CDS             complement(863..1267)
                     /locus_tag="PWS53_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL0873034.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(1499..1756)
                     /locus_tag="PWS53_002869"
     CDS             complement(1499..1756)
                     /locus_tag="PWS53_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL0873035.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          606 a          393 c          279 g          717 t
ORIGIN      
        1 tttcataaag agaacgactg tctaatgttg gaagtgttag ctttacaggt attcttctca
       61 acaaagttga taatagttta tcctcaggtg gttctgatgt tgcaaatata atccagcaac
      121 ttgatgtgta ccagttttca ttatcaccca tccgatgaaa tttcctcgta tccataaact
      181 gaaaaagttt ctcctgacat tcattgctca atgcatgaat ttcatctaaa aataagactc
      241 cattattagc taattctgct aaaccctttc ttccatcttc ggctcctgta tatgcacctt
      301 ttacatgccc aaaaaaattg gacaagaaaa gttctggatt attggcatat tcactacaat
      361 taactgaaat gaaagatgcc ttctcttcta tataattatt ggccaacaaa tccttataca
      421 cataatttgc taaatagctt tttccagttc cagtctcgcc atgaagtaaa atgggtaagc
      481 catttggcgg atagtagatt gctgctttaa tttgagcaat tacatttcgc agacttcctt
      541 gacttccgat aaccctttct attccaaccc taaaagagct atcataccat ttttccgctt
      601 cctctgaaga gctaaaaatt tgttgatttg gaactttctc cggtaaagaa aagaagatta
      661 ctggacgagt attgattttt ccaaattctc catcttttac gaattcattc aaatattgag
      721 agacaatatt acgactaaca tttaattttt cagatatttt ctgtgctgta ttgttccccc
      781 atttattttt aatctcagag ttctctatga cataattgac aatgcgttct tttaattctt
      841 ccatcacagc acctcctcga cactattcga attttgccga cagtattttt ccataaatat
      901 gacttgctaa aaaggctttg tttagatcat tcaattggaa cgttgcttgt tttgcttgat
      961 ccgctaagac gttgaaaaga tactctggcg aaatcgcata ccccaaaata tcaacgattg
     1021 ttgcaatcag atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacatcgttt
     1081 gtttgcttac tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca
     1141 acaaacggtc ggataaatat ttatagaaaa tcagccccaa taaataattt ttatattctg
     1201 acgcatccat tttactacgg agattatctg ccgcactaaa taacttctga tttaattctg
     1261 cacccaaaaa gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag
     1321 catactcagt tttagaaaaa gcaaaaaaat aaagaaactt attcttgata acaagctacg
     1381 tgacgtatat ccctaacaca aaaatagcca tatcctttgg tttggatacg gctatttgat
     1441 attactcatt caattatcaa atcacagtag gtgtgaatct catcgggatc ggcaatatct
     1501 acttctgatt aggatcaaag gtgattttct caataaaggt gtgatacaaa gctttaactt
     1561 gcttcttctc actctggata agaaagcaat ccaaactagt caatatctgt tggacatctt
     1621 tgacttgaat cggttcccct tgttgttgaa agatccctaa gatttcattc tctcgaattt
     1681 gtaactgtct tcgtttttca gtcaattcat ttaaacgatc ttttaacatc ggaaacaatt
     1741 cgggactatc ttccaaagca gcttcctgtt tttgctctaa tagttagatt ttgcgtacca
     1801 ttttcttctt ttattttcct tcgattgctg attttaatat agtctaaagg aaatttatat
     1861 ttattgtatt ctttataact tcaagagaac ttcgtttgag tattactgta tttttttaat
     1921 tccataagaa aattagttat tacctgccac ctaatcagca aatctgaacc tgagtcaata
     1981 taattaatat tattt
//
LOCUS       DANJJH010000191         1992 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000191 DANJJH010000000
VERSION     DANJJH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="PWS53_002870"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="PWS53_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0873036.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="PWS53_002871"
     CDS             853..1041
                     /locus_tag="PWS53_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0873037.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="PWS53_002872"
     CDS             1054..1611
                     /locus_tag="PWS53_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0873038.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="PWS53_002873"
     CDS             1643..1885
                     /locus_tag="PWS53_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0873039.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          553 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cc
//
LOCUS       DANJJH010000192         1921 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000192 DANJJH010000000
VERSION     DANJJH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..177
                     /locus_tag="PWS53_002874"
     CDS             <1..177
                     /locus_tag="PWS53_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873040.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="PWS53_002875"
     CDS             190..690
                     /locus_tag="PWS53_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0873041.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>1921
                     /locus_tag="PWS53_002876"
     CDS             787..>1921
                     /locus_tag="PWS53_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL0873042.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGT"
BASE COUNT          604 a          264 c          439 g          614 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 a
//
LOCUS       DANJJH010000193         1894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000193 DANJJH010000000
VERSION     DANJJH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            21..1190
                     /locus_tag="PWS53_002877"
     CDS             21..1190
                     /locus_tag="PWS53_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0873043.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(1457..>1894)
                     /locus_tag="PWS53_002878"
     CDS             complement(1457..>1894)
                     /locus_tag="PWS53_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873044.1"
                     /translation="GTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVI
                     YSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSES
                     GINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          668 a          325 c          293 g          608 t
ORIGIN      
        1 aggagactaa ataaactatt atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat
       61 ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt ctctacagga actattcttg
      121 gatggcaatt ttacaataat gaacactctc agatagttgc ccatgctcaa tcagctcaaa
      181 aaaagttgat actgactacc aatgaacaac agctcgttga aaaaatcgat caactattaa
      241 gaaaaaatga ttatattggc tcaatttacg taaagaaaaa taatcgcatc atcatggaaa
      301 aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa tgatccttct ctttattatc
      361 agattggttc ggttcaaaaa gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg
      421 ggcacctttc tttagatact aagctggctc aattctatcc acaaattgct ggcagccagc
      481 aaattactat ccgtgacttg ctgtatatgc gctcaggctt gcaccgaact gcttctccaa
      541 ccataccgat gactgatgaa gaagtcgttc aatttgcgat tcagcactta aaatttacag
      601 attatcagac ctctcattat gattcattga actttaccat attgactgga attttgatca
      661 aattaacaaa tcaaccgtat gaaaaattat tgaccgatga tatcatcgaa cctttaggat
      721 tacagcatac aaaattttat gaccaagtca aaaattcatc caatcatgca ctatcctatc
      781 aaatgtctaa agaggatgat tactttaaac cactgacaga atctgaaaca gagatccata
      841 acgaattagg gactggtaat atcagtatgt ccgtctatga tttaaatacg ctgtttacac
      901 aagttttatc tggaaaacta gtttccaaag acttactctt ctcattatgg cgtgaaaatg
      961 cgaatggaca tccatatagt ggtggggtat atagcggaga taattatatc ctgtcgcaag
     1021 gaaatatcga ctcttttgat acatctgcta tatttagcaa agattcacag gatgctatca
     1081 tcatggaaag caatataaaa gctgacaaaa ctatcaaact gcctactgct gatctaagaa
     1141 accaaatcta tgacctgatg gaaggaacta ctaagttggc aaatagctga tatgatgaaa
     1201 agatagcaaa tactacttct ttgaatagca tttgcttaaa aaaagtactc gactaaacgt
     1261 aggaaagagt gtgggacaaa tctaaaaatc aatttttgct acagacttta attccaaata
     1321 aacagaacag aaagaggtgt tcttctttct gttctgtcca aattattcta agatttctct
     1381 ttaccaacat tatttgacaa agggcctatt aaatctaaaa tagtatcttt agattgctaa
     1441 aatatgtgct agcaaatcat tcatggcttg ttcttatttt taattttatg caataaaaaa
     1501 gaagaacaat tagcgtgatt atagaaataa ataatgagat tttagaaaaa aatgtgtatg
     1561 aatacctgat tgttacttta tttattccac tttcactttt tactgtaaca aaaggacctc
     1621 cttgttttag tactgtagca ttttttgaat taacagtaac cgttaatcct ttataccgga
     1681 atacaggtaa aactattgtt ttgccttttg aagatttaaa cgtaaatgta atttcatctt
     1741 tatttggaga aaagctacta taaataactt gaccatcttc tgtaacttga tggtttttca
     1801 aaagcaattg atctttttta tagcctatag gtacataatc aaaattctca aattcttcaa
     1861 gtttattttc catttgttca ttagaataag ttcc
//
LOCUS       DANJJH010000194         1878 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000194 DANJJH010000000
VERSION     DANJJH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..86
                     /locus_tag="PWS53_002879"
     CDS             <1..86
                     /locus_tag="PWS53_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0873045.1"
                     /translation="FENVVTGKENWNDLYHANEVLKIAEEK"
     gene            87..839
                     /locus_tag="PWS53_002880"
     CDS             87..839
                     /locus_tag="PWS53_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0873046.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            844..1791
                     /locus_tag="PWS53_002881"
     CDS             844..1791
                     /locus_tag="PWS53_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL0873047.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          651 a          258 c          374 g          595 t
ORIGIN      
        1 tctttgaaaa cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag
       61 tattgaaaat agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg
      121 ggtctaaggg gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt
      181 tcgctttagc aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt
      241 gtttgaatac ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga
      301 aaattgtaga acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac
      361 gtgattcatt cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag
      421 atttgacttc acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa
      481 aaaatttaga taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta
      541 atatggtaaa aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta
      601 ggcaacagac acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat
      661 ttctaaaaaa aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt
      721 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
      781 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
      841 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
      901 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
      961 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
     1021 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
     1081 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
     1141 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
     1201 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
     1261 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
     1321 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
     1381 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
     1441 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
     1501 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
     1561 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
     1621 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
     1681 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     1741 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     1801 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     1861 atcaaatctg ggatagaa
//
LOCUS       DANJJH010000195         1837 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000195 DANJJH010000000
VERSION     DANJJH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..741
                     /locus_tag="PWS53_002882"
     CDS             <1..741
                     /locus_tag="PWS53_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL0873048.1"
                     /translation="SKSPYSNCIWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILT
                     EDNYRQYVDIPKWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDE
                     YFDIDIWSIRRPDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVD
                     YLMIDYMIVLAQRINHEIAQTFDKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLF
                     KLTWKQSFKKQKDDKVTFYGMLVENKLL"
     gene            763..1818
                     /locus_tag="PWS53_002883"
     CDS             763..1818
                     /locus_tag="PWS53_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0873049.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          731 a          185 c          352 g          569 t
ORIGIN      
        1 tcaaaatcac catattctaa ctgcatttgg gtttgttggt ggcagggatt ggatcaatcg
       61 ccggaaattg taaaagcttg tgtaaaatca ataaaacgaa atgcagggaa acatactgtt
      121 ataattttaa cagaagataa ttatagacaa tatgttgata taccaaaatg ggtggaagaa
      181 aagaaaaaca aaggaatcat tacgagaaca aattattctg atttattaag attatctttg
      241 ctagcaaaac atggaggttt gtggttagac gccacatttt attgtacaaa accaatacca
      301 gatgagtatt ttgatatcga tatttggtct attaggaggc ctgattatgc tcacgcatcc
      361 gtagcatcag gatattttgc gggttattca ttagcatgta atcaagagtc tagatggatg
      421 tttgctataa ttagagactt ttttttaaat tattggaaaa ataatgatat gatggtggat
      481 tatttgatga tagattatat gattgtttta gcacagagaa taaatcatga aatagcacag
      541 acatttgata aaatacctag taacaatcca ttatgtgacg aattgattaa aaatttaaat
      601 aatatatatg atgaaaaagt atggaactta ttaaagaaag atactttact gtttaagttg
      661 acatggaaac aatcttttaa aaagcaaaaa gatgataaag ttacttttta tgggatgttg
      721 gtagaaaaca aattattata aatattagag gtataaaaag ttatgaaaca caaggtaagt
      781 gtgatagtac caatttacaa tgttgaaaaa tatttaagaa aatgtgtgga tagtttattg
      841 agtcagacat taaaagagat agaaatcgta ttagttgatg atggttcgcc agatgcttcg
      901 ggggaaatag cagatgagta tcaaaaaaaa tattctaatg taaaaacaat acatagagaa
      961 aatggggggt taggaccggc aagaaataca ggaattgaga atgcgacagg agaatatgtg
     1021 gcttttcttg attcagatga ttgggtagaa agtgatatgt atgaaaaatt atacttagtt
     1081 gcgtccaaag aaaatgctga tatagtagtt agtggacatt gtgattttgc gaatgacaaa
     1141 gctatggtga aaaagcaaca tcctttgagt gggaatgtat atgatacgcc taatagtatt
     1201 aaaaatgtac gaaaaaattt atttggacat agtccaaaag atcaagaagt agaggccttc
     1261 ccgatgtcag tatgtatgtc tttgtataat aaaaatctgc tagataaata tagactaaga
     1321 tttgaaaaaa ttttatctga agatacaatt tttaacttga atgcatatga tgtcgcaaat
     1381 aaaattgctt ttacagaata tacagattat tgttatagaa aagaagaaca agagtctatt
     1441 acaaaaagtt tatctccaaa taagcagacg gaatttaaag aatttttaat aactctaaaa
     1501 gctatagcat cgaaagaaga tttagaatgt caaatcagag caaaacgtat ggcaattgat
     1561 tattgtaggc tttatgtggg cttagttgat gatgcaaatt tgggaattaa agagaaaaga
     1621 aagtatataa aaaaatttat tgaagatgag ggattaattc aatgttggaa gggatatcct
     1681 acaaatacgc taccatttca acaaaaattt tttcataaaa tgcttgaatg taagtgttat
     1741 acaatattgt tgttgttaag tagaattaga aagataatta aaaatatgac cagaaaaaaa
     1801 ttgtttataa tattataaac taaaagtata tcaaata
//
LOCUS       DANJJH010000196         1836 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000196 DANJJH010000000
VERSION     DANJJH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..256
                     /locus_tag="PWS53_002884"
     CDS             <1..256
                     /locus_tag="PWS53_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDL0873050.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            354..563
                     /locus_tag="PWS53_002885"
     CDS             354..563
                     /locus_tag="PWS53_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL0873051.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            580..852
                     /locus_tag="PWS53_002886"
     CDS             580..852
                     /locus_tag="PWS53_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0873052.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            854..1717
                     /locus_tag="PWS53_002887"
     CDS             854..1717
                     /locus_tag="PWS53_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDL0873053.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          739 a          290 c          336 g          471 t
ORIGIN      
        1 gatcggtttt gttccttatt tagttgatac ggacagcgca acgataaaat caaacctgga
       61 agaactgtat aagcaacata aagaagataa cttggttttc caaaatatta tcaagcgaag
      121 taataaagta agtacctggt ctaaaaacgg cattacagag cataaaggct atgacaaaaa
      181 agttttatcc atgtataaga acgtattttt tgaaatgctt gagcgaatta ttcaattaga
      241 aaacgaaaaa gaatagaatc acaaatcaca agtgattaat cacaaatcac ttgtgatttg
      301 tgattgttga tgataaaata agagtaagaa gaaatagaaa gaagtgagtg attgtgggaa
      361 atttaggcgc acaaaaagca aaacgaaatg atacaccaat cagtgcaaaa aaagatataa
      421 tgggagataa gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag
      481 caaagaatgg cggaaacgta aaagaagtta tggatcaagc cttagaagaa tatatacgga
      541 aatatttacc tgacaaactt taaaaggagt gaaaatgaaa tggcagttac gtatgaaaaa
      601 acatttgaaa tagagatcat taacgaatta tcggcaagcg tttataatcg agtattaaac
      661 tatgttttga accatgaatt aaataaaaat gactctcaat tattggaagt caatttatta
      721 aaccaattaa agcttgcaaa acgtgtaaat ctttttgatt attctttaga agaattacaa
      781 gccgttcatg agtattggcg gtcaatgaat cgttactcaa aacaagtttt gaataaagag
      841 aaagtggctt aatatggcaa atatagtcaa ttttactgac aaacaatttg agaatcgctt
      901 aaatgataat ttagaagaat tgattcaagg aaaaaaagcg gttgaatcgc caaccgcttt
      961 tttacttggt gggcaaccag ggtcagggaa aaccagtttg cgatcagcaa tttttgaaga
     1021 aacacaaggg aatgttattg tcattgataa tgataccttt aaacaacagc accctaattt
     1081 tgatgaacta gtgaaacttt atgaaaaaga cgtagtaaaa cacgttaccc cttattctaa
     1141 tcgcatgaca gaagcgatca taagccgttt gagcgatcaa gggtataatt tggtgatcga
     1201 aggtacagga cgaacaacag acgttcctat tcaaaccgca acaatgcttc aagccaaagg
     1261 ttatgaaaca aaaatgtatg tcatggcagt acctaaaatc aactcatatt taggaacaat
     1321 tgaacgatat gaaaccatgt atgcagatga tccaatgaca gccagggcaa caccaaaaca
     1381 agcgcatgat attgttgtca aaaacttacc gaccaattta gaaacccttc ataaaacggg
     1441 cttatttagc gatataaggc tatataacag agaaggagta aaactctatt caagtttgga
     1501 aacaccttct attagtccaa aagaaacctt agaaaaagaa ttgaatcgta aagtatcagg
     1561 gaaagaaatt caaccgactt tagaacgaat agagcaaaaa atggttctaa ataaacacca
     1621 agagacacct gaatttaaag caattcaaca aaaattggaa agcttgcagc cacctacacc
     1681 accaataccc aaaacaccta aacttccagg tatttaaacc ttaaaaaagg aaaagagtag
     1741 ttaccaaaaa acggtaacta ctcttttttt ataaaaacat ttcctcatgt tggggtcccg
     1801 agcgcctacg aggaatttgt atcgataaga aataga
//
LOCUS       DANJJH010000197         1799 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000197 DANJJH010000000
VERSION     DANJJH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="PWS53_002888"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="PWS53_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0873054.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DANJJH010000198         1740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000198 DANJJH010000000
VERSION     DANJJH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..177)
                     /locus_tag="PWS53_002889"
     CDS             complement(<1..177)
                     /locus_tag="PWS53_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0873055.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGK"
     gene            complement(174..827)
                     /locus_tag="PWS53_002890"
     CDS             complement(174..827)
                     /locus_tag="PWS53_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0873056.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1115..>1740
                     /locus_tag="PWS53_002891"
     CDS             1115..>1740
                     /locus_tag="PWS53_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873057.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          556 a          317 c          237 g          630 t
ORIGIN      
        1 ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt
       61 ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat
      121 tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt
      181 ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg taattttttt
      241 ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc gctatctttc
      301 catacataat caataatttg cattcttgtt aacacttgtc cattattatc caataaacat
      361 tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta
      421 tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc
      481 ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca
      541 tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc
      601 aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt
      661 ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag
      721 gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt
      781 attccctctc ttatcatatt atcatcttca acagttaata ttttcatata tacctccact
      841 taagcctata cactagatat tatacagcat attatggctc tataatatct agtgttggtg
      901 acaccttctg ttgccaatac tttttctttt ttctaataaa tgcaaacaga caccttgaaa
      961 tcctttagaa taaagggttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc
     1021 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     1081 gaccaaaaca tcaccctacc aaaggagtcg ctctatggat aatcatacta gaaaattact
     1141 cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa
     1201 aggcgttcaa gcgcagttta ttaaagggag attggatgac tcaccttccc attgtgaaca
     1261 ctgtggatcg attcgtatta tccgcaacgg ttcctatacg acacgtactc agatattgaa
     1321 agtcaaagaa aagttgacta ttttagaatt aaaacgtacc cgttttctat gctatgattg
     1381 tgggcagacc ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga
     1441 actgaagcag gcgattctca tagaactgta cgaaaatcaa tcacgtaaac taatcgctaa
     1501 gaagtacttc gtctcggatg ggacggtgac acggatcttg cgtgaagcaa cgaagcatta
     1561 ccagccacgt atgaacttcc ttccgacggt gttgtgtatg gatgagttca aatcaatgaa
     1621 gtctgtttct ggttccatga gttttatttg tgttgatggg acaacccatc agctatttac
     1681 gatccttgaa gatcgcagac tttacaaact aactcaatac ttcctgcgct ttcctagaaa
//
LOCUS       DANJJH010000199         1726 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000199 DANJJH010000000
VERSION     DANJJH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            240..1718
                     /locus_tag="PWS53_002892"
     CDS             240..1718
                     /locus_tag="PWS53_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873058.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          638 a          238 c          304 g          546 t
ORIGIN      
        1 tgtggttgtt tacgcaaatg gcgcaaatct tgtacatttt aacacaataa aacaaaaaat
       61 atagaatatt tcctcataaa tttttccatt ttttgcaata ttctcaaaaa aaagataaaa
      121 aatgagaata actgatgaaa aaacaagcat aatccatcat aaaatcagta taggtgaaaa
      181 gatgaacttt catctgcata tcaagtaatt ggtatgaggc attttttgag gtgtaggata
      241 tgaagttgga ttaccttata gaagaggaag atagagaaaa actgctgcta gctagatatt
      301 tagaatttga gaaaaagaat tatgtagtgc tgcctaagtt atatgaaaca ttaggtttgt
      361 ctaaatttaa aacaagaaat tatatcatag aattaaataa agaatttact gaattcaaaa
      421 caaagccaga gatccaaatt tcagaagaag cagagatcca tgtcgaacgt ctgaatttga
      481 cggatatcaa aaatttaaga agaatctact ttcaacgatc aaaaattgct cgactgttaa
      541 cggagatcgt ggattcaaaa gtaacgatcg aaaaatttgc agagaatcat tttttaagca
      601 tatcaagagc ctatgtaaaa agaagagaat tagtctcttt tcttgaacag catcaaataa
      661 agttaaagaa aaacatatta gttggggaag aaataaacat acggaacgta ttatttgggg
      721 tatatttcgg ggtctttaat ggtttttcta tgccatttag agaaagcgtc atgaatgaag
      781 caaagagtat cgtgcaatat tttgaatatc tgttcaattt aaaactgtct gcaacaaaac
      841 gaatcaaatt caatttgcta accactatta gtttgttgag ggcctatggg aatcaaccaa
      901 tcggaaaacg tttcttttca gatgaatact ttaagagctc agaaggaatg aagatcatca
      961 aggatttatc acatatttct tttcttcata tccctaaaga gcagatcatt gatgagattt
     1021 cttatatcat gttgtttttg atcacggaag aagaatttga attagatggc gcatttggac
     1081 ttgcggaagc tcattttcat gatatccaca tgattagtca aaaaatgtca gtggaattga
     1141 ttgatcaatt accattttcg attgaaatct ctgaagaaga aagactatat atacgaaaag
     1201 aacttcgtat gggtatgatg catgtcaatc gaaaaaattg gatattcgac tttgagatga
     1261 ctagctttac gtctaaaaag cagctacagt ttttcttcga gacgtatcca atattcagta
     1321 cagcaatcca aaaggtcata caagagaata aacgctattt cttgggaaaa gatgaacgtt
     1381 tcatgattcg cttattttat gattatctct ttttattggt cggtatttgt ccggtatcat
     1441 attttgaagc accgattcat gtctgtattg atttttcgca aggatctgct tataccgatt
     1501 acattatttc tcaagtgaaa ggatttaaaa attacaatat tgtgattgag agtcgaatca
     1561 cttctcgtac gaatatttat ttatctgatt gtttacttga aaaatttaat aaagaacaga
     1621 taatttggaa aaatccaccg actccagatg actgggaata ttttggaaat accatcatca
     1681 gagaaaaaaa tagacattta actgaaaaaa tcagctagta tttatg
//
LOCUS       DANJJH010000200         1708 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000200 DANJJH010000000
VERSION     DANJJH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..208)
                     /locus_tag="PWS53_002893"
     CDS             complement(<1..208)
                     /locus_tag="PWS53_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873059.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFK"
     gene            complement(210..1637)
                     /locus_tag="PWS53_002894"
     CDS             complement(210..1637)
                     /locus_tag="PWS53_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL0873060.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          669 a          287 c          194 g          558 t
ORIGIN      
        1 gtttaaagga agaaccgatt ttccggtgat tcacaaaaac aacatcatta aatagcccta
       61 attcttttat acgctcacta tatcttctag cgttttcaaa ttcagaataa ataaataaat
      121 ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag
      181 gagtccaagc aatgaaagct gtcttcatat taattctcct tttttaaata caagtaagaa
      241 atgcttccca aataatacca gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca
      301 tgagcctcca ccatttcatc tataaaaata gatagtataa aaattaaaac aataccaaga
      361 aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat atttaacaca
      421 tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac ttgaagaaat
      481 ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt atcaagatct
      541 acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc ataaccaaat
      601 aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg accagctgaa
      661 atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag tggagtttta
      721 tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg aattgttgct
      781 aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac taataatatg
      841 cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct aaattttgaa
      901 ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg ggcacctcta
      961 gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata aaaaacacga
     1021 atcgtttttg ttatttttga aattaataca ctaattaaac ttaaaataat agaagtatat
     1081 ccaaacatag ctccaacatt tggacttgta tagacaccaa ataaccgatg ttcaataaaa
     1141 ccttgtcttg caagtaatcc tggcgttcct gttgggacat gataagaaat caaaaaaata
     1201 aattgataga tagatattag cgcagctaaa aaaattatga taatgaaaac attattaaaa
     1261 tacaccattt ttttcttttt ttcctcttca ctttgtgaaa agtctgcagg aaaaattact
     1321 atcaaagtaa tcagcaaata tccaaaatta taaatagatg gaattaaaaa attttctctg
     1381 ttaatgatta tagttaagat aaaactaatc attgctccaa tcagtagcca agaaaaacgc
     1441 attttaaaaa aatttttctt tataaaaacc tcatacaaaa ctataattcc accccaagca
     1501 aaagctattt tcatcgggat attagttaca ttaaaagtac taggcataaa ataaagcaga
     1561 caaatgatta aatttattac tcgaaacgca tctcgactta ataaactctt ctctaaaaca
     1621 ggtactattc tattcatcta tatttccaac tttcaaaatt tctttatcaa ttctatttaa
     1681 tagtttatca gagtaatccg atttaact
//
LOCUS       DANJJH010000201         1621 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000201 DANJJH010000000
VERSION     DANJJH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(30..1061)
                     /locus_tag="PWS53_002895"
     CDS             complement(30..1061)
                     /locus_tag="PWS53_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0873061.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          504 a          250 c          274 g          593 t
ORIGIN      
        1 tattgtgaaa aaaagatcat acatttgttt tatatggaag aaacccgaac gaatgccgct
       61 aattcgtgat aagaaaaatc aaccatcttt tcccagatat cttgaaaaga atgggatgag
      121 aggtatcgat taccacataa ataatttacc gcgtcagcat catctaaata tagttctgca
      181 taataacgat atgtttttct gcatagttct ggcgatacgt ttttatatac tgtaattttg
      241 ctcaaaattt ggcgaatagt ccgaggagaa atttgctgat catgtgagga acgaaaaaca
      301 aaagaatttt cgtccgtatt ttgtgctgtt aacttaaaat agttctccag aaatggcatc
      361 gcttcttttg gtattggtac tttattcagt ctgttttctt ttctattcag attaagaatc
      421 tgcttggtca aagatacatc tttacgttta agttctacta cttcatggat ctttaatccg
      481 caatacagaa gaatggcagt aattgcaagg ttcctcggtc tattcttttc ttcaaagcta
      541 gcttctctgc ttgtttgaaa gccttcaatc gggataaaca aatgctcaaa atctttgaaa
      601 agttctgcat cgatatatcg cggagctgct ttggttgttt ccggtaaact tgtacccaaa
      661 agagaaatct cttcttcaat cagtcctttt tgatacaaaa atcgtaaaaa atgatttaat
      721 gaatattgct tccttgaaat cccgtaatag ctattttcaa acaattgctc cgtttcttgg
      781 ttcgctgtag tttggaacga acgagtatgt cgatagattt tagctaagaa attctgataa
      841 tcttgttttt ctaaatggat aatcagctga tcatccactt cttcagaaaa tacttttctc
      901 aaataatcag taaacagatg aaaatctttt gcgtattcat agcgactgcc taatgaaagg
      961 gaagaacgat tttcttctaa aaactgttga aagacggaag ggagcaagtg tgtacgttgc
     1021 tttaactttt cttgcagttg ttttctagtt ttgctgtcca ttacaaattc cctccaatag
     1081 tgttcatttt ctatatttta ccgcatttca tcaaatgaag atagacagtt tgtcttgatc
     1141 ttaggaagca aatttttaac taagtactag ctaaaagata tgaagtaaga agatacattt
     1201 ttagttattt tattagtgca gtcattcttt tgatctttta attgatttat cctcttttta
     1261 gctatgtggt tacttcatga gcaatgaaat ggatttggtt cgttctaatg gcaggaggct
     1321 ctttctttta tttccgaaaa attagaaaac attttttcag aatcgattgg ctctccattt
     1381 attcaataat ggtttgtcta acgatattgt tgggataatt cttaaagaag tattagaaat
     1441 agttggtact tcattacttt acatgaagat ttattctatg gtaggagcaa ttatattgag
     1501 aattaatcct tttttatata agttgtttgc ttattgatgg attggataaa ttaaaccatc
     1561 acaacgagtg gaaattatat gaaatttttc ataaaaattc atatagaaac aaatgacatt
     1621 t
//
LOCUS       DANJJH010000202         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000202 DANJJH010000000
VERSION     DANJJH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            202..1077
                     /locus_tag="PWS53_002896"
     CDS             202..1077
                     /locus_tag="PWS53_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873062.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="PWS53_002897"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="PWS53_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT          570 a          284 c          248 g          471 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tcc
//
LOCUS       DANJJH010000203         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000203 DANJJH010000000
VERSION     DANJJH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANJJH010000204         1543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000204 DANJJH010000000
VERSION     DANJJH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            191..445
                     /locus_tag="PWS53_002898"
     CDS             191..445
                     /locus_tag="PWS53_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0873063.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="PWS53_002899"
     CDS             446..763
                     /locus_tag="PWS53_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0873064.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1543)
                     /locus_tag="PWS53_002900"
     CDS             complement(1397..>1543)
                     /locus_tag="PWS53_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL0873065.1"
                     /translation="EKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRY
                     SLGY"
BASE COUNT          546 a          231 c          290 g          476 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttc
//
LOCUS       DANJJH010000205         1510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000205 DANJJH010000000
VERSION     DANJJH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            102..869
                     /locus_tag="PWS53_002901"
     CDS             102..869
                     /locus_tag="PWS53_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0873066.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1061..1459
                     /locus_tag="PWS53_002902"
     CDS             1061..1459
                     /locus_tag="PWS53_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0873067.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          562 a          189 c          282 g          477 t
ORIGIN      
        1 aaatactgtt aaatatctcg aaaaatcaat tacgagttat ttgccttatg tgaaccttta
       61 tgattaacaa ctgaaaaaat gcgtgaagga aggaaaaact tttgaaaaaa gatgaactga
      121 caaagtacaa tgaggagttc ttagcaacaa ttgacatgaa aatggattta aagtcgtatg
      181 aactttatca attgctgttc agttatatct cacccacaaa gctaaagaaa acaggcgtag
      241 tagatttacc tgttaaagcg attattaatt acatggatgt tgatcaaaat cttgaaacgg
      301 aactaaaaac catagttaac actttgcaaa gacaagcagt ttatgaaatt tcgaatctag
      361 acaaaaaata taagacgtat actactgtat ctgctattga tcggattcat tgggatttag
      421 aagagcatag tgtatctttc agtttttcag aagatgtaat tcccttaatt gtctcaatca
      481 aaaagaagta tccgttcttt gtttttgaag acttagcatc ttttcaaaaa aaatatagca
      541 tcgtgttata caaatatttg gcgatgtatt ataaaaaata tcttatcgcc aaaaataaag
      601 gagattttga gaaagctgaa atatataaga atcccctgat tgaaatagtt aaaattcgtg
      661 agattacgaa aacagaaaat gagtataaaa caatatcgaa ttttgataga cgtgttttaa
      721 gggaatcagt tgaggaaatt aatgaaaaat caatgctgaa tattgatttt gctaaagtta
      781 gaaaaggcag aaattacaat tttgctgtcc agtttaatat ttctagtgag tttgatgaaa
      841 aaacttatag agaaaaaaaa ttaggttaat gcaaaatata tcttactcgt ttgaagatgc
      901 ttcagacgta tgctaagata tatagtgcct tgggagcaag gtattaatct cctttagagg
      961 ttaattagct tgtgatcgcc tatcaatttt tttggtaggc gattcttttt tttgaaacaa
     1021 aaaagcaaaa attatttttt aattagagag gtgcaatgcg atgaaatact ttttttgtgc
     1081 agtattcgaa tggaaaggaa atgaatatgc agttaaattc ccagattttc cagacttata
     1141 tacacatggt gaaactttag aagatgcttt tgagatggcg caagaagtat tggaaggata
     1201 cttggtgctt ttagagttgc aagaaaaaga aattccatcg cctacgcctg ttcaagagtt
     1261 aaatgcaaga atcaagcgat ctgagaatgt taatttattt gtcgtaaaag ctaatacgaa
     1321 agttgctaaa gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg tggaacacta
     1381 tttaaatgtt ttagcggctg aagagaatat taatctgtcg aaagctttga atgatttgtt
     1441 gagagaaaag ttgatttgat gtctctcaga tgatcatatt aagcaaaaac agcttgtttt
     1501 aggacgtatg
//
LOCUS       DANJJH010000206         1508 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000206 DANJJH010000000
VERSION     DANJJH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(61..279)
                     /locus_tag="PWS53_002903"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="PWS53_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="PWS53_002904"
     CDS             complement(545..739)
                     /locus_tag="PWS53_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873068.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="PWS53_002905"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="PWS53_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          529 a          234 c          201 g          544 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatc
//
LOCUS       DANJJH010000207         1484 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000207 DANJJH010000000
VERSION     DANJJH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            286..693
                     /locus_tag="PWS53_002906"
     CDS             286..693
                     /locus_tag="PWS53_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873069.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="PWS53_002907"
     CDS             707..1108
                     /locus_tag="PWS53_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873070.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..1481
                     /locus_tag="PWS53_002908"
     CDS             1110..1481
                     /locus_tag="PWS53_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873071.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          472 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aactcaccta atga
//
LOCUS       DANJJH010000208         1472 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000208 DANJJH010000000
VERSION     DANJJH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            278..1318
                     /locus_tag="PWS53_002909"
     CDS             278..1318
                     /locus_tag="PWS53_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0873072.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT          514 a          160 c          331 g          467 t
ORIGIN      
        1 agtttagggg tatatatttt acatgaaaat cattatttca ttgatggtat gaagtctaga
       61 ggcttaatat gggatgggat atttggatta ggcaaaaggt ttttagatag ggatttttgg
      121 atttggttta taattgcagt tgtagttgtg tgcataattt gcatggctat cgatgttgta
      181 agagaaaaat ttgaaagaca gatttttaaa attaagagtg taaagaacct gtgtaaactt
      241 atgaatcatg ttataaatat atacccataa ttaataaatg aggagaaaag aagtgaaagt
      301 tatatcattc ggagaattat tattgagact tgcagcaccc ggttattcaa agttatttca
      361 aagagatagt ttggagacta cattctgcgg aggggaagct aatgtagcgg tatccttagc
      421 aatatttgga tatcaatcag aatttataac aaagcttccg gattcagatg ttggatatgc
      481 ggcagctaat gctttgcgtt attttggagt aaatgttgat aatattatat ttggagaggg
      541 acgcatggga ctgtattatt tagaaaaagg agcagctcag agaccatcta aagtaattta
      601 tgacagaaaa ttttctgcaa tttcgttagc tgatagtgat gaatttaagt ggaaggagat
      661 cctaaacgat gcaaattggt ttcattggac agggataaat ccagcattgt ctgagaatct
      721 ctttgagacg tccttagatg catgtcaggc tgccaaaaaa agaggtataa caatttcttg
      781 tgatttaaat tatcgatcca agctttggac atcagaggaa gcaaaagaaa agatggaaca
      841 gatactacca ttggttgata tttgtatttg taatgaagaa gatgcagaaa aagtgctagg
      901 aattaaagct gagcatacaa atattaatag tggagaatta aataaagatg gctatgtgca
      961 ggtggctagg gagattcaca agaagtttgg atgtaggtat gtagctacaa ctctgcgtag
     1021 aagttattcg gcaagtagaa atggttggag tgcaatgtta tacgaagcag aaaaagataa
     1081 agcgtatttt tccacagaat acaacataca aattgttgat cgcgttggtg gtggagacag
     1141 cttttctgct ggtataatat atgcgttatg tgaaaatatg tccggacaaa aggctattga
     1201 atttgcgact gcatctagtt gtttaaaaca tactatagaa ggtgatttta atcgaactac
     1261 aattgaggat gtatataatt taatgaatag cggtggaaat ggtcgcgtgt tgcgttagtc
     1321 aacatataaa gataaatcac agtaattttt atggtgatta tctgtcttag tagtgattag
     1381 aataacgtga aaataaattg ttgagcgttt atactgtttt gaaaaataaa tttaacatat
     1441 tgaaaaatac aaatatatac gtatattata aa
//
LOCUS       DANJJH010000209         1463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000209 DANJJH010000000
VERSION     DANJJH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..803
                     /locus_tag="PWS53_002910"
     CDS             <1..803
                     /locus_tag="PWS53_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0873073.1"
                     /translation="YYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRA
                     AFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQ
                     FSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVP
                     KNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKS
                     MDFNSVDEFFIQSAASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(1056..1205)
                     /locus_tag="PWS53_002911"
     CDS             complement(1056..1205)
                     /locus_tag="PWS53_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873074.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          487 a          236 c          251 g          489 t
ORIGIN      
        1 aatattatca acagtataag aaaaacaaat caaactgtgg tagacgtccg cttgttttac
       61 ccgaggaaca atcagaatat attcaaagaa aggttgttca aggatggaca cccgatgtga
      121 ttgttggtcg tgcagcgttt cctattagtt gttctgctcg taccatttat cgtatgttca
      181 aaaaggggct atttgattct tctgacttac cgatgaaagg caagcgtaaa ccgaatggac
      241 atcaagaaag acgtggaaaa caaactttcc gccgctctat tcatgaacgt gaaaaggatt
      301 atagccaatt ctcaaatgag tttggtcacc ttgaaggtga cactatcgta ggtctgaaac
      361 ataaaagtgc tgtaattacc ttagttgaac gattatcaaa agttatcatc acattgaaac
      421 cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa tcaatggttt gaatctgtac
      481 cgaaaaacct attcaaatcc atcacttttg attgtggaaa ggaattttca aattggaaac
      541 agatcagtaa tgtcaatgat attgccattt atttcgctga tccaggaacg ccgtctcaaa
      601 gaggcctaaa cgagaattct aacggattgt tacgtagaga tggtttattg aaatctatgg
      661 atttcaattc agtagatgaa ttttttattc aatctgccgc atctaaacga aataatattc
      721 ctagaaaatc actggattat cgaacacctt tggaagtatt tttgagttac gtaagtattg
      781 atgatctgtc taacttaatt tgacaattaa gattatatta aatattatac agataataac
      841 ggaatattat catcgaaaca gctaacaaaa ttgtcataaa aaacgagatg gttgatatta
      901 gaacttgtgt ttttgtttag gtctagagat aaagagagac aatttccaaa tatatgtaat
      961 gaatacaaaa agacgtttgt cgcggattac cgttacaaac gtcttttatt gattttccga
     1021 gatctcatca gtcgccattt ggaatggttc actccttatc tagattcgtt taatgctcgg
     1081 actgcttgtt cgattgtaat accaaacgca acgttatttt cgttttgtgc atcaatcgcc
     1141 ataaaacggt tagaagttgt atcaaaagct acgcggtatt gcatatttgt ttttgtaaac
     1201 gtcataaaat tcatctcctt tgtaatgtaa tttagttctt gaaagtttcc tttaaacttt
     1261 cgaacttata ccaagtttag catcgtacgt tttttgatgc aattaaaaag catttatcat
     1321 tttccttatt tattttttct taaatggttt agctattttc ttttaatccg ataagtagcc
     1381 caatcatcaa ggttacaaat actattgctt gaatataaat catatgctca cctctttata
     1441 aataacttag caatataaag aaa
//
LOCUS       DANJJH010000210         1454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000210 DANJJH010000000
VERSION     DANJJH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..625)
                     /locus_tag="PWS53_002912"
     CDS             complement(<1..625)
                     /locus_tag="PWS53_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL0873075.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="PWS53_002913"
     CDS             complement(784..1050)
                     /locus_tag="PWS53_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0873076.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="PWS53_002914"
     CDS             complement(1040..1396)
                     /locus_tag="PWS53_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0873077.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DANJJH010000211         1437 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000211 DANJJH010000000
VERSION     DANJJH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..116)
                     /locus_tag="PWS53_002915"
     CDS             complement(<1..116)
                     /locus_tag="PWS53_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873078.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPS"
     gene            391..933
                     /locus_tag="PWS53_002916"
     CDS             391..933
                     /locus_tag="PWS53_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873079.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1089..>1437
                     /locus_tag="PWS53_002917"
     CDS             1089..>1437
                     /locus_tag="PWS53_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL0873080.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          560 a          177 c          276 g          424 t
ORIGIN      
        1 gaaggcgtaa gctcatactc tcgaataatt tctgcgcgag gcttaccagc gagatagaga
       61 tcgacgattt gttgtttgaa ttcttttgaa taggttcttc gttgacgtct tgacatgaaa
      121 aatcctccag tgtgtttttt attattctac acaccttatt ttttctgtct agtctagtgt
      181 agccgattca aattgatgaa gcagatccca aagggctgct tcatcaattt tttcgtaagt
      241 gtccgtacac ttactgtgct tgctcatata ttaatcgatc atatactaat cgtgtaagaa
      301 caactttaag cataatactt atattgttat accatagttt ggaaaaataa aaaattatat
      361 ttcatcaacg tttcataaaa ggtgtgtaga atgttattga aagtagctat aaaatgctat
      421 tgtgtaaaaa ataaaggagg aataatttat gaaaaaatag taccaagagg agcatgccta
      481 ttacttgcat tatgtttgag cttaagcgca gttgggtgtc aaaaaagtaa tgaaaacaat
      541 aaaataaaag aaggacaatc cataagttct gaagaggaag gcatgtcaat taatgcaaat
      601 ggcaaaaatg aaacttttaa gccatcagat tatacattgg aggcaaagaa agaatatgtt
      661 tatgaatatt tagggcttaa atttaaactc tcagacaaat ttagaaatta tatagctgat
      721 aaaaagatag cgatgttaga tgatcaaagt cctatagata aagaactaaa gtatgctatt
      781 ttgacatttg agaaaatgac agaagaacaa aagaatgcag ttatagaaaa gatgggcgat
      841 ggatataaaa attggcaaaa tgagcttgaa agaattggga ctattggtat atttgaaaaa
      901 aatacatcag aagaaaaaaa tctaaaatta taaaatgtga tactcatact aaaataggag
      961 tttcaagtga tggaaaatac gattgctatt taagcacaaa tagcggagct gaaagtaatc
     1021 ttctggatga attaaaaaga actgagattc aaattataga taaaaaagaa cgtcctaaaa
     1081 atggttttgt gctatcagaa aaaactgatt tagaaaatac agaagcattt aacaaagaat
     1141 cggttaaaga tttacgtaaa ctaccaacaa aggacataaa tggcaaggat tttacaagta
     1201 aagattttga gaagtatgat cttactatgg taaatgtatt tgcaacttgg tgtacagctt
     1261 gtgtaaagga aattcctgat ttggttgagg ttcagaatga aatgaaaagc aagggtgtaa
     1321 atatcgttgg agttgttaca gatcctgttg atgataacgg tgaaaacaag gaagcaattg
     1381 aaaagtcaaa gttaatacat gaaaagacaa aggcttcata tcctttctta atgccag
//
LOCUS       DANJJH010000212         1433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000212 DANJJH010000000
VERSION     DANJJH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            105..1406
                     /locus_tag="PWS53_002918"
     CDS             105..1406
                     /locus_tag="PWS53_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0873081.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          409 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atg
//
LOCUS       DANJJH010000213         1422 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000213 DANJJH010000000
VERSION     DANJJH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1350
                     /locus_tag="PWS53_002919"
     CDS             <1..1350
                     /locus_tag="PWS53_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0873082.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          559 a          225 c          307 g          331 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tg
//
LOCUS       DANJJH010000214         1417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000214 DANJJH010000000
VERSION     DANJJH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(150..1337)
                     /locus_tag="PWS53_002920"
     CDS             complement(150..1337)
                     /locus_tag="PWS53_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL0873083.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DANJJH010000215         1403 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000215 DANJJH010000000
VERSION     DANJJH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1282
                     /locus_tag="PWS53_002921"
     CDS             <1..1282
                     /locus_tag="PWS53_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0873084.1"
                     /translation="LSDSNYEVILIDKKSEISYLECGTSLFIKGEVSNLEDLFYQNRM
                     TLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGN
                     NLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVA
                     DRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMV
                     VQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVD
                     FSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARLLSESID
                     YIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQI
                     TLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          562 a          161 c          229 g          451 t
ORIGIN      
        1 cttaagcgat tcaaattatg aagttattct aatcgataaa aaatcagaaa ttagttactt
       61 ggaatgtggg acatcgttat ttataaaagg agaagtaagt aatttagaag atctttttta
      121 tcaaaataga atgacactaa attctgagat aaaaaagtta tttctagaga cagaagttga
      181 caaaattgat attcaaaata aaaagattag ttgtgtaaaa aaagatggta caagattctc
      241 tcaagaatat gacaaagtta tattagctac aggttcaaag caaattatgc tagatatacc
      301 ggggaataat ttagctaata ttttttcgat aaagaatttt ccaaatgcaa aagagatttt
      361 taaagctttg gatgatagta ctattaaaaa agtaggaatt attggagcag gatatattgg
      421 agttgaaatt gctgaagcca taagaaaaag agggaaagaa gtatatcttt tcgatgttgc
      481 agatagagtg ttgtcgactt attacgaccg tagtttttca gataaagttg aagaaatact
      541 atcaaaaaat ggaatacatc tttgtctatc agaaagtcct aaaaaatatt taggaagaaa
      601 aaagattgaa aaaatagtca ctacaagtgg tacccaatac tctatggata tggtcgtaca
      661 agctatagga tttctatcta atagtccaat tggggaaaag gaactcttga gagatgttag
      721 tggagcatat ctaactaatg aataccagca aacaaatata aaagatatat atgctattgg
      781 ggattgtgct acaacttttt ttacgtccat taatagaagt agtgttgatt tttctatttc
      841 taatgctcta agaacagctt atattgcaac tcaacatatt aacaatcaag attttacacc
      901 ttctggttcc caatttacaa atggcttttc tatatttaat tacaattttt acgcagcagg
      961 tttgaattta aaaacagcac gattattaag tgagtctata gattatatag aaatagaaga
     1021 tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga
     1081 aaaaaataca aagagaatag taggtgtaca actttgtagt caagaagatt tatctggact
     1141 attatcaatg ttttctcttg ctatagaaga acaaattaca ttagacaaat tgaaaatgtt
     1201 agattattta tttcatccta gttttagcca accctataat tttattttaa aggcagtaac
     1261 aggattaaac attgaaacat aatcgttact aaatgaaatc aagaaatata ctgtattact
     1321 caattcattg aaaatattta tcataatttt taatagaaaa cactattaaa gagaaaaaag
     1381 agaggtaaat caaatgaaaa aat
//
LOCUS       DANJJH010000216         1394 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000216 DANJJH010000000
VERSION     DANJJH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            185..1354
                     /locus_tag="PWS53_002922"
     CDS             185..1354
                     /locus_tag="PWS53_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0873085.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          513 a          205 c          224 g          452 t
ORIGIN      
        1 ctagcaaaca atattaatga ggtcattaaa tcttattatc tttagtgata taacgaaata
       61 tctacacaga caaaatgata gcgtaaaata ttttgtgtaa atagatattt agggtagaaa
      121 aaaagaaagt ccattctgta aaattaaagt taccacaacc gaaattttat aggaggactt
      181 tctcatgccc aattttacta cagaaattat ggaaacacta atcaataaag gtgatttaga
      241 tgaattattt cgatgtcatt tagaattagc tgttaattca ttattgcagg ctgaattaac
      301 agcttttctt gattatgaaa aatatgatcg tgctgggttc aattcaggta attcccgtaa
      361 tgggaattat tcacgttcat tcagaacaga gtatggagaa ttaaatttgg taattcctag
      421 agatagaaat ggtaagtttt ctcaacaaac tttacctgct tataaaagaa ccaatgactc
      481 tttagaaacc actattattc agctatttca aaaaggaatt actatgtctg aaatctctga
      541 attgattgaa aaaatgtacg ggcatcacta tacgccacaa accatttcaa atatgactaa
      601 aatagtttct gaagatatta ttgcctttaa agaaaggtcc ttagaatcca gatactctgt
      661 catttttatg gatgctactc acattccttt aaaaagacaa actgtttcaa aagaagcagt
      721 ttatattgta attggcattc gattagatgg aaccaaagag gttcttgggt ttagtcttgc
      781 tccaactgaa tctgcttatg tttggaaaga gattcttcaa gacctaaaag accgtggttt
      841 aaaagaggtt ttattagtcg taactgatgg tttaagtggc attaacgata gtattcatag
      901 tgtttatcca aatgctcaat ttcaacaatg ttgtgtccat atatctagaa atatcgctca
      961 taaggttcgt gttagtgatc gacaagaaat ctgtagcgat ttcaaattag tttatcaagc
     1021 aagttcaaaa gaagaagcaa ataatcaaat aaggtttatg atagataaat ggaaaaagca
     1081 gtatccacga gtggttaaat tgctcatgaa tcctgctata ttaacctttt ataatttccc
     1141 tccgtcaatc agaagaacta tttattcaac taatttgatt gaaggtttta acaaacaatt
     1201 aaagaaatat accaaaagaa aagagcaatt tcctaatgaa gaatctctgg agagatttct
     1261 agtatctcaa ttcaacaact ataatcaaaa gtttctatgc cgagttcata aaggttttaa
     1321 agaaatacat gatacattag aatcaatgtt ttaacttgat caacagaatg ggcttttcca
     1381 tttacacaaa atta
//
LOCUS       DANJJH010000217         1394 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000217 DANJJH010000000
VERSION     DANJJH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            53..1392
                     /locus_tag="PWS53_002923"
     CDS             join(53..494,494..1392)
                     /locus_tag="PWS53_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL0873086.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          583 a          182 c          243 g          386 t
ORIGIN      
        1 gatgtgcacc ccaaaagtta gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa
       61 atatacattt gtttttaaga aaaaagtagt ttcagactat ttaaataacg aaggcggcta
      121 caaatatctt gcacataaat atcaaataaa tcgtacactg gttagacatt gggtaaggat
      181 ttataactat catggttggg aaggcttggt tggaggtggc aaaagctaca ctacaaaatt
      241 taaacttgat gttatagaat atatggaaac aaatggtctt tctatccaag aaactgctaa
      301 aaaatttaat atcggttcaa atagaactct aagtaaatgg atagagcaat atgaagaagg
      361 cggtgcttct tcacttgaga gccaaaaaag gggcagaaaa attagtatga attccaagct
      421 aaacattcct aaaaaactta aagatgagtc tcttgaagaa gaagttattc gtttaagagc
      481 agagaacgca tatttaaaaa agttagaaac cttgattcaa gaacaagatt tgagcaagaa
      541 gaaatcaagg ttaaaacaat ccttcagctc aaaaaagaat ttaaattgaa tctattactt
      601 tcaattgcac aattagcgaa gtcaacttat tattactggg taaaaaaatt agataagcca
      661 gataaatata gcaagataaa gcaagaaatt acagcgattg taaaagaatc aagaaactct
      721 tatggttatc gtagagtcac tttagcgtta aagatgaaag gatatacaat caatcataaa
      781 acagttagaa aattaatgtc ccaaatggga cttacctgtc aaatccgaat aaagcgatat
      841 aaatcttata aaggaacagt aggaaaaatt gccaagaatg tgttaaaacg aaatttttca
      901 gtagatacac ccaataaaaa atgggtgaca gacgtcactg aattcaagat aaaaggaaga
      961 aaaatctatc tatctcctat acttgattta tttaacggag aaataattag ttatagtata
     1021 tcaactagtc caacatataa actgattgaa gaaatgcttc aacaagcaat taaaaaaaag
     1081 ggaactgagg gttcactgat tctacattca gatcaaggat ggcagtatca gatgccacaa
     1141 tatcaaaaaa aattaaaaga gaataacatt attcaaagca tgtctagaaa aggaaattgc
     1201 ttagataatt ctgtaataga aaattttttt ggtgtgctta aatcagagtt tttttatcga
     1261 gaaaaattcc gatcaattga gatatttcaa agtaaattaa atgagtatat taggtggtat
     1321 aataacaaaa ggataaaact aaagttaaat ggactatctc cagtagaata tcggaaacag
     1381 tccattaaat agtt
//
LOCUS       DANJJH010000218         1386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000218 DANJJH010000000
VERSION     DANJJH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..117
                     /locus_tag="PWS53_002924"
     CDS             <1..117
                     /locus_tag="PWS53_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0873087.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            172..1386
                     /gene="aph(2'')-I"
                     /locus_tag="PWS53_002925"
                     /pseudo
     CDS             172..1386
                     /gene="aph(2'')-I"
                     /locus_tag="PWS53_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          567 a          126 c          244 g          449 t
ORIGIN      
        1 acgattaaag gcatggaagc catccgagga ttatataaga aaacccgaaa agaaggcact
       61 ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc agcttaaatc
      121 atagataccg taagggattt tattctttat ttaaaacttt gcaacagaac cgaatcatta
      181 aaaaaacatt atacagagcc ttgggaagat gaagttttta gagtaattat tgaatataac
      241 aatgttccta ttggatatgg acaaatatat aaaatgtatg atgagttata tactgattat
      301 cattatccaa aaactgatga gatagtctat ggtatggatc aatttatagg agagccaaat
      361 tattggagta aaggaattgg tacaagatat attaaattga tttttgaatt tttgaaaaaa
      421 gaaagaaatg ctaatgcagt tattttagac cctcataaaa ataatccaag agcaataagg
      481 gcataccaaa aatctggttt tagaattatt gaagatttgc cagaacatga attacacgag
      541 ggcaaaaaag aagattgtta tttaatggaa tatagatatg atgataatgc cacaaatgtt
      601 aaggcaatga aatatttaat tgagcattac tttgataatt tcaaagtaga tagtattgaa
      661 ataatcggta gtggttatga tagtgtggca tatttagtta ataatgaata catttttaaa
      721 acaaaattta gtactaataa gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt
      781 ttaaatacaa atttagaaac taatgtaaaa attcctaata ttgaatattc gtatattagt
      841 gatgaattat ctatactagg ttataaagaa attaaaggaa cttttttaac accagaaatt
      901 tattctacta tgtcagaaga agaacaaaat ttgttaaaac gagatattgc cagtttttta
      961 agacaaatgc acggtttaga ttatacagat attagtgaat gtactattga taataaacaa
     1021 aatgtattag aagagtatat attgttgcgt gaaactattt ataatgattt aactgatata
     1081 gaaaaagatt atatagaaag ttttatggaa agactaaatg caacaacagt ttttgagggt
     1141 aaaaagtgtt tatgccataa tgattttagt tgtaatcatc tattgttaga tggcaataat
     1201 agattaactg gaataattga ttttggagat tctggaatta tagatgaata ttgtgatttt
     1261 atatacttac ttgaagatag tgaagaagaa ataggaacaa attttggaga agatatatta
     1321 agaatgtatg gaaatataga tattgagaaa gcaaaagaat atcaagatat agttgaagaa
     1381 tattat
//
LOCUS       DANJJH010000219         1355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000219 DANJJH010000000
VERSION     DANJJH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..>1355)
                     /locus_tag="PWS53_002926"
     rRNA            complement(<1..>1355)
                     /locus_tag="PWS53_002926"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          281 a          425 c          290 g          359 t
ORIGIN      
        1 acagcccaac ccttgggacc gactacagcc ccaggatgcg acgagccgac atcgaggtgc
       61 caaacctccc cgtcgatgtg gactcttggg ggagataagc ctgttatccc cagggtagct
      121 tttatccgtt gagcgatggc ccttccatgc ggaaccaccg gatcactaag cccgactttc
      181 gtccctgctc gacttgttgg tctcgcagtc aagctccctt ctgcctttac actcttcgaa
      241 tgatttccaa ccattctgag ggaacctttg ggcgcctccg ttacctttta ggaggcgacc
      301 gccccagtca aactgcccat ctgacactgt ctcccaccac gattagtggt gcgggttaga
      361 gtggccataa cgcaggggta gtatcccacc agcgcctcca tcgaaactag cgttccgatt
      421 tctacggctc ctacctatcc tgtacatgcg gtacagacac tcaatatcaa actacagtaa
      481 agctccatgg ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag gtactaaaat
      541 ttcaccgagt ctctcgttga gacagtgccc aaatcgttac gcctttcgtg cgggtcggaa
      601 cttacccgac aaggaatttc gctaccttag gaccgttata gttacggccg ccgtttactg
      661 gggcttcaat tcgtaccttc gcttgcgcta agcactcctc ttaaccttcc agcaccgggc
      721 aggcgtcagc ccctatactt catcttacga ttttgcagag acctgtgttt ttgataaaca
      781 gtcgcttggg cctattcact gcggctgatc gtatgatcag caccccttct cccgaagtta
      841 cggggtcatt ttgccgagtt ccttaacgag agttctctcg ctcaccttag gattctctcc
      901 tcgactacct gtgtcggttt gcggtacggg ccgctgtttt ctcactagaa gcttttctcg
      961 gcagtgtgac atcaggaact tcggtactat tatttccctc cccatcacag cttgtcctta
     1021 agaaaagaag catttgactc ctcttcagac ttactgcttg gacagacatt tccgatcgtc
     1081 tgcattcctt agcctcctgc gtccctccat tgctcaaaca aaaacaacgg gtacaggaat
     1141 atcaacctgt tatccatcgc ctacgcctgt cggcctcggc ttaggtcccg actaaccctg
     1201 ggcggacgag ccttccccag gaaaccttag tcattcggtg gacaggattc tcacctgtct
     1261 ttcgctactc ataccggcat tctcacttct aagcgctcca gcagtcctca cgatctgcct
     1321 tcaacgccct tagaacgctc tcctaccaat acacc
//
LOCUS       DANJJH010000220         1310 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000220 DANJJH010000000
VERSION     DANJJH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(146..445)
                     /locus_tag="PWS53_002927"
     CDS             complement(146..445)
                     /locus_tag="PWS53_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0873088.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            796..>1310
                     /locus_tag="PWS53_002928"
     CDS             796..>1310
                     /locus_tag="PWS53_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0873089.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNID"
BASE COUNT          443 a          209 c          181 g          477 t
ORIGIN      
        1 taattaaagg ataaaataca atataaagaa acatctgttt ctttatttag aataaaaaaa
       61 tacagactct gttcaaataa atgaacctag cctgcattat gagattgact atacttttta
      121 attcattgag atgaataact cgtttttata aatgcccaaa agtaatcggt gccgaagcca
      181 ttcctcgacc taattctttt tcaaattttc ctattgttga tatctccata taaaacggtg
      241 atactggttt agacaattct cccctgattg ctaacggacg taaactacgt tccaaattat
      301 ttatgacttt aggataatac tgaccttttt ctaagcctgt ctttgcgtct atcaaaagta
      361 tacgttcctt ttctttgatc tctggattta aaacgagatc atataatttt agtaccatgt
      421 tttctatttc ttcactttta gacatcacga tttcctccca aaaatatatt tcttcccact
      481 acattctata aacaaagtat acgctacatc tagatttttt taaatcatta ctggattgtt
      541 aaaacctttc aatacactgt tatggcaacg atttttattt taaaaacatt ctaaattaaa
      601 tcagtaaaaa tttttattct aagattttta ttagaattac gcctaatttg tgtctctcag
      661 ggcaattcta agaacgtttc aagagatagc gacactatcg ttatcgaaaa aggcgaaaac
      721 ctatctcatt ttctgctaat gtaagttggt ttagcttttg ttaatatgac tttatgaaaa
      781 tggaggtaat taaatatgat ttttcctaaa gttaatgaag caagcttgaa taaaggatcc
      841 ttttttcata tctatgaaaa cgaagatttt gaactgtata atatcctttc acaaaaagag
      901 gtcatcgaaa accaaataat ctgccatcca attatttaca aatctatttt caagtatgca
      961 ttttttgctt ccaatgattt tcaaagaatg gagattaccg actgtatatt cgaaaactgt
     1021 gatttttcag gctgtatatt gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt
     1081 aaattaacag gtactaaatt gatggaaagt tatattggga acacgttatt cgagaattgt
     1141 aaaatggact atgtaaattt atctggttca aacataaaag aaagtacctt tgagcactct
     1201 gtcttatcaa gcgcagattt tgtagactgt tccctcacta aaataatgtt ttctactaat
     1261 gatttagaaa atgctagctt ttttgatact gatttaaaaa atatcgattt
//
LOCUS       DANJJH010000221         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000221 DANJJH010000000
VERSION     DANJJH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1244
                     /locus_tag="PWS53_002929"
     CDS             <1..1244
                     /locus_tag="PWS53_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL0873090.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANJJH010000222         1293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000222 DANJJH010000000
VERSION     DANJJH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>1293
                     /locus_tag="PWS53_002930"
     CDS             <1..>1293
                     /locus_tag="PWS53_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HDL0873091.1"
                     /translation="SQQLLDFLLNYRFSENVGNLKNIIQLTCANAFYFLDDLEELYIQ
                     MYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEYLYQTWKKDTDKQQ
                     LFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQIEFDYYYLQITSIF
                     LNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYLTKIQKNVLVILFK
                     LMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKIN
                     RYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDL
                     PLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPRDTDLV
                     IETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDV"
BASE COUNT          477 a          164 c          221 g          431 t
ORIGIN      
        1 tatctcaaca attattagat tttttattaa actatcgctt tagtgaaaat gttggcaatt
       61 tgaaaaatat cattcaattg acctgtgcca atgcctttta tttcttagat gatttggaag
      121 aactttatat tcagatgtat catttaccac aatgggtgaa tttcaacggc atgaaggtta
      181 tgaaaagaca gaatgaatta aaatatattc aaatggatga tttggaaaaa gtagacgaag
      241 tttttaatat caacgaactt atagagtatc tttatcaaac gtggaagaaa gacactgata
      301 agcagcagct ttttgagaaa tatgatagat attttagaca aaagaatgtt gatatccaaa
      361 ataattcagc tttaacatat cccacctatc aagagattcg atttagacag tatgttaagg
      421 aaatagagag agattttcaa atagaatttg actattatta tcttcaaata acgtctatct
      481 ttttgaattc ttggatttat caaatccaat taacatcgga cgataaatta tattccttca
      541 tcaatgatat taatagttgg tgtagtcgag aagttcaatt agtcaatcgt tttattcaaa
      601 taggggatat ttatttaaca aaaattcaaa aaaatgtttt ggttatttta tttaagttaa
      661 tgtgtagtaa agaatttcag caaaaacgtt tagcactgat tttggctcat ggagatctaa
      721 cggcaacctg tatagcaagt actgctaata ggatcgttga gagatatata tacgacgcca
      781 ttgatatgcc actaatgact tcaagtagtg agattgcaga caaaattaat agatacttac
      841 aagagtgtac cggatatgaa gaaattattt tacttgttga tatgggttca ctggagaaaa
      901 tttatctaaa tttggaacta gacgctcaaa aaaaattcgg actactaaac aatgtttcaa
      961 caagaatggc tattgatgtt gcaaatcaga tattattaga tttaccgctt aaacagattc
     1021 ttgaacaagt aaaagaaaat caaaatgtgg attatattta tgaatcatca aatattaaga
     1081 aggatatgat tgtctgttct tgtgcaagcg gattaggaac ttcgtttaaa ttaaaaaaga
     1141 tacttgaaga tagctttcca agggatactg atcttgtcat agaaacgtgt gactattact
     1201 ctttagtgaa tgaaaactat ttatcggagc ttcgtaaaga gaacaatatt ctttttattg
     1261 ttggaacact aaatccacat ctagaagatg tag
//
LOCUS       DANJJH010000223         1287 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000223 DANJJH010000000
VERSION     DANJJH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..848
                     /locus_tag="PWS53_002931"
     CDS             <1..848
                     /locus_tag="PWS53_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0873092.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DANJJH010000224         1259 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000224 DANJJH010000000
VERSION     DANJJH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            14..1093
                     /locus_tag="PWS53_002932"
     CDS             14..1093
                     /locus_tag="PWS53_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0873093.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          373 a          300 c          309 g          277 t
ORIGIN      
        1 aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg gaaaacatct
       61 ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt ccaaccgtca
      121 ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg atcgcggaac
      181 catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct actattctgt
      241 ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact gtggcagacg
      301 ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa tgttaaatga
      361 aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt tcgattgtgc
      421 catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca tgcgaacaaa
      481 gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc caggacaccg
      541 tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag ttgaaacacg
      601 ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca agtcggatgc
      661 cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa agttgaacgg
      721 gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg ctggcgctaa
      781 cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt ctgggctaca
      841 cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt cttgggagcg
      901 tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg gaagcccgat
      961 tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg attacccgcg
     1021 gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca agcaagaacg
     1081 gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat ggcttcatgt
     1141 ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag ttcattatca
     1201 aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga gttacctgt
//
LOCUS       DANJJH010000225         1254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000225 DANJJH010000000
VERSION     DANJJH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            470..1105
                     /locus_tag="PWS53_002933"
                     /pseudo
     CDS             470..1105
                     /locus_tag="PWS53_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1120..>1254)
                     /locus_tag="PWS53_002934"
     CDS             complement(1120..>1254)
                     /locus_tag="PWS53_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0873094.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          429 a          208 c          220 g          397 t
ORIGIN      
        1 atatagtatc atttattttt ttaaaacact agattactca ctatataaaa tgtgattaat
       61 tttattatta ataaaatatt ttttatatta caaaagtagt gtttcaaagt tagtagagaa
      121 aatgtagtta tatatttaaa tttttaccag aaagaaatga acaccttcaa ttatctaaat
      181 ctcgaaaatg gttagatgta atcatcagga aaaacaatct taaacgaatt actattcatg
      241 gtcttagaca cacacatgct agcttgcttt tagcaagcta gtgcaaatat taaagatgtg
      301 caagagcgtt taggccactc ttctattcaa atcattatgg atctatatat tcatattaca
      361 gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat ataggctcta taatttatgg
      421 gactgatgaa tcaaaattga ttcttcagtc ccattgttat tttaggtata tgattcgtac
      481 attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt
      541 gatggtggat gaatttcgtt cgcacgcttc aattgtggat aaaatgagct ttatttgtgc
      601 ggatggcgag actggaaaac tcattgatat tttgcctaca cgaaaattgt ctcgattaac
      661 acactatttt agtcgctgtg caaatcctga agcggtggaa tatttagtga cagatatgaa
      721 cgccgcctac ttccaactga caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg
      781 ttttcatatc atcaaacatt tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa
      841 aagattacac cagttagggc aacgacggga agccgagaaa gtgaaaaaga actggcgctt
      901 tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac aaaacatggc gcagttttcg
      961 tgcacctaaa tacccgctac tcactgaagc aatgatgatt gatcgattgc tggcattttc
     1021 aacgccttta aaggacgcct atactttttt ccatgagctg accgaagcct ttagagacaa
     1081 ggaatcagac ttatttttta ccttatatta tttgtcaaat taagcgagac aaaactttcc
     1141 atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa tttagtgatt ttctagggat
     1201 atggtttctt ttggacgcaa cggatgagat gaatccttga ttgacttggt tgaa
//
LOCUS       DANJJH010000226         1238 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000226 DANJJH010000000
VERSION     DANJJH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            39..656
                     /locus_tag="PWS53_002935"
     CDS             39..656
                     /locus_tag="PWS53_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873095.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          456 a          167 c          196 g          419 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacattta
//
LOCUS       DANJJH010000227         1183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000227 DANJJH010000000
VERSION     DANJJH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..153
                     /locus_tag="PWS53_002936"
     CDS             <1..153
                     /locus_tag="PWS53_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0873096.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="PWS53_002937"
     CDS             159..575
                     /locus_tag="PWS53_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873097.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="PWS53_002938"
     CDS             681..944
                     /locus_tag="PWS53_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0873098.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="PWS53_002939"
     CDS             944..>1183
                     /locus_tag="PWS53_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0873099.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DANJJH010000228         1144 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000228 DANJJH010000000
VERSION     DANJJH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWS53_002940"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="PWS53_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL0873100.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DANJJH010000229         1114 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000229 DANJJH010000000
VERSION     DANJJH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..897)
                     /gene="vanY"
                     /locus_tag="PWS53_002941"
     CDS             complement(<1..897)
                     /gene="vanY"
                     /locus_tag="PWS53_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL0873101.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          399 t
ORIGIN      
        1 agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt ttcctgatat
       61 ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag gataataaaa
      121 gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt ttaggtaatc
      181 catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg gtaaaccaac
      241 atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt cctctggata
      301 acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt caggggctcg
      361 ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat gctcactata
      421 acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt gctcatcaaa
      481 gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag catcattgac
      541 catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat caagcaaccc
      601 gtatccattt attaattcgt catgtttaga taaattcacg atatctgact tcacactttc
      661 ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa cctgttcttc
      721 tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat gttctttggg
      781 attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt cattaacgta
      841 gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact tcttcatttt
      901 cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc ttccccttaa
      961 acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt caattatttc
     1021 gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg atacccatgc
     1081 tgaatttaac gaggctgaac tgcctgttga caat
//
LOCUS       DANJJH010000230         1109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000230 DANJJH010000000
VERSION     DANJJH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1087
                     /locus_tag="PWS53_002942"
     CDS             <1..1087
                     /locus_tag="PWS53_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0873102.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          194 c          215 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccg
//
LOCUS       DANJJH010000231         1090 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000231 DANJJH010000000
VERSION     DANJJH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            86..778
                     /locus_tag="PWS53_002943"
     CDS             86..778
                     /locus_tag="PWS53_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0873103.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          483 a          122 c          164 g          321 t
ORIGIN      
        1 aacagaaagt gtctaaagaa agcttgacga agaaaataat ttttaataac ttatttaagg
       61 aaattttttg aaggagaaga taaaagtgag aaaaaagatt attattaatg ctggagtaat
      121 tataggattg atgttagtaa ttttctccgg aaagcaatat gctgataata ttttattatg
      181 gggaggggaa gataatattg aaaagatcaa caacaattta actatattag acacagctct
      241 agaaaataaa gagcaaaaaa ttagtatttt aaattcacaa ttgtcttcaa acaaccaaac
      301 tcttgaacaa ctaagaacta atattgaagt ttccaaacaa aaagtggcat ctttagaaaa
      361 cgacaagaat caattagtat cagagaaaac taatttagaa aatcaattgt cagcaaaaga
      421 ctctgaattg caaggaaaac aaaatgaaat taatgcgaaa atagacgaaa ttaaccaaaa
      481 agtagctgaa ataaatcgat taaatagcga ttggaataat cgtttacaaa atgaaaagaa
      541 caaatttaat gaagaacaat ctactattaa tagtttaaac cagcaaatta atcaaatgtt
      601 gagtgaaaaa aataacattc aaaatcaact gcaagctact cagcaaaagc tagaagaaga
      661 aaagtcaaaa aatagtagtt tgaataatta cattgaaaag cttcaaaaag ctaaaaatga
      721 tgtagaggat accgcaaata aagcacaaca aattgtaaat gaacatacaa tccaatagat
      781 atagttttaa tttaacaaat ggtatgtttg gagataatga gatagaatat aaatgtcata
      841 atattataca atctaaacac cgtttccttt tctatttttg atgacatcag tatatagcta
      901 ctcgaaagag tagtcttttt attattctcg cttattattg atatgaaaat ttcgatttga
      961 caaaaggtac cgaataattt tgtaatatga attattatca agaagtaatt atgatgataa
     1021 catatggaaa ctagttagta ataataacag gtgattaaac ttttgaacga cttgtaattc
     1081 tatatttttt
//
LOCUS       DANJJH010000232         1074 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000232 DANJJH010000000
VERSION     DANJJH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..276)
                     /locus_tag="PWS53_002944"
     CDS             complement(<1..276)
                     /locus_tag="PWS53_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873104.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
     gene            838..>1074
                     /locus_tag="PWS53_002945"
     rRNA            838..>1074
                     /locus_tag="PWS53_002945"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          351 a          194 c          205 g          324 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagac ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaataaac atttttaaca
      781 agcaagcaat atgctagcaa acaaattgag cttaacaatc gcaagattgt tcgaactttt
      841 tatgagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc
      901 gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg cgaacgggtg
      961 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata
     1021 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg tgtc
//
LOCUS       DANJJH010000233         1061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000233 DANJJH010000000
VERSION     DANJJH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(36..992)
                     /locus_tag="PWS53_002946"
     CDS             complement(36..992)
                     /locus_tag="PWS53_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873105.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DANJJH010000234         1051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000234 DANJJH010000000
VERSION     DANJJH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="PWS53_002947"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="PWS53_002947"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="PWS53_002948"
     tRNA            203..276
                     /locus_tag="PWS53_002948"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="PWS53_002949"
     tRNA            316..405
                     /locus_tag="PWS53_002949"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="PWS53_002950"
     tRNA            420..491
                     /locus_tag="PWS53_002950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="PWS53_002951"
     tRNA            507..579
                     /locus_tag="PWS53_002951"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="PWS53_002952"
     tRNA            589..661
                     /locus_tag="PWS53_002952"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="PWS53_002953"
     tRNA            697..772
                     /locus_tag="PWS53_002953"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="PWS53_002954"
     tRNA            774..854
                     /locus_tag="PWS53_002954"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="PWS53_002955"
     tRNA            861..934
                     /locus_tag="PWS53_002955"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
     gene            937..1009
                     /locus_tag="PWS53_002956"
     tRNA            937..1009
                     /locus_tag="PWS53_002956"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:970..972,aa:His,seq:gtg)
BASE COUNT          250 a          234 c          285 g          282 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca tcattcgccc tttttattct
     1021 tggggtatag ccaagcggta aggcaaggga c
//
LOCUS       DANJJH010000235         1051 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000235 DANJJH010000000
VERSION     DANJJH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>1051
                     /locus_tag="PWS53_002957"
     CDS             <1..>1051
                     /locus_tag="PWS53_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL0873106.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLT"
BASE COUNT          335 a          211 c          207 g          298 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga c
//
LOCUS       DANJJH010000236         1041 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000236 DANJJH010000000
VERSION     DANJJH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..970
                     /gene="bsh"
                     /locus_tag="PWS53_002958"
     CDS             <1..970
                     /gene="bsh"
                     /locus_tag="PWS53_002958"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL0873107.1"
                     /translation="TSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLD
                     THYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPW
                     ILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYD
                     NPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSS
                     VSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCN
                     VDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          373 a          131 c          180 g          357 t
ORIGIN      
        1 tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg attatgaaat
       61 atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt ttcgaaaggt
      121 aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag ctgactaccc
      181 tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa atttttctgg
      241 gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt ttgaatttat
      301 tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt taaaaaatat
      361 caatttagca aatataaatt atagtgacga acttccttta tcccctttac attggctatt
      421 agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc atatatatga
      481 taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat ttaatttaaa
      541 caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc aaataagttt
      601 gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat cctcagtatc
      661 tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt cagagtcaga
      721 aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag gtttgtgtga
      781 tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg ttgacaaagg
      841 aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata tgaataaaga
      901 agacttagat agtcataagt taattagtta tccaattata gaaaaacaac aaattaaata
      961 tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac aaaaaggcaa
     1021 agatattttc aaaacatttt t
//
LOCUS       DANJJH010000237          999 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000237 DANJJH010000000
VERSION     DANJJH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>999
                     /locus_tag="PWS53_002959"
     CDS             <1..>999
                     /locus_tag="PWS53_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDL0873108.1"
                     /translation="THWTAQSYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVS
                     LTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVL
                     PTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQ
                     AFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFL
                     TEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQ
                     NLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          334 a          197 c          184 g          284 t
ORIGIN      
        1 gaacccattg gacggctcaa agttattttg ttcaacccag acattcaatc gcgaatcatg
       61 tcagatataa aattgcttct ttactgactg aaaaagtatc tttatctttt attgcgaaaa
      121 attgtcaggt atctttgacc accgttattc gtacgttgaa agagtttaaa agctacttac
      181 caaagcaatc taagaggatt ctccccagag tattgatggt tgatgaattt cgttcgcacg
      241 cttccataga agataaaatg agctttattt gcgcagatgg cgaaacagga caattaattg
      301 atgtcttgcc tacgcgtaaa ttgcctcgat taacaagcta tttcttaggc tgcgctaatc
      361 ctgaagaagt aaaattttta gtcacagaca tgaacgccgc ctacttccag cttaccaaac
      421 gtattctgcc aaatgcgaaa gtagtgattg atcggtttca tattatcaaa cacatgaatc
      481 aagcctttaa tgagttgcgt atccgtgaaa tgaatgaact tcgtaaagta ggacagaaaa
      541 gtcaggcaga aaaactgaaa aagaactggc gctttttgct aaaaaatcgt gcaaacatca
      601 accattatga atacaaaaca tggaaaagtt ttcgagcacc aaaataccca tttcttacgg
      661 aagcgatgat gattgatcga ttgcttggct tttctacgtc cctgaaggag gcgtatccct
      721 attttcatga attagttgaa gcctttcgag acaaagaccc tgacttattt ttctccttat
      781 tggcagaact tcccgaaacg ttagatgacg gctttcggga aaagctccaa aaccttctga
      841 cctatgaaga aggcatcacc aacgcgatga tctatcctta caccaatgga aaaatagaag
      901 cgaagaatac ccatataaag acaatgaaac gagtatccta cggatttaaa tcatttgaga
      961 acatgagaat tagaatcttt ttgatcaatc aattaatca
//
LOCUS       DANJJH010000238          997 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000238 DANJJH010000000
VERSION     DANJJH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(26..913)
                     /locus_tag="PWS53_002960"
     CDS             complement(26..913)
                     /locus_tag="PWS53_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0873109.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DANJJH010000239          959 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000239 DANJJH010000000
VERSION     DANJJH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(107..511)
                     /locus_tag="PWS53_002961"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="PWS53_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="PWS53_002962"
     CDS             complement(570..>959)
                     /locus_tag="PWS53_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873110.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DANJJH010000240          953 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000240 DANJJH010000000
VERSION     DANJJH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..671
                     /locus_tag="PWS53_002963"
     CDS             <1..671
                     /locus_tag="PWS53_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873111.1"
                     /translation="TSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIP
                     KIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKK
                     ETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEK
                     TINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVE
                     YGII"
     gene            658..>953
                     /locus_tag="PWS53_002964"
     CDS             658..>953
                     /locus_tag="PWS53_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0873112.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIV"
BASE COUNT          350 a          127 c          137 g          339 t
ORIGIN      
        1 caacaagtaa aaacgttcaa agagttttta aaatattaaa taaaaatagc atcttcaaaa
       61 aaaaaattga cgattatttt gtctattgtc cggaattgtt actttatgat gtaaaaaata
      121 taaaaaaaat tcccaaaata gatgaaaaca gtaaattgat gaaaaacata caatcaatct
      181 ttgggataga agaagttttt gtcggagata aatgtatctt ttttgatcaa cctttattat
      241 ctgatggtta tggcatagat gaaacagata ttttcgctaa agtaaatagt atattttctg
      301 gaaaaaaaga aactttaatt aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa
      361 aaaacatatt gaattccagt ttaccgtttg aagtcttgct acttgaaaat gacttctctt
      421 caacactttt gattagtcct atatctacaa tttctttttc accatctatg atgtttgaaa
      481 agacgatcaa ctctattatg ttggctaaat taattttaaa tgaatataca gcattatctg
      541 aagataaaaa agaaacattg acaaatattg ttcttttctg tgaacattat aaccgtctat
      601 cagaagaaaa aattttactc ccagaaaact gggcagaatt aagaaagttg gtagagtatg
      661 ggattattta acacactttc caagtcaaaa ttagttaagt ccagtttctg gtatactata
      721 gggaattttt ttattaaggg aatttcattc ataactattc ctatttttgt aaatattact
      781 actcaagcag aatatggttt gattaataat ttcacttcat ttgtatcaat tttttcgatt
      841 cttgttgggc taagcctaaa ttcggcaatt aataatgcaa gttttgacta taaagatgac
      901 ataaatgaat ttatgtcatc ggtactttct ctagctacac tttcgttaat tgt
//
LOCUS       DANJJH010000241          949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000241 DANJJH010000000
VERSION     DANJJH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            10..420
                     /gene="spx"
                     /locus_tag="PWS53_002965"
     CDS             10..420
                     /gene="spx"
                     /locus_tag="PWS53_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0873113.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            434..790
                     /locus_tag="PWS53_002966"
     CDS             434..790
                     /locus_tag="PWS53_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL0873114.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          320 a          153 c          188 g          288 t
ORIGIN      
        1 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
       61 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
      121 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
      181 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
      241 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
      301 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
      361 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
      421 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
      481 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
      541 cgatctaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
      601 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
      661 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
      721 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
      781 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
      841 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
      901 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatat
//
LOCUS       DANJJH010000242          948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000242 DANJJH010000000
VERSION     DANJJH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            659..>948
                     /locus_tag="PWS53_002967"
     rRNA            659..>948
                     /locus_tag="PWS53_002967"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          363 a          161 c          191 g          233 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtacctaat acatgcaagt
      721 cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aagaggagtg gcgaacgggt
      781 gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac aggtgctaat
      841 accgtataac aatcaaaacc gcatggtttt gatttgaaag gcgctttcgg gtgtcgctga
      901 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcacc
//
LOCUS       DANJJH010000243          941 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000243 DANJJH010000000
VERSION     DANJJH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..>941)
                     /locus_tag="PWS53_002968"
     rRNA            complement(<1..>941)
                     /locus_tag="PWS53_002968"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          199 a          292 c          211 g          239 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca g
//
LOCUS       DANJJH010000244          940 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000244 DANJJH010000000
VERSION     DANJJH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            111..>940
                     /locus_tag="PWS53_002969"
     CDS             111..>940
                     /locus_tag="PWS53_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL0873115.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          331 a          136 c          173 g          300 t
ORIGIN      
        1 caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa ggaagtccct
       61 tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat atgaacgatt
      121 ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat ttgtttcgtc
      181 gtcacctaga attggctatt aacgctttat tacaagctga attaacggta tttcttgact
      241 atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg aattattcgc
      301 gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat aggaatggag
      361 acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta gaaaccacta
      421 ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta atcgaaaaaa
      481 tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt gtgtctgaag
      541 atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt tttatggatg
      601 ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat atagtaattg
      661 gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca actgaatctg
      721 cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa gaggttttgt
      781 tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt tatccaaatg
      841 ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag gttcgtgtta
      901 gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DANJJH010000245          931 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000245 DANJJH010000000
VERSION     DANJJH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>931
                     /locus_tag="PWS53_002970"
     CDS             <1..>931
                     /locus_tag="PWS53_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0873116.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DANJJH010000246          930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000246 DANJJH010000000
VERSION     DANJJH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(193..591)
                     /locus_tag="PWS53_002971"
     CDS             complement(193..591)
                     /locus_tag="PWS53_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0873117.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(588..824)
                     /locus_tag="PWS53_002972"
     CDS             complement(588..824)
                     /locus_tag="PWS53_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0873118.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          289 a          166 c          126 g          349 t
ORIGIN      
        1 cagcccacta gttctaaacc actatttcct actattcact cttttgtatt ccttttaaat
       61 caattatttc aaaattaatc catattttcc atatttttat gaaaaaacta ttttgtatga
      121 ttaaacataa aaaaatcagc atattcctta atgacatgct gatctaattg agaaataaag
      181 tttttaattc gttcatttta ttgcggtttg tcgtaaataa ctggttatct tggactttag
      241 tgaatcgaaa tccaatgaac tagtggtaat cattatgata aaatcaacag cttcttgacg
      301 agaaaaagca actggacaat tattcaattg aagaaacaac aacatggcaa caagagcagt
      361 tcgtttattg ccattatgaa aaggatgttt tttagctaaa ttaattgcta aaatcgccgc
      421 tttttcatag atttcaggat agagttcttg attgaatact gcttgtgaag gctgtttgac
      481 ggccatatct aatgcatttg catcttttac accgatcatt tcgcctggcg agtgacgtaa
      541 aataactttc gcgttgattt tgataatttc tttagtcgaa agatacctca taaatcaacc
      601 aaatctttta acgcgtcttc atattgattc atgatcgaat caactaatga atcaatatca
      661 ggttcttctt gtgctttgat cattcgtaca gaaccatcag attgaaaaac ataactgatt
      721 gcgtcacctg tctgcacacc catgaattct tttacttctg gtgggatagt tgttacatcg
      781 ctattgccag attttctaat tttatacttt ttcatttttt tcatatttcg ccaccctcta
      841 taccattctc attctatagt aagtacatta tatccaagta tatactttta atcaatgtat
      901 atacttttat tttgcttttt cttacgaaaa
//
LOCUS       DANJJH010000247          904 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000247 DANJJH010000000
VERSION     DANJJH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            265..888
                     /locus_tag="PWS53_002973"
     CDS             265..888
                     /locus_tag="PWS53_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0873119.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DANJJH010000248          877 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000248 DANJJH010000000
VERSION     DANJJH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            86..355
                     /locus_tag="PWS53_002974"
     CDS             86..355
                     /locus_tag="PWS53_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0873120.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="PWS53_002975"
     CDS             348..605
                     /locus_tag="PWS53_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0873121.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          334 a           97 c          168 g          278 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgctta
//
LOCUS       DANJJH010000249          853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000249 DANJJH010000000
VERSION     DANJJH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            147..533
                     /locus_tag="PWS53_002976"
     CDS             147..533
                     /locus_tag="PWS53_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0873122.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            627..818
                     /locus_tag="PWS53_002977"
     CDS             627..818
                     /locus_tag="PWS53_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873123.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          314 a          132 c          139 g          268 t
ORIGIN      
        1 aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt
       61 attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt
      121 aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt
      181 tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag
      241 aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata
      301 ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa
      361 aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat
      421 ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca agacagggtg
      481 cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt taatatctgc
      541 ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag aaatagatta
      601 taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc attttagcaa
      661 aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagctct ttgattcata
      721 agcaatcctg gagaactgga aaatatgatc aagattgtta tcaagaatgt atgcttgcaa
      781 tatatctagc tatctcgaaa ttcgaaatta aagaataact caactaaggg agtctgtgac
      841 ataagtaaaa aga
//
LOCUS       DANJJH010000250          839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000250 DANJJH010000000
VERSION     DANJJH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            81..782
                     /locus_tag="PWS53_002978"
                     /pseudo
     CDS             81..782
                     /locus_tag="PWS53_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          275 a          139 c          163 g          262 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacaat tattccgtta tatttatgga tgcgacgcat atccctgtga
      121 agcgacagac tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa
      181 ccaaagaagt tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag
      241 ttcttcaaga tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat
      301 taagctgtat tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt
      361 gtgtgcatgt atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt
      421 gtgaggattt caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg
      481 actttatgac taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc
      541 ctgccctatt aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga
      601 acctgattga aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc
      661 ctaatgaaga atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat
      721 ttttaggtag aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt
      781 aactgtaatt aacagaatgg attttccatt tacatataat tcttgacgct accataatc
//
LOCUS       DANJJH010000251          816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000251 DANJJH010000000
VERSION     DANJJH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            136..309
                     /locus_tag="PWS53_002979"
     CDS             136..309
                     /locus_tag="PWS53_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873124.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          308 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaa
//
LOCUS       DANJJH010000252          811 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000252 DANJJH010000000
VERSION     DANJJH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..672
                     /locus_tag="PWS53_002980"
     CDS             <1..672
                     /locus_tag="PWS53_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873125.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DANJJH010000253          768 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000253 DANJJH010000000
VERSION     DANJJH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(109..>768)
                     /locus_tag="PWS53_002981"
     CDS             complement(109..>768)
                     /locus_tag="PWS53_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDL0873126.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          176 c          147 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcg
//
LOCUS       DANJJH010000254          752 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000254 DANJJH010000000
VERSION     DANJJH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..348)
                     /locus_tag="PWS53_002982"
     CDS             complement(<1..348)
                     /locus_tag="PWS53_002982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873127.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKK"
BASE COUNT          234 a          134 c          109 g          275 t
ORIGIN      
        1 ttttttgatt ttatcatctg cacaaaggag attttttaaa tctaatcctt cttttatttc
       61 aaaagttgaa ttttttaaat ctataagagt aaaaccaatt ccttcatact gaatttctga
      121 aaatcgaatt tttctcccag ttgatagatc aaggggcaga aaacctttta ttacattgtg
      181 agtgtcacca tcatttacaa tgtcattagg aattttgatt ttagaattct gtggagcacc
      241 tattggaaga taataattca ttataatttt gtagtcttca tgaggaaata aagtactttt
      301 aggattgcta agatattctc gtaccgaatc taagaatggc ttatccattg aatcttcatc
      361 cccatttaaa ctaaaaaaca ttcctacaat tgcttttgca actctaagtg aattcatttt
      421 ctttattgaa tgtgttaaat cagacttacc attctttaaa cttgtattgt tataaaggaa
      481 atttttattg tcttctgcaa atctaatata ttcctcaaca tactcactac caaaaaaact
      541 attacattta ctacataacg tattggaatg gcaacagttt ttttgacaaa ttttataagg
      601 tgcagaactt ctttccgtat gctattccga ttgtccttga caatgagcct cctcggatgt
      661 gacgatcttc ggcaggagct aacagttaaa gttagactgc ctcgctagcg ttagcacatc
      721 cgagctcatt atcaaggatt cgctgcgcca at
//
LOCUS       DANJJH010000255          742 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000255 DANJJH010000000
VERSION     DANJJH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            178..381
                     /locus_tag="PWS53_002983"
     CDS             178..381
                     /locus_tag="PWS53_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDL0873128.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="PWS53_002984"
     CDS             381..731
                     /locus_tag="PWS53_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0873129.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DANJJH010000256          733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000256 DANJJH010000000
VERSION     DANJJH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.269"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>733
                     /locus_tag="PWS53_002985"
     rRNA            <1..>733
                     /locus_tag="PWS53_002985"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DANJJH010000257          712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000257 DANJJH010000000
VERSION     DANJJH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..446)
                     /locus_tag="PWS53_002986"
     CDS             complement(<1..446)
                     /locus_tag="PWS53_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873130.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DANJJH010000258          683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000258 DANJJH010000000
VERSION     DANJJH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            96..293
                     /locus_tag="PWS53_002987"
     CDS             96..293
                     /locus_tag="PWS53_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL0873131.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            295..606
                     /locus_tag="PWS53_002988"
     CDS             295..606
                     /locus_tag="PWS53_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0873132.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
BASE COUNT          268 a           63 c          121 g          231 t
ORIGIN      
        1 attattttgc tacgtttcaa gaaggaattg ttttttgaaa tttaatgaat gttagaataa
       61 tagtctaaaa acattaattt aggggtgatt agattatgaa acatttaaaa attttgtcta
      121 ttaaagagac acaacttatc tatgggggta ccactcatag tggaaaatat tatggaaatg
      181 gagtgtattg cactaaaaat aaatgtacgg tcgattgggc caaggcaact acttgtattg
      241 caggaatgtc tataggtggt tttttaggtg gagcaattcc agggaagtgc taaaatgaaa
      301 aaaaatgcta agcaaattgt tcatgaatta tataatgata tatctataag taaagatcct
      361 aaatattctg atattcttga ggttttacaa aaggtatatt taaaattaga aaaacaaaaa
      421 tatgaattag atcccggtcc tttaataaat agattggtga attatctata ttttactgct
      481 tatactaata aaataagatt cactgaatat caagaggaat taataagaaa tttgagtgaa
      541 attggaagaa ctgctggaat aaatggttta tatcgagcag attatggaga taaatctcaa
      601 ttttaatgat acttataata ttaaatatgt ctcaaaattt tttgattaga aaaataggta
      661 tattgaatca gtgacataaa agt
//
LOCUS       DANJJH010000259          680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000259 DANJJH010000000
VERSION     DANJJH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            132..344
                     /locus_tag="PWS53_002989"
     CDS             132..344
                     /locus_tag="PWS53_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873133.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>680
                     /locus_tag="PWS53_002990"
     CDS             506..>680
                     /locus_tag="PWS53_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDL0873134.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDS"
BASE COUNT          245 a          102 c          118 g          215 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc
//
LOCUS       DANJJH010000260          669 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000260 DANJJH010000000
VERSION     DANJJH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            245..643
                     /gene="spxA"
                     /locus_tag="PWS53_002991"
     CDS             245..643
                     /gene="spxA"
                     /locus_tag="PWS53_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0873135.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          238 a           98 c          126 g          207 t
ORIGIN      
        1 acgctgtttc atatgtttag aaaatttgaa ttagatttga aaactctaat ttttaaatga
       61 taaaaaaagc gagaatgctg gttttactgt atattaacct tagataactt tcattttttt
      121 aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat
      181 gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg
      241 cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg
      301 gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag
      361 ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac
      421 acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt
      481 attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa
      541 acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg
      601 acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact
      661 tggaaaaga
//
LOCUS       DANJJH010000261          618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000261 DANJJH010000000
VERSION     DANJJH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(47..418)
                     /locus_tag="PWS53_002992"
     CDS             complement(47..418)
                     /locus_tag="PWS53_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL0873136.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT          200 a          116 c           80 g          222 t
ORIGIN      
        1 caggagtact tttttgatat acttttatta atgctgggcg taatggttaa aagatttcca
       61 ctagttcctc tccaccaagc tgagttgaaa aatgcttatc tgtaaacaac atagacaaag
      121 aggaaataaa cgtttccgta tctttctctg tcccaaggat attaattaat tttcctacta
      181 ggtaagtatc attaaactta agatctgcct tcacatcaaa agttttgata aaagctttta
      241 tcatgcgtac aattttgtct ttatcgccat aattgatttc accactcata tcaatatagt
      301 cactaatccc tgaagaaata atggcaactg cttcttcttt cgccttatca gataattcat
      361 ttaatgactg ggattgattg atgacttctt gagttttatc tgttacgtaa tctctcatta
      421 tcctatcggc atactccgca aagtaatctt gaaaaaattc ttctacaggg taaccaactt
      481 cgttttccca accaggagaa atttcattat cagtacttat taaatctatt aaaaaccaac
      541 ccgttctctg agtgttgatt ctataatatt tttaatttcc tatataaatc aaaaatatta
      601 tagaatcaat aagctaat
//
LOCUS       DANJJH010000262          605 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000262 DANJJH010000000
VERSION     DANJJH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>605
                     /locus_tag="PWS53_002993"
     CDS             <1..>605
                     /locus_tag="PWS53_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0873137.1"
                     /translation="SVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFF
                     DKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVI
                     WMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDY
                     YSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTV"
BASE COUNT          187 a           76 c          119 g          223 t
ORIGIN      
        1 ctctgtagct ttgatgcaga cattatattt tgatgataaa tatgttggca gagtagtcgg
       61 cggaacactg tcatttgtgt tgataggttt agtaatttat ttaagaataa tgataaaggg
      121 tgctcatttt tttgataaaa aacagtggag atacgcttta aaaatctctt tgccgctaat
      181 cccacattcc ttagcaaata tagttttagc acaattcgat agaattatga ttaattctta
      241 tttaggtcct gctgcggggg gaatatattc ctacattagt aacataggta ttattctaag
      301 cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg agatgagtaa
      361 aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct ttactttgat
      421 tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc cgaaagacta
      481 ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc agtttttata
      541 tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg cgattggtac
      601 agtag
//
LOCUS       DANJJH010000263          557 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000263 DANJJH010000000
VERSION     DANJJH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..510
                     /locus_tag="PWS53_002994"
     CDS             <1..510
                     /locus_tag="PWS53_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873138.1"
                     /translation="AGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYE
                     LVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMT
                     INSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVK
                     IAESLDDIL"
BASE COUNT          221 a           64 c          105 g          167 t
ORIGIN      
        1 gctgggtcaa gaaccgcatt gcaactttcg gatattttta accctatgat agatatttat
       61 aaagaaaaaa aatatatatt ttttacgtct gtttatgatt ttgagggcgg agaacctgtc
      121 ggagaatatg aattagtatg caaaatagca gacttagtag gaaaagataa tctaattgtt
      181 aagacgcatc ctagagatgt aagaacaatt tataaagata atggatttat cgaggataaa
      241 aattcatcaa taccttggga ggcgattcaa ttgtcaggcg attttaacga taaaatattt
      301 atgacaatta attcaggaag tgttatttca ggtagtacaa tgtctgcaaa acctgtacgc
      361 acgtattata tgtataaatt gtgtaacata gaaggaaatc cgtcttgcaa aaagaatgcg
      421 tatgatattg agcgattgct taagcaaaaa acgatgaaaa acattttaaa gaatgtgaaa
      481 atagcagaaa gcttagatga tatactatga gcaaaactaa atatttaatt aaaaatatgg
      541 gaatattaac aataagt
//
LOCUS       DANJJH010000264          550 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000264 DANJJH010000000
VERSION     DANJJH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>550
                     /locus_tag="PWS53_002995"
     CDS             <1..>550
                     /locus_tag="PWS53_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0873139.1"
                     /translation="YYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQ
                     YHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLS
                     NLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSW
                     NEETSSMTDIQLYDEVMHDNNYS"
BASE COUNT          203 a           65 c           96 g          186 t
ORIGIN      
        1 atattatgat gaaataatcg aagtagctaa aaagaaagat agattacttt atctatcttc
       61 tacatttttg aagagaaaag aaattcaata tattaagtct aaagtgacgc ataataaaaa
      121 aatttcttat caataccatg taggacagta tttacccgac tggcatcctt gggaagatta
      181 tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt agagaaatat tcgctattga
      241 attaccttgg attatagata catttgggaa agttaaatcg tttactgtaa tgacccagaa
      301 attatccaat cttgatattg attattcgga cagtttcact cttattattg agcatgaaac
      361 tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac
      421 tataattggt gaaaagattt taatagaatg ggaaggtaac ccggattctc tattttcatg
      481 gaatgaagaa acttctagta tgacagatat tcaattgtac gatgaggtta tgcatgataa
      541 taattattca
//
LOCUS       DANJJH010000265          526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000265 DANJJH010000000
VERSION     DANJJH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWS53_002996"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="PWS53_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL0873140.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="PWS53_002997"
     CDS             complement(349..>526)
                     /locus_tag="PWS53_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873141.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DANJJH010000266          510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000266 DANJJH010000000
VERSION     DANJJH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>510
                     /locus_tag="PWS53_002998"
     CDS             <1..>510
                     /locus_tag="PWS53_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873142.1"
                     /translation="VNRKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIERE
                     HHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEI
                     LDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQI
                     EGEDKEKRID"
BASE COUNT          209 a           55 c           96 g          150 t
ORIGIN      
        1 gttaatcgaa aaagtacgac taaatttgca aactcattct ggtttttaaa aaatattaat
       61 aaaacagtgg aaaagaatgg agtttataaa ggtgattttc attttcaaaa tgctgagact
      121 attgaacgag aacatcatta tttcgattta ttagtgaata cagtggtaga attagcgaaa
      181 tgggcaaagt caaacgaaac ttatagtcaa gtacttaaaa gaattatgag tcaagaaact
      241 tcatttatct atgagttaag caagtcaaat ttgaaatata cttataatga agatgaactt
      301 gaaattttag ataatccaga aaattcacaa tggattaaag agttgaagag agaagacttt
      361 attgagacac taagaattct aaaatcgtta tttgatttga aattagccaa agctaccatc
      421 atgggaattg aaagagattc atattattgg gaggaaattg gactatatgc tccccagatt
      481 gaaggtgaag ataaagagaa aaggatagac
//
LOCUS       DANJJH010000267          485 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000267 DANJJH010000000
VERSION     DANJJH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(62..259)
                     /locus_tag="PWS53_002999"
     CDS             complement(62..259)
                     /locus_tag="PWS53_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0873143.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DANJJH010000268          484 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000268 DANJJH010000000
VERSION     DANJJH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
BASE COUNT          159 a           96 c           57 g          172 t
ORIGIN      
        1 gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat tacgttatta
       61 aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact tgaagggatt
      121 taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc aaacagtagg
      181 atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt agatgtgccg
      241 ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc aatcgaacga
      301 aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa gacatctcga
      361 ttcatattca agataggaat ctttttatca acatcacttt attcgtattt tcctctacca
      421 taaaagcttc acgctccaca aaattgtttt atacactact attcagccca ttcaacgacc
      481 aatg
//
LOCUS       DANJJH010000269          444 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000269 DANJJH010000000
VERSION     DANJJH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..>444
                     /locus_tag="PWS53_003000"
     CDS             <1..>444
                     /locus_tag="PWS53_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873144.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTD"
BASE COUNT          183 a           65 c          103 g           93 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aaggcgaaaa agtagaagtt
      421 ggaggcacag tagatctaac agat
//
LOCUS       DANJJH010000270          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000270 DANJJH010000000
VERSION     DANJJH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..339)
                     /locus_tag="PWS53_003001"
     CDS             complement(<1..339)
                     /locus_tag="PWS53_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873145.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANJJH010000271          377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000271 DANJJH010000000
VERSION     DANJJH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(<1..132)
                     /locus_tag="PWS53_003002"
     CDS             complement(<1..132)
                     /locus_tag="PWS53_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0873146.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          133 a           69 c           77 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatag
//
LOCUS       DANJJH010000272        19313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.053, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000272 DANJJH010000000
VERSION     DANJJH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     misc_binding    25..241
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            289..2934
                     /locus_tag="PWS53_003003"
     CDS             289..2934
                     /locus_tag="PWS53_003003"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL0873147.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3244..4557
                     /locus_tag="PWS53_003004"
     CDS             3244..4557
                     /locus_tag="PWS53_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL0873148.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4547..5200
                     /locus_tag="PWS53_003005"
     CDS             4547..5200
                     /locus_tag="PWS53_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0873149.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5255..5806
                     /gene="radC"
                     /locus_tag="PWS53_003006"
                     /pseudo
     CDS             5255..5806
                     /gene="radC"
                     /locus_tag="PWS53_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5937..7022
                     /locus_tag="PWS53_003007"
     CDS             5937..7022
                     /locus_tag="PWS53_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0873150.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7019..8911
                     /locus_tag="PWS53_003008"
     CDS             7019..8911
                     /locus_tag="PWS53_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0873151.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8918..9295
                     /locus_tag="PWS53_003009"
     CDS             8918..9295
                     /locus_tag="PWS53_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0873152.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9449..10231
                     /locus_tag="PWS53_003010"
     CDS             9449..10231
                     /locus_tag="PWS53_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HDL0873153.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="PWS53_003011"
     CDS             complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="PWS53_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HDL0873154.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11146..11205)
                     /locus_tag="PWS53_003012"
     CDS             complement(11146..11205)
                     /locus_tag="PWS53_003012"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HDL0873155.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            11597..12259
                     /locus_tag="PWS53_003013"
     CDS             11597..12259
                     /locus_tag="PWS53_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0873156.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            12494..12637
                     /locus_tag="PWS53_003014"
                     /pseudo
     CDS             12494..12637
                     /locus_tag="PWS53_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            12787..12987
                     /locus_tag="PWS53_003015"
     CDS             12787..12987
                     /locus_tag="PWS53_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0873157.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(13241..14920)
                     /locus_tag="PWS53_003016"
     CDS             complement(13241..14920)
                     /locus_tag="PWS53_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0873158.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(14922..15134)
                     /locus_tag="PWS53_003017"
     CDS             complement(14922..15134)
                     /locus_tag="PWS53_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0873159.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            15527..17257
                     /gene="efrA"
                     /locus_tag="PWS53_003018"
     CDS             15527..17257
                     /gene="efrA"
                     /locus_tag="PWS53_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL0873160.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            17254..19014
                     /gene="efrB"
                     /locus_tag="PWS53_003019"
     CDS             17254..19014
                     /gene="efrB"
                     /locus_tag="PWS53_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL0873161.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19109..19306
                     /locus_tag="PWS53_003020"
     CDS             19109..19306
                     /locus_tag="PWS53_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873162.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         6398 a         3086 c         4018 g         5811 t
ORIGIN      
        1 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
       61 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
      121 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
      181 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
      241 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
      301 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
      361 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
      421 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
      481 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
      541 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
      601 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
      661 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
      721 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
      781 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
      841 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
      901 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
      961 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1021 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1081 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     1141 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     1201 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     1261 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     1321 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     1381 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     1441 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     1501 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     1561 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     1621 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     1681 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     1741 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     1801 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     1861 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     1921 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     1981 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2041 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     2101 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     2161 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     2221 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     2281 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     2341 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     2401 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     2461 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     2521 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     2581 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     2641 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     2701 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     2761 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     2821 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     2881 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     2941 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3001 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3061 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     3121 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     3181 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     3241 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     3301 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     3361 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     3421 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     3481 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     3541 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     3601 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     3661 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     3721 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     3781 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     3841 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     3901 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     3961 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4021 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4081 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     4141 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     4201 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     4261 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     4321 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     4381 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     4441 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     4501 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     4561 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     4621 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     4681 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     4741 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     4801 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     4861 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     4921 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     4981 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5041 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     5101 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     5161 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     5221 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     5281 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     5341 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     5401 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     5461 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     5521 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     5581 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     5641 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     5701 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     5761 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     5821 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     5881 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     5941 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6001 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6061 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     6121 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     6181 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     6241 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     6301 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     6361 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     6421 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     6481 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     6541 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     6601 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     6661 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     6721 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     6781 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     6841 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     6901 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     6961 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7021 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7081 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     7141 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     7201 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     7261 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     7321 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     7381 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     7441 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     7501 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     7561 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     7621 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     7681 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     7741 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     7801 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     7861 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     7921 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     7981 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8041 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     8101 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     8161 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     8221 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     8281 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     8341 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     8401 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     8461 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     8521 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     8581 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     8641 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     8701 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     8761 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     8821 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     8881 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     8941 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9001 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9061 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
     9121 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
     9181 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
     9241 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
     9301 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
     9361 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
     9421 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
     9481 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
     9541 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
     9601 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
     9661 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
     9721 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
     9781 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
     9841 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
     9901 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
     9961 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    10021 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    10081 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    10141 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    10201 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    10261 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    10321 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    10381 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    10441 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    10501 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    10561 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    10621 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    10681 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    10741 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    10801 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    10861 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    10921 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    10981 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    11041 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    11101 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    11161 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    11221 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
    11281 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
    11341 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
    11401 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
    11461 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
    11521 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
    11581 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
    11641 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
    11701 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
    11761 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
    11821 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
    11881 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
    11941 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
    12001 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
    12061 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
    12121 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
    12181 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
    12241 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
    12301 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
    12361 tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag taccttccac
    12421 ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg taatttactc
    12481 ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga tacaaaagtg
    12541 tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca aacgatattt
    12601 tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg
    12661 aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa catctgttgc
    12721 acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca cgaggaggat
    12781 attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc
    12841 tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg tgacggattc
    12901 aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga ccgcggccca
    12961 caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat ggtagacatc
    13021 aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga caatttctct
    13081 tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca
    13141 ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct
    13201 cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca
    13261 ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg
    13321 cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc
    13381 ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt
    13441 agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca
    13501 ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga
    13561 cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc
    13621 cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc
    13681 agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga
    13741 attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt
    13801 cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg
    13861 caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag
    13921 gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat
    13981 tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg
    14041 tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt
    14101 tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag
    14161 cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag
    14221 aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat
    14281 gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata
    14341 aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc
    14401 caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt
    14461 tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag
    14521 tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca
    14581 agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag
    14641 gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag
    14701 taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga
    14761 catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga
    14821 tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag
    14881 caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag
    14941 aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca
    15001 atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca
    15061 atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa
    15121 actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga
    15181 tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta
    15241 gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt
    15301 ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac
    15361 agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct
    15421 ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca
    15481 tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg
    15541 gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt
    15601 tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg
    15661 caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat
    15721 cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt
    15781 agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga
    15841 aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt
    15901 gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt
    15961 gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt
    16021 actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca
    16081 aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat
    16141 tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta
    16201 tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt
    16261 cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg
    16321 cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc
    16381 attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg
    16441 tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga
    16501 tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc
    16561 gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt
    16621 tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga
    16681 tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa
    16741 gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc
    16801 agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga
    16861 gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc
    16921 agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat
    16981 catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac
    17041 ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt
    17101 agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga
    17161 agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat
    17221 tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa
    17281 cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca
    17341 gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa
    17401 atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc
    17461 atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg
    17521 atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc
    17581 aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg
    17641 acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat
    17701 gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg
    17761 ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct
    17821 tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa
    17881 gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa
    17941 ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct
    18001 ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt
    18061 ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc
    18121 caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg
    18181 caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa
    18241 gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat
    18301 gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa
    18361 gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat
    18421 ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc
    18481 aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg
    18541 ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat
    18601 gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat
    18661 ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg
    18721 ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca
    18781 agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag
    18841 ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt
    18901 ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa
    18961 ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata
    19021 taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa
    19081 agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga
    19141 agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc
    19201 tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag aagtgtacga
    19261 attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac agg
//
LOCUS       DANJJH010000273        13213 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.080, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000273 DANJJH010000000
VERSION     DANJJH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(204..2162)
                     /locus_tag="PWS53_003021"
     CDS             complement(204..2162)
                     /locus_tag="PWS53_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0873163.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="PWS53_003022"
     CDS             complement(2159..3031)
                     /locus_tag="PWS53_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0873164.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="PWS53_003023"
     CDS             complement(3373..4245)
                     /locus_tag="PWS53_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0873165.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="PWS53_003024"
     CDS             4373..4942
                     /locus_tag="PWS53_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0873166.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="PWS53_003025"
     CDS             5009..5746
                     /locus_tag="PWS53_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0873167.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="PWS53_003026"
     CDS             5977..7245
                     /locus_tag="PWS53_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0873168.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="PWS53_003027"
     CDS             7732..8196
                     /locus_tag="PWS53_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0873169.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="PWS53_003028"
     CDS             8180..8809
                     /locus_tag="PWS53_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0873170.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWS53_003029"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="PWS53_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0873171.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="PWS53_003030"
     CDS             10688..11134
                     /locus_tag="PWS53_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0873172.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="PWS53_003031"
     CDS             complement(11333..11848)
                     /locus_tag="PWS53_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0873173.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="PWS53_003032"
     CDS             11965..13167
                     /locus_tag="PWS53_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0873174.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DANJJH010000274        10489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.094, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000274 DANJJH010000000
VERSION     DANJJH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            192..1088
                     /locus_tag="PWS53_003033"
     CDS             192..1088
                     /locus_tag="PWS53_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0873175.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1238..2179)
                     /locus_tag="PWS53_003034"
     CDS             complement(1238..2179)
                     /locus_tag="PWS53_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0873176.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2305..3426
                     /locus_tag="PWS53_003035"
     CDS             2305..3426
                     /locus_tag="PWS53_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0873177.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3684..5186
                     /gene="eat(A)"
                     /locus_tag="PWS53_003036"
     CDS             3684..5186
                     /gene="eat(A)"
                     /locus_tag="PWS53_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0873178.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5214..5780
                     /locus_tag="PWS53_003037"
     CDS             5214..5780
                     /locus_tag="PWS53_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0873179.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5991..7052
                     /locus_tag="PWS53_003038"
     CDS             5991..7052
                     /locus_tag="PWS53_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0873180.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7055..7951
                     /gene="murQ"
                     /locus_tag="PWS53_003039"
     CDS             7055..7951
                     /gene="murQ"
                     /locus_tag="PWS53_003039"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0873181.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7965..9947
                     /locus_tag="PWS53_003040"
     CDS             7965..9947
                     /locus_tag="PWS53_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0873182.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10012..10458)
                     /gene="nrdI"
                     /locus_tag="PWS53_003041"
     CDS             complement(10012..10458)
                     /gene="nrdI"
                     /locus_tag="PWS53_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0873183.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3433 a         1855 c         2260 g         2941 t
ORIGIN      
        1 taacaaattt tcagacaatt acaactattc agaaaacttt taggcgaatt ttaatagaag
       61 aatgatttat ttcatcatat tttcatacaa atgttaaatt ttcatacaaa tataggaaac
      121 gttttactgg ctttttacta tttaaacagg ctatcatagg aaaaagtgac agagaaagtg
      181 aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc aaaaaataaa tcactttatt
      241 ttttgccttc catccgctct ttagatgttt ggtccggaaa agatttacta cagtcggaat
      301 tcattaaatc agctagacaa cagttggcaa attatcagct gatcgttttt ttagattatg
      361 gatttgatat ggagatggct cgacgcgtgc gcccatttac caatgcaaaa attgtgttat
      421 ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca caagatgaac
      481 cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt gggctgaaac
      541 ataatagttc attttattcg aaacatatga atttttcttc tagcgaacca aagacggatc
      601 tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat aagcaggaat
      661 ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg aatgaacaac
      721 ctggatatct tacttatgaa gagtacctga aaaaaacagg ggaatcgcgc gggattctgg
      781 aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatcc atgtattttc
      841 aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat cgtcctgaaa
      901 atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg catacaccat
      961 ttaagccaat cctatcagag ctattggaat tttatgatgc agaaaattgg gcaaaacgat
     1021 ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc tctgttgaaa
     1081 acaaataaaa aacctacatt ttgaagagat tatgtactga aacaaacaag cacaattctt
     1141 atggcatcat gagagttgtg ttttcttttt gatcttttat tgggcttaaa aaaagagaac
     1201 cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca aggattcctt
     1261 cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata tcatattttt
     1321 gtccttttcg ccaaatggca ccattgatgt agtcgatttc agtcaaacga tggtttttga
     1381 tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca taagtagaag
     1441 caatatgttg gtacacttct tcttgatcta gtatgattcc ttcttttgct gcgatagaag
     1501 caaattcaga tacgattgtt cgaaccatgc tctctgccgc tttttgcgcg cctagttcag
     1561 caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca caagcttttc
     1621 gccaaatgga ataacgaacg ttatggctat agatcggatt taaatttgct tcttgaaaga
     1681 cttctacaac tttttttgta aatgcttcgc cttctggttc caagttctct agttcgattt
     1741 ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc cacatcgtga
     1801 tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca tgacctaatc
     1861 catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc gaacaaaaca
     1921 tatcatctaa ttgattggct tttgttaatg ctacgatcaa gtccacgtgt tcgttgcttt
     1981 cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta ccgttaaagt
     2041 cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga tcgatcaaga
     2101 ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt gctccggccc
     2161 cagctattgc gattttcatg ttttttctct cctttattca atgagttccc ttttagtata
     2221 tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac
     2281 ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc atttaaagga
     2341 agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga tcgtaaagga
     2401 agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc aatctattac
     2461 gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg tcagcgaaaa
     2521 atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga atctgcacaa
     2581 gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact tgcttccagt
     2641 tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca aattattgtc
     2701 actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc cttgggtgct
     2761 tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact tcattttgat
     2821 cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt taccaatcca
     2881 tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg aatagatacg
     2941 acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa agatttctgg
     3001 gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg tggagccctt
     3061 tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat cactggattg
     3121 gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat cgatcagcaa
     3181 acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata tcgaaaacca
     3241 ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca
     3301 attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa
     3361 acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg
     3421 aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga
     3481 gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg
     3541 tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg
     3601 tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt
     3661 ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct
     3721 acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga
     3781 aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa
     3841 acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac
     3901 agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga
     3961 tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag
     4021 aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg
     4081 atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa
     4141 aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc
     4201 ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac
     4261 tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa
     4321 tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa
     4381 aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg
     4441 atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga
     4501 aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat
     4561 taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc
     4621 gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc
     4681 attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt
     4741 catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc
     4801 agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt
     4861 tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa
     4921 agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac
     4981 ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg
     5041 aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg
     5101 tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg
     5161 agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa ggtatggtta
     5221 aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga cacggagaaa
     5281 caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag
     5341 gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga
     5401 aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt gctgatcgtc
     5461 tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg gattatgatg
     5521 gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct gtacgattcc
     5581 caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg aaagaatgca
     5641 tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata agagtgatca
     5701 atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta gataatacag
     5761 aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat ttcaaggcac
     5821 tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa tataaactca
     5881 acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt ttctttagat
     5941 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
     6001 tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
     6061 catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg
     6121 catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga
     6181 tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc
     6241 ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga
     6301 ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag
     6361 atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt
     6421 attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc
     6481 tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc
     6541 cactatatca aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg
     6601 ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag
     6661 aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
     6721 aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg
     6781 atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag
     6841 caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc
     6901 gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg
     6961 tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga
     7021 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     7081 aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc
     7141 cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac
     7201 aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat
     7261 ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc
     7321 gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat
     7381 gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa
     7441 gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     7501 tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg
     7561 caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc
     7621 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa
     7681 tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga
     7741 tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac
     7801 agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct
     7861 ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga
     7921 agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac
     7981 tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag
     8041 aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa
     8101 atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc
     8161 aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag
     8221 gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat
     8281 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     8341 aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga
     8401 ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     8461 tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct
     8521 tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt
     8581 ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga
     8641 atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg
     8701 ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata
     8761 aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg
     8821 gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg
     8881 gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat
     8941 tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa
     9001 tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac
     9061 aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa
     9121 tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag
     9181 taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg
     9241 cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt
     9301 tgatcccatt gatctctgac ggtaaatggt taggctacat ccttggatta ttggcagctt
     9361 atgctggtgg cttcgttgct acattcttct tcggtattcc taaagaccgt ttagaaaaag
     9421 aagaattagc gactgaaaca gccacagtaa atgaagtgcc tgaaaaagta gtacctcaaa
     9481 cttcagctga aagtgtgact atcctctcat cagttgctga cggaacagtt gtttctttag
     9541 ataaagcaag tgatccagtt tttgcacaaa agatgatggg tgaaggctac tttgtagaac
     9601 cagaaaacgg acatatctat tctccagtta ctggggtagt cagctctgtc ttcccaacga
     9661 aacacgctat tgggatcaca acgccaaatg gtttagaaat cttgcttcat atgggaatca
     9721 acacagttga cttaggcggt acgccatttg atgtgaaagt aacagaaggg cagcaagtga
     9781 cacctgatac gcttgtagca gaagctgact tagaagcgat caaagcagca ggcaaagaaa
     9841 catccatgat cgtcttggta accaatatgg atcgtgtaaa aaactttgtc ttggaaaaaa
     9901 caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa agcttaatat gatagaaaca
     9961 atagaaacga aagaagaaac tgaaatctat ttttcagctt cttctttttt attatttatt
    10021 gttttctgct aaaatctgat aaattcgtgt cacatcagat ggtgttcccc gtaattcata
    10081 atctgccaag aaaggaaagc caaatgtttg tgcatattgt ttggctgtta gacaatactg
    10141 attattgaag tttttatttc cgctaccgac tacaccaaga caaagtttat gattgtcttc
    10201 atgttccaga tattcacgca tcgtttcggt caagatttct gtatcaccgt tatctaaacc
    10261 atttccgccg tctaaataag taggaacgaa agtaaaaaat ggttctgttt ctttcgtaaa
    10321 atcactattt tcatggattt cttttaaagt aacttcagga ttgacaggat cttctgcatg
    10381 cattttttca gcatattctg ctagatgttt ggcaaaggca cgcgtatttc ctgaaatcga
    10441 tatatataaa atgttcatgg atcatcctcc aattctttcc ttttagtat
//
LOCUS       DANJJH010000275         8061 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.112, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000275 DANJJH010000000
VERSION     DANJJH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            128..1564
                     /locus_tag="PWS53_003042"
     CDS             128..1564
                     /locus_tag="PWS53_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0873184.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="PWS53_003043"
     CDS             1753..4200
                     /locus_tag="PWS53_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0873185.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="PWS53_003044"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="PWS53_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL0873186.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="PWS53_003045"
     CDS             complement(5690..6793)
                     /locus_tag="PWS53_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0873187.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWS53_003046"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="PWS53_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0873188.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DANJJH010000276         5906 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.127, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000276 DANJJH010000000
VERSION     DANJJH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            212..709
                     /gene="dfrG"
                     /locus_tag="PWS53_003047"
     CDS             212..709
                     /gene="dfrG"
                     /locus_tag="PWS53_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0873189.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(781..2733)
                     /locus_tag="PWS53_003048"
     CDS             complement(781..2733)
                     /locus_tag="PWS53_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0873190.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2740..2976)
                     /locus_tag="PWS53_003049"
     CDS             complement(2740..2976)
                     /locus_tag="PWS53_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0873191.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3211..4014)
                     /locus_tag="PWS53_003050"
     CDS             complement(3211..4014)
                     /locus_tag="PWS53_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0873192.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(4030..4146)
                     /locus_tag="PWS53_003051"
     CDS             complement(4030..4146)
                     /locus_tag="PWS53_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL0873193.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(4391..5320)
                     /locus_tag="PWS53_003052"
     CDS             complement(4391..5320)
                     /locus_tag="PWS53_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0873194.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(5337..>5906)
                     /locus_tag="PWS53_003053"
     CDS             complement(5337..>5906)
                     /locus_tag="PWS53_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0873195.1"
                     /translation="HGKKYTYELAEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQ
                     FYVQLVSQYLTDTSPTEFDDETVQVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWV
                     YDKAGISLPRVAQDQYDATQEISMEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFY
                     HAGDPIGYGDLSSRYWQDHLIGARRVIHN"
BASE COUNT         1771 a         1090 c          966 g         2079 t
ORIGIN      
        1 ttttaactta cataccaaat tacatacaaa atgatttata aaatgcaatt gaaaatgata
       61 attgaatgaa aatttgtgat tttgtggtat tatgataata taacttatag aaaaaattgc
      121 ttattcaact aacgggcagg ataatgagaa aaggtttttt gatagaaata agaaattaga
      181 tggtaaatta gttaaaaagg ctgtgttata aatgaaagtt tctttgattg ctgcgatgga
      241 taagaataga gtgattggca aagagaatga cattccttgg aggattccca aggactggga
      301 atatgttaaa aatactacaa agggacatcc gataatatta ggtaggaaga accttgaatc
      361 aatcggaaga gccttacctg acagaagaaa tattattctg acgagagata aggggtttac
      421 ctttaatggt tgtgaaattg ttcattcaat agaagatgtt tttgagttat gtaaaaacga
      481 agaagaaatt tttattttcg gaggagaaca gatttataat ttgtttttcc cttatgttga
      541 gaaaatgtac atcacaaaaa tacatcatga attcgaagga gatacttttt ttccagaagt
      601 gaattatgag gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc
      661 gtataactac tattttcatg tatatgaaag aaaaaactta ttgagttaac gggtgcgtta
      721 attgaagaag aacagtaaag ataaagcaca agagcttggg attactccaa gctctttttg
      781 tcatttgaaa taaattatat ctttccaatc aaattgttta tctgtaatcc ctaatctttg
      841 agcaggtgtc aaaacctttt tatccgctga tttaaaaggg cgacaaaagt tgtagtaagt
      901 tcttagaatt gtaatcgcaa attgagcata cttcggattg aagttagcgt aaatatagct
      961 cttcttatca ccacgagctg ttgttaatgg tctttcaaga gatgatatat aacgtcttat
     1021 ctgttgaata aagctattgg tcgaatggtc attaacattt aatagcatct ttgcgatttc
     1081 gtttggttcc agagcagata aatccgttct acaatcaact gaataaaatc ctttatcctt
     1141 agtggctaat ggatgtttga tagggttatc ggcatatttg cgatatcttt ctccatcttt
     1201 actaatagcc tcttcgtgga attgatgact tgttagtaat tcagttagat aatgagatgc
     1261 caaagtccgt agaaactttg ttttgaatcc tctgatatct ccccaatcga gcagttctgc
     1321 ttttgcttgt ccgaattctt ttaagcattg ttttctgctt ttcgtcttgt taacttggca
     1381 aataaaatga tgtgcatcgc tcaaccttaa ttctttcgtg aatactctat aaaaggcgtt
     1441 tctaatagag aagtcatcat cactaatcat tcgccattcg tcggcattaa ccatttgttt
     1501 aattaaccaa tagtgagcag ttgtagtata aggagcatca acatttaaac catcgacaaa
     1561 tctactccta ttagtaattt tccctaattc gtgaagataa gcattcctat tttcgctatc
     1621 gttagcggat ggttcttgtg gataataaga ccaatctaac ctatcatttt tacgactaaa
     1681 agtattgaga tggtcttctt tgaattttct tgtatcttca tttaattcat ccattgaaat
     1741 attccaatca taggcaacat cagaacggaa cacatatctt gataagacct cagcagatac
     1801 aactatatac gtttgcatat taaggtcttc aaaccccgta tatttctttg aaccttgtcc
     1861 tttcttacgg acgttattta ggtagtagtg catcttgtcc gtattaagcc acatctcgtt
     1921 aaatttcttt gtttgaaggg gttgtgtctc atatcgttct aaaaactcca aacaacgcct
     1981 atacagccac tccagcttat gatagtaggt tccaactcca attcctaaaa tatcacaagt
     2041 acgactgact gaaacccttc caacaaccat cttagcgaac attggaagaa tggtgttctt
     2101 ttgttggtga taagtagtgg tttcttctct ttttggtaga acattagtga acttcttaca
     2161 cgccttacat tgatagcgtt gagatttgcc cctacttttc ccccttttgt aaaattgctt
     2221 cggctcattg aatggggttg attcttcttc cgagcagcct tctttatgaa attgataatc
     2281 tggctcaaca tcttggatgc tattgatttc tataaggcgt ttaatttctt caacaacaga
     2341 ccaattagat aaaggggtta ctgtacagtt ttgcgataca cctctgccaa taggattgtc
     2401 attgcagtac atacctttat gacctttatc tttggaacta ccagtcattt tatatcgact
     2461 tggtttccct tttacatctt tgaatttgtg ctgttctttc ccaaaattac aacaaaacgg
     2521 attgatgcag taattatatt gaatgggata ttcagaaccg ttccatttaa aagacgttgg
     2581 caagaacaac attttgttat accttgttgc aaatcgtttt ggagttaata aatcatattg
     2641 tttagcccta tcttttaatt cttcgtttga aatcggaatt ttaacatcta caatttcatc
     2701 ttccttcgtg gctaatcttt taagcttggt cattgtctat caacccactc tatctttgat
     2761 acccattttt tcaacaatcc gcttattact tcgcttgttg gcaatccatt caataaactt
     2821 cttatcgtct aaaatgttca ataagaattc atccactgct tcgctttcag tacgctctgc
     2881 atattcagcg tattgcttaa ctatcaccct tacttgctct gatatttccc aatccacaga
     2941 cttagcgttc ttattttttg gctcaagaaa ttgcatagga aaatgcaccc ctttttgctt
     3001 agtaaattgt aaccatttta acacaccaat ttgcattcct acaataatta tttcacgttc
     3061 gcatttttat atacatttta acttacattt tgtggtgtaa attaacaaca aacgttgatt
     3121 taacaataaa aaacgagtat caaattcagt tgcaatttga tactcattta ggtttacatt
     3181 ttcacttttt gtactccaaa actgaactac ttaatatgca agacgttgtc ttttttttgt
     3241 atattcaatt ttgaaaacat ttccgcaaag ttcagacgga cctcgatgtg ttgatgaata
     3301 aaatttatta gtaataactt ctataaggtt atcaatccct atattacttt ttgcacttcc
     3361 atgataaaga gggaacagag aacaattctg aaatcttatg ctttcctctt gttcgagttc
     3421 caatgcttct aatgatttac cggacatata tttctctaaa aggtcatcgt ttccctctat
     3481 taccgtatcc cattgttcag attcggtaaa gttcgtcaca cacatattag gatacagttc
     3541 taccttctgt ttgattacaa tttcggcaga aagtttctct ttaatatcct gataaaccgt
     3601 tgataaatca attccatttt ggtcaatctt attgataaaa aagattgtgg gaatccccat
     3661 tttcctaagt gcatgaaata atatacgagt ttgtgcttgt acgccatctt ttgcagaaat
     3721 cagtagaatt gccccatcta aaactgataa tgaacgatat acttctgcta agaaatccat
     3781 atgtcctggc gtgtctatga tgttcacctt cgtattttcc cactgaaaag aggttattcc
     3841 tgtctgaatt gtaattcctc tctgacgttc taaaagcgta ttatccgtcc tcgttgtacc
     3901 tttgtccacg cttcctaatt ctgtaatcgc tccactgtta tataataagc tttctgttaa
     3961 ggtagttttt cctgcatcaa catgagctaa aactccaata ttaataattt tcatgtgatt
     4021 ttcctccatt caaaaaccca aaagggcata aaaatcccag tgataaatac ttttatcact
     4081 gggattttta tgcataacca taggtataca aagcatacag atattctctg gatactttag
     4141 aatcacatga taaaggtatt cttaaactgg gtacaaaaaa ctaagccctc ctaaaaaagg
     4201 acatctaatt atttgttccc gctatcaaat tgacagttta tttaagaata ccttgccgca
     4261 tatttattaa ctccttttaa atagtcactt aaataatagc acgtaagagc atatttgtaa
     4321 aggaatctcc aattttttat caaaaagagt acatgattac aaagtatctg taatcatgta
     4381 ccaatatttg ttattttaca atcttccaat tatcttgttt ttctaatatc aactcaaatt
     4441 gcgagatttg ggttgccttt gtttcttgat ctaaatattt cacagcgaca ttcacgatta
     4501 cttggttatc ttttctggta aagataggat tcaccaattc ctcgaaaaca tattccttgt
     4561 taatcatagg taacacatga ttacttacat agtaagtcaa ttctttttct gtggcggtag
     4621 gatagagctg gaagaacgtt tctaaaaagc tagtgatttc gtccatcgtt tctgtatcta
     4681 ctgtatggtc actttctagc tgtttgggtt ggtagtccga tttttgaggt ttcttactca
     4741 ttgtcggatt tttgataata accatattat ctgattcatc tatatggaca accacatgaa
     4801 agctagacga tatagtttcc ttgtctttgt cttcggtaat aacttgttca acagaaaaca
     4861 gtacctcaaa ggtgttctca ttcacttgag aaacctgcca cacctgtata tcgttcacac
     4921 tagaactagt aggaatatcc ttacgaatca tttcttcatt taatacctgc aattcttccg
     4981 tcaaataatg ggtcaatttt tcatttcgtt tatcaatggc ttcttgggat tgttgccacg
     5041 aaaagtagtc tttcgcaaag gatttcacaa aactttctac ttggttggta tcgacaatct
     5101 gctgtttaat gatttctgtt tcacggacag tatgagtgtc aatggcagta aaatttttat
     5161 agattccaaa tccgacacta ccaatcaata aaacccataa aacaaaggtt aatttcttat
     5221 gcgtgccaac tttaatcatc ggaacttttc tttgcttgct cggtttttta gatttctttg
     5281 gtttctcctt cttttctttt cgctcgattt ttataatcat ttgattcatt cctttcttag
     5341 ttatgaataa cacgtctagc cccaatcaga tggtcttgcc aataacgact gcttaagtcg
     5401 ccataaccga ttgggtcgcc tgcatgataa aagcgatttc cctctaaata tatcgccaca
     5461 tgagtcacat atgtgcctgc attataggtt gaatgaaaga agataaggtc gcctgcttgg
     5521 gcttcttcca tagagatttc ttgcgtagcg tcatactgat cttgggcgac acgagggaga
     5581 gaaatgccag ctttgtcata cacccattgt attaagccac tacaatcaaa agaggtggta
     5641 ggcgacgccc ctccaaagac atagggaaac ccttcgtatt tcaaggcttc gtccataatg
     5701 acttgcacgg tttcatcatc aaattctgtt ggggaagtgt cggttaggta ttgtgaaacc
     5761 aactgaacat aaaactgatt tccatagtta tatcgccaac caccattcac aggtatggct
     5821 ataggattgg gatagtctgc tttttgacca cccgacttct ctttagaaaa ctgttcggct
     5881 aattcatagg tatatttttt cccatg
//
LOCUS       DANJJH010000277         5613 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.130, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000277 DANJJH010000000
VERSION     DANJJH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            complement(40..1026)
                     /locus_tag="PWS53_003054"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="PWS53_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="PWS53_003055"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="PWS53_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0873196.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="PWS53_003056"
     CDS             1851..3005
                     /locus_tag="PWS53_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0873197.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="PWS53_003057"
     CDS             3220..4188
                     /locus_tag="PWS53_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0873198.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="PWS53_003058"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="PWS53_003058"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0873199.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="PWS53_003059"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="PWS53_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DANJJH010000278         2014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.195, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000278 DANJJH010000000
VERSION     DANJJH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            186..1226
                     /locus_tag="PWS53_003060"
     CDS             186..1226
                     /locus_tag="PWS53_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0873200.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1324..1512
                     /locus_tag="PWS53_003061"
     CDS             1324..1512
                     /locus_tag="PWS53_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL0873201.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1570..1923)
                     /locus_tag="PWS53_003062"
     CDS             complement(1570..1923)
                     /locus_tag="PWS53_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0873202.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          663 a          320 c          362 g          669 t
ORIGIN      
        1 atcattttgt atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt
       61 acattttgtc attttgtact ccaaaactga actacttact tgcatatata cgcctttata
      121 gtggagtact acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta
      181 cagaaatgta tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag
      241 aaattgttat tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac
      301 tattgccaca gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac
      361 cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg
      421 atccgcttct acgatattac gtggattcta cgacacatga aattatactt tctttcttag
      481 ggaaagtaca aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag
      541 aactaaaaga gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca
      601 ttcacatcga agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc
      661 ttccgttggg aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat
      721 catttcaaaa tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt
      781 ggaatgtgac ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta
      841 ctccagcaga ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg
      901 gaacagaatt gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt
      961 cacgggcata taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa
     1021 ataatgaggt cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg
     1081 atttaacttt ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg
     1141 ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa
     1201 gatatatgtt caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt
     1261 attaaataag atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt
     1321 gttattataa actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga
     1381 atacgagtgg atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa
     1441 actttaatta atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg
     1501 cagagccgat aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa
     1561 ttttactgac tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat
     1621 taaggatagt tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt
     1681 gcttttaatc aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga
     1741 tacaagatca tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa
     1801 ataacgtggg tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat
     1861 tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg
     1921 catactaaat cacctctata cattttactg ttcctatata accataaaca ggtatagaaa
     1981 aacatgttat atagtttatg agttcctata gaac
//
LOCUS       DANJJH010000279         1504 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.214, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000279 DANJJH010000000
VERSION     DANJJH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            262..345
                     /locus_tag="PWS53_003063"
                     /pseudo
     CDS             262..345
                     /locus_tag="PWS53_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="PWS53_003064"
     CDS             394..477
                     /locus_tag="PWS53_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDL0873203.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="PWS53_003065"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="PWS53_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0873204.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DANJJH010000280         1395 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.224, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000280 DANJJH010000000
VERSION     DANJJH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            31..>1395
                     /gene="aph(2'')-I"
                     /locus_tag="PWS53_003066"
     CDS             31..>1395
                     /gene="aph(2'')-I"
                     /locus_tag="PWS53_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDL0873205.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          585 a          103 c          241 g          466 t
ORIGIN      
        1 gtatatgatt atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata
       61 agaactttaa tagatgatga ttttcctttg atgttaaaat ggttaactga tgaaagagta
      121 ttagaatttt atgatggtag agataaaaaa tatacattag aatcattaaa aaaacattat
      181 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      241 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      301 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      361 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      421 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      481 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      541 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      601 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      661 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      721 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      781 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      841 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      901 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      961 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
     1021 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
     1081 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1141 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1201 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1261 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1321 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1381 gaaactattg tttat
//
LOCUS       DANJJH010000281         1382 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.229, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000281 DANJJH010000000
VERSION     DANJJH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            <1..1311
                     /locus_tag="PWS53_003067"
     CDS             <1..1311
                     /locus_tag="PWS53_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL0873206.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DANJJH010000282         1350 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.231, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000282 DANJJH010000000
VERSION     DANJJH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            47..298
                     /locus_tag="PWS53_003068"
                     /pseudo
     CDS             47..298
                     /locus_tag="PWS53_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="PWS53_003069"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="PWS53_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0873207.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DANJJH010000283         1194 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538675-rid17232833.guided.239, whole genome shotgun
            sequence.
ACCESSION   DANJJH010000283 DANJJH010000000
VERSION     DANJJH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538675
            Sequence Read Archive: ERR10688238
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637974.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637974.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:35:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,927
            CDSs (with protein)               :: 2,927
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 25 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538675-rid17232833.guided.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Blood, central line"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2018-11-20"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="PWS53_003070"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="PWS53_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HDL0873208.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
